• 2017
  • SMi Group proudly presents the inaugural launch of 3D Cell Culture, an exciting new conference scheduled to take place on the 22nd and 23rd February 2017, London UK. (smi-online.co.uk)
  • 3D Cell Culture 2017 will bring together leading professionals and researchers in the industry to discuss the latest developments and future potential of this technique. (smi-online.co.uk)
  • 3D Cell Culture 2017 will present success stories 3D culture applications, identifying the newest and brightest technologies, and the translation of 3D models into successful clinical and industrial applications. (smi-online.co.uk)
  • Corning Incorporated will showcase its life sciences solutions Nov. 9 and 10 at the 2017 Cell Culture & Bioprocessing Congress in London, U.K. (corning.com)
  • biology
  • Scientific advances in cancer cell biology are changing the paradigm in oncology research. (corning.com)
  • He has over 25 years experience in cell biology with specific interests in cell culture technology, neuroscience and stem cell research. (smi-online.co.uk)
  • a plus) *Clone cell lines *Examine mixture of cell populations *Determine cells by type *Look at cell markers *Cell transfection *Receptor signal transduction *Knowledgeable in primary culture and sterile technique preferred *Computer experience preferred *Molecular biology lab work 3-6 years experience is required. (bio.net)
  • Microfluidic cell culture attempts to develop devices and techniques of culturing, maintaining, analyzing and experimenting in cells in micro scale volumes and facilitate simultaneous manipulation and analysis of cultured cells.The use of integrated microfluidic devices has advanced the fields of quantitative and systems in biology. (wikipedia.org)
  • vitro cell
  • Eric Simon, in a 1988 NIH SBIR grant report, showed that electrospinning could be used to produced nano- and submicron-scale polystyrene and polycarbonate fibrous mats (now known as scaffolds) specifically intended for use as in vitro cell substrates. (wikipedia.org)
  • toxicity
  • This often compromises experiments in basic life science, leads to misleading drug-screening results on efficacy and toxicity, and produces cells that may lack the characteristics needed for developing tissue regeneration therapies. (wikipedia.org)
  • Organotypic liver cell cultures can be used e.g. in drug discovery for testing liver toxicity and metabolism of the novel drug candidates. (wikipedia.org)
  • serum
  • It can be used to distinguish between proteins secreted by cells in culture and serum contaminants. (wikipedia.org)
  • Once a hybridoma colony is established, it will continually grow in culture medium like RPMI-1640 (with antibiotics and fetal bovine serum) and produce antibodies. (wikipedia.org)
  • However, some claim they are not true alternatives because simulations use data from prior animal experiments and cell cultures often require animal derived products, such as serum or cells. (wikipedia.org)
  • Although exact figures are difficult to obtain, some have estimated that one million fetal cows are sacrificed each year to obtain the world's supply of fetal bovine serum, used to grow cultured cells. (wikipedia.org)
  • protocols
  • From product selection guides to application notes to protocols, our online resource library is available anytime you need to facilitate your cell culture applications. (corning.com)
  • cgb at biomail.ucsd.edu (Cindy Gustafson-Brown) wrote: I am interested in generating primary keratinocyte cell cultures and would appreciate any advice, references, protocols, and sources of reagents. (bio.net)
  • Epithelial Cell Culture Protocols, Second Edition provides a cross section of up-to-date culture protocols for the most heavily studied cell systems and featured supporting technologies. (springer.com)
  • Alternatively, the B-cells and myelomas can be made to fuse by chemical protocols, most often using polyethylene glycol. (wikipedia.org)
  • 2018
  • Building on the sell-out success of the launch event, SMi Group is delighted to announce the return of 3D Cell Culture , taking place on 21st and 22nd of February 2018, in London UK. (smi-online.co.uk)
  • We look forward to meeting you at 3D Cell Culture 2018 in Freiburg! (dechema.de)
  • Biotechnology
  • One complication of these blood-derived ingredients is the potential for contamination of the culture with viruses or prions, particularly in medical biotechnology applications. (wikipedia.org)
  • Artificial cells, blood substitutes, and immobilization biotechnology. (wikipedia.org)
  • Corning
  • Optimize your cell culture with Corning vessels, surfaces and media to boost yield, reduce variability, and increase your chances for success - right from the start. (corning.com)
  • Rely on innovative vessels from Corning for quality and consistency in your cell culture applications. (corning.com)
  • Look to Corning for the right surface for every cell-from biological to synthetic-to provide a cell culture environment that meets your research requirements. (corning.com)
