• tumor cells
  • Dr. Neal Rosen and colleagues from Memorial Sloan-Kettering Cancer Center in New York examined interplay between cell proliferation signals in tumor cells with PTEN mutations. (innovations-report.com)
  • Tumor cells with mutated PTEN did not respond to treatment with EGFR inhibitors, but when PTEN activity was restored, tumor cells were sensitized to treatment with EGFR inhibitors. (innovations-report.com)
  • Consistent with its widespread expression on normal cells, CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary. (wikipedia.org)
  • Inhibition of AC activity sensitizes tumor cells to the effects of antineoplastic agents and radiation. (wikipedia.org)
  • protein
  • The protein, called LHPP, prevents the uncontrolled proliferation of cancer cells in the liver. (innovations-report.com)
  • or you can do the vitality assay on cells with different numbers than normalize your assay with total protein or nucleic acid contents. (protocol-online.org)
  • Because I used MTT for cell viability, I can not use the exact same well for protein assay. (protocol-online.org)
  • In summary, we uncovered a signalling pathway operating specifically in T-ALL cells in which ORP4L mediates G protein-coupled ligand-induced PLCβ3 activation, resulting in an increase of mitochondrial respiration for cell survival. (nature.com)
  • This study adds to our previously published work because it implicates the TNF family member protein called BAFF in the 'revved up' B-cell signaling we found in our patients. (eurekalert.org)
  • I also investigated the repression of the transcription factor c-Jun, by itself and by Krox-20, in immature Schwann cells and found that this occurs mainly at the protein, rather than the transcriptional level. (bl.uk)
  • July 22, 2009 - (BRONX, NY) - A cellular protein that may prevent nerve cells from dying also helps to improve insulin action and lower blood glucose levels, according to a study by researchers at Albert Einstein College of Medicine of Yeshiva University in collaboration with scientists at University of California, Los Angeles (UCLA). (yu.edu)
  • We further identify the 3-phosphoinositide-dependent protein kinase 1 (PDK1), a well-known kinase involved in the PI3K-Akt signaling pathway, as a direct Meis3 target, which mediates its role in β-cell survival. (biomedsearch.com)
  • The low-affinity nerve growth factor receptor (nerve growth factor receptor (TNFR superfamily, member 16), also called the LNGFR or p75 neurotrophin receptor) is one of the two receptor types for the neurotrophins, a family of protein growth factors that stimulate neuronal cells to survive and differentiate. (wikipedia.org)
  • Survival and PCD mechanisms are mediated through adaptor protein binding to the death domain of the p75NTR cytoplasmic tail. (wikipedia.org)
  • In 2008, Carol A Mercer created a BHMT fusion protein (GST-BHMT), which showed starvation-induced site-specific fragmentation in cell lines. (wikipedia.org)
  • This protein domain is highly important to cell survival. (wikipedia.org)
  • The β-cells promote their protein transcription in response to nutrients. (wikipedia.org)
  • Studies in Xenopus oocytes suggest that this protein alone and in combination with pannexin 2 may form cell type-specific gap junctions with distinct properties. (wikipedia.org)
  • endothelial cells
  • 6-10 Evidence suggests that EPCs contribute as much as 25% of endothelial cells (ECs) in newly formed blood vessels, 11 and transplantation of EPCs into patients has been demonstrated to induce blood flow recovery in ischemic limbs 12 and increase myocardial viability after infarction. (ahajournals.org)
  • inhibits
  • This process inhibits the ATP-sensitive potassium ion channels of the cell causing the Potassium ion channel to close and not function anymore. (wikipedia.org)
  • apoptotic
  • At the end of lactation, the loss of the suckling triggers a STAT3-dependent apoptotic program to remove the differentiated alveolar cells. (nih.gov)
  • intracellular
  • Ion leakage across cell membranes occurs as a result of both intracellular and extracellular ions drifting towards their thermodynamic equilibrium. (biologists.org)
  • The rise in free cytosolic intracellular Ca 2+ concentration results in the activation of Ca 2+ -dependent phospholipases and proteases that further hasten the rate of membrane depolarisation, leading to uncontrolled cellular swelling and, ultimately, to cell necrosis ( Hochachka, 1986 ) ( Fig.1 ). (biologists.org)
  • ultimately
