• Objective: Diabetic kidney disease (DKD) is a severe kidney disease characterized by podocyte apoptosis, injury, and accumulation of extracellular matrices that ultimately lead to end-stage renal disease. (ijsciences.com)
  • Results: Compared with the NG group, the expression of apoptosis proteins Caspase-3 and Bax in the podocytes of HG group increased, and the expression of Bcl-2 decreased. (ijsciences.com)
  • Conclusion: Vitamin D can alleviate the apoptosis of podocytes induced by high glucose in vitro. (ijsciences.com)
  • Astragaloside IV prevents high glucose-induced podocyte apoptosis via downregulation of TRPC6[J]. Molecular Medicine Reports, 2016, 13(6):5149. (ijsciences.com)
  • High glucose-induced apoptosis in cultured podocytes involves TRPC6-dependent calcium entry via the RhoA/ROCK pathway[J]. Biochemical & Biophysical Research Communications, 2013, 434(2):394-400. (ijsciences.com)
  • Astragaloside IV, a novel antioxidant, prevents glucose-induced podocyte apoptosis in vitro and in vivo[J]. Plos One, 2012, 7(6):e39824. (ijsciences.com)
  • 1,25-Dihydroxyvitamin D3 Prevents Puromycin Aminonucleoside-Induced Apoptosis of Glomerular Podocytes by Activating the Phosphatidylinositol 3-Kinase/Akt-Signaling Pathway[J]. American Journal of Nephrology, 2009, 30(1):34-43. (ijsciences.com)
  • High glucose induces podocyte apoptosis by stimulating TRPC6 via elevation of reactive oxygen species. (ijsciences.com)
  • β-Arrestin 1/2 Aggravates Podocyte Apoptosis of Diabetic Nephropathy via Wnt/β-Catenin Pathway[J]. Med Sci Monit, 2018, 24:1724-1732. (ijsciences.com)
  • Glucose-induced reactive oxygen species cause apoptosis of podocytes and podocyte depletion at the onset of diabetic nephropathy. (ijsciences.com)
  • Podocyte hypertrophy precedes apoptosis under experimental diabetic conditions[J]. Apoptosis, 2015, 20(8):1056-1071. (ijsciences.com)
  • In general though, endothelin can elicit a wide range of pathophysiological effects leading to things like cell proliferation, apoptosis, inflammation and fibrosis. (medtelligence.net)
  • Akt pathways have effects on apoptosis, protein synthesis, metabolism and cell cycle. (springer.com)
  • Changes in glycemia and the rate of glycolysis can also lead to a reduction in the formation of foot processes by podocytes, a decrease in their migratory ability, and the induction of apoptosis. (encyclopedia.pub)
  • In mice with streptozotocin-induced type 1 diabetes, Klotho treatment suppressed cardiac inflammation, lowered oxidative stress, and prevented cardiac cell apoptosis [ 20 ] . (encyclopedia.pub)
  • Although the senescent cells remain viable, they show typical changes with enlarged and flattened cell bodies, apoptosis resistance, increased activity of senescence-associated β -galactosidase (SA- β -gal), and upregulation of cyclin-dependent kinase (CDK) inhibitors including p16 INK4A , ARF proteins, and p21 [ 13 - 16 ]. (hindawi.com)
  • QLα12 LacZ+/Cre+ mice showed no changes in podocyte number, apoptosis, proliferation or Rho/Src activation. (medscape.com)
  • It was also demonstrated that USF1 directly binds to the promoter region of 2,492 genes, which may be involved in the viral progression and cell proliferation pathways. (spandidos-publications.com)
  • Endothelin also directly stimulates mesangial cell proliferation, contraction, and extracellular matrix production-effects that are important in the pathophysiology of IgAN. (medtelligence.net)
  • Hepatocyte growth factor (HGF) is produced by stromal and mesenchymal cells, and it stimulates epithelial cell proliferation, motility, morphogenesis and angiogenesis in various organs via tyrosine phosphorylation of its cognate receptor, Met. (spandidos-publications.com)
  • Aside from its hypertensinogenic effect, locally produced Ang II in the kidney activates multiple intracellular signaling pathways and induces cell proliferation and tissue remodeling by enhancing the synthesis of profibrotic cytokines and chemokines ( 3 4 ). (koreamed.org)
  • MAPK pathways refer to various cellular functions, including cell proliferation, differentiation and migration. (springer.com)
  • It has subsequently been found to be located in the glomerular epithelial cell, i.e., the podocyte. (renalandurologynews.com)
