Cells green fluorescent proteins. Medical search. Frequent questions

Plasmid transfection in bovine cells: Optimization using a realtime monitoring of green fluorescent protein and effect on gene reporter assay. (oregonstate.edu)
In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. (wikipedia.org)
It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest. (wikipedia.org)
Therefore, fusing the GFP gene to the gene of a protein of interest can significantly increase the protein's size and molecular mass, and can impair the protein's natural function or change its location or trajectory of transport within the cell. (wikipedia.org)
YUE Li-Li, JI Xi-Peng, DENG Yan-Hui, LV Li-Qun, DENG Xiao-Zhao.Fusion of HBV e Gene with Green Fluorescent Protein Gene and Its Expression in E. coli Cells .VIROLOGICA SINICA, 1997, 12(4): 336. (virosin.org)
HCT 116 colon cancer cells were exposed to purvalanol, which activated ER stress via upregulation of PERK, IRE1α gene expression, eIF-2α phosphorylation and ATF-6 cleavage at early time-points in the HCT 116 colon cancer cells. (spandidos-publications.com)
Activated and released IRE1α acts as an RNase to initiate transcription of XBP1 mRNA and it becomes a transcriptional activator for unfolded protein response (UPR) gene targets, such as BiP and calreticulin ( 10 ). (spandidos-publications.com)
A gene is a piece of DNA that has instructions for making a protein. (thetech.org)
During pilocarpine-induced SE, in vivo disruption of STEP activity allowed activation of the MAPK pathway, leading to immediate-early gene expression and significant rescue from cell death. (jneurosci.org)
Once the viruses and their gene cargoes infected liver cells, the infected cells expressed the gene and produced iRFP protein. (sciencedaily.com)
The team at the Central Institute for Experimental Animals in Kawasaki, Japan, added a fluorescent gene to the marmoset embryos, which were then transferred into surrogate females who produced five live births. (mentalfloss.com)
When they produced offspring, two passed the fluorescent gene onto their young. (mentalfloss.com)
Five beagles were created by cloning fibroblast cells that express a red florescent gene produced by sea anemones. (mentalfloss.com)
Two years later, a team at the same university bred a beagle name Tegon whose fluorescent gene could be controlled. (mentalfloss.com)
The gene Tri12 encodes a predicted Major Facilitator Superfamily protein suggested to play a role in export of trichothecene mycotoxins produced by Fusarium species. (usda.gov)
Then they characterized the three TPC proteins: MpTPC1 from the Type 1 TPC gene and MpTPC2 and MpTPC3 from the Type 2 TPC gene. (sciencedaily.com)
The modified virus contained an enhanced green fluorescent protein (EGFP) gene as a marker, which was named as NDV/Anh-EGFP. (usda.gov)
Common molecular biology applications include analysis of recombinantly expressed fluorescent proteins that have induced expression in various cell types to elucidate gene function or for in vivo cell tracking. (sigmaaldrich.com)
Profile and regulation of apolipoprotein E (ApoE) expression in the CNS in mice with targeting of green fluorescent protein gene to the ApoE locus. (lu.se)

A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)

The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP. (wikipedia.org)
In the 1960s and 1970s, GFP, along with the separate luminescent protein aequorin (an enzyme that catalyzes the breakdown of luciferin, releasing light), was first purified from the jellyfish Aequorea victoria and its properties studied by Osamu Shimomura. (wikipedia.org)
Remarkably, the GFP molecule folded and was fluorescent at room temperature, without the need for exogenous cofactors specific to the jellyfish. (wikipedia.org)
GFP, which scientists first isolated in jellyfish, is a protein that changes light from one color into another. (eurekalert.org)
For the past 20 years, scientists have used a variety of colored fluorescent proteins, derived from jellyfish and corals, to visualize cells and their organelles and molecules. (sciencedaily.com)
When these sheep were born in October 2012, scientists at the Animal Reproductive Institute of Uruguay immediately injected them with a green protein found in the Aequorea Victoria jellyfish. (mentalfloss.com)
A research institute in France actually created the rabbit for Kac - the florescent jellyfish protein was injected into a fertilized rabbit egg - and later hesitated over releasing the animal due to protests from animal rights groups over Alba's very creation. (mentalfloss.com)
Scientists added DNA from fluorescent jellyfish to more than 260 pig embryos, which were then implanted into eight different sows, four of which became pregnant. (mentalfloss.com)
Green fluorescent protein (GFP) has been busy in the 1990s, having been removed from the jellyfish Aequora victoria and transfected into just about every type of cell possible. (the-scientist.com)

