Loading...
  • genes
  • The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes, and developmentally important genes such as the polycomb group of genes. (wikipedia.org)
  • YPEL3 was discovered to be a possible p53 target after a screen for such genes was performed in MCF7 breast cancer cells following RNAi knockdown of p53 negative inhibitors. (wikipedia.org)
  • As a part of the p53 pathway response and its anti-proliferation role, cellular senescence has gained attention for its working relationship with tumor suppressor genes. (wikipedia.org)
  • The predominant allele has a normal, tumor suppressive function whereas high penetrance mutations in these genes cause a loss of tumor suppressive function which correlates with an increased risk of breast cancer. (wikipedia.org)
  • proteins
  • All members of the TNF family, with the exception of the secreted lymphotoxin and a proliferation-inducing ligand (APRIL), are type II transmembrane proteins that protrude from immune cells. (wikipedia.org)
  • The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. (wikipedia.org)
  • BRCA1 and BRCA2 are unrelated proteins, but both are normally expressed in the cells of breast and other tissue, where they help repair damaged DNA, or destroy cells if DNA cannot be repaired. (wikipedia.org)
  • murine
  • In the same report, these authors also reported the exquisite anticancer activity of halichondrin B against murine cancer cells both in culture and in in vivo studies. (wikipedia.org)
  • More recently, PEGylated recombinant murine IL-10 (PEG-rMuIL-10) has been shown to induce IFNγ and CD8+ T cell dependent anti-tumor immunity. (wikipedia.org)
  • The GR1 marker is made up of two cell membrane molecules, Ly6C and Ly6G, and according to their relative expression levels murine MDSCs are further classified into two subtypes, monocytic and granulocytic. (wikipedia.org)
  • Human MDSCs are less characterized, and they are generally defined as myeloid cells expressing CD33, CD14 and low levels of HLA DR. The absence of the human equivalent to the murine GR1 marker makes it difficult to compare murine and human MDSCs. (wikipedia.org)
  • Although studies in murine myeloid precursor cell lines indicated YPEL3 to have a role in apoptosis, human YPEL3 failed to demonstrate an apoptotic response using sub-G1 or poly ADP ribose polymerase cleavage as accepted indicators of programmed cell death. (wikipedia.org)
  • inhibit
  • The endothelial growth factor antibodies used did not inhibit or stimulate cell growth when compared to control but did discourage vascular mimicry. (biomedsearch.com)
  • vitro
  • Lymphotoxin-alpha (LT-alpha) and lymphotoxin-beta (LT-beta), two related cytokines produced by lymphocytes that are cytotoxic for a wide range of tumor cells in vitro and in vivo. (wikipedia.org)
  • phosphorylation
  • Analysis of topoisomerase II immunoprecipitates from 32P-labeled HeLa cells indicated that phosphorylation of the enzyme occurred at serine residues. (rti.org)
  • antigen
  • T cell antigen gp39 (CD40L), a cytokine that seems to be important in B-cell development and activation. (wikipedia.org)
  • Discovered in 1991 IL-10 was initially reported to suppress cytokine secretion, antigen presentation and CD4+ T cell activation. (wikipedia.org)
  • MCF7
  • Studies in U2OS osteosarcoma cells and MCF7 breast cancer cells have also demonstrated increased cellular senescence upon YPEL3 induction. (wikipedia.org)
  • ATCC
  • Glioma cell line U87MG was obtained from the American Type Culture Collection (ATCC) (Manassas, VA), and glioma cell line 1321N1 was obtained from the European Collection of Cell Culture (ECACC) (Salisbury, Wiltshire, UK). (hindawi.com)
  • mast cells
  • In humans, IL-10 is encoded by the IL10 gene, which is located on chromosome 1 and comprises 5 exons, and is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. (wikipedia.org)
  • A study in mice has shown that IL-10 is also produced by mast cells, counteracting the inflammatory effect that these cells have at the site of an allergic reaction. (wikipedia.org)
