Cell types transfection. Medical search. Frequent questions

Avalanche® Transfection Reagent Series: Built on their expert knowledge & experience, powered by their vision & passion, EZ Biosystems™ has been dedicated to the research and development of next-generation transfection reagents by applying their expertise in combinatorial chemistry, molecular biology, and cell biology. (cambio.co.uk)
Check out the general purpose transfection reagents for plasmids, siRNAs (plasmid + siRNA co-transfections) and CRISPR/Cas9 as well as highly specific transfection reagents that have been carefully optimised for different primary cell types and different cell lines. (cambio.co.uk)
Why Cell Type-Specific Avalanche™ Transfection Reagents? (cambio.co.uk)
Other unknown properties that cause the responses of cells to transfection reagents vary significantly: some types of cells, such as suspension culture cells, primary cells, or stem cells are especially difficult to transfect with general-purpose tranfection reagents. (cambio.co.uk)
Cell type/cell line specific Avalanche ® transfection reagents - a series of transfection reagents that were designed and optimized specifically with ingredients, formulations, and protocols for each type of cell lines or primary cells, in order to ensure the highest levels of efficiency while keeping the toxicity levels minimal. (cambio.co.uk)
The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. (mdpi.com)
Find out the cell type/cell line specific Avalanche® transfection reagents by identifying the respective cell types or cell lines. (ezbiosystems.com)
Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. (thermofisher.com)
The reagents supplied in the Calcium Phosphate Transfection Kit (Product No. CAPHOS) are sterilized by 0.2 µM filter and aseptically filled. (sigmaaldrich.com)
To overcome major challenges and to meet your specific needs for transfection of various nucleic acids, we provide a comprehensive range of reagents for DNA transfection and DNA, mRNA, siRNA and miRNA transfection and cotransfection into a wide variety of cell lines, including sensitive primary cells. (qiagen.com)
Our optimized transfection reagents enable highly efficient cell transfection, giving you confidence in the results of your transient and stable transfection of a broad range of cell types, RNA interference studies, miRNA research and studies on gene expression and function. (qiagen.com)
Each of the cell type/cell line-specific transfection reagents in our Avalanche ® series was individually formulated. (cambio.co.uk)
Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment. (openpr.com)
Invitrogen Corporation has introduced a key product into its line of transfection reagents. (technologynetworks.com)
DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. (horizondiscovery.com)
siGENOME and ON-TARGET plus siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when demonstrated to have been used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100 �nM siRNA). (horizondiscovery.com)
TransIT®-293 Transfection Reagent - A High Efficiency, Low Toxicity, Plasmid DNA Transfection Reagent Optimized for 293 Cells ׀ Cell Line Specific DNA Transfection Reagents are formulated to maximize transfection efficiency while maintaining cellular health in many popular or hard-to-transfect cell types. (mobitec.com)
Indeed, research shows that transfecting some cell types with any plasmid may cause cell death [ 1 ], while some transfection reagents (e.g., lipids) used for plasmid transfection are themselves toxic to cells [ 2 ]. (idtdna.com)

With the launch of Avalanche® Transfection Reagent series, EZ Biosystems™ has been at the leading forefront of next-generation transfection reagent development and production. (cambio.co.uk)
Conclusion: It is impossible to create a universal transfection reagent that is able to work well on all cell types. (cambio.co.uk)
Trans IT™-Lenti Transfection Reagent is designed to enhance delivery of packaging and transfer vectors to adherent HEK 293T cell types and increase recombinant lentivirus production. (fishersci.com)
Fourth, the printed slide treated with transfection reagent is placed into a square dish with the printed side facing up. (wikipedia.org)
Effectene Reagent is used in conjunction with the enhancer and the DNA condensation buffer (Buffer EC) to achieve high transfection efficiency. (wikipedia.org)
The process of highly condensing DNA molecules and then coating them with Effectene Reagent is an effective way to transfer DNA into eukaryotic cells. (wikipedia.org)
The Effectene Transfection Reagent is intended for molecular biology applications. (qiagen.com)
Effectene Transfection Reagent is a nonliposomal lipid reagent for DNA transfection into a broad range of cell types. (qiagen.com)
Due to its low cytotoxicity, Effectene Transfection Reagent is highly suitable for transfection of primary cells and many sensitive cell lines. (qiagen.com)
Transfection using Effectene Transfection Reagent requires low amounts of DNA and minimal handling. (qiagen.com)
In addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. (qiagen.com)
Lipofectamine 3000 Transfection Reagent delivers a high efficiency for hard-to-transfect cell lines. (thermofisher.com)
When performing transfections in hard-to-transfect cell lines, Lipofectamine 3000 reagent can be an ideal choice. (thermofisher.com)
Lipofectamine 3000 reagent leverages our most advanced lipid nanoparticle technology to enable exceptional transfection performance and reproducible results. (thermofisher.com)
Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line. (thermofisher.com)
Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)
Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. (thermofisher.com)
The reagent allows for transfection to be performed both in presence or absence of serum. (thermofisher.com)
The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)
Avalanche ® - primary cell type-specific Transfection Reagent series? (cambio.co.uk)
TransIT-VirusGEN® Transfection Reagent by Mirus, USA, is designed to enhance delivery of packaging and transfer vectors to adherent and suspension HEK 293 cell types to increase recombinant adeno-associated virus (AAV) and lentivirus production. (openpr.com)
Lipofectamine™ LTX Reagent delivers DNA into the cell and demonstrates high levels of transfection efficiency and protein expression with 90 percent viability for a wide range of cells, including primary neuronal cells and other disease-relevant cell types. (technologynetworks.com)
Lipofectamine™ RNAi MAX is a RNAi-specific transfection reagent that is designed to offer high transfection efficiency and low toxicity profiles on a wide variety of cell types for siRNA gene knockdown experiments. (technologynetworks.com)
TransIT®-293 Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in HEK 293 and cell types of associated lineage. (mobitec.com)
Transfections with TransIT-293 Reagent do not require medium changes. (mobitec.com)

