• The major function of IL-3 cytokine is to regulate the concentrations of various blood-cell types. (wikipedia.org)
  • Interleukin 3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. (wikipedia.org)
  • The cytokine was originally discovered via the observation that it induced the synthesis of 20alpha-hydroxysteroid dehydrogenase in hematopoietic cells and termed it interleukin-3 (IL-3). (wikipedia.org)
  • Interleukin-9 is required for allergic airway inflammation mediated by the cytokine TSLP. (nature.com)
  • This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. (nih.gov)
  • Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. (nih.gov)
  • This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. (nih.gov)
  • This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. (nih.gov)
  • This active form of vitamin D can inhibit immune cell proliferation, promote an anti-inflammatory cytokine profile, expand regulatory T cells, enhance glucocorticoid actions, increase glutathione production, and inhibit hepatic stellate cells. (springer.com)
  • Identification and purification of natural killer cell stimulatory factor (NKSF), a cytokine with multiple biologic effects on human lymphocytes. (nature.com)
  • 16 Such observations have initiated the idea that HLA-G might protect tissues from damage by infiltrating cytotoxic T cells during the process of inflammation 2 because HLA-G has been shown to modulate the T helper (Th) cytokine balance in favour of Th2 type anti-inflammatory cytokine responses. (bmj.com)
  • The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. (creativebiomart.net)
  • Interleukin-33 (IL-33) is a recently identified cytokine, an important member of the interleukin-1 family. (frontiersin.org)
  • These helper T cells make a cytokine called IL-17 that causes inflammation. (nih.gov)
  • Gene expression analysis found that Th17 cells from TGF-beta cultures had higher levels of an anti-inflammatory cytokine called IL-10. (nih.gov)
  • We did not observe any difference between mast-cell-deficient and control mice with regard to weight loss, bacterial clearance, inflammation or cytokine production. (uni-koeln.de)
  • The resulting complex inhibits phosphatase calcineurin, thus blocking T-cell activation and cytokine release. (medscape.com)
  • Methods We stimulated purified monocytes and whole peripheral blood mononuclear cells (PBMCs) from patients with GPA, patients with MPA or healthy controls with PR3 or MPO and visualised MGC and granuloma-like structure formation using light, confocal and electron microscopy, as well as measuring the cell cytokine production. (bmj.com)
  • In the second report [3], mouse bone marrow cells marked with green fluorescent protein were found to fuse with ES cells in culture in the presence of the cytokine, interleukin-3, which is known to encourage cell fusion. (i-sis.org.uk)
  • Secondary eosinophilia is a reactive phenomenon driven by eosinophilopoietic cytokine release by nonmyeloid cells. (medscape.com)
  • There's an anti-inflammatory response with cytokine interleukin-10. (medscape.com)
  • IL-3 shares the β subunit with IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF). (wikipedia.org)
  • Besides IL-3, granulocyte-macrophage colony-stimulating factor supported the growth of the KMT-2 cells, but IL-1 alpha, IL-2, IL-4, IL-5, and erythropoietin did not. (ashpublications.org)
  • Neutrophils activated by granulocyte-macrophage colony-stimulating factor express receptors for interleukin-3 which mediate class II expression. (bdbiosciences.com)
  • It also inhibits transcription for genes that encode interleukin (IL)-3, IL-4, IL-5, granulocyte-macrophage colony-stimulating factor (GM-CSF), and tumor necrosis factor-alpha, all of which are involved in early stages of T-cell activation. (medscape.com)
  • The primary stimuli for eosinophil production are interleukin (IL)-5, IL-3, and the granulocyte-macrophage colony-stimulating factor (GM-CSF). (medscape.com)
  • Eosinophil production appears to be regulated by T cells through the secretion of the hematopoietic growth factors granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and interleukin-5 (IL-5). (msdmanuals.com)
  • Interleukin-3 receptor (IL-3 R) is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (IL-3 Ralpha or CD123) and a 120-140 kDa IL-3 R beta subunit (IL-3 Rbeta or CD131). (watson-bio.cn)
  • The alpha subunit of the IL3 receptor binds IL 3 with low affinity. (reactome.org)
  • Furthermore, HLA-G expression could not be detected in cultured patient TFCs under basal conditions or after stimulation with the proinflammatory cytokines-interleukin 1α, interferon γ, and tumour necrosis factor α. (bmj.com)
  • changes in expression of the immunomodulating cytokines interleukin (IL)-1 beta, IL-6, and IL-8. (nih.gov)
  • Past studies have found that a combination of 3 cytokines-interleukin-1-beta (IL-1-beta), interleukin-6 (IL-6) and transforming growth factor beta (TGF-beta)-drive Th17 cell development. (nih.gov)
  • Determine whether this regimen can induce cell apoptosis in these patients. (knowcancer.com)