  • Drosophila
  • Chapter 7 of Michael Ashburner's 'Drosophila, A Laboratory Handbook' (the big grey one) is called 'tissue culture. (bio.net)
  • There are currently more than 100 insect cell lines available for recombinant protein production with lines derived from Bombyx mori, Mamestra brassicae, Spodoptera frugiperda, Trichoplusia ni, and Drosophila melanogaster being of particular interest. (wikipedia.org)
  • artificial
  • An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. (wikipedia.org)
  • This initiates cell-cell interactions in the absence of any artificial surface or matrix. (wikipedia.org)
  • Insect Cells
  • Insects cell lines are used because post translational modifications of protein are possible in insect cells whereas this mechanism is not present in prokaryotic system The Sf9 is one of the most commonly used cell lines in insect cell culture. (wikipedia.org)
  • Developed by Protein Sciences Corporation, FluBlok is produced with insect cells. (wikipedia.org)
  • physiological
  • For example the direct contact method offers conditions which are most similar to the physiological environment but the cells are susceptible to trauma if the material moves. (wikipedia.org)
  • human
  • When last I interacted with them 2 years ago, their labs were doing primary keratinocyte cultures from human newborn foreskins on a weekly basis. (bio.net)
  • This early use of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types including Human Foreskin Fibroblasts (HFF), transformed Human Carcinoma (HEp-2), and Mink Lung Epithelium (MLE) would adhere to and proliferate upon the fibers. (wikipedia.org)
  • There are similar possibilities for AIDS research: the RCCS can produce human HIV host cells that can be infected and studied. (wikipedia.org)
  • EpiDerm from Mattek and EpiSkin and SkinEthic RHE model are derived from human skin cells which have been cultured to produce a model of human skin. (wikipedia.org)
  • Human epidermal keratinocytes have been cultured to mimic the human epidermis, and are used to measure skin irritation and dermal corrosion. (wikipedia.org)
  • refers
  • Microfluidics refers to a set of technologies for the manipulation for the small fluid volumes within artificially fabricated microsystems.And cell culture refers to the maintenance and growth of cells in a controlled laboratory environment. (wikipedia.org)
  • This refers to the in vitro assessment of material to determine whether it releases toxic chemicals in sufficient quantities to kill cells either directly or indirectly through the inhibition of cell metabolic pathways. (wikipedia.org)
  • rapidly
  • Whilst still in its infancy, 3D Cell Culture is rapidly growing with not only a widespread reach that can be seen across a number of different fields such as 3D bioprinting and microfluidics, but also with incredible potential for industrial application. (smi-online.co.uk)
  • A 3D cell culturing system known as the Bio-Assembler™ uses biocompatible polymer-based reagents to deliver magnetic nanoparticles to individual cells so that an applied magnetic driver can levitate cells off the bottom of the cell culture dish and rapidly bring cells together near the air-liquid interface. (wikipedia.org)
  • viability
  • For the majority of isolated primary cells, they undergo the process of senescence and stop dividing after a certain number of population doublings while generally retaining their viability (described as the Hayflick limit). (wikipedia.org)
  • Appropriate dilutions are then made, depending on cell number in the starting population, as well as the viability and characteristics of the cells being cloned. (wikipedia.org)
  • The 3T3 Neutral Red Uptake (NRU) Phototoxicity Test, approved by the OECD, detects the viability of 3T3 cells after exposure to a chemical in the presence or absence of light. (wikipedia.org)
  • systems
  • Conference on 3D cell cultures as advanced model systems for understanding of diseases, compound testing, successful translation from models to clinical and industrial solutions, and on enabling technologies. (dechema.de)
  • Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the cell growth medium. (wikipedia.org)
  • http://thebigone.stanford.edu/papers/Gomez-Sjoberg%20Anal%20Chem.pdf http://store.elsevier.com/Microfluidic-Cell-Culture-Systems/isbn-9781437734591/ "Microfluidic cell culture" (PDF). (wikipedia.org)
  • Arthropod Cell Culture Systems. (wikipedia.org)
  • mixture
  • A) A magnetic iron oxide nanoparticle assembly known as Nanoshuttle is added and dispersed over cells and the mixture is incubated. (wikipedia.org)
  • hydrogel
  • Several different types of hydrogels from natural and synthetic materials are available for 3D cell culture, including e.g. animal ECM extract hydrogels, protein hydrogels, peptide hydrogels, polymer hydrogels, and wood-based nanocellulose hydrogel. (wikipedia.org)
