• Endometrium-derived epithelial cells (Ishikawa) showed no response to the natural compound by using a cell viability assay (MTT). (nutrimedical.com)
  • Cell viability was examined by MTT assay and lipid peroxidation was assayed by thiobarbituric acid (TBA) reaction. (spandidos-publications.com)
  • Lipid peroxidation assay protocol summary: - add TBA reply to samples and necessities, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min - swap to wells of microplate - analyze with microplate reader For higher sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet sooner than analysis. (accenx.com)
  • For example, temozolomide (TMZ), of use for glioblastoma (GBM) treatment, appears as capable of inducing autophagy partially inhibiting cancer cell proliferation. (nature.com)
  • miRNAs have been implicated as transcriptional regulators in a wide range of biological processes determining cell fate, stress response, proliferation, or death [ 5 ]. (hindawi.com)
  • For apoptotic inducing activity, human osteogenic sarcoma (HOS) cell proliferation, chromosomal DNA degradation and changes in apoptosis-related protein levels were measured. (nutrimedical.com)
  • Ellagic acid significantly reduced HOS cell proliferation, and induced apoptosis evidenced by chromosomal DNA degradation and apoptotic body appearance. (nutrimedical.com)
  • It is evidenced by increased cell proliferation, anchorage-independent growth, migration and invasion. (cdc.gov)
  • Most cancer cells undergo metabolic reprogramming that support their survival and proliferation. (degruyter.com)
  • Therefore, this study proves that Rh4 inhibits cancer cell proliferation by activating the ROS/p53 signaling pathway and activating autophagy to induce ferroptosis, which provides necessary scientific evidence of the great anticancer potential of Rh4. (hindawi.com)
  • Living organisms have different molecules that speed up termination by neutralizing free radicals and, therefore, protecting the cell membrane. (wikipedia.org)
  • full citation needed] If not terminated fast enough, there will be damage to the cell membrane, which consists mainly of lipids. (wikipedia.org)
  • Native honey (1% w/v pH 7.4, 10(6) cells) showed strong quenching activity against lipophilic cumoxyl and cumoperoxyl radicals, with significant suppression/prevention of cell damage, complete inhibition of cell membrane oxidation, of intracellular ROS production and recovery of intracellular GSH. (researchgate.net)
  • Each of those cells is surrounded by a membrane that is bean shaped. (vitanetonline.com)
  • Plasma membrane permeabilization following cell death : many ways to dye! (ugent.be)
  • The application of ascorbic acid reduced cell membrane damage and increased catalase activity in water-stressed trees of both cultivars. (frontiersin.org)
  • The influx of calcium activates a series of destructive enzymes, including phospholipases that go on to damage the phospholipid cell membrane and proteases thereby destroying proteins. (scireproject.com)
  • [ 3 ] This protein controls zinc uptake across the plasma membrane of various cell types, including the intestine. (medscape.com)
  • After 24 h of exposure to different doses (4-32 μg/mL) of BP-QDs, intracellular lipid peroxidation and iron overload occurred in Beas-2B cells. (medworm.com)
  • Results showed that in cultured human-derived retinal pigment epithelial ARPE-19 cells , the extract of Chlorella zofingiensis and its nutritional ingredient astaxanthin exhibited significant inhibitory effects on the formation of endogenous N ε -carboxymethyllysine (CML), a key AGE representative, through the suppression of intracellular oxidative stress. (rsc.org)
  • [ 4 ] Protein hZIP4 transports zinc ions from the cell exterior or lumen of intracellular organelles into the cytoplasm, where it is available to other newly synthesized proteins. (medscape.com)
  • The effect of 200 and 400 mg/kg body weight of 80% ethanolic extract of the fresh leaves of Lawsonia inermis were examined on drug metabolizing phase-I and phase-II enzymes, antioxidant enzymes, glutathione content, lactate dehydrogenase and lipid peroxidation in the liver of 7 weeks old Swiss albino mice. (nih.gov)
  • Activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) were measured in ellagic acid-treated V79-4 cells. (nutrimedical.com)