  • Electron microscopy typically shows fusion of epithelial cell foot processes, or so-called foot process effacement. (biomedcentral.com)
  • a combination of phospholipase A2 receptor antibody and corresponding antigen on the podocytes forms in the immune complex and then activates the C5b-9 complex through the relative channels, damages the podocytes, destroys the glomerular filtration barrier, and generates proteinuria [ 5 - 7 ]. (hindawi.com)
  • Neutral endopeptidase, a podocyte antigen that can digest biologically active peptides, was identified as the target antigen in a subset of patients with antenatal membranous nephropathy. (medscape.com)
  • This antigen/antibody complex stimulates the formation of new basement membrane tissue that grows up and around the antigen-antibody complex deposits and creates thickening of the GBM when viewed down the microscope. (renalandurologynews.com)
  • The glomerular capillaries are lined by a fenestrated endothelium that sits on the glomerular basement membrane, which in turn is covered by glomerular epithelium, or podocytes, which envelops the capillaries with cellular extensions called foot processes. (medscape.com)
  • In these mice, podocyte foot processes were also more sparse and disperse than in wild-type mice. (wikipedia.org)
  • We extracted CD4+ T cells from SLE-prone Fcgr2b-/- mice to elaborate the mechanism of mitochondrial Lon protease in CD4+ T cell activation in SLE. (bvsalud.org)
  • It was demonstrated that STING expression was elevated in CD4+ T cells in SLE-prone Fcgr2b-/- mice. (bvsalud.org)
  • The data indicated that STING regulated IFN signaling to promote CD4+ T cell activation in SLE-prone Fcgr2b-/- mice. (bvsalud.org)
  • Moreover, the roles of cGAS, STING, and TBK1 in activating CD4+ T cells from SLE-prone Fcgr2b-/- mice were evaluated using gain- or loss-of-function experiments. (bvsalud.org)
  • Besides, cytosolic mtDNA could activate CD4+ T cell activation in SLE-prone Fcgr2b-/- mice by upregulating the cGAS-STING-TBK1 axis. (bvsalud.org)
  • The function of mitochondrial Lon protease in oxidative damage and mtDNA release in CD4+ T cells of SLE-prone Fcgr2b-/- mice were explored. (bvsalud.org)
  • We hypothesized that NOX4-derived H2O2 contributes to podocyte damage in DKD via elevation of podocyte calcium.Methods We used Dahl salt-sensitive (SS) rats with a null mutation for the Nox4 gene (SSNox4-/-) and mice with knockout of the nonselective calcium channel TRPC6 or double knockout of TRPC5 and TRPC6. (siu.edu.ar)
  • H2O2 stimulated TRPC-dependent calcium influx in podocytes from wild-type mice, but this influx was blunted in podocytes from Trpc6-knockout mice and, in a similar manner, in podocytes from Trpc5/6 double-knockout mice. (siu.edu.ar)
  • Finally, electron microscopy revealed that podocytes of glomeruli isolated from Trpc6-knockout or Trpc5/6 double-knockout mice were protected from damage induced by H2O2 to the same extent.Conclusions These data reveal a novel signaling mechanism involving NOX4 and TRPC6 in podocytes that could be pharmacologically targeted to abate the development of DKD. (siu.edu.ar)
  • Conditional Met KO mice were generated using Cre‑loxP methodology and characterization of these mice indicated that the HGF‑Met signaling pathway is essential in regeneration, protection, and homeostasis in various tissue types and cells. (spandidos-publications.com)
  • Early glomerular filtration defect and severe renal disease in podocin-deftcient mice[J]. Mol Cell Biol, 2004, 24(2):550-560. (zjujournals.com)
  • Viable CT-26 cells were implanted into the spleen of these mice. (nartsignaling.com)
  • Nephropathy and Elevated BP in Mice with Podocyte-Specific NADPH Oxidase 5 Expression. (ohri.ca)
  • Podocyte-specific overexpression of human angiotensin-converting enzyme 2 attenuates diabetic nephropathy in mice. (ohri.ca)
  • Podocyte Injury and Albuminuria in Mice with Podocyte-Specific Overexpression of the Ste20-Like Kinase, SLK. (ohri.ca)
  • To mimic activation of Gα12 pathways, constitutively active Gα12 (QL) was conditionally expressed in podocytes using Nphs2-Cre and LacZ/floxed QLα12 transgenic mice. (medscape.com)
  • Osr1 -expressing cell clusters spatially regulate Six2 expression, which in turn induces the cap mesenchymal population. (j-organoid.org)
