• As one of the first pioneers of cell lineage, in the 1960s Dr. Sydney Brenner first began observing cell differentiation and succession in the nematode Caenorhabditis elegans. (wikipedia.org)
  • Initial results showed that the nematode was eutelic (each individual experiences the same differentiation pathways), however work by Sulston and Richard Horvitz showed that several cells necessary for reproduction differentiate after hatching. (wikipedia.org)
  • Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage. (ca.gov)
  • We included details on materials and techniques, suggest ways to customize it to your hPSC line of choice, added notes on how to monitor and analyze the cells during differentiation, and indicate what results can be expected. (ca.gov)
  • Here we present Digital Development (http://www.digital-development.org), a database of cell lineage differentiation with curated phenotypes, cell-specific gene functions and a multiscale model. (nih.gov)
  • The database stores data from recent systematic studies of cell lineage differentiation in the C. elegans embryo containing ∼ 200 conserved genes, 1400 perturbed cell lineages and 600,000 digitized single cells. (nih.gov)
  • This information includes lineage differentiation phenotypes, cell-specific gene functions, differentiation landscapes and fate choices, and a multiscale model of lineage differentiation. (nih.gov)
  • The scale, resolution and richness of biological information presented here facilitate exploration of gene-specific and systems-level mechanisms of lineage differentiation in Metazoans. (nih.gov)
  • The concept of cell fate relates to the future identity of a cell, and its daughters, which is obtained via cell differentiation and division. (mdc-berlin.de)
  • Second, altering the MSC state by inducing differentiation into multiple lineages did not affect the exosomal properties or endocytosis but triggered the expression of lineage-specific genes and proteins in vitro and in vivo respectively. (frontiersin.org)
  • Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)
  • Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
  • The origins and basis for these fat deposits are largely unknown, but there is a possibility that the altered mechanical and biological environments lead to dysregulation of MSC/MPC and progression to preferential differentiation towards the adipocyte lineage. (scirp.org)
  • Hart, D. (2014) Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
  • Ando, W., Heard, B., Nakamura, N., Frank, C.B. and Hart, D.A. (2012) Ovine Synovial Membrane-Derived Mescenchymal Progenitor Cells Retain the Phenotype of the Original Tissue That Was Exposed to in Vivo Inflammation: Evidence for a Suppressed Chondrogenic Differentiation Potential of the Cells. (scirp.org)
  • In particular, an excess of Cdx2 over Oct4 determines the trophectoderm lineage whereas an excess of Gata-6 over Nanog determines differentiation into the endoderm lineage. (lu.se)
  • The model also predicts that reprogramming the network from a differentiated state, in particular the endoderm state, into a stem cell state, is best achieved by over-expressing Nanog, rather than by suppression of differentiation genes such as Gata-6. (lu.se)
  • However, we lack information on when and where T-bet is expressed during T cell differentiation and how this impacts T cell differentiation and function. (kcl.ac.uk)
  • This report addresses the issue of optimizing extracellular matrix protein density required to support osteogenic lineage differentiation of mesenchymal stem cells (MSCs) by culturing MSCs on surface-bound density gradients of immobilized collagen type I (COL1) and osteopontin (OPN). (monash.edu)
  • The Runx2 intensity and calcified area increase with increasing COL1 density up to a critical value corresponding to 124.2 ng cm −2 , above which cell attachment and differentiation do not rise further, while this critical value for OPN is 19.0 ng cm −2 . (monash.edu)
  • To further promote differentiation and to increase mechanical signaling, Notch signaling was activated by presenting cells with fibronectin and DLL1 protein. (siu.edu)
  • Australia allowed the other download Human Embryonic and Induced Pluripotent Stem Cells: Lineage Specific Differentiation Protocols to impose software for methods in 1902 and Canada was also system centuries as emotional Goodreads by video until 1922. (idealnaja.pl)
  • Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation. (ku.dk)
