• A molecular explanation for this may be that several of the engulfment genes expressed by macrophages, including the ABC1 transporter (believed to be part of the phagocytic machinery conserved from Caenorhabditis elegans to mouse), are not upregulated by these 'stand-in' phagocytes. (bris.ac.uk)
  • Tumorigenesis is a multi-step process in which a normal cell acquires changes in a number of critical cancer causing genes. (umassmed.edu)
  • Interestingly, our results also show that the AHR antagonist resveratrol, markedly attenuated BNF-induced liver mitochondrial defects in wild-type mice, confirming the role of AHR and AHR-regulated CYP1 genes in eliciting mitochondrial dysfunction. (uaeu.ac.ae)
  • Gene expression studies performed on PPARα null mice have shed light into a variety of genes regulated by PPARα. (wur.nl)
  • To understand regulation of gene expression and to delineate the function of mammalian genes, we routinely create transgenic & gene knockout mice by performing DNA microinjection into mouse eggs, and genetic manipulation of mouse embryonic stem cells. (ccmb.res.in)
  • A striking number of cardiovascular defects of humans are modeled in mice with mutation(s) in one or more genes encoding TGFβ ligands. (sc.edu)
  • This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
  • More importantly, biotechnologists will for the first time be able to manipulate the genes of cells from farm animals directly before growing them into embryos. (newscientist.com)
  • But scientists have not managed to isolate such cells from farm animals, and must rely instead on injecting genes randomly into early embryos. (newscientist.com)
  • Subsequent experiments in mice using the new enzymes, packaged with partner genes in an AAV, confirmed efficient knock-in and knockout effects. (genomeweb.com)
  • Behavioral characterization of genetically modified mice for schizophrenia-associated susceptibility genes. (iit.it)
  • In the early 1980s, researchers began genetically manipulating mice by inserting genes from other species (including humans) during the earliest stages of embryonic cell division. (mentalfloss.com)
  • Scientists also began turning off specific genes during early development, creating 'knockout mice. (mentalfloss.com)
  • Since identification of EZH2 in the research of protooncogene product Vav [ 3 ], studies have shown that EZH2 is highly expressed in tumorigenesis, which regulates the expression of tumor suppressor genes, such as in breast cancer, prostate cancer, and lung cancer [ 4 - 6 ]. (hindawi.com)
  • To determine the potential signaling pathway of lncRNA Gm2044 regulating estradiol synthesis, transcriptome sequencing was performed for ovaries of adult lncRNA Gm2044 knockout mice, which identified 565 significant up-regulated genes and 303 significant down-regulated genes, which were then analyzed with Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) and validated by molecular experiments. (bvsalud.org)
  • T6081The Stable Bcl-xL Mouse Embryonic Fibroblast Cell Line was derived from primary embryonic cells from an 11-d-pregnant Bcl-x. (topsan.org)
  • A paper published electronically this week by Nature Genetics offers important new insights into the development and differentiation of rod and cone photoreceptors, the light-sensitive cells in the eye's retina that initiate vision and are essential for clear sight. (sciencedaily.com)
  • In addition, because the precursor, or stem, cells that give rise to rods and cones experience delays before expressing a specific cell type, there must be some additional cues that signal rod differentiation. (sciencedaily.com)
  • Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
  • In this issue of Cancer Discovery , Dominguez and colleagues report that TET2 function is critical for germinal center exit and plasma cell differentiation, and its deficiency can lead to B-cell lymphoma phenotypes. (aacrjournals.org)
  • Furthermore, the decreased expression of the EZH2 gene is crucial for stem cell differentiation into specific cell lineages involved in myogenesis, adipogenesis, osteogenesis, neurogenesis, and haematopoiesis [ 9 ]. (hindawi.com)
  • At the molecular level, OPN promotes the expression and activation of TGF-β1, stimulates the differentiation of myofibroblasts from fibroblasts, and increases the production of fibrous matrix proteins in lungs and cultured lung cells exposed to MWCNTs. (cdc.gov)
  • Firstly, we present a robust 2-week protocol for the differentiation of human pluripotent stem cells (PSCs) into forebrain neural progenitor cells. (lu.se)
  • If you were to pluck two cells from a chimeric mouse's body, you could get two different genomes - complete sets of chromosomes and genetic information. (livescience.com)
  • Carstea AC, Pirity MK, Dinnyes A. Germline competence of mouse ES and iPS cell lines: Chimera technologies and genetic background. (wjgnet.com)
  • The development and progression of tumors is taught on a molecular genetic and cell biological basis. (tum.de)
  • Sir Martin performed pioneering research into stem cells, and used them to create mice with a specific genetic disorder. (lirneasia.net)
  • In addition to these basic issues concerning leukemia pathogenesis, we are devising new diagnostic procedures for detecting and monitoring leukemia patients based on molecular genetic abnormalities in the malignant cells. (stanford.edu)
  • Next, the researchers take cells containing donor genetic material. (newscientist.com)
  • Finally, Little made a crucial decision that would change medical and genetic research forever: He compiled a catalog of the inbred strains he'd created and used for his own research, and he offered to sell it to other institutions. (mentalfloss.com)
  • More so, TEs are a rich source of genetic variation, which makes them an intriguing research avenue to investigate humanspecific traits, including their impact on human brain evolution and their relevance in disease. (lu.se)
  • He also developed conditional knockout mice based on Cre-Lox recombination. (wikipedia.org)
  • 1)Study mechanisms of retinal iron regulation by performing histology and immunofluorescence on retinal sections from conditional knockout mice. (upenn.edu)
  • To this end we used a HILPDA tissue-specific knockout mice model in macrophages and hepatocytes generated by using LysM-Cre and Alb-Cre transgenic mice, respectively. (wur.nl)
