Cells identify flow cytometry. Medical search. Frequent questions

Normal maturing PDC populations we found to make up less than 0.5% of adult bone marrow and are therefore difficult to detect by flow cytometry. (news-medical.net)
Single-cell phospho-specific flow cytometry for detection of basal as well as activation-induced phosphorylation of signaling proteins in distinct cell populations can be used to identify aberrant signaling pathways. (biomedcentral.com)
The overall goal of this study is to compare two different methods of human monocyte isolation for dendritic cell generation in vitro and to characterize the CT11c, CT14 cell surface expression of the resulting monocyte derived populations by imaging flow cytometry. (jove.com)
The use of different fluorochromes helps researchers distinguish between cell populations or different cellular activities. (bdbiosciences.com)
Transcriptome comparisons of SOX9+ cells with GLT1+ cells showed that the two populations of cells exhibit largely overlapping gene expression. (jneurosci.org)
Different impacts of exercise were seen for lymphocytes, B cells, especially the IgD(+) subpopulation of B cells and the concentrations of IP-10, RANTES and DHEA-S. We conclude that prolonged bed rest significantly impacts immune cell populations and cytokine concentrations. (galileo-training.com)
In Bioinformatics, Alex has developed algorithms for registering and classifying cell populations in Flow Cytometry data to understand the immune system. (purdue.edu)
In three cases which initially were taken for pleomorphic adenomas by routine histological examination, aneuploid cell populations exposed by DNA flow cytometric analysis gave rise to a closer inspection of the suspect lesions. (researchgate.net)
By utilising flow cytometry, researchers can accurately identify and measure specific cell populations within complex mixtures, as well as assess cell cycle progression, proliferation status, and expression of fluorescent markers. (embl.org)
The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations. (biolegend.com)
As the field evolves, the traditional method of manual gating by sub-setting single cell data into populations and encircling data points in hand-drawn polygons in two-dimensional space has proven laborious, subjective, and difficult to standardise. (biorxiv.org)

The researcher can then take the cells of interest and grow them in culture or study them in other assays. (bdbiosciences.com)
CVA and CVA for CELL-DYN provide assays for Abbott, Beckman Coulter, HemoCue, Horiba Medical and Mindray analyzers to assess calibration verification. (streck.com)
Our course aims to provide a comprehensive understanding of the underlying principles of flow cytometry, while also emphasising practical considerations related to the most frequently used assays. (embl.org)
The efforts to find clinical utility of assays to detect these self-reactive T cells and their role in disease pathogenesis using both patient samples, and human T-cell receptor based transgenic animal models will be the next focus of the group. (reachmd.com)
The in vitro effects of the drugs on CRC cells were tested using CCK8 and flow cytometry assays. (lu.se)
In addition, the sensitivity of detecting cell-free DNA (cfDNA) in next-generation sequencing (NGS) assays requires substantial improvement. (kaloramainformation.com)
The CCK-8, EdU, scratch wound healing, and transwell invasion assays revealed that METTL16 significantly suppressed the proliferating, migrating, and invading abilities of OC cells. (bvsalud.org)

However, there are conflicting results whether exposure to anti-IgM in vitro promotes or suppresses apoptosis in CLL cells [ 6 ] and other signals provided by the tumor microenvironment likely determines the outcome [ 7 ]. (biomedcentral.com)
Interferon (IFN)-γ synthesis by T cells after an in vitro. (cdc.gov)
Similar cell cycle compartments are also recognized by multiparameter analysis that includes measurement of expression of cyclin D1, cyclin E, cyclin A and cyclin B1, each in relation to DNA content Concurrent measurement of DNA content and of incorporation of DNA precursor 5-bromo-2'-deoxyuridine (BrdU) by flow cytometry is an especially useful assay, that has been widely used in analysis of the cell cycle in vitro and in vivo. (wikipedia.org)
This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). (jove.com)
Further, inhibiting I-kappa-B, which activates NF-kappa-B signaling, was shown to induce cell kill in vitro in PMBCL cell-lines. (medscape.com)
Using in vitro cell cultures, Bajpai developed a novel method to achieve this goal that detects and quantifies the melanin-producing activity of melanocytes. (ou.edu)
Using a powerful in vitro platform, we have determined that the severity of clinical manifestations associated with mutations in the RAG, DCLRE1C, and LIG4 genes correlates with the residual activity of the mutant protein and with different degrees of perturbation of T- and B-cell repertoire diversity and composition. (nih.gov)
TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro , hemorrhagic necrosis of tumors in vivo , increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. (biolegend.com)
The aim of the present study was to identify whether P. gingivalis heat‑shock protein 60 (HSP60) induced the dysfunction of human umbilical vein endothelial cells (HUVECs) in vitro. (spandidos-publications.com)
This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)

Multicolor flow cytometry provides rapid, multiparametric analysis of various cellular subpopulations of interest. (bdbiosciences.com)
From flow cytometers and sorters for simple to complex research applications to an extensive selection of reagents and a comprehensive collection of educational resources , tools such as the Interactive Cell Map and protocols , we support you in efficiently navigating your multicolor flow cytometry workflow journey. (bdbiosciences.com)
The study utilized multicolor flow-cytometry based immunophenotyping of T cells from 72 patients with AAV. (reachmd.com)

Flow cytometric analysis of T-cell responses to smallpox antigens after recent smallpox vaccination and in long-term vaccinated or not vaccinated persons. (cdc.gov)
Methods: Flow cytometric analysis of DNA pliody and S-Phase Fraction (SPF) was done in 50 fresh tumor tissue sections of SGTs which diagnosed as 15 benign and 35 malignant tumors. (researchgate.net)
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis . (biolegend.com)
Flow cytometric analysis of archived lesions of proliferative verrucous leukoplakia has shown evidence of aneuploid cell lines, with DNA indexes remaining constant throughout the course of disease in most cases. (medscape.com)

assessed by immunohistochemistry or flow cytometry, by using a handful of phenotypic markers. (lu.se)
In certain circumstances, when a lymph node is not easily accessible for excisional or incisional biopsy, a combination of core biopsy and FNA biopsies in conjunction with appropriate ancillary techniques for the differential diagnosis (immunohistochemistry, flow cytometry, PCR for IGHV and TCR gene rearrangements, karyotype, and fluorescence in situ hybridization [FISH] for major translocations) may be sufficient for diagnosis. (medscape.com)

To achieve a proper separation of the peripheral blood mononuclear cells, load the blood onto the density gradient solution quickly, but carefully and without mixing the layers. (jove.com)
To identify and characterize immune response changes, they analyzed whole blood and peripheral blood mononuclear cells using flow cytometry and RNA sequencing. (umn.edu)
Lung histopathology reveals interstitial infiltration with mononuclear cells, well-defined noncaseating granulomas (sometimes with multinucleated giant cells and calcific inclusions), and varying degrees of pulmonary fibrosis (Meyer 1994). (cdc.gov)