  • Chapel Hill, NC - Leukemia and lymphoma patients who receive life-saving stem cell or bone marrow transplants often experience chronic side effects that significantly decrease quality of life, can last a lifetime, and ultimately affect their long-term survival. (eurekalert.org)
  • Ultimately, differential targeting of this process may promote survival of postmitotic tissue or enhance tumor-specific killing. (sciencemag.org)
  • stimulation
  • This raises the issue of the role that PLCβs versus PLCγ1 play in Ca 2+ homeostasis of T-ALL cells and how PLCβs are activated upon anti-CD3 stimulation of these cells. (nature.com)
  • I found that STAT3 is activated by, and supports survival following stimulation by autocrine factors secreted by Schwann cells both in vitro and following nerve injury in vivo. (bl.uk)
  • Metabolic
  • Jessica Allen, PhD, the paper's first author, says "We found that B cells from patients with active chronic GVHD were in a heightened metabolic state and resist programmed cell death. (eurekalert.org)
  • The single most protective and unifying feature of cold-tolerance in hibernating and neonatal mammals is the same one that ensures their enhanced survival in O 2 -limiting conditions: a regulated metabolic depression. (biologists.org)
  • In contrast, an early mismatch between ATP supply and demand in 'anoxia- and cold-sensitive cells' leads to a forced hypometabolism which is, in effect, early metabolic failure. (biologists.org)
  • Instead, these data define a central role for Lkb1 in restricting HSC entry into cell cycle and in broadly maintaining energy homeostasis in haematopoietic cells through a novel metabolic checkpoint. (pubmedcentralcanada.ca)
  • The metabolic control systems in HSCs are poorly understood although these cells have been shown to be highly sensitive to energetic and oxidative stress and must be able to shift between quiescence and highly proliferative states to maintain the haematopoietic system under varying physiological conditions 1 - 5 . (pubmedcentralcanada.ca)
  • Given the importance ofHSCdynamics to the haematopoietic system and the sensitivity of haematopoietic cells to metabolic stress, we sought to investigate the function of Lkb1 in haematopoiesis. (pubmedcentralcanada.ca)
  • neuron
  • NGF is released from the target cells, binds to and activates its high-affinity receptor tropomyosin receptor kinase A (TrkA), and is internalized into the responsive neuron. (wikipedia.org)
  • receptors
  • However, NGF binds at least two receptors on the surface of cells that are capable of responding to this growth factor, TrkA (pronounced "Track A") and the LNGFR. (wikipedia.org)
  • Cells which express both the LNGFR and the Trk receptors might therefore have a greater activity - since they have a higher "microconcentration" of the neurotrophin. (wikipedia.org)
  • In the beta-cells of the pancreas, glucose enters through the GLUT 2 receptors (process described below). (wikipedia.org)
  • inhibitor
  • And what is the inhibitor for EDTA after cells are detached? (protocol-online.org)
  • 66, 6800-6806 Sun, Y., Hegamyer, G. and Colburn, N. H. (1994) Molecular cloning of five messenger RNAs differentially expressed in preneoplastic or neoplastic JB6 mouse epidermal cells: one is homologous to human tissue inhibitor of metalloproteinases-3. (wikipedia.org)
  • transcription
  • AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. (wikipedia.org)
  • TrkA
  • The NGF/TrkA complex is subsequently trafficked back to the cell body. (wikipedia.org)
  • TrkA is a receptor tyrosine kinase (meaning it mediates its actions by causing the addition of phosphate molecules on certain tyrosines in the cell, activating cellular signaling). (wikipedia.org)
  • Study results indicate that superior cervical ganglia neurons that express both p75NTR and TrkA die when treated with proNGF, while NGF treatment of these same neurons results in survival and axonal growth. (wikipedia.org)
  • induces
  • In the process of inflammation, NGF is released in high concentrations by mast cells, and induces axonal outgrowth in nearby nociceptive neurons. (wikipedia.org)
  • stem cells
  • For patients who received bone marrow or stem cells, it is estimated that 40-70 percent may experience chronic GVHD. (eurekalert.org)
  • Haematopoietic stem cells (HSCs) can convert between growth states that have marked differences in bioenergetic needs. (pubmedcentralcanada.ca)
  • An image of a muscle implanted shows pre-existing muscle fibers (green cells only), along with fibers created by transplanted stem cells (green fiber with red membrane). (medicalxpress.com)
