• A number of photoactivatable GFP-like fluorescent proteins (PAFPs) have been reported whose fluorescence can be switched on or whose fluorescent state can be modified by relatively intense irradiation at a specific wavelength. (nih.gov)
  • The use of these proteins gives unique opportunities to photolabel and track fusion proteins in a living cell. (nih.gov)
  • Both these proteins, PS-CFP2 and Dendra2, are fluorescent and can be visualized before photoactivation. (nih.gov)
  • Fluorescence activated cell sorting utilizes flow cytometry to separate cells based on morphological parameters and the expression of multiple extracellular and intracellular proteins. (wikipedia.org)
  • By taking advantage of this property of the dye, users can characterize intracellular activity as well as surface-expression of proteins. (wikipedia.org)
  • The cytotoxicity of different representative molecules, namely small organic molecules, proteins and nanoparticles (NP), was measured after 24 h of exposure using the fluorescent dye pair Hoechst 33342 and SYTOX orange. (nature.com)
  • Our results show that this dye pair can be distinctly measured in the presence of fluorescent proteins plus chlorophyll. (nature.com)
  • The main issue with trypan blue is its capacity to bind to proteins in the cell suspension, not just that released by dead cells. (nature.com)
  • Fluorophores for STORM and SMLM include synthetic dyes, fluorescent proteins (FPs), and even Quantum Dots (QDots). (microscopyu.com)
  • It can image DNA, chromatin and proteins in cells in their native states, without the need for labels. (news-medical.net)
  • For decades, textbooks have stated that macromolecules within living cells, such as DNA, RNA and proteins, do not have visible fluorescence on their own. (news-medical.net)
  • Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)
  • Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. (degruyter.com)
  • Estrogen and progesterone receptors are proteins in cells that attach to the hormones estrogen and progesterone to allow the cells to grow. (medicalnewstoday.com)
  • Analysis of live and fixed cells may be based on size, granularity/complexity, fluorescent proteins, cell surface antigen expression, intracellular antigen expression, DNA content. (lu.se)
  • Fluorescence-Activated Cell Sorting, is also known as flow cytometry cell sorting, or commonly known by the acronym FACS, which is a trademark of Becton Dickinson and Company. (wikipedia.org)
  • Flow cytometry cell sorting yields very high specificity according to one or several surface markers, but one limitation is constituted by the number of cells that can be processed during a work-day. (wikipedia.org)
  • Moreover, flow cytometry cell sorters are complex instruments that are generally used only by well-trained staff in flow cytometry facilities or well-equipped laboratories and, since they are normally big in size, it is not always possible to place them inside a biological safety cabinet. (wikipedia.org)
  • Fluorescent indicator dyes (fluorophores) are most commonly used in biochemical and cell-based assays in vertebrate cell culture since fluorescence is more sensitive 3 and fluorescence is utilized in many instruments (microscopy, spectroscopy, flow cytometry). (nature.com)
  • It was observed that tumor cells strongly accelerate plasma coagulation as a result of: i) expression of the blood clotting initiator protein, a tissue factor, as shown by flow cytometry and functional assays (factor Xa formation in the presence of cells and factor VIIa), and ii) direct activation of prothrombin to thrombin by cells, as evidenced by hydrolysis of the synthetic substrate, S-2238, and the natural substrate, fibrinogen. (scielo.br)
  • The labeled cells can be visualized using fluorescent imaging, quantified using flow cytometry, and isolated using fluorescence-activated cell sorting (FACS).The ALDEFLUOR™ fluorescent reagent system offers a novel approach to the non-immunological identification, evaluation and isolation of neural stem and progenitor cells. (stemcell.com)
  • The CDC guidelines concerning CD4+ T-cell determinations (33) were first published in the MMWR in 1992 to provide laboratorians with the most complete information about how to measure CD4+ T-lymphocytes in blood from HIV-infected persons by using flow cytometry. (cdc.gov)