Multiple sclerosis (MS) is a neuroinflammatory disease with an autoimmune component that is characterised by activation of self-reactive lymphocytes, which enter the central nervous system (CNS) and cause destruction of myelin producing cells and neurons leading to the formation of inflammatory lesions. (nature.com)
In CLL, the bone marrow makes abnormal lymphocytes (a type of white blood cell). (medlineplus.gov)
In addition to lymphocytes, dendritic cells can also produce LT α [ 15 ]. (hindawi.com)
Ly6C low MC manifested activated T-cell signaling pathways and potentially can adapt the function of lymphocytes. (frontiersin.org)
Although the cells of origin are lymphocytes, PCNSL should be considered a brain tumor, because the therapeutic challenges resemble those of other brain tumors. (medscape.com)
An accumulation of lymphocytes, especially CD4 + T cells, in TPE has been well documented ( 2 ). (atsjournals.org)
Flow cytometric estimation of the apoptotic marker CD95 in peripheral neutrophils, lymphocytes and monocytes was done for 18 infants with non-oedematous protein energy malnutrition (PEM) and 12 oedematous ones, on hospital admission and after supervised nutritional rehabilitation, and compared with 12 matched controls. (who.int)
and the percentage of lymphocytes that are CD4+ T-cells. (cdc.gov)
The process of measuring the percentage of CD4+ T-lymphocytes in the whole blood sample is referred to as ``immunophenotyping by flow cytometry'' (9-14). (cdc.gov)
A significant number of non-neoplastic T cells (resting CD4 + T cells and activated small- or medium-sized CD8 + lymphocytes) may coexist with a minor neoplastic T-cell population. (medscape.com)
The bronchoalveolar lavage (BAL) fluid from a patient with CBD typically reveals evidence of lung inflammation, indicated by an elevated white blood cell count with an increased number of lymphocytes. (cdc.gov)

A research team from the University Hospitals of Cleveland Medical Center in western Pennsylvania has discovered the existence of myeloid neoplasms (MNs) that can undergo plasmacytoid dendritic cell (PDC) differentiation. (news-medical.net)
Both categories of cells resulting from atypical PDC differentiation are thought to represent two ends of a spectrum of maturation. (news-medical.net)
Leukemic myoblast differentiation into PDCs occurs as a result of sharing a common hematopoietic precursor to dendritic cells, monocytes, and myeloid cells. (news-medical.net)
BCR is important for the proliferation, differentiation and apoptosis of B cells [ 3 ]. (biomedcentral.com)
The main advantage of these protocols is that they facilitate the isolation of human monocytes and their differentiation into viable and functional monocyte derived cells. (jove.com)
Ly6C high MC presented downregulated co-stimulatory receptors (CD2, GITR, and TIM1) which direct immune cell proliferation, and upregulated co-stimulatory ligands (LIGHT and SEMA4A) which trigger antigen priming and differentiation. (frontiersin.org)
A ) Morphology of Nrl -GFP wild-type (WT) and rd16 retinal organoids differentiated from mouse-induced pluripotent stem cells (iPSC) at various differentiation time points. (elifesciences.org)
generation and targeted differentiation of induced pluripotent stem cells) to identify novel gene defects. (nih.gov)
By modeling human T-cell differentiation in an artificial thymic organoid system, our lab is interested in defining the stages at which T-cell development is blocked in patients with known and novel forms of severe combined immune deficiency. (nih.gov)
S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
However, differentiation and immune regulation of Th22 cells in tuberculous pleural effusion (TPE) remain unknown. (atsjournals.org)
The impacts of proinflammatory cytokines and antigen presentation by pleural mesothelial cells (PMCs) on Th22-cell differentiation were explored. (atsjournals.org)
IL-1β, IL-6, and/or tumor necrosis factor-α promoted Th22-cell differentiation from CD4 + T cells. (atsjournals.org)
Moreover, PMCs were able to stimulate CD4 + T-cell proliferation and Th22-cell differentiation by presenting tuberculosis-specific antigen. (atsjournals.org)
In particular, PMCs were able to function as antigen-presenting cells to stimulate CD4 + T-cell proliferation and Th22-cell differentiation. (atsjournals.org)
Pleural mesothelial cells stimulate CD4 + T-cell proliferation and Th22 cell differentiation in response to tuberculosis antigen. (atsjournals.org)
Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
In this study, highly purified, flow-cytometry sorted, classified in relation to normal B-cell differentiation [1]. (lu.se)
Within the B-cell and T-cell categories, two subdivisions are recognized: precursor neoplasms, which correspond to the earliest stages of differentiation, and more mature differentiated neoplasms. (medscape.com)

The tumor cells resemble immunoblasts or centroblasts, and, in many cases, sclerosis and fibrosis can be identified. (medscape.com)
The results demonstrated that exosomes derived from both tumor cells and normal cells exhibit special, unique Raman phenotypes. (mdpi.com)
He noted that recurrence can sometimes occur in the absence of B-cell recovery, in the form of tumor cells that do not express CD19 and can therefore evade functional CAR-T cells. (aacr.org)
This may be due to late diagnosis of these tumors in our patients as most cases diagnosed at advanced stage and the sections were taken from deep tumor tissue to increase the number of tumor cells in our samples. (researchgate.net)
Advances in liquid biopsy techniques will also contribute to growth by enabling tumor cells to be extracted more easily. (kaloramainformation.com)
Advances in liquid biopsy technologies will foster growth by expanding the number of tumor cells that can be readily extracted for analysis. (kaloramainformation.com)
GEP analyses were performed on highly purified, flow-cytometry sorted tumor-cells from eight subgroups of BCLs. (lu.se)
This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). (lu.se)

To infer functional relationships between SLCs and metabolites, we developed a strategy to identify SLCs able to sustain cell viability and proliferation under growth-limiting concentrations of essential nutrients. (life-science-alliance.org)
One-by-one depletion of 13 amino acids required for cell proliferation enabled gain-of-function genetic screens using a SLC-focused CRISPR/Cas9-based transcriptional activation approach to uncover transporters relieving cells from growth-limiting metabolic bottlenecks. (life-science-alliance.org)
B ) Morphology of induced pluripotent stem cells (iPSC) colonies, ( C ) proliferation rate of iPSCs, and ( D ) photoreceptor primary cilium of organoids were compared between WT and rd16 . (elifesciences.org)
The proliferation of names reflects initial uncertainty about the cell of origin. (medscape.com)
Acute Myeloid Leukemia (AML) In acute myeloid leukemia (AML), malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived myeloid progenitor cell results in high circulating numbers. (msdmanuals.com)
Cells from bronchoalveolar lavage should be tested with the beryllium lymphocyte proliferation test (BeLPT) as previously described in the Clinical Assessment section. (cdc.gov)