  • S. Slathia, A. Sharma and S. Choudhary, "Influence of Exogenously Applied Epibrassinolide and Putrescine on Protein Content, Antioxidant Enzymes and Lipid Peroxidation in Lycopersicon esculentum under Salinity Stress," American Journal of Plant Sciences , Vol. 3 No. 6, 2012, pp. 714-720. (scirp.org)
  • DNA damage assessment and cell cycle analysis revealed differences in the modes of cell death between asbestos and respective CF. There was an increase in chemokines, but not pro-inflammatory cytokines after all EMP treatments. (cdc.gov)
  • Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)
  • Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)
  • The presence of immune cells can initially be advantageous because they remove cellular debris that resulted from the original injury in an effort to make room for new neurons to grow. (scireproject.com)
  • Ferroptosis is an oxidative, iron-dependent form of cell death. (medworm.com)
  • In summary, findings in the present study confirmed that BP-QDs induce ferroptosis via increasing lipid peroxidation and iron accumulation in vitro and in vivo.PMID:3748. (medworm.com)
  • Death of GSCs was apparently due to the iron dependent form of programmed cell death characterized by the accumulation of lipid peroxides called ferroptosis. (nature.com)
  • Ferroptosis is a novel type of regulated cell death caused by iron-dependent lipid peroxidation [ 26 ]. (hindawi.com)
  • In this study, we examined CEPA-induced gene expression in DLD-1 colorectal adenocarcinoma human cells carrying a mutant p53, in order to understand the details of CEPA-induced apoptosis via lipid peroxidation. (elsevierpure.com)
  • In murine cardiomyocytes miR-21 was found to protect from hypoxia/reoxygenation (H/R)-induced cell apoptosis via regulation of its target gene PDCD4 [ 13 ]. (hindawi.com)
  • Additionally, a significant depletion of antioxidants, accumulation of oxidatively modified proteins and an increase in lipid peroxidation products were found in the cells exposed to CNC. (cdc.gov)
  • The principal histopathological features are necrosis of fat cells, extensive macrophage infiltration leading to the formation of granulomatous structures, and the accumulation of lipopigment by lipid peroxidation. (int-res.com)
  • Malondialdehyde (MDA) is the most widely used index of lipid peroxidation 13 and is easily measured by the thiobarbituric acid reaction. (bmj.com)
  • The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. (researchgate.net)
  • Experiments with endothelial cells fortified with the isolated fraction from native honey enriched in antioxidants, exposed to peroxyl radicals from 1,1-diphenyl-2-picrylhydrazyl (AAPH, 10 mM) and to hydrogen peroxide (H2O2, 50-100 microM), indicated that phenolic acids and flavonoids were the main causes of the protective effect. (researchgate.net)
  • Recent studies have demonstrated that antioxidants present in natural products protect cells by reducing H 2 O 2 -induced oxidative stress in vitro and in vivo ( 5 - 7 ). (spandidos-publications.com)
  • M. Valko, D. Leibfritz, J. Moncol T. D. Mark, M. C. Mi- lan and J. Telser, "Free Radicals and Antioxidants in Normal Physiological Functions and Human Disease," The International Journal of Biochemistry and Cell Biol- ogy, Vol. 39, No. 1, 2007, pp. 44-84. (scirp.org)
  • Since cancer stem cells have been hypothesized to play a role in refractory/relapsing cancers, in the present work we investigated if autophagy could represent a constitutive cytoprotection mechanism for glioblastoma stem-like cells (GSCs) and if the modulation of autophagic process could affect GBM growth and survival. (nature.com)
  • Autophagy is a regulated mechanism of the cell that leads to the disassembly of unnecessary or dysfunctional components. (nature.com)
  • Hence, thanks to this complex mechanism, autophagy can provide energy supply to the cell and can represent a key cytoprotection mechanism allowing cell survival in unfavorable microenvironmental conditions such as those often found by cancer cells 7 . (nature.com)
  • Autophagy may represent a mechanism of resistance to oxidative stress induced by chemotherapeutic drugs and may potentiate cancer cell survival to hypoxia and nutrient starvation due to the frequently defective tumor vascularization. (nature.com)
