Cells rats sprague dawley. Medical search. Frequent questions

Methods: MRMT-1 breast cancer cells were injected into the proximal segment of tibia in female Sprague-Dawley rats. (amrita.edu)
Female Sprague-Dawley rats implanted with bone cancer cells develop mechanical and thermal hyperalgesia but antagonizing GM-CSF in these animals significantly reduced such hypersensitivity. (iasp-pain.org)
Sixty one plants reputed to have anti-fertility activity were tested on female Sprague-Dawley rats, in order to isolate active compounds with a potential for use in human fertility regulation. (who.int)

The genotoxic effects of benzene (71432) on bone marrow and spleen lymphocytes were investigated in rats using micronucleus and sister chromatid exchange assay systems. (cdc.gov)
Hydrodesulfurised kerosine was tested in the sister chromatid exchange assay using Chinese hamster ovary cells (API, 1988a). (europa.eu)
The assay was conducted with Aroclor-induced rat liver S-9 activation system. (europa.eu)
Under the conditions of the study, hydrodesulfurised kerosine is considered to be negative in the sister chromatid exchange assay with Chinese hamster ovary cells. (europa.eu)
The viability of lung epithelial BEAS-2B cells, intracellular production of reactive oxygen species, and the levels of pro-inflammatory cytokines were examined by cell counting kit-8 assays, 2',7'-dichlorofluorescein diacetate (DCF-DA) staining, and enzyme-linked immunosorbent assay, respectively. (bvsalud.org)

In a study by the American Petroleum Institute (1977) (Klimisch score = 1), strains of S. typhimurium (TA-1535, TA-1537, TA-1538, TA-98 and TA-100) and S. cerevisiae (D4) were exposed to kerosine in DMSO with or without metabolic activation by Aroclor 1254-induced rat liver microsomes at dose concentrations from 0.001 to 5.0 μL/plate. (europa.eu)
In a study by the American Petroleum Institute (API, 1984b), cultures of mouse lymphoma cells were exposed to hydrodesulfurised kerosine with or without metabolic activation by Aroclor 1254-induced rat liver S9 fraction. (europa.eu)

Nerve cells (also known as neurons) in the brain release ATP when activated, and use this molecule to send signals to other active neurons or other cells in the brain. (elifesciences.org)
The voltage gated Na channels Nav1.7, Nav1.8 and Nav1.9 were found to be selectively up-regulated in rat DRG neurons treated with GM-CSF, which resulted in enhanced excitability. (iasp-pain.org)
Accordingly, targeted knocking down of either Nav1.7-1.9 or Jak2/Stat3 in DRG neurons in vivo alleviated the hyperalgesia in male Sprague-Dawley rats. (iasp-pain.org)
We used single neuron recordings and controlled whisker deflections to examine responses of thalamocortical neurons to sensory stimulation in rat survivors of 9 min of asphyxial cardiac arrest incurred on post-natal day 17. (cdc.gov)

There has been much debate regarding the proper term for this lesion (metaplasia vs. hyperplasia), but since goblet cells are not normally present in the epithelium of the smaller airways and are not normally visible in the upper airways, it should be diagnosed as goblet cell metaplasia in NTP studies. (nih.gov)
Goblet cell metaplasia may occur in association with infectious agents or exposure to inhaled irritants in both rats and mice. (nih.gov)
The preferred terminology for this lesion is Lung - Metaplasia, Goblet cell. (nih.gov)
Lung, Bronchiole - Metaplasia, Goblet cell from a male Sprague-Dawley rat in an acute study. (nih.gov)