  • 6 With respect to activity, HLA-G molecules can inhibit natural killer cell mediated and antigen specific CD8 + T cell mediated cytolysis, 7 induce apoptosis of activated CD8 + T cells, 8 suppress the proliferation of allogeneic CD4 + T cells, 9 and inhibit the transendothelial migration of natural killer cells. (bmj.com)
  • Ectopic Mcl-1 expression attenuates Bak activation and apoptosis by ABT-737 + roscovitine, whereas cells overexpressing Bcl-2 or Bcl-xL remain fully sensitive. (aacrjournals.org)
  • HA14-1) that disable Bcl-2, resulting in induction of apoptosis in leukemia cell lines ( 8 ). (aacrjournals.org)
  • induction of apoptosis, and (3). (nih.gov)
  • Flow cytometric analysis revealed partial cell arrest in the G(1) phase at less than 5 micro L/mL and induction of apoptosis at 10 micro L/mL or more of ethanol and water extracts, with the latter exhibiting more pronounced inhibition than the former. (nih.gov)
  • I'm-Yunity acts selectively in HL-60 leukemic cells, resulting in cell cycle restriction through the G(1)/S checkpoint and the induction of apoptosis. (nih.gov)
  • Apoptosis is a genetically programmed process for the elimination of damaged or redundant cells by activation of caspases (aspartate-specific cysteine proteases). (kegg.jp)
  • It is the balance between the pro-apoptotic and anti-apoptotic signals that eventually determines whether cells will undergo apoptosis, survive or proliferate. (kegg.jp)
  • Caspases are a family of calcium -dependent cysteine proteases , which play essential roles in apoptosis (programmed cell death), necrosis and inflammation . (wikidoc.org)
  • Caspases are essential in cells for apoptosis , one of the main types of programmed cell death in development and most other stages of adult life, and have been termed "executioner" proteins for their roles in the cell. (wikidoc.org)
  • CASP14 , is not involved in apoptosis or inflammation, but instead is involved in skin cell development. (wikidoc.org)
  • The importance of caspases to apoptosis and programmed cell death was originally established by Robert Horvitz and colleagues who found that the ced-3 gene was required for the cell death that took place during the development of the nematode C. elegans . (wikidoc.org)
  • thus, those cells that fail to receive a signal (ie, do not recognize self) die by apoptosis or become inactive. (medscape.com)
  • Besides, the levels of apoptosis (caspase 3, Bax/Bcl2 ratio) and autophagy markers (Sirt1, Beclin-1, and LC3 II/I ratio) as well as the expression of miR-144 and miR-34a in cortex tissue were determined. (bvsalud.org)
  • Over the past 2 decades, substantial progress has been made in understanding the mechanisms of eosinophil production, eosinophil programmed cell death (apoptosis), and how eosinophil immunology contributes to both host defenses against infections and to tissue damage within the host in cases of allergic and autoimmune diseases. (medscape.com)
  • Signaling triggered by OSM appears to play a role in many body processes, including the development of blood cells, the maturation of cells to become certain cell types, an immune system response called inflammation, and the self-destruction (apoptosis) of cells. (medlineplus.gov)
  • In lichen and macular amyloidosis, it is thought that scratching the itchy skin causes skin cells to undergo apoptosis, releasing proteins that abnormally clump together and form amyloids. (medlineplus.gov)
  • Researchers speculate that a reduction of signaling triggered by OSM may make cells more likely to undergo apoptosis, which may promote amyloid buildup. (medlineplus.gov)
  • Interleukin 4 induces apoptosis of acute myeloid leukemia cells in a Stat6-dependent manner. (lu.se)
  • IL-3 receptors can be found on a variety of cell types including many immature myelomonocytic cells in the hemopoietic system such as hemopoietic progenitor cells, as well as certain myeloid progenitors, basophils, and eosinophils. (wikipedia.org)
  • Binding studies with 125I-labeled recombinant human (rh) IL-3 indicated that IL- 3 bound to a single class of high affinity receptors (approximately 4,000 receptors/cell) on KMT-2 cells with a kd of approximately 200 pmol/L. The chemical cross-linking assay demonstrated that radiolabeled hIL-3 bound three molecules with molecular masses of 170, 130, and 70 Kd. (ashpublications.org)
  • Present data suggest that the newly established human cell line will be a valuable tool for the biologic assay of hIL-3, and a model for biochemical studies of IL-3 receptors. (ashpublications.org)
  • Vitamin D receptors are expressed on the principal cell populations involved in the innate and adaptive immune responses. (springer.com)
  • a partial map of receptors on human mast cells cultured in vitro. (bdbiosciences.com)
  • As the infection progresses, SARS-CoV-2 becomes engulfed in human cells, reducing the number of ACE2 receptors on a cell and leading to an increase of angiotensin II in the blood. (neurosciencenews.com)
  • The 'extrinsic' pathway involves stimulation of members of the tumor necrosis factor (TNF) receptor subfamily, such as TNFRI, CD95/Fas or TRAILR (death receptors), located at the cell surface, by their specific ligands, such as TNF-alpha, FasL or TRAIL, respectively. (kegg.jp)
