• Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. (cellbiolabs.com)
  • Morphologic alterations of the extracellular matrix in diabetes include thickening of the basement membrane and, as evidenced in the corneal epithelium, a decrease in the area of the basal cell membrane occupied by hemidesmosomes. (nih.gov)
  • These data may indicate altered cell-extracellular matrix interactions in diabetes. (nih.gov)
  • The correct type of extracellular matrix was required, since only a basement membrane, not plastic or a collagen I matrix, lowered the rate of apoptosis. (brighton.ac.uk)
  • Indeed, granule cells from the rostral region of perinatal Gpr56 −/− cerebella show loss of adhesion to extracellular matrix molecules of the pial basement membrane. (jneurosci.org)
  • Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
  • The dynamics of cell-extracellular matrix interactions in normal and pathological situations. (mcgill.ca)
  • The Reinhardt laboratory is interested in components and mechanisms involved in the biogenesis, function and pathological aspects of the extracellular matrix and its interactions with cells. (mcgill.ca)
  • We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells sense normal and pathologically altered extracellular matrix. (mcgill.ca)
  • Dr. Kenneth Yamada's research focuses on discovering novel mechanisms and regulators of cell interactions with the extracellular matrix and their roles in craniofacial development and disease. (nih.gov)
  • Dr. Yamada's group explores how coordination between the three-dimensional (3D) extracellular matrix of connective tissue or basement membranes, integrins, signal transduction, and cytoskeleton mediates or regulates key biological processes - cell adhesion, migration, matrix remodeling, tissue morphogenesis, and cancer cell invasion. (nih.gov)
  • Dr. Yamada's research emphasizes the characterization of the dynamic movements of cells and their extracellular matrix as tissues are remodeled in 3D in real time. (nih.gov)
  • Basement Membrane of Tissue Engineered Extracellular Matrix Scaffolds Modulates Rapid Human Endothelial Cell Recellularization and Promote Quiescent Behavior After Monolayer Formation. (mayo.edu)
  • Basement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold. (mayo.edu)
  • Shklover J, McMasters J, Alfonso-Garcia A, Higuita ML, Panitch A, Marcu L, Griffiths L . Bovine pericardial extracellular matrix niche modulates human aortic endothelial cell phenotype and function. (mayo.edu)
  • extracellular matrix-mediated regulation of cancer stem cells and chemoresistance. (thieme-connect.de)
  • Defining the cells and proteinases responsible for destruction of lung extracellular matrix associated with cigarette smoking will be required for development of appropriate proteinase inhibitors for application in COPD. (atsjournals.org)
  • Epidermolysis bullosa acquisita (EBA) is an autoimmune disease, manifested by autoantibodies that target type VII collagen, the major protein of anchoring fibrils and the one that connects the epithelial basement membrane to the dermis. (medscape.com)
  • The green and black tea extracts, EGCG, and theaflavins decreased the adherence of F. nucleatum to oral epithelial cells and matrix proteins. (nature.com)
  • The series of images in this group have particular historic and cell biologic importance for determining the role of the glomerular basement membrane in urinary filtration, especially the charge properties and size selectivity in retention of proteins in the circulation. (cellimagelibrary.org)
  • By screening for proteins that colocalize with NHR-67 punctae, we identified new regulators of uterine cell fate maintenance: homologs of the transcriptional co-repressor Groucho (UNC-37 and LSY-22), as well as the TCF/LEF homolog POP-1. (elifesciences.org)
  • Further study applying proteomic analysis confirmed that CAFs secrete more proteases, extra-cellular matrix proteins, and proteins that modify the basement membrane compared with NAFs, pointing to a remodeling role for CAFs in invasion. (medindia.net)
  • Hyperglycemia causes glycosylation of glomerular proteins, which may be responsible for mesangial cell proliferation and matrix expansion and vascular endothelial damage. (msdmanuals.com)
  • The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. (cellbiolabs.com)
  • Our CytoSelect™ Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. (cellbiolabs.com)
  • Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells. (cellbiolabs.com)