  • The effects of ATP supplementation on the viability and apoptosis of HaCaT cells were determined by crystal-violet staining and flow cytometry, respectively. (bvsalud.org)
  • Flow cytometry enables measurement of fluorescence intensity of particles (cells) in a liquid stream. (lu.se)
  • Flow cytometry is ideal for multiplexing targets using multiple stainings and dyes. (lu.se)
  • Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)
  • Spectral imaging with polarity-sensitive fluorescent probes enables the quantification of cell and model membrane physical properties, including local hydration, fluidity, and lateral lipid packing, usually characterized by the generalized polarization (GP) parameter. (nih.gov)
  • This work presents the approach used to customize dye pairs for membrane integrity-based cell viability assays considering the species- and genotype-specific autofluorescence of scleractinian corals, namely: endogenous fluorescence characterization followed by the selection of dyes that do not overlap with endogenous signals. (nature.com)
  • It is important to systematically combine multiple endpoints, such as membrane integrity and cell death mechanism, to gain insight into the potential pathways involved in cellular toxicity. (nature.com)
  • Table 1 includes common fluorophores used to assess membrane integrity and distinguish between live and dead cells when conducting viability assays. (nature.com)
  • MitoTEMPO is a new cell permeable mitochondrial antioxidant with the functions of eliminating mitochondrial superoxide and preserving mitochondrial membrane potential [ 14 ]. (hindawi.com)
  • Cell membrane impermeable. (abcam.com)
  • Cell migration and invasion can be evaluated in vitro with widely-used membrane-based migration systems such as the Boyden Chamber assay. (sigmaaldrich.com)
  • This dye has high affinity for phosphatidylserine (PS) that is present in the inner leaflet of the plasma membrane. (stemcell.com)
  • During early-stage cell apoptosis, PS is translocated from the inner to the outer leaflet of the plasma membrane, exposing it to the external environment. (stemcell.com)
  • Therefore, as cells progress through apoptosis and towards necrosis, the cell membrane is compromised and consequently, viability dyes pass into the cell. (stemcell.com)
  • Membrane probes include fluorescent analogs of natural lipids, as well as lipophilic organic dyes that have little structural resemblance to natural biomolecules ( Figure 13.2.1 in Fatty Acid Analogs and Phospholipids-Section 13.2 ). (thermofisher.com)
  • Due to their low toxicity and stable retention, some lipid probes are particularly useful for long-term cell tracing ( Tracers for Membrane Labeling-Section 14.4 ). (thermofisher.com)
  • The inhibition of the intercellular communication appeared to be largely dependent on the ability of these compounds to partition in the cell membrane. (cdc.gov)
  • If you can identify which CD is presented on your cells of interest, then you can stain your sample with a fluorescent dye specific to it and use fluorescence-activated cell sorting to separate the population of interest. (wikipedia.org)
  • The use of trypan blue, a colorimetric, cell impermeant stain, to quantify cell death is the most reported method in relation to coral cell cultures despite its limitations. (nature.com)
  • Biologists use them to stain specific cell types, biochemists rely on them to monitor enzyme activity, and analytical chemists use them to detect molecules in the environment. (acs.org)
  • Other dyes can stain neurons but not selectively. (acs.org)
  • Thus, cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes, while apoptotic death or necrosis is characterized by positive staining for both Annexin V and the viability dye. (stemcell.com)
  • Once the cells have been identified via their nuclear stain in one fluorescence channel of an imaging system, their mitochondrial activity can be measured on a per-cell basis as fluorescence in a second channel corresponding to the specific mitochondrial dye used. (moleculardevices.com)
  • Cells were incubated for 30 minutes, and then the stain was removed. (moleculardevices.com)
  • In classical banding (eg, G [Giemsa]-, Q [fluorescent]-, and C-banding), a dye is used to stain bands on the chromosomes. (msdmanuals.com)
  • Select an area that is well-stained, free of stain precipitate, and well-populated with white blood cells (WBCs) (10-20 WBCs/field). (cdc.gov)