  • They used the ability of Ba/F3 lymphoid cells to overcome their dependence on interleukin 3 with activated epidermal growth factor receptors, or EGFRs, which are perennially turned on in many cancers. (asbmb.org)
  • It induces proliferation and differentiation in both early pluripotent stem cells and committed progenitors. (wikipedia.org)
  • IL-3 also induces various effector functions in both immature and mature cells that more precisely modulate the body's defense against microbial pathogens. (wikipedia.org)
  • IL-3/Receptor complex induces JAK2/STAT5 cell signalization pathway. (wikipedia.org)
  • IL-3 also induces histamine release from mature mast cells during proliferation and activation. (fujifilm.com)
  • Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (T h 1)-specific immune responses and inhibits the development of IL-4-producing Th cells. (nature.com)
  • IL-33 induces T cells to produce IL-4, IL-5, and IL-13, and potently induces pro-inflammatory cytokines and chemokines through a Th2-dependent pathway, and also promotes Th1-type responses ( 5 ). (frontiersin.org)
  • Although long-term repopulating hematopoietic stem cells (HSC) can self-renew and expand extensively in vivo, most efforts at expanding HSC in vitro have proved unsuccessful and have frequently resulted in compromised rather than improved HSC grafts. (lu.se)
  • article{ff9b980d-2e89-453c-b22b-428d2e903c6f, abstract = {{Although long-term repopulating hematopoietic stem cells (HSC) can self-renew and expand extensively in vivo, most efforts at expanding HSC in vitro have proved unsuccessful and have frequently resulted in compromised rather than improved HSC grafts. (lu.se)
  • Interleukin 3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. (wikipedia.org)
  • IL-3 is a T cell-derived, pluripotent and hematopoietic factor required for survival and proliferation of hematopoietic progenitor cells. (wikipedia.org)
  • Interleukin 3 (IL-3) stimulates multipotent stem cells and differentiated hematopoietic progenitor cells, promoting their differentiation and proliferation into basophils, mast cells, type-1 and -2 dendritic cells, eosinophils and monocytes. (fujifilm.com)
  • In the presence of IL-3 Rbeta, however, IL-3 R alpha has a much higher affinity for IL-3.Emerging studies demonstrate that CD123, the IL-3 Ralpha, is highly expressed in leukemic stem cells (LSCs), while not normal hematopoietic stem cells (HSCs), and associates with treatment response, minimal residual disease (MRD) detection and prognosis. (watson-bio.cn)
  • NGF and Interleukin-3 promotes the survival, proliferation and differentiation of neurons or hematopoietic cells, respectively. (kegg.jp)
  • Eosinophils are derived from hematopoietic stem cells initially committed to the myeloid line and then to the basophil-eosinophil granulocyte lineage. (medscape.com)
  • IL-3 is produced as a monomer by activated T cells, monocytes/macrophages and stroma cells. (wikipedia.org)
  • Macrophages and dendritic cells can produce 1,25-dihydroxyvitamin D within the microenvironment. (springer.com)
  • Development of TH1 CD4 + T cells through IL-12 produced by Listeria -induced macrophages. (nature.com)
  • Indeed, several human diseases are characterized by attenuated repair responses and imbalances in the inflammatory response with increased number of infiltrating macrophages [ 3 - 5 ]. (hindawi.com)
  • Alternatively activated M2 macrophages comprise cells exposed to low concentrations of M-CSF in the presence of IL-4, IL-13, or IL-10. (hindawi.com)
  • In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)
  • IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. (biolegend.com)
  • Dendritic cells and macrophages found in large quantities at the corticomedullary junction arise from mesodermal tissues (bone marrow). (medscape.com)
  • They stimulate the proliferation of bone marrow cells in agar cultures and the formation of colonies of granulocytes and/or macrophages. (bvsalud.org)
  • Eosinophils are granulocytes (white blood cells that contain granules in their cytoplasm) derived from the same progenitor cells as monocytes-macrophages, neutrophils, and basophils. (msdmanuals.com)
  • We isolated total CD34 + cells, CD34 + ,CD33 + cells, and CD34 + ,CD33 - cells individually from normal human bone marrow cells by fluorescence-activated cell sorter (FACS), and examined the effects of granulocyte colony-stimulating factor (G-CSF), interleukin-3 (IL-3), and IL-5 on in vitro colony formation of these cells. (elsevierpure.com)
  • It has been suggested that the non-classic HLA class I molecule HLA-G plays a role in autoimmune disease by protecting tissues from damage by infiltrating cytotoxic T cells. (bmj.com)
  • Macrophage activation has been described as a dynamic process: the same cell may initially induce proinflammatory and cytotoxic reactions and later may take part in the resolution of inflammation and wound healing [ 9 ]. (hindawi.com)
  • They can be divided into 2 categories: cytotoxic, which kill infected cells directly, and helper, which make proteins called cytokines that instruct other cells to make certain molecules, move to a specific location or develop in a particular way. (nih.gov)
  • Perforin and Granzyme B) that are released by CTLs (cytotoxic T-cells) and NK (natural killer) cells. (kegg.jp)
