Cells dna probes. Medical search. Frequent questions

Recently, we have demonstrated high-bandwidth detection of double-stranded (ds) DNA with microsecond time resolution in nanopipettes, using custom-designed electronics. (imperial.ac.uk)
It involves randomly nicking the backbone of a double-stranded DNA with dilute concentrations of DNase I. At extremely low concentrations, this enzyme nicks a template at four or five sites, producing a free 3′-OH group that can act as a primer at each nicking location. (enzolifesciences.com)
DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. (sigmaaldrich.com)
It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. (sigmaaldrich.com)
DAPI is a fluorescent dye that binds selectively to double-stranded DNA and forms strongly fluorescent DNA-DAPI complexes with high specificity. (sigmaaldrich.com)
Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone. (sigmaaldrich.com)
Phenantridine alkaloids such as sanguinarine, chelerytrine, chelirubine, chelilutine, sanguilutine and macarpine interact with double stranded DNA showing substantial changes in fluorescence emission. (muni.cz)
The response of luminescence intensity to low concentrations of double stranded DNA in presence of alkaloids was found to be linear and can be potentially used for quantification of DNA by means of luminescence spectrometry. (muni.cz)
The new shape looks entirely different to the double-stranded DNA double helix. (sciencedaily.com)
When bound to double-stranded DNA, DAPI has an absorption maximum at a wavelength of 358 nm ( ultraviolet ) and its emission maximum is at 461 nm (blue). (wikipedia.org)
After a series of successive cycles of amplification, the presence of double stranded DNA product was visualized on agarose gels stained with ethidium bromide. (cdc.gov)
HPVs are small non-enveloped, double-stranded, circular DNA viruses that encode two viral oncoproteins, E6 and E7. (bvsalud.org)

Joanna then worked as a postdoctoral researcher with Professor Benedikt Kessler in the Department of Medicine, where she created novel ubiquitin-based covalent capture probes and developed inhibitor screening assays. (tcd.ie)
She returned to chemistry department at the University of Oxford in 2014 to work with Professor Tom Brown where her research focused on the study of DNA cross-link repair enzymes, developing assays to study their selectivity and for inhibitor screening. (tcd.ie)
Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
DNA/RNA probe assays are faster and sensitive so that many conventional diagnostic tests for viruses and bacteria involving culturing of the organisms are being fast replaced by molecular probe assays. (enzolifesciences.com)
While culture tests can take days, molecular probe assays can be performed within a few hours or minutes. (enzolifesciences.com)
In gel shift assays this antibody supershifts complexes of Fli-1 and DNA probe. (bdbiosciences.com)
Besides premade probes, also genome wide, custom Pinpoint FISH assays engineered to meet your specific requirements are offered. (biocat.com)
This greatly enhanced the sensitivity of assays used to detect that specific sequence of DNA. (cdc.gov)

Specific molecular probes and primers are designed for this purpose. (enzolifesciences.com)
Molecular probes can be broadly categorized into DNA probes and RNA probes, cDNA probes, and synthetic oligonucleotide probes. (enzolifesciences.com)
He and his colleagues developed molecular probes that attach to proteins of interest and have DNA tags extending outward. (eurekalert.org)
A technique in which electric pulses, in kilovolts per centimeter and of microsecond-to-millisecond duration, cause a loss of the semipermeability of CELL MEMBRANES, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA. (bvsalud.org)

We demonstrate how HyPR-seq can profile the effects of CRISPR perturbations in pooled screens, detect time-resolved changes in gene expression via measurements of gene introns, and detect rare transcripts and quantify cell-type frequencies in tissue using low-abundance marker genes. (nsf.gov)
MLPA can be used to detect tumours in the glial cells of the brain, as well as chromosomal abnormalities. (wikipedia.org)
FISH can also be used to detect and localize specific RNA targets in cells, circulating tumor cells, and tissue samples. (biogenex.com)
It is commonly used to detect mycoplasma in cell culture via fluorescence microscopy. (sigmaaldrich.com)
To detect the i-motifs inside cells, the researchers developed a precise new tool -- a fragment of an antibody molecule -- that could specifically recognise and attach to i-motifs with a very high affinity. (sciencedaily.com)
Crucially, the antibody fragment didn't detect DNA in helical form, nor did it recognise 'G-quadruplex structures' (a structurally similar four-stranded DNA arrangement). (sciencedaily.com)
Outside of analytical fluorescence light microscopy DAPI is also popular for labeling of cell cultures to detect the DNA of contaminating Mycoplasma or virus . (wikipedia.org)
In 2005, the San Diego, California-based biotech company Sequenom licensed technology to detect fetal DNA, building on the results of a 1997 Lancet study that found fetal DNA circulating in maternal blood. (the-scientist.com)
The Pinpoint FISH TP53/CEP 17 Probe Kit is intended to detect the copy number of the LSI TP53 probe target located at chromosome 17q11.1 and of the CEP 17 probe target located at the centromere of chromosome 17. (biocat.com)
The test strips prepared with this probe could be applied to detect mercury ions in aqueous solutions. (bvsalud.org)
In addition, the probe Coa-SH provided a tool to detect Hg2+ in living systems. (bvsalud.org)