  • ECM gel can used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells. (sigmaaldrich.com)
  • The biological systems studied include human primary cells migrating in 3D, human tumor cells and tissues, and mouse organ development (salivary glands, lungs, and kidneys). (nih.gov)
  • Activating the nuclear piston mechanism of 3D migration in tumor cells. (nih.gov)
  • Through imaging the tumors and fibroblasts, the researchers then found the smoking gun of cancer-CAF cooperation--long protrusions like puppet-strings that the tumor cells used to communicate with the fibroblasts well before the cancer cells moved to breach the basement membrane. (medindia.net)
  • within a given tumor, cells may be variably arranged in broad sheets, nests, trabeculae, true papillae, or pseudopapillae (see the first 3 images below). (medscape.com)
  • at least focally, tumor cells often have abundant clear cytoplasm, mimicking clear cell RCC. (medscape.com)
  • In Xp11.2 translocation-associated renal cell carcinoma, tumor cells with abundant eosinophilic cytoplasm and high nuclear grade are often arranged in large nests with a delicate, intervening vascular stroma. (medscape.com)
  • The nests of tumor cells in Xp11.2 translocation-associated renal cell carcinomas may become centrally discohesive, giving rise to a pseudoalveolar growth pattern. (medscape.com)
  • Capillaries become inflamed as a result of damage to the basement membrane by antibodies to alpha 3 NC1 domain of type IV collagen. (wikipedia.org)
  • Alport syndrome is a X-linked hereditary nephritis caused by mutations in type IV collagen, leading to a split lamina densa of the glomerular basement membrane. (wikipedia.org)
  • Attachment to a matrix per se was not sufficient for survival, since apoptotic cells were observed when still attached to a collagen I substratum. (brighton.ac.uk)
  • Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells. (unifi.it)
  • Basement membranes were stained with anti-type IV collagen. (nih.gov)
  • The researchers then devised an assay that deployed native basement membrane to separate cancer cells on one side and fibroblasts embedded in collagen on the other. (medindia.net)
  • The COL18A1 gene provides instructions for making a protein that forms collagen XVIII, which is found in the basement membranes of tissues throughout the body. (medlineplus.gov)
  • Collagen XVIII is found in the basement membranes of several parts of the eye, including the vitreous and retina, among other tissues. (medlineplus.gov)
  • Recessive dystrophic epidermolysis bullosa (RDEB) is a severe subtype of epidermolysis bullosa caused by changes in collagen VII with a high risk of early development of cutaneous squamous cell carcinoma (cSCC). (thieme-connect.de)
  • Epidermolysis bullosa acquisita is caused by antibodies targeting type VII collagen, the major component of anchoring fibrils that connect the basement membrane to dermal structures. (medscape.com)
  • [ 3 ] IgG autoantibodies specific for anchoring fibrils (type VII collagen) of the skin basement membrane have a heterogeneous subclass and light-chain composition, and their complement-activating capacities do not correlate with the inflammatory phenotype. (medscape.com)
  • Induction of an active model of autoimmunity against type VII collagen in a hairless mouse strain (SKH1) revealed that regulatory T cells, which have been identified for their ability to inhibit the development of autoimmune diseases, do not inhibit the development of autoantibodies against the self-protein mouse-type VII collagen. (medscape.com)
  • Immunization of type VII collagen in athymic nude SJL mice did not induce an autoimmune response, whereas the repletion of T cells from type VII collagen-immunized wild-type mice to the thymic mice showed autoantibody production and resulted in a blistering disease phenotype, supporting the role of T cells in the induction of epidermolysis bullosa acquisita. (medscape.com)
  • This was followed by repeated selection for increasingly aggressive tumor formation from cells recovered from xenograft tumors in immuno-compromised mice, generating the MCF-10CA1a cell line. (biomedcentral.com)
  • When inoculated subcutaneously into the flanks of immuno-compromised mice, MCF-10AT cells occasionally form tumors, whereas MCF-10CA1a cells invariably form tumors with a shorter latency than MCF-10AT derived tumors. (biomedcentral.com)
  • MCF-10CA1a cells grown in three-dimensional basement membrane culture form large, hyper-proliferative masses, that retain few characteristics of MCF10A acini and more closely resemble tumors. (biomedcentral.com)
