Cellular mammalian hela cells. Medical search. Frequent questions

Western blot analysis of HeLa cell lysate using Proteasome activator 11S gamma subunit, pAb (Prod. (enzolifesciences.com)
Complementary technologies include cell lysate microarrays in which the protein repertoire of a number of cell populations are spotted in parallel on a slide and assayed for reporters of cellular state [18] [19] although all data are population averages rather than per-cell readouts. (antiviralbiologic.com)

The serine/threonine kinase protein kinase D1 (PKD1) is a stress-responsive kinase and sensor for reactive oxygen species, which can initiate cell survival through NF-κB signaling. (biologists.com)
The reactive oxygen species (ROS), glutathione (GSH), carbonyl content in proteins (CCP), DNA-protein crosslinks (DPC) and cell viability were measured. (scirp.org)

Autophagy is an intracellular catabolic pathway in which cellular constituents are engulfed by autophagosomes and degraded upon autophagosome fusion with lysosomes. (nature.com)
Autophagy serves as a major cytoprotective process by maintaining cellular homeostasis and recycling cytoplasmic contents. (nature.com)
However, emerging evidence suggests that autophagy is a primary mechanism of cell death (autophagic cell death, ACD) and implicates ACD in several aspects of mammalian physiology, including tumor suppression and psychological disorders. (nature.com)
Autophagy is generally considered as a cell survival/protection mechanism because it removes toxic or obsolete proteins and organelles and recycles the degradation products for use as sources for energy and metabolites in anabolic pathways 3 . (nature.com)
Therefore, the relationship between autophagy and cell death remains unclear and warrants further study to harness autophagy for the treatment of various human diseases. (nature.com)
Therefore, in this review, we avoid a lengthy repetition of the description of autophagy and cell death processes and focus on the death-promoting roles of autophagy and the intertwined connection between autophagy and apoptosis. (nature.com)
Proven by the use of apoptosis-resistant cellular models and autophagic assays, such isoquinoline alkaloid-induced cytotoxic effect involves energy- and autophagy-related gene 7 (Atg7)-dependent autophagy that resulted from direct activation of AMP activated protein kinase (AMPK). (oncotarget.com)
Autophagy is a highly coordinated process responsible for maintaining normal cellular homeostasis under nutrient deprivation conditions. (oncotarget.com)
AMP activated protein kinase (AMPK), maintains normal energy balance by regulating cellular metabolisms in an AMP/ADP ratio-dependent manner, is responsible for the proper mechanistic modulation of autophagy [ 2 ]. (oncotarget.com)
During cellular starvation, AMPK induces autophagy by phosphorylating Ulk1, the mammalian counterpart of ATG1, at Ser 317 and 777 [ 3 , 4 ]. (oncotarget.com)

Molecular studies demonstrated that Ulk1 together with another mammalian ATG1 homolog, Ulk2, form complex with mATG13 and FIP200 (mammalian homologues of ATG13 and ATG17) and regulate the autophagic machinery [ 5 , 6 ]. (oncotarget.com)
Both Atg37 and Atg36 have already been reported to become essential regulators of pexophagy in candida, and ACBD5 (acyl-CoA binding site containing 5) continues to be suggested like a mammalian homolog for Atg37 [19,20]. (biotech2012.org)

The fate of the UCNPs within the cells was studied by examining their upconversion emission spectra with confocal microscopy and confirming these observations with transmission electron microscopy. (edu.au)
HeLa cells transiently expressing GlcNAcT-1-HA-KDEL were fixed, immunolabeled for GlcNAcT-1-HA-KDEL (red) and endogenous alpha-mannosidase II (ManII, green), and examined by fluorescence microscopy. (cellimagelibrary.org)
Live-cell fluorescence microscopy (LCFM) is a powerful tool used to investigate cellular dynamics in real time. (biomedcentral.com)
Here we describe a widely applicable nontoxic approach for measuring DNA content in live cells by fluorescence microscopy. (biomedcentral.com)
To overcome this limitation, approaches incorporating laser scanning cytometry and fluorescence microscopy have been successful in quantifying DNA content in single cells [ 2 , 3 ], providing the capacity to combine this information with additional cellular parameters that can be elucidated by imaging. (biomedcentral.com)
Therefore, traditional methods for examining DNA content by microscopy have relied on cellular fixation, which is incompatible with tracking cells over time. (biomedcentral.com)
Cellular organelles had been imaged by confocal microscopy. (biotech2012.org)

