• tumor cell
  • The fine regulation of the brain-derived neurotrophic factor (BDNF) by sortilin is required for both neuronal and tumor cell survival. (wikipedia.org)
  • Furthermore, it appears that sortilin participates in the progression of breast cancer and contributes to tumor cell adhesion and invasion. (wikipedia.org)
  • Recent evidence from our laboratory and from others suggested that pretreatment with α-difluoromethylornithine (DFMO) sensitizes some human and rodent tumor cell lines to l,3-bis(2-chloroethyl)-l-nitrosourea (BCNU). (aacrjournals.org)
  • Moreover
  • Moreover, the weak effect observed on differentiated CaCo2 cells suggests a specific anticancer activity for this compound. (aacrjournals.org)
  • Moreover, prolonged drug incubation (up to 168h) resulted in massive detachment of cells from culture plates, which together with Annexin V/PI results, indicated that necrosis was the main response of HT29 cells to C-1748 treatment. (biomedsearch.com)
  • intestinal
  • The HT29-5M21 intestinal cell lineage is a mucin-secreting cellular population. (ac.be)
  • Conclusion: The culture formed by 75% Caco-2 and 25% HT29-5M21 produce a monolayer containing the two main cell types of human intestinal epithelium and characterized by a reduced permeability to macromolecules. (ac.be)
  • 3. R.Troncone, C.Gianfrani, G.Mazzarella, et al: "Majority of Gliadin-Specific T-Cell Clones from Coeliac Small Intestinal Mucosa Produce _Interferon-g and Interleukin-4" Dig Dis Sci. (cnr.it)
  • Adhesion of NCFM to mucin and intestinal HT-29 cells increased three-fold after culture with raffinose versus glucose, as also visualized by scanning electron microscopy. (dtu.dk)
  • protein
  • At the cellular level, sortilin functions in protein transport between the Golgi apparatus, endosome, lysosome, and plasma membrane, leading to its involvement in multiple biological processes such as glucose and lipid metabolism as well as neural development and cell death. (wikipedia.org)
  • In addition, two hydrophobic loops have been detected in this domain and act to anchor the protein in the cell membrane. (wikipedia.org)
  • this effect was associated with a marked reduction of doxorubicin efflux rate in HT29 and HT29-dx cells, and tyrosine nitration in the MRP3 protein. (aacrjournals.org)
  • Many human tumor cells are resistant to chloroethylnitrosourea-induced DNA interstrand cross-linking and cell kill due to their high levels of the DNA repair protein O 6 -alkylguanine DNA alkyltransferase. (aacrjournals.org)
  • Equal concentrations of BCNU produced similar levels of strand breaks and DNA-protein cross-links in DFMO-pretreated and control cultures for both cell lines. (aacrjournals.org)
  • from the Latin cingere "to form a belt around") is a cytosolic protein encoded by the CGN gene in humans localized at tight junctions (TJs) of vertebrate epithelial and endothelial cells. (wikipedia.org)
  • Embryoid bodies derived from embryonic stem cells where one or both cingulin alleles were targeted by homologous recombination show apparently normal tight junctions, but changes in the expression of a large number of genes, including tight junction protein genes (claudin-2, claudin-6, claudin-7 and occludin) and transcription factors (including GATA4). (wikipedia.org)
  • On one hand, an unknown protein induced by the stimulated Fc receptor reduces the TLRs transcription and translation, which reduce the capacity of the cell to detect viral proteins. (wikipedia.org)
  • β-catenin is a dual function protein, involved in regulation and coordination of cell-cell adhesion and gene transcription. (wikipedia.org)
  • Beta-catenin was initially discovered in the early 1990s as a component of a mammalian cell adhesion complex: a protein responsible for cytoplasmatic anchoring of cadherins. (wikipedia.org)
  • Thus beta-catenin became one of the very first examples of moonlighting: a protein performing more than one radically different cellular function. (wikipedia.org)
  • The protein interacts with the intraflagellar transport protein 81 (IFT81), the SH3-domain containing protein PRAX-1, and is phosphorylated by cyclin dependent kinase 1 ( Cdk1 ) and polo-like kinase 1 ( PLK1 ), and functions in maintaining Microtubule organization, cell morphology and cilium stability. (wikipedia.org)