  • A modern mouse facility houses transgenic mice and other experimental mouse strains needed for creation of transgenic and gene knock-out mice. (ccmb.res.in)
  • The result was 'transgenic mice. (mentalfloss.com)
  • 5' noncoding region] and significantly lower neurovirulence supplementary immunization activity (SIA) to sample collection of fecally shed vaccine virus in transgenic mice) ( 3 - 5 ). (cdc.gov)
  • Furthermore, aberrant oncogenic activation, DNA damage or oxidative stress activates senescence, providing a failsafe mechanism that prevents the proliferation of cells at risk of neoplastic transformation. (umassmed.edu)
  • A) Lymphomas found in Smurf2-deficient mice have the characteristics of diffuse large B-cell lymphoma (DLBCL) and enhanced cell proliferation. (umassmed.edu)
  • It also enhances B cell survival, proliferation, and antibody production. (thermofisher.com)
  • In cardiac conditional Ezh2-knockout mice, cardiomyocyte proliferation was repressed, and the apoptosis process was induced [ 10 ]. (hindawi.com)
  • instead, we found that PDGF-DD stimulated bulk tumor cell proliferation by induction of paracrine mitogenic signaling between heterogeneous malignant cell clones, some of which expressed PDGFRβ. (lu.se)
  • We have shown that LTD4 via its receptor CysLT1 induces expression of proteins associated with colorectal cancer and promotes proliferation, survival and migration in intestinal epithelial cells. (lu.se)
  • T6080The Stable Bcl-x Knockout Mouse Embryonic Fibroblast Cell Line was generated from embryos of a pregnant mouse heterozygous for. (topsan.org)
  • To create the chimeric monkeys, researchers essentially glued together cells from individual rhesus monkey embryos and then implanting these mixed embryos into mama monkeys. (livescience.com)
  • The key was mixing cells from very early-stage embryos, or blastocysts, that consisted of just two to four cells - each one of the cells still totipotent, capable of transforming into a whole animal as well as the placenta and other life-sustaining tissues. (livescience.com)
  • Researchers in Jun Wu's lab compare normal mouse embryos (left) to horse-mouse chimeric embryos (right) to identify barriers to interspecies chimerism. (the-scientist.com)
  • The mouse genetics laboratory includes well-furnished infrastructure for culture of embryonic stem cells, cryopreservation of embryos, micro-manipulation of embryos, animal surgery, construction of transgenes & gene knock-out vectors, identification of transgenic & knock-out mice, and analysis of gene expression. (ccmb.res.in)
  • A year ago, we showed that you could do it with cells from embryos," says Wilmut. (newscientist.com)
  • Particularly valuable animals could be cloned from adult cells without the uncertainties of crossing them with other animals or tinkering with embryos. (newscientist.com)
  • Established in response to a need for in-house mouse services, the LUTCF provides expertise in cryopreservation of embryos by IVF or natural matings, sperm cryopreservation, rederivation services, strain expansion by IVF, ES morula/blastocyst injections, pronuclear DNA microinjections, and injection of CRISPR edited DNA. (lu.se)
  • This is currently the most popular method for the generation of targeted knock-out and knock-in models by ES-cell injections into 8-cell morulae and/or blastocysts of mouse embryos. (lu.se)
  • The phenotype of the L1 mutant mouse indicates that it is a potentially valuable model for the human CRASH syndrome. (jneurosci.org)
  • Homozygous CyP-D knock-out mice display regular phenotype (Basso et al. (mingsheng88.org)
  • Conversion of Peripheral Blood NK Cells to a Decidual NK-like Phenotype by a Cocktail of Defined Factors The Journal of Immunology, 190:3939-3948 (2013). (bioind.com)
  • Methods: C57BL/6J (WT) and Opn knockout (KO) mice were exposed to MWCNTs by pharyngeal aspiration to examine the acute and chronic effects of MWCNT exposure. (cdc.gov)
  • Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. (cdc.gov)
  • B6.129X1-MPO (MPO k/o) vs wild-type C57Bl/6 mice (w/t) and induction of mesotheliomas) [8,9]. (cdc.gov)
  • Apoptosis is one of the key tools used by an embryo to regulate cell numbers and sculpt body shape. (bris.ac.uk)
  • they found that cells from the knockout mice failed to undergo apoptosis and lacked nuclear translocation of the mitochondrial flavoprotein apoptosis-inducing factor (AIF) . (the-scientist.com)
  • In the motor, somatosensory, and visual cortex, many pyramidal neurons in layer V exhibited undulating apical dendrites that did not reach layer I. The hippocampus of L1 mutant mice was smaller than normal, with fewer pyramidal and granule cells. (jneurosci.org)
  • L1 has been localized to growth cones and processes of postmitotic developing neurons, where it mediates cell adhesion, neurite outgrowth, and axon bundling. (jneurosci.org)
  • Neurons that arise in the adult nervous system originate from neural stem cells and neural progenitor cells. (elifesciences.org)
  • Neural stem cells can also give rise to neural progenitor cells, which proliferate rapidly during their short lives and then 'differentiate' into neurons or glia. (elifesciences.org)
  • Since neurosphere-forming cells can self-renew and differentiate into neurons and glia, the ability of cells to form neurospheres has generally been taken as evidence that they are stem cells. (elifesciences.org)
  • They show that treating cultured mouse neurons expressing human APP with the acetylcholine receptor agonist carbachol increased the production of α-secretase cleavage products, but no such effect occurred in cells from M1 knockout mice. (alzforum.org)
  • In addition, Davis and coworkers show that treating neurons from M1 knockout mice with carbachol increased Aβ production, probably via stimulation of other non-M1 muscarinic receptor subtypes. (alzforum.org)
  • This research by itself should help scientists in conducting biomedical research more relevant to humans, he said. (livescience.com)
  • In humans, mutations in the L1 cell adhesion molecule are associated with a neurological syndrome termed CRASH, which includes corpus callosum agenesis, mental retardation, adducted thumbs, spasticity, and hydrocephalus. (jneurosci.org)
  • So far, the expression pattern and the function of EZH2 have been broadly studied in humans [ 11 ] and mice [ 12 , 13 ]. (hindawi.com)
  • If you see this knockout in humans, they really don't respond well to any therapy other than stem cell transplant. (medscape.com)