  • however, it is also stimulatory towards certain T cells (Th2) and mast cells and stimulates B cell maturation and antibody production. (wikipedia.org)
  • myeloid lineage
  • Further investigation has shown that IL-10 predominantly inhibits lipopolysaccharide (LPS) and bacterial product mediated induction of the pro-inflammatory cytokines TNFα, IL-1β, IL-12, and IFNγ secretion from Toll-Like Receptor (TLR) triggered myeloid lineage cells. (wikipedia.org)
  • In mouse models, MDSCs are found as myeloid cells expressing high levels of CD11b (a classical myeloid lineage marker) and GR1 (granulocytic marker). (wikipedia.org)
  • function
  • Scientists study the behaviour of isolated cells grown in the laboratory for insights into how cells function in the body in health and disease. (wikipedia.org)
  • immune
  • Such membrane-bound TNF ligands frequently signal back to the immune cells when they contact and bind their cognate receptors on other cells. (wikipedia.org)
  • These MDSCs infiltrate inflammation sites and tumors, where they stop immune responses by inhibiting T cells and NK cells, for example. (wikipedia.org)
  • oncogenic
  • Characterized by the limited ability of cultured normal cells to divide, senescence has been shown to be triggered through oncogenic activation( premature senescence) as well as telomere shortening as the result of successive rounds of DNA replication (replicative senescence). (wikipedia.org)
  • suppressor
  • Our recent study showing reversible regulation of tumor suppressor p53 by Complex I deficiency provides a mechanistic explanation for this (Compton et. (pvlsi.org)
  • While the pathway is very detailed and not completely understood, in general, when Wnt is not present, GSK-3B (a member of the pathway) is able to phosphorylate β-catenin as a result of a complex formation that includes β-catenin, AXIN1, AXIN2, APC (a tumor suppressor gene product), CSNK1A1, and GSK3B. (wikipedia.org)
  • solid tumor
  • In a Phase 1/2 trial, in solid tumor patients refractory to standard therapy, response evaluation showed disease stabilization in a majority of patients. (wikipedia.org)
  • antigens
  • The technique is also used to determine tumour antigens (for use in diagnosis and therapeutic targeting) and in searching for protein-DNA interactions using specially-constructed DNA libraries with randomised segments. (wikipedia.org)
  • tissue
  • They treated the cancer cells with low doses of parabens along with heregulin, a growth-promoting substance that's normally found in breast tissue. (emedicinehealth.com)
  • The new study found that in the presence of growth-promoting substances that are normally in breast tissue, parabens can promote the growth of estrogen-sensitive breast tumors even in tiny amounts -- around 5 to 10 nanograms. (emedicinehealth.com)
  • Finally, peritoneal cancer can be caused by a lot of tumors inside or outside the abdominal cavity such as pancreas cancer, biliary cancer , sarcoma (cancer of the connective tissue) and breast cancer . (drmulier.com)
  • Gilmour continued her research at The Wistar Institute in Philadelphia, Pennsylvania, first as postdoctoral fellow (1984 to 1987) and then as research associate (1988 to 1990 While at Wistar, she began her work in characterizing the regulation and expression of ornithine decarboxylase (ODC) in both normal and tumor tissue. (wikipedia.org)
  • The cells of the tumor will often grow into nearby normal brain tissue. (wikipedia.org)
  • It was the first time the FDA had approved a cancer drug based on tumor genetics rather than tissue type or tumor site. (wikipedia.org)
  • metastases
  • Although carcinoid tumors with invasion to the muscular layer increase lymph node metastases [ 5 ], the depth of tumors ≤ 10 mm in diameter is often limited to the submucosal layer [ 6 ]. (omicsonline.org)
  • Although medical resection with adjuvant chemotherapy and/or radiotherapy are accustomed to treat breast tumors, regular cells tolerance, development of metastases, and natural tumor resistance to radiation or chemotherapy can hinder an effective outcome. (biomasswars.com)
  • epithelial