1-2% of the epithelial cells of the mainstem bronchi, primary bronchi, and lobar bronchi (up to approximately the sixth-generation airway) are goblet cells. (nih.gov)
1992. Epithelial cells of the trachea and bronchi. (nih.gov)
1992. Epithelial cells of bronchioles. (nih.gov)
Bile acid treatment also increased the expression of KLF5, CDX2, MUC2 and villin in esophageal epithelial cells in a time-dependent manner. (jcancer.org)
Moreover, siRNA-mediated knockdown of KLF5 blocked the expression of CDX2, MUC2 and villin, but transfection of a KLF5 expression vector into esophageal epithelial cells promoted their transdifferentiation into columnar-like cells, as demonstrated by increased expression of the intestinal markers CDX2, MUC2 and villin. (jcancer.org)
During regeneration, type 2 alveolar epithelial cells (AEC2s) assume a transitional state that upregulates multiple keratins and ultimately differentiate into AEC1s. (bvsalud.org)

C, The SDR rat has slightly less circulating NK cells, unlike what is observed in the spleen. (figshare.com)
The cytogenetic effects of benzene on rat bone marrow and spleen cells. (cdc.gov)
In the group treated and followed for 24 hours, the numbers of micronuclei in spleen cells of rats exposed to 220 and 440mg/kg were not significantly different from controls. (cdc.gov)
No effect on the nuclear division index of spleen cells was noted at 24 hours nor was there a significant decrease in the replicative index of bone marrow or spleen cells. (cdc.gov)

Thirteen-week inhalation toxicity of p -dichlorobenzene in mice and rats. (cdc.gov)
Carcinogenicity and chronic toxicity in mice and rats exposed by inhalation to para -dichlorobenzene for two years. (cdc.gov)
Effect of Zingiber zerumbet essential oils and zerumbone inhalation on body weight of sprague dawley rat. (scialert.net)
Thus, based on dosimetric relationships, determinants of airway injury sites following inhalation extrapulmonary airway injury in the rat may be predictive of intrapulmonary airway injury in humans. (cdc.gov)

Rats and mice have very low numbers of goblet (mucous) cells in their lungs. (nih.gov)
We report here that BTA-EG 4 decreases Aβ levels, alters cell surface expression of amyloid precursor protein (APP), and improves memory in wild-type mice. (jneurosci.org)
In a study using testes cells from mice, they confirmed that pomegranate extract raised testosterone production. (lifeextension.com)
The National Toxicology Program (NTP) , headquartered at NIEHS, has conducted toxicology studies in rats and mice to help clarify potential health hazards, including cancer risk, from exposure to radio frequency radiation (RFR) like that used in 2G and 3G cell phones. (nih.gov)
NTP conducted toxicology studies in rats and mice to help clarify potential health hazards, including cancer risk, from exposure to RFR used in 2G and 3G cell phones. (nih.gov)
in mice, most transitional cells and fibrosis resolved, whereas in human IPF, transitional AECs evolved into an aberrant basaloid state that persisted with progressive fibrosis. (bvsalud.org)
Sirtuin 6 (SIRT6) has a critical role in cutaneous Squamous Cell Carcinoma (cSCC): SIRT6 silencing in skin SCC cells has pro-differentiating effects and SIRT6 deletion abrogated DMBA-TPA-induced skin tumorigenesis in mice. (bvsalud.org)

J Cell Biol (2006) 173 (5): 733-741. (rupress.org)

Ninety four adult (150-250 g) male Sprague-Dawley rats (obtained from Zivic-Miller Laboratories, Allison Park, Pa. (erowid.org)
Twenty-four male rat DCD donor livers were randomly and averagely divided into normal, static cold storage (SCS), NMP, and NMP combined with BMMSCs groups. (medscimonit.com)
Male rats were exposed to 3D-printer emissions (592g particulate/m(3) air) or filtered air for 4h/day (d), 4days/week and total exposures lengths were 1, 4, 8, 15 or 30days. (cdc.gov)
Male and female rats were exposed to WBV at the resonant frequency of the torso (31.5Hz, 0.3 g amplitude) for 4hr/day for 10days. (cdc.gov)