  • The basement membrane is also the frontline physical barrier that keeps primary tumors from spreading into the matrix below. (medindia.net)
  • Using human colon cancer cells and primary human fibroblasts isolated from tumors and adjacent normal tissues, Alexandros Glentis and colleagues at the Institut Curie addressed the question of whether the cancer cells or the CAF cells were responsible for the breakdown of the basement membrane that leads to cancer progression. (medindia.net)
  • Another characteristic architectural feature found in many of these tumors is a pseudoalveolar pattern in which cells are arranged in alveolae with central cellular discohesion (see the fourth image below). (medscape.com)
  • Psammoma bodies and scattered xanthoma cells have been described in some tumors. (medscape.com)
  • To determine if the abnormal basement membrane in diabetes has altered epithelial recognition of sites for hemidesmosome formation and if diabetic epithelium can recognize sites for hemidesmosome formation on normal basement membrane, we recombined epithelial sheets and corneal stromas with denuded basement membranes in vitro using diabetic and control corneas. (nih.gov)
  • Numerous goblet cells are intermixed with the epithelium. (medscape.com)
  • We isolated four categories of cells from mammary epithelium of female calves: bromodeoxyuridine label retaining epithelial cells (LREC) from basal (LRECb) and embedded layers (LRECe), and epithelial control cells from basal and embedded layers. (frontiersin.org)
  • The corneal epithelium is composed of stratified squamous epithelial cells on the outer surface of the eye, which acts as a protective barrier and is critical for clear and stable vision. (wjgnet.com)
  • Enteroendocrine cells (EEs) are evolutionarily conserved gastrointestinal secretory cells that show scattered distribution in the intestinal epithelium. (bioone.org)
  • Translocation-associated renal cell carcinomas (RCCs) arise from the proximal tubule epithelium, similar to conventional clear cell and papillary RCCs. (medscape.com)
  • Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
  • Varying degrees of mononuclear cell and eosinophil infiltration, mucus hypersecretion, desquamation of the epithelium, smooth muscle hyperplasia, and airway remodeling are present. (medscape.com)
  • Asthma is a reversible airway obstruction that is characterized by constriction of airway smooth muscle, Abstract hyper secretion of mucus, edema and airway hyper responsiveness (AHR), mucus secretion and thickening of the basement membrane underlying the airway epithelium. (cdc.gov)
  • Modulating the cytokine network in thickening of the basement membrane underlying the respiratory diseases such as asthma, with expected airway epithelium. (cdc.gov)
  • A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
  • Previous molecular characterizations of mammary stem cells (MaSC) have utilized fluorescence-activated cell sorting or in vitro cultivation of cells from enzymatically dissociated tissue to enrich for MaSC. (frontiersin.org)
  • Methodologically, the lab employs a wide spectrum of techniques ranging from in vitro biochemical and biophysical assays, to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo studies with genetically modified mouse models. (mcgill.ca)
  • LMP1-negative cell line HNE1 and LMP1-positive cell line HNE1-LMP1 were used as in vitro model. (scielo.br)
  • A function-blocking anti-b1 integrin antibody doubled the rate of apoptosis in single cells cultured with basement membrane, indicating that integrin-mediated signals contributed to survival. (brighton.ac.uk)
  • Features of tissue morphogenesis were assessed, including proliferation, basement membrane formation, polarization of alpha-6 beta-4 integrin to the basement membrane, formation of cell:cell junctions, and apoptosis for luminal clearance. (biomedcentral.com)
  • The glomerular basement membrane of the kidney is the basal lamina layer of the glomerulus. (wikipedia.org)
  • This dictates cycles of mammary growth, differentiation, lactation, and regression, during which mammary stem cells (MaSC) provide for the lineages of luminal and basal (myoepithelial) epithelial cells in the ducts and alveoli. (frontiersin.org)
  • Duct cells were stained using an antibody to total keratin and a subpopulation of basal duct cells with an antibody to 45/46K keratins. (nih.gov)
  • Basal cell carcinoma, squamous cell carcinoma, and cutaneous melanoma in skin of color patients. (thieme-connect.de)
