Cellular 1907 tissue culture techniques. Medical search. Frequent questions

Erdmann's work centered around the reproduction of protozoa, with a particular interest in tissue culture and in vitro cellular reproduction. (wikipedia.org)
Cell culture systems are an important in vitro tool in basic research and essential in drug discovery and development. (jneurology.com)
Since that time, cell culture methods have been greatly advanced, and the two-dimensional (2D) cell culture systems evolved to be the standard in vitro tool. (jneurology.com)
Over the last century, there has been a steady progression in the diversity and complexity of tissue culture methodologies used in biomedical research, the earliest reported examples of in vitro cell culture being that conducted by Ross G. Harrison who observed neuronal sprouting from frog embryo spinal cords on a microscope slide in 1907. (genengnews.com)
2-8 Hence the interest in generating more biologically relevant in vitro models, such as three-dimensional (3D) culture systems. (genengnews.com)
Incorporating 3D cell culture with in vitro screening processes such as high-throughput screening (HTS) and high-content screening (HCS) is necessary to identify clinically relevant compounds. (genengnews.com)
We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
It can be isolated aseptically from the bulk of maternal tissues of ovule, seed, or capsule and cultured in vitro under aseptic and controlled physical conditions to grow directly into plantlets. (cbsetuts.com)
But it is now possible to raise a hybrid plant by culturing the inviable embryos in vitro. (cbsetuts.com)
Relating in vitro to in vivo exposures with physiologically-based tissue dosimetry and tissue response models. (cdc.gov)
Radiosensitisation caused by titanium dioxide nanoparticles (TiO 2 -NPs) is investigated using phantoms (PRESAGE ® dosimeters) and in vitro using two types of cell lines, cultured human keratinocyte (HaCaT) and prostate cancer (DU145) cells. (medsci.org)
Anatase TiO 2 -NPs were synthesised, characterised and functionalised to allow dispersion in culture-medium for in vitro studies and halocarbons (PRESAGE ® chemical compositions). (medsci.org)
CAFs maintain their phenotype for numerous passages during culture in vitro without exposure to cancer cells, while NFs cannot be infinitely proliferous like cancer cells ( 10 ). (spandidos-publications.com)
Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs [3] "Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock and for the treatment of human infertility. (edu.au)
3D bioprinting techniques have brought considerable innovation in biomedicine, especially in the field of tissue engineering, allowing the production of 3D organ and tissue models for in vivo transplantation purposes or for in-depth and precise in vitro analyses. (preprints.org)

In this review, we focus on the bioactive and mechanical characteristics that natural hydrogels may possess to allow efficient production of organs and tissues for biomedical applications, emphasizing the reinforcement techniques to improve their biomechanical properties. (preprints.org)

The first cell culture experiment in history was performed in 1907 by Harrison and colleagues 1 who developed a method to directly observe the growth of a nerve fiber cell. (jneurology.com)
In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
Hanning and Harrison (1907) developed the technique. (cbsetuts.com)

We focus on understanding the biochemical, biomechanical, and cellular mechanisms underlying pathophysiology in discogenic back pain with translation from 2D to 3D and in vivo models. (osu.edu)

For physiologically relevant disease modeling, three-dimensional (3D) cell culture systems are of great importance because they provide a more representative in vivo -like micro-environment to the cells. (jneurology.com)
However, 2D cell cultures do not represent the physiological in vivo microenvironment of the cells 2 . (jneurology.com)
Usually, a homogenous cell population is cultured as a monolayer, while in vivo the cells are interacting with a heterogenous cell population. (jneurology.com)
These different 3D cell culture systems provide a more representative in vivo -like microenvironment which influences cellular features like morphology, proliferation, differentiation and migration 11,12 . (jneurology.com)
9-12 Conversely, the microenvironment generated by 3D cell culture appears more representative of that observed in vivo, resulting in relevant cell-to-cell and cell-to-extracellular matrix (ECM) signalling. (genengnews.com)
In contrast to conventional 2D methods, cells cultured in a 3D format may exhibit unique biochemical and morphological features similar to their corresponding tissues in vivo 19 (summarized in Table 1 ). (genengnews.com)
However, the concentric arrangement of heterogeneous cell populations in 3D cultures, as well as their growth pattern, mimics the initial (i) avascular stages of solid tumors in vivo , (ii) not-yet-vascularized micrometastatic foci, (iii) intercapillary tumor microregions with a high proliferative activity close to the capillaries, (iv) quiescent cells as intermediates, and (v) necrotic areas at larger distances. (genengnews.com)
An alternative technique that allows pollen tube elongation to be monitored in vivo is two photon microscopy 7 . (jove.com)

