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  • assay
  • QIAGEN provides a broad range of assay technologies for cell cycle research that enables analysis of gene expression and regulation, epigenetic modification, genotyping, and signal transduction pathway activation. (qiagen.com)
  • The Cignal E2F Reporter Assay Kit is designed to monitor the activity of E2F-regulated signaling pathways in cultured cells. (qiagen.com)
  • ELISA kit, antibody to, antigenes, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. (gentaur.com)
  • interaction
  • Phosphorylation by p38, but not Cdks, triggers an interaction between Rb and the human homologue of murine double minute 2: Hdm2), leading to degradation of Rb, release of E2F1 and cell death. (wustl.edu)
  • Nuclear
  • The Human Anti-Proliferating Cell Nuclear Antigen (PCNA) IgG ELISA kit, 96 tests, Quantitative is manufactured for Research Use Only or for diagnostics purposes. (gentaur.com)
  • cancer
  • Combinations of gemcitabine and trabectedin exert modest synergistic cytotoxic effects on two pancreatic cancer cell lines. (frontiersin.org)
  • PharmaMar S.A.U., Madrid, Spain) is a promising anticancer agent that has demonstrated clinical activity in many drug-resistant cancer cell lines, and has been approved by the US Food and Drug Administration for advanced soft tissue sarcoma. (frontiersin.org)
  • time
  • For example, the G2-M DNA damage checkpoint ensures the fidelity of DNA replication, and arrests the cell cycle to allow time for replication error correction and DNA damage repair. (qiagen.com)
  • Mitosis Under Microscope Lab Report A. The purpose of the investigation is to determine the time an onion cell spends in each part of the cell cycle . (studymode.com)
  • Over time, cells in the control group accumulated in G 0 / G 1 phase. (frontiersin.org)
  • Human
  • The Human Cell Cycle EpiTect Methyl II Signature PCR Array profiles the promoter methylation status of a panel of 22 genes key to cell cycle regulation. (qiagen.com)
  • The Human Cell Cycle EpiTect Chip qPCR Array profiles the histone modification status or "histone code" of 84 genes key to cell cycle regulation. (qiagen.com)
  • ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. (gentaur.com)
  • E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. (gentaur.com)
  • genes
  • A representative sample of cyclical unknown genes (16 total) was epitope tagged in T. gondii tachyzoites yielding the discovery of new protein constituents of the parasite inner membrane complex, key mitotic structures and invasion organelles. (usf.edu)
  • Instead, the outermost cells in these embryos are hyperproliferative and fail to turn on critical epidermal genes. (wikipedia.org)
  • degradation
  • The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. (uniprot.org)
  • Proteins are tagged for degradation with a small protein called ubiquitin. (wikipedia.org)
  • The degradation process yields peptides of about seven to eight amino acids long, which can then be further degraded into shorter amino acid sequences and used in synthesizing new proteins. (wikipedia.org)
  • These α subunits are controlled by binding to "cap" structures or regulatory particles that recognize polyubiquitin tags attached to protein substrates and initiate the degradation process. (wikipedia.org)
  • The importance of proteolytic degradation inside cells and the role of ubiquitin in proteolytic pathways was acknowledged in the award of the 2004 Nobel Prize in Chemistry to Aaron Ciechanover, Avram Hershko and Irwin Rose. (wikipedia.org)
  • Before the discovery of the ubiquitin proteasome system, protein degradation in cells was thought to rely mainly on lysosomes, membrane-bound organelles with acidic and protease-filled interiors that can degrade and then recycle exogenous proteins and aged or damaged organelles. (wikipedia.org)
  • However, work by Alfred Goldberg in 1977 on ATP-dependent protein degradation in reticulocytes, which lack lysosomes, suggested the presence of a second intracellular degradation mechanism. (wikipedia.org)
  • It was then discovered that a previously identified protein associated with proteolytic degradation, known as ATP-dependent proteolysis factor 1 (APF-1), was the same protein as ubiquitin. (wikipedia.org)
  • Later, the ATP-dependent proteolytic complex that was responsible for ubiquitin-dependent protein degradation was discovered and was called the 26S proteasome. (wikipedia.org)
  • IKK-α and IKK-β phosphorylate the IκB proteins, marking them for degradation via ubiquitination and allowing NF-κB transcription factors to go into the nucleus. (wikipedia.org)