  • We also found an unexpectedly dynamic pattern of transcript isoform regulation, suggesting a critical regulatory role during HSC differentiation, and a cell cycle/DNA repair signature associated with multipotency in MPP2 cells. (lu.se)
  • This change in gene activity blocks the maturation (differentiation) of blood cells and leads to the production of abnormal, immature white blood cells called myeloid blasts. (medlineplus.gov)
  • Within the B-cell and T-cell categories, two subdivisions are recognized: precursor neoplasms, which correspond to the earliest stages of differentiation, and more mature differentiated neoplasms. (medscape.com)
  • Cell lineage can be determined by two methods, either through direct observation or through clonal analysis. (wikipedia.org)
  • CRISPR-Cas9 lineage tracing technologies have emerged as a powerful tool for investigating development in single-cell contexts, but exact reconstruction of the underlying clonal relationships in experiment is plagued by data-related complications. (biorxiv.org)
  • These indel mutations are subsequently inherited by future descendants, and the accumulation of these mutations is used to infer the clonal relationships between the observed cells, stratifying them into clades of increasing resolution. (biorxiv.org)
  • These approaches provide limited information on the ontogenies and clonal B cell lineages that expand following Env-immunization. (plos.org)
  • Systemic mastocytosis with associated clonal hematological non-mast cell lineage disease: clinical significance and comparison of chomosomal abnormalities in SM and AHNMD components. (umassmed.edu)
  • An MDS, as defined by the World Health Organization (WHO), is a clonal hematopoietic stem cell disorder resulting in ineffective hematopoiesis that manifests as morphologic dysplasia in hematopoietic cells and peripheral cytopenias. (medscape.com)
  • Lgr5 is a well-established marker of these basal stem cells in the gastrointestinal tract 1 and lineage tracing experiments have demonstrated that Lgr5 + cells give rise to clonal cell populations establishing entire colonic crypts 2 . (nature.com)
  • Many research groups are engaged in using human pluripotent stem cells (hPSCs) to generate surrogate pancreatic beta-cells for transplantation into diabetic patients. (ca.gov)
  • Human induced pluripotent stem cells (hiPS) constitute an appropriate cellular model that can be exploited to mimic and recapitulate brain development. (bmj.com)
  • Bat pluripotent stem cells provide insights into their unique relationship with viruses. (ipscell.com)
  • Bat pluripotent stem cells reveal unusual entanglement between host and viruses , Cell . (ipscell.com)
  • Future studies with human pluripotent stem cells may transduce cells towards mesoderm and finally towards cardiac fate. (siu.edu)
  • These links are download Human Embryonic and Induced Pluripotent Stem Cells: year, ideas, time drag-and-drop, sentence of the market, etc. (idealnaja.pl)
  • Similar uncertainties do Free Two-Day Shipping, Free responsible or academic download Human Embryonic and Induced Pluripotent Stem Cells: Lineage Specific to need robotics, Prime Video, Prime Music, and more. (idealnaja.pl)
  • Such cells from different sources can proliferate and differentiate into different lineages (e.g. osteogenic, chondrogenic and adipogenic) after suitable stimulation. (scirp.org)
  • The computational model provides a mechanistic understanding of how different lineages arise from the dynamics of the underlying regulatory network. (lu.se)
  • In multicellular organisms, the lineage history and spatial organization of cells both play pivotal roles in cell fate determination during development, homeostasis, and disease. (caltech.edu)
  • A study in Nature suggests epitope editing in donor stem cells prior to bone marrow transplants can stave off toxicity when targeting acute myeloid leukemia with immunotherapy. (genomeweb.com)
  • Myelodysplastic syndrome (MDS) with single lineage dysplasia (SLD), formerly known as refractory cytopenia with unilineage dysplasia (RCUD), is a category of MDS characterized by morphologic dysplasia in at least 10% of the early cells of a single myeloid lineage (red blood cells, white blodd cells, or megakaryocytes) with associated peripheral blood cytopenia. (medscape.com)
  • Myelodysplastic syndrome (MDS) with single lineage dysplasia (SLD) is characterized by morphologic dysplasia of a single myeloid lineage with associated one or two peripheral blood cytopenia(s). (medscape.com)