  • Herein, we showed that glibenclamide promoted insulin release and further activated autophagy through the adenosine 5′-monophosphate (AMP) activated protein kinase (AMPK) pathway in MIN-6 cells. (hindawi.com)
  • The fact that mice lacking the protein have no obvious health problems is encouraging, suggesting that Abcc10 inhibitors could be used in human patients without causing side effects that might be expected to result from interfering with the pump's normal functions. (news-medical.net)
  • Conditional deletion of the Bmi-1 polycomb protein depleted pre-GEPCOT and GEPCOT cells, though pre-GEPCOT cells were more dependent upon Bmi-1 for Cdkn2a ( p16 Ink4a ) repression. (elifesciences.org)
  • On the other hand, manifestation of CyP-D, a soluble matrix protein, is definitely associated with mPTP opening and cell death during ischemia/reperfusion in the heart and mind. (mingsheng88.org)
  • CyP-D is definitely a nuclear encoded protein widely indicated in all mammalian cells. (mingsheng88.org)
  • The nuclear enzyme poly(ADP-ribose) polymerase-1 (PARP-1) kills cells by inducing translocation of a mitochondrial suicide protein. (the-scientist.com)
  • The BOB.1 / OBF.1 / OCA-B protein (henceforth designated as BOB.1 / OBF.1) is a B cell-specific co-activator of the Oct1 and Oct2 transcription factors. (nih.gov)
  • Thus, these results demonstrate that BOB.1 / OBF.1 itself is a non-redundant protein in B cells and absolutely required for octamer-dependent transcriptional activity. (nih.gov)
  • A team led by Anand Swaroop, Ph.D., professor of ophthalmology and vision research scientist at the University of Michigan Health System's Kellogg Eye Center, has demonstrated that the retinal protein Nrl is required for rod development and, in fact, acts as a "molecular switch," signaling the cells to develop into rods rather than cones. (sciencedaily.com)
  • To this end, we currently use several (conditional) knock-out mice and cell lines to study gene expression patterns, metabolic profiles, protein interactions, intracellular trafficking, and signaling pathways. (nih.gov)
  • In hepatoma cell lines, fatty acids increase Hilpda expression and protein levels. (wur.nl)
  • With an $80,000 grant from FRAXA Research Foundation from 2005-2006, Dr. David Morris and his team at the University of Washington aimed to understand the variation in distribution and function of FMRP isoforms, sought to identify isoforms of FMRP in mouse brain, and define the expression pattern of these versions of the protein. (fraxa.org)
  • We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)
  • The compact size of the new enzyme variants means that a greater quantity of the gene editing protein can be packaged into vectors such as adeno-associated viruses (AAVs) commonly used to infect living human cells requiring DNA editing. (genomeweb.com)
  • DNAJB6 is an essential protein and knock-out mice die already in uteri due to failure in keratin turnover and placental development. (lu.se)
  • IGSF1 was localized to thyrotrope cells, implicating the protein in pituitary control of the thyroid. (medscape.com)
  • We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)
  • To investigate the mechanisms of retinal iron regulation, the lab uses conditional knockout mouse models, human retinal tissue, and retinal cell tissue culture. (upenn.edu)
  • The research contained in this thesis first sought to improve our current knowledge on the transcriptional regulation by peroxisome proliferator-activated receptors (PPAR)-α activation on human liver in vivo using a novel humanized-liver mouse model. (wur.nl)
  • The role of PPARα in gene regulation in mouse liver is well characterized. (wur.nl)
  • Our results support the major role of PPARα in regulating hepatic lipid metabolism, highlight the more modest effect of PPARα activation on gene regulation in human liver compared to mouse liver, and indicate that PPARα may have a suppressive effect on DNA synthesis in human liver. (wur.nl)
  • We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
  • In the new study, first author Albert Davis and coworkers use a mouse knockout to examine regulation of APP processing by M1. (alzforum.org)
  • Consistent with this notion, we have found that Smurf2-deficient mouse embryonic fibroblasts exhibit delayed senescence entry and enhanced potential to become immortalized in culture, while Smurf2-deficient mice show increased susceptibility to various types of cancer, including B-cell lymphoma, hepatocellular carcinoma, adenocarcinoma in small intestine and soft tissue sarcoma. (umassmed.edu)
  • In fact, Cys203 residue of Cyp-D offers been shown to play a crucial part in oxidative stress induced activation of mPTP in mouse embryonic fibroblasts (Nguyen et al. (mingsheng88.org)
  • We prospectively identified, and isolated by flow cytometry, adult mouse lateral ventricle subventricular zone (SVZ) NICs as Glast mid EGFR high PlexinB2 high CD24 −/low O4/PSA-NCAM −/low Ter119/CD45 − (GEPCOT) cells. (elifesciences.org)
  • A region of the brain called the subventricular zone contains both neural stem cells and neural progenitor cells, and is one of only two regions of the brain where neural stem cells are found in adult mammals. (elifesciences.org)
  • Swaroop explains that if scientists can understand these cues, they may eventually be able to guide the stem cells in the diseased retina of an adult eye to generate new rods. (sciencedaily.com)
  • Adult Stem Cells: The New Plastic Surgery? (scitizen.com)
  • 27 Jun, 2008 05:23 pm A new article in Live Science predicts that adult stem cells may replace silicone breast implants and other contemporary plastic surgery techniques. (scitizen.com)
  • The overall goal is to determine the cell-specific role of TGF-beta2 and its downstream mechanisms in development of congenital heart defects and adult aortic and mitral valve disease. (sc.edu)
  • The difference with Dolly is that all her DNA originated in a cell from the udder of an adult sheep. (newscientist.com)
  • And Jonathan Slack, an embryologist at the University of Bath, says that it is dangerous to base big ideas on a single case of a lamb raised from an adult ewe cell. (newscientist.com)
  • Research at the ASMG lab focuses primarily on the gut microbial composition of ruminants utilizing advanced molecular methodologies. (upenn.edu)