  • Mammalian
  • p70 S6 kinase, a target of PI 3-kinase and the mammalian target of rapamycin, was rapidly activated on growth factor stimulation of quiescent coronary artery smooth muscle cells and after balloon injury of rat carotid arteries. (ahajournals.org)
  • The complex functions upstream of ATM in mammalian cells and induces conformational changes that facilitate an increase in the affinity of ATM towards its substrates, such as CHK2 and p53. (wikipedia.org)
  • apoptosis
  • Inhibiting cyclin D3 in the T-ALL leukemia mice caused the cancer cells to self-destruct -- a programmed death process called apoptosis. (medindia.net)
  • residues
  • The fiber protein also contains an HI-loop structure, which can tolerate peptide insertions of up to 100 residues without any negative effects on adenovirus integrity. (wikipedia.org)
  • cellular
  • The most commonly used group of adenoviruses is serotype 5 (Ad5), whose binding to host cells is initiated by interactions between the cellular coxsackie virus and adenovirus receptor (CAR), and the knob domain of the adenovirus coat protein trimer. (wikipedia.org)
  • Cdc14
  • Cdc14 was defined by Hartwell in his famous screen for loci that control the cell cycle of Saccharomyces cerevisiae. (wikipedia.org)
  • Cdc14 was later shown to encode a protein phosphatase. (wikipedia.org)
  • In Saccharomyces cerevisiae, the species in which Cdc14 activity is best understood and most-studied, the activity of Cdc14 (ScCdc14) leads to mitotic exit by dephosphorylating targets of Cdk1, a well-studied cyclin-dependent protein kinase. (wikipedia.org)
  • Additional data in support of this theory was later obtained from studies in zebrafish, where its Cdc14 proteins were also found to localize to the basal body and play roles in the formation of cilia, which are short forms of flagella. (wikipedia.org)
  • Saccharomyces
  • For example, Leucosporidium frigidum grows at −2 to 20 °C (28 to 68 °F), Saccharomyces telluris at 5 to 35 °C (41 to 95 °F), and Candida slooffi at 28 to 45 °C (82 to 113 °F). The cells can survive freezing under certain conditions, with viability decreasing over time. (wikipedia.org)
  • novel
  • Butler, Carrie, "Novel Cell Cycle Proteins In Apicomplexan Parasites" (2014). (usf.edu)
  • The main research goals are to analyse the function and interactions of cancer-related proteins and to develop novel computational methods to analyse, represent and interpret cancer genome information. (wikipedia.org)
  • The central role of Cdc25s in the cell cycle has garnered them considerable attention from the pharmaceutical industry as potential targets for novel chemotherapeutic (anti-cancer) agents. (wikipedia.org)
  • Although some progress has been made in developing potent and selective inhibitors for Cdc25 family of proteins, there is scope for development of novel therapeutic strategies to target them. (wikipedia.org)
  • human
  • In one experiment, human T-ALL cells were infused into mice that then developed the disease. (medindia.net)
  • In addition to these tests with mouse cancers, the scientists found that the cyclin-D-inhibiting drug had similar effects on human blood cancer cells in the laboratory. (medindia.net)
  • Researchers from Germany and the United States describe an important step in how these NETs are released and how they stop a fungus from establishing an infection in mice and human cells in the journal Developmental Cell . (eurekalert.org)
  • Then, they observed human brains with fungal infections and confirmed that our neutrophils are also using cell cycle proteins. (eurekalert.org)
  • In this study, we examined the anti-cancer effect of mimosine on human H226 lung cancer cells grown in nude mice. (spandidos-publications.com)
  • Researchers have used it to gather information about the biology of the eukaryotic cell and ultimately human biology. (wikipedia.org)
  • The crystal structure of human cyclin B". Cell Cycle. (wikipedia.org)
  • Most human cells are produced by mitotic cell division. (wikipedia.org)
  • Decreased IKK-α activity has been reported in a large percentage of human squamous cell carcinomas, and restoring IKK-α in mouse models of skin cancer has been shown to have an anti-tumorigenic effect. (wikipedia.org)
  • genetically
  • Even though the investigators genetically silenced the proteins or blocked them with a drug in normal as well as cancerous tissues, the animals remained healthy, they report in the Oct. 16 issue of the journal Cancer Cell . (medindia.net)
