• recombinant
  • We have previously demonstrated that the replacement of the S gene from an avirulent strain (Beaudette) of infectious bronchitis virus (IBV) with an S gene from a virulent strain (M41) resulted in a recombinant virus (BeauR-M41(S)) with the in vitro cell tropism of the virulent virus but that was still avirulent. (jove.com)
  • The recombinant IBV was in effect a chimaeric virus with the replicase gene derived from Beaudette and the rest of the genome from M41. (jove.com)
  • Recovery of a viable recombinant IBV also demonstrated that it is possible to interchange a complete replicase gene as we had in effect replaced the M41 replicase gene with the Beaudette derived gene. (jove.com)
  • antiviral
  • BACKGROUND: The rapid and accurate diagnosis of influenza facilitates antiviral therapy, judicious antibiotic usage, and cohorting patients to decrease nosocomial infection. (flu.org.cn)
  • antigens
  • It is known that physical linkage of TLR ligands and vaccine antigens significantly enhances the immunopotency of the linked antigens. (flu.org.cn)
  • diseases
  • The diminished T cell activation and cytokine production in the presence of allopurinol support a direct action of allopurinol on human T cells, offering a potential pharmacological tool for the management of cell-mediated inflammatory diseases. (frontiersin.org)
  • doses
  • In the Reuters Narcolepsy article, it states that GSK administered 30 million doses of the Pandermix vaccine and 800 developed narcolepsy, mostly children. (cellculturedish.com)
  • FluBlok
  • Developed by Protein Sciences Corporation, FluBlok is produced with insect cells. (wikipedia.org)
  • Made by Protein Sciences Corp. of Meriden, Connecticut, FluBlok would have been the first cell-line influenza vaccine licensed in the U.S. (6) Novartis must have been thrilled that its closest U.S. cell-line competitor was knocked out of the running days before it officially announced the launch of its Holly Springs plant. (huffingtonpost.com)
  • candidates
  • While there are several other Dengue Fever vaccines candidates in various phases of development, Sanofi's is the furthest along. (cellculturedish.com)
  • This led to early field trials in the 1930s of vaccine candidates developed using material taken from polio-infected monkeys, such as monkey spinal cords. (historyofvaccines.org)
  • 1992
  • An inactivated mouse brain--derived JE vaccine (JE-VAX [JE-MB]) has been licensed since 1992 to prevent JE in persons aged ≥1 year traveling to JE-endemic countries. (cdc.gov)
  • describes
  • A September 2012 New York Times article, In a Setback, Sanofi's Dengue Fever Vaccine Falls Short of Its Goal , describes the disappointing results in more detail. (cellculturedish.com)
  • This report summarizes the epidemiology of JE, describes the two JE vaccines that are licensed in the United States, and provides recommendations for their use among travelers and laboratory workers. (cdc.gov)
  • The ECACC describes itself as having one of the "premier collections of authenticated cell cultures in the world. (huffingtonpost.com)
  • Species
  • Allopurinol attenuated CD69 upregulation after CD3 and CD28 engagement and significantly reduced the levels of spontaneous and mitogen-induced intracellular reactive oxygen species in T cells. (frontiersin.org)
  • It holds more than 40,000 cell lines representing 45 different species and 50 different tissue types. (huffingtonpost.com)
  • The nectaries of the examined species differed in their width and height, number of layers and thickness of secretory parenchyma, and the height of epidermal cells. (worldwidescience.org)
  • laboratory
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • JE vaccine is recommended for travelers who plan to spend a month or longer in endemic areas during the JEV transmission season and for laboratory workers with a potential for exposure to infectious JEV. (cdc.gov)
  • fetal
  • The growth factors used to supplement media are often derived from the serum of animal blood, such as fetal bovine serum (FBS), bovine calf serum, equine serum, and porcine serum. (wikipedia.org)
  • tissues
  • Cells can be isolated from tissues for ex vivo culture in several ways. (wikipedia.org)
  • Cells can be isolated from solid tissues by digesting the extracellular matrix using enzymes such as collagenase, trypsin, or pronase, before agitating the tissue to release the cells into suspension. (wikipedia.org)
  • toxic
  • Although WHO cannot technically interfere with the internal laws of the US, they can and already have put enormous pressure on our government (and, thereby, our own laws) to require all citizens to get these toxic vaccine shots. (wordpress.com)
  • Use of the loop allows utilization of such substances at high concentrations while eliminating their cell toxic effects. (patentgenius.com)
  • Novartis
  • There is no concern about harming or killing anyone, because the companies (GlaxoSmithKline, Baxter, and Novartis, and others) have been indemnified by the government, so that there is absolutely no recourse for any deaths or injuries these vaccines may cause. (wordpress.com)
  • For its part in the deal, Novartis is required to provide two commercial-scale lots of "pre-pandemic" vaccine annually for a minimum of three years. (huffingtonpost.com)
  • production
  • In the current study, we have assessed the effect of allopurinol on antigen-specific and mitogen-driven activation and cytokine production in human T cells. (frontiersin.org)
  • Supplies of this vaccine are limited because production has ceased. (cdc.gov)
  • 1910
  • Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907 to 1910, establishing the methodology of tissue culture. (wikipedia.org)
  • safety
  • Please NOTE: As of August 1, 2009, no long-term, independent safety trial studies have been done for any of the different and new flu vaccines that are supposed to be given/forced on all Americans -whether we want them or not. (wordpress.com)