  • Fluorescent dyes that stain nucleic acids have been used in the detection of blood parasites. (cdc.gov)
  • HeLa cells were incubated with OptiMEM medium containing 10 uM FITC-LC-Antennapedia, cat# AS-24175, AS-24176 for 1 h, washed and analyzed by fluorescence microscopy. (anaspec.com)
  • It is commonly used to detect mycoplasma in cell culture via fluorescence microscopy. (sigmaaldrich.com)
  • Nanoscale imaging enables the analysis of biological systems, such as cells and tissues, at the fundamental length scales of biomolecules and biomolecular interactions, but classically has required expensive equipment and advanced skillsets to perform, e . g ., via super-resolution microscopy [ 1 ]. (plos.org)
  • Methods of cell sorting fall into two major categories: fluorescence activated cell sorting (FACS) and immunomagnetic cell sorting. (wikipedia.org)
  • Diagram A shows fluorescence-activated cell sorting of negative cell selection (undesired group) and diagram B shows FACS of positive cell selection (desired group). (wikipedia.org)
  • In this study, we developed a strategy for the isolation of polyphosphate-accumulating organisms (PAOs) by combining (i) nontoxic fluorescence staining of polyphosphate granules in viable microbial cells and (ii) fluorescence-activated cell sorting (FACS) for the rapid detection and collection of target cells. (frontiersin.org)
  • The polyP positive cells were recovered by FACS, purified by colony isolation and phylogenetically identified by 16S rRNA gene sequencing. (frontiersin.org)
  • Follow-up analysis confirmed that these isolates accumulate polyP, indicating that DAPI can be implemented in staining living cells and FACS can effectively and rapidly screen and isolate individual cells from a complex microbial community. (frontiersin.org)
  • In this study, we propose a strategy for the isolation of polyphosphate-accumulating organisms (PAOs) by coupling (i) specific staining of an intracellular accumulate without compromising cell viability, and (ii) rapid detection and sorting of target cells by using fluorescence-activated cell sorting (FACS), for which wastewater sludge samples were utilized. (frontiersin.org)
  • The FACS Aria may be used for analysis or for physically separating out cell populations based on their fluorescence labelling. (lu.se)
  • The application of established cell viability assays such as the commonly used trypan blue staining method to coral cells is not straightforward due to different culture parameters and different cellular features specific to mammalian cells compared to marine invertebrates. (nature.com)
  • These assays may be used to quantify and to assess the health of mammalian cell lines, primary cells and stem cells. (sigmaaldrich.com)
  • Backman, Zhang and Cheng Sun, an associate professor of mechanical engineering at McCormick, discovered that when illuminated with visible light, the biomolecules get excited and light up well enough to be imaged without fluorescent stains. (news-medical.net)
  • Jurkat T cells encapsulated with a double layer of PSS-GNR/PAH (PSS-GNR/PAH@Jurkat) showed the highest rate of cell proliferation when compared to 24-h encapsulated cells. (springer.com)
  • Rather than chemotherapy, Prof. Peer's nanoparticles contain nucleic acid with small interference RNAs, which silence the functioning of a key protein involved in cell proliferation. (eurekalert.org)
  • Cell viability and proliferation assays can be effectively used to evaluate cell health. (sigmaaldrich.com)
  • Cell viability and proliferation rate are often assessed using dyes such as trypan blue or Calcein-AM, or with fluorescent readouts. (sigmaaldrich.com)
  • Sphingolipids play critical roles in processes such as proliferation, apoptosis, signal transduction and molecular recognition at cell surfaces. (thermofisher.com)
  • Cell sorting is the process through which a particular cell type is separated from others contained in a sample on the basis of its physical or biological properties, such as size, morphological parameters, viability and both extracellular and intracellular protein expression. (wikipedia.org)
  • Using Pocillopora damicornis as a model, we characterized the autofluorescence and tested different fluorescent dye pair combinations to identify alternative viability indicators. (nature.com)