13-16 Such signalling cascades are deemed essential for a multitude of cellular processes, including differentiation and proliferation. (genengnews.com)
The objective of such a culture is to understand the control of differentiation and the nutritional requirements of such progressively developing embryos. (cbsetuts.com)
Upon an electrochemical switch, growth factor presentation was reversed, which initiated cellular differentiation along the neuronal lineages. (5dok.org)

Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)

These 2D cell culture systems have already provided many insights into basic cellular functions, biological mechanisms and various disease processes. (jneurology.com)
Among these, Raman spectroscopy has attracted increasing attention as a highly sensitive molecular imaging technique for studying complex biological systems, including cells, tissues, and various biological materials [ 4 ]. (medsci.org)
Natural-derived hydrogels, especially those obtained from the decellularization of biological tissues, are promising bioinks for 3D printing purposes, as they present the best biocompatibility characteristics. (preprints.org)

However, with this method, it is difficult to observe the morphological changes in individual pollen tubes deep inside the ovule tissue. (jove.com)

such as the loss of tissue-specific architecture, mechanical and biochemical cues, and cell-to-cell interactions. (genengnews.com)

however, researchers lack the methodology to visualize cellular behavior in such restrictive conditions. (jove.com)

as a German citizen working in biology, people began to suspect Erdmann of culturing malicious bacteria. (wikipedia.org)
Whipple pointed out the existence of great numbers of peculiar rod-shaped bacteria found in extracts of lymph node tissue and lamina propria of the intestine. (medscape.com)

Erdmann was able to successfully culture chicken bone marrow cells as part of her attempts to attenuate the cyanolophia virus. (wikipedia.org)
The field of 3D cell culture systems using diverse hiPSC-derived cells is growing and gets steadily advanced. (jneurology.com)
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
As the poly-dimethylsiloxane (PDMS) microdevices presented in this manuscript are optically transparent and air permeable, we can culture living cells inside the device and observe their growth behaviors under a microscope. (jove.com)
The technique of maintaining and growing cells, tissues, or organs, especially on artificial medium in suitable containers under controlled environmental conditions is known as tissue culture. (cbsetuts.com)
In callus culture, the explant undergoes cell division and forms an unorganized and undifferentiated mass of cells called Callus. (cbsetuts.com)
This technique allows non-destructive, label-free spectral imaging and analysis of cells, tissues, and nanoparticles [ 5 - 7 ]. (medsci.org)
Cellular morphology, time-lapse imaging, and nuclear staining demonstrated that this activity occurred in mitotically active cells. (jneurosci.org)
Tumour radiosensitivity can be modified by agents, which enhance the radiation effects in the tumour, with the objective of delivering efficient radiation doses that can eradicate cancer cells without exceeding normal tissue tolerances [ 5 ]. (medsci.org)
Cells sense and respond to the mechanics of their substrate - be it the extracellular matrix, neighboring cells or artificial matrix in cell culture. (5dok.org)

As a new type of probe, the heavy isotope of water has been widely used in the study of cell development, metabolism, tissue homeostasis, aging, and tumor heterogeneity. (medsci.org)
Physical forces are increasingly recognized as major microenvironmental cues that control tensional homeostasis in tissues. (5dok.org)

Embryo culture is useful in the multiplication of such plants e.g., makapuno nut. (cbsetuts.com)
An infection is the invasion of tissues by pathogens , their multiplication, and the reaction of host tissues to the infectious agent and the toxins they produce. (wikipedia.org)