  • Coat-protein modification This method involves genetically modifying the fiber knob domain of the viral coat protein to alter its specificity. (wikipedia.org)
  • encode
  • 4 We hypothesized that in addition to the generalized increases in protein synthesis necessary for cell growth, there is a highly regulated increase in the translation of certain mRNA species, including those that encode cell cycle proteins, and we also hypothesized that the molecular machinery regulating this translation represents a potential target for therapeutic intervention. (ahajournals.org)
  • functional
  • It has also been observed that decreasing cyclin B levels in cells increases the levels of functional p53. (wikipedia.org)
  • Although the highly conserved nature of the Cdc25s implies an important role in cell physiology, Cdc25B and Cdc25C knockout mice (both single and double mutants) are viable and display no major alterations in their cell cycles, suggesting some functional compensation either via other Cdk regulatory enzymes (such as Wee1 and Myt1) or from the activity of the third member of the family, Cdc25A. (wikipedia.org)
  • division
  • Zychlinsky, Amulic, and colleagues hypothesized that neutrophils were using the same cell cycle proteins used for cell division to release the NETs. (eurekalert.org)
  • When cyclin B levels are elevated, cells can enter M phase prematurely and strict control over cell division is lost, which is a favorable condition for cancer development. (wikipedia.org)
  • Cell division was first discovered by the German botanist Hugo von Mohl in 1835 as he worked with the green alga Cladophora glomerata. (wikipedia.org)
  • It alternates with the much longer interphase, where the cell prepares itself for the process of cell division. (wikipedia.org)
  • arrest
  • Activated CHK2 phosphorylates phosphatase CDC25A, which is degraded thereupon and can no longer dephosphorylate CDK2-Cyclin, resulting in cell-cycle arrest. (wikipedia.org)
  • replicative
  • A conditionally replicative adenovirus (CRAd) with a 24 base pair deletion in the retinoblastoma-binding domain of the E1A protein (Ad5- Δ24E3), is unable to silence retinoblastoma, and therefore unable to induce S-phase in host cells. (wikipedia.org)
  • inhibitor
  • The new results shown that the cancers' addiction to these proteins is an Achilles' heel that can be safely targeted with an inhibitor drug that halts cancer growth or causes cancer cells to die. (medindia.net)
  • In contrast to findings in other cell types, growth factor- or balloon injury-induced downregulation of the cell cycle inhibitor p27 Kip1 was not affected by rapamycin treatment. (ahajournals.org)
  • pathway
  • Others were modified to develop a type of T-cell acute lymphoblastic leukemia (T-ALL) that is driven by an abnormal pathway known as Notch1. (medindia.net)
  • section describes the metabolic pathway(s) associated with a protein. (uniprot.org)
  • This protein is involved in the pathway protein ubiquitination, which is part of Protein modification. (uniprot.org)
  • Different domains of the protein are required for this function of IKK-α in zebrafish than in mice, but in neither case does the NF-κB pathway seem to be implicated. (wikipedia.org)
  • Genome
  • The genome is composed of a number of chromosomes-complexes of tightly coiled DNA that contain genetic information vital for proper cell function. (wikipedia.org)
  • complex
  • The proteolytic activities of this system was isolated as a multi-protein complex originally called the multi-catalytic proteinase complex by Sherwin Wilk and Marion Orlowski. (wikipedia.org)
  • IKK-α is a member of the serine/threonine protein kinase family and forms a complex in the cell with IKK-β and NEMO. (wikipedia.org)
  • A complex of the three proteins MRE11, RAD50 and NBS1 (XRS2 in yeast), called the MRN complex in humans, recruits ATM to double strand breaks (DSBs) and holds the two ends together. (wikipedia.org)
  • assay
  • QIAGEN provides a broad range of assay technologies for cell cycle research that enables analysis of gene expression and regulation, epigenetic modification, genotyping, and signal transduction pathway activation. (qiagen.com)
  • The Cignal E2F Reporter Assay Kit is designed to monitor the activity of E2F-regulated signaling pathways in cultured cells. (qiagen.com)
  • ELISA kit, antibody to, antigenes, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. (gentaur.com)
  • interaction