Mitochondria and the complex endomembrane system are hallmarks of eukaryotic cells. (biomed.news)
Upon cell-to-cell transplantation, the transferred mitochondria fuse to the host cells mitochondrial network. (biomed.news)
After transfer of mitochondria and cell propagation over generations, donor mitochondrial DNA (mtDNA) was replicated in recipient cells without the need for selection pressure. (biomed.news)
On the other hand, in mammalian mitochondria, the initial step of Fe-S cluster assembly process is assisted by NFS1-ISD11 complex, which delivers sulfur to the scaffold protein ISCU during Fe-S cluster synthesis. (iisc.ac.in)
D) HeLa cells stably expressing turquoise2-Peroxi, mitochondria-YFP, turquoise2-ER, or turquoise2-Golgi had been transfected with Sc or sifor 5 d, stained with DRAQ5, and set. (biotech2012.org)

By culturing the cells on top of NW arrays, the maximum traction forces of two different tumor cells (HeLa, L929) have been measured by quantitatively analyzing the bending of the nanowires. (edu.sa)
A cell line derived from cultured tumor cells. (lookformedical.com)

It also reduces the proliferation of pulmonary artery smooth muscle cells. (biomed.news)
This issue is of particular relevance since changes in NO release could play an important role in endothelial function maintenance, in addition to regulating proliferation of smooth muscle cells, leukocyte adhesion, platelet aggregation, angiogenesis, thrombosis, vascular tone, and hemodynamics. (hindawi.com)
Deletions of multiple GID subunits compromise cell proliferation, and this defect is accompanied by deregulation of critical cell cycle markers such as the retinoblastoma (Rb) tumor suppressor, phospho-Histone H3 and Cyclin A. We identify the negative regulator of pro-proliferative genes Hbp1 as a bonafide GID/CTLH proteolytic substrate. (elifesciences.org)
During these processes, the cancer cells acquire multiple allelic mutations in genes such as proto-oncogenes, tumour suppressor (TS) genes and other genes that control cell proliferation ( Hahn and Weinberg, 2002 ). (kdwis.com)
GSK3 phosphorylates Tauroursodeoxycholate -catenin, that leads to its devastation, hence suppressing alerts that promote cell proliferation in any other case. (globaltechbiz.com)
Subsequently, GSK3 promotes oncogene-induced change and proliferation in leukemia cell lines. (globaltechbiz.com)
GSK3 inhibitors decrease the proliferation of Kinase Assay MCF7 cells had been lysed with Cell Lysis Buffer (Cell Signaling Technology, 9803). (globaltechbiz.com)
Signal transduction plays an important role in activating cellular functions, cell differentiation , and cell proliferation . (lookformedical.com)

In an effort to further understanding of the effects of radiofrequency (RF) radiation on somatic cell mitotic activity and germ cell function, cell suspensions were exposed to 27 megahertz (MHz) or 2,450MHz radiation under isothermal conditions in-vitro. (cdc.gov)
2005 Science Daily obituary November 11, 2005 Rocky Mountain News, obituary November 9, 2005[permanent dead link] Tribute by Dr. Gordon Sato & colleagues, In Vitro Cell. (wikipedia.org)
Because NS is often observed during the mammalian CA test (in vitro), we examined the relationship between NS and CAs. (biomedcentral.com)
We have determined that the ability of C. jejuni to bind to epithelial cell lines in vitro is significantly affected by the growth temperature and growth stage of the bacteria, but not by growth-medium composition. (microbiologyresearch.org)
Indeed, Hbp1 accumulates in cells lacking GID/CTLH activity, and Hbp1 physically interacts and is ubiquitinated in vitro by reconstituted GID/CTLH complexes. (elifesciences.org)
The role of ZIP4 in HCC was investigated by overexpressing ZIP4 in BEL7402 and HepG2 cells and inhibiting ZIP4 in HuH-7 and HepG2 cells, using overexpression and shRNA plasmids in vitro studies. (ijbs.com)
BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C and CDC25B in an in vitro assay. (signalchem.com)
Cells propagated in vitro in special media conducive to their growth. (lookformedical.com)
In vitro, most HRVs and HEVs differ by genotype associated with respiratory symptoms and acute their optimal growth temperature, acid tolerance, receptor otitis media, confi rming the close relationship between rhi- usage, and cell tropism. (cdc.gov)