  • Hence, we hypothesized that a combination of conjugated double bonds and an n-3 highly unsaturated fatty acid may produce a stronger antitumor effect, and we have previously shown that conjugated EPA (CEPA), prepared by alkaline treatment of EPA, induces strong and selective apoptosis in vitro and in vivo, with the mechanism proceeding via lipid peroxidation. (elsevierpure.com)
  • If lipid peroxidation contributes to the pathogenesis of retinopathy of prematurity and chronic lung disease, these results suggest an explanatory mechanism. (bmj.com)
  • Here, respiratory toxicity of BP-QDs has been validated in mice and human bronchial epithelial cells. (medworm.com)
  • Hydropic epithelial cell degenerations and moderate tubular dilatation were observed in some proximal and distal tubules. (fluoridealert.org)
  • Impact of long-term exposure to nanocellulose materials on the morphological transformation of human lung epithelial cells. (cdc.gov)
  • In this context, the aim of the present study was to evaluate the potential carcinogenic effect of cellulose nanocrystals (CNC) - one of the major forms of NC, using epithelial cells of pulmonary origin (BEAS-2B and A549). (cdc.gov)
  • together with protein concentration) and lens epithelial cells plus capsule (LECs/capsule) in patients with cataract (CAT), and also suffering from pseudoexfoliation syndrome (PEX), primary open-angle glaucoma (POAG) and pseudoexfoliation glaucoma (PXG). (molvis.org)
  • This necessitates the investigation of past experimental approaches in their attempt to correlate oxidative stress and ocular diseases in humans in both the epithelial cells plus the capsule (LECs/capsule) and in the aqueous humor (AH). (molvis.org)
  • In this study, we aimed to assess the neoplastic-like transformation potential of two forms of CNC derived from wood (powder and gel) in human pulmonary epithelial cells (BEAS-2B) in comparison to fibrous tremolite (TF), known to induce lung cancer. (cdc.gov)
  • The present study investigated the concentration of lipid peroxidation biomarkers in patients with OSA in comparison with nonapneic controls and the effects of results with nasal continuous positive airway pressure (nCPAP) on these biomarkers. (drcalapai.com)
  • 01). Stepwise regression analysis revealed that RDI was an independent significant predictor of all 3 lipid-peroxidation biomarkers. (drcalapai.com)
  • Moreover, we have found that TFs can increase the activity of superoxide dismutase (SOD) and reduce the level of malonaldehyde (MDA) together with lactate dehydrogenase (LDH) in H9c2 cells. (hindawi.com)
  • It is impossible to measure free radicals directly in vivo, and it is necessary to rely on the quantification of their reaction products such as protein carbonyls, modified DNA, and lipid peroxidation products. (bmj.com)
  • Although CNC exposures are associated with pulmonary inflammation and damage as well as oxidative stress responses and genotoxicity in vivo, studies evaluating cell transformation or tumorigenic potential of CNC's were not previously conducted. (cdc.gov)
  • In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
  • An alternative, pharmaceutical method employs the isotope effect on lipid peroxidation of deuterated polyunsaturated fatty acids (PUFAs) at the methylene bridges (bis-allylic sites) between double bonds, which leads to the inhibition of the chain reaction. (wikipedia.org)
  • This radical is also an unstable species that reacts with another free fatty acid, producing a different fatty acid radical and a lipid peroxide, or a cyclic peroxide if it had reacted with itself. (wikipedia.org)
  • This is the first such gene expression analysis of the effects of CEPA, and our results confirm that CEPA induces lipid peroxidation, activates mutant p53, and causes p53-dependent apoptosis in DLD-1 cells. (elsevierpure.com)
  • Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)
  • We demonstrated here that prolonged exposure (4 weeks) of the cells to occupationally relevant sub-toxic concentration (30 microg/cm2) of two forms of CNC (powder and gel) derived from wood, enhanced neoplastic-like transformation. (cdc.gov)
  • The hypothesis for this study was that lipid peroxidation, as evidenced by urinary MDA concentration, increases following blood transfusion. (bmj.com)
