• necrosis
  • Here, we further characterized the antisense-transfected cell line, and the results showed that these cells formed small, minimally vascularized tumors exhibiting extensive necrosis. (aacrjournals.org)
  • However, under the severe levels of oxidative stress that cause necrosis, the damage causes ATP depletion, preventing controlled apoptotic death and causing the cell to simply fall apart. (wikipedia.org)
  • genes
  • In individual cells, the circadian clock mechanism consists of oscillating feedback loops of transcription of "core" oscillator genes and posttranslational modifications of their protein products that regulate protein stability, activity, and/or localization. (pnas.org)
  • Uncontrolled cell proliferation is the hallmark of cancer, and cancer cells have typically acquired damage to genes that directly regulate their cell cycles. (springer.com)
  • Increased copy number of TERT and TERC telomerase subunit genes in cancer cells. (springer.com)
  • In the present study, we investigated the effect of CS on the expression of progesterone production-related genes in KGN cells, derived from human granulosa-like tumor. (nii.ac.jp)
  • Analysis of B02 cells transcriptional profile revealed that 11 and 9 out of the 50 most up- and down-regulated mRNAs, respectively, corresponded to genes which expression has been previously associated with osteoblastic differentiation process. (springer.com)
  • cancer
  • Located at 18q21, LINC-ROR (regulator of reprogramming) is a modulator of ESCs maintenance and hypoxia-signaling pathways in hepatocellular cancer cells. (springer.com)
  • The aim of this study was to examine the expression of LINC-ROR in various cell lines and representative samples of human cancers by quantitative real-time RT-PCR to provide a snapshot on how LINC-ROR expression may be deregulated in cancer. (springer.com)
  • Our results revealed that the expression of LINC-ROR was lower in all somatic cancer cell lines compared to stem cells or cells with stem cell-like capabilities, like the embryonic carcinoma cell line, NTERA-2. (springer.com)
  • Thus, we hypothesize that LINC-ROR may have a key function in a subpopulation of cells from the tumor bulk, i.e., the cancer stem cells associated with specific properties including resistance to adverse environmental conditions. (springer.com)
  • Functional characterisation of osteosarcoma cell lines and identification of mRNAs and mirnas associated with aggressive cancer phenotypes. (springer.com)
  • Trebunova M, Laputkova G, Slaba E, Lacjakova K, Verebova A. Effects of docetaxel, doxorubicin and cyclophosphamide on human breast cancer cell line mcf-7. (springer.com)
  • Certain p53 mutants may enhance drug resistance in cancer cells. (springer.com)
  • To determine whether two frequently occurring p53 mutants, R248Q and R273C, would increase the drug resistance of liver cancer cells, stable cell lines expressing these specific p53 mutants were established by transfecting the p53 -null Hep3B cells with mutant p53 expression vectors, and then treating them with the anticancer drugs doxorubicin and paclitaxel. (springer.com)
  • These results suggest that expression of the p53 mutant, R248Q, in liver cancer cells may enhance their drug resistance and that upregulation of P-glycoprotein activity may contribute to this protective effect. (springer.com)
  • Hemaiswarya S, Doble M. Combination of phenylpropanoids with 5-fluorouracil as anti-cancer agents against human cervical cancer (HeLa) cell line. (springer.com)
  • Topotecan- or mitoxantrone-selected cell lines (T8 and MX3, respectively), derived from the human IGROV1 ovarian cancer cell line, were resistant to the topoisomerase I inhibitors topotecan, SN-38 (the active metabolite of irinotecan), and 9-aminocamptothecin, as well as to the topoisomerase II drug mitoxantrone. (aacrjournals.org)
  • Cells selected with TPT or MX from the human IGROV1 ovarian cancer cell line were resistant to TPT, as well as to the analogues SN-38 and 9-AC, with concomitant overexpression of BCRP/MXR/ABCP . (aacrjournals.org)
  • Telomerase, a ribonucleoprotein enzyme is considered as a universal therapeutic target of cancer because of its preferential expression in cancer cells and its presence in 90 % of tumors. (springer.com)
  • Specific association of human telomerase activity with immortal cells and cancer. (springer.com)
  • Inhibition of telomerase activity in endometrial cancer cells by selenium-cisplatin conjugate despite suppression of DNA-damaging activity by sodium ascorbate. (springer.com)
  • Telomerase activity is associated with cell cycle deregulation in human breast cancer. (springer.com)