Post-mortem brain tissue provides a valuable source to study neuroanatomy and to perform molecular and neuropathological analysis 16-18 . (jneurology.com)
This paradigm proposes that genetic and environmental factors shape a complex series of molecular and cellular interactions leading to a chronic inflammatory response. (biomedcentral.com)
I use a range of molecular biology techniques to determine the cellular function of proteins involved in the aforementioned disorders. (cardiff.ac.uk)

In addition, her work on the survival of Paramecium cultures led to important insights on cellular senescence and methods of genetic transmission. (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
Currently, the commonly used methods to quantify heavy water concentration include the density method, mass spectrometry, and spectroscopy techniques [ 3 ]. (medsci.org)
Just of the norm behind aroma Postmodernism with techniques of notable electromagnetics sent sought for the detection of details by Ludwig Boltzmann and J. methods between 11th endorsement and primary top, reflecting the voor Covenants by Rolf Landauer in the publications, have caused in defence in students and fire review. (cdseidel.de)
was detected by quantitative real-time PCR (qPCR) and culture-based methods. (cdc.gov)

The callus culture is maintained on an agar medium. (cbsetuts.com)

Surface modifications and the use of various coatings, as well as the development of the controlled release of antibiotics or proteins, have improved the osseointegration and soft-tissue integration of dental implants, as well as their antibacterial and immunomodulatory functions. (encyclopedia.pub)

A particular organ (Reproductive or somatic) like root or shoot meristems or leaf primordia or floral buds is isolated and cultured. (cbsetuts.com)

This problem can be overcome by embryo culture. (cbsetuts.com)
Embryo culture in such cases allows seedling development from most of the embryos. (cbsetuts.com)
Such embryo segments are cultured to analyze the relationship of different parts of the embryo to its final form in culture. (cbsetuts.com)

We also use functional genomics and proteomic techniques to study disease mechanisms in common, polygenic disorders including schizophrenia, Alzheimer's disease and Fuchs' endothelial corneal dystrophy (FECD). (cardiff.ac.uk)

These 3D cell culture systems range from scaffold and scaffold-free techniques to more complex systems like organoids. (jneurology.com)

We successfully detected T. whipplei in tissue biopsies with a sensitivity of 83% in untreated (5/6) and 40% in treated (4/10) cases of WD. (frontiersin.org)

Between 2010 and 2019, 16,437 participants were eligible for blood culture and 11,848 (72.1%) had a culture performed. (cdc.gov)

Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
The details of the plant tissue culture technique and its utilization are emphasized here as follows. (cbsetuts.com)

We used conjugated polymers to develop a novel neural stem cell culture substrate with anchored growth factors to promote cell self-renewal. (5dok.org)

In 1915 she was named a research associate at the Rockefeller Institute for Medical Research in Princeton, where she spent summers studying tissue culture techniques. (wikipedia.org)
1 At present, cell-based research is often performed on a variety of planar surfaces that have been modified to promote the growth of two-dimensional (2D) cellular monolayers. (genengnews.com)
Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
His current research is on wound healing, anti-cancer, anti-obesity and anti-diabetes study of several medicinal plants using animal cell culture. (iium.edu.my)

Whipple's disease (WD) is a rare chronic systemic infection with a wide range of clinical symptoms, routinely diagnosed in biopsies from the small intestine and other tissues by periodic acid-Schiff (PAS) diastase staining and immunohistological analysis with specific antibodies. (frontiersin.org)
While macrophage-induced inflammation is crucial for the response to pathogen infection, excessive or sustained inflammation can cause tissue damage and may lead to different conditions such as diabetes, cardiovascular diseases, and tumors 2 . (nature.com)
Blood cultures were processed for patients with acute fever or lower respiratory infection. (cdc.gov)

From embryonic development to tissue regeneration and disease progression, the human body is continuously subject to mechanical stresses. (5dok.org)

Here, we review the latest improvements of 3D hiPSC-based cell culture systems in the field of neuroscience. (jneurology.com)
Most of these developments have been technological improvements used to confine the beams to the targets and reduce radiation damage to the surrounding healthy tissues [ 2 ]. (medsci.org)

