• types
  • Through careful optimization--e.g. choosing the right transfection agent and transfection method--high levels of transfection efficiency can be achieved in many cell types. (bio-medicine.org)
  • Both siPORT NeoFX and siPORT Amine Transfection Agents can be used for reverse transfection or standard transfection of siRNAs into a wide variety of cell types. (bio-medicine.org)
  • Ambion's siRNA Delivery Resource has further details on transfection optimization, recommendations for transfection agent, and conditions to use with specific cell types. (bio-medicine.org)
  • high
  • When radioiodinated lymphocytes were solubilized with Nonidet P-40, 3-4% of radioiodinated high-molecular-weight protein of B-cells consisted of immunoglobulin, a result similar to that found with acid-urea extraction. (biochemj.org)
  • particular
  • Once a protocol is optimized for a particular cell type, reproducible siRNA screening experiments can easily be done. (bio-medicine.org)
  • The goal of transfection optimization is to determine the conditions that will provide maximum gene knockdown while maintaining an acceptable level of viability for the particular cell type (see sidebar, Two-Step Optimization Protocol ). (bio-medicine.org)
  • samples
  • CardioTACS™ In Situ Apoptosis Detection Kits Ordering Information ORDER ONLINE 4827-30-K CardioTACS Kit, 30 Samples $285 The CardioTACS Kit was developed to provide the heart researcher with an effective method for identifying apoptotic cells in cardiac samples. (selectscience.net)
  • activity
  • In a second xenograft study using A549 lung cancer cells and varied schedules of dosing, activity of peloruside was again superior compared with the taxanes with inhibitions ranging from 51% to 74%, compared with 44% and 50% for the two taxanes. (aacrjournals.org)
  • surface
  • Accessible surface proteins of thymus-derived lymphocytes (T-cells) of normal CBA mice and bone-marrow-derived lymphocytes (B-cells) of congenitally athymic nu/nu mice were analysed. (biochemj.org)
  • However, with the detergent extraction, only 0.1% of T-cell surface protein was precipitable by anti-globulin reagents. (biochemj.org)
  • These results provide further evidence that the surface membranes of normal T-cells and B-cells differ in physicochemical properties. (biochemj.org)