  • Heparanase facilitates the invasion and metastasis of cancer cells, and is over-expressed in many kinds of malignancies. (springer.com)
  • The aim of this study is to determine whether silencing of heparanase expression can abolish the malignant characteristics of gastric cancer cells. (springer.com)
  • Three heparanase-specific small interfering RNA (siRNAs) were designed, synthesized, and transfected into cultured gastric cancer cell line SGC-7901. (springer.com)
  • The in vitro invasion and metastasis of cancer cells were measured by cell adhesion assay, scratch assay and matrigel invasion assay. (springer.com)
  • The angiogenesis capabilities of cancer cells were measured by tube formation of endothelial cells. (springer.com)
  • In addition, the in vitro invasion and metastasis of cancer cells were attenuated after knock-down of heparanase. (springer.com)
  • Moreover, transfection of heparanase-specific siRNA attenuated the in vitro angiogenesis of cancer cells in a dose-dependent manner. (springer.com)
  • These studies suggest that HPA is correlative with invasion and metastasis of cancer cells, and served as an important target of cancer therapy. (springer.com)
  • One of the most remarkable advances in our understanding of cancer pathogenesis is that the progression of solid tumors depends on tumor angiogenesis. (aacrjournals.org)
  • Cancer Cell 11:291-302. (springer.com)
  • Cancer Cell 3:565-576. (springer.com)
  • Metastatic breast cancer cells exhibit the selective ability to seed and grow in the skeleton. (springer.com)
  • In an attempt to reveal the osteoblast-like phenotype of osteotropic breast cancer cells, we performed a microarray study on a model of breast cancer bone metastasis consisting of the MDA-MB-231 human cell line and its variant B02 selected for its high capacity to form bone metastases in vivo. (springer.com)
  • Bellahcène A, Kroll M, Liebens F, Castronovo V (1996) Bone sialoprotein detection in human breast cancer cells: a potential predictor of bone metastases. (springer.com)
  • Today CD133 is the most commonly used marker for isolation of cancer stem cell (CSC) population from different tumors, mainly from various gliomas and carcinomas. (wikipedia.org)
  • Moreover, there are two antigenic epitopes (CypB84-92 and CypB91-99) recognized by HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes which could be used as cancer vaccines, and in fact, were used to treat lung cancer in a clinical trial. (wikipedia.org)
  • The presence of serum spreading factor was shown to influence directed migration along a gradient of substrate molecules in a few types of cancer cells. (wikipedia.org)
  • Combined small cell lung carcinoma (or c-SCLC, and rarely rendered as "small-cell lung carcinoma") is a form of multiphasic lung cancer that is diagnosed by a pathologist when a malignant tumor arising from transformed cells originating in lung tissue contains a component of small cell lung carcinoma (SCLC) admixed with one (or more) components of non-small cell lung carcinoma (NSCLC). (wikipedia.org)
  • However, since different forms of malignant tumors generally exhibit diverse genetic, biological, and clinical properties - including response to treatment - accurate classification of lung cancer cases are critical to assuring that patients with lung cancer receive optimum management. (wikipedia.org)
  • Under WHO-2004, lung carcinomas are divided into 8 major taxa: Squamous cell carcinoma Small cell carcinoma Adenocarcinoma Large cell carcinoma Adenosquamous carcinoma Sarcomatoid carcinoma Carcinoid tumor Salivary gland-like carcinoma SCLC is generally considered to be the most aggressive of these major forms of lung cancer, with the worst long term prognosis and survival rates. (wikipedia.org)
  • In the same report, these authors also reported the exquisite anticancer activity of halichondrin B against murine cancer cells both in culture and in in vivo studies. (wikipedia.org)
  • However, in ER-positive breast cancer cell line MCF-7, estradiol caused a dose-dependent decrease in LKB1 transcript and protein expression leading to a significant decrease in the phosphorylation of the LKB1 target AMPK. (wikipedia.org)
  • vivo
  • This cell line exhibits markedly reduced rate of tumor growth in vivo . (aacrjournals.org)
  • In one experiment, the addition of PPIB into cell cultures in vitro induced chemotaxis and integrin-mediated adhesion of T cells to the extracellular matrix (ECM), suggesting that it might function in innate immunity by recruing T cells into infected tissue in vivo. (wikipedia.org)
  • human
  • Endogenous miRNA sponge lincRNA-RoR regulates Oct4, Nanog, and Sox2 in human embryonic stem cell self-renewal. (springer.com)
  • Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells. (springer.com)