  • Phosphorylation by p38, but not Cdks, triggers an interaction between Rb and the human homologue of murine double minute 2: Hdm2), leading to degradation of Rb, release of E2F1 and cell death. (wustl.edu)
  • Nuclear
  • The Human Anti-Proliferating Cell Nuclear Antigen (PCNA) IgG ELISA kit, 96 tests, Quantitative is manufactured for Research Use Only or for diagnostics purposes. (gentaur.com)
  • cancer
  • Combinations of gemcitabine and trabectedin exert modest synergistic cytotoxic effects on two pancreatic cancer cell lines. (frontiersin.org)
  • PharmaMar S.A.U., Madrid, Spain) is a promising anticancer agent that has demonstrated clinical activity in many drug-resistant cancer cell lines, and has been approved by the US Food and Drug Administration for advanced soft tissue sarcoma. (frontiersin.org)
  • time
  • For example, the G2-M DNA damage checkpoint ensures the fidelity of DNA replication, and arrests the cell cycle to allow time for replication error correction and DNA damage repair. (qiagen.com)
  • Mitosis Under Microscope Lab Report A. The purpose of the investigation is to determine the time an onion cell spends in each part of the cell cycle . (studymode.com)
  • Over time, cells in the control group accumulated in G 0 / G 1 phase. (frontiersin.org)
  • Human
  • The Human Cell Cycle EpiTect Methyl II Signature PCR Array profiles the promoter methylation status of a panel of 22 genes key to cell cycle regulation. (qiagen.com)
  • The Human Cell Cycle EpiTect Chip qPCR Array profiles the histone modification status or "histone code" of 84 genes key to cell cycle regulation. (qiagen.com)
  • ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. (gentaur.com)
  • E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. (gentaur.com)
  • genes
  • Polyamines are ubiquitous small molecules involved in many normal cellular functions, including transcribing and translating genes, regulating ion channels and cell-to-cell interactions, and powering cell growth and replication. (wikipedia.org)
  • Gene
  • In this technique, a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to "display" the protein on its outside while containing the gene for the protein on its inside, resulting in a connection between genotype and phenotype. (wikipedia.org)
  • In the case of M13 filamentous phage display, the DNA encoding the protein or peptide of interest is ligated into the pIII or pVIII gene, encoding either the minor or major coat protein, respectively. (wikipedia.org)
  • The phage gene and insert DNA hybrid is then inserted (a process known as "transduction") into Escherichia coli (E. coli) bacterial cells such as TG1, SS320, ER2738, or XL1-Blue E. coli. (wikipedia.org)
  • A few other mechanisms in E. coli that variously regulate initiation are DDAH (datA-Dependent DnaA Hydrolysis, which is also regulated by IHF), inhibition of the dnaA gene (by the SeqA protein), and reactivation of DnaA by the lipid membrane. (wikipedia.org)
  • Death-associated protein 6 also known as Daxx is a protein that in humans is encoded by the DAXX gene. (wikipedia.org)
  • This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. (wikipedia.org)
  • Other studies showed that lack of Daxx gene caused a higher apoptotic rate in embryonic stem cells. (wikipedia.org)
  • These gene products are important in determining cell fates during normal development and in maintaining homeostasis, or they can lead to de-regulated growth in disorders like cancer by responding to mutations in β-catenin, APC or Axin, each of which can lead to this de-regulated β-catenin level stabilization in cells. (wikipedia.org)
  • Splicing factor, arginine/serine-rich 6 is a protein that in humans is encoded by the SFRS6 gene. (wikipedia.org)
  • The protein encoded by this gene is involved in mRNA splicing and may play a role in site selection in alternative splicing. (wikipedia.org)
  • This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. (wikipedia.org)
  • Abelson murine leukemia viral oncogene homolog 1 also known as ABL1 is a protein that, in humans, is encoded by the ABL1 gene (previous symbol ABL) located on chromosome 9. (wikipedia.org)
  • This new fusion gene, BCR-ABL, encodes an unregulated, cytoplasm-targeted tyrosine kinase that allows the cells to proliferate without being regulated by cytokines. (wikipedia.org)
  • Although senescent cells can no longer replicate, they remain metabolically active and commonly adopt an immunogenic phenotype consisting of a pro-inflammatory secretome, the up-regulation of immune ligands, a pro-survival response, promiscuous gene expression (pGE) and stain positive for senescence-associated β-galactosidase activity. (wikipedia.org)