  • KP418 exhibits slower cellular uptake and slower protein binding. (wikipedia.org)
  • The transferrin protein binds to the transferrin receptor and is taken into the cell by endocytosis. (wikipedia.org)
  • This protein and its receptors are overexpressed in cancer cells owing to their increased demand for iron, and it is believed that Tf transport is the reason ruthenium compounds accumulate in tumors. (wikipedia.org)
  • Alkaline phosphatase in E. coli is located in the periplasmic space and can thus be released using techniques that weaken the cell wall and release the protein. (wikipedia.org)
  • proteins
  • As a sorting receptor on the cell surface and on the endoplasmic reticulum-Golgi apparatus within the cell, sortilin is involved in the transport of a wide variety of intracellular proteins between the trans-Golgi network, endosome, lysosome, and secretory granules, as well as the plasma membrane. (wikipedia.org)
  • The secretion machinery consists of ~30 proteins which span both the inner and outer membranes of the bacterial cell. (uiowa.edu)
  • It may occur through different mechanisms, but it often correlates with the overexpression of integral membrane transporters, such as P-glycoprotein (Pgp) and MDR-associated proteins (MRPs), with resulting decrease of drug accumulation and cellular death. (aacrjournals.org)
  • In cancer cell models, one of the most studied of these mechanisms has been the overexpression of several energy-dependent drug efflux pumps that belong to the ATP-binding cassette family of transporters, such as the P-glycoprotein (Pgp) and the MDR-associated proteins (MRPs). (aacrjournals.org)
  • The overexpression of these integral membrane proteins causes tumor cells to become resistant to a variety of anticancer drugs (anthracyclines, Vinca alkaloids, epipodophyllotoxins, taxanes, actinomycin-D, mitoxantrone, etc. (aacrjournals.org)
  • Comparative proteomics using 2D-DIGE showed 43 unique proteins to change in relative abundance in whole cell lysates from NCFM grown on raffinose compared to glucose. (dtu.dk)
  • Antibody-dependent enhancement (ADE) occurs when non-neutralising antiviral proteins facilitate virus entry into host cells, leading to increased infectivity in the cells. (wikipedia.org)
  • Most of the PAR family act through the actions of G-proteins i (cAMP inhibitory), 12/13 (Rho and Ras activation) and q (calcium signalling) to cause cellular actions. (wikipedia.org)
  • Activation of PARs also led to an increase in tyrosine phosphorylation of ZAP-70 and SLP-76, two key proteins in T cell receptor (TCR) signalling. (wikipedia.org)
  • Human
  • Here, we elucidated the nature of cellular response of human colon carcinoma HCT8 and HT29 cells to C-1748 treatment, at biologically relevant concentrations (EC(90) and their multiplicity). (biomedsearch.com)
  • PURPOSE: To examine the effects of berberine, an alkaloid isolated from some medicinal herbs, on the disruption of the barrier function in a human retinal pigment epithelial cell line (ARPE-19) stimulated with interleukin-1beta (IL-1beta). (biomedsearch.com)
  • The current study was designed to investigate the anti-ROS and -RNS effects of cucurbitacin L 2-O- β -glucoside (CLG) and the role of these signaling factors in the apoptogenic effects of CLG on human colon cancer cells (HT-29). (hindawi.com)
  • Culturing the human epithelial colon cell line HT29 in the presence of doxorubicin, we obtained a doxorubicin-resistant (HT29-dx) cell population: these cells accumulated less intracellular doxorubicin, were less sensitive to the cytotoxic effects of doxorubicin and cisplatin, overexpressed Pgp and MRP3, and exhibited a lower NO production (both under basal conditions and after doxorubicin stimulation). (aacrjournals.org)
  • Therefore, we induced human colon cancer HT29 cells, a cell type very susceptible to develop a MDR phenotype (12) , to become doxorubicin-resistant. (aacrjournals.org)
  • Although cingulin has been involved in regulation of RhoA signaling and gene expression in cultured cells and KO mice, nothing is known about the specific role of cingulin in human diseases. (wikipedia.org)
  • cytotoxic
