• Flow
  • In this dissertation a non-adherent immortal cell line was studied under static media flow conditions inside a CO[subscript]2 incubator and under dynamic media flow conditions in a novel portable cell culture chamber. (k-state.edu)
  • In addition to cell trapping, a novel electrokinetic injection method was developed for injecting analytes from a hydrodynamic flow into a separation channel that was followed by an electrokinetic separation. (k-state.edu)
  • As the hydrodynamic flow could introduce some excess band broadening in the separation, the actual band broadening of an analyte was measured for different channel depths and hydrodynamic fluid flow rates. (k-state.edu)
  • method
  • and C) using this novel injection method to lyse single non-adherent cells by applying a high electric field across the cell at a microfluidic channel intersection. (k-state.edu)
  • Finally, using this novel injection method, single cell lysis was performed by applying a high voltage at the microfluidic channel intersection. (k-state.edu)
  • different
  • To attempt to successfully trap cells, three different types of cellular traps were designed, fabricated and tested in polydimethylsiloxane (PDMS)-based microfluidic devices. (k-state.edu)
  • analysis
  • There are several potential advantages to the use of microfluidic devices for the analysis of single cells: First, cells can be handled with care and precision while being transported in the microfluidic channels. (k-state.edu)
  • Third, cell culturing and analysis in microfluidic devices uses only extremely small volumes of culturing media and analysis buffer. (k-state.edu)