We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. (nature.com)
Glass or quartz nanopipettes have found increasing use as tools for studying the biophysical properties of DNA and proteins, and as sensor devices. (imperial.ac.uk)
Labeling is possible in bacterial as well as eukaryotic cells and is independent of the nature of the label, thereby opening up new ways to study proteins in vivo. (chimia.ch)
SPY555-DNA stains the nuclei of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. (cytoskeleton.com)
Like other ets-related proteins it binds to consensus sites in DNA through a 85 amino acid ets domain in the carboxyl region of the protein. (bdbiosciences.com)
image: Molecular methods developed by Prof. Savas Tay let researchers use high-throughput genomic sequencing to measure proteins, protein complexes and mRNA in individual cells. (eurekalert.org)
Today, most methods to determine the proteins inside a cell rely on a crude census-scientists usually grind a large group of cells up before characterizing their genetic material. (eurekalert.org)
Now, researchers at the University of Chicago's Pritzker School of Molecular Engineering (PME) have developed an approach that lets them more easily study whether proteins are located in close proximity to each other inside a cell. (eurekalert.org)
By sequencing all of a cell's mRNA molecules-which encode proteins-they can get an idea of what proteins a cell might be actively using. (eurekalert.org)
Just knowing the abundance of certain proteins doesn't always give you information about how a cell is functioning," said Tay. (eurekalert.org)
If two proteins are physically close to each other, those DNA probes stick together like Velcro. (eurekalert.org)
The researchers can set up experiments that simultaneously probe hundreds of proteins in this way. (eurekalert.org)
Then, they use routine sequencing methods to read back any DNA probes that bound to each other and determine which proteins were paired up. (eurekalert.org)
In the vast space inside a cell, it's very unlikely for two probes to find each other unless they are bound to nearby proteins," said Tay. (eurekalert.org)
So when the probes bind, that tells us these proteins are very close together. (eurekalert.org)
It also could identify previously unknown subsets of the cells based on small differences in how groups of proteins were organized within the cells. (eurekalert.org)
Using human derived peripheral blood mononuclear cells, they simultaneously measured 38 individual proteins, 741 protein complexes, and thousands of mRNA on each individual cell, and discovered a new protein complex that defines naïve T cells. (eurekalert.org)
Then, the group used Prox-seq to study how proteins change arrangement in macrophages, another type of immune cell, when the cells become activated in response to a pathogen. (eurekalert.org)
they're now working to expand it to more types of proteins as develop ways to resolve exactly where within a cell proteins are interacting. (eurekalert.org)
The quest to understand the workings of human cells will not be over until we understand how this genetic blueprint is used to produce a particular set of proteins - the proteome - for each type of cell and how these proteins control the physiology of the cell. (learner.org)
These viral proteins also interact with other several cellular factors and lead to revoke normal cell cycle checkpoint stand cell death mechanisms 13 . (bvsalud.org)

Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. (sigmaaldrich.com)
DAPI, once added to tissue culture cells, is rapidly taken up into cellular DNA, yielding highly fluorescent nuclei and no detectable cytoplasmic fluorescence. (sigmaaldrich.com)

Lastly, EHMT1 sequencing is a process in which a single-strand of DNA from the EHMT1 gene is removed, and DNA polymerase is added in order to synthesize complementary strands. (wikipedia.org)
The annealed primers ultimately become part of the probe itself, because the Klenow fragment of DNA polymerase I extends the primers in the 3′ direction and, in so doing, incorporates the label. (enzolifesciences.com)
Next, the enzyme DNA polymerase I removes the native nucleotides from the probe molecules in the 5′→3′ direction (exonuclease activity) while replacing them with labeled dNTP precursors by virtue of its 5′→3′ polymerase activity. (enzolifesciences.com)
LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. (neb.com)
This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. (neb.com)
Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. (neb.com)
During PCR testing, Taq polymerase and DNA primers complementary to a unique sequence of target DNA were used to greatly amplify that region if the target was present in the sample. (cdc.gov)
We compared the performance of stool microscopy with the highly specific real-time polymerase chain reaction (PCR) we recently described for the detection and quantification of parasite-specific DNA. (cdc.gov)

Ap-ha strain from the Ap-variant 1 strain of A. phagocy- he gram-negative bacterium Anaplasma phagocytophi- tophilum lum based on DNA sequence comparisons of the 16S is the causative agent of human granulocytic ana- rRNA gene of each strain (online Technical Appendix 1) plasmosis (HGA) in the United States ( 1 ). (cdc.gov)
HGA represents an emerging disease DNA sequence of the Ap-variant 1 strain was associated in southern Canada because populations of I. scapularis with a restriction site for the endonuclease Kpn2I (T/CC- ticks have become established or are in the process of be- GGA) that was absent in the sequence of the Ap-ha strain. (cdc.gov)
Single-cell sequencing has made considerable progress relying on the notion that the sequence is the tag. (nature.com)
In turn, this allows scientists to map out a person's DNA sequence allowing for a diagnosis to be made. (wikipedia.org)
Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)
In developing a probe, a sequence of nucleotides must be identified, isolated, reproduced in sufficient quantity, and tagged with a label that can be detected. (enzolifesciences.com)
In theory, any nucleic acid can be used as a probe provided it can be labeled to permit detection and quantitation of the hybrid molecules formed between the probe and sequence to be identified. (enzolifesciences.com)
A DNA probe is a labeled fragment of DNA that contains a nucleotide sequence specific for the gene or chromosomal region of interest. (enzolifesciences.com)
Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for the detection of chromosomal aberrations, presence or absence of specific DNA sequence in native context. (biogenex.com)
In this technique florescent probes bind to the target sequence of DNA in chromosome. (biogenex.com)
DNA sequencing and sequence similarity analysis identified one of the aberrantlyl methylated DNA fragments as the p16 tumor suppressor gene. (cdc.gov)
Dulbecco also argued that such a project should be "an international undertaking, because the sequence of the human DNA is the reality of the species, and everything that happens in the world depends upon those sequences. (learner.org)
The HBV sequence was then labeled for its utilization as a probe in the HBV DNA detection in serum of patients with HBsAg positive, with no evident physical symptoms of the disease. (bvsalud.org)