  • Annexin V is used along with viability dyes such as 7-AAD (Catalog #75001) or Propidium Iodide (Catalog #75002). (stemcell.com)
  • Res dose-dependently promoted the cell viability and protein levels of Bcl-2 (Linear P (bvsalud.org)
  • We are pleased to offer a broad range of premium fluorescent dyes that span the whole visible and near infrared spectrum, for incorporation into your custom peptides. (anaspec.com)
  • For peptides labeled with a dye and quencher, see our FRET peptides page. (anaspec.com)
  • We deliver fluorescent dye-labeled peptides in research grade or as critical raw material. (anaspec.com)
  • Cy3-labeled versions of both peptides were localized in the endothelial cells of arterial vessels in the rats. (go.jp)
  • The accumulation of both peptides in the endothelial cells suggested in vivo inhibitory activity of the angiotensin I-converting enzyme. (go.jp)
  • An IHC test uses antibodies to see if they attach to certain substances in cancer cells. (medicalnewstoday.com)
  • To implement this screening approach, cells from wastewater sludge samples were stained with 4'6-diamidino-2-phenylindole (DAPI) to target cells with high polyphosphate (polyP) accumulation. (frontiersin.org)
  • These are often used in conjunction with a nuclear counterstain, such as DAPI, which enables identification of all cells in the image. (moleculardevices.com)
  • DAPI is a fluorescent dye that binds selectively to double-stranded DNA and forms strongly fluorescent DNA-DAPI complexes with high specificity. (sigmaaldrich.com)
  • DAPI, once added to tissue culture cells, is rapidly taken up into cellular DNA, yielding highly fluorescent nuclei and no detectable cytoplasmic fluorescence. (sigmaaldrich.com)
  • Live Cell Imaging Platform at MultiParkNikon Ti-E is a fully motorised research microscope with Spectra X light engine and EMCCD, suitable for imaging of fluorescence labelling, such as DAPI, CFP, GFP, YFP, mCherry and Cy5. (lu.se)
  • Cellular assays, or cell-based assays, can be used in both biomedical research and drug-discovery screening applications to efficiently quantify cytotoxicity, biological activity, biochemical mechanisms and off-target interactions. (sigmaaldrich.com)
  • There is no other technology like it today-the peptide targets a variety of cancer cells with extreme precision, providing a binary approach to cancer diagnosis. (prweb.com)
  • NV-VPAC1 is a unique proprietary peptide construct that targets a specific receptor, called vasoactive intestinal peptide receptor type 1 (VPAC1), which is over-expressed on the surface of cancer cells. (prweb.com)
  • A peptide can be labeled with a single or multiple dye(s) at the peptide N-terminus, C-terminus, or internally, for applications in protein interaction studies , to trace the presence of biomolecules in cells, enzyme activity assays , etc. (anaspec.com)
  • Fluorogenic substrates do not require a quencher and contain a C-terminal dye that does not fluoresce until it is cleaved from the peptide (fluorescent form of dye is released). (anaspec.com)
  • Isolating and Expanding Endothelial Progenitor Cells from Peripheral Blood on Peptide‐functionalized Polystyrene Surfaces. (anaspec.com)
  • The most common way to measure these endpoints is by using colorimetric or fluorometric assays which involve the addition of an indicator dye. (nature.com)
  • The advantages of cell-based assays include the facilitation and generation of complex and biologically relevant data. (sigmaaldrich.com)
  • Unlike traditional biochemical assays, cell-based assays are more physiologically relevant and can assess compound characteristics simultaneously. (sigmaaldrich.com)
  • Explore tumor metastasis employing various cell migration and invasion assays like Boyden chamber assay, Millicell® µ-Migration Assay and in vitro scratch assay. (sigmaaldrich.com)
  • Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology. (sigmaaldrich.com)
  • Angiogenesis assays can be used to study both the induction and inhibition of angiogenesis in in vitro cell models. (sigmaaldrich.com)
  • In the liver of obesity, the resident immune cells such as macrophages (Kupffer cells) and dendritic cells produce proinflammatory chemokines and cytokines, including monocyte chemoattractant protein-1 (MCP-1), interleukin-1 β (IL-1 β ), and tumor necrosis factor- α (TNF- α ) in response to hepatocellular damage [ 10 - 12 ]. (hindawi.com)