  • In the bone marrow, at least 10% of a given myeloid lineage should be affected. (medscape.com)
  • While t(8;21) is important for leukemia development, a mutation in one or more additional genes is typically needed for the myeloid blasts to develop into cancerous leukemia cells. (medlineplus.gov)
  • Transcription factor RUNX1 promotes survival of acute myeloid leukemia cells. (medlineplus.gov)
  • abstract = "T-bet is the lineage-specifying transcription factor for CD4 + T H1 cells. (kcl.ac.uk)
  • abstract = 'Mitochondrial dysfunction has been demonstrated to result in premature aging due to its effects on stem cells. (ku.dk)
  • Recent insights obtained from single-cell genomic and integrative lineage-tracing approaches have further aided to identify molecular features predictive of cell fate. (mdc-berlin.de)
  • Epithelial stem cells in adult mammalian skin are known to maintain epidermal, follicular and sebaceous lineages during homeostasis. (biologists.com)
  • We have shown that in the stomach, stem cell homeostasis is regulated by Wnt and Rspo secreted by stromal myofibroblasts 11 . (nature.com)
  • We demonstrate that while Rspo3 from myofibroblasts maintains colonic Lgr5 + cells during homeostasis, during injury its main function is not to maintain Lgr5 + cells but to interact with more differentiated cells that express Lgr4 but not Lgr5 and are able to regain expression of Wnt target genes and generate new crypts. (nature.com)
  • This type of lineage can be studied by marking a cell (with fluorescent molecules or other traceable markers) and following its progeny after cell division. (wikipedia.org)
  • Current lineage tracking approaches rely on the progressive accumulation of either naturally-occurring somatic mutations or experimentally introduced markers. (caltech.edu)
  • We demonstrate that T-bet is expressed in a subset of CD4 + T cells that have naïve cell surface markers and transcriptional profile and that this novel cell population is phenotypically and functionally distinct from previously described populations of naïve and memory CD4 + T cells. (kcl.ac.uk)
  • These results demonstrate that lineage-specifying factors can polarize T cells in the absence of canonical markers of T cell activation and that this has an impact on the subsequent T-helper response. (kcl.ac.uk)
  • Here we identify a vertebral skeletal stem cell (vSSC) that co-expresses ZIC1 and PAX1 together with additional cell surface markers. (bvsalud.org)
  • DESIGN: Proliferation, mitosis and the expression of progenitor lineage markers were assessed in normal squamous oesophagus from 10 patients by immunofluorescence on 3D epithelial whole mounts. (who.int)
  • Cells expressing differential levels of epithelial and progenitor markers were isolated using flow cytometry sorting and characterised by qPCR and IF. (who.int)
  • This causes the cell lineage and cell fate to be highly correlated. (wikipedia.org)
  • After mapping various sections of the C. elegans' cell lineage, Dr. Brenner and his associates were able to piece together the first complete and reproducible fate map of cell lineage. (wikipedia.org)
  • This high correlation between cell lineage and cell fate was thought to be determined by segregating factors within the dividing cells. (wikipedia.org)
  • Perhaps the most popular method of cell fate mapping in the genetic era is through site-specific recombination mediated by the Cre-Lox or FLP-FRT systems. (wikipedia.org)
  • With the system, researchers could investigate the function of their favorite gene in determining cell fate by designing a genetic model where within a cell one recombination event is designed for manipulating the gene of interest and the other recombination event is designed for activating a reporter gene. (wikipedia.org)
  • Understanding, predicting, and manipulating cell fate has been a long-sought goal of developmental and regenerative biology. (mdc-berlin.de)
  • In this perspective, we discuss these approaches with a focus on theoretical concepts and future directions of the field to dissect molecular mechanisms underlying cell fate. (mdc-berlin.de)
  • We evaluated the reconstruction accuracy of our system in mouse embryonic stem (mES) cells and disentangled the relative contribution of lineage and space to cell fate determination in Drosophila brain development, establishing the foundation for an expandable synthetic microscopy-readable system. (caltech.edu)
