• fraction
  • Two different types of low-density detergent-insoluble glycosphingolipid-enriched membrane domain (DIG) fractions were isolated from myelin by extraction with Triton X-100 (TX-100) in 50 mM sodium phosphate buffer at room temperature (20°C) (procedure 1), in contrast to a single low-density fraction obtained by extraction with TX-100 in Tris buffer containing 150 mM NaCl and 5 mM EDTA at 4°C (procedure 2). (wiley.com)
  • The single DIG fraction obtained by procedure 2 gave a single opaque band at a similar sucrose density to B1. (wiley.com)
  • Both B1 and B2 had characteristics of lipid rafts, i.e. high galactosylceramide and cholesterol content and enrichment in GPI-linked 120-kDa neural cell adhesion molecule (NCAM)120, as found by others for the single low-density DIG fraction obtained by procedure 2. (wiley.com)
  • The single low-density DIG fraction obtained by procedure 2 contained only low amounts of actin and tubulin. (wiley.com)
  • isolation
  • Additionally, exosomes have been shown to be part of intercellular communication functions, with implications toward both anti-tumor and pro-tumor activity.6 Previous work has provided insight to the isolation of exosomes using density gradient ultracentrifugation,2,7 although there is an effort to gain more concrete confirmation of the size and concentration after purification. (pharmpro.com)
  • gravity
  • There is a correlation between the size and density of a particle and the rate that the particle separates from a heterogeneous mixture, when the only force applied is that of gravity. (wikipedia.org)
  • shown
  • The amino acid sequence NPXY was shown to be an internalization signal for the low-density lipoprotein receptor ( 5 ), and related tyrosine-containing internalization signals are present in the intracellular domain of various other receptors that are internalized by a pathway involving clathrin-coated pits and vesicles ( 6 , 7 ). (aspetjournals.org)
  • The gradient was an overlaid gradient, as shown in Table 1. (pharmpro.com)
  • lower
  • The two DIG fractions obtained by procedure 1 gave opaque bands, B1 and B2, at somewhat lower and higher sucrose density respectively than myelin itself. (wiley.com)