• protein
  • Fucosyl-GM1 expression and amyloid-beta protein accumulation in PC12 cells. (biomedsearch.com)
  • We found that overexpression of tail interacting protein of 47 kDa (TIP47), but not its truncated form (t-TIP47) protected NIH3T3 cells from hydrogen-peroxide-induced cell death, prevented the hydrogen-peroxide-induced mitochondrial depolarization determined by 5,50,6,60-tetrachloro-1,10,3,30-tetraethyl-benzimidazolylcarbocyanine iodide (JC1), while suppression of TIP47 in HeLa cells facilitated oxidative-stress-induced cell death. (biomedsearch.com)
  • PGA13 encodes a GPI protein in the human pathogen Candida albicans, which is highly up-regulated during cell wall regeneration in protoplasts. (biomedsearch.com)
  • We report that protein adsorption, cell attachment, and cell proliferation were enhanced on spherulites-roughened polymer surfaces. (biomedsearch.com)
  • Cytoprotection following endoplasmic reticulum stress protein induction in continuous cell lines. (biomedsearch.com)
  • GPR40, which has recently been identified as a G-protein-coupled cell-surface receptor for long-chain fatty acids, was assessed in a human breast cancer cell line (MCF-7). (biomedsearch.com)
  • Two-color labeling of temporally defined protein populations in mammalian cells. (biomedsearch.com)
  • Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. (wikipedia.org)
  • The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. (wikipedia.org)
  • During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. (wikipedia.org)
  • Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting (quiescent) cells (G0). (wikipedia.org)
  • Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. (wikipedia.org)
  • The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the Hodgkin lymphoma cell line L428. (wikipedia.org)
  • Protein Ki-67 in human MCF-7 cells. (wikipedia.org)
  • Ki-67 protein (red), tubulin (green) and DNA (blue) in HeLa cells. (wikipedia.org)
  • Cdh1 is one of the co- activator proteins of APC/c and therefore contributes to the regulation of protein degradation, by providing substrate specificity to the E3-ligase in a cell cycle regulated manner. (wikipedia.org)
  • The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. (wikipedia.org)
  • Low density lipoprotein receptor-related protein 1 (LRP1), also known as alpha-2-macroglobulin receptor (A2MR), apolipoprotein E receptor (APOER) or cluster of differentiation 91 (CD91), is a protein forming a receptor found in the plasma membrane of cells involved in receptor-mediated endocytosis. (wikipedia.org)
  • tissues
  • Multicellular organisms arise from the generation of different cell types and the organization of cells into tissues and organs. (biomedsearch.com)
  • Here, we show that cellular half-life in tissues can be histologically measured under steady state conditions in vivo by analyzing the loss of 5-bromo-2'-deoxyuridine (BrdU)-labeled cells over time after withdrawal of long-term BrdU labeling. (biomedsearch.com)
  • LRP1 is a member of the LDLR family and ubiquitously expressed in multiple tissues, though it is most abundant in vascular smooth muscle cells (SMCs), hepatocytes, and neurons. (wikipedia.org)
  • abnormal
  • When those genes suffer some kind of mutation or translocation, they can change their conformation and cause a catalytic activity in cell reproduction that is normally inactivated, which causes abnormal cell proliferation. (wikipedia.org)
  • Tumor endothelial cells (TECs) have been documented to demonstrate abnormal morphologic characteristics such as ragged margins and irregular cytoplasmic projections. (wikipedia.org)
  • Loss of pericyte growth factor (PDGFB) and its receptor on endothelial cells are molecular-level changes that can account for this abnormal loss in pericyte support. (wikipedia.org)
  • Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z. Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. (wikipedia.org)
  • A549
  • The results showed that betulin significantly inhibited cell viability in cervix carcinoma HeLa cells, hepatoma HepG2 cells, lung adenocarcinoma A549 cells, and breast cancer MCF-7 cells with IC(50) values ranging from 10 to 15 microg/mL. (biomedsearch.com)
  • tumor cells
  • The fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. (wikipedia.org)
  • Research supports that compression of tumor vessels by surrounding tumor cells results in mechanical tension and changes in blood flow. (wikipedia.org)
  • Glutaminolysis takes place in all proliferating cells, such as lymphocytes, thymocytes, colonocytes, adipocytes and especially in tumor cells. (wikipedia.org)