  • selective
  • Evaluation of cytotoxicity in Human noncancer cells indicated that the compounds 3i and 3m were practically ineffective in quiescent peripheral blood lymphocytes, and may have a selective antiproliferative activity against cancer cells. (deepdyve.com)
  • examples include the influenza A virus M2 proton channel protein, which is highly selective for protons and is active at low pH, and the Chlorella virus Kcv protein, which is selective for potassium ions. (wikipedia.org)
  • membrane
  • Their main structural uses are as part of biological membranes both internal and external, such as the cell membrane, or as a source of energy. (wikipedia.org)
  • Viroporins are capable of assembling into oligomeric ion channels or pores in the host cell's membrane, rendering it more permeable and thus facilitating the exit of virions from the cell. (wikipedia.org)
  • Oligomers of these proteins, most often tetramers, form ion channels or pores of usually weak ion selectivity that permit diffusion of ions across the cell membrane. (wikipedia.org)
  • The molecular architecture of the pore, its degree of selectivity, the extent to which it incorporates lipids from the surrounding membrane, and the presence of portions of the protein that extend beyond the membrane all vary among viroporins and indicate that these proteins have a diverse array of functional roles. (wikipedia.org)
  • The most well-studied and well-established function of viroporins is the permeabilization of the cell membrane to ions and small solutes. (wikipedia.org)
  • An alternative mechanism is illustrated by the SARS-CoV E protein, which forms a pore that integrates membrane lipids whose polar head groups influence ion selectivity. (wikipedia.org)
  • Loss of membrane polarization can promote viral yields through a variety of mechanisms that operate throughout the viral life cycle. (wikipedia.org)
  • In non-enveloped viruses, the membrane permeability changes may be sufficient to induce cell lysis, thereby permitting the new virions to exit the cell. (wikipedia.org)
  • molecular
  • This technology was further developed and improved by groups at the Laboratory of Molecular Biology with Greg Winter and John McCafferty, The Scripps Research Institute with Lerner and Barbas and the German Cancer Research Center with Breitling and Dübel for display of proteins such as antibodies for therapeutic protein engineering. (wikipedia.org)
  • They typically travel in swarms (also known as wolf packs), containing many cells kept together by intercellular molecular signals. (wikipedia.org)
  • Molecular Cell. (wikipedia.org)
  • Cellular
  • Similar life cycles have developed among certain amoebae, called cellular slime molds. (wikipedia.org)
  • The three main purposes of metabolism are the conversion of food/fuel to energy to run cellular processes, the conversion of food/fuel to building blocks for proteins, lipids, nucleic acids, and some carbohydrates, and the elimination of nitrogenous wastes. (wikipedia.org)
  • Metabolism is usually divided into two categories: catabolism, the breaking down of organic matter for example, the breaking down of glucose to pyruvate, by cellular respiration, and anabolism, the building up of components of cells such as proteins and nucleic acids. (wikipedia.org)
  • Amino acids also contribute to cellular energy metabolism by providing a carbon source for entry into the citric acid cycle (tricarboxylic acid cycle), especially when a primary source of energy, such as glucose, is scarce, or when cells undergo metabolic stress. (wikipedia.org)
  • While less attention is directed at α-catenin in studies involving cell adhesion, it is nonetheless an important player in cellular organization, function and growth. (wikipedia.org)
  • Mice lacking plakoglobin have cell adhesion defects in many tissues, although β-catenin substitutes for plakoglobin at many cellular junctions. (wikipedia.org)
  • Cellular senescence is the phenomenon by which normal diploid cells cease to divide. (wikipedia.org)
  • As such, cellular senescence represents a change in "cell state" rather than a cell becoming "aged" as the name confusingly suggests. (wikipedia.org)
  • Consistent with this, telomerase-immortalised cells continued to age (according to the epigenetic clock) without having been treated with any senescence inducers or DNA-damaging agents, re-affirming the independence of the process of epigenetic ageing from telomeres, cellular senescence, and the DNA damage response pathway. (wikipedia.org)