  • In this thesis, Chapter 1 introduces the importance of cell lineage and spatial organization to cell fate determination, and includes a brief history of the existing technologies of the lineage tracking field. (caltech.edu)
  • To address this, we traced the ontogeny of T-bet-expressing cells using a fluorescent fate-mapping mouse line. (kcl.ac.uk)
  • An analysis of cell lineage and fate mapping and an assessment of methods. (wikidata.org)
  • Thus, we find that endogenous Rspo3 signaling is a critical determinant of cellular fate within the crypt and stimulates rapid recruitment of differentiated cells for epithelial wound healing and crypt regeneration through induction of Wnt signaling. (nature.com)
  • Yet asexuality - production of progeny through proliferation of mitotic cell-lineages - is found in over one half of all eukaryotic phyla, and is likely to contribute to adaptive changes, as suggested by recent evidence from both animals and plants. (edu.au)
  • Second, the rapid proliferation of these cells, along with a reduction in their ability to undergo programmed cell death (apoptosis), results in their accumulation in the bone marrow, the blood, and, frequently, the spleen and liver. (medscape.com)
  • Here, we have identified a previously undescribed population of epidermal progenitors that reside in the touch domes of hairy skin, termed touch dome progenitor cells (TDPCs). (biologists.com)
  • This has been studied mainly in the small intestine, where different cell types such as enterocyte progenitors 4 , more differentiated secretory Bmi1 + cells 3 , or even fully differentiated Paneth cells can repopulate crypts upon injury. (nature.com)
  • Quadrivalent influenza vaccines containing both lineages may improve the effectiveness of influenza vaccine programmes in the future. (who.int)
  • Below, we outline a method that is based on published protocols as well as our own experience by which one can differentiate hPSCs along the pancreatic lineage to generate insulin-producing beta-cell-like cells. (ca.gov)
  • These colors are fluorescent and marked on the proteins by administering injections to trace such cells. (wikipedia.org)
  • Phylogenetic trees are routinely constructed to describe the developmental relationships within sets of extant taxa such as different organisms, proteins, or single cells. (biorxiv.org)
  • Rspo proteins are secreted and can stabilize the effects of Wnt ligands by preventing ubiquitination and turnover of the Wnt receptor frizzled 9 , thereby dictating the size of the Lgr5 + stem cell pool by regulating self-renewal of Lgr5 + cells 10 . (nature.com)
  • permeability barrier surrounding cells or organelles and consists of a phospholipids bilayer, together with associated membrane proteins. (wormclassroom.org)
  • array of microtubules and associated proteins that forms between the opposite poles of a eukaryotic cell during mitosis and serves to move the duplicated chromosomes apart. (wormclassroom.org)
  • CCIIV for the cell culture based inactivated vaccine, AIV for the adjuvated inactivated influenza vaccine. (cdc.gov)
  • The two forms were equally immunogenic, but only the latter elicited neutralizing antibodies by stimulating a more restricted expansion of B cells to a narrower set of IGH/IGK/IGL-V genes that represented a small fraction (0.003-0.02%) of total B cells. (plos.org)
  • 140). ZIKV infection enhances the expression of immune-related genes in early stage MSCs while increasing cell cycle genes in late stage MSCs. (elsevierpure.com)
  • The RUNX1 protein turns on (activates) genes that help control the development of blood cells (hematopoiesis). (medlineplus.gov)
  • Finally, a mis-tuned "Cas9 editing rate" - the rate at which Cas9 induces heritable mutations used for lineage tracing - can lead to scenarios where there is a lack of mutation information sufficient for discerning relationships between cells. (biorxiv.org)
  • To this purpose, we use extensive simulations (informed by real experimental data) to quantify how different factors (recorder design, mutation rates, cell lineage depth, etc.) can undermine the accuracy of lineage reconstruction. (irbbarcelona.org)
  • In 2005, researchers discovered a mutation in the Janus Tyrosine Kinase 2 gene (JAK2 (V617F)), which plays a pivotal role in the regulation of blood cell production (Levine et al. (cdc.gov)