2017). My intention is to apply this approach now for a quantitative analysis of mitotic spindle structure in mammalian cells. (tu-dresden.de)

Puck's key work ultimately made modern genetics, such as the human genome and other mammalian genome projects, possible. (wikipedia.org)
High-throughput stream cytometry probing immunolabeled phosphoproteins [6] [7] enables multiparameter sampling of proteins activation state across a large cell population but requires serial analysis of samples hence performing sequential assays of every experimental condition or timepoint - a key limitation when performing genome-scale screens. (antiviralbiologic.com)

Nevertheless, the definition of autophagic cell death (ACD) has been neither universally understood nor unanimously accepted in the field 4 . (nature.com)
In conclusion, isoquinoline alkaloid is a new class of compound that induce autophagic cell death in drug-resistant fibroblasts or cancers by exhibiting its direct activation on AMPK. (oncotarget.com)

Mcsweegan E. , Walker R. I. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. (microbiologyresearch.org)
TMDs are embedded in the lipid bilayer with NBDs located in the cytosol, thus having access to cellular stores of ATP that can fuel the export of substrates. (unscburma.org)
10-13 For example, one study found cells expressing mouse P-gp were approximately three- and 22-fold more resistant to actinomycin D and colchicine than cells expressing comparable amounts of individual P-gp.12 These findings indicate which the profile of substrates effluxed by mouse and individual P-gp are overlapping however, not identical. (unscburma.org)

The internalization and cytotoxicity of the thus obtained UCNPs were evaluated in three cell lines (HeLa, HEK293 and astrocytes). (edu.au)

Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)

Here we report the differential localisation of PARP-1 N-terminal fragment encompassing NLS in PARP-1 +/+ and PARP-1 −/− mouse embryo fibroblasts by live imaging of cells transiently expressing EGFP tagged fragment. (molcells.org)

Intro Despite enormous progress in the postgenomic era large-scale characterization of mammalian genes continues to be a daunting problem. (antiviralbiologic.com)

For successful transgene expression, viruses administered into muscle must undergo a series of processes, including host cell interaction and internalization, intracellular sorting, long-range retrograde axonal transport, endosomal liberation, and nuclear import. (frontiersin.org)
Fluorescence imaging is an indispensable method for analysis of diverse cellular and molecular processes, enabling, for example, detection of ions, second messengers, or metabolites. (jneurosci.org)
SIGNIFICANCE STATEMENT FLIM is an indispensable method for analysis of cellular processes. (jneurosci.org)
In addition, cell death subroutines have been recently classified on the basis of mechanical and molecular aspects of cell death processes 5 . (nature.com)
Collectively, we anticipate that this method will allow high-resolution microscopic analysis of cellular processes involving cell cycle progression, such as checkpoint activation, DNA replication, and cellular division. (biomedcentral.com)
This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. (lookformedical.com)
All of the processes involved in increasing CELL NUMBER including CELL DIVISION . (lookformedical.com)

