• novel 3D cell culture
  • Network with industry experts, gain insight into how the pharmaceutical industry is utilizing 3D Cell Culture technologies to enhance research and development, discuss the potential for novel in-vitro cell culture models to replace animal models, hear from regulatory bodies, FDA and European Commissions Agency, on how regulatory bodies and researchers can work together, and discover novel 3D cell culture systems, organ-on-chip. (smi-online.co.uk)
  • viability
  • For the majority of isolated primary cells, they undergo the process of senescence and stop dividing after a certain number of population doublings while generally retaining their viability (described as the Hayflick limit). (wikipedia.org)
  • Appropriate dilutions are then made, depending on cell number in the starting population, as well as the viability and characteristics of the cells being cloned. (wikipedia.org)
  • The 3T3 Neutral Red Uptake (NRU) Phototoxicity Test, approved by the OECD, detects the viability of 3T3 cells after exposure to a chemical in the presence or absence of light. (wikipedia.org)
  • vitro cell
  • Eric Simon, in a 1988 NIH SBIR grant report, showed that electrospinning could be used to produced nano- and submicron-scale polystyrene and polycarbonate fibrous mats (now known as scaffolds) specifically intended for use as in vitro cell substrates. (wikipedia.org)
  • serum
  • It can be used to distinguish between proteins secreted by cells in culture and serum contaminants. (wikipedia.org)
  • Once a hybridoma colony is established, it will continually grow in culture medium like RPMI-1640 (with antibiotics and fetal bovine serum) and produce antibodies. (wikipedia.org)
  • However, some claim they are not true alternatives because simulations use data from prior animal experiments and cell cultures often require animal derived products, such as serum or cells. (wikipedia.org)
  • Although exact figures are difficult to obtain, some have estimated that one million fetal cows are sacrificed each year to obtain the world's supply of fetal bovine serum, used to grow cultured cells. (wikipedia.org)
  • Protocols
  • From product selection guides to application notes to protocols, our online resource library is available anytime you need to facilitate your cell culture applications. (corning.com)
  • Several protocols cover the culturing of epithelial cells and their use in treating patients with burns and other skin disorders. (springer.com)
  • Wide-ranging and highly practical, Epithelial Cell Culture Protocols offers both novices and expert investigators alike a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms in general, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells. (springer.com)
  • cgb at biomail.ucsd.edu (Cindy Gustafson-Brown) wrote: I am interested in generating primary keratinocyte cell cultures and would appreciate any advice, references, protocols, and sources of reagents. (bio.net)
  • Alternatively, the B-cells and myelomas can be made to fuse by chemical protocols, most often using polyethylene glycol. (wikipedia.org)
  • human
  • When last I interacted with them 2 years ago, their labs were doing primary keratinocyte cultures from human newborn foreskins on a weekly basis. (bio.net)
  • Researchers globally are realizing the potential of 3D cell culture for various applications, including testing and discovering new drugs to treat cancer, organ-on-chip models to study the human physiology in an organ specific context, and 3D cell printing to produce organ models. (smi-online.co.uk)
  • This early use of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types including Human Foreskin Fibroblasts (HFF), transformed Human Carcinoma (HEp-2), and Mink Lung Epithelium (MLE) would adhere to and proliferate upon the fibers. (wikipedia.org)
  • There are similar possibilities for AIDS research: the RCCS can produce human HIV host cells that can be infected and studied. (wikipedia.org)
  • EpiDerm from Mattek and EpiSkin and SkinEthic RHE model are derived from human skin cells which have been cultured to produce a model of human skin. (wikipedia.org)
  • Human epidermal keratinocytes have been cultured to mimic the human epidermis, and are used to measure skin irritation and dermal corrosion. (wikipedia.org)
  • Drosophila
  • Chapter 7 of Michael Ashburner's 'Drosophila, A Laboratory Handbook' (the big grey one) is called 'tissue culture. (bio.net)
  • There are currently more than 100 insect cell lines available for recombinant protein production with lines derived from Bombyx mori, Mamestra brassicae, Spodoptera frugiperda, Trichoplusia ni, and Drosophila melanogaster being of particular interest. (wikipedia.org)
  • artificial
  • An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. (wikipedia.org)
  • This initiates cell-cell interactions in the absence of any artificial surface or matrix. (wikipedia.org)
  • Artificial cells, blood substitutes, and immobilization biotechnology. (wikipedia.org)
  • 21st
  • Building on the sell-out success of the launch event, SMi Group is delighted to announce the return of 3D Cell Culture , taking place on 21st and 22nd of February 2018, in London UK. (smi-online.co.uk)
  • Insect Cells
  • Insects cell lines are used because post translational modifications of protein are possible in insect cells whereas this mechanism is not present in prokaryotic system The Sf9 is one of the most commonly used cell lines in insect cell culture. (wikipedia.org)
  • Developed by Protein Sciences Corporation, FluBlok is produced with insect cells. (wikipedia.org)
  • refers
  • Microfluidics refers to a set of technologies for the manipulation for the small fluid volumes within artificially fabricated microsystems.And cell culture refers to the maintenance and growth of cells in a controlled laboratory environment. (wikipedia.org)