  • General basics of molecular cell biology are also taught, especially in the first part, while the second part focuses on translational aspects. (tum.de)
  • After successful completion of the module, students will have in-depth knowledge of cell biology and molecular biology from all aspects of tumor biology. (tum.de)
  • 2) Textbook of Molecular Cell Biology, Alberts et al. (tum.de)
  • Our laboratory uses molecular, biochemical, physiological, pharmacologic (small molecule inhibitors), cell and tissue culture, and combined optical and biomechanical engineering approaches to analyze cell/tissue/organ-specific conditional/inducible gene knockout and transgenic mouse models. (sc.edu)
  • We employ a variety of experimental systems for our studies ranging from molecular biology to transgenic and knockout mice. (stanford.edu)
  • The pig is an increasing important animal model for molecular study and pharmaceutical research. (hindawi.com)
  • The researchers turned to monkeys for more insight into the capabilities of embryonic stem cells. (livescience.com)
  • Most experiments on stem cell therapies are based on mice, and the researchers wanted to understand whether primate embryonic stem cells respond the same way as those of mice do. (livescience.com)
  • The researchers first tried creating chimeric monkeys using the process for chimeric mice. (livescience.com)
  • The researchers guessed that the culturing somehow had changed these embryonic stem cells . (livescience.com)
  • Finally, the researchers hit on a successful method, using early blastocysts that had split into no more than four separate cells. (livescience.com)
  • The researchers isolated cells from the knockout mice and tested the cells' reactions to taxanes and two other anticancer drugs, vincristine and Ara-C. Compared to cells from normal mice that still possessed the gene for Abcc10, the knockout mouse cells were much more sensitive to the drugs. (news-medical.net)
  • The goal of the Cell Culture and Cell Phenotyping Module is to streamline cell culture and phenotyping analyses for vision researchers. (utsouthwestern.edu)
  • Even though the mice do not have a well-defined macula (a region of the retina that is richer in cones than other parts of the retina), Kellogg researchers hope that this mouse model will allow them to identify the molecules needed for cone function and survival. (sciencedaily.com)
  • As part of the study, researchers also examined the effect of age on cone function in the absence of rods, recording electrical activity in the retina of Nrl knockout mice at different times after birth. (sciencedaily.com)
  • Some researchers are attempting to use stem cells to bioengineer human organs in the lab in vitro , rather than inside another species (5). (the-scientist.com)
  • 18 Sep, 2007 12:13 pm Rice University researchers have engineered musculoskeletal cartilages with human embryonic stem cells, with the hope of eventually using the neotissue. (scitizen.com)
  • 6 Jun, 2007 07:00 pm Researchers have reprogrammed skin cells into embryonic stem cells. (scitizen.com)
  • The researchers wanted to see whether "mature" cells that have differentiated to fulfil a specialised role (such as that of an udder cell or a fetal cell) could be returned to a primitive state from which they could grow into entire organisms. (newscientist.com)
  • To look at the effect in vivo, the researchers knocked out M1 in a mouse that overexpresses a mutated human APP. (alzforum.org)
  • Researchers quickly realized the value of using standardized mice strains, and the money began pouring in. (mentalfloss.com)
  • After seeing the effects on white blood cells, we decided to look at the tissue types that produce white blood cells to see if we could actually see differences there,' says Hopper-Borge. (news-medical.net)
  • Unlike some other tissues, it has not been possible to identify or purify neural stem cells directly from the tissue. (elifesciences.org)
  • We are interested in whether age-dependent accumulation of senescent cells leads to alterations in tissue microenvironment that is favorable for oncogenesis. (umassmed.edu)
  • Studies performed on numerous cancer models and tissue samples from patients shown that overexpression of CyP-A stimulates malignancy cell growth (examined in Lee and Kim, 2010). (mingsheng88.org)
  • Immunohistochemistry (Frozen) analysis of IL-10 was performed in frozen-sectioned mouse hippocampus tissue using IL-10 Polyclonal Antibody (Product # PA5-85660) at a dilution of 1:250 (Green). (thermofisher.com)
  • Project 1, Developmental Programming Associated with Maternal Diet and Obesity, research continues on Sub-objective 1A, which examines the impact of maternal high fat diet (HFD) and associated maternal obesity on offspring energy homeostasis, adipose tissue development, and metabolism. (usda.gov)
  • This research questions the contribution of lipid droplet accumulation in adipose tissue macrophages in obesityinduced inflammation and metabolic disfunction. (wur.nl)
  • 8 Jun, 2007 04:13 pm Stem cells provide the starting material for the development and repair of every organ and tissue in the body and they are present in all stages of life. (scitizen.com)
  • Mammary glands are rich in these cells, which are more adaptable than other tissue. (newscientist.com)
  • To date, a lot of EZH2 variants have been found in various cell and tissue types [ 18 - 20 ]. (hindawi.com)
  • The To specifically identify virus replication, a multiplex genome is the largest of all RNA viruses (27.6-31.2 kb), reverse transcriptase-polymerase chain reaction (RT-PCR) and the genomic RNA is infectious when transfected into was used to simultaneously amplify glyceraldehyde 3 cells from a wide variety of species and tissue types. (cdc.gov)
  • Upon and tissue tropism, illustrating the major role S-receptor entry into host cells the coronavirus gRNA (27-31.5 kb) interactions play in the species specificity and pathogene- serves as an mRNA to translate two large polyproteins (1a sis of coronaviruses. (cdc.gov)
  • Pre-GEPCOT cells could not form neurospheres but expressed the stem cell markers Slc1a3-CreER T , GFAP-CreER T2 , Sox2 CreERT2 , and Gli1 CreERT2 and were long-lived in vivo. (elifesciences.org)
  • A novel model to study in vivo PPARα activation in human liver is a chimeric mouse carrying human liver cells. (wur.nl)