  • Se localiza en la membrana basal de la lámina lúcida y funciona uniendo las células epiteliales a las membranas basales. (bvsalud.org)
  • Our experiments demonstrate that GPR56 is essential for proper morphogenesis of the rostral cerebellum and suggest that defects caused by loss of GPR56 arise from granule cell abnormalities in this region. (jneurosci.org)
  • Patterned cell and matrix dynamics in branching morphogenesis. (nih.gov)
  • Btbd7 regulates epithelial cell dynamics and branching morphogenesis. (nih.gov)
  • Atherosclerosis starts with dysfunctional changes in the endothelium induced by disturbed shear stress which can lead to endothelial and platelet activation, adhesion of monocytes on the activated endothelium, and differentiation into proinflammatory macrophages, which increase the uptake of oxidized LDL (oxLDL) and turn into foam cells, exacerbating the inflammatory signalling. (hindawi.com)
  • Universal features of breast cancer include a loss of control of cell proliferation and organization, a loss of cell polarity, as well as a loss of cell: cell adhesion and cell: basement membrane adhesion. (biomedcentral.com)
  • Interference RNA-mediated knockdown of GPR56 recapitulates the loss of adhesion seen in knock-outs, and reexpression of GPR56 rescues the adhesion defect in knock-out granule cells. (jneurosci.org)
  • cancer cell growth and spreading, it has also been proposed that GPR56 regulates cell adhesion, but this has remained untested in the nervous system. (jneurosci.org)
  • These results support a novel hypothesis that the cerebellar defects in Gpr56 knock-outs, and potentially in human patients with GPR56 mutations, arise from the disrupted adhesion of developing granule cells to the pial BM. (jneurosci.org)
  • EB differences were restricted to thickening of the bronchial basement membranes in the SCW. (cdc.gov)
  • Conclusion: BT is a treatment option in patients with severe therapy-refractory asthma that downregulates selectively structural abnormalities involved in airway narrowing and bronchial reactivity, particularly ASM, neuroendocrine epithelial cells, and bronchial nerve endings. (lu.se)
  • 5 Waldman A, Schmults C. Cutaneous squamous cell carcinoma. (thieme-connect.de)
  • Anal canal cancer is a rare disease and squamous cell carcinoma is the most common histologic subtype. (bvsalud.org)
  • In three-dimensional reconstituted basement membrane culture, they undergo a well-defined program of proliferation, differentiation, and growth arrest, forming acinar structures that recapitulate many aspects of mammary architecture in vivo . (biomedcentral.com)
  • MCF-10AT cells grown in three-dimensional basement membrane culture form complex multi-acinar structures that produce a basement membrane but undergo delayed cell cycle arrest and have incomplete luminal development. (biomedcentral.com)
  • Basement membranes are thin, sheet-like structures that separate and support cells in these tissues. (medlineplus.gov)
  • Using tapping-mode AFM, which is known as gentle imaging mode and therefore most appropriate for soft biological samples like erythrocytes, it was possible to resolve membrane skeleton structures without major distortions or deformations of the cell surface. (degruyter.com)
  • After a semester at Texas Tech University, USA where I studied atmospheric and geoscience, I worked as upper-secondary teacher for three years before turning back to the university in 2002 to get a master degree in Biophysics, thereby turning from large systems i.e. universe and the atmosphere, towards systems on the micro-nano scale i.e. cells and subcellular structures. (lu.se)
  • My research interest falls into the broad field of regenerative medicine, with emphasis on the way nerve cells and other cell types are affected by small, typically submicron artificial structures. (lu.se)
  • Most sub parts and organelles of a eukaryotic cell are per definition nanostructures, and it is presumed that nano-structures, both natural and artificial should have large impact on various cell functions. (lu.se)
  • Although many biomedical applications may come from these studies, the basic science behind this fascinating interaction between cells and manmade structures is the foundation of my research. (lu.se)
  • In addition to providing biomechanical support to the islets, ECM components are required for β-cell development, proliferation, survival, and proper insulin secretion. (nature.com)