  • Medical Xpress) -- Controlling the amount of oxygen that stem cells are exposed to can significantly increase the effectiveness of a procedure meant to combat an often fatal form of muscular dystrophy, according to Purdue University research. (medicalxpress.com)
  • A genetic mutation in patients with Duchenne muscular dystrophy causes the constant breakdown of muscles and gradual depletion of stem cells that are responsible for repairing the damage and progressive muscle wasting. (medicalxpress.com)
  • Stem cell therapy - implanting healthy stem cells to combat tissue wasting - has shown promise against muscular dystrophy and other neurodegenerative diseases , but few of the implanted stem cells survive the procedure. (medicalxpress.com)
  • Shihuan Kuang, a Purdue assistant professor of animal sciences, and Weiyi Liu, a postdoctoral research associate, showed that survival of implanted muscle stem cells could be increased by as much as fivefold in a mouse model if the cells are cultured under oxygen levels similar to those found in human muscles . (medicalxpress.com)
  • Stem cells survive in a microenvironment in the body that has a low oxygen level," Kuang said. (medicalxpress.com)
  • When we did that, we saw that more stem cells survived the transplant. (medicalxpress.com)
  • Liu thinks that's because the stem cells grown in higher oxygen levels acclimate to their surroundings. (medicalxpress.com)
  • In the mouse model, Kuang and Liu saw more stem cells survive the transplants, and those stem cells retained their ability to duplicate themselves. (medicalxpress.com)
  • If these stem cells self-renew, they should never be used up and should continue to repair damaged muscle. (medicalxpress.com)
  • Satellite cells are muscle-resident stem cells that maintain the homeostasis and mediate the regeneration of skeletal muscles. (medicalxpress.com)
  • LNGFR has been implicated as a marker for cancer stem cells in melanoma and other cancers. (wikipedia.org)
  • survive
  • It prevents the cell communicating the stress that it is under, which is crucial if the cell is to survive. (wikipedia.org)
  • Truncating mutations in this gene have been shown to promote breast cancer metastasis to the lungs by allowing cancer cells to survive mechanical stretch in the microcirculation. (wikipedia.org)
  • homeostasis
  • Here we show that the Lkb1 tumour suppressor is critical for the maintenance of energy homeostasis in haematopoietic cells. (pubmedcentralcanada.ca)
  • Lkb1 can also regulate energy homeostasis and cell structure through its capacity to phosphorylate and activate 12 other AMPK-related kinases 11 . (pubmedcentralcanada.ca)
  • The insulin transduction pathway is a biochemical pathway by which insulin increases the uptake of glucose into fat and muscle cells and reduces the synthesis of glucose in the liver and hence is involved in maintaining glucose homeostasis. (wikipedia.org)
  • neural crest
  • The generation of mature Schwann cells from the neural crest occurs by a transition through two intermediate cell types, namely the Schwann cell precursor and the immature Schwann cell. (bl.uk)
  • Gene knockdown of CD271 has also been shown to abolish neural crest stem cell properties of melanoma cells. (wikipedia.org)
  • STAT3
  • I examined the role of STAT3 in Schwann cells both in vitro and in vivo using mice with a conditional mutation of STAT3 specifically in Schwann cells. (bl.uk)
  • immune
  • In chronic Graft vs. Host Disease (GVHD), the differences between the donor bone marrow cells and the recipient's body often cause these immune cells to recognize the recipient's body tissues as foreign and the newly transplanted cells attack the transplant recipient's body. (eurekalert.org)
  • It also was found to reduce the amount of aging in yeast, flies, worms, and human immune cells by inducing autophagy. (wikipedia.org)
  • This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. (wikipedia.org)
  • membrane
  • In cases of severe O 2 limitation, most excitable cells of mammals cannot continue to meet the energy demands of active ion-transporting systems, leading to rapid exhaustion of fermentable substrate, catastrophic membrane failure and cell death. (biologists.org)
  • The closure of the ATP-sensitive potassium channels causes depolarization of the cell membrane causing the cell membrane to stretch which causes the voltage-gated calcium channel on the membrane to open causing an influx of Ca2+ ions. (wikipedia.org)