Tests are either conducted at birth, or later in early childhood via: fluorescence in situ hybridization (FISH), multiplex ligation-dependent probe amplification (MLPA), array comparative genomic hybridization (aCGH), and EHMT1 sequencing. (wikipedia.org)
FISH is a screening test that uses multicolour probes or comparative genomic hybridization to find any chromosome irregularities in a genome. (wikipedia.org)
Array-based comparative genomic hybridization (aCGH) tracks chromosome deletions and or amplifications using fluorescent dyes on genomic sequences of DNA samples. (wikipedia.org)
Genomic DNA isolated from BALB/c-3T3 cells morphologically transformed with Cd was restriction digested with Mse1 (methylation non-sensitive) alone or with MSe1 and BstU1 (methylation sensitive). (cdc.gov)
KromaTiD sub-CEPs make use of pericentric non-repetitive genomic DNA to provide a uniform signal from chromosome to chromosome. (biocat.com)
The reactions were performed using a primer pair (TCZ1 and TCZ2) which prime the amplification of a highly repetive 195-bp T. cruzi genomic DNA. (bvsalud.org)

Bucevičius J, Gerasimaitė R, Kiszka KA, Pradhan S, Kostiuk G, Koenen T, Lukinavičius G (2022) A general, highly efficient and facile synthesis of biocompatible rhodamine dyes and probes for live-cell multicolor nanoscopy. (uni-goettingen.de)
SPY555-DNA is a bright & non toxic live cell nuclear stain based on our SPYTM dyes series. (cytoskeleton.com)
In conclusion, we have demonstrated that natural products, phenantridine alkaloids, can be used as alternative probes to synthetic DNA dyes such as ethidium bromide. (muni.cz)
Furthermore, to demonstrate the applicability of GX dyes, the first NIR-II PA probe GX-5-CO activated by carbon monoxide (CO) was engineered and employed to reveal the enhancement of the CO levels in the hypertensive mice by high-contrast NIR-II PA and FL imaging. (bvsalud.org)
We expect that many derivatives of GX dyes will be developed to afford versatile NIR-II PA platforms for designing a wide variety activatable NIR-II PA probes as biomedical tools. (bvsalud.org)

As each chromosome contains a single centromere, it remains unclear how acentric fragments derived from shattered chromosomes are inherited between daughter cells during mitosis 6 . (nature.com)
The stochastic inheritance of chromosome fragments by both newly formed daughter cells could in part contribute to the alternating DNA copy-number states that are characteristic of chromothripsis 2 . (nature.com)
One special type of plasmid used for genome sequencing is a bacterial artificial chromosome (BAC) , which can contain DNA fragments of about 150 kb. (learner.org)
MPSS provides blanket sequencing of all the cell-free DNA in maternal blood, which "allows us to estimate the relative amount of each chromosome," said Dirk van den Boom, executive vice president of research at Sequenom. (the-scientist.com)

NEW YORK (GenomeWeb News) - Cancer Genetics today said that it and Nikon's Italian subsidiaries have entered into a distribution agreement covering Cancer Genetics' DNA probes intended for fluorescent in situ hybridization. (genomeweb.com)
Here we present Hybridization of Probes to RNA for sequencing (HyPR-seq), a method to sensitively quantify the expression of hundreds of chosen genes in single cells. (nsf.gov)
Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
The degree of homology between target and probe results in stable hybridization. (enzolifesciences.com)
Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)
This review is about "molecular cytogenetics" including 1) the historical perspective of its development from cytogenetics, 2) technical aspects, 3) available probe sets, and 4) variants and applications of the basic fluorescence in situ hybridization (FISH) approach. (frontiersin.org)
The results of MSRF were confirmed by southern hybridization analysis using the aberrantly methylated DNA fragments as the probes. (cdc.gov)
Male fetal cells can be distinguished from female maternal cells by fluorescence in situ hybridization (FISH) with CEP X SpectrumOrange/ CEP Y SpectrumGreen DNA probes. (ugent.be)
Specificity of the product was confirmed with hybridization to a labeled probe. (cdc.gov)
The specificity of the product was confirmed by hybridization to a digoxigenin-labeled probe according to the method described in Boehringer Mannhein's Genius System User's Guide for Filter Hybridization. (cdc.gov)