Luciferase expression was compared at 24 hours post-transfection using a standard luciferase assay. (bioscience.co.uk)
Images (32X) captured at 48 hours post-transfection. (bioscience.co.uk)
Images (63X) were captured at 24 hours post-transfection using a Nikon A1R confocal microscope. (bioscience.co.uk)
The amount of GAPDH or AHA1 mRNA was measured relative to 18s rRNA levels using qRT-PCR and then normalized to the mRNA levels of the non-targeting control, 48 hours post-transfection. (bioscience.co.uk)
A T7E1 mismatch detection assay was used to measure cleavage efficiency at 48 hours post-transfection. (bioscience.co.uk)
Analysis was performed 48 hours post transfection utilizing flow cytometry to determine percent transfection efficiency and the intensity of GFP expression. (thermofisher.com)
Typically, most of the cells that have not integrated the transfected plasmid will die while the cells that have undergone plasmid integration will survive by 9 days post-transfection. (mirusbio.com)
This method can be employed if a detectable marker is expressed on the cells post-transfection. (mirusbio.com)
PPIB or GAPDH down-regulation was determined using the Quantigene® branched DNA assay (Panomics) at 48 hours post-transfection. (horizondiscovery.com)

Finally, cultured cells are poured into the dish for plasmid uptake. (wikipedia.org)
TransI T-X2® from Mirus Bio is a low toxicity, dynamic transfection system for the superior transfection of plasmid DNA, siRNA, miRNA and CRISPR/Cas9 components into mammalian and primary cells. (bioscience.co.uk)
4-8 µl TransIT-X2™ used to transfect 2 µg plasmid DNA encoding EGFP into A549, CHO-K1, HepG2, LNCaP, MDCK, PC12, primary human mammary epithelial cells (HMEC) and normal human dermal fibroblasts (NHDF). (bioscience.co.uk)
TransIT-X2 was used to transfect plasmid Cy®5 labeled DNA encoding nuclear YFP and Cy®3 labeled siRNA into HeLa cells. (bioscience.co.uk)
The procedure stated below is designed for the transfection of CHO cells with 1 µg/µL pSV40-CAT plasmid (diluted in sterile molecular biology grade water). (sigmaaldrich.com)
Plasmid transfection in bovine cells: Optimization using a realtime monitoring of green fluorescent protein and effect on gene reporter assay. (oregonstate.edu)
However, this technology relies heavily on the insertion of foreign plasmid DNA into the nuclei of cells (i.e., transfection), which can be very challenging and highly variable among cell types. (oregonstate.edu)
Maximal transfection efficiency was obtained with 0.3μL/well of LipoLTX plus 25ng/well of plasmid DNA (ca. 29.5±1.9%) and 0.15μL/well of LipoLTX plus 25ng/well of plasmid DNA (ca. 4.0±0.4%) for MACT and MDBK cells, respectively. (oregonstate.edu)
Thus, factors such as TR type, TR amount, and DNA plasmid amount need to be optimized for a specific cell type before performing GRT assays. (oregonstate.edu)
The PureYield Plasmid Maxiprep System isolates transfection-quality plasmid DNA, providing a rapid method to purify up to 1mg of plasmid DNA from a 250ml bacterial culture. (promega.com)
The PureYield Plasmid Midiprep System isolates transfection-quality plasmid DNA, providing a rapid method to purify 100-200ug of plasmid DNA from a 50ml bacteria culture. (promega.com)
To enhance the osteogenic potential of stem cell sheet, we fabricated bone morphogenetic protein 2 ( BMP-2 ) gene-engineered cell sheet using a complex of polyethylenimine-alginate (PEI-al) nanocomposites plus human BMP-2 complementary(c)DNA plasmid, and studied its osteogenesis in vitro and in vivo. (dovepress.com)
Some cell types cannot tolerate plasmid transfection, so one important consideration is direct cytotoxicity due to plasmid transfection itself. (idtdna.com)
Importantly, it has also been shown that plasmid transfections, specifically in CRISPR experiments, can cause cytotoxicity in embryonic stem cells [ 3 ], which could be of concern to many researchers using this cell type. (idtdna.com)
Even with cells that can tolerate plasmid transfections, there are other problems associated with the use of plasmids for CRISPR editing, such as complications in the timing of CRISPR experiments. (idtdna.com)
Comparison of events after introducing CRISPR plasmid vs. ribonucleoprotein (RNP) into cells. (idtdna.com)
A ) When cells are transfected with a CRISPR plasmid, the plasmid is transported into the nucleus where transcription takes place. (idtdna.com)