The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. (wikipedia.org)
Although this near-wtGFP was fluorescent, it had several drawbacks, including dual peaked excitation spectra, pH sensitivity, chloride sensitivity, poor fluorescence quantum yield, poor photostability and poor folding at 37 °C (99 °F). The first reported crystal structure of a GFP was that of the S65T mutant by the Remington group in Science in 1996. (wikipedia.org)
mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins. (nature.com)
Intensiometric genetically encoded biosensors, based on allosteric modulation of the fluorescence of a single fluorescent protein, are powerful tools for enabling imaging of neural activities and other cellular biochemical events. (nature.com)
After designing and building a GFP UV reporter protein (eYGFPuv) that provides enhanced signals for all tested plant species, the researchers demonstrated that strong fluorescence could be captured using either a fluorescence microscope or UV light. (eurekalert.org)
After 72 h, green fluorescent protein (GFP)-positive cells were counted by fluorescence-activated cell sorter analysis. (cdc.gov)
HSV-1g fluorescence was reduced by 66.3% and 65.4% in HCE-T and Vero cells, respectively, after treatment with 0.4 µg/ml SPS. (molvis.org)
Furthermore, the viral fluorescence intensities were inhibited by SPS in a dose-dependent manner when the viruses or cells were preincubated with SPS. (molvis.org)
Each cell or particle is analyzed by visible light scatter or by fluorescence as cells rapidly flow past each laser. (sigmaaldrich.com)
Independent of light scatter analysis, fluorescence measurements are achieved by transfection and expression of common fluorescent proteins (e.g. (sigmaaldrich.com)
FA vapor generated from a saturated FA solution induced a norepinephrine fluorescence in adrenal medullary cells whose intensity was almost beyond belief compared to what was known then. (lu.se)
The original Falck-Hillarp method was based on the exposure of freeze-dried tissue to formaldehyde vapor, allowing dopamine, noradrenaline and serotonin to be converted to molecules that emit a yellow-green fluorescence in the microscope. (lu.se)

Understanding how embryonic cells biomolecularly restrict their early developmental potential and promote specific cell commitment helps to provide key insights on cancer plasticity and could improve fertilization protocols. (zeiss.com)

The objective of this study was to investigate the optimal conditions to generate reliable GRT assay data on bovine immortalized cell lines, Madin Darby Bovine Kidney (MDBK) and bovine mammary epithelial alveolar (MACT) cells. (oregonstate.edu)
N/N1003A lens epithelial cells, bovine primary lens epithelial cells, and stably transfected N/N1003A lens epithelial cells were used. (nih.gov)
Cav-1 and -2 were found in N/N1003A and bovine primary lens epithelial cells. (nih.gov)
SCARA5 expression is significantly downregulated in various tumor tissues including liver cancer (LC), rectal cancer, and glioblastoma [ 15 , 16 ] and might affect cell proliferation and invasion abilities by the focal adhesion kinase (FAK) signaling pathway and epithelial-mesenchymal transition (EMT) [ 5 , 17 - 20 ]. (hindawi.com)
The expression of the epithelial cell adhesion molecule (EpCAM) is correlated with a poor prognosis, and treatment failure early tumor recurrence of hepatocellular carcinoma (HCC) patients. (copgfp.com)
TAZ is responsive to TGFβ signal in conjunctiva epithelial cells. (molvis.org)
Cell lysate of the epithelial cells with or without TGFβ treatment were blotted by anti-TAZ. (molvis.org)
8 Astrocytes interact with blood vessels, pericytes, and epithelial cells to organize CNS flow and modulate the blood-brain barrier (BBB). (news-medical.net)
ABOVE: These cultured human lung epithelial cells have taken up particles (yellow) that carry mRNA encoding green fluorescent protein. (the-scientist.com)
Synovial sarcomas encompass both biphasic (epithelial and spindle cell components) and monophasic (purely spindle cell) variants. (medscape.com)

Overexpression of PKCgamma-enhanced green fluorescent protein (EGFP) increased the interaction of PKCgamma-EGFP with Cav-1 and Cx43 and decreased gap junction Cx43 plaques without exogenous growth factors. (nih.gov)
The FluorImager also works fine to detect EGFP expression in animal cells. (bio.net)
NDV/Anh-EGFP significantly inhibited liver cancer development both in cancer cell lines and in mice. (usda.gov)
The modified virus contained an insertion of enhanced green fluorescent protein (EGFP), named NDV/Anh-EGFP. (usda.gov)

Scientists Roger Y. Tsien, Osamu Shimomura, and Martin Chalfie were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein. (wikipedia.org)
A decade ago, scientists successfully developed sperm in one animal that had come from cells in another. (livescience.com)
The scientists collected fluorescent green rat sperm from the mice and injected them into rat eggs. (livescience.com)
Scientists can take advantage of this by using proteins found in bacteria called "restriction enzymes. (thetech.org)
At the Society for Neuroscience (SfN) annual meeting, which drew nearly 25,000 scientists to San Diego November 9-14, researchers reported new data on the fundamental roles played by glial cells in forming and refining neural circuits. (alzforum.org)
Other scientists outlined new approaches to promote a kind of glial cell activation that might help mice tackle Alzheimer's-like pathology in the brain (see part two of this series). (alzforum.org)
Researchers have developed the first fluorescent protein that enables scientists to clearly "see" the internal organs of living animals without the need for a scalpel or imaging techniques that can have side effects or increase radiation exposure. (sciencedaily.com)
Scientists from the Institut Pasteur and the CNRS set out to investigate the consequences of SARS-CoV-2 infection for cell function and the antiviral role of innate immunity. (pasteur.fr)
Scientists from the Virus and Immunity Unit (Institut Pasteur/CNRS) investigated viral fusion, the mechanism by which viral membranes and cellular membranes fuse, allowing viral genetic material to enter the target cell. (pasteur.fr)
Using real-time video microscopy, the scientists demonstrated that infected cells fuse with neighboring cells to form syncytia, or giant cells containing dozens of virus-producing cells, which eventually die. (pasteur.fr)
The scientists demonstrated that in cells infected with SARS-CoV-2, IFITM proteins inhibit syncytium formation, thereby giving us an insight into the way in which interferon might control the evolution of COVID-19. (pasteur.fr)