  • the JAK2 mutation, the presence of the mutation has become an important diagnostic criterion for identifying patients with PV and for reducing the potential for misdiagnosis of persons with elevated red blood cell counts. (cdc.gov)
  • Adaptive changes in mitotic lineages can be reconciled with contemporary evolutionary thought by fully abandoning the weismannian concept of individuality. (edu.au)
  • Patients with AML often have decreased neutrophil levels despite an increased total white blood cell (WBC) count. (medscape.com)
  • Transgenic reporter mouse embryonic stem cells were used in this study. (siu.edu)
  • To promote adhesion of mouse embryonic stem cells and to initiate integrin-based mechanical signaling, the extracellular matrix protein fibronectin was used. (siu.edu)
  • In the presence of fibronectin and a small molecule called leukemia inhibitory factor (LIF), which is known to maintain pluripotency and self-renewal, mouse embryonic stem cells responded by exhibiting expressions of both GFP and DsRed. (siu.edu)
  • By utilizing the Cre-Lox or FLP-FRT recombination systems, a reporter gene (usually encoding a fluorescent protein) is activated and permanently labels the cell of interest and its offspring cells, thus the name cell lineage tracing. (wikipedia.org)
  • The combination of the two offers the opportunity to map gene functions and gene networks in vivo at single-cell resolution using cell tracking and quantification of cellular phenotypes. (nih.gov)
  • The reporter cell line shows the cellular endogenous activity of pluripotency gene Oct3/4 with green fluorescent protein (GFP) while also reporting mesoderm-specific gene, Brachyury, activity with DsRed fluorescent protein. (siu.edu)
  • process usually involving changes in gene expression by which a precursor cell becomes a distinct specialized cell type. (wormclassroom.org)
  • Set apart from other CD4+ populations by its distinct transcriptional profile, unique gene regulatory networks, and non-overlapping TCR repertoire, the cells contributed to pathological impairment in the GI tract. (astct.org)
  • The viral S gene is important as it codes for the Spike protein which is the molecule that makes contact with, and allows entry of the virus into susceptible host cells, causing infection. (who.int)
  • Some mutations in the S gene may lead to changes in the spike protein which result in inhibition of contact and entry of the virus into human cells, however in the case of the VOC, they contain mutations in the S gene that enhance the process of contact and entry into human cells, increasing transmissibility of the virus. (who.int)
  • Together, our results indicate that vSSCs are distinct from other skeletal stem cells and mediate the unique physiology and pathology of vertebrae , including contributing to the high rate of vertebral metastasis . (bvsalud.org)
  • Mesenchymal stem cells (MSCs) are multipotent cells with regenerative and immunomodulatory properties. (frontiersin.org)
  • Human mesenchymal stem cells (HMSCs) are multipotent somatic stem cells that can be isolated from a variety of tissues such as the bone marrow, adipose tissue, and dental pulp. (frontiersin.org)
  • Mesenchymal stem/progenitor cells (MSC/MPC) are found in many tissues and fluids including bone marrow, adipose tissues, muscle, synovial membranes, synovial fluid, and blood. (scirp.org)
  • Hart, D.A. (2014) Why Mesenchymal Stem Cell/Progenitor Cell Heterogeneity in Specific Environments? (scirp.org)
  • Wu, Y. and Zhao, R.C. (2012) The Role of Chemokines in Mesenchymal Stem Cell Homing to the Myocardium. (scirp.org)
  • Li, L. and Jiang, J. (2011) Regulatory Factors of Mesenchymal Stem Cell Migration into Injured Tissues and Their Signal Transduction Mechanisms. (scirp.org)
  • Fehrer, C. and Lepperdinger, G. (2005) Mesenchymal Stem Cell Aging. (scirp.org)
  • Batsali, A.K., Kastrinaki, M.C., Papadaki, H.A. and Pontikoglou, C. (2013) Mesenchymal Stem Cells Derived from Wharton's Jelly of the Umbilical Cord: Biological Properties and Emerging Clinical Applications. (scirp.org)
  • It's funny that mesenchymal cells or MSCs are often talked about the most in terms of having immune evading potential, but MSC products often are partially differentiated and extremely heterogeneous. (ipscell.com)
  • Overall, this work provides a first theoretical analysis of phylogenetic reconstruction in the CRISPR-Cas9 lineage tracing technology. (biorxiv.org)
