• neurons
  • Moreover, primary cultured dopaminergic neurons from mesencephalon were more sensitive to rotenone-induced cell death than nondopaminergic neurons in the same culture. (jneurosci.org)
  • When a short hairpin for Nurr1 was expressed in microglia and astrocytes, these cells produced inflammatory mediators, such as TNFa, NO synthase and IL-1β, supporting the conclusion that reduced Nurr1 promotes inflammation and leads to cell death of dopaminergic neurons. (wikipedia.org)
  • Additionally, omigapil can prevent NMDA and kainate receptor excitotoxicity in rat cortical neurons as well as toxicity from cytosine arabinoside (ara C) in cerebellar granule cells. (wikipedia.org)
  • Here, we showed that rotenone-induced toxicity in human neuroblastoma cell lines and mouse primary cortical neurons was ameliorated by PTP1B inhibition. (molcells.org)
  • Moreover, we found that MG132-induced toxicity involving proteasome inhibition was also ameliorated by PTP1B inhibition in a human neuroblastoma cell line and mouse primary cortical neurons. (molcells.org)
  • The protein kinase MST1 (mammalian Ste20-like kinase 1) plays a major role in oxidative stress-induced cell death in primary mammalian neurons. (jneurosci.org)
  • Oxidative stress induces the c-Abl-dependent tyrosine phosphorylation of MST1 and increases the interaction between MST1 and FOXO3 (Forkhead box O3), thereby activating the MST1-FOXO signaling pathway, leading to cell death in both primary culture neurons and rat hippocampal neurons. (jneurosci.org)
  • Inhibition of c-Abl by using c-Abl RNAi or STI571 attenuates oxidative stress-induced MST1 activation as well as cell death both in primary cultured neurons and in rat hippocampal neurons. (jneurosci.org)
  • Taken together, we suggest that BV treatment can be useful for protection of neurons against oxidative stress or neurotoxin-induced cell death. (blogspot.com)
  • found that over-expression of human wide-type LRRK2 or the G2019S mutant in flies triggers marked degeneration in their photoreceptor cells and DA neurons in general. (jneurosci.org)
  • In vitro studies with dopaminergic neuron cell cultures demonstrated increased expression of tyrosine hydroxylase and associated transcription factors, increased neurite outgrowth, regeneration of neurons after chronic rotenone administration, and reduced expression of inflammatory cytokines. (wikipedia.org)
  • In studies of primary mesencephalic dopaminergic neuron cell cultures, the substance increased the number of differentiated dopaminergic neurons and produced higher levels of transcription factors associated with dopaminergic differentiation. (wikipedia.org)
  • Rodent studies in vivo demonstrated elevated hippocampal dopamine levels, improved spatial learning performance in a radial maze test, and increased dendrite outgrowth in the dentate gyrus of the hippocampus, as well as restoration of the number of tyrosine hydroxylase expressing neurons in the left striatum after an injection of MPP+ had reduced the number of such cells by 50% in an animal model of Parkinsonism. (wikipedia.org)
  • The reason for this cell death is poorly understood, but involves the build-up of proteins into Lewy bodies in the neurons. (wikipedia.org)
  • induce
  • However, recent studies have demonstrated that rotenone has the potential to induce several adverse effects such as a neurodegenerative disease. (sigmaaldrich.com)
  • This study suggests a new mechanism of higher ROS production in ovarian cancer cells and provides strong evidence that endogenous ROS play an important role for cancer cells to induce angiogenesis and tumor growth. (aacrjournals.org)
  • apoptotic
  • Our results showed rotenoisin B inhibited hepatic cancer cells' proliferation in a dose dependent manner and increased in apoptotic cells. (sigmaaldrich.com)
  • The compound displayed cell-rescuing effects in various models of apoptotic neuronal death, as well as in rodent and non-rodent animal models of neurodegeneration. (wikipedia.org)
  • toxins
  • and it can protect still other cells from known toxins (Wink et al. (bio.net)
  • The study does not directly suggest rotenone exposure is responsible for PD in humans, but is consistent with the belief that chronic exposure to environmental toxins increases the likelihood of the disease. (wikipedia.org)
  • exposure
  • Fluorescence recovery after photobleaching assays demonstrated a lower level of functional connectivity of the mitochondrial matrix, which further worsened after rotenone exposure. (nih.gov)
  • vascular endoth
  • Accordingly, we treated human coronary artery endothelial cells (HCAECs) with either 50 ng/ml vascular endothelial growth factor (VEGF), 2 uM Rotenone (Rot) or VEGF and Rot, respectively, in incubators supplemented with either 20.0% (normoxic) or 2.5% (hypoxic) oxygen for 48 hr. (ahajournals.org)
  • To analyze the functional relevance of ROS production, we showed that ROS regulated hypoxia-inducible factor 1 (HIF-1) and vascular endothelial growth factor (VEGF) expression in ovarian cancer cells. (aacrjournals.org)
  • derris
  • Rotenone, isolated from roots of derris plant, has been shown to possess various biological activities, which lead to attempting to develop a potent drug against several diseases. (sigmaaldrich.com)
  • Rotenone is produced by extraction from the roots and stems of several tropical and subtropical plant species, especially those belonging to the genera Lonchocarpus and Derris. (wikipedia.org)