RTF siRNA libraries are provided as multiple single-use plate sets - just rehydrate, and add cells. (horizondiscovery.com)

miRIDIAN microRNA Mimics designed to target the 3' UTR of either PPIB or GAPDH were transfected at 50 nM using DharmaFECT 1 into the indicated cell lines and assessed for their ability to decrease target mRNA levels. (horizondiscovery.com)
The miRIDIAN microRNA Mimic Endogenous Positive Control helps you monitor specific mimic effects on target protein levels in a validated endogenous assay, which is based upon the targeted activity of miR-122 on Aldolase A mRNA levels in cell lines. (horizondiscovery.com)
Gel shift experiments using nuclear extracts from TNF-alpha-treated cells and oligonucleotides containing the tTG nuclear factor (NF)-kappa B motif revealed increased binding, concordant with mRNA data. (nih.gov)
mRNA transfection is a novel solution for difficult-to-transfect cells such as neurons, primary cells and suspension cells. (bocsci.com)
Find mRNA transfection Kits with low toxicity at BOC Sciences! (bocsci.com)
mRNA, messenger ribonucleic acid, is a type of single-stranded ribonucleic acid that is transcribed from a strand of DNA as a template and carries genetic information that can guide protein synthesis. (bocsci.com)
Transfection of cells in culture with ASO targeting PTEN reduced PTEN mRNA and protein levels and increased insulin-stimulated Akt phosphorylation in α-mouse liver-12 (AML12) cells. (diabetesjournals.org)

It is suitable for both transient and stable transfection and can be used for many applications such as gene expression, viral production, shRNA expression, and promoter analysis. (mobitec.com)

In addition, miR‑205 overexpression reduced and miR‑205 depletion enhanced PC cell proliferation and migration in vitro. (spandidos-publications.com)
Notably, overexpression of RUNX2 partially reversed the inhibitory effect of miR‑205 on PC cell proliferation and migration in vitro. (spandidos-publications.com)
In addition, the results revealed that miR-205-induced downregulation of RUNX2 was associated with the inhibition of PC cell proliferation and migration. (spandidos-publications.com)
It is a complex and dynamic process to form a continuous construct via the cells' adhesion, proliferation, differentiation, and extracellular matrix (ECM) deposition on a scaffold. (dovepress.com)
Embryogenesis involves orchestrated processes of cell proliferation and differentiation. (biologists.com)
Embryogenesis depends on the timely proliferation of progenitor cells and their subsequent differentiation into multiple cell types. (biologists.com)
In many organs, cell proliferation and differentiation are antagonistically regulated by multiple basic helix-loop-helix (bHLH) activator and repressor genes. (biologists.com)
In this primer, we describe the key features of Hes factors and detail their roles in some representative processes of embryogenesis: namely, in the development of the nervous and digestive systems, two well-characterized processes, where Hes1 (and Hes3 and Hes5 in the nervous system) regulates cell proliferation and differentiation, and in the process of somite segmentation, where Hes7 functions as a biological clock. (biologists.com)
We previously reported that importin 13 (IPO13), a member of the importin-β family of nuclear import proteins, regulates nuclear import of the glucocorticoid receptor in airway epithelial cells, IPO13 serves as a potential marker for corneal epithelial progenitor cells, and IPO13 is associated with corneal cell proliferation. (molvis.org)
Here we investigated the role of IPO13 in the pathogenesis of pterygium and the underlying mechanism including interaction with other cell proliferation-related factors: keratin 17 (K17), a lesional protein and a member of the type I keratins, and c-Jun, a protein of the activator protein-1 complex. (molvis.org)
We examined the correlation between nuclear deformation and cell functions such as cell proliferation, transfection and extracellular matrix protein type I collagen production. (cdc.gov)
Further, we demonstrated that cell proliferation, transfection, and type I collagen production were strongly associated with the nuclear volume, indicating that the nucleus serves as a critical mechanosensor for cell regulation. (cdc.gov)
The findings showed that carbonate apatite did not affect the cell morphology of ADMPCs and that it promoted proliferation. (bvsalud.org)

