• In mammalian cells, active and silent rDNA promoters are characterized by specific histone modifications, DNA methylation, and positioning of the promoter-bound nucleosome (reviewed in reference 22 ). (asm.org)
  • Using a CRISPR Cas9-guided immunoprecipitation assay, we identify a circular RNA in the FLI1 promoter chromatin complex, consisting of FLI1 exons 4-2-3, referred to as FECR1.Overexpression of FECR1 enhances invasiveness of MDA-MB231 breast cancer cells. (biomedcentral.com)
  • Using this CasIP assay, we identified FECR1, a FLI1 exonic circular RNA that binds to the FLI1 promoter and epigenetically activates FLI1 in breast cancer cells. (biomedcentral.com)
  • PB514B-1) drives expression of your gene-of-interest from the strong CMV promoter and expression of both an RFP reporter and puromycin resistance from the EF1α promoter. (reportergene.com)
  • Pre-made lentivirus express RFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. (reportergene.com)
  • This lentivirus also contain the puromycin selection marker under RSV promoter. (reportergene.com)
  • GenTargetDescription:Concentrated lentivirus in PBS, expressing human TERT gene under suCMV promoter, containing Puromycin marker.Storage. (reportergene.com)
  • In addition, transgenes inserted in order to be expressed from either the native PPP1R12C or an exogenous promoter displayed consistent levels of expression over many cell divisions. (genecopoeia.com)
  • Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. (sigmaaldrich.com)
  • Ectopic expression of AUF1 also promoted stemness in mammary epithelial cells, and thereby increased the proportion of cancer stem cells. (nature.com)
  • BFP expression in A549-Cas9-Blast stable cells infected with arrayed CRISPR lenti-gRNA viruses, seven days post infection, 20X magnification. (sigmaaldrich.com)
  • We also find that expression of CK1ε is able to promote oncogenic transformation of human cells in a β-catenin-dependent manner. (plos.org)
  • In non-small cell lung cancer, the FLI1 expression score is associated with the stage of SCLC. (biomedcentral.com)
  • After Foxc1 was overexpressed, the cognitive dysfunction of mice that underwent CLP surgery was improved, with the expression of IκBα also increased, microglial cell migration, the expression of p65, IL‑1β and TNF‑α and neuronal apoptosis were all decreased in vivo and in vitro , which were in turn reversed by the inhibition of IκBα in vitro . (spandidos-publications.com)
  • We show here that expression of EBF3 resulted in cell cycle arrest and apoptosis. (aacrjournals.org)
  • The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
  • Metastatic spread is responsible for the majority of cancer-associated deaths and is affected by a variety of factors, including the deregulation of gene expression, signal transduction, and epigenetic modification, as well as epithelial-to-mesenchymal transition (EMT) and the transformation of tumor cells into stem/progenitor cells. (jcancer.org)
  • By combining in situ hybridization with immunohistochemistry, alpha 1(III) mRNA expression was colocalized to cells that labeled for alpha-smooth muscle actin identifying them as myofibroblasts. (nih.gov)
  • Interestingly, the major site of alpha 1(III) mRNA expression shifted to tubuloepithelial cells with the waning of myofibroblast infiltration on day 21. (nih.gov)
  • alpha 1(III) mRNA expression also localized to cells that labeled for alpha-smooth muscle actin. (nih.gov)
  • The purpose of this study was to determine how expression of Lcn2 is regulated in fat cells and to ascertain whether Lcn2 could be involved in metabolic dysregulation associated with obesity. (diabetesjournals.org)
  • The Super PiggyBac Transposase is delivered to the cell via the Super PiggyBac Transposase Expression Vector, which is co-transfected with one or more PiggyBac Vectors. (reportergene.com)
  • sgRNA-expressing cells were selected for 7 days with puromycin (Puro) and Cas9 expression induced with doxycycline (Dox) for 9 days. (medicalxpress.com)
  • We also found that SRC-3 depletion in cultured HESCs results in a significant attenuation in the induction of a wide-range of established biomarkers of decidualization, despite exposure of these cells to a deciduogenic stimulus and normal progesterone receptor expression. (frontiersin.org)
