Cells from mice nk cell lectin like receptor subfamily k. Medical search. Frequent questions

Binding of the CD94/NKG2A receptor by its ligand (Qa-1(b)) did not significantly inhibit CD8(+) T cell effector functions. (duke.edu)
The NKG2D-ligand interaction triggers an activating signal in the cell expressing NKG2D and this promotes cytotoxic lysis of the cell expressing the ligand. (ox.ac.uk)
Most normal tissues do not express ligands for NKG2D, but ligand expression has been documented in tumour and virus-infected cells, leading to lysis of these cells. (ox.ac.uk)
NKG2D-RAE-1 receptor-ligand variation does not account for the NK cell defect in nonobese diabetic mice. (ox.ac.uk)
NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand. (zxbiopharm.com)
ACKR3 binds the chemokine CXCL12 (stromal cell-derived factor 1, SDF-1 which is also a ligand for CXCR4). (guidetomalariapharmacology.org)
The ligand binding of this receptor leads to the activation of multiple downstreamsignaling molecules, including JAK1, JAK3, STAT1, and STAT3. (creativebiomart.net)

The expression of CD94/NKG2A was rapidly initiated in naive CD8(+) T cells responding to peptide Ags in vitro and on many of the naive T cells that proliferate when transferred into lymphopenic (Rag-1(-/-)) hosts. (duke.edu)
Here we show that TGF-beta regulates proliferation, migration, and tumorigenicity of mesenchymal GBM cancer stem cells (CSCs) in vivo and in vitro. (uni-regensburg.de)
On a functional level, proneural CSC lines caused a significantly stronger TGF-beta-dependent suppression of NKG2D expression on CD8(+) T and NK cells in vitro providing a mechanistic explanation for the reduced immune infiltration of proneural GBMs. (uni-regensburg.de)
Bennett IM, Zatsepina O, Zamai L, Azzoni L, Mikheeva T, Perussia B. Definition of a natural killer NKR-P1A+/CD56-/CD16- functionally immature human NK cell subset that differentiates in vitro in the presence of interleukin 12. (bdbiosciences.com)
In this study, we show that segregation of the NK-mediated killing phenotype did not correlate with the NOD Raet1 haplotype and that the large alterations in NKG2D expression previously reported on NK cells expanded in vitro were not observed in primary, poly(I:C)-elicited NK cells in vivo. (ox.ac.uk)
Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. (rndsystems.com)
Since its discovery in 2000, initial in vitro studies have found that the engagement of Siglec-8 with a monoclonal antibody or with selective polyvalent sialoglycan ligands induced the cell death of eosinophils and inhibited mast cell degranulation. (mdpi.com)
Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
Applications Reported: PK136 has been reported for use in flow cytometric analysis, depletion of NK cells, and in vitro functional studies. (thermofisher.com)
Cells grown in vitro from neoplastic tissue. (lookformedical.com)

In vivo, a substantially increased infiltration of immune cells was observed in mesenchymal GBMs, while immune infiltrates were rare in proneural GBMs. (uni-regensburg.de)
NK cells from NOD mice induced with poly(I:C) in vivo exhibit low cytotoxicity against a range of target cells, but the genetic mechanisms controlling this defect are yet to be elucidated. (ox.ac.uk)
However, little is known about how T Reg suppress T cell activation in vivo. (dukecancerinstitute.org)
Dendritic cells carrying the mutation exhibited defective interstitial migration in vivo. (life-science-alliance.org)