  • The atherosclerotic process is accelerated by a myriad of factors, such as the release of inflammatory chemokines and cytokines, the generation of reactive oxygen species (ROS), growth factors, and the proliferation of vascular smooth muscle cells. (hindawi.com)
  • Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
  • During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)
  • During periods of low mammary proliferation, quiescence of the stem cell population may account for retention of label. (frontiersin.org)
  • Loss of GPR56 does not affect cell proliferation, migration, or neurite outgrowth. (jneurosci.org)
  • Transmission electron micrograph of non-myelinating nerve Schwann cell surrounding nerve fibers from a rat atrium. (cellimagelibrary.org)
  • Immunostained sections were assessed for airway smooth muscle (ASM) area, subepithelial basement membrane thickness, nerve fibers, and epithelial neuroendocrine cells. (lu.se)
  • Tissues and Cells. (lu.se)
  • Epidermolysis bullosa acquisita (EBA) is a chronic autoimmune subepidermal blistering disease of the skin and mucus membranes. (medscape.com)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study. (frontiersin.org)
  • Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine. (cellimagelibrary.org)
  • Image made available by James D. Jamieson and the Department of Cell Biology, Yale U. (cellimagelibrary.org)
  • Besides past service on the Councils of several professional societies, Dr. Yamada is currently an editor of The Journal of Cell Biology and serves other editorial roles at multiple journals. (nih.gov)
  • Dr. Yamada served on the NIH Cell Biology Study Section and currently serves on the NIH Committee on Scientific Conduct and Ethics, NIH Steering Committee Diversity Working Group, and various other NIH committees. (nih.gov)
  • BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
  • During this time, thanks to an interdicipline project in axonal guidance on nanoimprinted patterns, I came in contact with professor Martin Kanje at the Department of Cell and Organism Biology. (lu.se)
  • After one year as research engineer at the department of Cell and Organism Biology I started my PhD with professor Kanje as supervisor. (lu.se)
  • Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in cryosections and characterized the molecular properties of these cells. (frontiersin.org)
  • RNA and DNA in situ hybridization probes for 25 commensal β-HPVs revealed a significant reduction in viral activity and load in human skin cancer compared with the adjacent healthy skin, suggesting a strong immune selection against virus-positive malignant cells. (nature.com)
  • Standing between a cancer cell in situ and the surrounding tissue of fibroblasts and extra-cellular matrix is the basement membrane, a thin sheet of fibers that normally cradles the cells above it. (medindia.net)
  • Researchers at the Institut Curie in Paris now say that they have evidence of a coordinated attack on the basement membrane by cancer cells in situ and CAF cells in the extra-cellular matrix that begins long before the actual trans-location of cancer cells. (medindia.net)
  • An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly tethered to the support. (degruyter.com)
  • abstract = "Apoptosis is an active mechanism of cell death required for normal tissue homeostasis. (brighton.ac.uk)
  • This article shows that laminin α4, Lama4, regulates tip cell (leading endothelial cells during angiogenesis) numbers and vascular density by inducing endothelial Dll4/Notch signalling in vivo . (biolamina.com)
  • At the age of defect onset, GPR56 expression is restricted specifically to developing granule cells in the rostral cerebellum, suggesting that GPR56 regulates properties of these cells. (jneurosci.org)
  • Basement membrane regulates fibronectin organization using sliding focal adhesions driven by a contractile winch. (nih.gov)
  • Local 3D matrix microenvironment regulates cell migration through spatiotemporal dynamics of contractility-dependent adhesions. (nih.gov)
  • MiRNA182 regulates percentage of myeloid and erythroid cells in chronic myeloid leukemia. (ncbs.res.in)
  • Smed-ETS-1 regulates cathepsin+ cell function and epidermal lineage landscape via basement membrane remodeling. (ncbs.res.in)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • Retention of labeled DNA strands may be attributed to the ability of stem cells to retain the parental DNA strand during asymmetric cell division ( Cairns, 1975 ) or to quiescence of the stem cell population such that the DNA label is not diluted by frequent cell divisions ( Klein and Simons, 2011 ). (frontiersin.org)