  • This refers to the in vitro assessment of material to determine whether it releases toxic chemicals in sufficient quantities to kill cells either directly or indirectly through the inhibition of cell metabolic pathways. (wikipedia.org)
  • growth
  • Cell culture is set to see huge increases in growth including the recent advances in techniques such as 3D cell culture. (smi-online.co.uk)
  • Professor Przyborski is developing new and innovative ways to manage the growth and function of cultured cells. (smi-online.co.uk)
  • however, only the white cells are capable of growth in culture. (wikipedia.org)
  • Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the cell growth medium. (wikipedia.org)
  • The third dimension of cell growth provides more contact space for mechanical inputs and for cell adhesion, which is necessary for integrin ligation, cell contraction and even intracellular signalling. (wikipedia.org)
  • In 2D, the cells must undergo regular trypsinization in order to provide them with sufficient nutrients for normal cell growth. (wikipedia.org)
  • 3D spheroids have been cultured in a lab setting for up to 302 days while still maintaining healthy, non-cancerous growth. (wikipedia.org)
  • Oxygen, required by cells for growth, is fed into the liquid medium through a porous wall in the chamber. (wikipedia.org)
  • One of the cell populations is fed with growth medium containing normal amino acids. (wikipedia.org)
  • bacteria
  • Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. (springer.com)
  • hydrogel
  • Several different types of hydrogels from natural and synthetic materials are available for 3D cell culture, including e.g. animal ECM extract hydrogels, protein hydrogels, peptide hydrogels, polymer hydrogels, and wood-based nanocellulose hydrogel. (wikipedia.org)
  • Hydrogel from wood-based nanofibrillated cellulose (NFC) is used as a matrix for 3D cell culture. (wikipedia.org)
  • GrowDex is NFC hydrogel for 3D cell culture commercialized by UPM, Finland. (wikipedia.org)
  • The nanocellulose hydrogel can be completely degraded by cellulase enzyme treatment while retaining the 3D cell structures. (wikipedia.org)
  • microgravity
  • In the early 1990s, NASA researchers began developing hardware that would let them study the cell tissues of mammals-including humans-in microgravity. (wikipedia.org)
  • The chamber rotates around a horizontal axis, allowing the cells to develop in an environment similar to the free-fall of microgravity. (wikipedia.org)
  • applications
  • Lentiviruses are enveloped retroviruses that are becoming major biological tools in cell therapy applications. (corning.com)
  • The 3D Cell Culture conference will highlight successful stories of 3D culture applications as well as the latest technologies and innovations. (corning.com)
  • One complication of these blood-derived ingredients is the potential for contamination of the culture with viruses or prions, particularly in medical biotechnology applications. (wikipedia.org)
  • Applications for the procedure include cloning of parasites, T cells, transgenic cells, and macrophages. (wikipedia.org)
  • Culture of animal cells : a manual of basic technique and specialized applications (6th ed. (wikipedia.org)
  • lines
  • Does anybody know the origins of the Schneider cell and Kc cell lines? (bio.net)
  • cell lines (my lit searches have turned up nothing). (bio.net)
  • On page 118, he gives in-text citation of the original papers describing the origins of the cell lines, on pages 133-138 he gives the complete bibliographical references. (bio.net)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • Numerous cell lines are well established as representative of particular cell types. (wikipedia.org)
  • The European Collection of Authenticated Cell Cultures houses and supplies cell lines. (wikipedia.org)
  • laboratory
  • Early studies in the 80's, led by Mina Bissell from the Lawrence Berkeley National Laboratory, highlighted the importance of 3D techniques for creating accurate in vitro culturing models. (wikipedia.org)
  • Viral
  • Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
  • The potential use of cell culture techniques in developing viral vaccines, especially for the Influenza virus, has been widely investigated in recent years as a complementary and alternative platform to the current egg-based strategies. (wikipedia.org)
  • interaction
  • The onset of cell-cell interaction takes place as soon as cells levitate, and 3D structures start to form. (wikipedia.org)
  • SILAC has emerged as a very powerful method to study cell signaling, post translation modifications such as phosphorylation, protein-protein interaction and regulation of gene expression. (wikipedia.org)
  • vaccine
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • The vaccine was produced by Novartis through culturing of the Madin-Darby canine kidney (MDCK) cell line. (wikipedia.org)
  • Verorab, developed by Sanofi Pasteur, is a mammalian vero cell based rabies vaccine approved by the World Health Organization. (wikipedia.org)
  • Doroshenko A, Halperin S. Trivalent MDCK cell culture-derived influenza vaccine Optaflu (Novartis Vaccines). (wikipedia.org)
  • physiological
  • For example the direct contact method offers conditions which are most similar to the physiological environment but the cells are susceptible to trauma if the material moves. (wikipedia.org)
  • proteins
  • When the cells are growing in this medium, they incorporate the heavy arginine into all of their proteins. (wikipedia.org)
  • The trick is that the proteins from both cell populations can be combined and analyzed together by mass spectrometry. (wikipedia.org)