Thus, CD94/NKG2A expression is a common consequence of CD8(+) T cell activation. (duke.edu)
However, expression of CD94 and NKG2A transgenes partially inhibited early events of T cell activation. (duke.edu)
NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. (rndsystems.com)
KLRG1 inhibits cell activation and proliferation, and is a marker of T cell senescence. (biolegend.com)
Inhibits cell activation and proliferation, marker of T cell senescence. (biolegend.com)
NKG2A functions intrinsically within ECTV-specific CD8 + T cells to limit excessive activation, prevent apoptosis, and preserve the specific CD8 + T cell response. (wustl.edu)
A role for the src family kinase Fyn in NK cell activation and the formation of the repertoire of Ly49 receptors. (unil.ch)
MLKL activation also leads to the protease, ADAMs-mediated ectodomain shedding of cell surface proteins of necroptotic cells. (biomedcentral.com)
It is likely that polyclonal B-cell lymphoproliferation in AIDS is a complex result of EBV infection, HIV antigenic stimulation, and T-cell-dependent HIV activation. (lookformedical.com)
transiting of such constituent c receptor type VIa regions in kinase during activation. (evakoch.com)
The key role in the pathogenesis of sarcoidosis is played by proinflammatory cytokines and chemokines, molecules crucially involved in the activation of immune and inflammatory cells and their trafficking to the site of disease [4]. (thebiotechdictionary.com)

The DX12 monoclonal antibody specifically binds to human CD161 which is also known as Natural killer cell surface protein P1A (NKR-P1A). (bdbiosciences.com)
The DX12 antibody can reportedly inhibit spontaneous cytotoxicity mediated by certain NK cell clones. (bdbiosciences.com)
The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation. (rndsystems.com)
Phycoerythrin conjugated Mouse anti Rat CD161 antibody, clone 10/78 ( MCA1427PE ) used for the identification of NK cells in rat blood samples by flow cytometry. (bio-rad-antibodies.com)
A: 3-week-old (young) Lewis rats were injected twice with a NK cell depleting antibody (3.2.3). (bio-rad-antibodies.com)
The mouse monoclonal antibody 1D11 recognizes an extracellular epitope of CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells. (exbio.cz)
Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD314 (1D11) APC antibody. (exbio.cz)
Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. (thermofisher.com)
For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. (thermofisher.com)
Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at less than or equal to 0.5 µg per test. (thermofisher.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)

Regulation of ligands for the activating receptor NKG2D. (ox.ac.uk)
In both humans and mice, multiple different genes encode ligands for NKG2D, and these ligands are non-classical major histocompatibility complex class I molecules. (ox.ac.uk)
Defects in the expression of NKG2D and its ligands, the RAE-1 molecules, have been hypothesized to contribute to the reduced NK function present in NOD mice. (ox.ac.uk)

Killer cell lectin-like receptor subfamily A (KLRA, alternative nomenclature Ly49) is a gene cluster coding proteins from family Ly49, which are membrane receptors expressed mainly on the surface of NK cells and other cells of immune system in some mammals including rodents and cattle but not humans. (wikipedia.org)
Mouse Klra gene cluster is located on chromosome 6 and comprises 20-30 genes and pseudogenes, e.g. (wikipedia.org)
Klra gene family is highly polymorphic and polygenic and various mouse strains encode different number of Klra genes. (wikipedia.org)
CD161 is encoded by the KLRB1 (Killer cell lectin-like receptor subfamily B member 1) gene. (bdbiosciences.com)
NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. (rndsystems.com)
The majority of mantle-cell lymphomas are associated with a t(11;14) translocation resulting in overexpression of the CYCLIN D1 gene (GENES, BCL-1). (lookformedical.com)
The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). (creativebiomart.net)
Knockout studies of a similar gene in mouse suggesta role for this gene in regulating immunoglobulin production. (creativebiomart.net)
The Rhodopsin family accounts for ~68% of the Ciona GPCR repertoire wherein the LGR-like subfamily exhibits a lineage specific gene expansion of a group of receptors that possess a novel domain organisation hitherto unobserved in metazoan genomes. (biomedcentral.com)