  • In tumor cells the citric acid cycle is truncated due to an inhibition of the enzyme aconitase (EC 4.2.1.3) by high concentrations of reactive oxygen species (ROS) Aconitase catalyzes the conversion of citrate to isocitrate. (wikipedia.org)
  • On the other hand, tumor cells over express phosphate dependent glutaminase and NAD(P)-dependent malate decarboxylase, which in combination with the remaining reaction steps of the citric acid cycle from α-ketoglutarate to citrate impart the possibility of a new energy producing pathway, the degradation of the amino acid glutamine to glutamate, aspartate, pyruvate CO2, lactate and citrate. (wikipedia.org)
  • Besides glycolysis in tumor cells glutaminolysis is another main pillar for energy production. (wikipedia.org)
  • Due to low glutamate dehydrogenase and glutamate pyruvate transaminase activities, in tumor cells the conversion of glutamate to alpha-ketoglutarate mainly takes place via glutamate oxaloacetate transaminase. (wikipedia.org)
  • Glutamine is the most abundant amino acid in the plasma and an additional energy source in tumor cells especially when glycolytic energy production is low due to a high amount of the dimeric form of M2-PK. (wikipedia.org)
  • The release of both metabolites may protect tumor cells from immune attacks. (wikipedia.org)
  • mammalian
  • Although the mechanisms and proteins of mitochondrial fusion are well known in yeast and mammalian cells, they remain poorly understood in plant cells. (biomedsearch.com)
  • Up to the eight-cell embryo stage, the blastomeres are morphologically identical and distributed symmetrically in the mammalian embryo. (biomedsearch.com)
  • Vector injection into the perivitelline space has emerged as the standard delivery method to transduce lentivirus to mammalian oocytes or one-cell embryos, but its application is limited by the need for high titers of lentivirus. (biomedsearch.com)
  • populations
  • Tissue remodeling in development and disease involves the coordinated invasion of neighboring territories and/or the replacement of entire cell populations. (biomedsearch.com)
  • antigen
  • Most authors currently quantify the frequency of apoptotic cells in a given phenotypically defined population after calculating the apoptotic index (AI), i.e., the percentage of apoptotic cells displaying a specific linage antigen (LAg) within a population of cells that remain unfragmented and retain the expression of the LAg. (biomedsearch.com)
  • Ki-67 and MIB-1 monoclonal antibodies are directed against different epitopes of the same proliferation-related antigen. (wikipedia.org)
  • stimulation
  • Since exogenous testosterone can suppress LH levels, it could be that androgen therapy could revert the LH-induced growth stimulation of Leydig cell compartment. (clinicaltrials.gov)
  • BACKGROUND AND OBJECTIVE: Periodontal ligament (PDL) cells show traits that are typical of osteoblasts, such as osteoblastic marker gene expression and the ability to respond to parathyroid hormone (PTH) stimulation in an osteoblast-like manner with respect to differentiation and local factor production. (biomedsearch.com)
  • nuclei
  • Cell type-specific affinity purification of nuclei for chromatin profiling in whole animals. (biomedsearch.com)
  • Somatic nuclei can be reprogrammed into a pluripotent state by nuclear transfer, cell fusion and expression of transcription factors. (biomedsearch.com)
  • In various polarized cells, positions of nuclei are often off-center. (biomedsearch.com)
  • Dividing cells show strong Ki-67 staining in cell nuclei while all cells contain large amounts of tubulin, the major component of microtubules. (wikipedia.org)
  • induces
  • The 1-methyl-4-phenyl-pyridinium ion (MPP(+)), an active metabolite of the neurotoxin, N-methyl-4-phenyl-1,2,3,6 tetrahydropyridine (MPTP), induces death in rat pheochromocytoma (PC12) cells, suggesting a cell model of Parkinson's disease (PD). (biomedsearch.com)
  • Prolonged p21 up-regulation induces a senescent phenotype in normal and cancer cells, accompanied by an increase in intracellular reactive oxygen species (ROS). (biomedsearch.com)
  • A Jun amino-terminal kinase (JNK) cascade in the DME cells induces the expression of Decapentaplegic (Dpp). (biomedsearch.com)
  • apoptotic cells
  • TUNEL is a method for detecting apoptotic DNA fragmentation, widely used to identify and quantify apoptotic cells, or to detect excessive DNA breakage in individual cells. (wikipedia.org)
  • progenitor cells
  • We have analyzed the localization of label-retaining cells (LRCs), which are putative progenitor cells, by using the BrdU-labeling method. (biomedsearch.com)