  • Allan Levey and colleagues at Emory University, Atlanta, Georgia, have now used knockout mice to show that the M1 receptor is responsible for regulating APP processing in vivo. (alzforum.org)
  • They also used the enhanced gene editors on human cell lines, which demonstrated their potential for in vivo gene therapies, especially for diseases such as hemophilia, they noted. (genomeweb.com)
  • Furthermore, these data were supported by increased β-cell death in vivo in a β-cell-specific C3 knockout mouse. (lu.se)
  • In the wild-type embryo, these dead cells are rapidly engulfed and cleared by macrophages. (bris.ac.uk)
  • We show that in a macrophageless mouse embryo, null for the haemopoetic-lineage-specific transcription factor, PU.1, the task of phagocytosis is taken over by 'stand-in' mesenchymal neighbours in a clear example of cell redundancy. (bris.ac.uk)
  • In this procedure, embryonic stem cells are injected into a host embryo after they have been cultured for as long as decades. (livescience.com)
  • But the methods that work to create chimeric mice failed in rhesus monkeys, leading to offspring with cells only from the host embryo. (livescience.com)
  • So they recovered stem cells from an embryo's inner cell mass (rather than from the freezer after being cultured) and, without culturing them, injected the stem cells into a host embryo. (livescience.com)
  • We think that the developmental cues that exist in the pig will help to guide the human cells inside the porcine embryo. (the-scientist.com)
  • They produced idential lambs called Megan and Morag, which originated from different cells of the same embryo. (newscientist.com)
  • The latest experiments have also produced three lambs from the cells of a sheep fetus aborted after 26 days, and four from a nine-day-old embryo. (newscientist.com)
  • After growing and dividing for a week or so in a laboratory culture dish, the fused cell forms an early embryo called a blastocyst, which Wilmut's team implants into a surrogate mother. (newscientist.com)
  • They look like ordinary baby rhesus macaques, but Hex, Roku and Chimero are the world's first chimeric monkeys, each with cells from the genomes of as many as six rhesus monkeys. (livescience.com)
  • In contrast, pathways connected to DNA synthesis were downregulated by fenofibrate in chimeric mice with hepatocyte humanized livers yet upregulated by fenofibrate in normal mouse livers. (wur.nl)
  • Development of new transgenic and gene knock-out mice models of human diseases. (ccmb.res.in)
  • Furthermore, we constructed lncRNA Gm2044 knockout mice using the CRISPR/Cas9 method. (bvsalud.org)
  • In the current investigation, using primary human and rodent material and CRISPR/Cas9 gene-edited β-cells deficient in C3, or producing only cytosolic C3 from a noncanonical in-frame start codon, we report a protective effect of C3 against IL-1β-induced β-cell death, that is attributed to the cytosolic fraction of C3. (lu.se)
  • Optionally, a research internship (at least 6 weeks per 40 hours) in the field of tumor biology can be taken. (tum.de)
  • NIEHS research uses state-of-the-art science and technology to investigate the interplay between environmental exposures, human biology, genetics, and common diseases to help prevent disease and improve human health. (nih.gov)
  • Between 1909 and 1914, C.C. Little toiled in the biology labs of Harvard's Bussey Institute, using mice to learn how mammals inherit traits from their parents. (mentalfloss.com)
  • The incorporation of a flow cytometer and a cell sorter also reduces the cost associated with recurrent use of the main flow cytometry facility on campus. (utsouthwestern.edu)
  • have used a technique called flow cytometry to identify and isolate neural stem cells and neurosphere-forming cells directly from the subventricular zone. (elifesciences.org)
  • Here are this year's winners: Bioscience: Martin Evans, director of the school of biosciences and professor of mammalian genetics at Cardiff University, for his work in stem-cell research and the development of "knockout" mice. (lirneasia.net)
  • Although massive numbers of cells die during development, they are so rapidly phagocytosed that very few corpses are ever seen in most embryonic tissues. (bris.ac.uk)
  • The cells never fuse, but they stay together and work together to form tissues and organs," said Shoukhrat Mitalipov of the Oregon National Primate Research Center at Oregon Health & Science University. (livescience.com)
  • These stem cells will mix with the host embryo's cells to produce tissues and organs and ultimately offspring. (livescience.com)
  • Autophagy as a self-protective mechanism of cells has been widely studied and has particular physiological effects in different tissues or cells. (hindawi.com)
  • After joining the Immunopathology Unit in 1975, Dr. Bhan performed pioneering work on the application of monoclonal antibodies in the characterization of lymphoid cells in tissues and established the Diagnostic Immunoperoxidase Laboratory in 1981. (massgeneral.org)
  • This can range from the relatively pedestrian, such as a person who received a bone marrow transplant, to creatures that seem more at home in science fiction, such as animals containing cells or tissues belonging to other species. (the-scientist.com)
  • 27 Jun, 2007 06:08 pm Stem cells have the potential to become all the cells and tissues in the human body. (scitizen.com)
  • When BALB/c cells of these tissues (9). (cdc.gov)
  • Although early studies of IGSF1 ran into roadblocks and blind alleys, armed with the results of detailed clinical investigations, powerful mouse models, and new reagents, the field is now poised to discover IGSF1's function in endocrine tissues, including the pituitary and testes. (medscape.com)
  • My research interests are Mucosal Immunology and Inflammatory Bowel Disease. (massgeneral.org)
  • 3 Institute of Immunology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald - Isle of Riems, Germany. (nih.gov)
  • However, advances in stem cell research in the 2000s revolutionized the field, opening up new possibilities and new applications for multispecies organism research. (the-scientist.com)
  • 27 Nov, 2007 10:41 am Tony Maciulis talks with Dr. Jon LaPook about a breakthrough in stem cell research. (scitizen.com)
  • 21 Nov, 2007 10:54 am A huge advancement in stem cell research--and a stake in the heart of human cloning--was announced yesterday. (scitizen.com)