Natural Killer group 2D (NKG2D) has recently emerged as a major activating receptor on T lymphocytes and natural killer cells. (ox.ac.uk)
Results: Tregs depletion enhanced the frequency of specific IFNÎ³+ T cells (Th1 lymphocytes) and cytokine production after either the first or second vaccine dose. (bvsalud.org)
Human peripheral blood lymphocytes were stained with CD56 APC and KLRG1 (clone SA231A2) FITC (top) or mouse IgG2a, κ FITC isotype control (bottom). (biolegend.com)
Human peripheral blood lymphocytes were stained with CD56 APC and KLRG1 (clone SA231A2) PE/Dazzle™ 594 (top) or mouse IgG2a, κ PE/Dazzle™ 594 isotype control (bottom). (biolegend.com)
NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161, cluster of differentiation 161), refers to Natural Killer (NK) cells, lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. (zxbiopharm.com)
A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes. (lookformedical.com)
They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation. (lookformedical.com)
A form of non-Hodgkin lymphoma having a usually diffuse pattern with both small and medium lymphocytes and small cleaved cells. (lookformedical.com)
A classification of B-lymphocytes based on structurally or functionally different populations of cells. (lookformedical.com)
GM-CSF down-regulates inhibited by factors( T-lymphocytes, mouse heterotrimers, necessary phosphatidylinositol, p46 acids) estimated at acids of intrinsic tissues. (evakoch.com)

NKG2A, which is encoded by Klrc1, is a lectin-like inhibitory receptor that is expressed as a heterodimer with CD94 on NK cells and activated CD8 + T cells. (wustl.edu)
Finally, we showed the sE-cadherin/KLRG1 inhibitory receptor is the major pathway for necroptosis-mediated suppression of the anti-tumor activity of T cells and the promotion of metastasis. (biomedcentral.com)

For death-receptor-induced necroptosis, the protein kinase receptor-interacting protein kinase 1, 3 (RIPK1, RIPK3) and mixed lineage kinase domain-like protein (MLKL) are the key components of the necroptosis machinery [ 1 , 2 , 3 ]. (biomedcentral.com)

Additional studies indicate a complex genetic control of defective NOD NK cells including genes linked to the MHC and possibly those that are associated with an altered cytokine response to the TLR3-agonist poly(I:C). (ox.ac.uk)
Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. (zxbiopharm.com)
Genes down-regulated in comparison of B cells versus NK cells. (gsea-msigdb.org)
This is evident at the subfamily level comparisons since Ciona GPCR sequences are significantly analogous to vertebrate GPCR subfamilies even while exhibiting Ciona specific genes. (biomedcentral.com)

In addition, we are investigating the role of innate immune cells such as natural killer (NK) cells in innate and adaptive immune responses to these viruses. (dukecancerinstitute.org)
To elicit innate and adaptive immune responses, inflammatory cells must efficiently migrate through the complex, physiological environments in the body. (life-science-alliance.org)

G protein-coupled receptors (GPCRs) constitute a large family of integral transmembrane receptor proteins that play a central role in signal transduction in eukaryotes. (biomedcentral.com)

A specific PLEKHA4 siRNA was transfected in glioma U251 and T98G cells, and the changes in cell proliferation ability were assessed by real-time cell analysis technology and Edu experiment. (bvsalud.org)
This receptor transduces thegrowth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells,and natural killer (NK) cells. (creativebiomart.net)
Measured by its ability to inhibit IL-21-dependent proliferation of N1186 human T cells. (creativebiomart.net)

KLRC3 has also been shown to have interactions with KLRD1, TYROBP, HLA-A, and HLA-C in pathways such as the antigen processing and natural killer cell mediated cytotoxicity pathways. (novusbio.com)

CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). (exbio.cz)

However, the complement C5a receptor 2 may act as a decoy receptor for C5a, as it has no reported G protein signalling capacity. (guidetomalariapharmacology.org)
complement C5a receptor 1 [Sour. (gsea-msigdb.org)

Wu J, Groh V, Spies T: T cell antigen receptor engagement and specificity in the recognition of stress-inducible MHC class I-related chains by human epithelial gamma delta T cells. (exbio.cz)

conversely, very few Ag-specific T cells expressed Ly49 family members. (duke.edu)

Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells. (thermofisher.com)

Klra MGI Mouse Complex/Cluster/Region Detail - MGI:96876 - killer cell lectin-like receptor, subfamily A". www.informatics.jax.org. (wikipedia.org)
Because natural killer T (NKT) cells have been implicated in these diseases, we asked if these cells were affected by DOCK8 deficiency. (ox.ac.uk)
Killer cell lectin-like receptor subfamily G member 1 (KLRG1) is a 30 kD, type II membrane glycoprotein with one C-type lectin domain and one immunoreceptor tyrosine-based inhibititory motif (ITIM). (biolegend.com)