When the high similarity between the orthopoxviruses is considered, this rapid assay that uses vaccinia antigens could identify recently exposures. (cdc.gov)
Autoreactive T cell responses were screened using a multicolor fluorospot assay, while the repertoire of such cells was investigated using TCR sequencing of single-sorted T cells. (reachmd.com)
In conclusion, activation-induced CD137 expression is a fast assay allowing for detection and functional analysis of the total alloreactive T cell compartment at the single-cell level by multi-parameter flow cytometry. (eur.nl)
The inhibitory effects of METTL16 on the in vivo tumor growth of EOC cells were measured by subcutaneous tumor formation assay in mice. (bvsalud.org)
Furthermore, the RIP, RNA stability assay, western blotting, and cytoimmunofluorescence staining showed that METTL16 hindered the growth of EOC cells through promoting the degradation of MALAT1 by binding that, in turn, upregulates ß-catenin protein and promotes nuclear transport of ß-catenin protein in EOC cells. (bvsalud.org)
Using a sphere-forming assay, identified. (lu.se)

The major breakthrough is the identification of a biomarker of HIV persistent cells in vivo. (europa.eu)
AOSLO flow cytometry provides in vivo ratios of WBCs and RBCs, an important component of the CBC - which currently requires a blood draw and ex vivo analysis. (arvojournals.org)
Finally, we show that antibodies directed against C3AR efficiently elicit NK cell-mediated killing of primary AML cells ex vivo, highlighting C3AR as a candidate therapeutic target in NPM1-mutated AML. (lu.se)

The migration of inflammatory leukocytes, including dendritic cells, neutrophils, and macrophages, to the peritoneal mesentery has been shown to be promoted in LAT −/− (lack mature T cells) mice but inhibited in μ MT (lack mature B cells) mice during the pristane-induced immune response [ 10 ]. (hindawi.com)
Composition of immune cells was analyzed by flow cytometry. (galileo-training.com)
Monocytes (MC) are bone marrow (BM) derived mononuclear phagocytes that play an important role in innate immune response and are the major immune cell population in chronic tissue inflammatory ( 1 , 2 ). (frontiersin.org)
Blood and saliva samples will be analysed for markers of inflammation and immune function, using cytometric bead array and flow cytometry techniques. (bmj.com)
Using this approach, the laboratory has identified several new gene defects, including DOCK2 deficiency, TTC7A deficiency (responsible for combined immune deficiency and intestinal atresias), HOIP deficiency (a cause of immune deficiency and autoinflammatory disease), EXTL3 deficiency (associating severe T-cell immunodeficiency, skeletal dysplasia, and neurodevelopmental delay), PAX1 deficiency and SASH3 deficiency. (nih.gov)
T-cell receptor (TCR) repertoire of PR3-reactive T cells showed shared (public) TCRs between patients, implicating a common "clonal" immune response . (reachmd.com)
In the longer perspective, future therapies can be aimed at specific depletion of such T cells or re-educating the immune system by designing autoantigen-based vaccines. (reachmd.com)
The main challenge involves determining the threshold for circulating tumor DNA (ctDNA) quantities between early onset cancer requiring intervention and non-lethal cancers or malignant cells that can be managed by the patient's immune system. (kaloramainformation.com)
Naive CD4 + T cells can develop into various helper T (Th) subsets with different cytokine profiles and play a discriminative role in translating antigen-specific immune responses into tissue functions or immunopathology. (atsjournals.org)
We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)
This will be coupled with next generation sequencing based approaches to understanding the consequences of perturbing immune cell function. (lu.se)

Furthermore, this system also allows to distinguish between hematopoietic cell autonomous and non-hematopoietic (mostly, thymus-intrinsic) causes of severe congenital T-cell lymphopenia. (nih.gov)
By flow cytometry and single cell RNA-sequencing, we further show that normal hematopoietic stem and progenitor cells lack detectable C3AR gene and protein expression, making it particularly suitable as a target for antibody therapy. (lu.se)
We also demonstrate that C3AR in combination with GPR56 distinguishes the leukemic stem cells (LSCs) in NPM1-mutated AML from the normal hematopoietic stem cells, defining the LSC population, as shown by transplantation into immunodeficient mice. (lu.se)

This technique requires that whole proteins or selected peptide antigens are added to blood cells, allowing the simultaneous analysis of both major histocompatibility complex class I and II restricted T-cell responses ( 7 ). (cdc.gov)
In this study, Pulsipher and colleagues investigated the predictive value of flow cytometry-a technique that interrogates proteins on the cell surface-and next-generation DNA sequencing minimal residual disease (NGS-MRD) monitoring, using blood and bone marrow samples collected from the ELIANA and ENSIGN phase II clinical trials at one, three, six, nine, and 12 months after tisagenlecleucel infusion. (aacr.org)
P. gingivalis HSP60 at a concentration of 10 ng/l significantly decreased the expression levels of VE‑cadherin and eNOS protein at 24 h stimulation, whereas no difference in these proteins was identified following a low dose of P. gingivalis HSP60 (1 ng/l). (spandidos-publications.com)
Understanding host proteins essential for IAV infection can identify targets for alternative host-directed therapies (HDTs). (cdc.gov)
Therefore, we aimed to identify cell surface proteins specifically expressed on NPM1)-mutated AML cells, allowing for potential targeting with antibody-based therapies. (lu.se)

As the two major cell types in the spleen, B cells and T cells produce cytokines and chemokines [ 18 ]. (hindawi.com)
Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
The overrepresentation of Th22 cells in TPE may be due to pleural cytokines and to PMC-produced chemokines. (atsjournals.org)
Our results suggest that the accumulation of Th22 cells in tuberculous pleural effusion may be due to the increased local proinflammatory cytokines and to pleural mesothelial cell-produced chemokines. (atsjournals.org)

Flow cytometry analyzes a population of cells on a cell-by-cell basis, a critical capability for today's researchers and clinicians who are looking for the very few cells among the many cells in a sample (like finding needles in a haystack) that will enable them to study a disease state or biological process. (bdbiosciences.com)
These capabilities have made flow cytometry a powerful tool with multiple applications for researchers and clinicians alike. (bdbiosciences.com)
This is the first paper to show an approach that identifies markers of relapse that are very specific, allowing clinicians to add additional therapy prior to relapse that will prevent it," said Michael Pulsipher, MD, lead author of the study and a professor of pediatrics and the division chief of Pediatric Hematology and Oncology at Intermountain Primary Children's Hospital and the Huntsman Cancer Institute of the University of Utah. (aacr.org)