  • These group 2 or type 2 innate lymphoid cells (ILC2 cells) represent a critical source of type 2 cytokines in vivo and serve an important role in orchestrating the type 2 response to helminths and allergens. (nature.com)
  • They have proinflammatory functions: they produce effector molecules (reactive oxygen and nitrogen intermediates) and inflammatory cytokines (IL-1 β , TNF α , and IL-6) and participate as inducer and effector cells in polarized Th1 responses. (hindawi.com)
  • This treatment induced a significant increase in the levels of proinflammatory mediators including the inducible Nitric Oxide Synthase (iNOS), interleukin 1 Beta (IL-1β) and Interleukin-12 (IL-12). (lu.se)
  • Both CD34 + cells gradually lost the CD34 antigen by day 6 of incubation with G-CSF or IL-3. (elsevierpure.com)
  • Upon their secretion by antigen-presenting cells, they exert both pro-inflammatory and anti-inflammatory receptor-mediated effects. (nature.com)
  • Controversy and debate surround the role of the non-classic human lymphocyte antigen (HLA) class I molecule HLA-G. 1, 2 Initially, the expression of HLA-G was reported to occur only in a limited subset of trophoblast cells at the fetal-maternal interface. (bmj.com)
  • The lymphocytes' selection process and developmental progression are influenced by direct contact between the TCR-CD3 complex on the thymocyte and the major histocompatibility complex (MHC)-antigen complex on thymic epithelial cells, dendritic cells, and B lymphocytes. (medscape.com)
  • IL-3 is capable of stimulating differentiation of immature myelomonocytic cells causing changes to the macrophage and granulocyte populations. (wikipedia.org)
  • IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. (wikipedia.org)
  • Activated T cells can either induce their own proliferation and differentiation (autocrine signaling), or that of other T cells (paracrine signaling) - both involve IL-2 binding to the IL-2 receptor on T cells (upregulated upon cell activation, under the induction of macrophage-secreted IL-1). (wikipedia.org)
  • It was concluded that G-CSF supported the neutrophil differentiation of committed colony-forming cells, IL-3 supported that of both committed and multipotent colony-forming cells. (elsevierpure.com)
  • Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. (bdbiosciences.com)
  • function of proliferative responses provoked in the induction cell products favor immediate type hypersensitivity reactions, phase of contact allergy, the murine local lymph node assay serving as growth and differentiation factors for mast cells and (LLNA), has been accepted as a stand-alone method for skin eosinophils, the cellular effectors of the clinical manifestations of sensitization testing (NIH, 1999). (cdc.gov)
  • Eosinophilic differentiation occurs in the bone marrow from myeloid progenitors through the actions of GM-CSF, IL-3, and IL-5. (medscape.com)
  • Here, in the presence of KL+FL+megakaryocyte growth and development factor (MGDF), which recruits virtually all Lin(-)Sca-1(+)kit(+) bone marrow cells into proliferation and promotes their self-renewal under serum-free conditions, IL-3 and IL-11 revealed an indistinguishable ability to further enhance proliferation. (lu.se)
  • Post-chemotherapy, IL-3 application reduces chemotherapy delays and promotes regeneration of granulocytes and platelets. (wikipedia.org)
  • Interleukin 25 promotes the initiation of proallergic type 2 responses. (nature.com)
  • Numerous reports suggest that interleukin-6 (IL-6) promotes survival and proliferation of tumor cells through the phosphorylation of a cell-signaling protein, signal-transducer-and-activator-of-transcription-3 (STAT3). (greenmedinfo.com)
  • In studies on mouse mammary carcinoma, the IL-33/ST2 pathway promotes BC progression and metastasis through increased intratumoral accumulation of immunosuppressive cells and by diminishing innate anti-tumor immunity ( 11 ). (frontiersin.org)
  • IL4 promotes phagocytosis of murine leukemia cells counteracted by CD47 upregulation. (lu.se)
  • [ 8 ] Another experimental model of protein contact dermatitis induced the generation of T cells, the infiltration of eosinophils, and the production of IL-4 and IL-5. (medscape.com)
  • To study the role of peritoneal mast cells during in vivo infection with S. aureus we used newly developed Mcpt5-Cre(+) x R-DTA mice in which mast cell deficiency is independent of c-Kit. (uni-koeln.de)
  • This is in contrast to previous studies in which an impact of mast cells on bacterial infection has been proposed based on the use of mice whose mast cell deficiency is a consequence of defective c-Kit signalling. (uni-koeln.de)
  • Seventy percent of thymomas are associated with paraneoplastic syndromes such as myasthenia gravis (MG), red cell aplasia, pemphigus, and immunoglobulin (Ig) deficiency. (medscape.com)
  • Neither the title of the paper, nor the abstract mentioned that in the experiment, five out of 25 rats receiving the transplant died with "teratoma-like tumors" in their brains, a well-known hazard of ES cells. (i-sis.org.uk)
  • IL-3 is mainly produced by activated T cells with the goal of initiating proliferation of various other immune cell types. (wikipedia.org)