Compatible with adherent and suspension cells, the animal-origin free Trans IT-X2® achieves both exceptional transient and stable transfections. (bioscience.co.uk)
This technique has been used to obtain both transient 1 and stable 2 transfections in a wide variety of cell types. (sigmaaldrich.com)
After stable transfection with E-cadher. (cellimagelibrary.org)
Cell line development is the process of creating stable cell lines for use in a variety of applications, including the creation of biologics (recombinant proteins and monoclonal antibodies), drug screening, and gene functional investigations. (alliedmarketresearch.com)
For instance, in April 2020, CEVEC Pharmaceuticals GmbH announced the launch of the ELEVECTA platform, which will produce stable cell lines for AAV (Adeno-associated viral) vector for gene therapy. (alliedmarketresearch.com)
These processes include transient transfection, and the creation of stable pools and clonal cell lines. (lonza.com)
Transient and stable pool processes are generally used for discovery through early-stage development to enable rapid production and screening of large numbers of protein candidates, while stable clonal cell lines are used for GMP clinical and commercial manufacturing. (lonza.com)

High toxicity: this is one of the major reasons that caused low efficiency in some sensitive cells. (cambio.co.uk)
Spectacular increase in transfection efficiency and dramatic decrease in cell toxicity. (thermofisher.com)
Examine the cells for visual toxicity daily. (mirusbio.com)
CONCLUSIONS Our results suggest that wild type huntingtin can significantly reduce the cellular toxicity of mutant HD exon 1 fragments in both neuronal and non-neuronal cell lines. (bmj.com)
This suggests that wild type huntingtin can be protective in different cell types and that it can act against the toxicity caused by a mutant huntingtin fragment as well as against a full length transgene. (bmj.com)
We were interested to test if wild type huntingtin protected against the toxicity of polyglutamine expansion mutations. (bmj.com)
Lipofectamine™ LTX is designed to provide effective delivery of DNA into a wide range of cell types and a significantly lower toxicity profile. (technologynetworks.com)

cell type-specific transfection protocols are available. (qiagen.com)

Recent work has demonstrated that clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems1-3 can serve as the basis of a simple and highly efficient method for performing genome editing in bacteria, yeast and human cells, as well as in vivo in whole organisms such as fruit flies, zebrafish and mice4-13. (cdc.gov)

Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. (ersjournals.com)
Reports from several laboratories suggest that a phenomenon termed epithelial-mesenchymal transformation, in which epithelial cells transform into fibroblasts capable of producing connective tissue matrix, is an important feature of tissue remodelling 10 , 11 . (ersjournals.com)
The changes that occur during adenoviral E1A transformation of primary guinea pig PLECs were also compared with those occurring during the transformation of the fibroblasts that frequently contaminate primary epithelial cultures, and evidence is presented that both cell types are transformed to an intermediate phenotype. (ersjournals.com)
We hypothesize that iPSC reprogrammed from ocular epithelial cells will be more efficient in differentiating into ocular lineages and exhibit higher functionality than non-ocular sources such as fibroblasts or blood. (stanford.edu)
RhoA has been implicated in the formation of stress fibers and cell adhesion in fibroblasts. (spandidos-publications.com)
We, herein, engineered nanotopographies of various shapes (gratings and pillars) and dimensions (feature size, spacing and height), and thoroughly investigated cell spreading, focal adhesion organization and nuclear deformation of human primary fibroblasts as the model cell grown on the nanotopographies. (cdc.gov)
In both healthy individuals and patients with Niemann-Pick disease types A and B, fibroblasts synthesize sphingomyelinase polypeptides with the same molecular mass of 110 kd, in the same amount. (medscape.com)

This feature ensures reproducibility of transfection-complex formation. (wikipedia.org)

The objective of this study was to investigate the optimal conditions to generate reliable GRT assay data on bovine immortalized cell lines, Madin Darby Bovine Kidney (MDBK) and bovine mammary epithelial alveolar (MACT) cells. (oregonstate.edu)
Using high, medium, and low transfection efficiency conditions determined in Experiment 1, we performed a GRT assay for peroxisome proliferator-activated response element (PPRE) luciferase in MACT and MDBK cells treated with 10nM or 100nM of synthetic Peroxisome Proliferator-activated Receptor β/σ (PPARβ/σ) agonist. (oregonstate.edu)
The GRT assay was unaffected by poor transfection in MACT cells although the high transfection hampered the possibility of detecting differences between 10 and 100nM of the PPARβ/δ agonist. (oregonstate.edu)
Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. (horizondiscovery.com)
Here, we use a human cell-based reporter assay to characterize off- target cleavage of Cas9-based RGENs. (cdc.gov)
Her research involves methods as tissue microarray (TMA) of colon cancer patients for immunohistochemistry (IHC) and Western blot, qPCR, transfection, luciferase assay, immunofluorescens on colon cancer cell lines. (lu.se)