  • Multidrug resistance (MDR) is a phenomenon by which tumor cells that have been exposed to a cytotoxic agent develop cross-resistance to a range of structurally and functionally unrelated compounds (1) . (aacrjournals.org)
  • uptake
  • AS160 also contains a calmodulin-binding domain, and this domain mediates phosphorylation-independent glucose uptake in muscle cells. (wikipedia.org)
  • vitro
  • Brú's theory stems from his mathematical research on the Fractal growth of tumour cell colonies in vitro. (wikipedia.org)
  • In vitro experiments showed that the inflammatory cytokines and Type 1 IFN production was reduced when the ADE-DENV complexe bound to the Fc receptor of THP-1 cells. (wikipedia.org)
  • receptors
  • Some cells do not have the usual receptors on their surfaces that viruses use to gain entry. (wikipedia.org)
  • It has been determined that a large group of proteases cleave and activate PARs receptors, including proteases from: a) the coagulation cascade, b) inflammatory cells, and c) the digestive tract. (wikipedia.org)
  • The cellular effects of thrombin are mediated by protease-activated receptors (PARs). (wikipedia.org)
  • parental
  • In accord with mesenchymal-like morphology of 5-FU-resistant HT-29 cells, the expression of the mesenchymal marker fibronectin was significantly increased in these cells in comparision with that in the parental cells. (nih.gov)
  • Finally, 5-FU-resistant cells showed enhanced migration in comparison with parental HT-29. (nih.gov)
  • 5-FU resistant phenotype exhibits an EMT-like cellular characteristics compared with parental HT-29 cells. (nih.gov)
  • Cells with 5-FU-resistant phenotype exhibit EMT-like characteristics in comparison with the phenotype of parental HT-29 cells. (nih.gov)
  • morphology
  • Next, we demonstrated that HCT8 cells surviving after exposure to C-1748 were in the state of senescence, based on altered cell morphology and expression of a pH 6-dependent beta-galactosidase. (biomedsearch.com)
  • siRNA
  • Cells were transfected, for 4 h, with (14Ser)2N5/siRNA/HL complexes and, after an additional period of 20 h, cells were incubated with 1.5 μM ETO (C) for 48 h. (selleck.co.jp)
  • colorectal cancer
  • CD24 is a novel oncogene in colorectal cancer, detected by microarray profiling of cell transformation, and is a target for immunotherapy of cancer. (aacrjournals.org)
  • Aims:To identify genes that play a role in colorectal cancer (CRC) carcinogenesis by analysis of differential gene expression of normal and transformed CRC cell lines. (aacrjournals.org)
  • These results show that the EpCAM Apt-functionalized CUR-NPs enhance the targeting and drug delivery of CUR to colorectal cancer cells. (dovepress.com)
  • CEA was identified for the first time in 1965 in colorectal cancer ( 4 , 5 ) and has since been found in 80% to 90% of all colorectal cancers as well as in up to 50% of all breast cancers, in non-small-cell lung cancers, in esophageal, pancreatic, and gastric malignancies, as well as in malignant tumors of the thyroid and female reproductive tract ( 6 ). (aacrjournals.org)
  • concentrations
  • Reverse-phase high-performance liquid chromatography assays of polyamine concentrations in cell extracts verified that DFMO produced comparable degrees of polyamine depletion for both cell lines. (aacrjournals.org)
  • cytoskeletal
  • PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control. (wikipedia.org)
  • tumour
  • Emerging evidence suggests that reactive oxygen (ROS) and nitrogen (RNS) species can contribute to diverse signalling pathways of inflammatory and tumour cells. (hindawi.com)
  • junctions
  • Gap junctions formed by connexins (Cx) play an important role in transmitting signals between bone cells such as osteoblasts and osteoclasts, cells responsible for bone formation and bone remodeling, respectively. (biomedsearch.com)
  • Regarding the zygotic cingulin, it accumulates at the tight junctions from 16-cells stage, 10 hours after ZO-1 assembly. (wikipedia.org)
  • In Xenopus laevis embryos, maternal cingulin is recruited to apical cell-cell junctions from 2-cells stage. (wikipedia.org)
  • susceptible