  • The initiation of type 2 immune responses by the epithelial cell-derived cytokines IL-25, IL-33 and TSLP has been an area of extensive research in the past decade. (nature.com)
  • Interactions between ILC2 cells and the adaptive immune system, as well as examination of potential roles for ILC2 cells in the maintenance of homeostasis, promise to be particularly fruitful areas of future research. (nature.com)
  • Saenz, S.A., Taylor, B.C. & Artis, D. Welcome to the neighborhood: epithelial cell-derived cytokines license innate and adaptive immune responses at mucosal sites. (nature.com)
  • Stereotactic Body Radiation and Interleukin-12 Combination Therapy Eradicates Pancreatic Tumors by Repolarizing the Immune Microenvironment. (rochester.edu)
  • The alterations of the immune system are characterized by a high number of activated T and B cells, elevated immunoglobulin secretion, anti-colon antibodies, and aberrant expression of class II major histocompatibility complex molecules. (nih.gov)
  • 10 Coupled with the reported tissue distribution, these inhibitory effects upon immune cells have led to the suggestion that HLA-G has a role in maintaining maternal-fetal tolerance, 11 reducing transplant rejection, 12 and in allowing the progression of tumours. (bmj.com)
  • Immune cells in the mouse spleen, a site of Th17 development. (nih.gov)
  • The immune system employs many different types of cells to regulate disease. (nih.gov)
  • T cells play a major role in the immune system. (nih.gov)
  • There's a mechanistic role in T-cell trafficking and a significant effect as it relates to the immune function. (medscape.com)
  • Вроджений імунітет The immune system distinguishes self from nonself and eliminates potentially harmful nonself molecules and cells from the body. (msdmanuals.com)
  • Successful completion of these projects will identify new therapeutic targets in acute myleoid leukemia (AML) and expand our knowledge regarding how cancer cells evade the immune system, findings that may translate into new therapeutic opportunities. (lu.se)
  • Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist. (bdbiosciences.com)
  • Seder, R. A., Gazzinelli, R., Sher, A. & Paul, W. E. Interleukin 12 acts directly on CD4+ T cells to enhance priming for interferon gamma production and diminishes interleukin 4 inhibition of such priming. (nature.com)
  • Bacitracin prevents the transfer of mucopeptides into the growing cell wall, which causes inhibition of bacterial cell wall synthesis. (medscape.com)
  • These results suggest that genetic down-regulation or pharmacological inhibition of galectin-3 might constitute a novel therapeutic target in PD and other synucleinopathies. (lu.se)
  • With a basis in stem cell biology, genetics, immunology and cancer, the major goal of our research is to develop new immunotherapies that target leukemia and other malignancies. (lu.se)
  • Horvitz and his colleague Junying Yuan [2] found in 1993 that the protein encoded by the ced-3 gene was a cysteine protease with similar properties to the mammalian interleukin-1-beta converting enzyme (ICE) (now known as caspase 1) which at the time was the only known caspase. (wikidoc.org)
  • these proteins are toxic to several parasites and to mammalian cells. (msdmanuals.com)
  • Parkinson's patients carrying mutations in PINK1 and Parkin genes have increased levels of circulating interleukin 6 and mitochondrial DNA. (neurosciencenews.com)
  • After co-culture of peritoneal mast cells with live S. aureus we found by gene array analysis that they up-regulate a number of genes. (uni-koeln.de)
  • But, although the mutation frequency of genes was much lower in ES cells, mutant ES cells accumulated with time in culture. (i-sis.org.uk)
  • The OSMR and IL31RA genes provide instructions for making parts (subunits) of the interleukin-31 (IL-31) receptor. (medlineplus.gov)
  • Core Circadian Clock Genes Regulate Leukemia Stem Cells in AML. (lu.se)
  • IL-6 synonyms: plasmacytoma growth factor (PCT-GF),interferon-a-2 (IFN-a2), monocyte derived human B cellgrowth factor, B cell stimulating factor (BSF-2),hepatocyte stimulating factor (HSF), and interleukinhybridoma/plasmacytoma-1 (IL-HP1). (abcam.com)
  • Furthermore, high-resolution cell division tracking demonstrated that all HSC underwent a minimum of 5 cell divisions, suggesting that long-term repopulating HSC are not compromised by IL-3 stimulation after multiple cell divisions. (lu.se)
  • IL-3 is produced by T cells only after stimulation with antigens or other specific impulses. (wikipedia.org)
  • Immunologic and confocal microscopic evidence also suggested a transient but significant stimulation of tyrosine phosphorylation of Cav-1 in β-cells briefly (for 15 min) exposed to IL-1β that was markedly attenuated by three structurally distinct inhibitors of protein tyrosine phosphorylation. (diabetesjournals.org)
  • To test whether extracts also affected normal cells, similar experiments were also performed using isolated peripheral blood lymphocytes from healthy volunteers, with and without stimulation by the mitogen phytohemagglutinin (PHA). (nih.gov)
  • The signal transmission is ensured by high affinity between cell surface interleukin-3 receptor and IL-3. (wikipedia.org)