  • The interaction of Ara h 2 with components of certain immune cells, referred to as dendritic cells, has not been investigated and could be key to understand the high potency of this allergen. (usda.gov)
  • Human monocyte-derived dendritic cells (MDDCs) were obtained and experiments of Ara h 2 internalization by MDDCs were carried out. (usda.gov)
  • The existing tools for spectral image processing, however, are insufficient for processing the large data sets afforded by this technological advancement, and are unsuitable for processing images acquired with rapidly internalized fluorescent probes. (nih.gov)
  • Analysis is demonstrated for cell membranes, cell-derived vesicles, model membranes, and microbubbles with environmentally-sensitive probes Laurdan, carboxyl-modified Laurdan (C-Laurdan), Di-4-ANEPPDHQ, and Di-4-AN(F)EPPTEA (FE), for quantification of the local lateral density of lipids or lipid packing. (nih.gov)
  • But the rational design of these fluorescent probes can be cumbersome. (acs.org)
  • One researcher in Singapore is developing probes in reverse, starting with synthesized fluorescent molecules and then finding the targets that they label. (acs.org)
  • The plasma membranes and intracellular membranes of live cells and the artificial membranes of liposomes represent a significant area of application for fluorescent probes. (thermofisher.com)
  • A FISH test uses specialized fluorescent dye and DNA to show changes in chromosomes and can also show levels of the HER2 gene. (medicalnewstoday.com)
  • Karyotype: The full set of chromosomes in a person's cells. (msdmanuals.com)
  • A karyotype analysis involves blocking cells in mitosis during metaphase and staining the condensed chromosomes. (msdmanuals.com)
  • Chromosomes from single cells are photographed, and their images are arranged, forming a karyotype. (msdmanuals.com)
  • He has spent over 30 years studying various aspects of the changes to chromosomes that lead a cell to change and develop into a cancer cell, with a particular focus on leukaemia. (lu.se)
  • Fluorescence "in situ" hybridization (FISH) technique enables chromosomes to be tagged with fluorescent dyes to highlight genetic traits. (lu.se)
  • Fluorescent small molecules are workhorses for scientists from many disciplines. (acs.org)
  • Prof. Peer devised a localized strategy to deliver RNA genetic interference (RNAi) directly to the tumor site using lipid-based nanoparticles coated with the polysugar hyaluronan (HA) that binds to a receptor expressed specifically on glioma cells. (eurekalert.org)
  • When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)
  • We expect nicotinamide riboside and other mitochondrial modulators to become a complementary approach for increasing stem cell fitness and accelerating blood production, either through dietary supplementation or pharmacological administration," says Naveiras. (eurekalert.org)
  • Mitochondrial activity can be readily analyzed using fluorescent dyes available in a variety of colors. (moleculardevices.com)
  • Valinomycin, an ionophore antibiotic, was used to disrupt mitochondrial function in PC12 cells, and MitoTracker Deep Red FM was used to monitor the effects. (moleculardevices.com)
  • Cells were imaged in the transmitted light (TL) channel of the MiniMax cytometer to obtain cell counts and assess morphology, and in the red fluorescent channel to measure mitochondrial activity. (moleculardevices.com)
  • For each cell identified, red fluorescent signal representing mitochondrial staining was measured and captured as an output parameter in the software. (moleculardevices.com)
  • Since the resulting nuclear identification masks do not include the cytoplasmic area where mitochondrial fluorescent staining occurs, the masks were extended or 'grown' outward by 4 pixels, or 7.5 µm. (moleculardevices.com)
  • For each analysis case, mitochondrial response to compound treatment was graphed as average mitochondrial fluorescence per cell vs. compound concentration, and IC 50 values were calculated automatically by the software. (moleculardevices.com)
  • PC12 cells showed a concentrationdependent reduction in mitochondrial labeling when treated with valinomycin. (moleculardevices.com)
  • Cellular and mitochondrial ROS were stained using fluorescent dyes. (bvsalud.org)