Other studies in oral cancer [23,24] reported that there is no relationship between the histopathological grade of oral epithelial dysplasia or oral squamous cell carcinoma and the ploidy status. (researchgate.net)
Patients with proliferative verrucous leukoplakia (PVL) should avoid other known factors associated with development of oral squamous cell carcinoma, such as tobacco, alcohol, and betel. (medscape.com)

Using a rapid flow cytometric test, we previously showed that monitoring interferon (IFN)-γ production by antigen-pulsed T cells provides a quantitative and functional assessment of HIV- or cytomegalovirus (CMV)-specific CD8(+) and CD4(+) T cells ( 4 - 6 ). (cdc.gov)
He has also developed algorithms for identifying functional modules in protein interaction networks, and for identifying biomarkers for lung and prostate cancer from mass spectral data. (purdue.edu)
Overall, this gain-of-function approach using human cells uncovered functional transporter-nutrient relationships and revealed that transport activity up-regulation may be sufficient to overcome environmental metabolic restrictions. (life-science-alliance.org)
B-cell lymphomas (BCLs) constitute a diverse set of tially identify new functional, diagnostic, and therapeutic tumors, both morphologically and clinically, that are mainly targets. (lu.se)
Catenins form functional networks in the nucleus to regulate gene expression and determine cell fate. (bvsalud.org)
This study provides a comprehensive network model of IAV infection in human cells, identifying functional host targets for pan-viral HDT. (cdc.gov)

In response to these shortcomings, a cross-disciplinary effort has given birth to a new approach often termed „cytometry bioinformatics‟, to leverage complex computer algorithms and machine learning to automate analysis and improve the investigator‟s ability to extract meaning from high dimensional data. (biorxiv.org)

First, we cocultured dermal fibroblasts with dermal microvascular endothelial cells at a ratio of 2 : 1. (hindawi.com)
A fibrous sheet was formed by the interactions between the fibroblasts and the endothelial cells, and capillary-like structures were observed after 20 days of coculture. (hindawi.com)
ELISA demonstrated that vascularization was promoted by significant upregulation of vascularization associated growth factors due to interactions among the 3 types of cells in the bilayer, as compared to cocultures of fibroblast and endothelial cells and monocultures. (hindawi.com)
Most of these methods involve culturing endothelial cells on or within ECM substrates (e.g., collagen, fibrin, fibronectin, laminin, etc.) or other types of biomaterials to form capillary-like structures [ 15 ]. (hindawi.com)
These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
Certain studies show that P. gingivalis lipopolysaccharide (LPS) could induce the expression of intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 in human umbilical vein endothelial cells (HUVECs) ( 13 , 14 ), which significantly enhances trans-endothelial migration of inflammatory cells. (spandidos-publications.com)
[ 2 ] It has become clear that AILD is a clonal T-cell disorder involving deregulation of B-cells and endothelial cells against a background involving a unique malignant microenvironment. (medscape.com)

Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)

The fluorochrome-labeled cells are analyzed by flow cytometry, which categorizes individual cells according to size, granularity, fluorochrome, and fluorochrome intensity. (cdc.gov)

MRD was assessed by a quantitative four-color flow cytometry panel with a sensitivity level of 10 −4 . (haematologica.org)
Corticosteroids have a cytotoxic effect on lymphoma cells and can induce a radiographic response in up to half of patients, which limits the sensitivity of diagnostic tools like biopsy or lumbar puncture. (medscape.com)
In addition, we summarize data, presented in detail elsewhere, from a retrospective comparison of the sensitivity of flow cytometry with conventional cytogenetics for a large number of bone marrow aspirates on which both types of studies were performed. (nih.gov)

PDCs are a class of specialized dendritic cells that play a role in immunomodulatory functions and antigen presentation. (news-medical.net)
These monocytes isolation protocols can contribute to the development of reliable methods for the purification of human dendritic cells for research and clinical applications. (jove.com)
In addition, this is the first study to characterize the specific population of monocyte derived dendritic cells by single cell imaging flow cytometry. (jove.com)
The loss of TNF α receptor 1/2 resulted in reduced recruitment of neutrophils, macrophages, and dendritic cells into the spleen, but only neutrophil recruitment was inhibited in the peritoneal cavity. (hindawi.com)
Similarly, a lack of B cells significantly impeded the recruitment of neutrophils, macrophages, and dendritic cells to the spleen. (hindawi.com)
LT α is also required for the recruitment of dendritic cells, neutrophils, and macrophages to the mesentery in response to pristane [ 10 ]. (hindawi.com)
TNF α plays an important role in the formation of primary B cell follicles and follicular dendritic cells [ 16 ]. (hindawi.com)

We also identified metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), a known target lncRNA of METTL16, in these five GEO datasets. (bvsalud.org)

Epithelial cells were then seeded on the fibrous sheet to assemble the bilayered tissue. (hindawi.com)
Thus, the long-term survival and function of 3D tissues depend on the rapid development of new blood vessels to provide nutrients and oxygen to cells in the center of the tissue grafts. (hindawi.com)
Karolinska Institutet researchers have now identified T cells that contribute to the destruction of kidney tissue in patients with AAV and suggest the possibility of T cell screening to identify individuals at high risk. (reachmd.com)
Our finding of presence of GPR56+ CD4+ T cells only in kidneys with active inflammation suggest their contribution to local tissue destruction, which may reveal previously unrecognized disease mechanisms. (reachmd.com)
However, most GEP studies have typically been performed on whole tissue samples, containing varying degrees of tumor cell content, which results in uncertainties in data analysis. (lu.se)

They examined multiple markers of cell identity (CD22, CD123, CD303, and CD304) to determine the subgroups of PDCs. (news-medical.net)
Numerous markers for hematological cancer or cancer-related genomic DNA found in blood cells have been assayed. (kaloramainformation.com)
Herein, we performed an arrayed flow cytometry-based screen directed to 362 cell surface markers. (lu.se)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