Frederick Tsuji's lab independently reported the expression of the recombinant protein one month later. (wikipedia.org)
Coimmunoprecipitation and Western blot analysis were used to detect protein expression and their interactions. (nih.gov)
Lamin A/C cumulative protein expression of all variants is reported by semi-quantitative Western blotting. (mdpi.com)
Green fluorescent protein expression is seen in the offspring. (livescience.com)
Thus, we conclude that As2O3 can reduce metastasis potential of NPC cells, involving inhibition of MMP-9 expression. (scielo.br)
Expression of TAZ protein was upregulated in TGFβ treated cells. (molvis.org)
It has recently been shown that murine intestinal macrophages are continually repopulated from circulating blood monocytes, identified by expression of the cell surface markers Ly6C, CCR2, and CD62L ( 6 ). (frontiersin.org)
Flow cytometry antibodies are used to specifically bind to different cell markers, helping to characterize the cell type and assess any changes in protein expression as part of the experiment. (sigmaaldrich.com)
By utilising the expression of these two reporter proteins, P. falciparum can be successfully visualised and quantified with high sensitivity. (nagasaki-u.ac.jp)
Then, ALDH2 expression was promoted and suppressed to explore the role of ALDH2 in OGD/R-treated cells. (bvsalud.org)
Results In OGD/R-treated cells, ALDH2 expression was decreased, which was due to the hypermethylation of ALDH2 in the promoter region. (bvsalud.org)
Augmented miR-30a-5p expression was induced by hypoxia induced factor 1α (HIF-1α) in ICCA cell. (bvsalud.org)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)

Endoplasmic reticulum (ER) is an essential organelle responsible for protein synthesis, folding, post-translational modification of proteins and protein trafficking in eukaryotes ( 8 ). (spandidos-publications.com)
Interferon is a substance with antiviral activity that stimulates cells' natural defenses by inducing the synthesis of proteins that protect them from infection. (pasteur.fr)

The 'GFP sandwich' works as a solar panel because the strands of the protein works itself between the two electrodes and when UV light is applied, the fluorescent protein absorbs photons and emits electrons. (reefbuilders.com)
This new phytochrome-based fluorescent protein, dubbed iRFP, both absorbs and emits light in the near-infrared portion of the electromagnetic spectrum- the spectral region in which mammalian tissues are nearly transparent. (sciencedaily.com)
A few hours after an infection, the body emits an alarm signal, interferon, enabling cells that have not yet been infected to produce antiviral proteins. (pasteur.fr)
These living lasers are fundamentally different from cells that simply make fluorescent proteins: by definition, a laser emits a strong, coherent beam of light. (technologyreview.com)

The Hep3B and HepG2 cell subpopulations in terms of EpCAM-low and high EpCAM was evaluated in an orthotopic model of athymic mice. (copgfp.com)
ASCs were harvested from the fat tissues of transgenic mice that express a green fluorescent protein (GFP-ASCs) and then injected into the peri-implant space of recipient mice. (biomedcentral.com)
In particular, we used ASCs harvested from transgenic mice that produce a green fluorescent protein (GFP) in all of their cells to track the implantation and differentiation of ASCs around the implant [ 19 ]. (biomedcentral.com)
Shinohara and his colleagues then removed the stem cells that sperm arise from in the rats and implanted them into testicles of mice. (livescience.com)
Unlike wild-type cells, astrocytes in the MEGF10- and MERTK-deficient mice ingested no synaptic components and the visual system of these animals failed to mature, suggesting that phagocytosis of synapses contributes to development of the retina. (alzforum.org)
The mice were then exposed to near-infrared light and it was possible to visualize the resulting emitted fluorescent light using a whole-body imaging device. (sciencedaily.com)
We show that the monocyte:macrophage balance is disrupted in colon inflammation to favour recruitment of CD14 + HLA-DR Int cells in humans, and Ly6C Hi monocytes in mice. (frontiersin.org)
The antitumor efficacy of the recombinant virus on hepatoma was examined both in cell cultures and in mice. (usda.gov)
Moreover, our group has recently evidenced that early-life infection in 5xFAD mice induces a subpopulation of microglial cells with a monocyte-like profile presenting as potential markers genes corresponding to Gal3, and APOE. (lu.se)
I also describe the development of a novel behavioural task that is predictive of mesDA neuron cell loss in mice. (lu.se)

However, this technology relies heavily on the insertion of foreign plasmid DNA into the nuclei of cells (i.e., transfection), which can be very challenging and highly variable among cell types. (oregonstate.edu)
At each original cell body of the seam syncytium, where the nuclei are positioned, the seam completely interrupts hyp 7 (section through B in inset ), whereas at the ends of the cells, the seam is so thin that hyp 7 runs behind it, enfolding it completely (section through C in inset ). (wormatlas.org)
DSRCT is composed of small cells with round hyperchromatic nuclei and a dense fibrous or spindle cell stroma. (medscape.com)