  • Viroporins are small and usually hydrophobic multifunctional viralproteins that modify cellular membranes, thereby facilitating virus release from infected cells. (wikipedia.org)
  • Many viroporins also have additional effects on cellular metabolism and homeostasis mediated by protein-protein interactions with host cell proteins. (wikipedia.org)
  • Viroporins in the membranes of organelles such as the Golgi apparatus can influence those organelles' internal environments, which can modulate protein trafficking of viral proteins or protect the proteins from the low pH they would otherwise encounter in these cellular compartments. (wikipedia.org)
  • Cytoplasmic
  • Daxx, a Death domain-associated protein, was first discovered through its cytoplasmic interaction with the classical death receptor Fas. (wikipedia.org)
  • At the level of the cell, Daxx is found in the cytoplasm, interacting with Fas-receptor or other cytoplasmic molecules, as well as in the nucleus, where it is interacting with some subnuclear structures. (wikipedia.org)
  • F9 embryonal carcinoma cells are similar to the P19 cells shown in Figure 1 and normally have cell-to-cell adhesion mediated by E-cadherin with β-catenin bound to the cytoplasmic domain of E-cadherin. (wikipedia.org)
  • Citric
  • For example, the set of carboxylic acids that are best known as the intermediates in the citric acid cycle are present in all known organisms, being found in species as diverse as the unicellular bacterium Escherichia coli and huge multicellular organisms like elephants. (wikipedia.org)
  • They also code for citrate synthase and two subunits of 2-oxoacid:ferredoxin oxidoreductase, which plays the same role as alpha-ketoglutarate dehydrogenase in the TCA (tricarboxylic/Krebs/citric acid) cycle. (wikipedia.org)
  • vegetative cells
  • Within the fruiting bodies, cells begin as rod-shaped vegetative cells, and develop into rounded myxospores with thick cell walls. (wikipedia.org)
  • Boiling a sample for 30 minutes or more will kill virtually all vegetative cells present, but will not kill spores, which can germinate shortly thereafter and resume growth. (wikipedia.org)
  • This works by killing vegetative cells, allowing germination of surviving spores, and killing the resulting vegetative cells before they have time to form further spores. (wikipedia.org)
  • molecules
  • As these molecules are vital for life, metabolic reactions either focus on making these molecules during the construction of cells and tissues, or by breaking them down and using them as a source of energy, by their digestion. (wikipedia.org)
  • Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells. (wikipedia.org)
  • The BCR-ABL protein can be inhibited by various small molecules. (wikipedia.org)
  • interaction
  • Applications of phage display technology include determination of interaction partners of a protein (which would be used as the immobilised phage "bait" with a DNA library consisting of all coding sequences of a cell, tissue or organism) so that the function or the mechanism of the function of that protein may be determined. (wikipedia.org)
  • Several additional interacting proteins are known, but not always is there an understanding of the specific function and relevance of this interaction. (wikipedia.org)
  • This interaction was demonstrated when PML-NB disrupted cells were treated and Daxx relocalized with the PML-NB. (wikipedia.org)
  • inhibition
  • Meanwhile, several other proteins interact directly with the oriC sequence to regulate initiation, usually by inhibition. (wikipedia.org)
  • β-catenin acts by anchoring the actin cytoskeleton to the junctions, and may possibly aid in contact inhibition signaling within the cell. (wikipedia.org)
  • actin
  • The primary mechanical role of catenins is connecting cadherins to actin filaments, specifically in these adhesion junctions of epithelial cells. (wikipedia.org)
  • interactions
  • Like the two-hybrid system, phage display is used for the high-throughput screening of protein interactions. (wikipedia.org)
  • The technique is also used to determine tumour antigens (for use in diagnosis and therapeutic targeting) and in searching for protein-DNA interactions using specially-constructed DNA libraries with randomised segments. (wikipedia.org)
  • replication
  • Prokaryotic DNA replication is the process by which a prokaryote duplicates its DNA into another copy that is passed on to daughter cells. (wikipedia.org)