  • These results suggest that Ang II induces the relocalization and suppression of podocyte p130Cas by the suppression of AMPK via Ang II type 1 receptor, which would contribute to Ang II-induced podocyte injury. (koreamed.org)
  • n\nConclusion: These data suggest that the Pringle maneuver induces hepatic metastasis by stimulating the overexpression of tumor vasculature. (nartsignaling.com)
  • We performed whole animal studies and used biosensor measurements, electron microscopy, electrophysiology, and live calcium imaging experiments to evaluate the contribution of this pathway to the physiology of the podocytes in freshly isolated glomeruli.Results Upon induction of type 1 diabetes with streptozotocin, SSNox4-/- rats exhibited significantly lower basal intracellular Ca2+ levels in podocytes and less DKD-associated damage than SS rats did. (siu.edu.ar)
  • This region, harboring the calpactin light chain binding domain essential for intracellular trafficking of BTV in BSR cells, is lacking in NS3a. (adenosine-receptor.com)
  • Part of cellular calcium is sequestered in intracellular organelles, including endoplasmic reticulum, sarcoplasmic reticulum (SR) in muscle cells and in mitochondria. (abdominalkey.com)
  • When stimulated by agonists of the histamine H1 receptor (H1R), TRPC6 activity decays to the baseline despite the continuous presence of the agonist. (mdpi.com)
  • 2] KHOSHNOODI J, TRYGGVASON K. Unraveling the molecular make-up of the glomemlar podocyte slit diaphragm[J]. Exp Nephrol, 2001, 9(6):355-359. (zjujournals.com)
  • These two events activate ezrin, enabling it to couple transmembrane proteins to the actin cytoskeleton, which could represent a mechanism by which podocyte foot processes form to enable renal filtration. (wikipedia.org)
  • The miR-217 inhibitor and mimics were transfected into human podocyte cells to investigate the pathological mechanism of miR-217 in this disease. (hindawi.com)
  • Unlike classical neurotransmitters, eCBs are not stored in vesicles, and the mechanism of their release from cells is not yet clear. (degruyter.com)
  • Even when released, they remain largely membrane associated due to their hydrophobic nature and can be taken up by cells via a high-affinity uptake mechanism [ 23 ], which is followed by their enzymatic degradation. (degruyter.com)
  • Taken together, the evidence suggests that APOL1-G1 and -G2 risk variants impaired reverse cholesterol transport through decreased expression of cholesterol efflux transporters suggesting a possible mechanism to promote macrophage foam cell formation, driving inflammation in the glomerulus and renal interstitium. (omicsdi.org)
  • Obviously, the main mechanism of egress of non-enveloped viruses is cell lysis, therewith most likely killing the infected mammalian cell. (adenosine-receptor.com)
  • Taken together, these studies suggest that Gα12 activation promotes glomerular injury without podocyte depletion through a novel mechanism regulating collagen (α)IV expression, and supports the notion that glomerular damage may accrue through persistent GPCR activation in podocytes. (medscape.com)
  • CLIC5A is expressed chiefly in the renal glomerulus, specifically in podocytes. (wikipedia.org)
  • A filtrate derived from plasma in the glomerulus must pass through the a basement membrane of the glomerular capillaries and through slits in the processes of the podocytes- the cells that compose the inner layer of the Bowman's capsule. (flashcardmachine.com)
  • Podocytes work with the endothelium of the capillaries to form a thin filter to separate urine from blood passing through the glomerulus. (innerbody.com)
  • They further link to the activation of protein kinase C- (PKC-) induced generation of reactive oxygen species (ROS) [ 6 , 7 ], which further mediates the activation of downstream transcription factor nuclear factor kappa-light-chain enhancer of activated B cells (NF- κ B). Thus, the main treatments of DN refer to modulate glycemic and blood pressure through insulin and RAS inhibitors. (hindawi.com)
  • They consist of a cell body and major and foot processes. (encyclopedia.pub)