The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. (zxbiopharm.com)

cytochrome P450 family 51 subfamily A me. (gsea-msigdb.org)

Supplementary MaterialsCorrelation network analysis reveals relationships between microRNAs, transcription factor and deregulated cytokine/chemokine-receptor network in pulmonary sarcoidosis 121378. (thebiotechdictionary.com)
C-X-C motif chemokine receptor 4 [Source. (gsea-msigdb.org)

adiponectin receptor 2 [Source:HGNC Symb. (gsea-msigdb.org)

CD161 is expressed mostly on NK cell populations and on subsets of CD4+ and CD8+ αβ T cells, NKT cells and γδ T cells. (bdbiosciences.com)
Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. (exbio.cz)

KLRG1 is expressed by subsets of NK, effector and memory T cells. (biolegend.com)
Subsets of NK, effector and memory T cells. (biolegend.com)

Necroptosis is a form of regulated necrosis and is executed by MLKL when MLKL is engaged in triggering the rupture of cell plasma membrane. (biomedcentral.com)
As a necrotic cell death, the rupture of cell plasma membrane of necroptotic cells results in the release of many cellular factors of necroptotic cells, which may trigger inflammation and immune responses [ 17 , 18 ]. (biomedcentral.com)

Binding of KLRG1 to E-, N-, or R- cadherins blocks phosphorylation of Akt and increases the expression of cell cycle inhibitors. (biolegend.com)

CD161 is a member of the C-type lectin superfamily and is also referred to as C-type lectin domain family 5 member B (CLEC5B). (bdbiosciences.com)

NK cells express several families of receptors that play central roles in target cell recognition. (duke.edu)
We have been interested in the role of pattern-recognition receptors including Toll-like receptors (TLRs)in innate immune recognition of these viruses as well as their signaling pathways. (dukecancerinstitute.org)
KLRC3 codes for a protein that works as a receptor for the recognition of MHC molecules by natural killers, and in some cases cytotoxic T-cells. (novusbio.com)
ALOX5 arachidonate 5-lipoxygenase: macrophages infected with Candida albicans, Aspergillus flavus or Aspergillus fumigatus or treated with Curdlan, a selective agonist of pattern recognition receptor for fungi Dectin-1, displays increased expression of ALOX5, ALOX15 and PTGS2 Karnam et al, 2015 . (polygenicpathways.co.uk)

Adenosine exerts anti-inflammatory effects on a number of immune cells types. (guidetomalariapharmacology.org)

Nearly 39% of Ciona GPCRs have unambiguous orthologs of vertebrate GPCR families, as defined for the human, mouse, puffer fish and chicken genomes. (biomedcentral.com)

These cells can participate in inflammatory responses by releasing mediators that attract or activate other cells, contributing to the pathogenesis of allergic and non-allergic diseases. (mdpi.com)

The outcome of an encounter between a cytotoxic cell and a potential target cell depends on the balance of signals from inhibitory and activating receptors. (ox.ac.uk)

To determine how the profile of NK receptor expression changes in murine CD8(+) T cells as they respond to intracellular pathogens, we used class I tetramer reagents to directly examine Ag-specific T cells during lymphocytic choriomeningitis virus and Listeria monocytogenes infections. (duke.edu)

These subtle effects suggest that CD94/NKG2A-mediated inhibition of T cells may be limited to particular circumstances or may synergize with other receptors that are similarly up-regulated. (duke.edu)
Here, we generated Klrc1 -/- mice and found that NKG2A is selectively required for resistance to ectromelia virus (ECTV). (wustl.edu)
Thus, although inhibitory receptors often cause T cell exhaustion and viral spreading during chronic viral infections, NKG2A optimizes CD8 + T cell responses during an acute poxvirus infection. (wustl.edu)

Scholars@Duke publication: Viral and bacterial infections induce expression of multiple NK cell receptors in responding CD8(+) T cells. (duke.edu)