However, this approach is largely limited to in vitro applications because of poor cell membrane permeability of AdoMet analogs. (uni-goettingen.de)
In practice, double and single stranded DNAs, mRNAs, and other RNAs synthesized in vitro are all used as probes. (enzolifesciences.com)
Although the pathogenic mechanisms in erythema ab igne are poorly understood, one study has shown that moderate heat acts synergistically with ultraviolet radiation to denature DNA in squamous cells in vitro. (medscape.com)
Although researchers have seen the i-motif before and have studied it in detail, it has only been witnessed in vitro -- that is, under artificial conditions in the laboratory, and not inside cells. (sciencedaily.com)
Clone G146-254 has been shown to recognize in vitro translated, recombinant bacterially expressed, and endogenous B cell Fli-1. (bdbiosciences.com)
Here we compared T-cell functions including the number of circulating CD3 + T cells, in vitro responses to mitogens, T-cell receptor (TCR) repertoire, TCR excision circles (TREC) levels, and regulatory T cells (Tregs) enumeration in several immunodeficinecy subtypes, clinically presenting with nonreactive residual cells (MHC-II deficiency) or reactive cells. (hindawi.com)

The highly interdisciplinary project aims to develop cell membrane permeable AdoMet analogs and use them for DNA or RNA modifying enzymes which can position fluorescent probes at the specific sequences. (uni-goettingen.de)
It involves chemical synthesis of the cofactor analogues and mutagenesis of the enzymes acting on nucleic acids combined with selection of the variants which can incorporate fluorescent labels into natural DNA or RNA. (uni-goettingen.de)
Typical biological recognition elements used in biosensors range from DNA probes, antibodies, enzymes, cells and tissues. (msu.edu)

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. (sigmaaldrich.com)

Bucevičius J, Kostiuk G, Gerasimaitė R, Gilat T, Lukinavičius G (2020) Enhancing the biocompatibility of rhodamine fluorescent probes by a neighbouring group effect. (uni-goettingen.de)
To take advantage of this super resolution microscopy, one must be able to select with high specificity the area to be examined using fluorescent probes. (cytoskeleton.com)
In addition, the fluorescent probes must be bright, photostable, exhibit no or little phototoxicity, be excited and emit in the far red spectrum. (cytoskeleton.com)

The goals for advances in flow cytometry are clear: measure as many relevant target molecules per cell as quickly as possible. (nature.com)
Indeed, granule cells from the rostral region of perinatal Gpr56 −/− cerebella show loss of adhesion to extracellular matrix molecules of the pial basement membrane. (jneurosci.org)
Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
Nick translation is efficient for both linear and covalently closed DNA molecules, and labeling reaction are completed in less than an hour. (enzolifesciences.com)
MHC-II molecules drive the development, activation, and homeostasis of CD4 + T-helper cells. (hindawi.com)
Moreover, while the mainstay of the diagnosis of MHC-II deficiency is the absence of constitutive and inducible expression of MHC-II molecules on all cell types, other tests for T-cell function are less informative in such patients. (hindawi.com)

Its technology in combination with Cancer Genetics' DNA-FISH probes "will allow laboratories to perform simultaneous multicolor FISH testing with complex image analysis in academic and clinical settings," Cancer Genetics, which went public last month, said. (genomeweb.com)

Lysate from Jurkat cells was probed with anti-Fli-1 (clone G146-254) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). (bdbiosciences.com)
A 25 μl reaction containing 10 ng HeLa DNA or 5 ng Jurkat total RNA, 1X Master Mix, 1X LAMP primers and fluorescent dye was incubated on a Bio-Rad CFX98 instrument at 65°C for 75 minutes. (neb.com)

Fluorescence and isotope tagging are the principal means for measuring antibody binding to cells in flow cytometry. (nature.com)
Use of DAPI as a DNA stain for flow cytometry was also demonstrated around this time. (wikipedia.org)

These results were confirmed on the identification that are reliable, but faster and more cost-ef- basis of comparisons of the DNA sequences of represen- fective. (cdc.gov)
In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNA sequences. (biogenex.com)
An appropriately labeled probe is hybridized to the exposed target DNA or mRNA sequences in the cells. (biogenex.com)

In the knot structure, C letters on the same strand of DNA bind to each other -- so this is very different from a double helix, where 'letters' on opposite strands recognise each other, and where Cs bind to Gs [guanines]. (sciencedaily.com)

High-intensity femtosecond laser pulses were used to probe damage to aqueous DNA. (laserfocusworld.com)
The experimental technique of generating, in situ , slow electrons and radicals within aqueous media has important implications in different scenarios where the effects of nonionizing radiation need to be probed under physiologically relevant conditions,' says Professor Deepak Mathur, senior scientist at TIFR Mumbai and the lead scientist of the study. (laserfocusworld.com)

Samples were tested for the presence of amplifiable DNA and absence of inhibitors by performing beta globin PCR. (cdc.gov)
To determine the expression of tissue inhibitors of metalloproteinases (TIMP-2) in oral squamous cell carcinoma (OSCC) and the difference in its expression level between positive and negative HPV-16 (human papilloma virus- 16) OSCC patients. (bvsalud.org)

Its optimized structure allows quick labeling of DNA in live & fixed cells with high specificity and very low background. (cytoskeleton.com)
Because of its high specificity (100%) and sensitivity, cell culture is the only test that should be used to establish the presence or absence of infections in cases with legal implications, such as those involving rape or sexual abuse. (medscape.com)

DAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole , is a fluorescent stain that binds strongly to adenine - thymine -rich regions in DNA . (wikipedia.org)

Pinpoint FISH is a fully synthetic FISH technology based on single stranded fluorescently labeled DNA oligonucleotides. (biocat.com)

Here we tracked micronucleated chromosomes with live-cell imaging and show that acentric fragments cluster in close spatial proximity throughout mitosis for asymmetric inheritance by a single daughter cell. (nature.com)
Mechanistically, the CIP2A-TOPBP1 complex prematurely associates with DNA lesions within ruptured micronuclei during interphase, which poises pulverized chromosomes for clustering upon mitotic entry. (nature.com)
Mitotic clustering facilitates the reassembly of acentric fragments into rearranged chromosomes lacking the extensive DNA copy-number losses that are characteristic of canonical chromothripsis. (nature.com)
The main part deals with examples of modern FISH-applications, highlighting unique possibilities of the approach, like the possibility to study individual cells and even individual chromosomes. (frontiersin.org)
They also showed that i-motifs appear in some promoter regions (areas of DNA that control whether genes are switched on or off) and in telomeres, 'end sections' of chromosomes that are important in the aging process. (sciencedaily.com)
Then, known segments of the marked chromosomes (which can contain very small fragments of DNA) are cloned in plasmids . (learner.org)
Approximately 3 to 10 percent of the cell-free DNA in a mother's blood belongs to her baby, and these fetal blueprints are enough to determine if the baby has the wrong number of certain chromosomes-the cause of some inherited diseases, including Down syndrome. (the-scientist.com)
Centromere Enumeration Probes (sub-CEPs) from KromaTiD are available for all human chromosomes as standard products. (biocat.com)
Telomeric Probes (sub-Telos) from KromaTiD are available for the p- and q-arms of all human chromosomes as standard products, other sequenced species are available by request. (biocat.com)

The multicolour probes attach to a certain DNA fragment. (wikipedia.org)
Whether you are performing your first cloning experiment or constructing multi-fragment DNA assemblies, NEB ® has the solution for you. (neb.com)

Bucevičius J, Gilat T, Lukinavičius G (2020) Far-red switching DNA probes for live cell nanoscopy. (uni-goettingen.de)

This assay detects DNA at varying concentrations, providing a qualitative result of either positive, negative, or inconclusive in the identification of Orthopoxvirus infections, other than smallpox (caused by variola virus). (cdc.gov)

• Yeast cells stain blue [Gram positive]. (slideshare.net)
Examine stain for budding cells to confirm that it is a yeast cell and not an artifact. (slideshare.net)
As DAPI can pass through an intact cell membrane , it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore provides a marker for membrane viability. (wikipedia.org)
This led to its use in identifying mitochondrial DNA in ultracentrifugation in 1975, the first recorded use of DAPI as a fluorescent DNA stain. (wikipedia.org)

The researchers looked at areas of the genome with tandem repeats - stretches of 2 to 20 nucleotides, which are the 'building blocks' of DNA, that are repeated two or more times in one spot. (spectrumnews.org)

Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)

Single-cell quantification of RNAs is important for understanding cellular heterogeneity and gene regulation, yet current approaches suffer from low sensitivity for individual transcripts, limiting their utility for many applications. (nsf.gov)
The matrix factorization results in a low-dimensional representation of the transcript count matrix, which imputes gene abundance for both zero and non-zero entries and can be used to cluster cells. (nsf.gov)
To investigate the potential role of Lsm1p in response to UV radiation, we constructed double mutant strains in which LSM1 was deleted in combination with a representative gene from each of three known yeast DNA repair pathways. (bioone.org)
For the gene family, see B-cell CLL/lymphoma . (wikipedia.org)
DNA hypermethylation and loss of expression of the P16 tumor suppressor gene in cadmium transformed BALB/C-3T3 cells. (cdc.gov)
Further studies have shown that the expression of p16 tumor suppressor gene was significantly lower in the transformed cells compared wiht the non-transformed cells. (cdc.gov)
Since DNA hypermethylation is known to result in gene silencing, it appears that the decreased expression p16 gene in the Cd transformed BALB/c-3T3 cells may be due to its hypermethylation. (cdc.gov)
Further, decreased expression of the p16 gene may in part be responsible for the Cd-induced cell transformation and tumorigenesis. (cdc.gov)
This translocation brings the 5' region of the EWS gene into conjunction with the 3' region of the Fli-1 gene encoding the ets-DNA binding domain. (bdbiosciences.com)
Your second year builds on this knowledge and covers areas such as gene regulation, cell biology and metabolism. (kent.ac.uk)

Cell permeabilization induced by higher intensity voltages that cause irreparable cell membrane openings which triggers APOPTOSIS. (bvsalud.org)

DNA can be extracted from acceptable specimen types including, but not limited to, lesion fluid on a dry swab, lesion fluid swab in viral transport media, lesion fluid on a slide, crust, or lesion roof. (cdc.gov)
DNA extract solely from whole blood alone is not a suitable specimen for Orthopoxvirus diagnostic testing as the viremic phase may have already passed at the time of rash onset. (cdc.gov)
Because C trachomatis grows only within columnar cells, obtaining a specimen that contains cells directly from the urethra or cervix, not on pooled vaginal secretions, is important. (medscape.com)