  • Recent progress in CRISPR-Cas9 based lineage tracing technologies now enables the inference of cellular lineage relationships in more complex organisms where visual observation is not possible. (biorxiv.org)
  • Despite the many advantages of CRISPR-Cas9 lineage tracing systems, an outstanding goal is develop methods to accurately infer the underlying developmental process and to determine under what experimental conditions the problem is tractable. (biorxiv.org)
  • Furthermore, some fluorescent reporters have such an extremely low recombination threshold that they may label cell populations at undesired time-points in the absence of induction. (wikipedia.org)
  • We identify two populations of cells with active Wnt signaling: highly proliferative Lgr5 + /Axin2 + cells, as well as secretory Lgr5 − /Axin2 + cells. (nature.com)
  • In this study, we present integrated quantitative proteome, transcriptome, and methylome analyses of hematopoietic stem cells (HSCs) and four multipotent progenitor (MPP) populations. (lu.se)
  • Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)
  • More recently, researchers have begun using synthetic biology approaches and the CRISPR/Cas9 system to engineer new genetic systems that enable cells to autonomously record lineage information in their own genome. (wikipedia.org)
  • 2010. Genome-wide MyoD binding in skeletal muscle cells: a potential for broad cellular reprogramming. (fhcrc.org)
  • Cell lineage denotes the developmental history of a tissue or organ from the fertilized embryo. (wikipedia.org)
  • The protocol, which spans five distinct stages, is an attempt to recapitulate the derivation of pancreatic beta-cells in vitro as they form in the developing embryo. (ca.gov)
  • one of two haploid nuclei in a newly fertilized embryo (one maternal and one paternal) (Adapted from The Cell A Molecular Approach. (wormclassroom.org)
  • The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo. (bvsalud.org)
  • Through single-cell RNA sequencing analysis, scientists have identified a developmentally distinct subset of T cells in the colon that are implicated in graft-versus-host disease (GVHD). (astct.org)
  • 1 Currently, four antigenically distinct groups of influenza viruses have been identified as the cause of human infection, including two subtypes of influenza A (A/H1N1 and A/H3N2) and two lineages of influenza B. The two influenza B lineages are represented by the reference strains B/Victoria/2/87 and B/Yamagata/16/88. (who.int)
  • Stem Cell Reviews, 8, 243-250. (scirp.org)
  • Recent studies have associated the transcription factors, Oct4, Sox2 and Nanog as parts of a self-regulating network which is responsible for maintaining embryonic stem cell properties -- self renewal and pluripotency. (lu.se)
  • It provides a framework to explore strategies of reprogramming a cell from a differentiated state to a stem cell state through directed perturbations. (lu.se)
  • Such an approach is highly relevant to regenerative medicine since it allows for a rapid search over the host of possibilities for reprogramming to a stem cell state. (lu.se)
  • As you can see in that post, I didn't find convincing data to either back up the claimed stem cell connection. (ipscell.com)
  • If you haven't already, please also take a look at our stem cell YouTube channel and subscribe. (ipscell.com)
  • I wrote about the preprint for this bat stem cell work earlier. (ipscell.com)
  • On the other hand, any allogeneic cell, even a stem cell, is going to be recognized in some way as foreign. (ipscell.com)
  • Signals that regulate epithelial regeneration upon stem cell injury are largely unknown. (nature.com)
  • Expression of Lgr5 is controlled by Wnt signaling, which plays a critical role for stem cell turnover in the gastrointestinal tract 2 . (nature.com)
  • In this issue of Cell Stem Cell, Yang et al. (newswise.com)
  • A vertebral skeletal stem cell lineage driving metastasis. (bvsalud.org)
  • Three is for trivalent, which have H1N1, H3N2, and 1 influenza B virus from 1 B virus lineage. (cdc.gov)
  • In addition, mutual antagonism between two of these and other master regulators have been shown to regulate lineage determination. (lu.se)
  • The colonic epithelial turnover is driven by crypt-base stem cells that express the R-spondin receptor Lgr5. (nature.com)
  • Chemical injury induced by DSS leads to a loss of both Lgr5 + cells and Axin2 + cells and epithelial regeneration is driven by Axin2 − cells, including differentiated Krt20 + surface enterocytes. (nature.com)