  • KP1019 does not appear to be susceptible to the same cancer cell mechanism of acquiring drug resistance as other metal-based drugs, and remains potent in cell-lines known for their drug resistance. (wikipedia.org)
  • phenotype
  • We observed that the culture conditions used ( serum-free medium) did not change the phenotype of each cell type, and produced a reproducible model. (ac.be)
  • Accumulating evidence shows that transition from epithelial to mesenchymal phenotype in cancer cells is associated with their resistance to chemotherapy. (nih.gov)
  • The observed morphological changes implied that the 5-FU-resistant cells had transitioned to a mesenchymal phenotype. (nih.gov)
  • epithelium
  • Background: The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. (ac.be)
  • The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel epithelium. (ac.be)
  • vivo
  • However, there is a strong debate regarding the value of Caco-2 cell culture to mimick in vivo situation. (ac.be)
  • ADE is common in cells cultured in the laboratory, but rarely occurs in vivo except for dengue virus. (wikipedia.org)
  • regulation
  • Consistent with morphological changes, 5-FU-resistant cells showed down-regulation of the epithelial marker E-cadherin and up-regulation of the mesenchymal marker fibronectin (Fig. 2). (nih.gov)
  • drug resistance
  • We hypothesized that doxorubicin-sensitive and doxorubicin-resistant cells could show a different capacity to produce NO and that this feature might contribute to drug resistance. (aacrjournals.org)
  • stimulation
  • Insulin stimulation of fat and muscle cells results in translocation of the glucose transporter GLUT4 to the plasma membrane, and this translocation process is dependent on phosphorylation of AS160. (wikipedia.org)
  • 1995
  • 1. R.Troncone, L.Maiuri, L.Greco, et al (1995): "Mucosal Cell-Mediated Immunity In Coeliac Disease", Dyn Nutr.Res. (cnr.it)
  • alkaline
  • Results: In this study, we investigated the activity and localization of the alkaline phosphatase and the expression of IAP and MUC5AC genes to determine a correlation between these markers and the cellular composition of a differentiated monolayer obtained from a mixture of Caco-2 and HT29-5M21 cells. (ac.be)
  • The alkaline phosphatase expression characterizing Caco-2 cells was influenced by the presence of HT29-5M21 cells. (ac.be)
  • In Gram-negative bacteria, such as Escherichia coli (E. coli), alkaline phosphatase is located in the periplasmic space, external to the inner cell membrane and within the peptidoglycan portion of the cell wall. (wikipedia.org)
  • Since the periplasmic gap is more prone to environmental variation than the inner cell, alkaline phosphatase is suitably resistant to inactivation, denaturation, or degradation. (wikipedia.org)
  • As such, the main purpose of dephosphorylation by alkaline phosphatase is to increase the rate of diffusion of the molecules into the cells and inhibit them from diffusing out. (wikipedia.org)
  • Findings
  • Our findings may also imply that cellular targets for BCNU other than DNA damage may be responsible for DFMO-induced chemosensitization in the repair-proficient cells. (aacrjournals.org)
  • T3SS
  • Importantly, P. aeruginosa uses additional bacterial products encoded by the T3SS to directly deliver enzymes into cells to alter host physiology. (asmscience.org)
  • reverts
  • In particular, we were looking for: a. altered gene expression in the transformed cells that reverts to normal following exposure to a selective COX2 inhibitor of. (aacrjournals.org)
  • secretion
  • In the absence of these cues, low amounts of the type III secretion channels are assembled within the cell membranes. (uiowa.edu)
  • bacterium
  • Whole cell and surface proteomes were analyzed together with adhesive properties of the probiotic bacterium Lactobacillus acidophilus NCFM (NCFM) grown on the emerging prebiotic raffinose, exemplifying a synbiotic. (dtu.dk)
  • inflammatory
  • In these experiments, the anti-inflammatory drug Sulfasalazine increased the HSV-TK/ganciclovir bystander effect in C6, 9L and LN18 cells but not in U87 glioma cells. (biomedsearch.com)
  • lines
  • Interestingly, a significant role for sortilin has recently also been reported in the field of oncology, as it has been detected in several cancer cell lines. (wikipedia.org)