These NK cell receptors are also expressed by certain memory phenotype CD8(+) T cells, and in some cases are up-regulated in T cells responding to viral infection. (duke.edu)

Reports indicate that CD161 is expressed preferentially on CD45RO+ T cells, however, it can be found on a subset of thymocytes and fetal liver T cells. (bdbiosciences.com)
Detects mouse CD161/NK1.1 in Flow Cytometry. (rndsystems.com)
Figure B. Alexa Fluor 647 conjugated Mouse anti Rat CD54 ( MCA773A647 ) and FITC conjugated Mouse anti Rat CD161 ( MCA1427F ). (bio-rad-antibodies.com)
Intraperitoneal injection of 3.2.3 lead to complete absence of CD161+ NK/NKT cells and did not affect cell numbers of CD3+CD161- T cells. (bio-rad-antibodies.com)

Figure A. Alexa Fluor 647 conjugated Mouse anti Rat CD54 ( MCA773A647 ) and FITC conjugated Mouse IgG1 isotype control ( MCA1209F ). (bio-rad-antibodies.com)

Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. (thermofisher.com)

abstract = "CD8+ T cells and NK cells protect from viral infections by killing virally infected cells and secreting interferon-γ. (wustl.edu)

Blood was taken and analyzed by flow cytometry for the presence of CD3+ and CD161+ cells. (bio-rad-antibodies.com)
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10 6 cells in a suspension. (exbio.cz)
The colony-forming ability of the cells was evaluated using plate cloning assay, and cell cycle changes and cell apoptosis were analyzed with flow cytometry. (bvsalud.org)

BST2 bone marrow stromal cell antigen 2 (Tetherin): Candida albicans enhanced the production of the CCR5-interacting chemokines CCL3, CCL4, and CCL5, and stimulates the production of interferon-a and the restriction factors APOBEC3G, APOBEC3F, and tetherin (BST2) in macrophages Rodriguez et al, 2013 . (polygenicpathways.co.uk)

Methods: We evaluated the effect of Tregs depletion on the immunogenicity of an experimental recombinant anti-Sporothrix vaccine, using the DEREG mice. (bvsalud.org)
In this model, only Foxp3(+) Tregs express eGFP and diphtheria toxin (DT) receptors, and transient Tregs depletion is achieved by DT administration. (bvsalud.org)
Importantly, the increased anti-tumor activity of T cells is a key cause for the reduced metastasis as the depletion of CD8+ T cells completely restored the level of metastasis in the Mlkl KO mice. (biomedcentral.com)

The immunization with peptide mixture (PeptMix) formulated with Freund adjuvant (FA), induced high levels of antigen-specific IgG, IgG1 and IgG2b antibodies that conferred protection upon passive transference in infected BALB/c mice with S. brasiliensis. (bvsalud.org)
formyl peptide receptor 2 [Sour. (gsea-msigdb.org)

In the thymus, DOCK8-deficient mice lack a terminally differentiated subset of NK1.1(+) NKT cells expressing the integrin CD103, whereas in the liver, DOCK8-deficient NKT cells express reduced levels of the prosurvival factor B-cell lymphoma 2 and the integrin lymphocyte function-associated antigen 1. (ox.ac.uk)
1 million mouse splenocytes were stained with 0.125µg NK1.1-PE. (zxbiopharm.com)

Full documentation can be found in the GtoImmuPdb immuno cell type data documentation (PDF) . (guidetomalariapharmacology.org)
It belongs to the type Icytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, areceptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. (creativebiomart.net)
GABA type A receptor associated. (gsea-msigdb.org)

Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. (lookformedical.com)

Siglecs (sialic acid-binding immunoglobulin-like lectins) are single-pass cell surface receptors that have inhibitory activities on immune cells. (mdpi.com)

Clinically, EBV-associated malignancies such as Hodgkin's lymphoma offer a unique model to explore antigen-defined immunotherapy approaches because EBV-derived tumor antigens are specific for tumor cells only. (dukecancerinstitute.org)

B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. (lookformedical.com)

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