• vitro
  • A cell-free system is an in vitro tool widely used to study biological reactions that happen within cells apart from a full cell system, thus reducing the complex interactions typically found when working in a whole cell. (wikipedia.org)
  • This study provides an extraordinary visualization of cleavage furrow and aster formation and, for the first time, reconstitutes this process in an in vitro experimental system. (xenbase.org)
  • Systems biocatalysis is an approach consisting of organizing enzymes in vitro to generate an artificial metabolism for synthetic purposes following the identification of a reaction sequence catalysed by specific enzymes, construction of a controllable metabolic pathway and process intensification for the effective production of the products. (teknoscienze.com)
  • The Y-Chromosome Deletion Detection System from Promega also carries the CE Mark for use as an in vitro diagnostic device in the European Union. (wikipedia.org)
  • amino acids
  • Subsequent studies showed that (i) every cell has multiple species of tRNA, each of which is associated with a single specific amino acid, (ii) that there are a matching set of enzymes responsible for linking tRNAs with the correct amino acids, and (iii) that tRNA anticodon sequences form a specific decoding interaction with mRNA codons. (wikipedia.org)
  • mRNA
  • The first direct experimental evidence for the existence of mRNA was provided by such a hemoglobin synthesizing system. (wikipedia.org)
  • To test whether their observation of decreased mRNA levels was a result of mRNA being targeted directly (as suggested by data from other systems), Drosophila S2 cells were transfected with either Drosophila cyclin E dsRNAs or lacZ dsRNAs and then incubated with synthetic mRNAs for cyclin E or lacZ. (wikipedia.org)
  • In a twist, there are let-7-binding sites in MYC 3' untranslated region(UTR) according to bioinformatic analysis, and let-7 overexpression in cell culture decreased MYC mRNA levels. (wikipedia.org)
  • Xenopus
  • In a new paper appearing in Science (10 October 2014, Science 346 (6206):244-247 ), Phuong Nguyen and Aaron Groen, along with their colleagues at Harvard Medical School (Boston, MA), have reconstituted the organization of cytokinesis signaling outside living cells, using a the cell-free Xenopus system. (xenbase.org)
  • genes
  • 1992: Roeder's laboratory demonstrates that coactivators can be ubiquitous, monitoring many genes in a variety of cells, or specific to one particular cell type. (wikipedia.org)
  • Given that the expression levels of let-7 members are significantly low in human cancers and cancer stem cells, the major function of let-7 genes may be to promote terminal differentiation in development and tumor suppression. (wikipedia.org)
  • As one of the four genes involved in induced pluripotent stem (iPS) cells reprogramming, LIN28 expression is reciprocal to that of mature let-7. (wikipedia.org)
  • Let-7 has been demonstrated to be a direct regulator of RAS expression in human cells All the three RAS genes in human, K-, N-, and H-, have the predicted let-7 binding sequences in their 3'UTRs. (wikipedia.org)
  • apoptosis
  • Publications] Yokoyama, Y.: 'Enhancement of apoptosis in developing chick neural retina cells by basic fibroblast growth factor' Jounal of Neurochemistry. (nii.ac.jp)
  • Publications] Yokoyama, Y.: 'CPP32 activation during dolichyl phosphate-induced apoptosis in U937 leukemia cells' FEBS Letters. (nii.ac.jp)
  • ribosomes
  • The remains are the necessary cell machinery including ribosomes, aminoacyl-tRNA synthetases, translation initiation and elongation factors, nucleases, etc. (wikipedia.org)
  • coli
  • Following the work of Alfred Tissieres and after a few failed attempts, they created a stable system by rupturing E. coli bacteria cells and releasing the contents of the cytoplasm. (wikipedia.org)
  • biology
  • These systems have enabled cell-free synthetic biology to emerge, providing control over what reaction is being examined, as well as its yield, and lessening the considerations otherwise invoked when working with more sensitive live cells. (wikipedia.org)
  • We've just published our special issue focussing on plant cell biology - check out the table of contents here , and a selection of the issue's highlights below. (biologists.org)
  • In this interview , our Special Issue's guest editor Jenny Russinova discusses her excitement for plant cell biology and what she hopes to achieve with the issue. (biologists.org)
  • We are now accepting submissions for our upcoming special issue on 'Reconstituting cell biology', guest edited by Manuel Théry. (biologists.org)
  • Promega offers a range of products for cell biology and drug discovery, many of which are built upon bioluminescence technology. (wikipedia.org)
  • gene expression
  • As the exclusive licensed distributor of tetracycline-controlled gene expression systems, only Clontech is certified to offer FBS that can ensure optimum induction with all tetracycline inducible gene expression systems. (clontech.com)
  • Because Clontech's Tet-On and Tet-Off Systems are extremely sensitive, even "antibiotic-free" fetal bovine serum that has no negative effect on cell growth may still exhibit enough activity to alter Tet-regulated gene expression. (clontech.com)