Cell culture is essential for a variety of applications including disease modeling, drug discovery, cell-based manufacturing and genetic engineering. (technologynetworks.com)

Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)

It is a hermeneutical practice, this act of interpretation of the culture of the 'other' so as to be understood by this, the True Culture. (atplayinthefield.com)
Gender, culture and organisational change: Putting theory into practice. (open.ac.uk)

In 1913, E. Steinhardt, C. Israeli, and R. A. Lambert grew vaccinia virus in fragments of guinea pig corneal tissue. (wikipedia.org)

One of the biggest challenges in the field of neurological disorders is the limited availability of freshly dissected human brain tissue. (jneurology.com)

He identified periodic acid-Schiff (PAS)-staining granules, most likely representing degenerating bacterial forms, within macrophages isolated from the small bowel as well as other tissue and fluid specimens (eg, pericardium, endocardium, lymph nodes, synovia, lung, brain, meninges) obtained from patients in whom Whipple disease was suspected. (medscape.com)
Alois Alzheimer might have mentioned plaques and tangles in a single short paper on pre-senile dementia in 1907, but it was the co-discover of Alzheimer's disease (AD), Oskar Fischer, who in that same year far more extensively reported neuritic plaque in 12 cases of senile dementia, a condition which he and many others refused to differentiate from Alzheimer's "pre-senile" dementia. (j-alz.com)

Recent studies suggest exosome-like nanovesicles (ELNVs) from fruits and vegetables are involved in tissue renewal process and functional regulation against inflammatory diseases or cancers. (dovepress.com)

In drug discovery and development, 3D cell culture systems are more frequently used in HTS assays 13,14 . (jneurology.com)

She worked with living animal materials at the Naples Zoological Station, and passed the university qualifying examination in 1907 which she received from the Realgymnasium in Kassel (Hessen). (wikipedia.org)
A complete necropsy was performed on each animal and tissues were fixed in 10 % neutral buffered formalin. (europa.eu)

13,24,25 Furthermore, issues are associated with the ECM component in 2D culture, which appears to be overcome in a number of 3D systems. (genengnews.com)

Skoog and Miller (1957) observed that the growth and morphogenesis were controlled by hormones, auxin (root formation), and cytokinin (shoot formation) in tissue culture. (cbsetuts.com)
In addition to temporal changes in growth factor presentation, mechanical stiffness of tissues is also dynamically altered over time. (5dok.org)

It should be noted that the cell type, as well as the 3D culture method, impacts on cell organization and formation of the 3D structure. (genengnews.com)
Various mechanical properties of the pollen tube have been studied, including turgor pressure and stiffness of the cell's apical region, which can be measured using the incipient plasmolysis method 3 , 4 and cellular force microscopy (CFM) 5 , 6 , respectively. (jove.com)
This is because the physical-chemical nature of many lignocellulosic substrates naturally lends itself to solid phase culture, and thereby represents a means to reap the acknowledged potential of this fermentation method. (mdpi.com)

Spheroids and complex organoid models allow the formation of self-assembled cell aggregates recapitulating the cellular organization and functionality of specific tissues or organs 8-10 . (jneurology.com)

The number 7 holds great significance in Western culture and is many remarkable features in number theory. (atplayinthefield.com)

In this case, young embryos are taken out from developing seeds and grown on a culture medium to form seedlings and then young plants. (cbsetuts.com)

Working in the early 1900s, Erdmann was a pioneer of cellular biology and one of few women in her field. (wikipedia.org)

Moreover, the expression of C1QBP and XDH was lower in RCC tumors compared with the tumor-associated normal tissues, and their down-regulation was associated with higher Fuhrman grade. (medsci.org)

Minimally invasive treatments allow better patient outcomes through faster recovery, shorter hospitalization periods and most importantly reduced damage to adjoining tissue. (osu.edu)

[6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)

Rachel Carson was born in western Pennsylvania in May 1907. (pophistorydig.com)

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