Knowledge about signaling pathways in malignant cells may provide prognostic and diagnostic information in addition to identify potential molecular targets for therapy. (biomedcentral.com)
Therefore, signaling pathways that are required for normal B cell maturation and function are likely disturbed in SLL/CLL and MZL. (biomedcentral.com)
These signaling pathways include the signals propagated through the B-cell receptor (BCR), CD40 and cytokine receptors. (biomedcentral.com)
Queries of genes that impact mitochondrial function or pathways leading to transcription of lipid metabolism genes identified the fatty acid importer CD36 as highly expressed in Treg but not Teff from subjects with prediabetes. (diabetesjournals.org)
Gene expression profiling has clearly demonstrated that this disease entity is different from diffuse large B-cell lymphoma (DLBCL) and has identified several deregulated pathways involved in the pathogenesis of PMBCL. (medscape.com)
We used a mouse model of methylene diphenyl diisocyanate (MDI) asthma to identify biological pathways that may contribute to asthma pathogenesis. (cdc.gov)
Informatic software was used to identify biological pathways affected by respiratory tract exposure and potential targets for disease intervention. (cdc.gov)
A total of 7,928 SDE genes and 46 canonical pathways derived from it were identified. (frontiersin.org)
Ly6C high MC exhibited activated neutrophil degranulation, lysosome, cytokine production/receptor interaction and myeloid cell activation pathways, and Ly6C low MC presented features of lymphocyte immunity pathways in both mice. (frontiersin.org)
Twenty-four potential transcriptional regulatory pathways were identified based on SDE TFs matched with their corresponding SDE immunological genes. (frontiersin.org)

Human MC were initially divided into three subsets based on the cell surface expression of CD14 and CD16, and recently classified based on CD40 expression ( 2 - 5 ). (frontiersin.org)
Ongoing work in the laboratory is focused on testing the hypothesis that hypomorphic mutations in the RAG genes that partially (but not completely) compromise T-cell development cause altered cross-talk with thymic stromal cells and by that mechanism also affect maturation and function of subsets of thymic epithelial cells involved in the process of negative selection of self-reactive T cells, thereby leading to increased risk of autoimmunity. (nih.gov)
The capability of supervised classification algorithms in CytoPy to identify cell subsets was successfully confirmed by using the FlowCAP-I competition data. (biorxiv.org)
More and more data have demonstrated that several Th subsets, such as Th1 cells ( 3 ), Th17 cells ( 4 ), regulatory T cells ( 5 ), and so on, are involved in the pathogenesis of TPE. (atsjournals.org)
CD137+ alloreactive T cells were detected in different T cell subsets, including naive T cells, but were found preferentially in CD28+ T cells and not in the terminally differentiated T cell subset. (eur.nl)
CD4+ T-cells and other lymphocyte subsets in persons infected with human immunodeficiency virus (HIV). (cdc.gov)

Cell cycle analysis by DNA content measurement is a method that most frequently employs flow cytometry to distinguish cells in different phases of the cell cycle. (wikipedia.org)

B-cell receptor (BCR) and co-receptor CD40 signaling is essential for normal B cells, and there is increasing evidence that signaling via BCR and CD40 plays an important role in the pathogenesis of B-cell lymphoma. (biomedcentral.com)
The aim of this study was to investigate basal and induced signaling in lymphoma B cells and infiltrating T cells in single-cell suspensions of biopsies from small cell lymphocytic lymphoma/chronic lymphocytic leukemia (SLL/CLL) and marginal zone lymphoma (MZL) patients. (biomedcentral.com)
Similarly, CD40L-induced p-ERK and p-p38 were also significantly reduced in lymphoma B cells, whereas p-p65 (NF-κB) was equal to that of normal B cells. (biomedcentral.com)
Small lymphocytic lymphoma/chronic lymphocytic leukemia (SLL/CLL) and marginal zone lymphoma (MZL) are indolent lymphoid malignancies that arise from mature B cells. (biomedcentral.com)
Primary mediastinal B-cell lymphoma (PMBCL) is relatively rare B-cell non-Hodgkin lymphoma that comprises 6-12% of all diffuse large B-cell lymphomas (DLBCLs) and 2-4% of all non-Hodgkin lymphomas. (medscape.com)
Flow cytometry studies identify a unique immunophenotype profile for primary mediastinal B-cell lymphoma (PMBCL). (medscape.com)
The weak CD30 expression can aid in differentiating PMBCL from anaplastic large cell lymphoma and classic Hodgkin lymphoma. (medscape.com)
Several studies have explored the underlying genetic and molecular features that lead to the evolution of primary mediastinal B-cell lymphoma (PMBCL). (medscape.com)
Tisagenlecleucel is a chimeric antigen receptor T-cell (CAR-T) treatment-a type of therapy in which patients' own T cells are harvested, reprogrammed to target a protein called CD19 that is expressed on the surface of cancer cells, and returned to the patient to fight ALL and lymphoma. (aacr.org)
Primary central nervous system lymphoma (PCNSL) has been known by many other names, including reticulum cell sarcoma, diffuse histiocytic lymphoma, and microglioma. (medscape.com)
[ 6 ] Additionally, a biopsy of lymphoma pre-treated with corticosteroids may reveal only gliosis or lymphocytic and histiocytic infiltrates without identifiable neoplastic cells. (medscape.com)
AILD is a type of peripheral T-cell lymphoma that is clinically characterized by high fever and generalized lymphadenopathy. (medscape.com)
AILD-type dysplasia with an oligoclonal T-cell pattern has frequently been shown to progress into AILD-type lymphoma. (medscape.com)
Krenacs et al have suggested that the phenotype of neoplastic cells in angioimmunoblastic T-cell lymphoma appears consistent with the phenotype of activated follicular B-helper T cells. (medscape.com)
AILD, specifically angioimmunoblastic T-cell lymphoma, is mainly derived from CD2 + CD3 + CD4 + CD5 + CD7 - mature T-helper cells with varying expression and partial loss of detectable CD4. (medscape.com)
Dunleavy et al noted that overexpression of the chemokine CXCL13 and vascular endothelial growth factor-A in angioimmunoblastic T cell lymphoma suggests that it may be derived from follicular helper T cells. (medscape.com)
[ 6 ] The lymphomatous infiltrate in some bone marrow specimens from these AILD patients contained numerous small or scattered large B cells, and these cells resembled either benign lymphoid aggregates or T-cell-rich large B-cell lymphoma, respectively. (medscape.com)

Objective evidence of a myeloid stem cell neoplasm in the form of a clonal cytogenetic abnormality is provided in only 30% to 40% of the non-chronic myeloid leukemia (CML) chronic MPDs (non-CML MPDs) and in a similar percentage of the MDSs. (nih.gov)

In response to this there has been an increased effort to develop computational methodologies for handling high-dimensional single cell data acquired by flow or mass cytometry. (biorxiv.org)
Here we present CytoPy, a Python framework for automated analysis of high dimensional cytometry data that integrates a document-based database for a data-centric and iterative analytical environment. (biorxiv.org)