  • vivo
  • ORP4L knockdown results in suboptimal bioenergetics, cell death and abrogation of T-ALL engraftment in vivo . (nature.com)
  • binds
  • The secondary signal is CD40L (CD154) on the T cell which binds CD40 on the macrophage cell surface. (wikipedia.org)
  • If an activated T cell recognizes the peptide presented by the B cell, the CD40L on the T cell binds to the B cell's CD40 receptor, causing B cell activation. (wikipedia.org)
  • When insulin binds to the insulin receptor, it leads to a cascade of cellular processes that promote the usage or, in some cases, the storage of glucose in the cell. (wikipedia.org)
  • growth
  • Cells were cultured in endothelial cell basal medium-2 in the absence and presence of CRP (5 to 20 μg/mL), rosiglitazone (1 μmol/L), and/or vascular endothelial growth factor. (ahajournals.org)
  • The Lkb1 tumour suppressor encodes an evolutionarily conserved serine/threonine kinase that links metabolism and cell growth 6 . (pubmedcentralcanada.ca)
  • Two distinct subpopulations of Olfactory ensheathing glia (being researched for nerve repair) have been identified with high or low cell surface expression of low-affinity nerve growth factor receptor (p75). (wikipedia.org)
  • This is useful for control of cellular signaling because the di-phosphorylated phosphoinositide PIP2 is only phosphorylated by the family of enzymes, PI 3-kinases (phosphoinositide 3-kinase or PI3-K), and only upon receipt of chemical messengers which tell the cell to begin the growth process. (wikipedia.org)
  • Expression
  • Lkb1 mRNA expression is readily detectable in haematopoietic cells in the bone marrow, with highest levels in HSCs 12 and in multipotent and lineage-restricted progenitors compared to committed Lin + cells ( Supplementary Fig. 1a ). (pubmedcentralcanada.ca)
  • Melanoma cells transplanted into an immunodeficient mouse model were shown to require expression of CD271 in order to grow a melanoma. (wikipedia.org)
  • Spermidine has also been found to "upregulate expression of the epithelial stem cell-associated keratins K15 and K19, and dose-dependently modulated K15 promoter activity in situ and the colony forming efficiency, proliferation and K15 expression of isolated human K15-GFP+ cells in vitro. (wikipedia.org)
  • concentrations
  • Whereas the time course of the latter is metered in minutes to hours, the ability to depress ATP turnover in a regulated fashion effectively delays the onset of cell death by stabilising concentrations of adenylates at new hypometabolic steady states for periods of hours or days ( Fig.1 ). (biologists.org)
  • donor
  • The technique involves injection of compatible donor red blood cells of a different blood group into a recipient, followed by blood testing periodically afterwards. (wikipedia.org)
  • cytoplasm
  • Humanin is found in mitochondria - structures that populate the cytoplasm of cells and provide them with energy. (yu.edu)
  • Lysosomes could not be cell organelles, but part of cytoplasm such as mitochondria, and that hydrolytic enzymes were produced by microbodies. (wikipedia.org)
  • pathway
  • Two aspects of the transduction pathway process are explained below: insulin secretion and insulin action on the cell. (wikipedia.org)
  • insulin
  • In the islets of Langerhans, there are beta-cells, which are responsible for production and storage of insulin. (wikipedia.org)
  • vitro
  • In this study I investigated the effects of BMP in the Schwann cell lineage in vitro. (bl.uk)
  • hypoxic
  • Microenvironmental oxygen (O2) regulates stem cell activity, and a hypoxic niche with low oxygen levels has been reported in multiple stem cell types. (medicalxpress.com)
  • We demonstrate here that hypoxic culture conditions favor the quiescence of satellite cell-derived primary myoblasts by upregulating Pax7, a key regulator of satellite cell self-renewal, and downregulating MyoD and myogenin. (medicalxpress.com)
  • bacteria
  • A team of biologists at the Stanford University School of Medicine employed a new method to estimate the minimum genetic information required for the survival of bacteria called Caulobacter crescentus , which are commonly used in labs. (icr.org)
  • populations
  • ORP4 knockout mice exhibit teratozoospermia due to death of developing spermatozoa, indicating that ORP4 is essential for the survival of specific cell populations 19 . (nature.com)
  • tissue
  • Depending on the tissue type, the glucose enters the cell through facilitated or passive diffusion. (wikipedia.org)