• RbAp48
  • Recombinant protein encompassing a sequence within the center region of human RbAp48. (genetex.com)
  • SH-SY5Y whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [C1C(GTX114707) diluted at 1:500. (genetex.com)
  • RbAp48 antibody [N1Cdetects RbAp48 protein at nucleus by immunofluorescent analysis. (genetex.com)
  • Green: RbAp48 protein stained by RbAp48 antibody [N1C(GTX115554) diluted at 1:500. (genetex.com)
  • The results were consistent with the human studies, where the level of RbAp48 protein was much lower in adults as compared to the young. (wikipedia.org)
  • cyclin
  • Cyclin D1 can stimulate proliferation by driving cells from the G1 into the S-phase of the mammalian cell cycle. (semanticscholar.org)
  • Previous studies have demonstrated a protective effect of the cyclin-dependent kinase (CDK) inhibitor p27 Kip1 against atherosclerosis and restenosis, two disorders characterized by abundant proliferation and migration of vascular smooth muscle cells and adventitial fibroblasts. (ahajournals.org)
  • Cell cycle progression is controlled by several cyclin-dependent kinases (CDKs) that associate with regulatory cyclins. (ahajournals.org)
  • 3 Mitogenic stimuli activate CDK/cyclin holoenzymes, thus causing hyperphosphorylation of the retinoblastoma protein (pRb) and related "pocket" proteins from mid G 1 to mitosis. (ahajournals.org)
  • Abemaciclib (LY2835219) is an investigational, oral cell cycle inhibitor, designed to block the growth of cancer cells by specifically inhibiting cyclin-dependent kinases, CDK 4 and CDK 6 and was most active against Cyclin D1 and CDK 4 in cell-free enzymatic assays. (salesandmarketingnetwork.com)
  • p14ARF inhibits mdm2, thus promoting p53, which promotes p21 activation, which then binds and inactivates certain cyclin-CDK complexes, which would otherwise promote transcription of genes that would carry the cell through the G1/S checkpoint of the cell cycle. (wikipedia.org)
  • An important cyclin-dependent substrate is the retinoblastoma protein Rb, which is phosphorylated in late gap 1 phase (G1 phase), allowing G1 exit. (wikipedia.org)
  • When Rb is phosphorylated by cyclin D and E-dependent kinases during the G1 phase of the cell cycle, Rb can not block E2F-dependent transcription, and the cell can progress to the DNA synthetic phase(S phase). (wikipedia.org)
  • This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. (wikipedia.org)
  • p16 (also known as cyclin-dependent kinase inhibitor 2A, multiple tumor suppressor 1 and as several other synonyms), is a tumor suppressor protein, that in humans is encoded by the CDKN2A gene. (wikipedia.org)
  • p16 is a cyclin-dependent kinase (CDK) inhibitor that slows down the cell cycle by prohibiting progression from G1 phase to S phase. (wikipedia.org)
  • Normally, CDK4/6 binds cyclin D and forms an active protein complex that phosphorylates retinoblastoma protein (pRB). (wikipedia.org)
  • p16 acts as a tumor suppressor by binding to CDK4/6 and preventing its interaction with cyclin D. This interaction ultimately inhibits the downstream activities of transcription factors, such as E2F1, and arrests cell proliferation. (wikipedia.org)
  • transcription factors
  • Histone deacetylase inhibitors exert their anti-tumour effects via the induction of expression changes of oncogenes or tumour suppressor, through modulating that the acetylation/deactylation of histones and/or non-histone proteins such as transcription factors. (wikipedia.org)
  • Non-histone proteins such as transcription factors are also targets for acetylation with varying functional effects. (wikipedia.org)
  • Pubmed ID: 11823797 Many transcription factors in human cells have functional orthologues in yeast, and a common experimental theme has been to define the function of the yeast protein and then test whether the mammalian version behaves similarly. (jove.com)
  • CDK-dependent pRb hyperphosphorylation releases E2F transcription factors, thus contributing to the expression of several growth and cell cycle-regulatory genes with functional E2F-binding sites in their promoters. (ahajournals.org)
  • The Rb protein limits cell proliferation by blocking the activity of E2F transcription factors, which activate the transcription of genes needed for DNA replication. (wikipedia.org)
  • As mentioned above, INK4a inhibits proliferation by indirectly allowing Rb to remain associated with E2F transcription factors. (wikipedia.org)
  • The protein encoded by this gene is a member of the E2F family of transcription factors. (wikipedia.org)
  • degradation
  • p14ARF is an atypical protein, in terms of its transcription, its amino acid composition, and its degradation: it is transcribed in an alternate reading frame of a different protein, it is highly basic, and it is polyubiquinated at the N-terminus. (wikipedia.org)
  • Mdm2 also has E3 ubiquitin ligase activity toward p53, and promotes its exportation from the cell nucleus to the cytoplasm for degradation. (wikipedia.org)
  • inhibits
  • We show here that p27 Kip1 inhibits cellular changes that normally occur during cell locomotion (eg, lamellipodia formation and reorganization of actin filaments and focal adhesions). (ahajournals.org)
  • adenovirus
  • 1 ) HGF overexpression and enhanced β-cell engraftment were not associated with increases in β-cell proliferation, 2 ) abundant adenovirus expression persisted the full 6 weeks of the experiment, and 3 ) murine HGF was superior to human HGF (hHGF) in this system. (diabetesjournals.org)
  • Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 Large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. (wikipedia.org)
  • tumor cells
  • In human tumor cells, VEGF-D colocalized with eIF4GI and 4E-BP1, which can program increased initiation at IRES motifs on mRNA by the translational initiation complex. (ac.be)
  • We found that myofibroblasts triggered cues in the tumor microenvironment, which led to induction of CXCL4L1 in tumor cells. (ac.be)
  • cycle progression
  • Eliminating RB function has been shown to allow unregulated cell cycle progression and promotes tumour growth. (ebi.ac.uk)
  • Although the INK4a and ARF proteins are structurally and functionally different, they are both involved in cell cycle progression. (wikipedia.org)
  • On one end, the hypermethylation, mutation, or deletion of p16 leads to downregulation of the gene and can lead to cancer through the dysregulation of cell cycle progression. (wikipedia.org)
  • nuclear
  • NPM is an acidic ribosomal chaperone (protein) involved in preribosomal processing and nuclear exportation independent of p53, and oligomerizes with itself and p14ARF. (wikipedia.org)
  • transgenic mice
  • Hepatocyte growth factor overexpression in the islet of transgenic mice increases beta cell proliferation, enhances islet mass, and induces mild hypoglycemia. (semanticscholar.org)
  • As a result of these events, HGF-overexpressing islets sense glucose and secrete insulin in a fashion superior to normal islets ( 3 , 4 ), and β-cell-targeted HGF transgenic mice develop mild hypoglycemia ( 3 , 4 ). (diabetesjournals.org)
  • progression
  • In spite of their structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in control of the G1 phase of the cell cycle. (wikipedia.org)
  • variants encoding
  • At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4. (wikipedia.org)
  • cancers
  • A research published by "Nature" in 01/2016, authored by Italian researchers Antonio Iavarone and Anna Lasorella, from Columbia University, states that ID2 protein has a relevant role in the development and resistance to therapies of glioblastoma, the most aggressive of brain cancers. (wikipedia.org)
  • Mutations resulting in deletion or reduction of function of the CDKN2A gene are associated with increased risk of a wide range of cancers and alterations of the gene are frequently seen in cancer cell lines. (wikipedia.org)