As DNA is printed on a glass slide for the transfection process (the deliberate introduction of nucleic acids into cells) to occur before the addition of adherent cells, the order of addition of DNA and adherent cells is reverse that of conventional transfection. (wikipedia.org)

Transfection-EZ Biosystems: Why cell-type specific? (cambio.co.uk)

Ribonucleoprotein (RNP) complexes composed of PPIB (cyclophilin B) targeting two part gRNA (IDT) and Cas9 protein (PNA Bio) were delivered into HEK293T/17 and U2OS cells using TransIT-X2® Dynamic Delivery System or Lipofectamine® CRISPRMAX™ or Lipofectamine® RNAiMAX or Lipofectamine® 3000 in a 24-well format according to the manufacturers' protocol. (bioscience.co.uk)
Although Maraffini and colleagues7 recently performed a systematic investigation of Cas9 RGEN specificity in bacteria, the specificities of RGENs in human cells have not been extensively defined and, to our knowledge, bona fide off-target mutations induced by Cas9 have not been identified in any eukaryotic cell or organism. (cdc.gov)

Lipid cell signaling and critical metabolic enzymes: Antibodies and lipid recognition proteins, assays & enzymes, intracellular lipid delivery systems, isoprenoids, lipid-protein interaction tools, lysophospolipid products, MEP pathway intermediates, phosphoinositides & derivatives, phospholipase substrates and sphingolipid products. (openpr.com)
A hallmark of many of these diseases, including HD, spinobulbar muscular atrophy (SBMA), dentatorubral-pallidoluysian atrophy (DRPLA), and spinocerebellar ataxias (SCA) types 1, 2, 3, 6, and 7, is the development of intracellular protein aggregates (inclusions) in the vulnerable neurones. (bmj.com)
The Niemann-Pick group of diseases can be subclassified into two categories: (1) those with a primary deficiency in acid sphingomyelinase (ASM) activity (ie, types A and B) and (2) those with defective intracellular processing and transporting of low-density lipoprotein (LDL)-derived cholesterol (ie, type C). (medscape.com)
Transient transfection of intracellular Notch1 (Notch1-IC) into a pre-B cell line resulted in the down-regulation of EBF-regulated promoters and diminished the capacity of EBF to activate these promoters in an epithelial cell line. (lu.se)

Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)
Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
Furthermore, E1A DNA and protein persisted in epithelial cells in the lungs of these animals 9 . (ersjournals.com)
Currently we are collecting and growing epithelial cells from the ocular surface of donor eye tissues. (stanford.edu)
We intend to harness the inherent epigenetic memory of the ocular epithelial cells for superior and quicker protocol to regenerate cornea. (stanford.edu)
In this process, epithelial cells acquire fibroblast-like properties such as the functional loss of E-cadherin. (spandidos-publications.com)
Pterygium epithelial cells (PECs) were cultured in keratinocyte serum-free defined medium to examine the expression of IPO13 and K17. (molvis.org)

It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
Metaphase Kinetochore Dynamics: In this HeLa cell, which is expressing both a kinetochore marker (EGFP-CENP-B) and a centrosome marker (Venus-centrin), one can visualize the dynamic nature of kinetoch. (cellimagelibrary.org)
This HeLa cell is expressing two fluorescent proteins, including EGFP-CENP-B, a fluorescent protein that marks kinetochores (seen as paired dots oriented parallel to the x-axis and attached to opposit. (cellimagelibrary.org)
COP-I beta1 (green) staining at the Golgi (GM130, a cis-Golgi marker) (red), is redistributed out of the Golgi after EGF treatment of HeLa cells for 4 h. (cellimagelibrary.org)
GalNac-T1 staining (green) colocalizes with COP-I beta1 (red) staining at the Golgi (HPL) (gray) in unstimulated HeLa cells. (cellimagelibrary.org)
After EGF stimulation for 4 h, Helix Pomatia Lectin (HPL) (green) in HeLa cells expressing constitutively active mutant Arf1-GFP (Arf1(Q71L)-GFP) (gray) resists redistribution from Golgi (Giantin) (re. (cellimagelibrary.org)
HeLa cells transiently expressing GlcNAcT-1-HA-KDEL were fixed, immunolabeled for GlcNAcT-1-HA-KDEL (red) and endogenous alpha-mannosidase II (ManII, green), and examined by fluorescence microscopy. (cellimagelibrary.org)
Similar responses were seen in stably transfected HeLa cells. (nih.gov)

However, with the invention of different types of microarray printing systems, hundreds of transfection mixes (containing different DNA of interest) may be printed on the same slide for cell uptake of plasmids. (wikipedia.org)
Another timing issue caused by plasmids is that Cas enzyme and gRNA can remain active in the cells for prolonged periods. (idtdna.com)