The group analyzed seven MN patients who presented with PDC proliferations comprising 5-26% of that blood cell or bone marrow. (news-medical.net)
Leukemia is a type of cancer that starts in immature blood cells in the bone marrow. (medicalnewstoday.com)
Your bone marrow makes the cells which will develop into white blood cells, red blood cells, and platelets. (medlineplus.gov)
When you have leukemia, your bone marrow makes large numbers of abnormal cells. (medlineplus.gov)
These abnormal cells build up in your bone marrow and blood. (medlineplus.gov)
CLL happens when there are changes in the genetic material (DNA) in bone marrow cells. (medlineplus.gov)
We retrospectively evaluated 4-color flow cytometry data from more than 400 bone marrow aspirates obtained since 1998 from patients suspected of having a non-CML MPD or an MDS. (nih.gov)
Comparing the cell surface expression on NPM1-mutated AML cells to primitive (CD34+ CD38-) normal bone marrow cells, we identified the complement receptor C3AR as specifically expressed in NPM1-mutated AML. (lu.se)

University of Oklahoma researcher publishes a study identifying 135 previously unknown pigmentation genes with broad implications for human diversity, cell biology and medicine. (ou.edu)
Their research has identified 135 new genes associated with pigmentation. (ou.edu)
To identify which genes influence melanin production, cells that lost melanin during the gene removal process needed to be separated from millions of other cells that did not. (ou.edu)
These separated cells were then analyzed to determine the identity of melanin-modifying genes. (ou.edu)
We identified both new and previously known genes that play important roles in regulating melanin production in humans. (ou.edu)
They further identified the function of two newly discovered genes: KLF6 and COMMD3. (ou.edu)
The technological processes developed and used by the research team could also be applied to identify genes that regulate melanin production in fungi and bacteria. (ou.edu)
Furthermore, the cell cycle regulatory genes p16 INK4a and p14 ARF are noted to be altered in lesions of proliferative verrucous leukoplakia. (medscape.com)
Whole exome sequencing of patients who experienced severe influenza reveals several genes, including scaffold protein AHNAK, with predicted loss-of-function variants that are also identified in our proteomic analyses. (cdc.gov)

When the abnormal cells crowd out the healthy cells, it can lead to infection, anemia , and easy bleeding . (medlineplus.gov)
The abnormal cells can also spread outside the blood to other parts of the body. (medlineplus.gov)
Flow cytometry allows scientists to rapidly analyze thousands of normal cells and identify the presence of rare and abnormal cells. (streck.com)
Abnormal DNA content has been related to aggressive behavior in Mucoepidermoid Carcinomas (MEC), Adenoid Cystic Carcinomas (AdCC), Acinic Cell Carcinomas (ACC) and oncocytomas (Onc) [8]. (researchgate.net)
These data indicate that more than 90% of non-CML MPD and MDS cases with a clonal cytogenetic abnormality will be identified as abnormal by 4-color flow cytometry, and they therefore validate the use of flow cytometry in the diagnosis of these disorders. (nih.gov)

The identified transcription factors influence both the global and specific gene expression of the BCLs and have possible implications for diagnosis and treat- ment. (lu.se)

Antigen stimulation via BCR in normal B cells initiates phosphorylation of the immunoreceptor tyrosine-based activation motifs (ITAMs) in the cytoplasmic tails of CD79a and CD79b. (biomedcentral.com)
Moreover, antigen-specific T-cell responses could be detected in exposed, but uninfected persons, as shown in those with HIV infection ( 3 ). (cdc.gov)
However, if a fluorochrome-tagged monoclonal antibody is bound to an antigen present on a cell, a fluorescent light signal will be picked up when that cell passes through the laser beam of the flow cytometer. (bdbiosciences.com)
Upon i.m. injection of DNA vectors expressing Nef mut -derivatives, nanovesicles containing antigens fused with Nef mut are released by muscle cells, can freely circulate into the body, and can be internalized by antigen-presenting cells (APCs). (nature.com)
The features of T cells recognizing the PR3 self-antigen are not known. (reachmd.com)
To identify normal patterns of antigen expression during myeloid maturation and to determine whether flow cytometric evaluation of myeloid maturation represents an additional objective way to assess the likelihood of a stem cell neoplasm. (nih.gov)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

We developed a CD8 + T-cell-based vaccine platform based on intramuscular (i.m.) injection of a DNA vector coding for antigens of interest fused at the C-terminus of a biologically inactive Human Immunodeficiency Virus (HIV)-Type 1 Nef protein (Nef mut ) having an unusually high efficiency of incorporation into EVs. (nature.com)
SIGNIFICANCE STATEMENT Astrocytes are traditionally identified immunohistochemically by antibodies that target cell-specific antigens in the cytosol or plasma membrane. (jneurosci.org)

A panel of 9 stimulation conditions targeting B and T cells, including crosslinking of the B cell receptor (BCR), CD40 ligand and interleukins in combination with 12 matching phospho-protein readouts was used to study signaling. (biomedcentral.com)
Characterization of human NK cells using the NK cell inhibitory receptor panel. (bdbiosciences.com)
Song et al identified a tumor suppressor gene that encodes the A-20 protein, which acts as an inhibitor to the NF-kappa-B pathway downstream from the tumor necrosis factor (TNF) receptor. (medscape.com)
Because the CD19 receptor is also expressed on normal B cells, treatment causes B-cell aplasia-the depletion of a patient's B cells. (aacr.org)
Here, our study shows that PR3 autoreactivity was enriched in CD4+T cells expressing the G-protein coupled receptor 56 (GPR56), which associates with cytotoxicity. (reachmd.com)
Ravi Kumar Sharma et al, Identification of proteinase 3 autoreactive CD4+T cells and their T-cell receptor repertoires in anti-neutrophil cytoplasmic antibodies associated vasculitis, Kidney International (2023). (reachmd.com)
Summary: Detection and isolation of viable alloreactive T cells at the single-cell level requires a cell surface marker induced specifically upon T cell receptor activation. (eur.nl)
In this study, a member of the tumour necrosis factor receptor (TNFR)-family, CD137 (4-1BB) was investigated for its potential to identify the total pool of circulating alloreactive T cells. (eur.nl)
T-cell numbers were derived from three measurements deter- attributable to the decrease in the number of T cells that bear mined with two different instruments, a hematology analyzer the CD4 receptor ( 5-9 ). (cdc.gov)