  • In the absence of BTG1, the stem and progenitor cells initially hyper proliferate and then in the longer period lose the ability to proliferate and expand. (wikipedia.org)
  • the role of BTG2, however, appears to differ from that of BTG1 being probably more relevant in controlling the terminal differentiation of neural stem and progenitor cells in the adult neurogenic niches. (wikipedia.org)
  • neuronal
  • In this study, we examined the effects of PS1 overexpression on neuronal morphology using SH-SY5Y cells. (biomedsearch.com)
  • Angiographic findings suggest that central neurocytoma (CN) might originate from neuronal cells of the subventricular zone (SVZ) around the foramen of Monro rather than from the septum pellucidum. (biomedsearch.com)
  • In this study we have investigated the impact of differentiation of neuronal cells on their sensitivity to microbial toxins. (biomedsearch.com)
  • malignant
  • This finding often create alarm and anxiety, because it has to be placed in a differential diagnosis versus low-stage malignant germ cell tumors. (clinicaltrials.gov)
  • Given that the focal areas of Leydig cell hyperplasia are nowadays easily detectable at ultrasonography of the testis (US), as small non-palpable hypoechoic micro-nodules that can show internal vascularization, their finding create a diagnostic challenge versus low-stage malignant germ cell tumors. (clinicaltrials.gov)
  • selectively
  • 6-Hydroxydopamine (6-OHDA) is often used in models of Parkinson's disease since it can selectively target and kill dopaminergic cells of the substantia nigra. (biomedsearch.com)
  • mitotic cyclins
  • Cdh1 plays a pivotal role in controlling cell division at the end of mitosis (telophase) and in the subsequent G1 phase of cell cycle: By recognizing and binding proteins (like mitotic cyclins) which contain a destruction box (D-box) and an additional degradation signal (KEN box), Cdh1 recruits them in a C-box-dependent mechanism to the APC for ubiquination and subsequent proteolysis. (wikipedia.org)
  • The main function of Cdh1 is to suppress the re-accumulation of mitotic cyclins and other cell cycle determinants and therefore stabilising the G1-phase. (wikipedia.org)
  • APCCdh1 is thought to prevent premature S-phase entry by degrading mitotic cyclins in G1 and regulate processes unrelated to the cell cycle. (wikipedia.org)
  • assay
  • The cell proliferation assay is an important tool in the assessment of. (bio-medicine.org)
  • The cell proliferation assay is an important tool in the assessment of the immune system function and in the search for new drugs. (bio-medicine.org)
  • Results from a cell proliferation assay are presented which compare the performance of TopCount to the traditional counting method. (bio-medicine.org)
  • The fluorochrome-based TUNEL assay applicable for flow cytometry, combining the detection of DNA strand breaks with respect to the cell cycle-phase position, was originally developed by Gorczyca et al. (wikipedia.org)
  • However, for years there has been a debate about its accuracy, due to problems in the original assay which caused necrotic cells to be inappropriately labeled as apoptotic. (wikipedia.org)
  • 1992
  • Since 1992 the TUNEL has become one of the main methods for detecting apoptotic programmed cell death. (wikipedia.org)
  • donor
  • Somatic cell nuclear transfer (SCNT) using genetically modified donor cells facilitates the generation of tailored pig models for biomedical research and for xenotransplantation. (biomedsearch.com)
  • cellular
  • This 3-week-long series of collaborative laboratory exercises explores how to use a cultured cell system (PC12 cells) to study signaling pathways involved in cellular differentiation. (biomedsearch.com)
  • The device enables real-time analysis of cellular and tissue responses to oxygen tension to define how cells adapt to heterogeneous oxygen conditions found in the physiological setting. (biomedsearch.com)
  • The protection of granulosa cells from cellular injury caused by oxidative stress is an effective therapy for female infertility. (biomedsearch.com)
  • The assessment of cellular half-life is of fundamental importance for cell biology and biomedicine. (biomedsearch.com)
  • receptors
  • Sorafenib and sutinib are additional angiogenesis inhibitors that bind and block receptors on endothelial cells that have important roles in downstream pathways contributing to angiogenesis progression. (wikipedia.org)
  • Cholesterol is imported into the neuron by apolipoprotein E (apoE) via LRP1 receptors on the cell surface. (wikipedia.org)