  • Between neighboring foot processes, a cell-cell junction is present, forming slit diaphragms that form a size-selective barrier for the excretion of proteins that is critical for proper kidney filtration [ 1 ] . (encyclopedia.pub)
  • This partly results from the fact that glycolysis is the source of adenosine triphosphate (ATP) production in foot processes of podocytes [ 7 ] and that hyperglycemic conditions decrease the dependence of podocytes on oxidative phosphorylation and increase the dependence of podocytes on glycolysis [ 8 ] . (encyclopedia.pub)
  • did not observe CPE of BSR cells infected with BTVM14, which is similar to our mutAUG1 virus, and these authors have suggested a blockade in BTV replication late in infection, since all other viral processes were accomplished like protein translation, genome replication and assembly of viral cores. (adenosine-receptor.com)
  • Promoter region motif analysis indicated that ETV5 has more binding motifs and genes than USF1 itself for USF1‑regulated DEGs, indicating that USF1 may indirectly modulate gene expression by regulating ETV5 expression in Huh7 cells. (spandidos-publications.com)
  • Flow cytometric analysis of human podoplanin expression on human teratocarcinoma cell line. (bdbiosciences.com)
  • The fluorescence histogram showing podoplanin expression (or Ig Isotype control staining), was derived from gated events with the forward and side light-scatter characteristics of viable cells. (bdbiosciences.com)
  • Western-blot analysis was performed to detect the expression of Bcl-2, Bax and Caspase-3 in podocytes. (ijsciences.com)
  • A reduction of 75% in the expression of TK mRNA and protein was observed in HSV-1-infected cells that expressed these functional EGS RNAs. (azd1152.com)
  • We investigated the activity of the EGS in inducing RNase P to cleave the target mRNA and its efficacy in inhibiting HCMV gene expression and growth in cultured cells. (azd1152.com)
  • In MCD mouse model and human kidney tissues, the expressions of podocyte slit membrane protein-nephrin and podocin, skeleton protein-synaptopodin are decreased, and the expression of synaptopodin is correlated with the response to hormone therapy. (zjujournals.com)
  • The pBabe-Id-1 expression retroviral vector and retroviral vectors containing an Id-1-specific small interfering RNA oligonucleotides (si-Id-1) were transfected into 2 bladder cancer cell lines respectively. (chksignal.com)
  • In human monocyte THP-1 cells, transient overexpression of ApoL1 (G0, G1, G2) by transfection resulted in a 5- to 11-fold increase in CD14 and CD68 gene expression, similar to that seen with phorbol-12-myristate acetate treatment. (omicsdi.org)
  • Virus release from BSR cells is solely dependent on NS3 expression, whereas virus release from KC cells is strongly reduced by either lack of NS3, NS3a or both proteins. (adenosine-receptor.com)
  • Expression of SHP-1 induced by hyperglycemia prevents insulin actions in podocytes. (ohri.ca)
  • Angiotensin II (Ang II) directly constricts vascular smooth muscle cells, stimulates aldosterone production, activates the sympathetic nervous system, and increases sodium reabsorption, therefore, contributes to the development of hypertension ( 1 2 ). (koreamed.org)
  • Normally, activation of the CB 1 receptor regulates renal vascular hemodynamics and stimulates the transport of ions and proteins in different nephron compartments. (degruyter.com)
  • APOL1 is a minor protein component of HDL, as well as being expressed in podocytes and vascular cells. (omicsdi.org)
  • While the organoids grow and self-assemble from stem cells, their in vitro development deprives them of the vascular perfusion otherwise available in vivo . (corning.com)
  • 4. These findings suggest that endothelial-cell-stimulating angiogenic activity in muscle is either inversely or not related to the local capillary density, which may be at or near a maximum in physiologically contracting, predominantly oxidative muscles. (portlandpress.com)
  • The damage to these cells has been attributed in part to elevated intrarenal oxidative stress. (siu.edu.ar)
  • The activity of AMPK has been closely related to oxidative stress in podocyte injury models, such as, diabetic and adriamycin-induced oxidative podocyte injury ( 12 13 14 ). (koreamed.org)