  • Genital warts, which are a form of benign tumor of epithelial cells, are also caused by various strains of HPV. (wikipedia.org)
  • These complexes, which help regulate cell growth in addition to creating and maintaining epithelial layers, are known as adherens junctions and they typically include at least cadherin, β-catenin, and α-catenin. (wikipedia.org)
  • The primary mechanical role of catenins is connecting cadherins to actin filaments, specifically in these adhesion junctions of epithelial cells. (wikipedia.org)
  • For instance, when an epithelial layer is complete and the adherens junctions indicate that the cell is surrounded, β-catenin may play a role in telling the cell to stop proliferating, as there is no room for more cells in the area. (wikipedia.org)
  • genomic
  • All HAP1 knockout cell lines are validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. (horizondiscovery.com)
  • They have developed an approach that uses bioinformatic resources to identify therapeutically actionable genomic alterations in tumors, which is accessible through another online database called the Cancer Genome Interpreter. (wikipedia.org)
  • gene
  • Customers obtain a clonal HAP1 cell line, bearing a frameshift mutation in an exon of the gene of interest. (horizondiscovery.com)
  • The phage gene and insert DNA hybrid is then inserted (a process known as "transduction") into Escherichia coli (E. coli) bacterial cells such as TG1, SS320, ER2738, or XL1-Blue E. coli. (wikipedia.org)
  • This discovery changed the current thinking about cancer from a model wherein cancer is caused by a foreign substance (a viral gene) to one where a gene that is normally present in the cell can cause cancer. (wikipedia.org)
  • widely
  • c-Src is a widely studied member of non-receptor tyrosine kinases which are not associated with a cell-surface receptor. (wikipedia.org)
  • Administration of Type I IFN has been shown experimentally to inhibit tumor growth in animals, but the beneficial action in human tumors has not been widely documented. (wikipedia.org)
  • invasion
  • Additionally, our outcomes also claim that TRPC3 shows up as a fresh mediator of breasts cancer tumor cell migration/invasion and represents a potential focus on for a fresh course of anticancer agent. (cancercurehere.com)
  • myeloid
  • However, counting CD34+ mononuclear cells may overestimate myeloid blasts in bone marrow smears due to hematogones (B lymphocyte precursors) and CD34+ megakaryocytes. (wikipedia.org)
  • squamous
  • Bradykinin (BK) has been proven to promote development and migration of head and neck squamous cell carcinoma (HNSCC) cells via epidermal development factor receptor (EGFR) transactivation. (biomasswars.com)
  • The commonest non -melanoma skin cancers are basal cell carcinoma and squamous cell carcinoma. (garylross.com)
  • Human
  • Two studies have found parabens in human breast tumors. (emedicinehealth.com)
  • Granulysin is a cytolytic and proinflammatory molecule first identified by a screen for genes expressed "late" (3-5 days) after activation of human peripheral blood mononuclear cells. (wikipedia.org)
  • Aminopeptidase N is frequently overexpressed in tumors and has been associated with the growth of different human cancers suggesting it as a suitable target for anti-cancerous therapy. (wikipedia.org)
  • Pembrolizumab is an immunoglobulin G4, with a variable region against the human programmed cell death 1 receptor, a humanized mouse monoclonal [228-L-proline(H10-S>P)]γ4 heavy chain (134-218') disulfide and a humanized mouse monoclonal κ light chain dimer (226-226:229-229)-bisdisulfide. (wikipedia.org)
  • inflammatory
  • 9kD granulysin is also a chemoattractant for T lymphocytes, monocytes, and other inflammatory cells and activates the expression of a number of cytokines, including RANTES, MCP-1, MCP-3, MIP-1α, IL-10, IL-1, IL-6 and IFNα. (wikipedia.org)
  • A negative CD34 may exclude Ewing's sarcoma/PNET, myofibrosarcoma of the breast, and inflammatory myofibroblastic tumors of the stomach. (wikipedia.org)
  • peripheral
  • Injection of CD34+ hematopoietic Stem Cells has been clinically applied to treat various diseases including Spinal Cord Injury, Liver Cirrhosis and Peripheral Vascular disease. (wikipedia.org)