Strong fluorescence when bound to DNA led to the rapid adoption of DAPI for fluorescent staining of DNA for fluorescence microscopy . (wikipedia.org)

A study in Nature suggests epitope editing in donor stem cells prior to bone marrow transplants can stave off toxicity when targeting acute myeloid leukemia with immunotherapy. (genomeweb.com)
Chronic lymphocytic leukemia ( CLL ) is a type of cancer in which the bone marrow makes too many lymphocytes (a type of white blood cell ). (wikipedia.org)
[4] [9] CLL results in the buildup of B cell lymphocytes in the bone marrow, lymph nodes, and blood . (wikipedia.org)
[17] Less commonly, the disease comes to light only after the cancerous cells overwhelm the bone marrow, resulting in low red blood cells, neutrophils, or platelets. (wikipedia.org)
[18] Whereas, with CLL, diseased cells propagate from within the bone marrow, in SLL they propagate from within the lymphatic tissue. (wikipedia.org)

Orthopoxvirus DNA in clinical specimens by real-time PCR. (cdc.gov)
Nuclear localized HER family receptor tyrosine kinases (RTKs) have been observed in primary tumor specimens and cancer cell lines for nearly two decades. (plos.org)
Cell culture is also preferred for rectal specimens because nonculture test results are difficult to interpret in the presence of stool organisms. (medscape.com)
Only 3 benign breast tumour tissues (12.5%) and none of the healthy breast tissue specimens were HPV-DNA-positive. (who.int)
opment and progression including a The specimens were collected dur- Positive cells were counted in 10 possible viral etiology (5) . (who.int)

and cutting-edge microscopes used to examine transport, motility, and reactivity in cells. (mcgill.ca)
A lysosome-targeted fluorescent probe N(CH2)3-BD-PZ is proposed for real-time monitoring of lysosomal kinetic motility. (bvsalud.org)

Inactivation of CIP2A-TOPBP1 caused acentric fragments to disperse throughout the mitotic cytoplasm, stochastically partition into the nucleus of both daughter cells and aberrantly misaccumulate as cytoplasmic DNA. (nature.com)

The term stem cell is also used in reference to any adult cells that are capable of assisting in the restoration of adult tissue via self-renewal. (citizendium.org)
The use of the pluripotent and/or self-renewing qualities of stem cells is believed to have therapeutic benefits for the regeneration of tissue in humans. (citizendium.org)
Such tissue renewal may be accomplished via the use of adult stem cells, or embryonic stem cells, which may be derived from a human embryo in the blastocyst stage. (citizendium.org)
It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. (cytoskeleton.com)
deletions, amplification and translocation in tissue sections or within individual cells. (biogenex.com)
Subsequent stringent washing steps remove any probe that is non-specifically bound to the tissue section. (biogenex.com)
These cells expand in the periphery, causing tissue infiltration and damage due to breakdown of both central (e.g., autoimmune regulator, AIRE protein dysfunction) and peripheral (FOXP3 + deficiency) tolerance mechanisms [ 3 ]. (hindawi.com)
The 4x and 10x objectives can obtain images of cells grown on standard tissue culture well plates. (sheffield.ac.uk)
Cytology is used mainly for diagnosing infant inclusion conjunctivitis and ocular trachoma through the demonstration of intracytoplasmic C trachomatis inclusions in HeLa cells (ie, continuously cultured carcinoma cell line used for tissue cultures). (medscape.com)
C trachomatis grows well in a variety of cell lines (eg, McCoy and HeLa cells) that can be maintained in tissue culture. (medscape.com)
Incubation in tissue culture is 40-72 hours, depending on the cell type and specific biovar. (medscape.com)

For example, Omenn syndrome, a typical case of impaired T-cell differentiation with abnormal self-reactive cells, is invariably characterized by autoimmune features such as generalized scaly exudative erythroderma, enlarged lymphoid tissues, and peripheral expansion of oligoclonal T-cells, in addition to increased susceptibility for severe infections [ 3 , 4 ]. (hindawi.com)
Laser scanning confocal microscopy is a significant advance in the field of optical microscopy, primarily because it permits sample visualization deeper within living and fixed cells, tissues and other samples. (sheffield.ac.uk)
A further 20 blocks from The ISH results were given percentage vanced stages with a likely prevalence samples from normal breast tissues scores based on positive signals and of more aggressive tumour forms were labelled as a control group (i.e. number of cells that gave these signals. (who.int)

During pregnancy, there is a transfer of fetal cells, through the placenta, from the fetus to the blood circulation of women. (ugent.be)
Detection and further characterization of the male fetal cells in women post-partum with an autoimmune disease may result in a better understanding of the pathogenesis of the disease. (ugent.be)
The detected fetal cells will be further characterized using monoclonal antibodies to determine their possible role in auto-immune diseases. (ugent.be)
which may render the genetic mate doses of NDMA is the kidney, but a Transplacental carcinogenesis rial of fetal cells highly accessible to much lower incidence of tumours is stu dies with ENU in nonhuman pri carcinogens. (who.int)