This book chapter will comment on fluorescent reporter proteins and nanocrystals' applicability as fluorescent markers. (intechopen.com)
Fluorescent reporter proteins in the Drosophila model system offer a degree of specificity that allows monitoring cellular and biochemical phenomena in vivo, such as autophagy, mitophagy, and changes in the redox state of cells. (intechopen.com)
The plant on the right contains the eYGFPuv reporter and glows green under UV illumination. (eurekalert.org)
This transgenic P. falciparum reporter line constantly expresses two reporter proteins, GFP and NanoLuc, at high levels in two different hosts, human and mosquito. (nagasaki-u.ac.jp)
We have created a transgenic P. falciparum that expresses two reporter proteins, GFP and NanoLuc, at high levels in both the human- and the vector mosquito stage. (nagasaki-u.ac.jp)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)

GFP is the most common fluorescent pigment found in soft and stony corals, GFP is an antioxidant when corals are exposed to light stress and some corals are literally being cross infected by GFP from other corals. (reefbuilders.com)

Proteins are molecules that are involved in almost everything a cell does. (thetech.org)
Molecules such as phosphatidylinositol-3,5-bisphosphate and nicotinic acid adenine dinucleotide phosphate, that activate the TPCs of mammalian cells, failed to affect the ion channel activity in isolated vacuoles of the mutant plants. (sciencedaily.com)
Numerous types of labels can be used to visualize the antibodies for detection in the flow cytometer depending on the experimental design including nucleic acid dyes, cell viability dyes, polymer dyes, quantum dots, small organic molecules, and fluorescent proteins. (sigmaaldrich.com)
The inner fluid can contain cargo molecules such as nucleic acids or soluble proteins. (biomedcentral.com)
Individual bacterial and yeast cells often produce molecules that are used by others. (cdc.gov)
More recently, the realization that macro- principal experimental challenge is to resolve and quantify molecules occupy 20-30% of the intracellular volume has populations of protein oligomers in dynamic equilibrium. (lu.se)

What happens if we inhibit protein degradation during the Maternal to Zygotic Transition? (stowers.org)

Adult hair follicle bulge-derived stem cells (HFBSCs) has a capacity of neuronal differentiation, it is easy to harvest and relatively immune-privileged, which makes them potential candidates for autologous stem cell-based therapies. (copgfp.com)
Adipose-derived stem cells (ASCs) have been reported to reduce fibrosis in various tissues. (biomedcentral.com)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)
Here we describe how this system can be used for human embry- onic stem cells. (lu.se)

Innate immunity occurs rapidly and can act in just a few hours, well before the other two forms of immunity - humoral immunity (the production of neutralizing antibodies that block virus entry into cells) and cell-mediated immunity (involving white blood cells such as cytotoxic lymphocytes that are capable of destroying infected cells). (pasteur.fr)
Green Fluorescent Protein, GFP) and staining with fluorescently conjugated antibodies or fluorescent dyes. (sigmaaldrich.com)
View ColorWheel® protocol steps for flow cytometry analysis when using ColorWheel® antibodies with ColorWheel® dyes including antibody preparation, PBMC sample preparation, cell surface staining, and intracellular (cytoplasmic) staining. (sigmaaldrich.com)
A large number of the Prestige Antibodies have been used in subcellular localization studies by immunofluorescence (IF) staining of three cell lines: A-431, U-2 OS, and U-251MG. (sigmaaldrich.com)

Reproductive biologist Takashi Shinohara at Kyoto University in Japan and his colleagues first began with rats genetically engineered to produce a green fluorescent protein. (livescience.com)

While other researchers had bred partially fluorescent pigs, these genetically modified pigs had not only glowing skin and eyes, but also organs, including the heart. (mentalfloss.com)
First, the researchers genetically modified human liver cells so that they produce large amounts of green fluorescent proteins that are scattered throughout the cell. (technologyreview.com)
In this study, we genetically modified P. falciparum to simultaneously express GFP, a fluorescent protein, and NanoLuc, a luciferase. (nagasaki-u.ac.jp)

Most commercially available genes for GFP and similar fluorescent proteins are around 730 base-pairs long. (wikipedia.org)
Therefore, it is important to explore candidate genes that could have significance to the clinical diagnosis, as well as the treatment of esophageal squamous cell carcinoma (ESCC) at the molecular level [ 4 ]. (hindawi.com)
This in turn shows how cells deliver and use genes. (eurekalert.org)
Typically, these green fluorescent proteins are used to monitor the activity of altered genes. (mentalfloss.com)
To do so, the researchers first performed a phylogenetic analysis of the TPC genes in the green plant lineage. (sciencedaily.com)

A strain having Tri12p linked to green fluorescent protein shows localization of the protein consistent with the plasma membrane. (usda.gov)
Tagging these proteins with a fluorescent marker, they studied their localization in M. polymorpha cells. (sciencedaily.com)

Stem cell transplantation may lead to recovery of function through different mechanisms, such as the replacement of cells through differentiation, angiogenesis stimulation and support for micro environment. (copgfp.com)
Rat offspring from a mouse recipient that received cryopreserved rat testis cell transplantation. (livescience.com)
This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)
We still do not know what specific factors contribute to the success in transplantation i.e. what cells are responsible for motor recovery? (lu.se)
The aim of this thesis was to understand how particular factors such as neuronal content, placement and cell source, affect functional outcome after transplantation into the rodent brain. (lu.se)