Subsequently, gefitinib, sorafenib, neratinib, tamoxifen citrate, and cyclosporine A enhanced the expression of FN3K in BT-474 cell lines with simultaneous alteration in Nrf2-driven antioxidant signalling. (bvsalud.org)
Then, a survey is given about ATP1A2 mutations implicated in migraine cases as documented in the literature with focus on mutations that were described to completely destroy enzyme function, or lead to misfolded or mistargeted protein in particular model cell lines. (frontiersin.org)
Cell death induced by blasticidin occurs rapidly, allowing for selection of transfected cell lines carrying a blasticidin resistance gene within one week. (invivogen.com)
The working concentration of blasticidin for mammalian cell lines varies from 1 to 10 μg/ml, in a few cases up to 30 μg/ml. (invivogen.com)
Our biochemical results using yeast model system and HeLa cells lines suggests that ISCU Myopathy results in defective Fe-S cluster biogenesis in mitochondrial compartment. (iisc.ac.in)
Coupling this technology to high-throughput methods for culturing and treating cell lines could enable experts to examine the effect of exogenous effectors on the same human population of experimentally treated cells across multiple reporter focuses on potentially representing a variety of molecular systems therefore producing a highly multiplexed dataset with minimized experimental variance and at reduced reagent cost compared to alternate techniques. (antiviralbiologic.com)
For transfection-based screens however the technique is limited to cell lines compatible with the transfection technique used although Sabatini and colleagues Artemisinin Spp1 created arrays based on lentiviral infection constructs to circumvent this problem [16]. (antiviralbiologic.com)
Here we describe the application of spotted cell microarrays to the study of human cell lines. (antiviralbiologic.com)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
Transduced cells and the drug-selected KB-8-5-11 and C3M cell lines were incubated with 1.5 M Rh123 either alone (gray bars) or in combination with the P-gp modulators. (unscburma.org)
Discussion In the current study, we determined the methylation status of the SPARC gene promoter in pancreatic cancer cell lines, pancreatic cancer and corresponding adjacent normal pancreatic tissues, chronic pancreatitis tissues, and real normal pancreatic tissues. (hdacassay.com)

NS measurements were carried out using compounds known to induce structural CAs in the mouse lymphoma L5178Y cell line. (biomedcentral.com)
The results indicated that porous spherical CaCO 3 microparticles may induce oxidative damage to Hela cells. (scirp.org)

WB: Romas and HeLa cell lysates. (abcam.com)
Cell lysates were fractionated and probed for RIPK3 or RAGE. (bioz.com)

1994. Blasticidin S deaminase gene from Aspergillus terreus (BSD): a new drug resistance gene for transfection of mammalian cells. (invivogen.com)
After treatment, cell death occurs rapidly, allowing the selection of transfected cells with plasmids carrying the bsr or BSD genes in as little as 7 days post-transfection. (invivogen.com)

After EGF stimulation for 4 h, Helix Pomatia Lectin (HPL) (green) in HeLa cells expressing constitutively active mutant Arf1-GFP (Arf1(Q71L)-GFP) (gray) resists redistribution from Golgi (Giantin) (re. (cellimagelibrary.org)
GSK3 is certainly a constitutively energetic enzyme in regular cells and goes through fast inhibition by stimuli [2], [4]. (globaltechbiz.com)

In PARP-1 +/+ cells the fragment localises to the nuclei presenting a granular pattern. (molcells.org)

Presented in conjunction with cells expressing a histone 2B-GFP fusion protein (H2B-GFP), we demonstrated how this method enabled chromosomal segregation errors to be tracked in cells as they progressed through cellular division that were later identified as either diploid or polyploid. (biomedcentral.com)

Overexpression of BRSK2 induces G2/M arrest in HeLa cells while small interfering RNA against BRSK2 partly abrogated UV-induced G2/M arrest. (signalchem.com)

RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
After cell lysis, polyadenylated RNAs were captured on oligo d(T) beads followed by stringent washes to remove non-crosslinked proteins. (nature.com)
Cellular proteostasis involves the coordinated and compensatory action of pathways that control biogenesis, folding, trafficking and breakdown of proteins allowing the cell to adapt to physiological or pathological environmental changes. (elifesciences.org)
This HeLa cell is expressing two fluorescent proteins, including EGFP-CENP-B, a fluorescent protein that marks kinetochores (seen as paired dots oriented parallel to the x-axis and attached to opposit. (cellimagelibrary.org)
Cancer cells are known to proliferate uncontrollably and therefore, have greater demand for energy and a ready supply of the building blocks necessary for the biosynthesis of macromolecules such as nucleotides, proteins and lipids). (kdwis.com)
This process involves the lysosomal degradation of cellular components such as misfolded proteins or damaged organelles. (oncotarget.com)