  • Blood vessels (BVs) are considered an integral component of neural stem cells (NSCs) niches. (elifesciences.org)
  • Neural stem cells (NSCs) serve as a source for new neurons in both the developing and adult brain. (elifesciences.org)
  • In the fruit fly Drosophila, it has been suggested that nearly equal numbers of two subtypes of EEs (Allatostatin A: AstA and Diuretic hormone 31 : Dh31) are alternately produced from the intestinal stem cells in the posterior midgut. (bioone.org)
  • Stem Cells Transl Med. (mayo.edu)
  • Epithelial stem cells in adult mammalian skin are known to maintain epidermal, follicular and sebaceous lineages during homeostasis. (biologists.com)
  • Stem Cells. (qub.ac.uk)
  • The glomerular endothelial cells, the glomerular basement membrane, and the filtration slits between the podocytes perform the filtration function of the glomerulus, separating the blood in the capillaries from the filtrate that forms in Bowman's capsule. (wikipedia.org)
  • Agrin, a protein linked to heparan sulfate basement membrane nephrin Renal corpuscle (glomerulus) showing glomular basement membrane. (wikipedia.org)
  • Basement Membrane" Lote, Christopher J. Principles of Renal Physiology, 5th edition. (wikipedia.org)
  • Therefore, the main aim of this study was to assess the predictive yield of urinary isomorphic red blood cells for disease severity and renal outcomes in patients with ANCA-MPO associated vasculitis. (springer.com)
  • Myeloperoxidase-anti-neutrophil cytoplasmic antibody associated vasculitis patients with predominant urinary isomorphic red blood cells at diagnosis had more severe clinical manifestations and a higher risk of poor renal outcomes. (springer.com)
  • Translocation carcinoma is a renal cell carcinoma (RCC) subtype that harbors a translocation involving a member of the microphthalmia transcription factor gene family. (medscape.com)
  • Xp11.2 translocation-associated renal cell carcinomas are generally cortical or subcapsular, well-circumscribed lesions with yellow-tan, variegated cut surfaces. (medscape.com)
  • In Xp11.2 translocation-associated renal cell carcinoma (RCC), cells typically have voluminous cytoplasm, which can range from eosinophilic and granular to clear. (medscape.com)
  • Xp11.2 translocation-associated renal cell carcinomas (RCCs) may have well-developed papillae, mimicking papillary RCCs. (medscape.com)
  • The results demonstrated that myoepithelial cells are relatively rare in the majority of pleomorphic adenomas and that many of the cells which have been classically described as myoepithelial in routine histological preparations do not clearly show this type of differentiation. (nih.gov)
  • The glomerular basement membrane classically becomes thickened. (msdmanuals.com)
  • CytoSelect™ Cell Invasion Assay Principle. (cellbiolabs.com)
  • Effects of Cytochalasin D on Invading Cells using the CytoSelect™ 24-Well Cell Invasion Assay. (cellbiolabs.com)
  • The vascular BM surrounds the islet capillary network and directly contacts mouse endocrine cells. (nature.com)
  • Thus, human endocrine cells are not in direct contact with the vascular BM components but with the invaginated peri-islet membrane. (nature.com)
  • Li C, Alfonso-Garcia A, McMasters J, Bec J, Weyers B, Uyesaka L, Griffiths L , Panitch A, Marcu L. Simultaneous intraluminal imaging of tissue autofluorescence and eGFP-labeled cells in engineered vascular grafts inside a bioreactor. (mayo.edu)
  • Acute and chronic inflammatory cells are found within these fronds. (medscape.com)
  • Numerous goblet cells are seen along with acute inflammatory cells. (medscape.com)
  • Other immune and inflammatory cells including neutrophils, T lymphocytes, eosinophils, and mast cells are likely also to contribute to proteolytic injury. (atsjournals.org)
  • Resident cells within the lung such as fibroblasts and alveolar type II cells may be induced by cigarette smoke to synthesize chemokines attracting and potentially activating inflammatory cells. (atsjournals.org)
  • Most likely, complex interaction between resident and immune/inflammatory cells results in release of proteolytic enzymes capable of destroying lung tissue and leading to emphysema. (atsjournals.org)
  • Endobronchial (EB) biopsy and bronchoalveolar lavage (BAL) were performed on all study subjects and inflammatory cells were counted. (cdc.gov)
  • IMs are found in the space between the tissue stroma cells and contain, among other components, fibrillar collagens and fibronectin. (nature.com)