Expresses GFP tagged methyl-CpG recognition motif targeted to mitochondria in mammalian cells. (addgene.org)
Beta globin is common to all mammalian cells and it is reasonable to expect that some human cells will be present in the sample. (cdc.gov)

chromosomal DNA from a Paraguayan Trypanosoma cruzi isolate was extracted and sonicated. (bvsalud.org)

We should think of the human genome as a database of critical information that serves as a tool for exploring the workings of the cell and, ultimately, understanding how a complex living organism functions. (learner.org)

Genetics is more than just DNA. (learner.org)

Single-cell omics provide insight into cellular heterogeneity and function. (nature.com)
KOH dissolves keratin found in cellular material and freeing fungal hyphae and yeast cells. (slideshare.net)

HyPR-seq involves hybridizing DNA probes to RNA, distributing cells into nanoliter droplets, amplifying the probes with PCR, and sequencing the amplicons to quantify the expression of chosen genes. (nsf.gov)

The recurrence is a serious prognostic influence in patients with oral squamous cell carcinoma (OSCC) 1-3 . (bvsalud.org)
2014) 16 reported and compared the histologic, immunohistochemical and clinical features as well as the survival rates of carcinoma arising in patients with PVL (p-scca) and conventional squamous cell carcinoma (c-scca). (bvsalud.org)

Cell permeable fluorescent minor groove-binding probe for DNA. (sigmaaldrich.com)

93X STED-imaged HeLa cell labeled with SPY555-DNA (magenta). (cytoskeleton.com)

We have undertaken studies investigation aberrant DNA methylation resulting in changes in the expression of cancer-related genes as a possible epigenetic mechanism for Cd carcinogenesis. (cdc.gov)
The resulting DNA was analyzed for differential methylation using a PCR-based technique - Methylation Sensitive Restriction Fingerprinting (MSRF). (cdc.gov)

Totipotent cells have the capacity to differentiate to all cell types, including somatic cells, germ cells, and certain cells that exist outside the embryo and are important to fetal development that are termed extraembryonic cells. (citizendium.org)
Chronic myelogenous leukemia (CML), also known as chronic myeloid leukemia, is a myeloproliferative disorder characterized by increased proliferation of the granulocytic cell line without the loss of their capacity to differentiate. (medscape.com)

After the slide was gram stained the slide was scanned under a microscope using low power objective to locate clusters of epithelial cells. (cdc.gov)
Iraq have demonstrated that most patients with benign epithelial mam- 100 lens for counting the positive cells. (who.int)
Cryptosporidia are obligate, intracellular coccidian protozoa that replicate in small-bowel epithelial cells of a vertebrate host. (msdmanuals.com)
After Cryptosporidium oocysts are ingested, they excyst in the gastrointestinal tract and release sporozoites, which parasitize gastrointestinal epithelial cells. (msdmanuals.com)

The DNA samples (which are 25-80 base pairs in length) are then placed on slides to be observed under microscope. (wikipedia.org)

Many isochinoline alkaloids are fluorescent and interact with DNA by intercalation. (muni.cz)
This quantum-mechanical modeling has rationalized the absorption and fluorescence behavior given by minor groove binding and intercalation in the DNA pocket, in term of a reduced structural flexibility and polarization. (wikipedia.org)

When most of us think of DNA, we think of the double helix," says Associate Professor Daniel Christ (Head, Antibody Therapeutics Lab, Garvan) who co-led the research. (sciencedaily.com)

Human embryos fertilized in the ordinary manner and harvested in the blastocyst stage have been used as an extensive source of stem cells for research purposes, and have been shown to possess therapeutic value in laboratory animals. (citizendium.org)

The probe Coa-SH showed high selectivity for the detection of Hg2+ by desulfurization reactions in solution. (bvsalud.org)

The latter includes patients with autoreactive clonal expanded T cell and patients with alloreactive transplacentally maternal T cells. (hindawi.com)
While the autoreactive cells displayed significantly reduced Tregs numbers, the alloreactive transplacentally acquired maternal lymphocytes had high functional Tregs. (hindawi.com)
In some cases, T cells may present, as a result of residual autologous cells or transplacentally acquired maternal lymphocytes [ 1 ]. (hindawi.com)
The origin of the reactive cells in such patients are either thymic release of T-cells that expand at the periphery or transplacentally transfers of maternal T lymphocytes. (hindawi.com)
A distinctive feature of SCID patients, which sometimes can clinically resemble Omenn, [ 6 ] is the presence of alloreactive cells originated from transplacentally maternal T lymphocytes. (hindawi.com)
The maternal placenta, an incomplete bidirectional barrier, allows transfer of maternal cells to occur in healthy neonates. (hindawi.com)
Fetal cell microchimerism is presumed to be associated with some maternal autoimmune diseases such as systemic sclerosis and autoimmune thyroid diseases because of the high incidence in women post-partum and because of the similarities with graft-versus-host disease. (ugent.be)