  • In various mouse and rat models of obesity and type 1 and 2 diabetes mellitus, eCBs generated in various renal cells activate CB 1 receptors and contribute to the development of oxidative stress, inflammation, and renal fibrosis. (degruyter.com)
  • We have now determined that in cultured podocytes, high glucose led to increases in activation of EGFR signaling but decreases in autophagy activity as indicated by decreased beclin-1 and inhibition of LC3B autophagosome formation as well as increased rubicon (an autophagy inhibitor) and SQSTM1 (autophagy substrate). (diabetesjournals.org)
  • Vitamin D3 was reported to provide renal protection in DKD through the Vitamin D receptor (VDR), however there was limited data on whether it has a protective effect on podocytes of DKD induced by high glucose. (ijsciences.com)
  • Methods: The experimental subjects were conditionally immortalized mouse podocytes (MPC-5), which were divided into 4 groups: NG (normal glucose) group, HM (Hypertonic) group, HG (high glucose) group, VD3 (HG +1,25-dihydroxyvitamin D3 (1,25-D3)) group. (ijsciences.com)
  • High glucose concentration affects the oxidant‐antioxidant balance in cultured mouse podocytes[J]. Journal of Cellular Biochemistry, 2011, 112(6):1661-1672. (ijsciences.com)
  • Ji Z Z, Xu Y C. Melatonin protects podocytes from angiotensin II-induced injury in an in vitro diabetic nephropathy model[J]. Molecular Medicine Reports, 2016, 14(1):920. (ijsciences.com)
  • Podocyte injury in diabetic nephropathy: implications of angiotensin II - dependent activation of TRPC channels[J]. Scientific Reports, 2015, 5:17637. (ijsciences.com)
  • Gα12/13 are expressed in podocytes and couple to angiotensin II, thrombin, endothelin and LPA receptors, which are important in renal injury. (medscape.com)
  • This application of force recruited Rho and filamentous actin (F-actin) to bead loci and subsequently stimulated phospholipase D (PLD), a downstream effector of Rho. (elsevierpure.com)
  • We have previously found that Ang II suppressed podocyte AMP-activated protein kinase (AMPK) via Ang II type 1 receptor and MAPK signaling pathway. (koreamed.org)
  • In the present study, we investigated the roles of AMPK on the changes of p130Cas of podocyte by Ang II. (koreamed.org)
  • We cultured mouse podocytes and treated them with various concentrations of Ang II and AMPK-modulating agents and analyzed the changes of p130Cas by confocal imaging and western blotting. (koreamed.org)
  • AMPK is expressed in essentially all eukaryotic cells from yeast to plants and animals. (koreamed.org)
  • In podocytes, the increased activity of AMPK by adiponectin treatment in cultured podocytes reduced podocyte permeability to albumin and podocyte dysfunction ( 12 ). (koreamed.org)
  • Recently, we have found that Ang II suppressed podocyte AMPK via AT1R and MAPK signaling pathway ( 15 ). (koreamed.org)
  • Chihara (2018) associated Podoplanin with a co-inhibitory receptor in T-cell activation along with PD-1, TIM-3, LAG-3 and TIGIT. (absoluteantibody.com)
  • The receptor is normally avoided by This technique from time for the cell surface area, where in fact the receptor could remove even more LDL particles. (researchensemble.com)
  • Targeting activated Gα subunits to specific cells in vivo permits identification of downstream effector pathways independent of receptor activation, and thus permits insight into disease mechanisms otherwise impossible to study in vivo . (medscape.com)
  • Podocytes are highly specialized cells that are dedicated to plasma filtration. (encyclopedia.pub)
  • The dysregulated podocyte phenotype: a novel concept in the pathogenesis of collapsing idiopathic focal segmental glomerulosclerosis and HIV-associated nephropathy[J]. J Am Soc Nephrol, 1999, 10(1):51-61. (zjujournals.com)
  • We hypothesized that podocytes are susceptible to filtered plasma components, including hormones and growth factors that stimulate signaling pathways leading to glomerulosclerosis. (medscape.com)
  • In recent years more studies show that podocyte injury is the key link in the pathogenesis of MCD. (zjujournals.com)
  • Urinary Podocyte Microparticles Identify Prealbuminuric Diabetic Glomerular Injury. (ohri.ca)
  • Podocyte injury associated with mutant alpha-actinin-4. (ohri.ca)