  • Valent P, Besemer J, Muhm M, Majdic O, Lechner K, Bettelheim P. Interleukin 3 activates human blood basophils via high-affinity binding sites. (bdbiosciences.com)
  • Additionally, tacrolimus may inhibit release of preformed mediators from skin mast cells and basophils and may down-regulate expression of high-affinity IgE receptor (FCeRI) on Langerhans cells. (medscape.com)
  • A novel human cell line, KMT-2, from umbilical cord blood cells was established based on the selection of cultures in the presence of recombinant human interleukin-3 (IL-3) and the sorting of cells with anti-My 10 antibody. (ashpublications.org)
  • Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
  • The MP2-43D11 antibody can not neutralize the bioactivity of natural or recombinant IL-3. (biolegend.com)
  • To obtain a linear standard curve, serial dilutions of IL-3 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. (biolegend.com)
  • Eosinophil (Eo) colonies were only formed by CD34 + ,CD33 - cells in response to IL-3, but scarcely formed by CD34 + cells in the presence of IL-5. (elsevierpure.com)
  • These cytokines are also the primary signals that inhibit eosinophil programmed cell death. (medscape.com)
  • Thus, eosinophilia can be triggered via these 3 eosinophilopoietic cytokines by increased eosinophil production, by eosinophil longevity, or by a combination of these. (medscape.com)
  • Although GM-CSF and IL-3 also increase the production of other myeloid cells, IL-5 increases eosinophil production exclusively. (msdmanuals.com)
  • We performed the two-step cultures consisting of the primary liquid culture for 6 days and the secondary methylcellulose culture, and serially examined changes in phenotypes of the cells cultured in the primary culture. (elsevierpure.com)
  • A phorbol-12- myristate-13-acetate (16561298) (PMA) dose of 10 nanograms per milliliter, a phenol (108952) dose of 1mg/ml, and a sodium-arsenite (7784465) concentration of 4 millimolar were added to cell cultures. (cdc.gov)
  • Different volumes of 70% ethanol and water extracts of I'm-Yunity were incubated with cultures of human promyelocytic leukemic HL-60 cells, and compared to nontreated control cells. (nih.gov)
  • Mice that received Th17 cells from IL-23 cultures showed more severe symptoms of EAE. (nih.gov)
  • IL-3 signaling is able to give rise to widest array of cell linages which is why it has been independently named "multi-CSF" in some older literature. (wikipedia.org)
  • We recently reported that the activation of H-Ras represents one of the signaling steps underlying the interleukin-1β (IL-1β)−mediated metabolic dysfunction of the islet β-cell. (diabetesjournals.org)
  • Overexpression of an inactive mutant of Cav-1 lacking the tyrosine phosphorylation site (Y14F) or an siRNA-mediated Cav-1 knock down also resulted in marked attenuation of IL-1β-induced iNOS gene expression and NO release from these cells, thus further implicating Cav-1 in this signaling cascade. (diabetesjournals.org)
  • Here we provide the first evidence to suggest that tyrosine phosphorylation of Cav-1 and subsequent interaction among members of the Ras signaling pathway within the membrane lipid microdomains represent early signaling mechanisms of IL-1β in β-cells. (diabetesjournals.org)
  • Interleukin 3 (IL-3) is a protein that in humans is encoded by the IL3 gene localized on chromosome 5q31.1. (wikipedia.org)
  • The human IL-3 gene encodes a protein 152 amino acids long, and the naturally occurring IL-3 is glycosylated. (wikipedia.org)
  • With hematopoiesis of IL-3, this protein is anticipated to be an effective treatment, in combination with chemotherapy, for myelodysplastic syndrome and aplastic anemia. (fujifilm.com)
  • [ 1 , 2 ] The specific criteria for protein contact dermatitis are (1) a chronic dermatitis caused by contact with proteinaceous material, (2) an acute urticarial or vesicular eruption occurring minutes after contact with the causative protein, (3) immediate prick- or scratch-test results that are usually positive, and (4) patch-test results that are often negative. (medscape.com)
  • A mouse model of protein contact dermatitis induced by natural rubber latex revealed an increase in CD4 + CD3 + T cells and mast cells and a T H 2-type response with a strong IgE-mediated response. (medscape.com)
  • SP600125 inhibited interleukin-1β-induced JNK activity and activator protein-1 activation, but it did not affect the activation of extracellular-regulated kinase, p38 mitogen-activated protein kinase, and nuclear factor-κB. (aspetjournals.org)
  • CASP3 , CASP6 , CASP7 ) in turn cleave other protein substrates within the cell resulting in the apoptotic process. (wikidoc.org)
  • Galectin-3 is a member of a carbohydrate-binding protein family involved in cell activation and inflammation. (lu.se)
  • The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein (S) plays critical roles in host cell entry. (cdc.gov)
  • Interleukin 3 was originally discovered in mice and later isolated from humans. (wikipedia.org)
  • Mice deficient for interleukin-2 develop normally during the first 3-4 weeks of age. (nih.gov)