  • In contrast, the Northwestern technique, called spectroscopic intrinsic-contrast photon-localization optical nanoscopy (SICLON), allows researchers to study biomolecules in their natural environment, without the need for these fluorescent labels. (news-medical.net)
  • When excited with the right wavelength, the biomolecules even light up better than they would with the best, most powerful fluorescent labels. (news-medical.net)
  • Jesper Nygård has worked with biologists on interfacing nanowires with biomolecules and living cells. (lu.se)
  • I want to get the latest chemistry news from C&EN in my inbox every week. (acs.org)
  • This method allows multiparameter cell sorting and involves encapsulating cells into small liquid droplets which are selectively given electric charges and sorted by an external electric field. (wikipedia.org)
  • Excluding genetic constructs cloning, cell culturing and transfection, the whole protocol may take anywhere from 10 min to several hours, depending on motility of the protein being studied. (nih.gov)
  • Backman, Zhang and collaborators now are using the label-free technique to study chromatin -- the bundle of genetic material in the cell nucleus -- to see how it is organized. (news-medical.net)
  • Current technology for imaging DNA and other genetic material relies on special fluorescent dyes to enhance contrast when macromolecules are imaged. (news-medical.net)
  • Sequencing of DNA gives a direct picture of our genetic make-up, while sequencing of the RNA that is naturally present in cells gives a picture of which parts of our non-coding DNA are used and in what way. (lu.se)
  • Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. (sigmaaldrich.com)
  • Learn how to set up a T cell killing assay using Millicell ® Microwell plates. (sigmaaldrich.com)
  • Cell-based assay development for drug discovery can accelerate optimization of treatments for cancer and other angiogenesis-dependent diseases. (sigmaaldrich.com)
  • MATERIALS AND METHODS: The toxicity of ATP on HaCaT cells was examined by the MTT assay. (bvsalud.org)
  • [ 2 ] This technique has evolved throughout the years and is now largely performed by biopsy of the blastocyst trophectoderm cells with analysis using techniques such as next-generation sequencing (NGS) and comparative genomic hybridization (CGH) to test for aneuploidy. (medscape.com)
  • A doctor may also use a fluorescence in situ hybridization (FISH) test to check HER2 levels in cancer cells. (medicalnewstoday.com)
  • Expression of Bax, B-cell lymphoma (Bcl)-2, sirtuin (SIRT)3, and superoxide dismutase (SOD)2 was measured via western blotting. (bvsalud.org)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • Due to many years of refinement and increased demand for cell separation however, researchers are working to develop microfluidic sorting devices that have many benefits in comparison to the main types of fluorescence-activated cell sorting and immunomagnetic cell sorting methods. (wikipedia.org)
  • Fluorescence activated cell sorting has several systems that work together to achieve successful sorting of events of interest. (wikipedia.org)
  • Then, their urine specimens were examined under a confocal microscope-remarkably, the researchers noted that prostate cancer cells fluoresced under the microscope, while healthy, normal cells did not. (prweb.com)
  • The multiple imaging modes afforded by widefield, confocal, and multiphoton fluorescence microscopies permit noninvasive, temporally resolved imaging of fixed and living cells and tissues with a high level of biochemical specificity. (microscopyu.com)
  • Confocal Microscope Platform at MultiParkLeica SP8 confocal microscope for imaging of fixed tissue and cells. (lu.se)
  • Generally, a fluorescent dye will be excited by a light source (a laser) at a particular wavelength and emit light at a lower energy and longer wavelength. (wikipedia.org)
  • This reagent labels active mitochondria in live cells and fluoresces at a far-red wavelength that is detected by the red fluorescent channel of the MiniMax cytometer. (moleculardevices.com)
  • Such imaging is vital for mapping the brain and requires differentiating between the brain's cell types, including neurons, microglia, oligodendrocytes, and astrocytes. (acs.org)
  • Provided here is an in-depth discussion of STORM, and more generally SMLM, with attention to multichannel, three-dimensional, and live cell imaging approaches, and a special focus on the critical steps of fluorophore selection, labeling strategies, and imaging buffer formulation. (microscopyu.com)