Our findings revealed that RSV protected against ischemia-induced mouse heart injury in vivo and hypoxia-induced NRCM injury in vitro via regulating Sirt1/p53-mediated cell senescence and inhibiting NLRP3-mediated inflammasome activation. (hindawi.com)
Although MSC EVs show great promise for regenerative medicine applications, insights into their uptake mechanisms by different target cells and the ability to control MSC EV properties for defined function in vivo have remained elusive knowledge gaps. (frontiersin.org)

Apoptotic oligodendrocytes, changes in the oligodendrocyte progenitor cell (OPC) population and loss of myelin were evaluated at 2, 7 and 21 days following TBI. (lu.se)
Erythropoietin is a glycoprotein hormone that stimulates red blood cell (RBC) production in bone marrow via binding to the cell-surface receptor on hematopoietic progenitor cells, and it has been widely used for treating anemia [ 13 ]. (hindawi.com)
The goal of this protocol is to test the ability of progenitor cells derived from human perivascular adipose tissue to differentiate into multiple cell lineages. (jove.com)
Adipose progenitor cells vary according to their anatomical location. (jove.com)
The pellet is the stromal vascular fraction containing endothelial cells, immune cells, blood cells and progenitor cells. (jove.com)

In this manuscript, we report the in vitro mutagenicity of waterpipe smoke condensate (WSC), the alteration in cellular parameters of lung alveolar cells in response to WSC exposure and discuss the implication of cellular responses in the pathophysiology of chronic obstructive pulmonary disease (COPD). (nih.gov)
Physically, the new exposure system was developed by adding a loading device to an existing rat-tail model. (cdc.gov)
An analytical model of the rat-tail exposure system was proposed and used to formulate the methods for quantifying the biodynamic responses. (cdc.gov)
WBV exposure resulted in a significant reduction in number of developing follicles, and decrease in circulating estradiol concentrations, ovarian luteinizing hormone receptor protein levels, and marked changes in transcript levels for several factors involved in follicular development, cell cycle, and steroidogenesis. (cdc.gov)

Wistar rats) [155], failed to influence food intake during a 1 h ad libitum feeding period, moderate to high doses of CBD (4.4 mg·kg −1 , i.p. [58]. (researchgate.net)

The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. (hindawi.com)
To improve the quality of liver grafts from extended-criteria donors donated after circulatory death (DCD), this study explored whether bone marrow mesenchymal stem cells (BMMSCs) combined with normothermic machine perfusion (NMP) have protective effects on DCD donor livers and the effects of ferroptosis in this procedure. (medscimonit.com)

Hydrogen peroxide was used to induce an oxidative stress model of hepatocyte IAR-20 cells to evaluate the protective effects of BMMSCs in vitro. (medscimonit.com)
In males, WBV resulted in a significant reduction in spermatids and circulating prolactin levels, elevation in number of males having higher circulating testosterone concentrations, and marked alterations in levels of transcripts associated with oxidative stress, inflammation, and factors involved in regulating the cell cycle. (cdc.gov)
Mn, serum Mn, urine Mn, Centromere-positive micronuclei in periphery blood lymphocytes, DNA protein-crosslinks in peripheral white blood cells, oxidative stress as urinary 8OHdG, and long-term oxidative damage measured as erythrocytic superoxide dismutase (SOD) activity (measure of systemic oxidative stress) and serum malondialdehyde (MDA) (product of lipid peroxidation and reflect tissue injuries). (who.int)

In the 1980's, Arnold Caplan and his colleagues published an isolation method of fibroblast-like stromal cells from bone marrow and first identified them as mesenchymal stem cells (MSCs) because of their multilineage differentiation potential ( Caplan, 1991 ). (frontiersin.org)
This study was therefore aimed at establishing the dose-response effect of EPO stimulation on rat BMSCs and investigating the effects of mechanical stretch combined with EPO on the proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
The proliferation and osteogenic differentiation of rat BMSCs were examined and compared using EPO with different concentrations. (hindawi.com)
Notably, it promotes the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) [ 15 - 17 ]. (hindawi.com)
Differentiation was compared to mesenchymal stem cells derived from human bone marrow, which is known to differentiate into adipocyte, osteocyte, and chondrocyte lineages. (jove.com)
Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)