  • The CD123 antibody, clone 9F5, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with IL-3Rα-transfected COS cells. (bdbiosciences.com)
  • They transferred the cells into mice engineered to lack mature T cells of their own. (nih.gov)
  • Staphylococcus aureus was injected intraperitoneally into mast-cell-deficient Mcpt5-Cre(+) x R-DTA mice using littermate mast-cell-sufficient mice as controls. (uni-koeln.de)
  • Here, we investigated bleomycin-induced lung injury responses in young and aged mice at single-cell resolution to gain insights into the cellular and molecular contributions of aging to fibrosis. (researchgate.net)
  • We confirmed these findings using primary microglial cells obtained from wild-type and galectin-3 null mutant mice. (lu.se)
  • Finally, we performed injections of α-synuclein in the olfactory bulb of wild type mice and observed that some of the α-synuclein was taken up by activated microglia that were immunopositive for galectin-3. (lu.se)
  • Leukemia , 2018 Mar;32(3):588-596. (lu.se)
  • IL1RAP antibodies block IL-1-induced expansion of candidate CML stem cells and mediate cell killing in xenograft models. (lu.se)
  • Objectives Granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA) are autoimmune vasculitides associated with antineutrophil cytoplasm antibodies that target proteinase 3 (PR3) or myeloperoxidase (MPO) found within neutrophils and monocytes. (bmj.com)
  • Granulomas are exclusively found in GPA and form around multinucleated giant cells (MGCs), at sites of microabscesses, containing apoptotic and necrotic neutrophils. (bmj.com)
  • The term lymphoepithelioma has been used in cases in which the thymoma contains a large number of lymphoid cells. (medscape.com)
  • From the corticomedullary junction, the cells enter the medulla or circulate in the periphery to other lymphoid structures (ie, lymph nodes). (medscape.com)
  • IL-3 is also involved in the reconstruction of platelets via the development of megakaryocytes. (wikipedia.org)
  • The interleukin-1 (IL-1) family is a growing group of cytokines, consisting of at least 11 members, and the balance between pro- and anti-inflammatory cytokines is crucial in the pathogenesis of many human diseases ( 3 ). (frontiersin.org)
  • In the present report, we demonstrate that most HNSCC cell lines had constitutively active STAT3 and that curcumin (diferuloylmethane), a pharmacologically safe agent in humans, inhibited STAT3 phosphorylation in a dose- and time-dependent manner. (greenmedinfo.com)
  • Interleukin-33 (IL-33) is an important member of the IL-1 family, and in humans is expressed predominantly in skin, lung, adipocytes, and synovial fibroblasts ( 4 ). (frontiersin.org)
  • Only two of these genotypes, A and B (Nash Groups 1/2 and 3 ( 150 , 151 ) are found in humans. (antimicrobe.org)
  • If you see this knockout in humans, they really don't respond well to any therapy other than stem cell transplant. (medscape.com)
  • In the early 1960s Ginsberg and Sachs discovered that IL-3 is a potent mast cell growth factor produced from activated T cells. (wikipedia.org)
  • A better understanding of the complex signals between these cells will help researchers design better ways to prevent and treat autoimmune diseases. (nih.gov)
  • TNF family of ligands activates anti-apoptotic or cell-survival signals as well as apoptotic signals. (kegg.jp)
  • This attachment triggers a series of chemical signals inside the cell that directs certain cell functions. (medlineplus.gov)
  • The cleavage and inactivation of ICAD/DFF45 by a caspase allows CAD to enter the nucleus and fragment the DNA, causing the characteristic 'DNA ladder' seen in apoptotic cells. (wikidoc.org)
  • Since patients with GPA have augmented neutrophil PR3 expression, and PR3-expressing apoptotic cells frustrate macrophage phagocytosis and cellular clearance, we investigated the role of PR3 in stimulating giant cell and granuloma formation. (bmj.com)
  • Peritoneal mast cells have been implicated in the host response towards various bacterial insults and to provide mechanistic insight into the role of mast cells in intraperitoneal bacterial infection we here studied the global effects of S. aureus on mast cell gene expression. (uni-koeln.de)
  • Cephalexin is a first-generation cephalosporin that arrests bacterial growth by inhibiting bacterial cell wall synthesis. (medscape.com)
  • IL-3 Ralpha binds IL-3 with relatively low affinity. (watson-bio.cn)
  • We conclude that, despite peritoneal mast cells being activated by S. aureus in vitro, they do not influence the in vivo manifestations of intraperitoneal S. aureus infection. (uni-koeln.de)
  • Results In vitro, PR3 promoted monocyte-derived MGC formation using cells from patients with GPA but not from patients with MPA, and this was dependent on soluble interleukin 6 (IL-6), as well as monocyte MAC-1 and protease-activated receptor-2, found to be overexpressed in the cells of patients with GPA. (bmj.com)
  • Background: Vestigial like family member 3 (VGLL3) is associated with the prognosis of epithelial ovarian cancer and soft tissue sarcoma, but its role in gastric cancer (GC) is unclear. (researchgate.net)
  • The epithelial cells and these other stromal tissues of the thymus influence the selection and maturation of the T lymphocytes. (medscape.com)