HyPR-seq achieves high sensitivity for individual transcripts, detects nonpolyadenylated and low-abundance transcripts, and can profile more than 100,000 single cells. (nsf.gov)
Single-cell RNA-sequencing (scRNA-seq) enables high throughput measurement of RNA expression in individual cells. (nsf.gov)
High-bandwidth detection of short DNA in nanopipettes. (imperial.ac.uk)
Scientists in India have shown that damage to DNA can be induced by high-intensity ultrafast laser light. (laserfocusworld.com)
Diagnosis is typically based on blood tests finding high numbers of mature lymphocytes and smudge cells. (wikipedia.org)
Most people are diagnosed as having CLL based on the result of a routine blood test that shows a high white blood cell count, specifically a large increase in the number of circulating lymphocytes . (wikipedia.org)
While all cells are functionally deficient, resulting in high susceptibility to infections, only some of these cells are causing autoimmune symptoms. (hindawi.com)
This is a streamlined and high-throughput way to look into protein functions inside individual cells," said Savas Tay , professor of molecular engineering and senior author of the new work. (eurekalert.org)
On the other hand, the nanotheranostic showed a high loading efficiency of hairpins and achieved the sensitive and selective detection of miR-21 both in living cells and in vivo. (bvsalud.org)
Previous ing the period 2008-11 from major different fields of 100 cells for each studies have demonstrated the pres- hospitals and private laboratories in sample and the average percentage ence of high-risk human papillomavi- the mid-Euphrates governorates of of positive cells within the 10 fields rus (HPV) genotypes 16, 18 and 33 in Iraq including Babylon, Kerrbla, Al- was determined. (who.int)

To accomplish this goal, various intracellular regions of HER3 were fused to the DNA binding domain of the yeast transcription factor Gal4 (Gal4DBD) and tested for their ability to transactivate Gal4 UAS-luciferase. (plos.org)

In recent years, with the advent of fast and cheap genetic sequencing technologies, scientists have turned to single-cell mRNA sequencing to get snapshots of cell's internal states. (eurekalert.org)

Development of most solid tumors is characterized by an increase in secretion and activation of matrix metalloproteinases (MMPs) produced by either the tumor cells or tumor-associated fibroblasts. (bvsalud.org)

A collaborative project between TIFR Mumbai, the Centre for Excellence in Basic Sciences, Mumbai, and Manipal University, the experiments used different incident laser energies and various external focusing conditions to establish that DNA damage occurs in two distinct regimes. (laserfocusworld.com)
The i-motif is a four-stranded 'knot' of DNA," says Associate Professor Marcel Dinger (Head, Kinghorn Centre for Clinical Genomics, Garvan),.who co-led the research with A/Prof Christ. (sciencedaily.com)

With minimal instrumentation (four 96-well plates and a multichannel pipette), cell-specific codes are added to each tagged molecule within cells through sequential rounds of classical split-pool synthesis. (nature.com)
Furthermore, the use of Taq or other thermostable DNA polymerases permits labeling reactions to be performed at higher temperatures via PCR, thereby reducing the incidence of enzyme-mediated point mutations during probe synthesis. (enzolifesciences.com)
PCR is an excellent method for probe synthesis, requiring very small quantities of template material. (enzolifesciences.com)

Mol Cell Biol. (bdbiosciences.com)

Experiments were designed to amplify a DNA target (BRCAb) and a RNA target (HMBS2) by LAMP or RT-LAMP, respectively. (neb.com)

Researchers have recently described new lines of stem cells derived from amniotic fluid [9] . (citizendium.org)
Earlier work carried out by the researchers showed that OH radicals were four times more likely than electrons to produce double-strand breaks in DNA. (laserfocusworld.com)
Our recent advances in image correlation spectroscopic techniques now allow researchers to precisely follow the macromolecular dynamics in living cells. (mcgill.ca)
In a world first, researchers have identified a new DNA structure -- called the i-motif -- inside cells. (sciencedaily.com)
With the new tool, researchers uncovered the location of 'i-motifs' in a range of human cell lines. (sciencedaily.com)

ABSTRACT: Motivation Single-cell RNA sequencing (scRNA-seq) captures whole transcriptome information of individual cells. (nsf.gov)

Anatomy23

Organisms23

Diseases11

Chemicals and Drugs68

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes63

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Humanities1

Information Science4

Health Care7

Double-stranded12

Assays8

Molecular probes4

Detect11

Proteins21

Nuclei2

Polymerase8

Sequence13

Genomic6

Dyes5

Chromosome4

Hybridization10

Vitro6

Enzymes3

Ethidium bromide1

Fluorescent probes3

Molecules6

Multicolor1

Jurkat2

Flow cytometry2

Sequences3

Bind1

Aqueous2

Inhibitors2

Specificity2

Binds1

Single stranded1

Chromosomes9

Fragment2

20201

Concentrations1

Stain4

Nucleotides1

Reagents1

Gene10

Apoptosis1

Specimen3

Fluorescence microscopy1

Bone marrow5

Specimens5

Motility2

Aberrantly1

Tissue11

Tissues3

Fetal cells4

Mammalian cells2

Chromosomal1

Genome1

Genetics1

Cellular2

Involves1

Squamous2

Permeable1

HeLa1

Methylation2

Capacity to differentiate2

Epithelial4

Samples1

Intercalation2

Antibody1

Laboratory1

Selectivity1

Maternal7

High10

Yeast1

MRNA1

Tumor cells1

Centre2

Synthesis3

Biol1

Amplify1

Researchers5

ABSTRACT1