  • We demonstrate that Rspo3 is essential for epithelial repair via induction of Wnt signaling in differentiated cells. (nature.com)
  • The majority of epithelial cells are therefore short-lived, in contrast to a limited number of long-lived stem cells in the base of the crypts. (nature.com)
  • While Lgr5 + cells are long-lived and act as stem cells during homeostatic conditions, experimental models have demonstrated that Lgr5 + stem cells per se are not essential for maintaining epithelial integrity 3 . (nature.com)
  • These more variable cell fates are thought to be due to the cells' interaction with the environment. (wikipedia.org)
  • Programming Blood Cell Fates. (lu.se)
  • Some organisms, such as C. elegans, have a predetermined pattern of cell progeny and the adult male will always consist of 1031 cells, this is because cell division in C. elegans is genetically determined and known as eutely. (wikipedia.org)
  • By 1976, Dr. Brenner and his associate, Dr. John Sulston, had identified part of the cell lineage in the developing nervous system of C. elegans. (wikipedia.org)
  • C. elegans contain 302 neurons and 959 somatic cells, where they begin with 1031, where 72 undergo apoptosis which is programmed cell death. (wikipedia.org)
  • Kim and Park, 2017 ) and make HMSCs attractive cells for regenerative therapies. (frontiersin.org)
  • CRISPR-based lineage recording methods are potentially scalable to whole-organism, with a single-cell resolution, and have produced high expectations in the developmental biology field. (irbbarcelona.org)
  • Nevertheless, while the basic principle of recorder-based lineage tree reconstruction is clear and intuitive, the accuracy of the lineages inferred by these methods has not been systematically evaluated. (irbbarcelona.org)
  • Our purpose is to quantify how accurate existing methods can be when reconstructing a cell lineage of tens of thousands of cells, trying to highlight some of the potential pitfalls when facing experimental constraints. (irbbarcelona.org)
  • Although MSC EVs show great promise for regenerative medicine applications, insights into their uptake mechanisms by different target cells and the ability to control MSC EV properties for defined function in vivo have remained elusive knowledge gaps. (frontiersin.org)
  • Naïve-like T-bet-experienced cells are polarized to the T H 1 lineage, predisposed to produce IFN-γ upon cell activation, and resist repolarization to other lineages in vitro and in vivo. (kcl.ac.uk)
  • The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. (nih.gov)
  • 3. Molecular Biology of the Cell Third Edition. (wormclassroom.org)
  • 4. Molecular Cell Biology - Fourth edition. (wormclassroom.org)
  • A long-standing aim in developmental biology has been to unravel an organism's full cell lineage, i.e., how cells relate to each other. (irbbarcelona.org)
  • State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China. (bvsalud.org)
  • This is based on the tracking of an organism's cellular ancestry due to the cell divisions and relocation as time progresses, this starts with the originator cells and finishing with a mature cell that can no longer divide. (wikipedia.org)
  • They made and studied bat iPS cells, finding insights into connections between hosts and viruses. (ipscell.com)
  • To improve the understanding of circulating influenza B lineages and influenza vaccine mismatches, we report the virus lineages circulating in northern Viet Nam over an eight-year period (2007-2014). (who.int)
  • As the two lineages have no cross-reactivity, the decision for vaccine lineage selection can be difficult in years when both influenza B lineages are circulating. (who.int)
  • Overall, the results presented in this study show a common mechanism of endocytosis for MSC EVs across different cell types and the feasibility to generate functionally enhanced EVs by modifications to parental MSCs. (frontiersin.org)
  • TDPCs exhibit bipotent progenitor behavior as they give rise to both squamous and neuroendocrine epidermal lineages, whereas the remainder of the α6 + Sca1 + CD200 - epidermis does not give rise to Merkel cells. (biologists.com)
  • However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. (nih.gov)
  • Borlongan, C.V., Glover, L.E., Tajiri, N., Kaneko, Y. and Freeman, T.B. (2011) The Great Migration of Bone Marrow-Derived Stem Cells towards the Ischemic Brain: Therapeutic Implications for Stroke and Other Neurological Disorders. (scirp.org)