In both analysis types, all PDCs showed partial to moderate expression of the cell marker CD34 indicating premature PDCs. (news-medical.net)
Before analysis, the cells are usually permeabilised and treated with a fluorescent dye that stains DNA quantitatively, such as propidium iodide (PI) or 4,6-diamidino-2-phenylindole (DAPI). (wikipedia.org)
Cell cycle analysis was first described in 1969 at Los Alamos Scientific Laboratory by a group from the University of California using the Feulgen staining technique. (wikipedia.org)
The first protocol for cell cycle analysis using propidium iodide staining was presented in 1975 by Awtar Krishan from Harvard Medical School and is still widely cited today. (wikipedia.org)
Multiparameter analysis of the cell cycle includes, in addition to measurement of cellular DNA content, other cell cycle related constituents/features. (wikipedia.org)
Unless staining is performed using Hoechst 33342, the first step in preparing cells for cell cycle analysis is permeabilisation of the cells' plasma membranes. (wikipedia.org)
Since cells and especially fixed cells tend to stick together, cell aggregates have to be excluded from analysis through a process called doublet discrimination. (wikipedia.org)
Routine sample analysis rates can range up to 10,000 cells per second-an incredible advancement over historical methods of visually examining and counting cells. (bdbiosciences.com)
The analysis showed that SOX9+ astrocytes constitute ∼10-20% of the total cell number in most CNS regions, a smaller fraction of total cell number than previously estimated in the normal adult brain. (jneurosci.org)
Here, we report a surface enhanced Raman scattering technology for rapid and label-free exosomal detection (Exo-SERS) to aid in the discrimination of different cancer cells based on specific Raman phenotypes and multivariate statistical analysis. (mdpi.com)
Prior basic working knowledge of flow cytometry, data acquisition or analysis software (DIVA + FlowJo) is of advantage, but not strictly a must. (embl.org)
B ) Flow cytometry analysis of GFP+ rod photoreceptors and viability at different developmental stages. (elifesciences.org)
Cytometry analysis has grown in recent years with the expansion in the maximum number of parameters that can be acquired in a single experiment. (biorxiv.org)
Despite the success of numerous algorithms and published packages to replicate and outperform traditional manual analysis, widespread adoption of these techniques has yet to be realised in the field of cytometry. (biorxiv.org)
Cytometry data analysis has undergone a paradigm shift in response to the growing number of parameters that can be observed in any one experiment. (biorxiv.org)
To this end, a computational approach to analysis of cytometry data can take one of two strategies: to separate single cell data into groups or classifications, which then form the variables (often descriptive statistics of the obtained groups) the investigator uses to test their hypothesis, or directly model the acquired distribution of single cell data with respect to a chosen endpoint. (biorxiv.org)
SPT incorporates internal calibrator beads of known quantity in the analysis of specimens by three- or four-color flow cytometry. (cdc.gov)
Flow cytometry analysis of forebrain-derived and developing CNS in rodents (Rietze et al. (lu.se)

However, the incorporation of 5-ethynyl-2'-deoxyuridine (EdU), the precursor whose detection offers certain advantages over BrdU, has now become the preferred methodology do detect DNA replicating (S-phase) cells. (wikipedia.org)
PHILADELPHIA - In pediatric and young adult patients with acute lymphoblastic leukemia (ALL) treated with tisagenlecleucel (Kymriah), DNA sequencing-based detection of residual disease between three and 12 months accurately identified all patients who would eventually relapse, while other methods were less predictive, according to a study published in Blood Cancer Discovery , a journal of the American Association for Cancer Research. (aacr.org)
This work lays the foundation for future studies to identify biomarkers that differentiate WBCs from RBCs in label free strategies such as split-detection. (arvojournals.org)
Optimal conditions for sensitive and specific detection of allogeneic-induced CD137 expression on circulating T cells were established. (eur.nl)

PDC progenitors are in common with monocytic and myeloid cell progenitors. (news-medical.net)
Together, these findings provide insight into regulatory myeloid cell activities amplified by MIS416-mediated NOD-2 and TLR-9 signalling and highlight the potential importance of these cells in accessing the brain where they may act locally and contribute to the control of neuroinflammation. (nature.com)

BD Biosciences offers a range of research cell sorters for various research applications, from simple, benchtop systems to advanced, fully configurable systems. (bdbiosciences.com)

Regulatory T cells (Treg) control mitochondrial function and cytokine production by CD4 + effector T cells (Teff) in prediabetes and type 2 diabetes by supporting T helper (Th)17 or Th1 cytokine production, respectively. (diabetesjournals.org)
We conclude that Treg control CD4 + T cell cytokine profiles through mechanisms determined, at least in part, by host metabolic status. (diabetesjournals.org)
Upon allogeneic (re-)stimulation, the cytokine-producing as well as proliferative capacity of T cells resided mainly within the CD137-expressing fraction. (eur.nl)
Polyfunctional alloreactive T cells, defined by multiple cytokine expression, were observed infrequently. (eur.nl)

The cellular DNA content of individual cells is often plotted as their frequency histogram to provide information about relative frequency (percentage) of cells in the major phases of the cell cycle. (wikipedia.org)
The concurrent measurement of cellular DNA and RNA content, or DNA susceptibility to denaturation at low pH using the metachromatic dye acridine orange, reveals the G1Q, G1A, and G1B cell cycle compartments and also makes it possible to discriminate between S, G2 and mitotic cells. (wikipedia.org)
Cellular factors which play role in host defense mechanisms against HIV were identified and their role in HIV induced inflammation was established. (europa.eu)
HIV persistence may arise from ongoing residual virus replication and/or from latently-infected cells defined as the cellular reservoir in which long-lived resting memory CD4+ T cells harbouring an integrated but transcriptionally silent provirus represent the largest pool in the blood (Chomont et al. (europa.eu)
Among the transporters identified, we characterized the cationic amino acid transporter SLC7A3 as a gene that, when up-regulated, overcame low availability of arginine and lysine by increasing their uptake, whereas SLC7A5 was able to sustain cellular fitness upon deprivation of several neutral amino acids. (life-science-alliance.org)
Cellular immunity, particularly of CD4 + T cells, IFN-γ and tumor necrosis factor (TNF)-α, has a central role in the control of and protection against Mycobacterium tuberculosis (MTB) infection ( 1 ). (atsjournals.org)

This work builds on existing knowledge that PDCs can be divided into two categories: mature PDC proliferations which result in myeloid neoplasms (MPDMN) and blastic plasmacytoid dendritic cell neoplasm (BPDCN). (news-medical.net)

Of our identified host factors, 54 significantly alter IAV infection upon siRNA knockdown, and two factors, AHNAK and coatomer subunit COPB1, are also essential for productive infection by SARS-CoV-2. (cdc.gov)
Our objective is to identify the cells and factors that are essential in the engineering process. (lu.se)

PBMC were cultured in complete Roswell Park Memorial Institute 1640 medium, 10% v/v heat-inactivated fetal calf serum, 2 mM L-glutamine, and 10 U/mL penicillin/streptomycin at a concentration of 10 6 cells/mL. (cdc.gov)