  • receptor
  • Interferon type I: All type I IFNs bind to a specific cell surface receptor complex known as the IFN-α/β receptor (IFNAR) that consists of IFNAR1 and IFNAR2 chains. (wikipedia.org)
  • It targets the programmed cell death 1 (PD-1) receptor of lymphocytes. (wikipedia.org)
  • hematopoietic cells
  • Src, Fyn and Yes are expressed ubiquitously in all cell types while the others are generally found in hematopoietic cells. (wikipedia.org)
  • The presence of CD34 on non-hematopoietic cells in various tissues has been linked to progenitor and adult stem cell phenotypes. (wikipedia.org)
  • It is important to mention that Long-Term Hematopoietic Stem Cells (LT-HSCs) in mice and humans are the hematopoietic cells with the greatest self-renewal capacity. (wikipedia.org)
  • migration
  • Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. (wikipedia.org)
  • Regardless of its mode of action, under all circumstances CD34, and its relatives podocalyxin and endoglycan, facilitates cell migration. (wikipedia.org)
  • stem cells
  • Gilmour provided the first evidence that elevated epidermal levels of polyamines alone can positively affect the recruitment of bulge stem cells in the skin. (wikipedia.org)
  • In the study, reporter mice were used to track stem cells. (wikipedia.org)
  • This finding is significant with regard to the stem cell origin of skin cancer, since carcinogen-targeted stem cells can remain dormant for many years until recruited to develop into a tumor. (wikipedia.org)
  • It may also mediate the attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells. (wikipedia.org)
  • citation needed] CD34 is expressed in roughly 20% of murine hematopoietic stem cells, and can be stimulated and reversed. (wikipedia.org)
  • Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. (wikipedia.org)
  • i.e., they are multipotential hemopoietic stem cells. (wikipedia.org)
  • Receptors
  • Expression of type I and III IFNs can be induced in virtually all cell types upon recognition of viral components, especially nucleic acids, by cytoplasmic and endosomal receptors, whereas type II interferon is induced by cytokines such as IL-12, and its expression is restricted to immune cells such as T cells and NK cells. (wikipedia.org)
  • deficiency
  • Using these models, we are deciphering the mechanisms by which complex I deficiency can confer opposite cell fates in neurodegeneration and cancer. (pvlsi.org)
  • mice
  • Mice lacking plakoglobin have cell adhesion defects in many tissues, although β-catenin substitutes for plakoglobin at many cellular junctions. (wikipedia.org)
  • Cancer Res 46:6246-6429 (1986) Evolution of B cell lineage lymphomas in mice with a retrovirus-induced immunodeficiency syndrome MAIDS. (wikipedia.org)
  • normally
  • F9 embryonal carcinoma cells are similar to the P19 cells shown in Figure 1 and normally have cell-to-cell adhesion mediated by E-cadherin with β-catenin bound to the cytoplasmic domain of E-cadherin. (wikipedia.org)
  • cellular
  • Scientific discoveries: Gilmour's study of polyamines has broadened the scope of medical knowledge about tumor function at the cellular and molecular levels. (wikipedia.org)
  • Genetically
  • F9 cells were genetically engineered to lack β-catenin, resulting in increased association of plakoglobin with E-cadherin. (wikipedia.org)
  • novel
  • Taconic will employ the DuPont OncoMouse® technology to expand its translational oncology research services by performing efficacy and compound profiling studies on behalf of clients and making novel spontaneous tumor mouse models available to investigators for in-house research programs. (andhranews.net)
  • respectively
  • The modification of isoCA-4 by introduction of difluoromethoxy group at the para-position (3i) and substitution of the two protons of the linker by two fluorine atoms (3m), produced the most active compounds in the series, with IC50 values of 0.15-2.2 nM (3i) and 0.1-2 nM (3m) respectively, against a panel of six cancer cell lines. (deepdyve.com)