• retinoic acid
  • The present study has shown that the differentiating agent retinoic acid (RA) up-regulates ICAM-1 expression by melanoma cells in a dose- and time-dependent fashion. (aacrjournals.org)
  • Analysis of the distribution in the melanoma cell lines of retinoic acid receptors (RARs) showed a relationship between susceptibility to a RA-mediated increase of ICAM-1 expression and RARβ expression, suggesting that the latter receptor may play a role in the phenomenon. (aacrjournals.org)
  • Biology
  • The conference brings together leading experts from protein engineering, cell biology, cell line development and bioprocess, from both the pharmaceutical industry and academia. (sys-con.com)
  • Dr. Ince and his colleagues have created a paradigm shift, by demonstrating the ability to grow the majority of human ovarian cancers in long-term culture," said Richard J. Cote, M.D., Professor and Joseph R. Coulter Chair of the Department of Pathology, professor of biochemistry and molecular biology, Chief of Pathology at Jackson Memorial Hospital, and Director of BioNIUM. (healthcanal.com)
  • LGC Promochem: Cell Biology Collection. (wikipedia.org)
  • Hayflick is known for his research in cell biology, virus vaccine development, and mycoplasmology. (wikipedia.org)
  • tumor cells
  • Using the novel medium, his laboratory has developed 25 unique lines using ovarian cancer tumor cells from 26 patients. (healthcanal.com)
  • Hayflick's results focused attention on the finite cellular life-span was the fundamental location of age changes and that immortality was a key feature of tumor cells. (wikipedia.org)
  • mimic
  • However, cell lines are almost never "normal" as the conditions they are cultured under don't really mimic the conditions in the body, so in that case, choose one that fits your model (which ones express cellular markers that you want, or, undergo processes that you want to study? (protocol-online.org)
  • If the cultivation conditions given by the depositor differ from standard conditions used in CABRI and are not acceptable for maintenance of cultures in the long term the cell line is in addition cultivated by standard procedures for callus or suspension ( PC/1998/3/3 ) which mimic the given conditions most. (cabri.org)
  • adult
  • Dolly was the first clone produced from a cell taken from an adult mammal. (wikipedia.org)
  • Even though Dolly was not the first animal cloned, she received media attention because she was the first cloned from an adult cell. (wikipedia.org)
  • Antibiotic & Antimycotic Their embryological origin with neuroblastic cells means they can easily differentiate into neuron-like cells even though they are not considered adult neurons. (wikipedia.org)
  • differentiate
  • The cell line is pluripotent and can differentiate into cell types of all three germ layers. (wikipedia.org)
  • Since embryonal carcinoma can differentiate into cells of all three germ layers, P19 cells can also differentiate into those ectoderm, mesoderm and endoderm-like cells. (wikipedia.org)
  • while 0.5% - 1% DMSO led P19 cells differentiate to cardiac or skeletal muscle cells. (wikipedia.org)
  • Other than into neurons and astrocytes, P19 cells can also differentiate to oligodendrocytes, which can be detected using the specific markers, myelin-associated glycoprotein and 2',3'-Cyclic-nucleotide 3'-phosphodiesterase. (wikipedia.org)
  • PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor or dexamethasone. (wikipedia.org)
  • growth
  • Motility response to insulin-like growth factor-I (IGF-I) in MCF-7 cells is associated with IRS-2 activation and integrin expression. (protocol-online.org)
  • Is the amount of NaHCO3 improper for the growth of MCF-7 cells? (protocol-online.org)
  • If the media you're using is phenol red free, this also reduces the cell growth rate, because the chemical structure of phenol red is similar to estrogen, and the cells respond accordingly. (protocol-online.org)
  • RESULTS: There were observable differences in growth of the cells on laboratory plastic and collagen matrix. (biomedsearch.com)
  • The endothelial growth factor antibodies used did not inhibit or stimulate cell growth when compared to control but did discourage vascular mimicry. (biomedsearch.com)
  • R.U. Schenk and A.C. Hildebrandt, Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures, Can. (springer.com)
  • P19 cells can be maintained exponential growth because of a stable chromosomal composition. (wikipedia.org)
  • Treatment of PC12 cells with nerve growth factor creates cells with long processes known as neurite varicosities, which contain small amounts of vesicles. (wikipedia.org)
  • PC12 cells treated for 10-14 days with nerve growth factor had no release of vesicles from the cell body which indicates the aggregation of vesicles in the ends of the neurites. (wikipedia.org)
  • mice
  • Moreover, oligodendrocytes also developed and migrated into fiber bundles in mice when the RA-induced cells were transplanted into the brains. (wikipedia.org)
  • human
  • Speaker Alison Porter , Head of Cell Line Development in the Mammalian Cell Culture group at Fujifilm Diosynth Biotechnologies (FFDB) clarifies how ''With cell line construction on the critical path for first in human studies, it is vital to have optimised the early stages of cell line development. (sys-con.com)
  • The CD10 enzyme is a key player to identify and regulate human mammary stem cells. (currentprotocols.com)
  • Thus tumor cell lines derived using this new methodology represent a significantly improved new platform to study human tumor pathophysiology and response to therapy. (healthcanal.com)
  • THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. (wikipedia.org)
  • Cell line of human origin. (wikipedia.org)
  • In 1962 he discovered that, contrary to the belief prevalent at the turn of the century, cultured normal human and animal cells have a limited capacity for replication. (wikipedia.org)
  • Hayflick developed the first normal human diploid cell strains for studies on human aging and for research use throughout the world. (wikipedia.org)