Cyclin-dependent kinases (CDKs), are members of the serine-threonine protein kinase family and are responsible for taking control of cell cycle regulation in eukaryotic cells. (spandidos-publications.com)
Eukaryotic cells have elaborate cell signaling and trafficking systems based on different kinds of vesicle structures and membrane organelles. (biomedcentral.com)
Moreover, we revealed that the packing of miR-30a-5p into ICCA cells-derived exosomes was mediated by eukaryotic translation initiation factor 4B (EIF4B). (bvsalud.org)

Although the molecular mechanisms responsible for the selective vulnerability of these cells are not well understood, activation of the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway has been implicated in neuroprotective responses to excitotoxicity in other neuronal populations. (jneurosci.org)
A key finding was that SE stimulated acute and robust MAPK pathway activation in SE-resistant granule cells but not in vulnerable populations of hilar interneurons. (jneurosci.org)
Myeloid cell populations isolated from colonic inflammatory bowel disease (IBD) or murine dextran sulphate sodium (DSS) induced colitis were assessed using flow cytometry and compared to healthy controls. (frontiersin.org)
Numerous fields of study use flow cytometry and often analyze multiple parameters within diverse populations of cells. (sigmaaldrich.com)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)

These capsules typically represent dense fibrous connective tissue containing foreign giant cells or granulomas, suggesting that they are caused by inflammatory responses [ 6 ]. (biomedcentral.com)
The bilayer is composed of phospholipids and is typically embedded with membrane proteins. (biomedcentral.com)

The breakthrough marks the first time researchers produced healthy offspring [Photo] from sperm cells fostered in a different species. (livescience.com)
Attaching it to other proteins allows researchers to find out if cells produce those proteins and where within cells to find them. (eurekalert.org)
In this study, researchers describe how a special type of GFP can be used to 'see' protein production with the unaided eye. (eurekalert.org)
Using confocal microscopy, the researchers saw astrocytes engulfing axon terminals and synapses on these retinal cells. (alzforum.org)
The researchers targeted their fluorescent protein to the liver -- an organ particularly difficult to visualize because of its high blood content. (sciencedaily.com)
The researchers also found that the three TPC proteins were primarily localized at the vacuolar membrane of M. polymorpha. (sciencedaily.com)
A laser based on living cells has been created by researchers at Harvard Medical School and the Massachusetts General Hospital in Boston. (technologyreview.com)
The researchers pumped the living lasers by pulsing the cells with light through a microscope. (technologyreview.com)

To overcome that roadblock, the laboratory of Vladislav Verkhusha, Ph.D., associate professor of anatomy and structural biology at Einstein and the study's senior author, engineered a fluorescent protein from a bacterial phytochrome (the pigment that a species of bacteria uses to detect light). (sciencedaily.com)
Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines. (sigmaaldrich.com)
Intracellular membrane structures are rare in bacteria, thus making them ideal chasses for cell-based vesicle production. (biomedcentral.com)

These original observations, obtained in in vitro models with IFITM overexpression, now need to be reproduced in physiological models of human bronchial cells. (pasteur.fr)
ALDH2 overexpression improved cell viability and ALDH2 knockdown suppressed cell viability in OGD/R-treated cells. (bvsalud.org)
We also found that ALDH2 overexpression attenuated OGD/R-induced cell apoptosis, pyroptosis, ferroptosis and autophagy, while ALDH2 knockdown facilitated the OGD/R-induced cell apoptosis, pyroptosis, ferroptosis and autophagy. (bvsalud.org)

Using high, medium, and low transfection efficiency conditions determined in Experiment 1, we performed a GRT assay for peroxisome proliferator-activated response element (PPRE) luciferase in MACT and MDBK cells treated with 10nM or 100nM of synthetic Peroxisome Proliferator-activated Receptor β/σ (PPARβ/σ) agonist. (oregonstate.edu)
Baird, G. S., Zacharias, D. A. & Tsien, R. Y. Circular permutation and receptor insertion within green fluorescent proteins. (nature.com)
ApoE interacts with multiple ligands, among them triggering-receptor expressed on myeloid cells 2 (TREM2), which is greatly implicated in neuroinflammation [5]. (lu.se)
[ 6 ] A majority of these tumors will exhibit polysomy of the epidermal growth factor receptor ( EGFR ) genetic locus on chromosome 7 in subsets of tumor cells. (medscape.com)

Here, we used recombinant VHH directed against human glial fibrillary acidic protein (GFAP), a specific marker of astrocytes. (open.ac.uk)
To analyze their ability to be used as a specific transporter, we then expressed a recombinant fusion protein VHH-green fluorescent protein (GFP). (open.ac.uk)
There was no obvious difference of the growth capability between the recombinant NDV and the parental virus in the cancer cells. (usda.gov)

It is unclear the reason for the low transfection in MDBK cells. (oregonstate.edu)
Maximal transfection efficiency was obtained with 0.3μL/well of LipoLTX plus 25ng/well of plasmid DNA (ca. 29.5±1.9%) and 0.15μL/well of LipoLTX plus 25ng/well of plasmid DNA (ca. 4.0±0.4%) for MACT and MDBK cells, respectively. (oregonstate.edu)
The GRT assay was unaffected by poor transfection in MACT cells although the high transfection hampered the possibility of detecting differences between 10 and 100nM of the PPARβ/δ agonist. (oregonstate.edu)