Methods pertinent to homology modeling, virtual screening, molecular docking, molecular dynamics simulations, assessment of ADME properties, cytotoxicity assays for anticancer molecules of natural/synthetic origin in breast cancer cells (BT-474, T-47D), and Western blotting were used in this study. (bvsalud.org)
Metaphase Kinetochore Dynamics: In this HeLa cell, which is expressing both a kinetochore marker (EGFP-CENP-B) and a centrosome marker (Venus-centrin), one can visualize the dynamic nature of kinetoch. (cellimagelibrary.org)
FE65 is functionally linked to cellular motility and morphology and actin dynamics by way of binding of its WW domain to the actin binding protein Mena. (dehydrogenase-signal.com)
This method allows the examination of single-cell dynamics to be correlated with cellular stage and ploidy in a high-throughput fashion. (biomedcentral.com)

promoting abnormal regulation of signalling pathways involved in cell cycle regulation, genetic stability, apoptosis and cell differentiation. (kdwis.com)
Resistance of cancer cells towards apoptosis is the major cause of these symptomatic behaviours. (oncotarget.com)
Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. (lookformedical.com)

the chorion gene loci in follicle cells (21 22 In pre-RC development the effective launching of multiple MCM complexes is necessary for the toleration of replication strains and activation of checkpoint pathways (23-25). (exposed-skin-care.net)
Virus-mediated gene therapy has the potential to deliver exogenous genetic material into specific cell types to promote survival and counteract disease. (frontiersin.org)
Administration of gene therapy viruses into skeletal muscle, where distal terminals of motor and sensory neurons reside, has been shown to result in extensive transduction of cells within the spinal cord, brainstem, and sensory ganglia. (frontiersin.org)
Gene therapy viruses are non-replicating, but still hijack host cell machinery to express transgenes of interest in the nucleus. (frontiersin.org)
We captured early stage interactions of invasive-bacteria and HeLa cells during the process of bacteria-mediated gene delivery and illustrate sufficient clarity can be obtained using this procedure to preserve and clearly visualize relevant cellular structures. (um.edu.my)
L5178Y is often used in mouse lymphoma L5178Y cell Tk (thymidine kinase) gene mutation assays. (biomedcentral.com)
1991. Blasticidin S-resistance gene (bsr): A novel selectable marker for mammalian cells. (invivogen.com)
NF-kB DNA binding activity is certainly reduced and its own focus on gene items, including MMP-9, survivin, IAP-1, BCL-xL, FLIP Tauroursodeoxycholate and TRAF1, are abrogated in GKS3-null cells [16]. (globaltechbiz.com)

After 5 d, the cells had been analyzed and harvested by western blotting using the indicated antibodies. (biotech2012.org)

Some of the impairments in glycolytic pathway, Krebs cycle and oxidative phosphorylation that contribute to cancer cell growth, development, progression, survival and malignancy would be critically discussed. (kdwis.com)
by CG0009 resulted in slight upregulation from the -catenin focus on genes, c-Myc and c-Jun, however, not cyclin D1, indicating that CG0009-mediated cyclin D1 depletion overwhelms the pro-survival sign of -catenin, leading to cell loss of life. (globaltechbiz.com)
SIRT6 Antibody: The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence and lifespan extension. (prosci-inc.com)
Hence for cell survival, maintenance of genomic integrity and longevity presence of intact PARP-1 in the nucleus is paramount. (molcells.org)
Cell survival. (lookformedical.com)
It plays a major role in cell metabolism, growth, and survival as a core component of SIGNAL TRANSDUCTION . (lookformedical.com)

In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)

Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
Cancer Cell Metabolism: Unique Features Inform New Therapeutic Opportunities. (nyas.org)
2014. Multiple myeloma-associatedhDIS3 mutations cause perturbations in cellular RNA metabolism and suggest hDIS3 PIN domain as a potential drug target. (invivogen.com)
In the paper, the relationship between cancer and cellular energy metabolism will be examined. (kdwis.com)
It will elucidate on the additional hallmarks of cancer mentioned above, i.e. reprogramming energy metabolism in cancer cell by pointing out to key experiments pertaining, and that serve as proof, to this phenomenon. (kdwis.com)
Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g. energy metabolism, and thereby are associated with aging and several age-related diseases. (prosci-inc.com)
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. (lookformedical.com)

Molecules and Cells, 46 (1). (cshl.edu)
The finding that PA28 modulates the proteasome-catalysed production of antigenic peptides presented to the immune system on MHC class I molecules indicates a cellular function of this activator in antigen processing. (enzolifesciences.com)
PARP-1 (EC 2.4.2.30) is a nuclear enzyme found in all eukaryotes except yeast with approximately 10 6 molecules per cell. (molcells.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Biochemical reconstitution demonstrates that the mammalian complex possesses inherent E3 ubiquitin ligase activity, using Ube2H as its cognate E2. (elifesciences.org)

Results showed that with the exposure concentration increase, ROS and CCP in Hela cells presented a significant increase but GSH contents in Hela cells and cell viability showed a significant decrease respectively compared with the control. (scirp.org)

At physiological levels, ROS can improve cellular activities as they are involved in the control of the chemical balance and synaptic plasticity [ 4 ], whereas an excess amount of ROS can damage the endothelium, leading to alteration of the intracellular reduction-oxidation homeostasis [ 5 ]. (hindawi.com)
Studies performed at single-cell resolution have demonstrated the physiological significance of cell-to-cell variability. (mdpi.com)

To explore the scope of RBPs across eukaryotic evolution, we determined the in vivo RBP repertoire of the yeast Saccharomyces cerevisiae and identified 678 RBPs from yeast and additionally 729 RBPs from human hepatocytic HuH-7 cells. (nature.com)
Here we identify a large set of RBPs that are conserved between yeast and human cells. (nature.com)
Figure 1: mRNA interactome capture in yeast and HuH-7 cells. (nature.com)
a ) Schematic representation of the mRNA interactome capture protocol in yeast and HuH-7 cells using PAR crosslinking (PAR-CL) or conventional crosslinking (cCL). (nature.com)

An animation that takes the viewer inside a mammalian cell where one can see the nucleus and its characteristic envelope connected to the endoplasmic reticulum. (cellimagelibrary.org)
Additionally, expression of chimeric P-gp was able to confer a paclitaxel-resistant phenotype to HeLa cells characteristic of P-gp-mediated drug resistance. (unscburma.org)

Fauchere J.-L. , Kervella M. , Rosenau A. , Mohanna K. , Veron M. Adhesion to HeLa cells of Campylobacter jejuni and C coli outer membrane components. (microbiologyresearch.org)
Konkel M. E. , Joens L. A. Adhesion to and invasion of HEp-2 cells by Campylobacter spp. (microbiologyresearch.org)
abstract = "The physical behaviors of stationary cells, such as the morphology, motility, adhesion, anchorage, invasion and metastasis, are likely to be important for governing their biological characteristics. (edu.sa)

The proposed analysis is based on introducing perturbations to the density or distribution function of the cellular state variables at specific time points, and quantifying how such perturbations affect the state distribution at later time points. (mdpi.com)

Throughout the years, ROS and NO have been widely considered to enter cells by freely diffusing through the cell membrane lipid bilayer and not via specific transporters or channels. (hindawi.com)
This review focuses on an emerging topic, the functional involvement of AQPs in ROS membrane transport, with specific regard to the movement of hydrogen peroxide and NO into and out of cells, in both health and oxidative stress-induced diseases. (hindawi.com)
In contrast, internalisation is markedly diminished at low temperature, appears to involve active invagination of the target cell membrane via pseudopod formation, and is maximal when cells of human origin are employed. (microbiologyresearch.org)
The presence of TNF-α, IFN-γ and PAMPs in the nasal mucosa can cause RIPK3/MLKL dependent necroptosis and cell membrane rupture, which leads to the release of DAMPs such as IL-1α and ATP. (bioz.com)
This method relies on introducing a live-cell membrane-permeant DNA fluorophore, such as Hoechst 33342, into the culture medium of cells at the end of any live-cell imaging experiment and measuring each cell's integrated nuclear fluorescence to quantify DNA content. (biomedcentral.com)
To overcome the limitations brought upon by cellular fixation, membrane-permeant DNA fluorophores were designed to stain DNA stoichiometrically in live-cells (supravital staining), enabling both the quantification of DNA content and the ability to track the movement of chromosomes over time. (biomedcentral.com)