  • The use of this antibody in immunostaining of somatic tissues, including mouse lung lymphatic vessels, kidney glomeruli, pulmonary alveoli, pulmonary pleura and salivary gland myoepithelial cells, has been successfully performed on both paraffin-embedded sections and frozen sections (Kaji et al, 2012). (absoluteantibody.com)
  • Hyperglycemic conditions (HG), at early stages of diabetic nephropathy (DN), cause a decrease in podocyte numbers and an aberration of their function as key cells for glomerular plasma filtration. (encyclopedia.pub)
  • Mechanistically, cGAS upregulated the IFN-I signaling pathway by directly interacting with STING and TBK1, contributing to CD4+ T cell activation. (bvsalud.org)
  • Studies of cultured podocytes demonstrate that they respond to stretch by altering the structure of the actin cytoskeleton, but the mechanisms by which physical force triggers this architectural change and the signaling pathways that lead to generation of second messengers are not defined. (elsevierpure.com)
  • Using MDCK cells that stably express regulators of G protein-signaling (RGS) proteins [RGS4 attenuates Gα i and Gα q , and the p115RhoGEF-RGS domain (p115-RGS) attenuates Gα 12/13 ] to define the signaling pathway, we found that mechanical force induced Gα 12/13 -Rho activation and increased F-actin to stimulate PLD activity. (elsevierpure.com)
  • Klotho is also a coreceptor for fibroblast growth factor receptors (FGFRs), the signaling of which, together with a proper rate of glycolysis in podocytes, is needed for a proper function of the glomerular filtration barrier. (encyclopedia.pub)
  • For example, ribozymes have been shown to cleave viral mRNA sequences and inhibit viral replication in human cells (7C9). (azd1152.com)
  • Generally, replication of all BTV mutants was CASIN custom synthesis similar to wtBTV1/8 in KC and BSR cells for the first 24 hours post infection but after this period, differences were observed in 25837696 released virus as well as in cell-associated fractions. (adenosine-receptor.com)
  • Cytosolic calcium can be measured by loading the tested cells with a fluorescent probe such as indo-1-acetoxymethyl ester and exciting the cells at 350 nM. (abdominalkey.com)
  • In certain types of cells, a Na + /Ca 2+ exchanger, energized by a Na + -gradient, helps drive cytosolic calcium into the extracellular space. (abdominalkey.com)
  • Since the first publication on generating kidney-like cell aggregates from pluripotent stem cells, various modifications have been made to develop more complex and detailed kidney structures. (j-organoid.org)
  • Freedman was the first scientist in the western hemisphere to generate a kidney organoid from pluripotent stem cells. (corning.com)
  • The Matrigel matrix forms a crucial component of the kidney organoid generation process from pluripotent stem cells. (corning.com)
  • First, endothelin is produced by and acts upon virtually every cell type in the kidney. (medtelligence.net)
  • Endothelin preferentially stimulates efferent arteriolar constriction, thereby increasing interglomerular pressure. (medtelligence.net)
  • Macrophage stimulating protein (MSP) was discovered as a serum protein that promoted mouse macrophage motility ( 13 ), and was later purified to homogeneity from human plasma ( 14 ). (spandidos-publications.com)
  • By seeding the stem cell-derived kidney cells on this matrix-coated chip, the cells can be grown into controlled shapes and arrangements. (corning.com)
  • Moreover, kidney cells were found to be the main source of the soluble form of Klotho, which is considered to be a hormone that exerts beneficial effects on target tissues [ 17 ] . (encyclopedia.pub)
  • The tubule cells that line the proximal convoluted tubule reabsorb much of the water and nutrients initially filtered into the urine. (innerbody.com)
  • Synaptopodin: an actin-associated protein in telencephalic dendrites and renal podocytes[J]. J Cell Biol, 1997, 139(1):193-204. (zjujournals.com)
  • Distribution of podocyte protein(44 KD)in different types of glomerular diseases[J]. Virchows Arch, 1997, 431(6):425-430. (zjujournals.com)
  • p130Cas was initially discovered as a 130 kDa protein which is highly tyrosine phosphorylated in cells expressing p47 v-Crk (C10 regulator of kinase) and p60 v-Src (for sarcoma) oncoproteins ( 16 17 18 ). (koreamed.org)