  • thus, Pnn may affect crucial alternative splicing (AS) decisions and, in turn, impact cell-type specific gene expression. (molvis.org)
  • RPE1-Cas9i-mt-mKeima cells were transduced with a lentiviral CRISPR sgRNA library targeting 606 E3 ligases. (medicalxpress.com)
  • Puromycin-sensitive amino peptidase also known as cytosol alanyl aminopeptidase or alanine aminopeptidase (AAP) (EC 3.4.11.14) is an enzyme that in humans is encoded by the NPEPPS gene. (wikipedia.org)
  • This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. (wikipedia.org)
  • HCT-116 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) and K562 cells were cultured in RPMI 1640 Medium supplemented, both supplemented with 10% fetal bovine serum (FBS) and 1% of either penicillin-streptomycin or antibiotic-antimycotic. (nih.gov)
  • Gold Biotechnology offers more than 200 unique antibiotic agents for use in a wide variety of cell culture, microbiology and molecular biology applications. (goldbio.com)
  • These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)
  • Silencing of the EBF3 locus was observed in brain, colorectal, breast, liver, and bone tumor cell lines and its reactivation was achieved on treatment with 5-aza-2′-deoxycytidine and trichostatin A in a significant portion of these tumor cells. (aacrjournals.org)
  • Since epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors, including gefitinib (GEF) have been reported to induce the apoptosis of several cancer cell lines, in the present study, we examined whether the cytotoxic effects of GEF are further enhanced under amino acid starvation (AAS) culture conditions. (spandidos-publications.com)
  • SPIN1 ablation activates p53, suppresses cell growth, reduces clonogenic ability, and induces apoptosis of human cancer cells. (elifesciences.org)
  • Lipopolysaccharide (LPS) treatment was found to trigger BV‑2 cell migration, inflammation and neuronal apoptosis. (spandidos-publications.com)
  • Our data indicate that plakoglobin promotes tumor cluster formation in IMPC and downregulates apoptosis in the cell clusters through activation of PI3K/Akt/Bcl-2 signaling. (jcancer.org)
  • In previous reports, we identified a set of genes regulated by Hh signalling in ovarian tumour cells among which MMP-7 was identified as a potential Hh target gene candidate. (jcancer.org)
  • Here we identify CSNK1E , the gene encoding casein kinase 1 epsilon (CK1ε) as required specifically for the proliferation of breast cancer cells with activated β-catenin and confirm its role as a positive regulator of β-catenin-driven transcription. (plos.org)
  • The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
  • Scientists at the University of Liverpool have figured out how mutations in a gene called FBXL4 can lead to an excess of mitophagy-the disposal of mitochondria, the 'power stations' within nearly all human cells. (medicalxpress.com)
  • Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)
  • HepG2 cells were used to further explore the underlying mechanisms in vitro . (karger.com)
  • Indeed, Hbp1 accumulates in cells lacking GID/CTLH activity, and Hbp1 physically interacts and is ubiquitinated in vitro by reconstituted GID/CTLH complexes. (elifesciences.org)
  • A microglia‑mediated inflammation model was induced by LPS in BV‑2 microglial cells in vitro , whilst a SAE‑related cognitive impairment model was established in mice using cecal ligation and perforation (CLP) surgery. (spandidos-publications.com)
  • Both in vitro and in vivo data showed overexpression of plakoglobin in both the cell membrane and the cytoplasm of IMPC clusters. (jcancer.org)
  • RESULTS- Lcn2 is highly expressed by fat cells in vivo and in vitro. (diabetesjournals.org)
  • In this study, we focused on genes of sphingolipid metabolism and investigated their roles in PrS formation using an in vitro mouse ES cell differentiation system. (bvsalud.org)
  • The HaCaT human keratinocytes (provided by RWTH, Aachen, Germany) were genetically modified at the Institute of Anatomy and Cell Biology of the RWTH, Aachen (laboratory of CJ Wruck). (europa.eu)
  • The Wnt/β-catenin pathway plays a critical role in embryonic development, maintenance of multipotent progenitor cell populations and proliferation of many tissue types [1] , [2] . (plos.org)