  • In melanocytic cells GM2A gene expression may be regulated by MITF. (wikipedia.org)
  • Drosophila S2 cells were transfected with a lacZ expression vector to quantify gene expression with β-galactosidase activity. (wikipedia.org)
  • replication
  • Immunobloting exper iments using antibody against the 87 kDa polypeptide showed that behavior of DUF during a cell cycle or embryogenesis co-relates with DNA replication. (nii.ac.jp)
  • Immunodepletion of DUF from the DNA synthetic system lead to inhibition of intiation of DNA replication. (nii.ac.jp)
  • Drosophila
  • Hannon and his colleagues attempted to identify the RNAi mechanisms involved in gene silencing, by dsRNAs, in Drosophila cells. (wikipedia.org)
  • S2 cells were then transfected with Drosophila cyclin E dsRNA. (wikipedia.org)
  • polymerases
  • The portfolio of amplification products includes the GoTaq family of polymerases and buffers and the Plexor quantitative PCR system. (wikipedia.org)
  • 1969-1977: In 1969, as a graduate student at the University of Washington, Roeder discovers that three enzymes, called RNA polymerases, directly copy DNA in animal cells. (wikipedia.org)
  • stable
  • Prion aggregates are extremely stable and accumulate in infected tissue, causing tissue damage and cell death. (wikipedia.org)
  • serum
  • High-quality Tet System Approved Fetal Bovine Serum for cell culture has been functionally tested * to ensure that it permits the full range of tetracycline-regulated induction with well-characterized Tet cell lines. (clontech.com)
  • Other commercial cell culture serum products may contain tetracycline-derived contaminants which can dramatically affect the inducible regulation of Tet Systems. (clontech.com)
  • Testing for the presence of tetracyclines in fetal or calf serum is not equivalent to the highly sensitive functional testing that is performed on Clontech Tet System Approved FBS. (clontech.com)
  • A premium cell culture serum that is collected in the United States. (clontech.com)
  • The only fetal bovine serum that is functionally tested with Clontech's tetracycline inducible expression systems. (clontech.com)
  • genomes
  • The patchy homologies are similar to those found earlier during the analyses of some of the junction sequences in integrated Ad12 genomes in Ad12-induced hamster tumor cell lines. (uni-muenchen.de)
  • In addition to DNA found outside the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA. (wikipedia.org)
  • enzymes
  • The company's portfolio began with products for genomics researchers and now includes cloning systems, luciferase reporters, and amplification products as well as the original restriction and modifying enzymes. (wikipedia.org)
  • transcripts
  • Although the levels of mature let-7 members are undetectable in undifferentiated cells, the primary transcripts and the hairpin precursors of let-7 are present in these cells. (wikipedia.org)
  • stimulates
  • The 8-azido-ATP photolabeling system shows that GSSG stimulates ATP hydrolysis without affecting ATP binding, whereas GSH decreases ATP binding. (bu.edu)
  • synthesis
  • Cell-free biosystems have several advantages suitable in industrial applications: Very high product yields are usually accomplished without the formation of by-products or the synthesis of cell mass. (wikipedia.org)
  • species
  • Publications] Takayanagi, S.: 'Sulfolobus hakonensis sp.nov.,anovel species of acidothermophilic Archaeon' Int.J.System.Bacteriol.46・2. (nii.ac.jp)
  • study
  • In this dissertation an 8-azido-ATP photolabeling system is established to study the ATP binding and hydrolysis properties of ABCB10. (bu.edu)
  • 1977-1979: Roeder develops cell-free systems to better study transcription. (wikipedia.org)
  • Therefore
  • Therefore, the 8-azido-ATP photolabeling system can be utilized to test potential substrates of ABCB10. (bu.edu)
  • genetic
  • citation needed] The company is one of two main suppliers of systems for genetic identification based on DNA analysis using short tandem repeats (STRs). (wikipedia.org)
  • activity
  • The activity promoting cell-free recombination elutes from the Sephacryl S-300 matrix with the shoulder and not the peak fractions of the absorbancy profile. (uni-muenchen.de)
  • resistant
  • The mutants of other let-7 family members have a radio-resistant phenotype in vulval cells, which may be related to their ability to repress RAS. (wikipedia.org)
  • colleagues
  • In their Cell Science at a Glance article and accompanying poster, Staffan Persson and colleagues survey cell wall assembly. (biologists.org)
  • Roeder and colleagues introduce the concept of cell specificity after they demonstrate that the coactivator OCA-B, the first cell-specific coactivator, discovered by Roeder in 1992, is unique to immune system B cells. (wikipedia.org)
  • 2002: Roeder and colleagues show that a single component of the mediator is essential for the formation of fat cells - a finding that may one day contribute to new treatments for diabetes, heart disease, cancer and other conditions in which the fat-making process breaks down. (wikipedia.org)