  • CNTs can be internalized by cells, first by binding their tips to cell membrane receptors. (wikipedia.org)
  • pathways
  • Development of mitochondrially-targeted drugs is receiving increasing attention because of the central roles these organelles play in energy production, reactive oxygen generation, and regulation of cell death pathways. (biomedsearch.com)
  • induction
  • Induction of beta-cell proliferation by either 5-IT or harmine, another natural-product DYRK1A inhibitor, was suppressed by co-incubation with the calcineurin inhibitor FK506, suggesting involvement of DYRK1A and NFAT signaling. (diabetesjournals.org)
  • METHODS: WJCs subjected to adipocyte induction were observed changes in the cell morphology and alterations in actin filament formation. (biomedsearch.com)
  • dependent
  • The serum- and glucocorticoid-dependent kinases-1-3 (SGK1-3) are downstream effectors of PI3 kinases, implicated in various cell responses including colon cancer tumorigenesis in mice. (biomedsearch.com)
  • p21(Waf1/Cip1/Sdi1) is a cyclin-dependent kinase inhibitor that mediates cell cycle arrest. (biomedsearch.com)
  • Trogocytosis is a cell-contact dependent intercellular transfer of membrane fragments and associated molecules. (biomedsearch.com)
  • OBJECTIVE: Unresponsiveness to chemotherapy is a major problem in the treatment of leukemia, which can be caused by unresponsiveness of noncycling cells to cell cycle-dependent cytotoxic agents. (biomedsearch.com)
  • yeast
  • One characteristic of budding yeast cells exit from mitosis after chromosome segregation is the removal of the mitotic determinants. (wikipedia.org)
  • gene
  • The BTG1 gene locus has been shown to be involved in a t(8;12)(q24;q22) chromosomal translocation in a case of B-cell chronic lymphocytic leukemia. (wikipedia.org)
  • proliferative
  • However, in the absence of secondary stressors, BrdU is thought to substitute relatively benignly for thymidine and is commonly used to 'birth-date' proliferative cells. (biomedsearch.com)
  • In breast cancer Ki67 identifies a high proliferative subset of patients with ER-positive breast cancer who derive greater benefit from adjuvant chemotherapy Ki-67 is an excellent marker to determine the growth fraction of a given cell population. (wikipedia.org)
  • neural
  • The epidermal or neural fate of a proneural cell depends on the concentrations of proteins of the achaete-scute complex. (biomedsearch.com)
  • The proliferating cells are in the ventricular zone in the neural tube and therefore colored red. (wikipedia.org)
  • kinase
  • Kinome profiling revealed 5-IT to be a potent and selective inhibitor of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) and cell division cycle (CDC)-like (CLK) kinase families. (diabetesjournals.org)
  • growth
  • A convenient system used to measure cell growth and drug sensitivity in tumor cell lines. (atcc.org)
  • The term cell growth is used in the contexts of cell development and cell division (reproduction). (medicalxpress.com)
  • Rather, the gp130 mutation caused nonhematopoietic cells to produce excess IL-7-a growth factor that triggers T cell proliferation. (rupress.org)
  • The latter can work as a growth factor for Leydig cells. (clinicaltrials.gov)
  • The growth of a plant cell encompasses a complex set of subcellular components interacting in a highly coordinated fashion. (biomedsearch.com)
  • Vertebrate embryos are characterized by an elongated antero-posterior (AP) body axis, which forms by progressive cell deposition from a posterior growth zone in the embryo. (biomedsearch.com)
  • Granulosa cells form ovarian follicles and play important roles in the growth and maturation of oocytes. (biomedsearch.com)
  • However, there are now multiple studies that illustrate the complexity behind these previous simple conceptions of angiogenesis, indicating that the way cancer cells interact with and co-opt new blood vessel growth varies amongst cancer types and must be studied. (wikipedia.org)
  • proteins
  • The mechanical properties of extracellular matrix proteins strongly influence cell-induced tension in the matrix, which in turn influences cell function. (biomedsearch.com)
  • detection
  • The Universal Mycoplasma Detection Kit, ATCC 30-1012K , offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. (atcc.org)
  • Erythrophore cell response to food-associated pathogenic bacteria: implications for detection. (biomedsearch.com)
  • We report the use of Betta splendens chromatophore cells, specifically erythrophore cells, for detection of food-associated pathogenic bacteria. (biomedsearch.com)