  • Deletions of multiple GID subunits compromise cell proliferation, and this defect is accompanied by deregulation of critical cell cycle markers such as the retinoblastoma (Rb) tumor suppressor, phospho-Histone H3 and Cyclin A. We identify the negative regulator of pro-proliferative genes Hbp1 as a bonafide GID/CTLH proteolytic substrate. (elifesciences.org)
  • Moreover, AUF1 knockdown sensitized breast cancer cells to the killing effect of cisplatin. (nature.com)
  • Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)
  • Knockdown of PNN in HCET cells led to specific changes in the inclusion of multiple cassette exons as well as in the use of alternative splice sites in RP11-322M19.1 and RP11-18I14.1, resulting in considerable net changes in the ratio between the splice variants. (molvis.org)
  • Here we extend those findings to humans using only genetically unmodified human naive embryonic stem cells (cultured in human enhanced naive stem cell medium conditions) 4 . (nature.com)
  • Human wild-type HeLa cell line ( ab255928 ). (abcam.com)
  • We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)
  • When expressed in ciliated hTERT-RPE1 human cell line, P/rd is localized to cilia. (jneurosci.org)
  • The LuSens cell line is an immortalised adherent cell line derived from HaCaT human keratinocytes stably transfected with a selectable plasmid. (europa.eu)
  • We previously demonstrated the critical involvement of SRC-2 in murine embryo implantation as well as in human endometrial stromal cell (HESC) decidualization, a cellular transformation process required for trophoblast invasion and ultimately placentation. (frontiersin.org)
  • Finally, in situ hybridization analyses revealed the presence of RP11-295G20.2 in the nuclei of corneal epithelial cells, but not in the stromal cells of the human cornea, while RP11-322M19.1 was present in epithelial and non-epithelial cells. (molvis.org)
  • Later, an analogous Safe Harbor site for transgene insertion was described for human cells (DeKelver, et al. (genecopoeia.com)
  • As experts in innate immunity and cell culture, we offer a large selection of products including PRR ligands , cell lines , selective antibiotics , antimicrobial agents , vaccine adjuvants , and antibodies . (invivogen.com)
  • All cells were allowed to recover for 24 hours before undergoing puromycin selection. (nih.gov)
  • It can be used as a cell selection agent. (goldbio.com)
  • NPEPPS is induced in neurons expressing mutant huntingtin and is critical in preventing the accumulation of polyglutamine in normal cells. (wikipedia.org)
  • demonstrate through studies on induced pluripotent stem cell-derived cortical neurons that miR-33a is a potential therapeutic target for the treatment of SPG4-related hereditary spastic paraplegia. (portlandpress.com)
  • In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. (sigmaaldrich.com)
  • Macroautophagy (thereafter designated as autophagy) is a self-digestive system conserved in all eukaryotic cells. (spandidos-publications.com)
  • We have shown that AUF1 induces EMT and stemness in breast epithelial cells via stabilization of the SNAIL1 and TWIST1 mRNAs, and their consequent upregulation. (nature.com)
  • These data prompted us to ask whether AUF1 upregulation could promote EMT as well as stemness in mammary epithelial cells. (nature.com)
  • Autophagy is an intracellular degradation system that ensures a dynamic recycling of a variety of building blocks required for self-renewal, homeostasis, and cell survival under stress. (hindawi.com)
  • Matrix metalloproteinase-7 (MMP-7) facilitates degradation of the extracellular matrix, promoting the invasion of tumour cells, and is associated with cancer progression and metastasis. (jcancer.org)
  • Electron microscopy demonstrated that the CAL 27 cells treated with GEF under AAS culture conditions exhibited swelling of the cytosol and organelles with an increased number of autophagosomes and autolysosomes, but without chromatin condensation and nuclear fragmentation. (spandidos-publications.com)
  • 13 Genome Plasticity and B Cell, University of Paris-Sud, Centre National de la Recherche Scientifique, Cancer Institute Gustave Roussy, Villejuif, France. (jci.org)
  • Mouse naive embryonic stem cells have recently been shown to give rise to embryonic and extra-embryonic stem cells capable of self-assembling into post-gastrulation structured stem-cell-based embryo models with spatially organized morphogenesis (called SEMs) 3 . (nature.com)