  • Dr. Bhan's major research interests have been the characterization of cell mediated reactions and mucosal immune responses, and defining the pathogenesis of inflammatory bowel disease. (massgeneral.org)
  • His laboratory has taken a major role in developing experimental models of inflammatory bowel disease using knockout mice. (massgeneral.org)
  • 2)In retinal cell culture, study the effects of inflammatory mediators on retinal iron transporters. (upenn.edu)
  • In diet-induced obese mice, HILPDA deficiency in macrophages markedly reduced lipid accumulation in macrophages yet it did not alter any measured inflammatory or metabolic parameters. (wur.nl)
  • However, expression of macrophage/inflammatory markers and fibrosis were not different between HILPDA knockout and floxed mice. (wur.nl)
  • 2012) Impaired Clearance and Enhanced Pulmonary Inflammatory/Fibrotic Response to Carbon Nanotubes in Myeloperoxidase-Deficient Mice. (cdc.gov)
  • A mouse line deficient in the iron transporting ferroxidases ceruloplasmin and hephaestin develops age-dependent retinal iron overload and retinal degeneration with features of AMD (Hahn et al. (upenn.edu)
  • 3) Use FACS to isolate single retinal cell types and determine the effects of iron transporter knockouts on their transcriptome. (upenn.edu)
  • During development, retinal stem cells differentiate into photoreceptors in response to external and internal cues. (sciencedaily.com)
  • The results provide the first evidence from living organisms that Abcc10 is a cell's built-in protection against the effects of powerful drugs, and raises the possibility of using Abcc10 inhibitors to break down that resistance and sensitize tumor cells to anticancer agents. (news-medical.net)
  • For that reason, Hopper-Borge thinks the best approach may be developing inhibitors that work only in tumor cells or coming up with compounds that modulate, rather than completely inhibit the protein's activity. (news-medical.net)
  • We are generating conditional Smurf2 knockout and transgenic mouse models to dissect the precise mechanism of Smurf2-mediated tumor suppression. (umassmed.edu)
  • Genomic studies have uncovered silencing TET2 mutations in B-cell and T-cell lymphomas ( 6-8 ), indicating a tumor suppressor role for TET2 in lymphoid malignancies. (aacrjournals.org)
  • As a result, there is an emergent need to explore previously unmapped mechanisms regulating distinct subpopulations of tumor cells and to understand their contribution to tumor progression and treatment response. (lu.se)
  • The presence of a subclonal population of tumor cells characterized by PDGFRβ expression was further validated in a cohort of human PanNET. (lu.se)
  • In the mouse xenograft model of colon cancer, we were able to observe a reduced tumor growth in response to CysLT1R antagonist treatment. (lu.se)
  • Also, a decreased nuclear expression of β-catenin within the epithelial tumor compartment was determined for CysLT1R mutant female mice in both models. (lu.se)
  • However, the mechanism of tumor progression in FAP/sporadic colorectal cancer and in colitis-associated colorectal cancer might differ as indicated by reduced tumor expression of COX-2 and reduced serum levels of PGE2 in the female double mutant (CysLT1R−/− ApcMin/+) mice, whereas AOM/DSS-treated female single mutant (CysLT1R−/−) mice demonstrated increased serum levels of PGE2. (lu.se)
  • 4) Mouse Models of Human Cancer. (tum.de)
  • Using genetically modified mouse models to specifically modulate senescence response in stroma, we examine the possible role of senescent microenvironment in promoting oncogenesis. (umassmed.edu)
  • Procurement and maintenance of transgenic and knock-out mouse models of human diseases where such animals are available elsewhere without restriction and provide facility and expertise to use the same in drug screening. (ccmb.res.in)
  • Breeding of currently available transgenic and knock-out mouse models. (ccmb.res.in)
  • 3) We are defining the properties of cancer stem cells that initiate and sustain the unique disease features of acute leukemias through the use of various adoptive animal models. (stanford.edu)
  • Work in knockout mouse models has shown that TET2 loss enhances hematopoietic stem cell (HSC) self-renewal, expanding the HSC pool (reviewed in refs. (aacrjournals.org)
  • In mouse models with vitamin D receptor overexpression, you actually can reduce the animal-related colitis . (medscape.com)
  • Investigations in two IGSF1 knockout mouse models converged to show that IGSF1 deficiency leads to reduced expression of the receptor for thyrotropin-releasing hormone (TRH) and impaired TRH stimulation of thyrotropin secretion, providing a candidate mechanism for the central hypothyroidism observed in patients. (medscape.com)
  • In the presented papers in this thesis we investigated the role of CysLT1R in different mouse models of colorectal cancer. (lu.se)
  • In conclusion, the presented mouse models of colorectal cancer further strengthen our previous in vitro findings and highlight the prospect of CysLT1R as an alternative therapeutic approach. (lu.se)
  • CNT in biochemical models and in cells yielding the products engineered CNT represent a possible health risk due to their that did not cause pulmonary inflammation in mice [14]. (cdc.gov)
  • Working with Swaroop, research investigator Alan J. Mears, Ph.D., deleted the gene that makes Nrl in mice, creating an Nrl-knockout strain that developed a retina without rod photoreceptors. (sciencedaily.com)
  • If a mouse possessed a trait that Little considered desirable, he'd launch a multi-generational inbreeding process to create a new strain. (mentalfloss.com)
  • These days, when geneticists want to create a new strain of mice, they often take a more hands-on approach. (mentalfloss.com)
  • TET2-knockout mice predominantly develop chronic myelomonocytic leukemia (CMML), but they can also develop other myeloproliferative as well as lymphoproliferative diseases, reflecting the prevalence of TET2 mutations in these varied hematologic malignancies. (aacrjournals.org)
  • Here we comprehensively characterized the kinetics of cell-mediated and humoral immune responses in VRP-vaccinated mice, and demonstrate that they predominantly target the nucleoprotein (NP). (cdc.gov)
  • Autophagy is a self-protective pathway of cell catabolism that allows cells to degrade misfolded proteins or damaged organelles, providing energy for cells and maintaining their homeostasis [ 10 , 11 ]. (hindawi.com)