Parallel cell seeding in a single chamber for 384 experiments, with no physical separation between experiments, increases screening data quality. (wikipedia.org)
While the experiments in the current paper were in progress, Leavitt et al 7 provided in vivo evidence suggesting that wild type huntingtin can protect against the gain of function mutation caused by the expanded polyglutamine tract in mutant huntingtin, using a YAC transgenic mouse model. (bmj.com)

Genes and Chromosomes Genes are segments of deoxyribonucleic acid (DNA) that contain the code for a specific protein that functions in one or more types of cells in the body or the code for functional ribonucleic. (msdmanuals.com)
In our present view, these areas are found for systems where several types of macromolecules (proteins, polysaccharides or polyelectrolytes) and/or amphiphiles interact to form a functional or destructive unit. (lu.se)

Total RNA from human cell lines or human fat was extracted with Trizol (Invitrogen) and purified via the RNeasy mini kit (Qiagen) according to the manufacturers' instructions. (nih.gov)

microRNA transfection using Lipofectamine RNiMAX (Invitrogen). (nih.gov)

The mammalian Hes basic helix-loop-helix repressor genes play central roles in these processes by maintaining progenitor cells in an undifferentiated state and by regulating binary cell fate decisions. (biologists.com)
In particular,the Hes bHLH repressor genes play an essential role in the development of many organs by maintaining progenitor cells and by regulating binary cell fate decisions. (biologists.com)
For example, in the developing nervous system of mouse embryos,progenitor cells proliferate and sequentially give rise to different types of cells by changing their differentiation competency. (biologists.com)
Without Hes genes such as Hes1 , however, progenitor cells prematurely differentiate into certain types of neurons only, and are depleted before they have proliferated sufficiently and generated all neuronal and glial cell types. (biologists.com)
We have developed an autologous transplantation method using adipose tissue-derived multi-lineage progenitor cells (ADMPCs) as a method of periodontal tissue regeneration that can be adapted to severe periodontal disease. (bvsalud.org)
In this study, we evaluated the safety and efficacy of adipose tissue-derived multi-lineage progenitor cells (ADMPC) autologous transplantation for periodontal tissue regeneration in humans. (bvsalud.org)

Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. (sigmaaldrich.com)
Nucleocytoplasmic transport is an essential phenomenon in eukaryotic cells controlled by the family of proteins called importins and exportins. (molvis.org)
Understanding the scope of RGEN-mediated off-target effects in human and other eukaryotic cells will be critically essential if these nucleases are to be used widely for research and therapeutic applications. (cdc.gov)

Culture cells in standard serum-containing or serum-free medium appropriate for the cell type. (sigmaaldrich.com)
An alternative in such cases is to use conditioned media and/ or 2X serum to increase cell attachment and survival. (mirusbio.com)
However, Rigamonti et al 6 recently showed that wild type huntingtin can protect CNS cells from a variety of apoptotic stimuli, including serum withdrawal, stimulation of death receptors, and pro-apoptotic Bcl-2 homologues. (bmj.com)
HEK 293 cells are commonly used for protein and virus production because they are well characterized, grow heartily in a variety of cell culture media (e.g. serum-free conditions) and are amenable to scale-up in biomanufacturing processes. (mobitec.com)

Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. (thermofisher.com)
An animation that takes the viewer inside a mammalian cell where one can see the nucleus and its characteristic envelope connected to the endoplasmic reticulum. (cellimagelibrary.org)
The sphingomyelin that accumulates in the lysosomes of Niemann-Pick disease-affected cells is thought to arise from the degradation of the cells and their organelles because it is a major component of all mammalian cell membranes. (medscape.com)

Cells were transfected according to each manufacturer's protocol. (bioscience.co.uk)
The Lipofectamine 3000 transfection protocol was developed to be easy to use, while still ensuring optimum performance and reliability in a wide panel of cell lines. (thermofisher.com)
This protocol can be optimized for use with a wide variety of cell types. (sigmaaldrich.com)

All four steps in the transfection process were optimized and our most advanced lipid nanoparticle technologies were combined. (thermofisher.com)

These technologies add significant capabilities to a transfection portfolio that is already widely adopted throughout the industry and was instrumental in establishing applications such as RNAi and stem cell research," said Claude Benchimol, Ph.D., Senior Vice President of Research and Development for Invitrogen. (technologynetworks.com)
Cornea is constantly regenerated from a small population of limbal epithelial stem cells via transiently amplifying cell (TAC) states. (stanford.edu)
Hematopoietic stem cell (HSC) replacement transplantation can ameliorate diseased hematologic system, thus inhibiting immune disorders including autoimmune diseases and immunodeficient disorders. (stanford.edu)
Recently, stem cell sheet has been an emerging strategy in bone tissue engineering. (dovepress.com)
PEI-al nanocomposites carrying BMP-2 gene could efficiently transfect bone marrow mesenchymal stem cells. (dovepress.com)
Our results suggested that PEI-al nanocomposites efficiently deliver the BMP-2 gene to bone marrow mesenchymal stem cells and that BMP-2 gene-engineered cell sheet is an effective way for promoting bone regeneration. (dovepress.com)
Recently, stem cell- and gene-based therapy has been applied in BTE and achieved some good clinical results. (dovepress.com)
Traditional BTE involves three-dimensional biodegradable scaffolds, stem cells, and osteoinductive growth factors. (dovepress.com)
Spinal Muscular Atrophies (SMAs) Spinal muscular atrophies are hereditary disorders in which nerve cells that originate in the spinal cord and brain stem degenerate, causing progressive muscle weakness and wasting. (msdmanuals.com)