  • StainFree ™ Technology from Molecular Devices frees researchers from the inconvenience of nuclear counterstaining by identifying cells imaged with the transmitted light channel of the SpectraMax ® MiniMax ™ 300 Imaging Cytometer. (moleculardevices.com)
  • Here, fluorescently stained cell nuclei were identified in the green fluorescence imaging channel. (moleculardevices.com)
  • Fluorescence microscopes and live cell imaging for microbiology at Dept. of Biology. (lu.se)
  • Cells were seeded at 10,000 cells per well at 100 µL per well, in 96-well black-wall, clear-bottom plates. (moleculardevices.com)
  • More recently, a number of studies have focused on the procoagulant mechanisms exhibited by tumor cells. (scielo.br)
  • Moreover, a monoclonal antibody against bovine factor Xa reduced the prothrombin-converting activity of tumor cells. (scielo.br)
  • Then we injected our designed particle with fluorescent dye to monitor its success entering the tumor cells. (eurekalert.org)
  • We were pleased and astonished to find that, a mere three hours later, the particles were situated within the tumor cells. (eurekalert.org)
  • Metastasis is the cumulative result of multiple changes in tumor cells. (sigmaaldrich.com)
  • Cytotoxic activity of a-humulene and transcaryophyllene from Salvia officinalis in animal and human tumor cells. (academicjournals.org)
  • People may have additional treatments to shrink a tumor before surgery or destroy any remaining cancer cells after surgery. (medicalnewstoday.com)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
  • Immunoprecipitation of mTOR protein from MCF-7 cell extracts. (cellsignal.com)
  • After 2 days, the cells were harvested, washed, and restimulated with Phorbol 12-Myristate 13-Acetate and Ionomycin in the presence of BD GolgiStop™ Protein Transport Inhibitor (Cat. (bdbiosciences.com)
  • This protein is not expressed in normal cells, so it only works where cells are highly proliferated. (eurekalert.org)
  • Some types of breast cancer cells use estrogen and progesterone as well as a protein called HER2 to grow. (medicalnewstoday.com)
  • Knowing whether cancer cells have estrogen or progesterone receptors, and the level of HER2 protein, can help determine the best course of treatment. (medicalnewstoday.com)
  • HER2 is a protein that allows breast cancer cells to grow quickly . (medicalnewstoday.com)
  • However, there are many other mechanisms by which fluorescent dyes can act. (wikipedia.org)
  • In the present study, we dissected the molecular mechanisms responsible for the procoagulant activity of MV3, a highly aggressive human melanoma cell line. (scielo.br)
  • The fluorochrome-labeled cells are analyzed by using a flow cytometer, which categorizes individual cells according to size, granularity, fluorochrome, and intensity of fluorescence. (cdc.gov)
  • Annexin V, a characteristic marker for early cell apoptosis, detects the translocation of PS to the external environment. (stemcell.com)
  • Resveratrol (Res) is a bioactive dietary component and alleviates apoptosis in multiple cell types. (bvsalud.org)
  • However, its effect and mechanism on lipopolysaccharide (LPS)-induced bovine mammary epithelial cells (BMEC) apoptosis, which commonly happens in dairy cows with mastitis, is unknown. (bvsalud.org)
  • Fig. 7: Characterization of the specificity of T cell clones cross-reactive against nontyphoidal Salmonella . (nature.com)
  • Medium affinity green fluorescent calcium binding dye. (abcam.com)
  • High affinity, green fluorescent Ca 2+ binding dye. (abcam.com)
  • Whether you're starting from primary cells, tissue samples, or pluripotent stem cells, explore our complete portfolio of products to support your neural culture workflow. (stemcell.com)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)
  • Cells were harvested and labeled with FITC Annexin V and 7-AAD. (stemcell.com)
  • When the cells are contaminated with Mycoplasmas, characteristic discrete fluorescent foci are readily detected over the cytoplasm and sometimes in intercellular spaces. (sigmaaldrich.com)
  • Certain fluorescent fatty acids ( Fatty Acid Analogs and Phospholipids-Section 13.2 ) are readily metabolized by live cells to phospholipids, mono-, di- and triacylglycerols, cholesteryl esters and other lipid derivatives. (thermofisher.com)