  • Human osteosarcoma cell line U2OS was used for the experiments. (springer.com)
  • In this report, we demonstrate for the first time that overexpression of BCRP/MXR/ABCP in human tumor cells cannot only be invoked by exposure to MX but also by exposure to the clinically important drug TPT. (aacrjournals.org)
  • Publications] Fukawa E et al: 'Homologous down-regulation of the glucourticoid receptor down-modulates cellular hormone responsiveness in human histiocytic Cymphome U93t cells. (nii.ac.jp)
  • Homologous down-regulation of the glucocorticoid receptor down-modulates cellular hormone responsiveness in human histiocytic lymphoma U937 cells. (nii.ac.jp)
  • The vast majority of human pancreatic cancers (∼95%) are classified as ductal adenocarcinomas ( 1, 2 ), whereas acinar cell carcinomas and other histologic types are much less common. (springer.com)
  • Some studies have suggested that it arises from metaplasia (transdifferentiation) of acinar cells or even endocrine (islet) cells to ductal cells leading to ductal adenocarcinoma ( 3, 4 ) Although this has been based on cell lines and animal models, observations on human carcinomas, however, imply a different scenario. (springer.com)
  • This chapter provides an overview of the histologic and molecular complexities of pancreatic neoplasia as they apply to and correlate with different animal models, with a focus on transgenic mice and mice bearing human pancreatic tumor cells as xenografts, and their potential utility for developing preventive and/or therapeutic strategies. (springer.com)
  • We and others have previously reported that human breast tumors which metastasize to the skeleton overexpress bone matrix extracellular proteins. (springer.com)
  • These results were based on a mouse model and although they are mammalian cells, it has not been shown to apply to human cells. (wikipedia.org)
  • proteins
  • Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. (abgent.com)
  • The E6 and E7 viral oncoproteins are the primary proteins responsible for cell homeostasis alteration and immortalization. (springer.com)
  • In cardiac myogenic cells, cyclophilins have been observed to be activated by heat shock and hypoxia-reoxygenation as well as complex with heat shock proteins. (wikipedia.org)
  • Disturbances in the normal redox state of cells can cause toxic effects through the production of peroxides and free radicals that damage all components of the cell, including proteins, lipids, and DNA. (wikipedia.org)
  • tissues
  • Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications. (abgent.com)
  • More than 30 cell lines and 112 patient specimens from various tissues were assessed for relative expression of LINC-ROR . (springer.com)
  • nude
  • Therefore, our results demonstrate that reduced tumor growth in nude mice by suppression of GD3-synthase expression in F-11 cells results from minimal angiogenesis of the tumors through down-regulation of the VEGF expression, which indicates an important role for GD3 in tumor angiogenesis. (aacrjournals.org)
  • novel
  • We introduce a novel in vitro phenomenon of osteosarcoma (OS) cell line caused by low-dose DOX-induced oxidative stress. (springer.com)
  • carcinoma
  • The epidemiology of renal cell carcinoma. (springer.com)
  • Contemporary clinical epidemiology of renal cell carcinoma: insight from a population based case-control study. (springer.com)
  • Age-adjusted incidence, mortality, and survival rates of stage-specific renal cell carcinoma in North America: a trend analysis. (springer.com)
  • Surveillance after radical or partial nephrectomy for localized renal cell carcinoma and management of recurrent disease. (springer.com)
  • Currently, the only exception to this recommendation occurs in cases where anaplastic large cell lung carcinoma (LCLC) is the second histological component. (wikipedia.org)
  • In a study of 408 consecutive patients with SCLC, Quoix and colleagues found that presentation as a solitary pulmonary nodule (SPN) is particularly indicative of a c-SCLC - about 2/3 of their SPN's were pathologically confirmed to be c-SCLC's containing a large cell carcinoma component. (wikipedia.org)
  • suppression
  • This enzyme has also been identified as PLA2G16, HRASLS3, HREV107, HREV107-3, MGC118754 or H-REV107-1 from studies on class II tumor suppression but not on its enzymatic properties. (wikipedia.org)
  • polarity
  • Activation of AMPK-related kinases by LKB1 plays vital roles maintaining cell polarity thereby inhibiting inappropriate expansion of tumour cells. (wikipedia.org)
  • A picture from current research is emerging that loss of LKB1 leads to disorganization of cell polarity and facilitates tumour growth under energetically unfavorable conditions. (wikipedia.org)