Besides gelatin, atelocollagen and fibronectin are also successful transfection vectors for introducing foreign DNA into the cell nucleus. (wikipedia.org)

The Effectene-DNA complexes are mixed with the medium and directly added to the cells. (wikipedia.org)
These two cell types differed, however, in their NF-κB and AP-1 binding after lipopolysaccharide stimulation, possibly due to differences in the expression of the subunits that comprise these transcriptional complexes. (ersjournals.com)

Although scientists understand quite a bit of the transfection and gene expression processes as well as many signal pathways, there are many other aspects that remain unknown. (cambio.co.uk)
hundreds of genes may be expressed in cells on a single microarray for studying gene expression and regulation. (wikipedia.org)
The classic view of information processing in the cell by gene expression occurs through transcription followed by translation. (nature.com)
Another approach to gene therapy is to increase or decrease the activities of certain genes by using chemicals to modify chemical reactions in the cell that control gene expression. (msdmanuals.com)

Although nanotopography has been shown to be a potent modulator of cell behavior, it is unclear how the nanotopographical cue, through focal adhesions, affects the nucleus, eventually influencing cell phenotype and function. (cdc.gov)
Our study delineated the relationships between focal adhesions, nucleus and cell function and highlighted that the nanotopography could regulate cell phenotype and function by modulating nuclear deformation. (cdc.gov)
The normal DNA is inserted by a chemical reaction into the virus, which then infects (transfects) the person's cells, thereby transmitting the DNA into the nucleus of those cells. (msdmanuals.com)
Liposomes can be configured to contain DNA that can be absorbed by the person's cells, thereby delivering their DNA to the cell nucleus. (msdmanuals.com)

MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers research tools for molecular and cell biology. (openpr.com)

Here, we introduce PharmacoSTORM super-resolution imaging that combines the complementary advantages of these approaches and enables cell-type- and compartment-specific nanoscale molecular measurements. (nature.com)
Importantly, molecular alterations often swing into opposite directions in a cell- and subcellular compartment-specific manner. (nature.com)
We intended to clarify the phenotypic and molecular diversities of spinocerebellar ataxia type 2 (SCA2) in Japan. (nih.gov)
In an effort to advance the treatment strategies for the ocular surface diseases, I am focused on a regenerative medicine and single cell RNA sequencing based approaches to better comprehend the cellular biomarkers and molecular mechanisms involved in corneal tissue development and diseases. (stanford.edu)
cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)
basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)

miR-193b-3p could also be a predictive biomarker in chronic kidney disease undergoing radical nephrectomy for renal cell carcinoma [ 20 ]. (hindawi.com)

Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type. (horizondiscovery.com)

Respiratory or enteric viral isolates obtained from PIM specimens using poliovirus-permissive cell lines are also considered PIM. (cdc.gov)

Differentially expressed genes by miR-1285 transfection. (nih.gov)
In these processes, Hes genes function as effectors of Notch signaling, which coordinates cellular events via cell-cell interactions. (biologists.com)
Although gene therapy is defined as any treatment that changes gene function, it is often thought of as the insertion of normal genes into the cells of a person who lacks such normal genes because of a specific genetic disorder. (msdmanuals.com)

Transfection analyses were performed using a Schwann cell line, IMS32. (nih.gov)
Wild type huntingtin did not significantly modulate cell death caused by transfection of HD exon 1 fragments containing 21 CAG repeats in either cell line. (bmj.com)
The global Cell Line and Membrane Market size was valued at $4.5 billion in 2021, and is projected to reach $10.6 billion by 2031, growing at a CAGR of 8.8% from 2022 to 2031. (alliedmarketresearch.com)
Cell line is a general term that applies to a defined population of cells that can be maintained in culture for an extended period of time, retaining stability of certain phenotypes and functions. (alliedmarketresearch.com)
A cell line is a permanently established cell culture that will multiply indefinitely given appropriate fresh medium and space and is frequently used to investigate biological processes in place of primary cells. (alliedmarketresearch.com)
Transfection, selection, and single-cell cloning are all part of the cell line generation process. (alliedmarketresearch.com)
The overall impact of COVID-19 remains positive on Cell Line and Membrane market. (alliedmarketresearch.com)
Moreover, many government initiatives are conducted to reduce disease prevalence among animals which lead to growth of Cell Line and Membrane market. (alliedmarketresearch.com)
The key factors that drive the Cell Line and Membrane Market growth are rises in the prevalence of cancer and chronic diseases. (alliedmarketresearch.com)
Surprisingly, PITX2A activation of the ANF and PLOD1 promoters is repressed by co- transfection of Nkx2.5 in the C3H10T1/ 2 embryonic fibroblast cell line. (lu.se)