  • Zinc the investigation period were examined is a trace element required by the organism after delivery for NTD by a gynaecolo- for multiple biological functions, including gist and later the diagnosis was confirmed cell division, development and differentia- by a paediatrician. (who.int)
  • Lipid peroxidation and NOx levels of brain tissue increased significantly in group II and III compared to group I. On the contrary, GSH levels were significantly lower in group II and III than group I. However, no statistically significant alterations in any of the endpoints were noted between group II and Group III. (gazi.edu.tr)
  • Although pathology associated with lipid peroxidation is the dominant characteristic of the lesions examined, it is proposed that this process is secondary to necrosis of the adipose tissue. (int-res.com)
  • Taken together, these processes can activate signaling cascades leading to programmed cell death of nerve cells and surrounding tissue cells ( Oyinbo 2011 ). (scireproject.com)
  • Histologically the lungs of WPS-exposed mice had foci of mixed inflammatory cells infiltration in the interalveolar interstitium which consisted of neutrophils, lymphocytes and macrophages. (who.int)
  • In addition, end-products of lipid peroxidation may be mutagenic and carcinogenic. (wikipedia.org)
  • I am referring to taking fluoride internally, where it has been found to cause unscheduled DNA synthesis, sister chromatid exchanges and yes, mutagenic effects on the cells. (drkaslow.com)
  • destroys the surviving neurons' oligodendrocytes, which are critical to neuron protection because they are the glial cells that form the myelin sheath around axons in the central nervous system. (scireproject.com)
  • In the present study, for the first time, protective effects of three microalgal strains, including their extracts and active compounds, against both endogenous and exogenous AGEs in cell -based models were investigated. (rsc.org)
  • In addition, activation of the mutant p53 was also induced by CEPA, and these effects showed lipid-peroxidation dependency. (elsevierpure.com)
  • In the current work, the deleterious effects of Shiga toxin 2 and lipopolysaccharide are investigated in detail in hippocampal cells for the first time in a translational murine model, providing conclusive evidences on how these toxins may damage in the observed clinic cases. (biomedcentral.com)
  • The action of LPS has been described as an enhancer of the deleterious effects of Stx2 in different cells [ 4 ] and organs such as the brain [ 4 , 5 ]. (biomedcentral.com)
  • Effects of cell phone radiation on lipid. (gazi.edu.tr)
  • The protective effects of TFs resulted in higher expression of miR-21 in H/R-induced H9c2 cells than that of controls, which in turn upregulated Akt signaling activity via suppressing the expression of PTEN together with decreasing the ratio of Bax/Bcl-2, caspase3, and cleaved-caspase3 expression in H/R-induced H9c2 cells. (hindawi.com)
  • To test this hypothesis, we attempted to investigate whether TFs can exert its cardioprotective effect and the effects of TFs on miR-21 in H9c2 cells. (hindawi.com)
  • This experiment was designed to investigate the lipid peroxidation and histological effects of chronic fluorosis on first- and second-generation rat kidney tissues. (fluoridealert.org)
  • In this study, we examined the cytoprotective effects of cyanidin-3-glucoside (C3G) isolated from mulberry fruit against pancreatic β-cell apoptosis caused by hydrogen peroxide (H2O2)-induced oxidative stress. (spandidos-publications.com)
  • The MIN6 pancreatic β-cells were used to investigate the cytoprotective effects of C3G on the oxidative stress-induced apoptosis of cells. (spandidos-publications.com)
  • In the present study, we investigated the protective effects of C3G isolated from mulberry fruit against the H 2 O 2 -induced apoptosis of MIN6N pancreatic β-cells. (spandidos-publications.com)
  • establish mechanisms of action by studying the effects of the same chemicals in experimental animals and on human cells in vitro, allowing for a better prediction of human carcinogenicity and assessment of carcinogenic mechanisms. (who.int)
  • SOD, CAT and GPX activities were significantly increased in ellagic acid-treated V79-4 cells. (nutrimedical.com)