  • Our recent studies as well as others indicate that senescent cells change their secretory patterns of cytokines, chemokines, growth factors, extracellular matrix proteins and proteases. (umassmed.edu)
  • Equipment in this laboratory includes an Attune NxT Flow Cytometer from Life Technologies and a Sony SH800 Cell Sorter. (utsouthwestern.edu)
  • Consequently, neural stem and progenitor cells have usually been studied retrospectively, based on their ability to form colonies in laboratory cell cultures. (elifesciences.org)
  • Today, it's estimated that 95 percent of the world's lab mice are descended from mice born in the Jackson Laboratory. (mentalfloss.com)
  • Induction of pre-B cells with lipopolysaccharide led to increased Oct2 levels but did not significantly increase octamer-dependent transcription in BOB.1 / OBF.1-deficient B cells. (nih.gov)
  • Here, making use of a newly generated Pdgfd knockout mouse, we reveal a functionally important malignant cell heterogeneity modulated by PDGF-DD signaling in pancreatic neuroendocrine tumors (PanNET). (lu.se)
  • OTUD3 knockout mice display nigral iron accumulation, motor deficits, and nigrostriatal dopaminergic neurodegeneration, which resembles the pathology of PD. (nature.com)
  • These drugs--originally derived from the Pacific yew tree--work by disrupting cell division, thus arresting the growth and spread of tumors. (news-medical.net)
  • He discovered, for instance, that you can breed strains of mice with bodies that readily accept transplanted cancer tumors. (mentalfloss.com)
  • The biosynthesis and storage of catecholamines in chromaffin cell tumors may differ from the biosynthesis and storage in the normal medulla. (medscape.com)
  • Functional malignant cell heterogeneity in pancreatic neuroendocrine tumors revealed by targeting of PDGF-DD. (lu.se)
  • He researched Hodgkin's disease and the role of B cells within the immune system. (wikipedia.org)
  • We previously showed that decreased IRP2 levels were observed in 1-methyl-4-phenylpyridinium (MPP + )-treated dopaminergic cells [ 25 ], supporting the physiological role of IRP2 in the control of iron balance. (nature.com)
  • In this study, we investigated the role of autophagy in glibenclamide-induced insulin secretion in pancreatic β cells. (hindawi.com)
  • Studies have shown that autophagy plays an important role in many organs, especially metabolic organs, which not only maintain the basic homeostasis of cells but also regulate cells' function [ 14 ]. (hindawi.com)
  • In the new research, Hopper-Borge and colleagues wanted to further explore, in both cultured cells and mice, the role of Abcc10. (news-medical.net)
  • These studies have demonstrated an important role of the appendix in the development of ulcerative colitis-like inflammation in mice and identified regulatory B cells capable of suppressing mucosal inflammation. (massgeneral.org)
  • While these investigations were in progress, Mears started to develop the Nrl-knockout mouse to study the role of Nrl in a living animal. (sciencedaily.com)
  • The role of TET2 and cytosine hydroxymethylation in B-cell lymphomagenesis is less known. (aacrjournals.org)
  • There's a mechanistic role in T-cell trafficking and a significant effect as it relates to the immune function. (medscape.com)
  • We also investigated the role of CysLT1R in 1) FAP/sporadic colorectal cancer by crossing ApcMin/+ mice with mice lacking CysLT1R expression and in 2) colitis-associated colorectal cancer by employing the AOM/DSS-model on mice lacking CysLT1R expression. (lu.se)
  • At high concentrations the peptide (known as P3) is toxic to neuronal cells in culture, apparently by causing calcium leakage. (alzforum.org)
  • The findings appear in the May 16, 2011 issue of the journal Cancer Research . (news-medical.net)
  • They know how to move independently and confidently between clinical application and basic scientific knowledge in the field of biomedical cancer research. (tum.de)
  • Students are able to independently evaluate current, English-language technical literature in the field of cancer research. (tum.de)
  • Successful graduates have acquired a broad knowledge to analytically structure and experimentally solve problems in the field of cancer research, supported by practical research skills and experimental-analytical knowledge from the research internship. (tum.de)
  • Ratnesh Lal, University of California, San Diego, and Ruth Nussinov, National Cancer Institute, Frederick, Maryland, present evidence that the small peptide derived from α- then γ-secretase cleavage forms ion channels in cells, reminiscent of pore-forming channels proposed for Aβ peptides. (alzforum.org)
  • In this issue of Cancer Discovery , Dominguez and colleagues report their findings on the mechanistic link between TET2 mutation and germinal center (GC) B-cell transformation (3). (aacrjournals.org)
  • These mice provided some of the first evidence that susceptibility to cancer can be inherited, just like hair color. (mentalfloss.com)
  • In 1956, he'd resigned from the lab to become the scientific director for the Tobacco Industrial Research Committee, where he argued against the idea that smoking causes lung cancer. (mentalfloss.com)
  • The development of skin sensitization is associated with, and requires, the activation and clonal expansion of allergen responsive T lymphocytes and it is these cells that orchestrate the cutaneous allergic reaction. (cdc.gov)
  • In 2001 he started working at the Center for Blood Research at Harvard Medical School, Boston, where an additional focus of his work concerns RNAi, especially microRNAs, in conjunction with immune development and control. (wikipedia.org)
  • The Cell Culture arm of this module provides technical expertise to investigators to assist in the establishment of primary cultures and development of new immortalized cell lines. (utsouthwestern.edu)
  • It consists of 4 fluorescent channels (UV to far red) and has extensive applications including bright field cell counting, wound-healing and cell growth assays, cell line development, induced pluripotent stem cell (iPSC) reprogramming, 3D invasion and migration modeling, immune-oncology, virology, and fluorescent-based assays. (utsouthwestern.edu)
  • As scientific understanding of stem cells, gene editing, and organism development improved, Garry felt that her career path was clear. (the-scientist.com)