  • transmembrane
  • The endoplasmic reticulum (ER) is a multifunctional continuous membrane system essential for the synthesis, folding and processing of secretory and transmembrane proteins in eukaryotic cells. (molcells.org)
  • Cyanide
  • Although cyanide stimulates the peripheral respiratory chemoreceptors, increasing the inspired oxygen fraction is not helpful, since there is already an adequate amount of oxygen which the poisoned cells cannot use. (wikipedia.org)
  • Examples of respiratory blockers are rotenone and cyanide. (wikipedia.org)
  • VEGF
  • NOX4 knockdown in ovarian cancer cells decreased the levels of VEGF and HIF-1α and tumor angiogenesis. (aacrjournals.org)
  • survival
  • Oxidative stress influences cell survival and homeostasis, but the mechanisms underlying the biological effects of oxidative stress remain to be elucidated. (jneurosci.org)
  • cellular
  • Typically, rotenone-containing plants in the Fabaceae family of legumes are crushed and introduced into a body of water, and as rotenone interferes with cellular respiration, the affected fish rise to the surface in an attempt to gulp air, where they are more easily caught. (wikipedia.org)
  • roots
  • Rotenone occurs in the roots, stems, and leaves of tropical plants in the pea family ( Fabaceae ). (nps.gov)
  • formulations
  • Biologists in Yellowstone National Park have used rotenone in formulations approved by the Environmental Protection Agency in High Lake and East Fork Specimen Creek (2006-2009), Goose Lake (2011), Elk Creek (2012-2014), Grayling Creek (2013-2014), and Soda Butte Creek (2015- 2016) to remove non-native fish species. (nps.gov)
  • occurs
  • Importantly, coexpression of human parkin in LRRK2 G2019S-expressing flies provides significant protection against DA neurodegeneration that occurs with age or in response to rotenone. (jneurosci.org)
  • The target site and mechanism of toxic action through which narcosis affects organisms are still unclear, but there are hypotheses that support that it occurs through alterations in the cell membranes at specific sites of the membranes, such as the lipid layers or the proteins bound to the membranes. (wikipedia.org)
  • death
  • Reducing the expression of the proapoptotic protein Bcl-2-associated death protein (BAD) by small interfering RNA rendered SH-SY5Y cells resistant to rotenone, implicating BAD in rotenone-induced cell death. (jneurosci.org)
  • Essentially, rotenone starves the cells, causing death. (nps.gov)
  • In mammalian systems, mammalian Ste20-like kinase (MST) is ubiquitously expressed and is best known for its function in promoting cell death. (jneurosci.org)
  • The mechanisms leading to MST activation and cell death vary depending on stimulus specificity and cell type. (jneurosci.org)
  • In fact, there has been shown to be a correlation between mitochondrial activities and programmed cell death (PCD) during somatic embryo development. (wikipedia.org)
  • The motor symptoms of the disease result from the death of cells in the substantia nigra, a region of the midbrain. (wikipedia.org)
  • MPP+ interferes with complex I of the electron transport chain, a component of mitochondrial metabolism, which leads to cell death and causes the buildup of free radicals, toxic molecules that contribute further to cell destruction. (wikipedia.org)
  • MPP+ has quite selective abilities to cause neuronal death in dopaminergic cells, it is presumed through a high-affinity uptake process in nerve terminals normally used to reuptake dopamine after it has been released into the synaptic cleft. (wikipedia.org)
  • epithelial cells
  • The human ovarian cancer cells OVCAR-3 and A2780 and immortalized ovarian surface epithelial cells IOSE 397 and IOSE 386 were maintained in RPMI 1640 (Invitrogen) supplemented with 10% fetal bovine serum (FBS), 2 mmol/L l -glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin and cultured at 37°C in a 5% CO 2 incubator. (aacrjournals.org)
  • organic
  • Rotenone is quickly broken down in the environment by sunlight and readily binds to sediments or organic matter in the water. (nps.gov)
  • Rotenone has been used as an organic pesticide dust for gardens. (wikipedia.org)
  • periods
  • To reduce potential impacts on non-target organisms specialists use a minimum dosage of rotenone, for short periods of time. (nps.gov)
  • mechanisms
  • Glutathione S -transferase Omega (GSTO) is involved in cell defense mechanisms, but little is known about the role of GSTO in the progression of Parkinson disease. (pubmedcentralcanada.ca)
  • some superfamily members are known to be involved in cell defense mechanisms ( 3 , 4 ). (pubmedcentralcanada.ca)
  • ERK1
  • bFGF activated both extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphatidylinositol-3 kinase (PI3-kinase) pathways in SH-SY5Y cells. (jneurosci.org)
  • treatment
  • We show that betaine treatment leads to an upregulation of mitochondrial respiration and cytochrome c oxidase activity in H2.35 cells, the proposed rate limiting enzyme of ETC in vivo. (osti.gov)
  • human
  • This information may be useful to understand the new mechanism of cancer cells in inducing tumorigenesis and to develop new therapeutic strategy by targeting ROS signaling in human cancer in the future. (aacrjournals.org)
  • Human deaths from rotenone poisoning are rare because its irritating action causes vomiting. (wikipedia.org)