  • All cells must finish DNA replication before they can proceed for cell division. (wikipedia.org)
  • Bacterial origins regulate orisome assembly, a nuclei-protein complex assembled on the origin responsible for unwinding the origin and loading all the replication machinery. (wikipedia.org)
  • In E. coli, the direction for orisome assembly are built into a short stretch of nucleotide sequence called as origin of replication (oriC) which contains multiple binding sites for the initiator protein DnaA (a highly homologous protein amongst bacterial kingdom). (wikipedia.org)
  • This was the first time that more than a single origin of DNA replication had been shown to be used in a prokaryotic cell. (wikipedia.org)
  • viroporins are at least partially responsible for this effect, particularly when it occurs late in the viral replication cycle. (wikipedia.org)
  • tumor cell
  • Additionally, our outcomes also claim that TRPC3 shows up as a fresh mediator of breasts cancer tumor cell migration/invasion and represents a potential focus on for a fresh course of anticancer agent. (cancercurehere.com)
  • Within this research, we discovered the functional appearance of TRPC3 in individual MCF-7 breasts cancer tumor cell-mediated Ca2+ entrance. (cancercurehere.com)
  • She discovered that overexpression of ODC and c-Raf activation is sufficient to convert a normal primary keratinocyte into a malignant, invasive tumor cell. (wikipedia.org)
  • coli
  • If a "phagemid" vector is used (a simplified display construct vector) phage particles will not be released from the E. coli cells until they are infected with helper phage, which enables packaging of the phage DNA and assembly of the mature virions with the relevant protein fragment as part of their outer coat on either the minor (pIII) or major (pVIII) coat protein. (wikipedia.org)
  • stem cells
  • Gilmour provided the first evidence that elevated epidermal levels of polyamines alone can positively affect the recruitment of bulge stem cells in the skin. (wikipedia.org)
  • In the study, reporter mice were used to track stem cells. (wikipedia.org)
  • This finding is significant with regard to the stem cell origin of skin cancer, since carcinogen-targeted stem cells can remain dormant for many years until recruited to develop into a tumor. (wikipedia.org)
  • tissues
  • This deterioration is due to the death of the muscle cells and muscle tissues and leads to ongoing muscle wasting and muscle weakness. (blogspot.com)
  • Pasireotide works by binding to a protein called somatostatin receptor, which is expressed in many tissues throughout the body including thyroid cancer cells. (clinicaltrials.gov)
  • adhesion
  • Proteins are also important in cell signaling, immune responses, cell adhesion, active transport across membranes, and the cell cycle. (wikipedia.org)
  • Cell-cell adhesion complexes are required for simple epithelia in higher organisms to maintain structure, function and polarity. (wikipedia.org)
  • Mice engineered to specifically have vascular endothelium cells deficient in β-catenin showed disrupted adhesion between vascular endothelial cells. (wikipedia.org)
  • Keratinocytes engineered to not express alpha-catenin have disrupted cell adhesion and activated NF-κB. (wikipedia.org)
  • bacterial
  • Phage eluted in the final step can be used to infect a suitable bacterial host, from which the phagemids can be collected and the relevant DNA sequence excised and sequenced to identify the relevant, interacting proteins or protein fragments. (wikipedia.org)
  • The use of a helper phage can be eliminated by using 'bacterial packaging cell line' technology. (wikipedia.org)
  • requires the medium to be formulated for this purpose), or the appearance of turbidity (cloudiness indicating light scattering by bacterial cells) indicates that sterilization was not achieved and the sterilization cycle may need revision or improvement. (wikipedia.org)
  • bacteria
  • Media conditions that support fast growth in bacteria also couples with shorter inter-initiation time in them, i.e. the doubling time in fast growing cells is less as compared to the slow growth. (wikipedia.org)
  • receptor
  • Bradykinin (BK) has been proven to promote development and migration of head and neck squamous cell carcinoma (HNSCC) cells via epidermal development factor receptor (EGFR) transactivation. (biomasswars.com)
  • It is used for finding new ligands (enzyme inhibitors, receptor agonists and antagonists) to target proteins. (wikipedia.org)
  • regulate