Blasticidin is an efficient selection antibiotic that acts on both eukaryotic and prokaryotic cells . (invivogen.com)

He developed the CHO cell line from Chinese hamster ovarian cells for this work. (wikipedia.org)

Am J Physiol Lung Cell Mol Physiol. (biomed.news)

Where cellular heterogeneity causes therapies to fail. (csic.es)
In this work, we present a novel sensitivity analysis method, called molecular density function perturbation (MDFP), for the dynamical analysis of cellular heterogeneity. (mdpi.com)

we obtained additional information nearly simultaneously through additional microgram-scale sampling during the respective cell assays. (biomedcentral.com)
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. (abcam.com)

Transport of NO and ROS by AQPs would be required for cell homeostasis to play a critical role in maintaining endothelial function. (hindawi.com)
PCD has fundamental functions in tissue development and homeostasis, as PCD is activated to sculpt or remove structures, regulate cell numbers, and eliminate unnecessary or dysfunctional cells. (nature.com)

Puck studied X-rays and cellular mutations. (wikipedia.org)
He also isolated and studied cellular mutations. (wikipedia.org)

Spotted cell microarrays hereafter referred to as "cell chips " are constructed by growing and treating cells under normal tissue culture conditions formaldehyde fixing and printing microsamples of each culture onto replicate glass slides. (antiviralbiologic.com)
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)

For cell cycle synchronization T98G cells were rendered quiescent by serum starvation for 48 h and then released into the cell cycle by serum stimulation. (exposed-skin-care.net)

Cell types included resting human peripheral lymphocytes, LN71 or RT2 glioma cells, Chinese-hamster-ovary cells, HeLa cells, and mouse spermatozoa. (cdc.gov)

Inducible deletion of Sphk1 or its pharmacological inhibition drive increased cell death in tumors which is accompanied by selective accumulation of sphingosine levels. (bvsalud.org)
Both Na + loading and cell swelling were dampened on inhibition of TRPV4 channels. (jneurosci.org)
1994. Inhibition of Na+-glucose cotransport in kidney cortical cells by cadmium and copper: Protection by zinc. (cdc.gov)

We developed a FluidFM-based approach to extract, inject, and transplant organelles from and into living cells with subcellular spatial resolution. (biomed.news)
We developed dedicated probes that allow minimally invasive entry into cells and optimized fluid flow to extract specific organelles. (biomed.news)
The results of this study, focused on the mechanisms of the cellular uptake, localization and secretion of UCNPs, demonstrate, for the first time, the co-localization of UCNPs within discrete cell organelles. (edu.au)

Mcsweegan E. , Buff D. H. , Walker R. I. Intestinal mucus gel and secretory antibody are barriers to Campylobacter jejuni adherence to INT 407 cells. (microbiologyresearch.org)

Our biochemical and cellular analysis thus demonstrates that the GID/CTLH complex prevents cell cycle exit in G1, at least in part by degrading Hbp1. (elifesciences.org)

Typically, mammalian cells are sensitive to blasticidin concentrations of 1-10 µg/ml, and bacteria to 25-100 µg/ml. (invivogen.com)

Specific suppression of ZIP4 reduced cell migration and invasiveness, whereas ZIP4 overexpression caused increases in cell migration and invasiveness. (ijbs.com)

L-glutamine is an essential amino acid that is a crucial component of culture media, and serves as a major energy source for cells in culture. (sigmaaldrich.com)