Here, we have systematically investigated the role of EpCAM + cancer cells in tumor initiation in an orthotopic model of HCC. (copgfp.com)
FACS-sorted cells were inoculated into the left lobe of the liver, and the tumor growth was monitored by high frequency ultrasound. (copgfp.com)
Cancer cell-derived exosomes carrying microRNAs involves in tumor microenvironment (TME) regulation. (bvsalud.org)

The bifunction fusion protein of hepatitis B virus e antigen and mutant green fluorescent protein was expressed in E. coli BL21 strain. (virosin.org)
The fusion protein was separated and its anti-genecity was determined by ELISA kit for HBe, meanwhile the intensity of its luminescence could be observed. (virosin.org)
The approach in which the antigen was tagged by equal molecular GFP allows using the bi-function fusion protein to establish a new method for immunogical diagnosis. (virosin.org)
These "fluobodies" specifically labeled GFAP on murine brain sections, and a basic variant (pI=9.3) of the fusion protein VHH-GFP was able to cross the BBB and to label astrocytes in vivo. (open.ac.uk)
A P9-GFP fusion protein was used to study the targeting of heterologous proteins into P9 vesicles. (biomedcentral.com)
Production of the GFP-tagged P9 vesicles required P12, which protected the fusion protein against proteolytic cleavage. (biomedcentral.com)

Green: fluorescent marker of cell fusion. (pasteur.fr)
Red: fluorescent marker of cell death. (pasteur.fr)

Rat primary astrocytes were isolated from different brain regions (cortex, cerebellum, and hippocampus) and grown in vitro to demonstrate the utilization of live-cell analysis for monitoring and comparing the diversity of cultured astroglia phenotypes. (news-medical.net)
Cortex, Hippocampus, and Cerebellum astroglia were seeded in 96-well plates at 2,000 cells/well. (news-medical.net)

80% of the GABAergic cells that were lost ( Buckmaster and Jongen-Rélo, 1999 ). (jneurosci.org)

We have developed pH-sensitive mutants of green fluorescent protein ('pHluorins') by structure-directed combinatorial mutagenesis, with the aim of exploiting the acidic pH inside secretory vesicles to monitor vesicle exocytosis and recycling. (nih.gov)

Although purvalanol-induced cell cycle arrest and apoptotic cell death were demonstrated in prostate ( 5 ), breast ( 6 ) and colon cancer cells ( 7 ), the exact molecular mechanism of purvanol-induced apoptosis has not been elucidated yet. (spandidos-publications.com)
Mechanism of Notch Signaling: The image depicts a type of cell signaling, in which two animal cells interact and transmit a molecular signal from one to the other. (asu.edu)
Given these observations, insight into the molecular mechanism responsible for the subclass-specific vulnerability of interneurons to SE-induced cell death may provide a deeper understanding of temporal lobe epileptogenesis. (jneurosci.org)
Information regarding the molecular events underlying cell type-specific vulnerability may be gained by the analysis of inherent neuroprotective signaling responses actuated by excitotoxic stimuli. (jneurosci.org)
Optical imaging labels can report on the molecular workings of tissues and cells in the body. (technologyreview.com)

Along these lines, accumulating evidence shows that the p42/44 mitogen-activated protein kinase (MAPK) pathway plays an important role in neuronal cell survival. (jneurosci.org)
Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)
Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)

When linked to a vesicle membrane protein, pHluorins were sorted to secretory and synaptic vesicles and reported transmission at individual synaptic boutons, as well as secretion and fusion pore 'flicker' of single secretory granules. (nih.gov)
Nasopharyngeal carcinoma (NPC) is notorious for the metastases, which are in close association with Epstein-Barr virus-encoded latent membrane protein 1 (LMP1). (scielo.br)

Optical indicators provide a powerful means to reveal such distributed activity, particularly when protein-based and encodable in DNA: encodable probes can be introduced into cells, tissues, or transgenic organisms by genetic manipulation, selectively expressed in anatomically or functionally defined groups of cells, and, ideally, recorded in situ, without a requirement for exogenous cofactors. (nih.gov)

Together, our work demonstrates ILK as necessary for normal oligodendrocyte development, reinforces its role as a bridge between the actin cytoskeleton and cell membrane, and highlights the overarching compensatory capacity of oligodendrocytes in response to cellular milieu. (jneurosci.org)
But interferon counters this phenomenon by inducing cellular proteins that prevent the fusion of infected cells. (pasteur.fr)
The Incucyte ® Live-Cell Analysis System delivers flexible kinetic quantification of biological phenotypes to monitor growth, motility, morphology, and cellular function. (news-medical.net)
In quick succession, the preimplantational mouse embryo undergoes a series of cellular divisions involving two critical cell fate decisions. (zeiss.com)
To determine which cells are expressing specific cellular markers and how these cells are organized relative to each other requires volumetric imaging of the pre-implantation embryos by confocal microscopy. (zeiss.com)

By uncovering the role of a previously unknown protein-protein interaction in glioblastoma, we were able to develop a designer peptide which possesses robust therapeutic efficacy in treating all major types of glioblastoma in preclinical models," says Dr. Xi Huang , a Senior Scientist in the Developmental & Stem Cell Biology program . (innovations-report.com)
The use of real-time, live-cell analysis provides new possibilities and cell biology workflows. (news-medical.net)
Fluorescent protein tags that emit visible or infrared light are now common tools for studying cell biology in test tubes. (technologyreview.com)