  • Invagination into the underlying stroma instead of the exophytic growth pattern is exhibited by squamous cell or limbal papillomas, whereas some lesions exhibit a mixture of exophytic and endophytic growth patterns. (medscape.com)
  • The lesions are composed of lobules of epithelial cells extending down into the stroma. (medscape.com)
  • Scanning electron and atomic force microscopy was used for the first time to view the maturation of the severe acute respiratory syndrome-associated coronavirus at the cell surface. (cdc.gov)
  • The aim of this study was to use scanning electron and atomic force microscopes to investigate changes in the surface topography of SARS-CoV-infected cells at late infection. (cdc.gov)
  • Electron micrograph of an unmyelinated nerve Schwann cell from a rat tongue. (cellimagelibrary.org)
  • Electron micrograph of a nerve from the mesentery of a rat shows groups of unmyelinated axons wrapped by deeply invaginating Schwann cells in cross section. (cellimagelibrary.org)
  • This electron micrograph shows a cross section through an axon, its myelin wrap, and the associated myelin-producing Schwann cell from the the cochlear nerve of the cat. (cellimagelibrary.org)
  • In co-culture experiments on coated trans-filters, both NAFs and CAFs induced migration and invasion of HT29, which are intrinsically noninvasive colon cancer cells. (medindia.net)
  • Then the survival cells were collected to investigate their potential of colony formation, attachment, invasion, and migration. (scielo.br)
  • Confocal micrograph showing nerve cells growing along fibers (purple) made from a specially modified silk that is similar to that made by spiders and silkworms. (cellimagelibrary.org)
  • We propose that basement membrane provides a survival stimulus for epithelial cells in vivo, and that loss of interaction with this type of matrix acts as a control point for cell deletions that occur at specific times during development, such as in mammary gland involution. (brighton.ac.uk)
  • These approaches result in the loss of all histological information pertaining to the in vivo locale of MaSC and progenitor cells. (frontiersin.org)
  • Here, we investigated this dichotomy in vivo by examining two cell types in the developing Caenorhabditis elegans somatic gonad that derive from equipotent progenitors, but exhibit distinct cell behaviors: the post-mitotic, invasive anchor cell and the neighboring proliferative, non-invasive ventral uterine (VU) cells. (elifesciences.org)
  • We propose a model in which association of NHR-67 with the Groucho/TCF complex suppresses the default invasive state in non-invasive cells, which complements transcriptional regulation to add robustness to the proliferative-invasive cellular switch in vivo . (elifesciences.org)
  • We further found that the pericytic laminin isoforms differentially regulate mouse β-cells. (nature.com)
  • In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
  • Here, we have identified a previously undescribed population of epidermal progenitors that reside in the touch domes of hairy skin, termed touch dome progenitor cells (TDPCs). (biologists.com)
  • TDPCs exhibit bipotent progenitor behavior as they give rise to both squamous and neuroendocrine epidermal lineages, whereas the remainder of the α6 + Sca1 + CD200 - epidermis does not give rise to Merkel cells. (biologists.com)
  • Neural Progenitor Cells Derived from XCL-1 DCXp-GFP are a neuronal lineage reporter cell line that can be used in neuronal differentiation and drug screening. (atcc.org)
  • Here we report on the growth and differentiation properties of these three matched cell lines in three-dimensional basement membrane culture. (biomedcentral.com)
  • whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
  • In this respect, urinary isomorphic red blood cells could be viewed as a promising biomarker of ANCA_MPO vasculitis severity and progression. (springer.com)
  • The matched series of normal MCF-10A, pre-malignant MCF-10AT, and malignant MCF-10CA1a cells offers a unique opportunity to study the mechanisms of malignant progression both in a three-dimensional microenvironment and in the same cell background. (biomedcentral.com)
  • A growing body of evidence suggests that cell cycle progression and invasion through a basement membrane are mutually exclusive cellular behaviors in both development and disease states (reviewed in Kohrman and Matus, 2017 ). (elifesciences.org)
  • PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel. (sigmaaldrich.com)