This work allowed the genetics of human and other mammalian cells to be studied in detail. (wikipedia.org)
S. cerevisiae ) (BY4741) and of human hepatocytic cells (HuH-7). (nature.com)
via conversation with Cdt1 in human cells. (exposed-skin-care.net)
Fauchere J. L. , Rosenau A. , Veron M. , Moyen E. N. , Richard S. , Pfister A. Association with HeLa cells of Campylobacter jejuni and Campylobacter coli isolated from human feces. (microbiologyresearch.org)
Right here we describe human spotted cell chips a technology for determining cellular state across arrays of cells subjected to chemical or genetic perturbation. (antiviralbiologic.com)
Recombinant full-length human BRSK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. (signalchem.com)

Fructosamine-3-kinase (FN3K) is involved in the deglycation of Nrf2, a significant regulator of oxidative stress in cancer cells. (bvsalud.org)
The cancer cell exhibits a larger traction force than the normal cell by ∼20% for a HeLa cell and ∼50% for a L929 cell. (edu.sa)
To achieve the above target, cancer cells preferentially use aerobic glycolysis, otherwise known as the Warburg effect, over oxidative phosphorylation. (kdwis.com)
Cancer, on the other hand, can be defined as a heterogeneous group of genetic diseases characterised by unregulated clonal expansion of somatic cells brought about by multiple genetic and epigenetic changes ( Evan and Vousden, 2001 ). (kdwis.com)
In 2000, Hanahan and Weinberg published a seminal review tagged ''the hallmarks of cancer'' ( Hanahan and Weinberg, 2000 ) which aimed at summarising the promoting features of cancer cells into 6 major hallmarks. (kdwis.com)

Thus there is a clear role for a platform that enables analysis of multiple cell types and/or treatment conditions in a manner that scales to thousands of samples while minimizing reagent cost and experimental variance. (antiviralbiologic.com)

It was found that the cellular uptake of the UCNPs occurred primarily by clathrin-mediated endocytosis, whereas they were secreted from the cells via lysosomal exocytosis. (edu.au)

Classical perturbation tests have been allowed with the creation of RNAi and chemical substance libraries but there can be found few platforms in a position to carry out cell-based experiments over the range Artemisinin of mammalian genomes particularly when multiple reporters are needed [1] [2]. (antiviralbiologic.com)

In this paper, we present a silicon-nanowire-array based technique for quantifying the mechanical behavior of single cells representing three distinct groups: normal mammalian cells, benign cells (L929), and malignant cells (HeLa). (edu.sa)

Dr. Puck with the assistance of Philip I. Marcus, successfully cloned a HeLa cell in 1955. (wikipedia.org)
GSK3 inhibitors downregulate survivin and bcl-2 via inactivation of NF-kB and successfully eliminate leukemic cells [17]. (globaltechbiz.com)

Evidence was found that direct dose or dose rate dependent alterations occur at a highly physiologically significant cellular endpoint. (cdc.gov)

Anatomy34

Organisms20

Chemicals and Drugs80

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes84

Disciplines and Occupations1

Information Science4

Health Care3

Lysate2

Reactive oxygen2

Autophagy10

Homolog2

Microscopy7

Mitochondria5

Tumor cells2

Proliferation8

Vitro9

Mitotic1

Genome2

Autophagic cell death2

Substrates3

HEK2931

Adherent1

Transiently1

Characterization1

Processes7

Lines11

Induce2

Lysates2

Transfection2

Constitutively2

Nuclei1

Chromosomal1

Induces1

Proteins6

Dynamics4

Apoptosis3

Gene8

Antibodies1

Survival6

Embryonic1

Metabolism7

Molecules4

Reconstitution1

Viability1

Physiological2

Yeast4

Characteristic2

Adhesion3

Perturbations1

Membrane6

Eukaryotic1

Ovarian cells1

Physiol1

Heterogeneity2

Assays2

Homeostasis2

Mutations2

Tissue culture6

Starvation1

Glioma1

Inhibition3

Organelles3

Antibody1

Biochemical1

Typically1

Overexpression1

Component1

Human6

Cancer5

Analysis1

Lysosomal1

Perturbation1

Distinct1

Successfully2

Evidence1