Brain circuits consist of more than hundred distinct neuronal and glial cell types 1 , which communicate via billions of synapses built up from highly compartmentalized nanoscale signaling platforms 2 . (nature.com)
METHODS We cotransfected neuronal (SK-N-SH, human neuroblastoma) and non-neuronal (COS-7, monkey kidney) cell lines with HD exon 1 (containing either 21 or 72 CAG repeats) construct DNA and either full length wild type huntingtin or pFLAG (control vector). (bmj.com)

Far better transfection efficiency than Lipofectamine® 2000 when we compared them head-to-head. (thermofisher.com)

One important insight came from the discovery that the increased motility and invasiveness of cancer cells is reminiscent of the epithelial-mesenchymal transition (EMT) that occurs during embryonic development ( 2 ). (spandidos-publications.com)

They are easy to culture and are commonly used in transfection and large-scale production of therapeutic proteins. (thermofisher.com)
The transfection study revealed that the 21-base-duplication mutant Ataxin-2 proteins aggregated in IMS32 and rendered cells susceptible to oxidative stress, similar to a CAG-expanded mutant. (nih.gov)
These proteins control the dynamics of the actin cytoskeleton and thus represent key regulatory molecules that are active during cell migration ( 12 ). (spandidos-publications.com)
Treatment of human Hep G2 cells with 1 ng/ml TNF-alpha increased [14C]putrescine cross-linking to cellular proteins. (nih.gov)
It has been suggested that this process relies on Notch's ability to inhibit E proteins, which are crucial for early B-cell development. (lu.se)

Using cloning rings and/or trypsin discs is a viable option for adherent cells in this scenario. (mirusbio.com)

The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. (qiagen.com)
Keep replacing media containing selection antibiotic twice a week until the cells in the T75 flask reach high confluence. (mirusbio.com)
The perturbances in cellular redox homeostasis leads to high free radical burden thereby causing oxidative stress in the cells. (stanford.edu)

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. (cambio.co.uk)
All of NCI60 cancer cell lines). (cambio.co.uk)
RESULTS Full length wild type huntingtin significantly reduced cell death resulting from the mutant HD exon 1 fragments containing 72 CAG repeats in both cell lines. (bmj.com)
To address this issue, the expression levels of Snail, RhoA and fibronectin, as well as MMP‑2, were reduced in the breast tumor cell lines MDA‑MB‑231 and MDA‑MB‑435S, and their biological responses were studied in vitro and in vivo. (spandidos-publications.com)
Cell lines are usually clonal, meaning that the entire population originated from a single common ancestor cell. (alliedmarketresearch.com)
Distinct types of cells are used to create different cell lines. (alliedmarketresearch.com)
Moreover, the market is expected to experience growth during the forecast period owing to the rise in development and demand for monoclonal antibodies around the globe, the rise in vaccine production, and technological innovations in cell lines and the membrane. (alliedmarketresearch.com)
Also, PIM cultured in some common cell lines to isolate other viruses of interest may unintentionally amplify poliovirus. (cdc.gov)
Deliver 2 clonally expanded mycoplasma free iPSC lines (15 x 300k cells). (lu.se)

Using these variants, we map and validate gene-disease associations in two independent biobanks containing exome sequencing from 10,900 and 32,268 individuals, respectively, and elucidate their impact on protein expression in human cells. (nature.com)

Anatomy42

Organisms17

Diseases6

Chemicals and Drugs81

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes72

Disciplines and Occupations2

Technology, Industry, Agriculture5

Information Science3

Reagents18

Reagent27

Post-transfection9

Plasmid17

SiRNA1

MRNA7

Transient and stable transfection1

Proliferation13

Stable7

Toxicity7

Specific transfection1

CRISPR1

Fibroblasts8

Reproducibility1

Assay6

Nucleic1

Biosystems1

Cas92

Intracellular4

Epithelial cells7

HeLa8

Plasmids2

Experiments2

Functional2

QIAGEN1

MicroRNA1

Progenitor Cells6

Eukaryotic3

Serum4

Mammalian cell3

Protocol3

Lipid1

Stem9

Vectors1

Complexes2

Gene expression4

Nucleus4

Biology1

Molecular6

Renal cell carc1

DharmaFECT1

Isolates1

Genes3

Line10

Neuronal2

Lipofectamine1

Embryonic1

Proteins5

Adherent cells1

High3

Lines9

Protein expression1