  • determined that Saos-2 cells "possess several osteoblastic features and could be useful as a permanent line of human osteoblast-like cells and as a source of bone-related molecules. (wikipedia.org)
  • MTNR1B mRNA is expressed in human islets, and immunocytochemistry confirms that it is primarily localized in beta cells in islets. (wikipedia.org)
  • serum
  • Probably do a negative control if you have to include serum, i.e. cells without treatment. (protocol-online.org)
  • Because they are unable to support living cells on their own, they are supplemented with tissue extracts, feeder layers, anti-apoptic drugs and serum. (healthcanal.com)
  • Dolly
  • The cell used as the donor for the cloning of Dolly was taken from a mammary gland, and the production of a healthy clone therefore proved that a cell taken from a specific part of the body could recreate a whole individual. (wikipedia.org)
  • mRNA
  • The presence of angiotensinogen messenger RNA (mRNA) was assessed in total RNA extracted from hepatoma, glioma, neuroblastoma, and glioma-neuroblastoma hybrid cell lines. (ahajournals.org)
  • Dexamethasone treatment of the hepatoma cells resulted in a large increase in angiotensinogen mRNA, whereas estradiol had no effect. (ahajournals.org)
  • Steroids failed to induce detectable levels of angiotensinogen mRNA in total RNA from the other cell lines. (ahajournals.org)
  • Actin mRNA sequences were detected in all cell lines. (ahajournals.org)
  • Blot hybridization of poly(A)+RNA resulted in the visualization of a weak angiotensinogen mRNA signal for a glioma cell line and a glioma-neuroblastoma hybrid line. (ahajournals.org)
  • However, the ability to detect angiotensinogen mRNA in a cell may depend on the phenotype expressed, which can be governed by culture conditions. (ahajournals.org)
  • The enhancement of ICAM-1 cell surface expression is paralleled by an increase in ICAM-1 mRNA. (aacrjournals.org)
  • ATCC
  • Because some papers signed and I think it still grew well and formed domes as ATCC indicated!Neverthless, its doubling time was not as fast as ATCC indicated.Is DMEM not suitable for MCF-7 cells? (protocol-online.org)
  • Therefore, should I followed ATCC to add insulin to culture MCF-7 cells? (protocol-online.org)
  • cancer
  • CD10: A tool to crack the role of stem cells in breast cancer. (currentprotocols.com)
  • Ince also sent the cell lines to researchers at several top cancer research institutions, who became co-authors of the journal article, and their findings matched those attained in Ince's laboratory. (healthcanal.com)
  • These cell lines are incredibly valuable because they allow researchers in the field to study the amazing diversity that is routinely seen in each patient's cancer. (healthcanal.com)
  • We have previously identified the differential expression of this gene in rat cancer cell lines with different cancer phenotypes (3). (aacrjournals.org)
  • However, researchers utilize these cells to study the development of cancer cells in order to find more specific treatments. (wikipedia.org)
  • Proc
  • B.G. Gengenbach, C.E. Green and C.M. Donovan, Inheritance of selected pathotoxin resistance in maize plants regenerated from cell cultures, Proc. (springer.com)
  • callus
  • M. Behnke, Selection of potato callus for resistance to culture filtrates of Phytophthora infestans and regeneration of resistant plants, Theor. (springer.com)
  • patient's
  • The technique offers hope for quickly advancing precision medicine applications for a variety of cancers by enabling clinicians to test therapies on cells from a patient's own tumor in the laboratory, thus identifying the most targeted therapy for that patient. (healthcanal.com)
  • centre
  • When the final quality check and authentication have been carried out and the cultivation conditions are acceptable for the collection centre the cell line is finally accepted and included into the public collection. (cabri.org)
  • protein
  • After 10 days of exposure, astroglial cells can be detected using glial fibrillary acidic protein (GFAP), which is a specific marker of glial cells. (wikipedia.org)
  • It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interaction, and immunohistochemistry. (wikipedia.org)
  • characteristics
  • i read from some journals the type of cell that usually been used, but what the characteristics that is important to look at? (protocol-online.org)
  • The key to Ince's research is his development of a mixture of essential nutrients in liquid form, commonly referred to as a "cell culture medium," that permits cells taken from a patient tumor to replicate in a Petri dish and become a permanent cell line faithful to the characteristics of the original tumor over a long period of time in the laboratory. (healthcanal.com)
  • vesicles
  • The vesicles hold catecholamines, mostly dopamine but also limited amount of norepinephrine, and release of these neurotransmitters give rise to spikes due to changes in current similar to chromaffin cells. (wikipedia.org)
  • differential
  • The five melanoma cell lines tested displayed a differential susceptibility to the modulation of ICAM-1 expression by RA, since the cell line MeWo did not change in its ICAM-1 expression following incubation with RA. (aacrjournals.org)
  • novel
  • Further feedback from over 50 industry professionals and academics also highlight additional trends such as high throughput screening of cell lines, Quality by Design applications , cell line development strategies for novel products, analytical tools for product evaluation and bioinformatics applications . (sys-con.com)
  • molecular
  • Only a rare minority of cancers are able to grow as cell lines in these media, and when they do, they are often very different at the molecular level from the tumor of origin. (healthcanal.com)
  • Cloning in biotechnology refers to processes used to create copies of DNA fragments (molecular cloning), cells (cell cloning), or organisms (organism cloning). (wikipedia.org)