  • Hydrogel from wood-based nanofibrillated cellulose (NFC) is used as a matrix for 3D cell culture. (wikipedia.org)
  • GrowDex is NFC hydrogel for 3D cell culture commercialized by UPM, Finland. (wikipedia.org)
  • The nanocellulose hydrogel can be completely degraded by cellulase enzyme treatment while retaining the 3D cell structures. (wikipedia.org)
  • embryonic
  • In 1885, Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the principle of tissue culture. (wikipedia.org)
  • Cell-based vaccine is a type of vaccine developed from mammalian cell lines rather than embryonic chicken eggs. (wikipedia.org)
  • developments
  • Our 3-day event, formed of two half day post-conference workshops and a two-day conference will provide useful insights into the latest developments within cell culture. (smi-online.co.uk)
  • Since our first symposium in 2009, the 3D Cell Culture conference series has developed into a 'place to be' for experts active in this field, and will highlight successful applications and newest developments. (dechema.de)
  • growth
  • Professor Przyborski is developing new and innovative ways to manage the growth and function of cultured cells. (smi-online.co.uk)
  • however, only the white cells are capable of growth in culture. (wikipedia.org)
  • Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture. (wikipedia.org)
  • The third dimension of cell growth provides more contact space for mechanical inputs and for cell adhesion, which is necessary for integrin ligation, cell contraction and even intracellular signalling. (wikipedia.org)
  • In 2D, the cells must undergo regular trypsinization in order to provide them with sufficient nutrients for normal cell growth. (wikipedia.org)
  • 3D spheroids have been cultured in a lab setting for up to 302 days while still maintaining healthy, non-cancerous growth. (wikipedia.org)
  • Oxygen, required by cells for growth, is fed into the liquid medium through a porous wall in the chamber. (wikipedia.org)
  • One of the cell populations is fed with growth medium containing normal amino acids. (wikipedia.org)
  • technology
  • Professor Przyborski holds an academic position as Professor in Cell Technology at Durham University. (smi-online.co.uk)
  • Understanding the interplay between critical cell culture parameters and the micro environmental conditions created by microfluidic devices will accelerate the microfluid cell culture technology. (wikipedia.org)
  • Using RCCS technology, researchers could potentially test chemotherapy agents on a patient's own cancer cells from outside the patient's body. (wikipedia.org)
  • microgravity
  • The Rotary Cell Culture System (RCCS) is a device designed to grow three-dimensional cell clusters in microgravity. (wikipedia.org)
  • In the early 1990s, NASA researchers began developing hardware that would let them study the cell tissues of mammals-including humans-in microgravity. (wikipedia.org)
  • The chamber rotates around a horizontal axis, allowing the cells to develop in an environment similar to the free-fall of microgravity. (wikipedia.org)
  • lines
  • Does anybody know the origins of the Schneider cell and Kc cell lines? (bio.net)
  • cell lines (my lit searches have turned up nothing). (bio.net)
  • On page 118, he gives in-text citation of the original papers describing the origins of the cell lines, on pages 133-138 he gives the complete bibliographical references. (bio.net)
  • Adia Technical Services has an immediate opening for a Biologist with the following experience: *Work with mammalian cell culture *Immortalize cell lines (immunochemistry or cancer rsch exp. (bio.net)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • Numerous cell lines are well established as representative of particular cell types. (wikipedia.org)
  • The European Collection of Authenticated Cell Cultures houses and supplies cell lines. (wikipedia.org)
  • laboratory
  • Early studies in the 80's, led by Mina Bissell from the Lawrence Berkeley National Laboratory, highlighted the importance of 3D techniques for creating accurate in vitro culturing models. (wikipedia.org)
  • Viral
  • Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
  • The potential use of cell culture techniques in developing viral vaccines, especially for the Influenza virus, has been widely investigated in recent years as a complementary and alternative platform to the current egg-based strategies. (wikipedia.org)
  • vaccine
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • The United States Food and Drug Administration approved Flucelvax as the first mammalian cell-based Influenza vaccine in the United States on November 20, 2012. (wikipedia.org)
  • Verorab, developed by Sanofi Pasteur, is a mammalian vero cell based rabies vaccine approved by the World Health Organization. (wikipedia.org)
  • antigen
  • A type of white blood cell, the B cell that produces antibodies that bind to the antigen are then harvested from the mouse. (wikipedia.org)