  • 10%. In the remaining 10% of children with lecular weight protein found on the surface nephrotic syndrome, it is largely mediated of all nucleated cells which synthesize it. (who.int)
  • The existence of specific receptors for plant-derived molecules in mammalian cells initiated a search for specific endogenous ligands. (degruyter.com)
  • Since virus release from insect cells occurs by budding, these results suggest that both proteins are FCCP site involved in budding, and thus also suggests a specific role for NS3a. (adenosine-receptor.com)
  • Further research is needed to unravel the specific role of each of these non-structural proteins in virus release from insect cells. (adenosine-receptor.com)
  • Huh7 cells that overexpress USF1 were used with whole transcriptome profiling through RNA sequencing and chromatin immunoprecipitation (ChIP) sequencing methods to investigate the downstream targets of USF1. (spandidos-publications.com)
  • With impaired reverse cholesterol transport, lipid accumulation occurs and macrophages morphologically transform into foam cells, releasing inflammatory factors. (omicsdi.org)
  • HGF is produced and secreted by adjacent stromal and mesenchymal cells, it contributes to the development of epithelial organs in a paracrine fashion, exerts regenerative effects on epithelia in the liver, kidney, lung, and other tissues, and promotes the regression of fibrosis in numerous organs ( 7 , 8 ). (spandidos-publications.com)
  • Within the cell, CLIC5A is localized to the plasma membrane and the cytosol, and associates and is regulated by the actin cytoskeleton. (wikipedia.org)
  • BTV release from BSR cells is mainly dependent on CPE through the permeabilization of the membrane likely induced by NS3, whereas BTV release from KC cells is dependent on budding. (adenosine-receptor.com)
  • It stimulates insertion of aquaporins (water channels) into the plasma membrane of the collecting duct. (flashcardmachine.com)
  • For arthropod borne orbiviruses, cell lysis has not been observed in insect cells in contrast to cell lysis of mammalian cells. (adenosine-receptor.com)
  • Obviously, NS3 plays an important role in BTV release from mammalian cells, whereas both NS3 and NS3a are important for release from KC cells. (adenosine-receptor.com)
  • Nephrotic syndrome may occur in persons with sickle cell disease and evolve to renal failure. (medscape.com)
  • NF-κB pathways are associated with immunity, inflammation and cell survival. (springer.com)
  • NCCIT cells (ATCC CRL-2073) were stained with either PE Mouse IgG1, κ Isotype Control (Cat. (bdbiosciences.com)
  • This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin. (absoluteantibody.com)
  • This antibody is specific for amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin (PDPN). (absoluteantibody.com)
  • introduced a new concept of CKD-associated secretory phenotype (CASP), which indicates that senescent renal cells could secrete SASP components of various cytokines such as IL-1, IL-6, and TNF- α [ 19 , 20 ]. (hindawi.com)
  • The human kidney contains approximately one million nephrons, each composed of at least 20 different cell types. (j-organoid.org)
  • Pretreatment with vitamin D3 attenuated these abnormalities in podocytes in HG group. (ijsciences.com)
  • Pretreatment of the cells with chemical inhibitors of several kinases showed that calmodulin-dependent kinase is also involved in stretch-induced PLD activation by a separate pathway. (elsevierpure.com)
  • Conversely, mutation of Id3, known to cause antibody-mediated autoimmunity, greatly enhances T-FH-cell generation. (nartsignaling.com)
  • In the present study, we found that in renal epithelial cells [podocytes and Madin-Darby canine kidney (MDCK) cells], application of mechanical force to the cell surface through fibronectin-coated ferric beads and exposure of the cells to magnetic force lead to Rho translocation and actin cytoskeleton reorganization. (elsevierpure.com)
  • Taken together, our data demonstrate that in cultured podocytes and MDCK cells, mechanical force leads to actin cytoskeleton reorganization and PLD activation. (elsevierpure.com)