  • In the cytoplasm, the encoded protein may function to regulate apoptosis. (wikipedia.org)
  • These complexes, which help regulate cell growth in addition to creating and maintaining epithelial layers, are known as adherens junctions and they typically include at least cadherin, β-catenin, and α-catenin. (wikipedia.org)
  • vitro
  • In this way, large libraries of proteins can be screened and amplified in a process called in vitro selection, which is analogous to natural selection. (wikipedia.org)
  • Phage display is also a widely used method for in vitro protein evolution (also called protein engineering). (wikipedia.org)
  • There is significant variation in the consequences of viroporin depletion: while hepatitis C virus is incapable of propagation without its p7 protein viroporin, influenza A virus and HIV-1 see decreases in in vitro viral titer of 10- to 100-fold in the absence of their respective viroporins, but remain capable of propagation. (wikipedia.org)
  • tumour
  • Senescent cells affect tumour suppression, wound healing and possibly embryonic/placental development and a pathological role in age-related diseases. (wikipedia.org)
  • respectively
  • The modification of isoCA-4 by introduction of difluoromethoxy group at the para-position (3i) and substitution of the two protons of the linker by two fluorine atoms (3m), produced the most active compounds in the series, with IC50 values of 0.15-2.2 nM (3i) and 0.1-2 nM (3m) respectively, against a panel of six cancer cell lines. (deepdyve.com)
  • transgenic
  • The experimental elimination of senescent cells from transgenic progeroid mice and non-progeroid, naturally-aged mice led to greater resistance against aging-associated diseases. (wikipedia.org)
  • Junctions
  • For instance, when an epithelial layer is complete and the adherens junctions indicate that the cell is surrounded, β-catenin may play a role in telling the cell to stop proliferating, as there is no room for more cells in the area. (wikipedia.org)
  • α-catenin participates in the formation and stabilization of adherens junctions by binding to β-catenin-cadherin complexes in the cell. (wikipedia.org)
  • spatial
  • To meet such complicated demands in biological process via spatial and temporal proteolysis, protein substrates have to be recognized, recruited, and eventually hydrolyzed in a well controlled fashion. (wikipedia.org)
  • fluorescence
  • Adjustments in the Ca2+ focus had been indicated as F/F, where F was the fluorescence strength when cells had been at rest, and F was the modification in fluorescence during excitement. (cancercurehere.com)
  • telomere length
  • May play also an essential role in telomere length maintenance and telomere capping in mammalian cells. (abcam.cn)
  • Cells can also be induced to senesce via DNA damage in response to elevated reactive oxygen species (ROS), activation of oncogenes and cell-cell fusion, independent of telomere length. (wikipedia.org)
  • HUMAN
  • Full length protein (Human). (abcam.cn)
  • The conundrum of the last decade or so, Wahlestedt said, is the fact that out of the three billion base pairs of DNA in the human genome, only slightly more than one percent of them actually produce proteins. (scripps.edu)
  • ab94065 is a 293T cell transfected lysate in which Human IKK beta has been transiently over-expressed using a pCMV-IKK beta plasmid. (abcam.com)
  • Her son, the Sage of the Six Paths, was born with the ability to produce chakra by combining physical energy (身体エネルギー, shintai enerugī), drawn from the trillions of cells that make up the human body, and mental energy (精神エネルギー, seishin enerugī), gained through experience and meditation. (wikipedia.org)
  • Human immunodeficiency virus type-1 accessory protein Vpr: a causative agent of the AIDS-related insulin resistance/lipodystrophy syndrome? (wikipedia.org)
  • binds
  • By immobilizing a relevant DNA or protein target(s) to the surface of a microtiter plate well, a phage that displays a protein that binds to one of those targets on its surface will remain while others are removed by washing. (wikipedia.org)
  • Expression
  • The study shows that a noncoding antisense form of RNA - an RNA that does not encode a protein- controls the expression of β-secretase-1 (BACE1), an enzyme critical to Alzheimer's disease progression. (scripps.edu)
  • Daxx is uniformly expressed throughout the body, except in the testes and thymus, which have especially high expression of the protein. (wikipedia.org)
  • No expression of Daxx leads to malfunction of S phase and cells with two nuclei are formed. (wikipedia.org)