  • Lipid peroxidation was significantly higher in the chorion and amnion compared to the placenta. (karger.com)
  • In recent years, development of immunochemical detection of HNE-histidine adducts opened more advanced methodological possibilities for qualitative and quantitative detection of lipid peroxidation in various human and animal tissues[full citation needed] as well as in body fluids, including human serum and plasma samples. (wikipedia.org)
  • Once Stx2 reaches the circulation it may target endothelial, kidney and/or brain cells through the Stx2 globotriaosylceramide receptor (Gb3) causing cytotoxicity [ 1 ]. (biomedcentral.com)
  • The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cel embryos (p=0.04). (who.int)
  • As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)
  • Low-quality embryos, on the other cells, thereby maintaining a relatively low basal hand, frequently display morphological level [16-18]. (who.int)
  • Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
  • The toxicity of lipid hydroperoxides to animals is best illustrated by the lethal phenotype of glutathione peroxidase 4 (GPX4) knockout mice. (wikipedia.org)
  • We assessed the effect of Chop deletion in multiple mouse models of type 2 diabetes and found that Chop -/- mice had improved glycemic control and expanded β cell mass in all conditions analyzed. (jci.org)
  • Alternatively, indirect methods for assessing oxidative stress have been applied and focus mainly on the oxidative damage ROS cause in biomolecules, such are lipids, proteins, and DNA. (molvis.org)
  • These animals do not survive past embryonic day 8, indicating that the removal of lipid hydroperoxides is essential for mammalian life. (wikipedia.org)
  • Principle & Course of The ChromaFlash Chromatin Extraction Bundle accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. (accenx.com)
  • By comparing with previous studies, we speculate that primary cells generally are more sensitive to BP-QDs-induced damage than cancer cells. (medworm.com)
  • Anthracycline chemotherapy drugs damage the DNA in cancer cells, so they cannot reproduce. (medicalnewstoday.com)
  • Lycopene is a powerful antioxidant that might help protect cells from damage. (medlineplus.gov)
  • Taken together, our results suggest that C3G isolated from mulberry fruit has potential for use as a phytotherapeutic agent for the prevention of diabetes by preventing oxidative stress-induced β-cell apoptosis. (spandidos-publications.com)
  • High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)
  • Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
  • Cancer cells often maintain high basal level of ROS and are vulnerable to any further increase in ROS levels beyond a certain protective threshold. (degruyter.com)
  • Consequently, ROS-modulation has emerged as an anticancer strategy with synthesis of various ROS-inducing or responsive agents that target cancer cells. (degruyter.com)
  • This mini review highlights the development of compounds that target cancer cells by taking advantage of redox or metabolic alteration in cancer cells. (degruyter.com)
  • Cancer cells also enhance glucose-uptake to fuel these enhanced metabolic activities [ 2 ]. (degruyter.com)
  • This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)
  • This is because cancer cells maintain high basal level of ROS, thus they are vulnerable to any further increase in ROS (Fig. 1 ). (degruyter.com)
  • Anthracyclines work by damaging the DNA in cancer cells and interfering with their ability to reproduce. (medicalnewstoday.com)
  • The drugs bind with DNA to prevent cancer cells from reproducing. (medicalnewstoday.com)
  • Doctors select anthracycline drugs based on the type of cancer cells they want to target and how potent they need to be. (medicalnewstoday.com)
  • The critical challenge in cancer treatment is how to effectively kill cancer cells without affecting healthy cells. (hindawi.com)
  • The chemical products of this oxidation are known as lipid peroxides or lipid oxidation products (LOPs). (wikipedia.org)
  • On the other hand, it's unclear whether dietary lipid peroxides are bioavailable and play a role in disease, as a healthy human body has protective mechanisms in place against such hazards. (wikipedia.org)