  • Here we show that T cell immunity against commensal papillomaviruses suppresses skin cancer in immunocompetent hosts, and the loss of this immunity-rather than the oncogenic effect of HPVs-causes the markedly increased risk of skin cancer in immunosuppressed patients. (nature.com)
  • Fig. 3: Reduced β-HPV transcripts in skin cancer cells and presence of β-HPV-specific CD8 + T cells in healthy human skin indicates a selective pressure by antiviral immunity against malignant cells with active HPV. (nature.com)
  • Durotaxis by human cancer cells. (nih.gov)
  • Perforating the basement membrane is a cancer cell's first move toward invasion, but how? (medindia.net)
  • do cancer cells invade the basement membrane or do some fibroblasts help invading cancers? (medindia.net)
  • They found that only CAFs were able to stimulate invasion of cancer cells. (medindia.net)
  • The researchers are currently testing a role of CAF-derived molecules in basement membrane remodeling to further dissect the secret alliance between cancer cells and CAFs in basement membrane invasion. (medindia.net)
  • New Therapeutic Procedure To Eliminate Cancer Cells Infiltrated In Brain. (usherbrooke.ca)
  • Then, can it repress the cancer cells' metastasis potential? (scielo.br)
  • arations verified the presence of cancer cells in all samples. (lu.se)
  • While the observation that NHR-67 forms punctae associated with transcriptional repressors in non-invasive cells is intriguing, the work does not yet established a clear link between the formation and dissolution of NHR-67 condensates with the activation of downstream genes that NHR-67 is actively repressing. (elifesciences.org)
  • To download a certificate of analysis for Neural Progenitor Cells Derived from XCL-1 DCXp-GFP ( ACS-5005 ), enter the lot number exactly as it appears on your product label or packing slip. (atcc.org)
  • The certificate of analysis for that lot of Neural Progenitor Cells Derived from XCL-1 DCXp-GFP ( ACS-5005 ) is not currently available online. (atcc.org)
  • MaSC/progenitor cells were identified based on their ability to retain bromodeoxyuridine for an extended period. (frontiersin.org)
  • HT-1080 and NIH3T3 cells (negative control) were seeded at 300,000 cells/well and allowed to invade toward 10% FBS for 24 hrs in the presence or absence of 2µM Cytochalasin D. Invasive cells, on the bottom of the invasion membrane, were stained (above) and then quantified at OD 560 nm after extraction using a standard plate reader (not shown). (cellbiolabs.com)
  • The tea extracts and components displayed various degrees of antibacterial activity that may involve damage to the bacterial cell membrane and the chelation of iron. (nature.com)
  • Previous studies have mainly focused on urinary dysmorphic red blood cells and few have reported the clinical significance of isomorphic urinary red blood cells. (springer.com)
  • A total of 191 patients with ANCA-MPO associated vasculitis with hematuria were retrospectively selected and were divided into two groups (with isomorphic red blood cells versus dysmorphic red blood cells) according to the percentage of isomorphic red blood cells on urinary sediment analysis. (springer.com)
  • Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. (cellbiolabs.com)
  • After 24-48 hours, invasive cells move through the matrix and adhere to the bottom membrane of the insert. (cellbiolabs.com)
  • Non-invasive cells are then removed from the upper chamber, and invasive cells can be either stained and counted using a light microscope or quantified after extraction using a colorimetric or fluorometric plate reader. (cellbiolabs.com)
  • Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. (cellbiolabs.com)
  • How cells switch between proliferative and invasive states is not well understood. (elifesciences.org)
  • We show that the fates of these cells post-specification are more plastic than previously appreciated and that levels of NHR-67 are important for discriminating between invasive and proliferative behavior. (elifesciences.org)
  • During development of the hermaphroditic reproductive system, the proximal granddaughters of the Z1 and Z4 somatic gonad progenitors, Z1.pp and Z4.aa, give rise to four cells that will adopt one of two cellular fates: a proliferative ventral uterine (VU) cell or the terminally differentiated, invasive anchor cell (AC) ( Figure 1A ) ( Kimble and Hirsh, 1979 ). (elifesciences.org)
  • This results in a tight but non-invasive attachment of the cells. (degruyter.com)