SD Rat Mesenchymal Stem Cells with RFP have a finite lifespan in vitro . (cyagen.com)
Although mesenchymal stem cells (MSCs) have been shown as a novel approach in tissue regeneration, the therapeutic potential of MSCs mediated by the interaction between MSC-derived paracrine mediators and Mφs remains elusive. (frontiersin.org)
The Immortalized Rat Metanephric Mesenchymal Precursor Cells (KMM) is suitable for KSHV infection related studies, such as the growth deregulation mechanisms, as it is able to cause tumor formation. (topsan.org)
Mesenchymal stem cells (MSCs) are multipotent cells with regenerative and immunomodulatory properties. (frontiersin.org)
Human mesenchymal stem cells (HMSCs) are multipotent somatic stem cells that can be isolated from a variety of tissues such as the bone marrow, adipose tissue, and dental pulp. (frontiersin.org)

GO-AgNPs induced significant cytotoxicity by the loss of cell viability, production of reactive oxygen species (ROS), cell cycle arrest, increasing leakage of lactate dehydrogenase (LDH) and level of Malondialdehyde (MDA), increasing expression of pro-apoptotic genes and decreasing expression of anti-apoptotic genes. (researchsquare.com)

BMMSCs showed significant protective effects on the ultrastructure of DCD donor livers and ROS-induced injury to IAR-20 cells under electron microscopy. (medscimonit.com)
BMMSCs also significantly improved the expression level of microtubule-associated protein 1 light chain 3 (LC3)-II in both DCD donor livers and ROS-induced injured IAR-20 cells, including upregulating the expression of ferritin. (medscimonit.com)

Among its diverse biological actions, the vasoactive peptide bradykinin (BK) induces the transcription factor AP-1 and proliferation of mesangial cells (S. S. El-Dahr, S. Dipp, I. V. Yosipiv, and W. H. Baricos. (nih.gov)

In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. (nih.gov)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)

Furthermore, the effect on actin polymerization in fibroblast cell lines was investigated. (nature.com)
Taken together, our study demonstrated that GO-AgNPs potentially induce oxidative damage to DNA, which result in toxicity and cell apoptosis in caprine fetal fibroblast cell due to an increased generation of ROS. (researchsquare.com)

Key in vitro gene mutation studies in mammalian cells were identified. (europa.eu)

Ma et al ( 15 ) revealed that compression activated autophagy in NP cells and that compression-induced autophagy was closely associated with intracellular reactive oxygen species production. (spandidos-publications.com)
A number of standard tests, with and without metabolic activation by inclusion of rat liver S9, were conducted on straight-run kerosines, deodorised kerosine and Jet A. A rather wide variety of tester strains, both of prokaryotic species ( Salmonella typhimurium ) and eukaryotic species ( Saccharomyces cerevisiae ), were used, and also various solvents were applied to dissolve the test product. (europa.eu)
Experimental results revealed that ephedrine treatment rescued the repressive impact of BLM on BEAS-2B cell viability, and ephedrine inhibited BLM-induced overproduction of reactive oxygen species and inflammatory response in BEAS-2B cells. (bvsalud.org)

an open lung biopsy was performed, which revealed constrictive bronchiolitis (the histopathologic correlate of obliterative bronchiolitis) with both narrowed and obliterated airways with surrounding fibrous tissue and a variable mixed chronic inflammatory cell infiltrate. (cdc.gov)

In this study, we aim to understand how tumor-induced primary and distal pain sensitivities are affected by bupivacaine-induced block of bone nerve endings in a rat model of CIBP. (amrita.edu)
On the other hand, SIRT6 acts as tumor suppressor in SCC by enhancing glycolysis in tumor propagating cells. (bvsalud.org)

Subsequently, in collaborations with the County of Los Angeles Department of Health (Los Angeles, CA, USA) Vector Management Program, we succeeded in transmitting to laboratory rats 2 strains of HEV from rats from Los Angeles but were again unable to obtain genomic sequence ( 14 ). (cdc.gov)