To do this, we need to combine the human instructions for how to make the protein with bacterial instructions for where and how much to make. (thetech.org)
Outside of these intriguing examples, intracellular membranes are rare in the majority of bacterial cells, making them attractive systems for cell-based vesicle production. (biomedcentral.com)

The reason: hemoglobin in an animal's blood effectively absorbs the blue, green, red and other wavelengths used to stimulate standard fluorescent proteins along with any wavelengths emitted by the proteins when they do light up. (sciencedaily.com)

Flow cytometry is a technology that uses single or multiple lasers to provide a multi-parametric analysis of single cells. (sigmaaldrich.com)
CCK-8 assay was applied to detect cell viability, and flow cytometry was applied to evaluate cell apoptosis. (bvsalud.org)
Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)

The messenger RNA (mRNA) carried instructions for making a bioluminescent protein. (the-scientist.com)

The lab of Martin Chalfie expressed the coding sequence of wtGFP, with the first few amino acids deleted, in heterologous cells of E. coli and C. elegans, publishing the results in Science in 1994. (wikipedia.org)
Here we provide an overview of a cell-free TX-TL system that utilizes the native Escherichia coli TX-TL machinery, thereby allowing a large repertoire of parts and networks to be characterized. (biorxiv.org)
This study evaluated the minimum requirements for the formation of phi6-specific vesicles and the possibility to localize P9-tagged heterologous proteins into such structures in Escherichia coli . (biomedcentral.com)
Using transmission electron microscopy, we detected membranous structures in the cytoplasm of E. coli cells expressing P9. (biomedcentral.com)
Our results demonstrate that the phi6 major envelope protein P9 can trigger formation of cytoplasmic membrane structures in E. coli in the absence of any other viral protein. (biomedcentral.com)

The possibility to locate heterologous proteins into the P9-lipid vesicles facilitates the production of vesicular structures with novel properties. (biomedcentral.com)

Cell surface gap junction Cx43 plaques were detected by confocal microscopy. (nih.gov)
Using real-time video microscopy, they showed that infected cells in culture can fuse with neighboring cells and die after forming giant cells known as "syncytia," composed of dozens of other cells. (pasteur.fr)

In May 2000, Kac introduced the world to his ' GFP bunny ,' an albino rabbit named Alba that glowed fluorescent when exposed to blue light. (mentalfloss.com)

Concurrently, cell viability was assayed by using Cell virusreplicationinvitro,butonlyatcytotoxicconcentrations. (cdc.gov)
Fifteen micromoles of purvalanol induced a reduction in cell viability by 20 and 35% within 24 and 48 h, respectively. (spandidos-publications.com)

Purvalanol is a purine-derived CDKI that binds with a high selectivity and competitively to the ATP binding side of CDK1/2 and leads to G2/M cell cycle arrest ( 4 ). (spandidos-publications.com)
C1q is a lectin-like protein that binds apoptotic cells. (alzforum.org)

Earlier work in the lab had revealed that the star-shaped cells express high levels of phagocytic receptors. (alzforum.org)

Fluorescent indicators for Ca 2+ based on green fluorescent proteins and calmodulin. (nature.com)

SCARA5 promoted cell proliferation and migration but inhibited apoptosis in ESCC. (hindawi.com)
New anticancer therapy strategies refer to the inhibition of CDK-cyclin complexes as an important target to prevent uncontrolled proliferation and induce apoptosis in cancer cells ( 2 ). (spandidos-publications.com)
Natural polyamines, putrescine, spermidine and spermine, play essential roles in the regulation of cell growth and proliferation. (spandidos-publications.com)
Further exploration revealed that ICCA-derived miR-30a-5p could be transferred to endothelial and increased endothelial cells recruitment and proliferation, induced angiogenesis and vascular permeability in exosome dependent manner. (bvsalud.org)
Acute myeloid leukemia (AML) is a deadly hematological malignancy characterized by oncogenic translational addiction that results in over-proliferation and apoptosis evasion of leukemia cells. (bvsalud.org)

As the brain ages, those functions seem to get creaky, raising the possibility that keeping glial cells young and fit could protect against neurodegeneration. (alzforum.org)
Won-suk Chung , a postdoc in in Ben Barres' lab at Stanford School of Medicine, Palo Alto, California, studies astrocytes-the most common glial cell in the brain. (alzforum.org)
Astrocytes are specialized glial cells ubiquitously distributed throughout the spinal cord and brain. (news-medical.net)

Tissues and Cells. (lu.se)

cOmplete ULTRA Tablets, EDTA-free contain the most powerful protease inhibitor cocktail for the optimal protection of your proteins against protease degradation. (sigmaaldrich.com)

Lamin A/C proteins have key roles in nuclear structural integrity and chromosomal stability. (mdpi.com)
Interferon-induced transmembrane proteins (IFITMs) can play contradictory roles in different coronaviruses. (pasteur.fr)

Viral fusion can also occur when the cell is infected and produces new viruses. (pasteur.fr)
The cystovirus Pseudomonas phage phi6 has an envelope that harbors five viral membrane proteins and phospholipids derived from the cytoplasmic membrane of its Gram-negative host. (biomedcentral.com)