Where cytometry is used for data acquisition, the typical objective is to discern differences between groups of subjects or experimental conditions, or to identify a phenotype that correlates with an experimental or clinical endpoint. (biorxiv.org)

Additionally, the malignant cells also lack CD15 expression but have frequent positivity of the transcriptional regulators BCL6, PAX5, and BOB1. (medscape.com)
Indeed, we were successful in identifying key regulators of HIV persistence and permissiveness. (europa.eu)

Drug discovery pipelines to identify drug candidates. (elifesciences.org)

[ 8 ] Tumor growth factor-alpha is a potent mitogenic polypeptide expressed by epithelial cells under physiologic conditions and by activated macrophages and eosinophils in certain pathologic conditions. (medscape.com)

The researchers found that, while flow cytometry could detect approximately one cancer cell per 10,000 blood cells, NGS-MRD was far more sensitive, with the ability to detect one cancer cell per 1 to 10 million blood cells, depending on the number of cells in the sample. (aacr.org)
The urgent need to improve the outcome of patients with various forms of cancer is boosting opportunities for new and existing tests that help detect tumors early, determine disease risk, identify and assess the effectiveness of therapies, and assist in the discovery and commercialization of new improved anti-cancer agents. (kaloramainformation.com)

Additionally, they developed a new technique for separating Mn-microparticles from sedimentary matrices by combining a sequential density concentration *4 and flow cytometry/particle sorting *5 , which enabled in-depth analyses of the texture and major and trace element compositions of Mn-microparticles. (go.jp)
Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses using three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions and identify 13 IAV-modulated kinases. (cdc.gov)

The cells in G1Q are quiescent, temporarily withdrawn from the cell cycle (also identifiable as G0), the G1A are in the growth phase while G1B are the cells just prior entering S, with their growth (RNA and protein content, size) similar to that of the cells initiating DNA replication. (wikipedia.org)

Th22 cells were significantly higher in TPE than in blood. (atsjournals.org)
CD95 counts in the 3 types of white blood cells were significantly higher in PEM infants and showed improvement after nutritional rehabilitation yet not reaching the control values. (who.int)
RT-qPCR and immunohistochemical staining confirmed that compared with the normal ovarian tissues and cells, METTL16 was significantly downregulated, while lncRNA MALAT1 was significantly upregulated, in 30 EOC tissues of our own validation cohorts and EOC cell lines, revealing a negative correlation between METTL16 and lncRNA MALAT1. (bvsalud.org)

First the parameters are set so that the flow cytometer can identify cells of interest. (bdbiosciences.com)
If a cell matches the parameters specified, the cytometer applies an electrical charge to it. (bdbiosciences.com)
that describe dual-platform technology, a method in which absolute counts are derived from measurements obtained from two instruments--a flow cytometer and hematology analyzer. (cdc.gov)

Measuring how much and in which direction light is scattered helps researchers determine both the size and internal complexity of a cell. (bdbiosciences.com)

To identify a nuclear marker pathognomonic of astrocytic phenotype, we assessed differential RNA expression by FACS-purified adult astrocytes and, on that basis, evaluated the expression of the transcription factor SOX9 in both mouse and human brain. (jneurosci.org)
We found that SOX9 is almost exclusively expressed by astrocytes in the adult brain except for ependymal cells and in the neurogenic regions, where SOX9 is also expressed by neural progenitor cells. (jneurosci.org)
Based on SOX9 immunolabeling, we document that astrocytes constitute a smaller fraction of total cell number than previously estimated in the normal adult mouse brain. (jneurosci.org)
A grant from the Oklahoma Center for Adult Stem Cell Research supported the study. (ou.edu)

Eosinophils in airway fluid were quantitated by flow cytometry. (cdc.gov)
Eosinophil Production and Function Eosinophils are granulocytes (white blood cells that contain granules in their cytoplasm) derived from the same progenitor cells as monocytes-macrophages, neutrophils, and basophils. (msdmanuals.com)

Samples from untreated SLL/CLL and MZL patients were examined for basal and activation induced signaling by phospho-specific flow cytometry. (biomedcentral.com)
Malignant B cells from SLL/CLL patients had higher basal levels of phosphorylated (p)-SFKs, p-PLCγ, p-ERK, p-p38, p-p65 (NF-κB), p-STAT5 and p-STAT6, compared to healthy donor B cells. (biomedcentral.com)
The group of benign tumours included 164 pleomorphic adenomas, 51 Warthin's tumours, 7 basal cell adenomas, 2 lipomas as well as 5 other different tumours. (researchgate.net)

Flow cytometry can help determine the type of leukemia. (medicalnewstoday.com)
Flow cytometry tests, which check for leukemia cells and identify which type of leukemia it is. (medlineplus.gov)

Whilst IFN-γ is typically considered to be a pro-inflammatory product of effector T cells, it has been also been demonstrated that IFN-γ −/− mice develop more severe EAE than wild type (WT) controls 5 , highlighting alternate, IFN-γ-dependant negative feedback mechanisms that can constrain EAE disease activity. (nature.com)
New data show that inflammatory profiles produced by CD4 + T cells define human prediabetes as a unique inflammatory state. (diabetesjournals.org)

Anatomy45

Organisms7

Diseases10

Chemicals and Drugs87

Analytical, Diagnostic and Therapeutic Techniques and Equipment56

Psychiatry and Psychology1

Phenomena and Processes60

Disciplines and Occupations4

Technology, Industry, Agriculture2

Information Science1

Health Care4

Populations11

Assays7

Vitro11

Multicolor flow cytometry3

Cytometric analysis4

Immunohistochemistry2

Mononuclear cells3

Lymphocytes11

Differentiation19

Tumor cells8

Proliferation6

Assay6

Vivo3

Immune11

Hematopoietic3

Proteins5

Cytokines4

Clinicians3

Oral squamous cell carc2

Functional6

Bioinformatics1

Endothelial cells7

Peripheral blood1

Fluorochrome-labeled1

Sensitivity3

Dendritic cells7

Lung1

Tissue5

Markers5

Pathways10

Subsets6

Distinguish1

Lymphoma17

Chronic1

High-dimensional2

Bone8

Genes9

Abnormal5

Gene expression1

Antigen7

Antigens2

Receptor9

Analysis18

Detection4

Myeloid cell2

Biosciences1

Cytokine4

Cellular6

Neoplasms1

Essential2

Concentration of 101

Data acquisition1

Regulators2

Pipelines1

Epithelial1

Detect2

Analyses2

Protein1

Significantly3

Cytometer3

Researchers1

Adult4

Eosinophils2

Basal3

Type of leukemia2

Inflammatory2