Mol Cell Biochem. (oregonstate.edu)
J Cell Biochem 72:94-102. (cdc.gov)

By numerous specific protein-protein interactions, SHANKs are either directly or indirectly linked to other structural proteins, cell adhesion molecules, receptors, ion channels and to actin-interacting proteins at the postsynaptic density. (nature.com)
This molecule carries energy all through the body, and most cells and proteins require ATP to perform their various roles. (elifesciences.org)

After one hour, no visible tissue pieces should be present and the solution will appear as a cloudy cell suspension. (jove.com)

RSV inhibited the expression of senescence markers (p53, p16, and p19), inflammasome markers (NLRP3 and Cas1 p20), and nuclear translocation of NF- κ B, hence alleviating infarction area, fibrosis, and cell apoptosis. (hindawi.com)

A, The wild-type rat has a significant population of circulating CD4+, CD8+, and CD4/CD8 double positive T cell populations compared to the SDR rat. (figshare.com)

Derived from the bone marrow tissues of SD rats and have been transfected with a lentiviral construct encoding a RFP expression motif. (cyagen.com)

The tetra(ethylene glycol) derivative of benzothiazole aniline, BTA-EG 4 , is a novel amyloid-binding small molecule that can penetrate the blood-brain barrier and protect cells from Aβ-induced toxicity. (jneurosci.org)

In a study by API (1977) (Klimisch score = 1), mouse lymphoma L5178Y cells were exposed to straight-run kerosine in acetone vehicle at concentrations ranging from 0.04 to 0.065 μL/mL (with metabolic activation) or 0.006 to 0.13 μL/mL (without activation). (europa.eu)

In the present study, we examined the role of protein tyrosine phosphorylation and the mitogen-activated protein kinases, ERK1/2,in mediating BK-induced AP-1 and DNA replication in cultured rat mesangial cells. (nih.gov)
A multigeneration study to ascertain the toxicological effects of Great Lakes salmon fed to rats: Study overview and design. (cdc.gov)
The present study attempted to evaluate serotonin antagonism on the ypramidal cells of the hippocampus (HPC) which receive serotonin containing afferents from the raphe nuclei. (erowid.org)
This procedure enhanced detection of inhibitory responses, and protected against pre-selection of spontaneously rapid-firing cells for study. (erowid.org)
The test chemical did not induce chromosome aberrations in the mammalian cell line in the presence and absence of S9 metabolic activation system and hence it is not mutagenic in the chromosome aberration study performed. (europa.eu)
In a study on rats, this pomegranate - cacao combination boosted total testosterone levels by over 72% after just six weeks. (lifeextension.com)
Overall, the results presented in this study show a common mechanism of endocytosis for MSC EVs across different cell types and the feasibility to generate functionally enhanced EVs by modifications to parental MSCs. (frontiersin.org)
The objective of this study was to develop a new rat-tail vibration model to investigate the combined effects of vibration and contact pressure and to identify their relationships with the biodynamic responses. (cdc.gov)

2,3,7,8-Tetrachlorodibenzo- p -dioxin as an antiestrogen: Effect on rat uterine peroxidase activity. (cdc.gov)
In this method the isotonic contractions of the isolated uterine horn of the oestrogenised rat were observed in the presence of various plant extracts. (who.int)