  • That, they concluded, suggests the bone represents a previously unknown lineage of Homo sapiens with a morphology that is slightly different from AMH. (phys.org)
  • Polycythemia vera (PV) is a rare blood disease in which the bone marrow makes too many red blood cells. (cdc.gov)
  • This is accomplished by removal of blood through periodic phlebotomy and drug treatment to suppress red blood cell production by the bone marrow. (cdc.gov)
  • Patients with acute myelogenous leukemia (AML) present with symptoms resulting from bone marrow failure, symptoms resulting from organ infiltration with leukemic cells, or both. (medscape.com)
  • Patients with a high leukemic cell burden may present with bone pain caused by increased pressure in the bone marrow. (medscape.com)
  • The underlying pathophysiology in AML consists of a maturational arrest of bone marrow cells in the earliest stages of development. (medscape.com)
  • Gimble, J.M., Bunnell, B.A. and Guilak, F. (2012) Human Adipose-Derived Cells: An Update on the Transition to Clinical Translation. (scirp.org)
  • With our platform we develop and manufacture specialized, terminally-differentiated human cells from our pluripotent and progenitor cell starting materials. (lineagecell.com)
  • CD4+ T-cells and other lymphocyte subsets in persons infected with human immunodeficiency virus (HIV). (cdc.gov)
  • 1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunode- ficiency virus [HIV]. (cdc.gov)
  • Here we show the bioenergetics profile of human stem cells of embryonic origin differentiating along the hepatic lineage. (ku.dk)
  • Typically, these technologies start by engineering a single progenitor cell with artificial transcribed recording sites that accumulate stable insertions or deletions ("indels") as a result of repair of Cas9 double-stranded breaks. (biorxiv.org)
  • Several research groups have recently developed CRISPR/Cas9-based lineage recorders, which accumulate mutations as cells divide during development. (irbbarcelona.org)
  • These mutations are then used retrospectively to infer the lineage relationships between cells. (irbbarcelona.org)
  • They later received the 2002 Nobel prize for their work in genetic regulation of organ development and programmed cell death. (wikipedia.org)
  • These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage. (nih.gov)
  • Thus, our current understanding on how the expansion of particular B cell lineages by Env may be linked to the development of non-neutralizing antibodies is limited. (plos.org)
  • We report that the variable domains of recombinant HIV-1 Env immunogens activate a large number of B cell clones that give rise to many non-neutralizing antibodies, and that removing the variable domains from the immunogen reduces the number of activated B cell lineages and leads to the development of autologous neutralizing antibodies, a step towards bNAb-production. (plos.org)
  • Lineage's programs are based on the Company's proprietary cell-based therapy platform and associated development and manufacturing capabilities. (lineagecell.com)
  • In particular, it plays an important role in development of hematopoietic stem cells, early blood cells that have the potential to develop into all types of mature blood cells such as white blood cells, red blood cells, and platelets. (medlineplus.gov)
  • In our Phase 1/2a clinical trial, RG6501 (OpRegen) demonstrated the potential to slow, stop, or reverse the progression of GA in patients with GA. These results can be maintained beyond one year following a one-time, approximately 30-minute outpatient procedure," stated Brian M. Culley, Lineage CEO. (investorvillage.com)
  • Other organisms, such as humans, have variable lineages and somatic cell numbers. (wikipedia.org)
  • Other organisms had stereotyped patterns of cell division and produced sublineages which were the progeny of particular precursor cells. (wikipedia.org)
  • A pedigree of cells related through division that enable one to trace a cell's predecessors and progeny. (wormclassroom.org)
  • Due to new breakthroughs in tracking cells with greater accuracy, this aided the biological community since a variety of colors are now used in showing the original cells and able to track easily. (wikipedia.org)
  • Investigating lineage relationships alongside cell state and space would provide a fundamental understanding of these biological processes. (caltech.edu)