  • We offer fluorescent and, in a few cases, biotinylated analogs of five naturally occurring lipid classes-phospholipids, sphingolipids (including ceramides), fatty acids, triglycerides and steroids. (thermofisher.com)
  • Fluorescent phospholipid analogs ( Fatty Acid Analogs and Phospholipids-Section 13.2 ) can be classified according to where the fluorophore is attached. (thermofisher.com)
  • Together, these findings establish SIRT3 as a regulator of lipid metabolism and potential therapeutic target in primitive AML cells. (bvsalud.org)
  • Simple Western™ analysis of lysates (0.1 mg/mL) from Hela cells using mTOR (7C10) Rabbit mAb #2983. (cellsignal.com)
  • Simple Western™ analysis of lysates (1 mg/mL) from HeLa cells using AMPKα (D5A2) Rabbit mAb #5831. (cellsignal.com)
  • Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® mTOR siRNA II (+), using mTOR (7C10) Rabbit mAb #2983 and α-Tubulin (11H10) Rabbit mAb #2125. (cellsignal.com)
  • Uptake of CPP by HeLa cells. (anaspec.com)
  • Some dyes are able to diffuse across membranes. (wikipedia.org)
  • Phospholipids are the principal building blocks of cell membranes. (thermofisher.com)
  • During cell invasion, cells can modulate the environment as they migrate through the structure of the extracellular matrix (ECM). (sigmaaldrich.com)
  • The complete blood count (CBC) is a test that measures the levels of red blood cells, white blood cells, and platelets in your blood. (cancer.org)
  • They may have shortages of white blood cells and blood platelets as well. (cancer.org)
  • Platelets, which are cells that clot the blood. (healthline.com)
  • Plate Runner Platform at MultiParkHigh content screening (HCS) / Analysis (HCA) enables automated acquisition, visualization and quantification of a large number of images such as rapid snapshot acquisition of fluorescence images of cells and small organisms. (lu.se)
  • Stem cell-based therapies are becoming more and more common, especially in the treatment of blood cancers like lymphoma and leukemia. (eurekalert.org)
  • In these cases, the patient's cancerous blood stem cells are removed and replaced with new, healthy ones. (eurekalert.org)
  • these are the stem cells that produce the various types of blood cells in our bodies - red, white etc. (eurekalert.org)
  • The study, which also involved labs from EPFL's Institute of Bioengineering, and the University hospital of Lausanne (CHUV) has significant implications for stem-cell therapy patients, especially since nicotinamide riboside can be taken as a dietary supplement and still have such effects. (eurekalert.org)
  • Cell Stem Cell 07 March 2019. (eurekalert.org)
  • Primary antibodies are available for the characterization of neural stem cells, neural progenitor cells and their progeny. (stemcell.com)
  • Outcomes for patients with acute myeloid leukemia (AML) remain poor due to the inability of current therapeutic regimens to fully eradicate disease-initiating leukemia stem cells (LSC). (bvsalud.org)
  • Using RNAi and a SIRT3 inhibitor (YC8-02), we demonstrate that SIRT3 is a critical target for the survival of primary human LSC but is not essential for normal human hematopoietic stem and progenitor cell function. (bvsalud.org)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
  • Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
  • Green fluorescent Ca 2+ indicator. (abcam.com)
  • By synthesizing GaP GaInP nanowire heterostructures, with nonfluorescent GaP segments and fluorescent GaInP segments, we created a barcode labeling system enabling the distinction of the nanowire morphological and chemical properties using fluorescence. (lu.se)
  • 5. 293T cells - must never be grown to confluency. (lu.se)
  • Flow cytometric analysis of Jurkat cells using Akt (pan) (C67E7) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype control #3900 (dashed line). (cellsignal.com)
  • Analyses using StainFree and fluorescent nuclear dye to identify the cells were compared and results evaluated using SoftMax ® Pro Software. (moleculardevices.com)
  • In the first, individual cells were identified using StainFree analysis, which allows identification of individual cells in the transmitted light images without the need for fluorescent nuclear staining. (moleculardevices.com)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)