Human whole blood was stained with either PE Rat IgG2a, κ Isotype Control (Cat. (bdbiosciences.com)
dashed line histogram) or PE Rat Anti-Human CD49f (Cat. (bdbiosciences.com)
The role of rats in human hepatitis E virus (HEV) infections remains controversial. (cdc.gov)
Rat HEV was not transmissible to rhesus monkeys, suggesting that it is not a source of human infection. (cdc.gov)
Thus, it is important to understand how this rat virus is related to human infections. (cdc.gov)
Rats are particularly interesting as a potential source of human infections because although they are not a human food, they have a high seroprevalence of antibodies against HEV ( 12 , 13 ) and they are ubiquitous and in close contact with humans everywhere. (cdc.gov)
Finally, IKKβ knockdown was used to further confirm the effect of the NF‑κB signal on human NP cells autophagy, and the data showed that IKKβ knockdown upregulated the autophagy of NP cells during inflammatory conditions. (spandidos-publications.com)
Human esophageal squamous epithelial (HET-1A) cells were treated with bile acid and used in transfection experiments. (jcancer.org)
Epithelial tissue from both the rat BE model and human BE patients strongly expressed KLF5, CDX2, MUC2, and villin. (jcancer.org)
penetrate to the intrapulmonary airways to a greater degree in the Regional deposition patterns of inspired materials are critical human than in the rat. (cdc.gov)

A critical role of autophagy was identified in rat nucleus pulposus (NP) cells: Inhibition of autophagy suppresses, while activation of autophagy enhances, the catabolic effect of cytokines. (spandidos-publications.com)

Glucose rapidly increased DAG content and PKC enzyme activity in microsomes, but not in plasma membranes or other membranes, during a 30-min treatment of rat adipocytes. (jci.org)

In addition, Shen et al ( 14 ) reported that the autophagy of rat AF cells was induced by serum deprivation in vitro and that IL-1β upregulated serum deprivation-induced autophagy in a dose-dependent manner. (spandidos-publications.com)

We also assessed the effect of WSC on the expression of genes involved in cell cycle arrest and inflammation. (nih.gov)
The effects on dopamine (DA) release and dopamine transporter (DAT) gene expression were assayed in hypothalamic HypoE22 cells. (researchgate.net)
In the same experimental system, IL-10 and BDNF gene expressions increased, and in HypoE22 cells, the extract decreased the extracellular dopamine level and increased the DAT gene expression due to the direct interaction of parthenolide with the DAT. (researchgate.net)

Computer simulations suggest that on Titan conditions exist such that the compound could form structures similar to cell membranes and vesicles on Earth, called azotosomes. (wikipedia.org)

Benzene caused a significantly higher sister chromatid exchange (SCE) frequency in bone marrow cells compared to controls. (cdc.gov)

However, whether and how transitional cells cause fibrosis, whether keratins regulate transitional cell accumulation and fibrosis, and why transitional AECs and fibrosis resolve in mouse models but accumulate in IPF are unclear. (bvsalud.org)

If there is dry ice left in the package, store cells in liquid nitrogen immediately upon arrival. (cyagen.com)

In IVDs, autophagy is also present and associated with the increased pathological process of IVD degeneration in rats. (spandidos-publications.com)

However, an HEV strain recently isolated from rats was unique and only distantly related to known strains ( 11 ). (cdc.gov)
Unlike the previous model, the vibration strain and stress of the rat tail does not depend primarily on the vibration response of the tail itself but on that of the loading device. (cdc.gov)

In addition, neonatal rat cardiomyocytes (NRCMs), cardiac fibroblasts (CFs), and macrophages were isolated for in vitro experiments. (hindawi.com)

However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. (nih.gov)

As men age, their testosterone tends to bind to a blood protein rendering the testosterone unavailable to cells throughout the body. (lifeextension.com)

Anatomy17

Organisms12

Diseases10

Chemicals and Drugs33

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Psychiatry and Psychology4

Phenomena and Processes28

Disciplines and Occupations1

Technology, Industry, Agriculture1

Health Care3

Female Sprague-Dawley rats3

Assay5

1254-induced2

Neurons4

Metaplasia4

Epithelial Cells6

Spleen4

Inhalation4

Mice7

Biol1

Male4

Vivo2

Progenitor5

Exposure4

Wistar1

Bone marrow mesenchymal stem2

Oxidative stress3

Differentiation6

Mesenchymal5

Production of reactive oxygen1

Livers2

Induces1

Proliferation2

Fibroblast cell2

Mammalian cells1

Species3

Lung1

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Subsequently1

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Proteins2

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