  • Dr. Xinyu Zhao of the Waisman Center and Department of Neuroscience at University of Wisconsin-Madison joins us in this seminar to present Interrogate the Functions of FMRP in Brain Development Using Stem Cells. (fraxa.org)
  • My research focus is on gaining an understanding of the unique and redundant biological function and properties of the three transforming growth factor beta (TGFβ) ligands and their signaling mechanisms in cardiovascular development and homeostasis and in disease pathogenesis. (sc.edu)
  • The major goals of this project are to define cell type specific function and mechanisms of TGF-beta2 in development and progression of thoracic aortic aneurysm and dissection. (sc.edu)
  • EZH2, a methyltransferase catalyzing H3K27me3, has been abundantly studied in human and mouse embryonic development. (hindawi.com)
  • In the fifth week of fetal development, neuroblastic cells migrate from the thoracic neural crest to form the sympathetic chains and preaortic ganglia. (medscape.com)
  • Although the follicular development and fertility of female lncRNA Gm2044 knockout mice were not affected, the serum estradiol concentration in female lncRNA Gm2044 knockout mice significantly decreased. (bvsalud.org)
  • Consistently, decreased levels of OTUD3 are detected in transgenic PD mice expressing A53T mutant of human α-synuclein. (nature.com)
  • β cells are the targets of glibenclamide and the only highly differentiated insulin-secreting cell in the human body, which secrete a certain amount of insulin to maintain glucose homeostasis [ 9 ]. (hindawi.com)
  • To date, most research has centered on rods, in part because they make up almost 95% of the photoreceptors in human retina. (sciencedaily.com)
  • Animals with human cells could provide donor organs or help us understand neuropsychiatric disorders. (the-scientist.com)
  • Other scientists, including Jun Wu, a stem cell biologist at the University of Texas Southwestern Medical Center, are also studying chimeras with the ultimate goal of one day being able to grow enough human organs to meet the enormous need for transplants, potentially saving hundreds of thousands of lives. (the-scientist.com)
  • Model systems to study PPARα in human liver vary from hepatoma cell lines, human primary hepatocytes, human precision cut liver slices, and mice expressing human PPARα. (wur.nl)
  • These mice are generated by transplanting human hepatocytes into albumin enhancer-driven urokinase-type plasminogen activator transgenic/severe combined immunodeficiency (uPA/SCID) mice, leading to replacement of the host hepatocytes. (wur.nl)
  • Fenofibrate increased the size of the mouse but not human hepatocytes and tended to reduce steatosis in the human hepatocytes. (wur.nl)
  • Quantitative PCR indicated that induction of PPARα targets by fenofibrate was less pronounced in the human hepatocytes than in the residual mouse hepatocytes. (wur.nl)
  • 6 Sep, 2007 12:57 pm British authorities decided yesterday to permit research that uses animal eggs to create human stem cells because of the limited supply of human eggs. (scitizen.com)
  • This led to the creation of "knockout" mice, which are used to model human diseases by deactivating a specific gene. (lirneasia.net)
  • Human and animal coronaviruses are transmitted by the drome-associated coronavirus (SARS-CoV) will greatly respiratory or enteric routes and initially infect epithelial advance research on this emerging virus. (cdc.gov)
  • Several possible mechanisms were considered for the induction of human leukemia, such as clastogenic damage to circulatory stem cells. (who.int)
  • To circumvent these obstacles, we have developed two methods for the investigation of human neural cells in culture. (lu.se)
  • Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. (thermofisher.com)
  • IL-1β is a proinflammatory cytokine which detrimentally affects β-cell function. (lu.se)
  • Here, we show that BNF treatment markedly inhibits liver mitochondrial O 2 consumption rate (OCR), ADP-dependent OCR, and also reserve OCR, in wild-type mice but not in Cyp1a1/1a2(-/-) double knockout mice. (uaeu.ac.ae)
  • First, they extract immature, unfertilised egg cells called oocytes from the ovaries of ewes. (newscientist.com)
  • Neurosphere formation is commonly used as a surrogate for neural stem cell (NSC) function but the relationship between neurosphere-initiating cells (NICs) and NSCs remains unclear. (elifesciences.org)
  • Neural stem cells have long lives, much of which they spend in a quiescent state. (elifesciences.org)
  • However, the exact relationship between neural stem cells and neurosphere-forming cells has been uncertain. (elifesciences.org)
  • The neural stem cells, which Mich, Signer et al. (elifesciences.org)
  • This high throughput drug screen uses neural stem cells from Fragile X knockout mice to identify small molecules which may be therapeutic in Fragile X. (fraxa.org)
  • With a $50,000 grant from FRAXA Research Foundation, Dr. Clive Svendsen and his team at the University of Wisconsin grew neural stem cells that expressed the Fragile X mutation to help scientists better understand the gene characteristics. (fraxa.org)
  • Beneficial effect on dendrites in neural cells was detectable already before any aggregates or fibrils were detectable. (lu.se)
  • A cell culture model, based on wild-type and specific integrin knockout cell lines lacking the integrin subunits αv, β1 or β3, was used to investigate the susceptibility to WNV, and to evaluate binding and replication efficiencies of four distinct strains (New York 1999, Uganda 1937, Sarafend and Dakar). (nih.gov)
  • Similarly, in diet-induced NASH mice, HILPDA hepatocyte deficiency modestly yet significantly reduced liver triglyceride accumulation and plasma ALT levels. (wur.nl)
  • Comparison between WT and Opn KO mice revealed that OPN critically regulated MWCNT-induced lung fibrosis as indicated by reduced fibrotic focus formation and myofibroblast accumulation in Opn KO lungs. (cdc.gov)
  • Cell Signal (2020 Oct) 74:109701. (nih.gov)
  • 1995). Latest studies on individual SH-SY5Y neuroblastoma cells showed that CyP-D may also become a redox sensor in mitochondria of mammalian cells (Linard et al. (mingsheng88.org)
  • Previous studies in suckling mice inoculated intracranially or intraperitoneally suggest that mice are not permissive to SARS-CoV (1,8). (cdc.gov)