• 1995
  • The p14ARF transcript was first identified in humans in 1995, and its protein product confirmed in mice that same year. (wikipedia.org)
  • Genes
  • CDK-dependent pRb hyperphosphorylation releases E2F transcription factors, thus contributing to the expression of several growth and cell cycle-regulatory genes with functional E2F-binding sites in their promoters. (ahajournals.org)
  • It is located near the genes for the tandem repeats INK4a and INK4b, which are 16 kDa (p16INK4a) and 15 kDa (p15INK4b) proteins, respectively. (wikipedia.org)
  • The Rb protein limits cell proliferation by blocking the activity of E2F transcription factors, which activate the transcription of genes needed for DNA replication. (wikipedia.org)
  • RbAp48
  • The results were consistent with the human studies, where the level of RbAp48 protein was much lower in adults as compared to the young. (wikipedia.org)
  • Recombinant protein encompassing a sequence within the center region of human RbAp48. (genetex.com)
  • SH-SY5Y whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [C1C(GTX114707) diluted at 1:500. (genetex.com)
  • RbAp48 antibody [N1Cdetects RbAp48 protein at nucleus by immunofluorescent analysis. (genetex.com)
  • Green: RbAp48 protein stained by RbAp48 antibody [N1C(GTX115554) diluted at 1:500. (genetex.com)
  • cycle progression
  • Eliminating RB function has been shown to allow unregulated cell cycle progression and promotes tumour growth. (ebi.ac.uk)
  • Although the INK4a and ARF proteins are structurally and functionally different, they are both involved in cell cycle progression. (wikipedia.org)
  • On one end, the hypermethylation, mutation, or deletion of p16 leads to downregulation of the gene and can lead to cancer through the dysregulation of cell cycle progression. (wikipedia.org)
  • complexes
  • The PSF (polypyrimidine tract-binding protein-associated splicing factor) within the PER complex recruits SIN3A, a scaffold for assembly of transcriptional inhibitory complexes and rhythmically delivers histone deacetylases to the Per1 promoter, which repress Per1 transcription. (wikipedia.org)
  • This encoded protein is also part of corepressor complexes, which is an integral component of transcriptional silencing. (wikipedia.org)
  • Access to nucleosomal DNA is governed by two major classes of protein complexes: Covalent histone-modifying complexes. (wikipedia.org)
  • Specific protein complexes, known as histone-modifying complexes catalyze addition or removal of various chemical elements on histones. (wikipedia.org)
  • This protein complexes with transcriptional co-repressor CTBP. (wikipedia.org)
  • The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. (wikipedia.org)
  • This gene is present in many protein complexes that are involved in histone acetylation and deacetylation processes as well as chromatin assembly. (wikipedia.org)
  • This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. (genetex.com)
  • PRC1 and PRC2 are two protein complexes that modify the expression of p16 through the interaction of various transcription factors that execute methylation patterns that can repress transcription of p16. (wikipedia.org)
  • progression
  • p16 plays an important role in cell cycle regulation by decelerating cells progression from G1 phase to S phase, and therefore acts as a tumor suppressor that is implicated in the prevention of cancers, notably melanoma, oropharyngeal squamous cell carcinoma, cervical cancer, and esophageal cancer. (wikipedia.org)
  • In spite of their structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in control of the G1 phase of the cell cycle. (wikipedia.org)
  • adenovirus
  • Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 Large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. (wikipedia.org)
  • 1 ) HGF overexpression and enhanced β-cell engraftment were not associated with increases in β-cell proliferation, 2 ) abundant adenovirus expression persisted the full 6 weeks of the experiment, and 3 ) murine HGF was superior to human HGF (hHGF) in this system. (diabetesjournals.org)
  • tumor cells
  • In human tumor cells, VEGF-D colocalized with eIF4GI and 4E-BP1, which can program increased initiation at IRES motifs on mRNA by the translational initiation complex. (ac.be)
  • We found that myofibroblasts triggered cues in the tumor microenvironment, which led to induction of CXCL4L1 in tumor cells. (ac.be)
  • transgenic mice
  • Hepatocyte growth factor overexpression in the islet of transgenic mice increases beta cell proliferation, enhances islet mass, and induces mild hypoglycemia. (semanticscholar.org)
  • As a result of these events, HGF-overexpressing islets sense glucose and secrete insulin in a fashion superior to normal islets ( 3 , 4 ), and β-cell-targeted HGF transgenic mice develop mild hypoglycemia ( 3 , 4 ). (diabetesjournals.org)
  • variants encoding
  • At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4. (wikipedia.org)
  • Mdm2
  • Mdm2 thus appears to be an essential p53 antagonist in the developing embryo and in mature differentiated cells. (biologists.org)
  • Loss of p14ARF by a homozygous mutation in the CDKN2A (INK4A) gene will lead to elevated levels in mdm2 and, therefore, loss of p53 function and cell cycle control. (wikipedia.org)