  • These include embryonic disc and bilaminar disc formation, epiblast lumenogenesis, polarized amniogenesis, anterior-posterior symmetry breaking, primordial germ-cell specification, polarized yolk sac with visceral and parietal endoderm formation, extra-embryonic mesoderm expansion that defines a chorionic cavity and a connecting stalk, and a trophoblast-surrounding compartment demonstrating syncytium and lacunae formation. (nature.com)
  • Much of this process relies on the morphogenesis of the extra-embryonic tissues and the effect this has on the organization of embryonic cells. (nature.com)
  • AAV insertions at this locus, also known as AAVS1, do not have any visible phenotype on cells, including primary and immortalized cells, induced pluripotent stem cells (iPSC), and embryonic stem cells. (genecopoeia.com)
  • Generation of UL128-shRNA transduced fibroblasts for the release of cell-free. (invivogen.com)
  • Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. (deepdyve.com)
  • The ability to introduce transgenes into biological systems, such as cultured cells or animals, is of fundamental importance in biology and medicine. (genecopoeia.com)
  • Basal macroautophagy (thereafter referred to as autophagy), a catabolic recycling system in cells, is key to maintaining cellular homeostasis and survival. (hindawi.com)
  • While the importance of autophagy for cell homeostasis and survival has long been appreciated, its role in tumorigenesis and cancer progression is still developing [ 10 , 11 ]. (hindawi.com)
  • Autophagic cell death was excluded as the inhibition of autophagy did not attenuate the cytotoxicity. (spandidos-publications.com)
  • Cellular proteostasis involves the coordinated and compensatory action of pathways that control biogenesis, folding, trafficking and breakdown of proteins allowing the cell to adapt to physiological or pathological environmental changes. (elifesciences.org)
  • First, the protein cleaves several other proteins called cytokine receptors on the surface of cells. (medlineplus.gov)
  • These peptides are exported to the cell surface, where they attach to major histocompatibility complex (MHC) class I proteins. (medlineplus.gov)
  • Zhang H, Wang Y, Chen T, Zhang Y, Xu R, Wang W, Cheng M, Chen Q. Aberrant Activation Of Hedgehog Signalling Promotes Cell Migration And Invasion Via Matrix Metalloproteinase-7 In Ovarian Cancer Cells. (jcancer.org)
  • Using mouse models, we validated the metastatic potential of tumor clusters was higher than single cells in vivo . (jcancer.org)
  • We, therefore, propose that CFU-L can be used to quantify the number of "hepatocytes that engraft and proliferate in vivo" as a quantitative assay for stem cells that utilize colony-forming ability, similar to that observed in hematopoietic stem cells. (bvsalud.org)
  • Overexpression of FLI1 in erythroblasts causes inhibition of differentiation and ultimately the development of pre-T cell lymphoblastic leukemia/lymphoma [ 11 ]. (biomedcentral.com)
  • If the immune system recognizes the peptides as foreign (such as viral or bacterial peptides), it responds by triggering the infected cell to self-destruct. (medlineplus.gov)
  • In this study, we hence aimed to develop a new method to monitor autophagic flux using small cell-permeable fluorescent probes. (bvsalud.org)
  • Although the LAT family plays important roles in the development and function of normal tissues, they are frequently increased in cancer cells ( 3 ). (spandidos-publications.com)
  • A detrimental role for oestrogens in FPAH is supported by studies that show oestrogen is a potent mitogen of pulmonary vascular smooth muscle cells 4 . (ersjournals.com)
  • The invasive and migratory capabilities of tumour cells were tested with the transwell and wound healing assays, respectively. (jcancer.org)
  • We reported here that plakoglobin, as a key component in cell adhesion, can promote collective metastasis through facilitating IMPC clusters formation. (jcancer.org)
  • All seeding densities should be based on cell counts gained by established methods. (abcam.com)
  • MISSION ® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines. (sigmaaldrich.com)