Here, we report that high levels of the striatal-enriched protein tyrosine phosphatase (STEP), a key regulator of ERK/MAPK signaling, are found in vulnerable somatostatin-immunoreactive hilar interneurons. (jneurosci.org)
These findings identify a key set of signaling events that render somatostatinergic hilar interneurons vulnerable to SE-induced cell death. (jneurosci.org)
Susceptibility to SE-induced cell death has been shown to vary among phenotypically distinct subgroups of hilar interneurons. (jneurosci.org)

Greenwald, E. C., Mehta, S. & Zhang, J. Genetically encoded fluorescent biosensors illuminate the spatiotemporal regulation of signaling networks. (nature.com)
Given the stringent, cell type-specific regulation of this pathway, these data raise the possibility that vulnerability to SE-induced cell death may, in part, depend on the capacity of a cell to elicit a MAPK pathway-dependent neuroprotective response. (jneurosci.org)
Hancock, 2004), and cell volume regulation (Garner and technique known as magnetic relaxation dispersion (MRD). (lu.se)

During development, oligodendrocyte precursor cells (OPCs) differentiate into oligodendrocytes (OLs) that extend processes to contact multiple axons of CNS neurons. (jneurosci.org)
They determined that EAG2-Kvβ2 interaction is required for neurons to communicate with glioblastoma cells, facilitating tumour growth, invasion and chemoresistance. (innovations-report.com)
SE-induced MAPK activation within these neurons was repressed by STEP, leading to cell death. (jneurosci.org)
Methods In vitro oxygen glucose deprivation/reoxygenation (OGD/R) model was established in HT22 cells and mouse cortical neurons. (bvsalud.org)
CRT aims to replace neurons that have degenerated in PD, with donor cells that have the potential to functionally re-integrate into the host circuitry. (lu.se)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)

Western blot was applied to detect the apoptosis-related proteins (Caspase 3, Bcl-2 and Bax), necroptosis-related proteins (RIP3 and MLKL), pyroptosis-related proteins (NLRP3 and GSDMD), ferroptosis-related protein (ACSL4 and GPX4), and autophagy-related proteins (LC3B, and p62). (bvsalud.org)

Their widely recognized and appreciated article, which discusses this study in detail, was first published online in December 2021 and subsequently in print in the February issue in Plant and Cell Physiology. (sciencedaily.com)

In neural systems, information is often carried by ensembles of cells rather than by individual units. (nih.gov)

The cocktail comprises oncogenic translation inhibitor HHT, drug efflux pump P-gpi ENC and anti-apoptotic protein Bcl-2i VEN. (bvsalud.org)

Murine leukemia virus (MLV) or HIV pseudotypes bearing either the pantropic vesicular stomatitis virus envelope protein (VSV-G) as a positive control, or the SARS-CoV S, were added to target cells. (cdc.gov)
The phi6 major envelope protein P9 and the non-structural protein P12 are essential for the envelopment of its virions. (biomedcentral.com)
Early studies on nonsense mutants of phage phi6 suggested that the major envelope protein P9 and the non-structural protein P12 are the only proteins needed for phi6 virion envelopment [ 23 ]. (biomedcentral.com)

They want to change what a protein does, where it is found or how much of it gets made. (thetech.org)
We examined these two proteins closely and found that when they interacted they created a potassium channel complex that is fundamental to the aggressive nature of the cancer," says Dong, a former PhD student and current post-doctoral fellow in the Huang Lab . (innovations-report.com)
iii) The reduction of MMP-9 was found in As2O3-treated groups, more evident in HNE1-LMP1 cells. (scielo.br)
Our study found that iRFP was far superior to the other fluorescent proteins that reportedly help in visualizing the livers of live animals," said Grigory Filonov, Ph.D., a postdoctoral fellow in Dr. Verkhusha''''s laboratory at Einstein, and the first author of the Nature Biotechnology paper. (sciencedaily.com)
We found that the catecholamines reacted readily when enclosed in a dry protein film. (lu.se)

Anatomy49

Organisms36

Diseases1

Chemicals and Drugs46

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes73

Disciplines and Occupations2

Technology, Industry, Agriculture6

Information Science5

Health Care1

Gene19

Human Embryonic Stem3

Jellyfish9

Fluorescence12

Embryonic cells1

Epithelial10

EGFP4

Scientists11

Expression16

Synthesis2

Emits4

Mice10

Nuclei3

Reporter6

Common fluorescent1

Molecules6

Inhibit1

Stem cells6

Antibodies4

Produce a green fluores1

Genetically modified3

Genes5

Localization2

Transplantation5

Eukaryotic3

Populations5

Typically2

Researchers8

Bacteria3

Overexpression3

Receptor4

Recombinant3

Transfection3

Tumor3

Fusion protein6

Marker2

Hippocampus2

19991

Mutagenesis1

Molecular5

Neuronal3

Membrane protein2

Transgenic1

Cellular5

Biology3

Bacterial2

Absorbs1

Flow Cytometry3

Bioluminescent1

Coli5

Heterologous1

Microscopy2

20001

Viability2

Binds2

Receptors1

Indicators1

Proliferation5

Glial3

Tissues and cells1

Degradation1

Roles2

Viral2

Interneurons3

Regulation3

Neurons6

Detect1

Physiology1

Neural1

Anti-apoptotic1

Envelope protein3

Found5