• kinetochore
  • During prometaphase, dynactin also helps target polo-like kinase 1 (Plk1) to kinetochores through cyclin dependent kinase 1 (Cdk1)-phosphorylated DCTN6/p27, which is involved in proper microtubule-kinetochore attachment and recruitment of spindle assembly checkpoint protein Mad1. (wikipedia.org)
  • vitro
  • In the branching nucleation described above, the addition of TPX2 to the egg extracts led to a dramatic increase in nucleation events-while in other studies, the protein XMAP215, in vitro, nucleated microtubule asters with its depletion in vivo reducing nucleation potential of centrosomes. (wikipedia.org)
  • filaments
  • Usually, it is the C-terminal domain of the MAP that interacts with tubulin, while the N-terminal domain can bind with cellular vesicles, intermediate filaments or other microtubules. (wikipedia.org)
  • The composing cytoskeletal elements of axoplasm, neural filaments, and microtubules provide the framework for axonal transport which allows for neurotransmitters to reach the synapse. (wikipedia.org)
  • In earlier work, Frey's group had shown that the density of molecular motors attached to the filaments has an impact on whether the microtubule grows or shrinks, and that their effect depends on the length of the filament concerned. (uni-muenchen.de)
  • cytokinesis
  • At cytokinesis, the last phase of the cell cycle, rapid dephosphorylation of stathmin occurs to block the cell from entering back into the cell cycle until it is ready. (wikipedia.org)
  • Without this regulation, signaling faults in cytokinesis can occur, resulting in unequal chromosome segregation or polyploid cells, greatly increasing the risk of cancer. (wikipedia.org)
  • regulates
  • Cystic fibrosis patients carry a defective gene that disables or destroys its protein product, which normally regulates the transport of ions across cell borders. (bio-medicine.org)
  • It regulates the movement of molecules into and out of the cell. (brainscape.com)
  • molecular
  • Methods in molecular biology (Clifton, N.J. (rochester.edu)
  • Molecular Biology and Evolution. (wikipedia.org)
  • Molecular Biology of the Cell. (wikipedia.org)
  • Molecular motors walking along a microtubule. (uni-muenchen.de)
  • Molecular Biology of the Cell , 2010 ;21:2661 -2673. (siue.edu)
  • Tolić completed her Diploma in Molecular Biology from the University of Zagreb, Croatia in 1996 and her Graduate Studies in Molecular Biology in the group of Prof. Nenad Trinajstić, Ruđer Bošković Institute and University of Zagreb, Croatia in 1999. (wikipedia.org)
  • Following her Post-doctoral training, Tolić was appointed as Research Group Leader (W1, equivalent to Assistant Professor) at the Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany in 2005 and was promoted to Senior Research Group Leader (W2, equivalent to Associate Professor) in 2010. (wikipedia.org)
  • Patrick J. Hussey is the Professor of Plant Molecular Cell Biology at the University of Durham. (dur.ac.uk)
  • Cell and developmental biologists at the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden devote their research to discovering how cell division and cell differentiation work, which structures can be found in cell organelles and how cells exchange information and materials. (mpg.de)
  • He recently was appointed as Director at the the Max Planck Institute for Molecular Cell Biology and Genetics, and the Klaus-Tschira Chair of the Systems Biology Center in Dresden. (mpg.de)
  • Kai Simons, founding director of the Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) and managing director of Lipotype GmbH, receives the Robert Koch Medal in Gold for his lifetime achievements, in particular for his characterization of membrane-forming lipids and the development of the lipid raft concept. (mpg.de)
  • Nature Structural & Molecular Biology. (wikipedia.org)
  • The Northwestern blot, also known as the Northwestern assay, is a hybrid analytical technique of the Western blot and the Northern blot, and is used in molecular biology to detect interactions between RNA and proteins. (wikipedia.org)
  • Molecular and Cellular Biology. (wikipedia.org)
  • lattice
  • The dynamic instability that makes it possible for microtubules to transition from a rigid polymerized or "assembled" nucleotide state to a flexible depolymerized or "disassembled" nucleotide state is driven by guanosine triphosphate (GTP) hydrolysis in the microtubule lattice. (lbl.gov)
  • Our study shows how EB proteins can either facilitate microtubule assembly by binding to sub-units of the microtubule, essentially holding them together, or else cause a microtubule to disassemble by promoting GTP hydrolysis that destabilizes the microtubule lattice," says Eva Nogales, a biophysicist with Berkeley Lab's Life Sciences Division who led this research. (lbl.gov)
  • intracellular
  • Dynactin or Dynein activator complex is a multi-subunit protein found in eukaryotic cells that aids in bidirectional intracellular transport by binding to dynein and kinesin-2 and linking them to the organelle or vesicle to be transported. (wikipedia.org)
  • mammalian
  • Mammalian Drf3 contains a CRIB-like motif within its GBD for binding to Cdc42, which is required for Cdc42 to activate and guide Drf3 towards the cell cortex where it remodels the actin skeleton. (wikipedia.org)
  • modulates
  • In this work, we show that the Ska3 C-terminal domain modulates the microtubule binding activity of the Ska complex by directly interacting with microtubules and Ska1 microtubule binding domain. (ed.ac.uk)
  • Dynactin is often essential for dynein activity and can be thought of as a "dynein receptor" that modulates binding of dynein to cell organelles which are to be transported along microtubules. (wikipedia.org)
  • novel
  • A better understanding of how microtubule dynamic instability is regulated could open new opportunities for improving the potency and selectivity of existing anti-cancer drugs, as well as facilitate the development of novel agents," Nogales says. (lbl.gov)