  • Disruption of lipid rafts (e.g., with cyclodextrin) markedly reduced IL-1β−induced gene expression of inducible NO synthase (iNOS) and NO release from β-cells. (diabetesjournals.org)
  • Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin. (bdbiosciences.com)
  • Arrayed molecular barcoding identifies TNFSF13 as a positive regulator of acute myeloid leukemia-initiating cells. (lu.se)
  • The cells could be made to differentiate into bone forming cells, cartilage forming cells, fat cells, skeletal muscle cells and endothelial cells. (i-sis.org.uk)
  • Targeting constitutive and interleukin-6-inducible signal transducers and activators of transcription 3 pathway in head and neck squamous cell carcinoma cells by curcumin (diferuloylmethane). (greenmedinfo.com)
  • Constitutive activation of STAT3 in head and neck squamous cell carcinoma (HNSCC) and its role in proliferation of this tumor has been demonstrated. (greenmedinfo.com)
  • TSLP acts on infiltrating effector T cells to drive allergic skin inflammation. (nature.com)
  • These two pathways converge at the level of effector caspases, such as caspase-3 and caspase-7. (kegg.jp)
  • A subset of such mutations enable escape from neutralizing antibodies or are thought to enhance transmission through mechanisms such as increased affinity for the cell entry receptor, angiotensin-converting enzyme 2 (ACE2). (cdc.gov)
  • Interleukin-12 (IL-12) and interleukin-23 (IL-23), which belong to the IL-12 family of cytokines, have a key role in intestinal homeostasis and inflammation and are implicated in the pathogenesis of inflammatory bowel disease. (nature.com)
  • This agent decreases inflammation by stabilizing lysosomal membranes, inhibiting polymorphonuclear leukocyte and mast cell degranulation. (medscape.com)
  • Reduces itching and inflammation by suppressing release of cytokines from T cells. (medscape.com)
  • Previous studies have shown that extracellular α-synuclein aggregates can activate microglial cells, induce inflammation and contribute to the neurodegenerative process in PD. (lu.se)
  • Conclusions: We show that α-synuclein aggregates induce microglial activation and demonstrate for the first time that galectin-3 plays a significant role in microglia activation induced by α-synuclein. (lu.se)
  • The human IL-3 gene is located on chromosome 5, only 9 kilobases from the GM-CSF gene, and its function is quite similar to GM-CSF. (wikipedia.org)
  • Human IL-3 was first cloned in 1986 and since then clinical trials are ongoing. (wikipedia.org)
  • Here, we show that Mcl-1 down-regulation by the cyclin-dependent kinase (CDK) inhibitor roscovitine or Mcl-1-shRNA dramatically increases ABT-737 lethality in human leukemia cells. (aacrjournals.org)
  • SARS-CoV-2, the virus responsible for COVID-19, enters human cells by attaching to ACE2 and utilizing TMPRSS2. (neurosciencenews.com)
  • In contrast, Th17 cells developed with IL-23 showed higher levels of IL-18R1 and T-bet-2 molecules known to be involved in the development of experimental autoimmune encephalomyelitis (EAE), a mouse disease that resembles human multiple sclerosis. (nih.gov)
  • Britain s House of Lords final approval of therapeutic human cloning and embryonic stem cells research has intensified the battle for ascendancy between adult and embryonic stem cells. (i-sis.org.uk)
  • In the present work, we have isolated and characterized the extracellular vesicles from human AD-MSC to investigate their role in the chondroprotective actions of these cells. (karger.com)
  • In this study production levels of interleukins (IL)-12 and IL-13 were measured by commercial ELISA in culture supernatants of mitogen-stimulated peripheral blood mononuclear cells from 30 non-splenectomized beta-thalassaemia cases with iron overload and 20 age- and sex-matched healthy individuals. (who.int)
  • Recent studies demonstrated that high serum levels of soluble ST2 (sST2) are a worse prognostic factor in hepatocellular carcinoma ( 9 ), and that serum IL-33 is a diagnostic and prognostic marker in non-small cell lung cancer, independent of the therapeutic intervention ( 10 ). (frontiersin.org)
  • Vitamin D inhibits proliferation and profibrotic marker expression in hepatic stellate cells and decreases thioacetamide-induced liver fibrosis in rats. (springer.com)
  • Curcumin inhibits head and neck cancer cell proliferation. (greenmedinfo.com)
  • This agent selectively inhibits production and release of inflammatory cytokines from activated T cells by binding to cytosolic immunophilin receptor macrophilin-12. (medscape.com)
  • However, only IL-3 treatment in bone marrow failure disorders such as myelodysplastic syndrome (MDS) and aplastic anemia (AA) was disappointing. (wikipedia.org)
  • These researchers pointed out that the spontaneous fusion rate (without interleukin-3) was extremely low, between 2-11 per million bone marrow cells, and is unlikely to account for all the findings with adult stem cells. (i-sis.org.uk)
  • In the normal thymus, bone marrow-derived precursor cells destined to become thymocytes (or T lymphocytes) enter the thymus at the corticomedullary junction and differentiate as they pass through the thymus. (medscape.com)