  • Under AAS culture conditions, the cell killing effect of GEF was synergistically pronounced in the EGFR-expressing cell lines, namely, CAL 27, Detroit 562, A549 and PANC-1 cells compared with those treated with either GEF or AAS alone. (spandidos-publications.com)
  • To uncover genetic dependencies in breast cancer cells that harbor active β-catenin signaling, we performed RNAi-based loss-of-function screens in breast cancer cell lines in which we had characterized β-catenin activity. (plos.org)
  • NPEPPS has been proposed to function in a variety of processes, including metabolism of neuropeptidase, regulation of the cell cycle, and hydrolysis of proteasomal products to amino acids. (wikipedia.org)
  • Together, these findings provide clear evidence that AUF1 is an important inducer of the EMT process through stabilization of SNAIL1 and TWIST1 and the consequent promotion of breast cancer stem cells. (nature.com)
  • As an extension of the phenomenon observed in hematopoietic stem cells giving rise to colonies within the spleen, commonly referred to as "colony-forming units in spleen (CFU-s)", we hypothesize that "colony-forming units in liver (CFU-L)" serves as a reliable indicator of stemness, engraftment, and functionality of hepatocytes. (bvsalud.org)
  • The addition of essential amino acids, but not non-essential amino acids to the cell culture medium resulted in the cancellation of this pronounced cytotoxicity. (spandidos-publications.com)
  • The transport of serum amino acids into cells is an active process that is facilitated by plasma membrane-localized amino acid transporters. (spandidos-publications.com)
  • The members of the L-type amino acid transporter (LAT) family are Na + -independent transporters that deliver neutral amino acids into cells ( 2 ). (spandidos-publications.com)
  • We will provide viable cells that proliferate on revival. (abcam.com)
  • Invading cancer cells can utilize different modes of migration including single cell migration and collective migration. (jcancer.org)
  • The sensory primary cilium of vertebrate photoreceptor cells houses thousands of photosensitive disk membranes that are renewed continuously throughout our lifespan. (jneurosci.org)
  • Despite the importance of tubulointerstitial fibrosis as a predictor of renal function in patients with primary glomerular disease, the identity of the cell(s) that is the source of interstitial collagen production remains unknown. (nih.gov)
  • 2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. (abcam.com)
  • 3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. (abcam.com)
  • Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x10 4 cells/cm 2 . (abcam.com)
  • R.I. FRESHNEY: "Culture of Animal Cells: A Manual of Basic Technique", 1987, ALAN R. LISS, INC. (sumobrain.com)
  • Stocks of the LuSens cell line are stored in liquid nitrogen in the cell bank of ICCR-Roßdorf GmbH (aliquots of cells in freezing medium at 1.5-5 × 106 cells/cryovial) allowing the repeated use of the same cell culture batch in experiments. (europa.eu)
  • The confluent cells will be incubated at 37 ± 1.5 °C and 5.0 ± 0.5 % CO2 (standard cell culture conditions). (europa.eu)
  • Length-Dependent Modulation of B Cell Activating Factor Transcripts in Chicke. (invivogen.com)
  • To amplify self-reporting transcripts from single cell RNA-seq libraries, we took 9 µl of RT product (the other half was kept in reserve) and added it to 25 µl Kapa HiFi HotStart ReadyMix and 15 µl ddH2O. (nih.gov)
  • Our biochemical and cellular analysis thus demonstrates that the GID/CTLH complex prevents cell cycle exit in G1, at least in part by degrading Hbp1. (elifesciences.org)
  • These molecular findings are supported at the cellular level by the inability of HESCs to morphologically transform from a stromal fibroblastoid cell to an epithelioid decidual cell when endogenous SRC-3 levels are markedly reduced. (frontiersin.org)
  • MMP-7 facilitated the invasion and migration of ovarian tumour cells, indicating its key function in ovarian cancer progression. (jcancer.org)
  • Forkhead box C1 (Foxc1) is a member of the forkhead transcription factor family that has been found to regulate in cell migration. (spandidos-publications.com)
  • Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1. (abcam.com)
  • One classical method for stably integrating transgenes into cells is to transfect or inject linearized DNA and allow random, non-specific integration. (genecopoeia.com)