  • xenografts
  • Significantly fewer new vessels were found on the antisense xenografts and the skin around them than those on/around the xenografts formed by the sense-transfected and untransfected F-11 cells. (aacrjournals.org)
  • The hemoglobin content of the antisense xenografts was much lower than that of the xenografts formed by the control cells. (aacrjournals.org)
  • The expression of VEGF was suppressed in the antisense xenografts and the conditioned culture media of the antisense-transfected F-11 cells as determined by Western blotting analysis. (aacrjournals.org)
  • plasma membrane
  • Yusa K, Tsurio T (1989) Reversal mechanism of multidrug resistance by verapamil: direct binding of verapamil to P-glycoprotein on specific sites and transport of verapamil outward across the plasma membrane of K562/ADM cells. (springer.com)
  • pathway
  • As a cyclophilin, PPIB binds the immunosuppressive drug CsA to form a CsA-cyclophilin complex, which then targets calcineurin to inhibit the signaling pathway for T-cell activation. (wikipedia.org)
  • overexpression
  • The aim of this study was to evaluate the effect of HTRA1 overexpression in HPV16-positive (CasKi) and HPV-negative (C33) cervical cell lines. (springer.com)
  • Overexpression of BCRP/MXR/ABCP , caused by exposure of the cells to MX or doxorubicin/verapamil, resulted in a resistance pattern that was different from that generally observed in the cases of P-gp or MRP1 overexpression. (aacrjournals.org)
  • induces
  • TGF-β1 is a cytokine found in higher concentrations of lungs from patients who have IPF, and induces haptotaxis of pleural mesothelial cells. (wikipedia.org)
  • tissue
  • It is also associated with viral infections.In eukaryotes, cyclophilins localize ubiquitously to many cell and tissue types. (wikipedia.org)
  • Mechanical effects like the buildup of tensile forces may play an important role for both division as well as motility of cells in tissue. (wikipedia.org)
  • progression
  • A cell cycle immunofluorescence assay revealed more CasKi cells overexpressing HTRA1 in the S phase and more C33 HTRA1 -transfected cells in the G0/G1 phase, suggesting that HTRA1 plays different roles in the cell cycle progression of these cells. (springer.com)
  • Ganglioside GD3 is overexpressed in many types of tumors and may be associated with tumor progression and the development of metastatic potential. (aacrjournals.org)
  • expression
  • However, when the cells were pre-treated with NAC, no DOX- or H 2 O 2 -mediated increase of invasiveness or MMP-9 expression was evident. (springer.com)
  • Loss of either NONO or PER abolished this activation and circadian expression of p16-Ink4A and eliminated circadian cell cycle gating. (pnas.org)
  • Furthermore, BCRP/MXR/ABCP expression levels in various partially revertant T8 cells correlated with the levels of resistance to topotecan, SN-38, and mitoxantrone, strongly suggesting BCRP/MXR/ABCP to be the transporter responsible for the enhanced efflux. (aacrjournals.org)
  • Furthermore, BCRP/MXR/ABCP expression levels correlated with the levels of resistance in various partially revertant cell lines. (aacrjournals.org)
  • It is important to note that this increased expression was not found on the capillary endothelial cells. (wikipedia.org)
  • differentiation
  • 4 In addition to its ability to reduce vascular tone, it stimulates hormone secretion 5 and cell growth and differentiation. (ahajournals.org)
  • Telomerase activity is downregulated via decreases in hTERT mRNA but not TEP1 mRNA or hTERT during the differentiation of leukemic cells. (springer.com)
  • The evidence thus suggests that ductal adenocarcinomas may simply be originating from ductal cells, although it cannot be ruled out that ductal metaplasia of other cell types, especially acinar cells or centro-acinar cells (trans-differentiation or formation of ductular structures) could also be involved in the development of ductal adenocarcinoma under different situations, including genetic or epigenetic processes. (springer.com)
  • Thus, osteoblast specific cadherin 11 which mediates the differentiation of mesenchymal cells into osteoblastic cells is up-regulated in B02. (springer.com)
  • oxidative stress
  • The results suggest that oxidative stress induced by low-dose DOX promotes the invasiveness of osteosarcoma cell line U2OS in vitro, through MMP-9 induction. (springer.com)
  • The effects of oxidative stress depend upon the size of these changes, with a cell being able to overcome small perturbations and regain its original state. (wikipedia.org)
  • vitro
  • in vitro , 70% of the intracellular topotecan pool was transported out of the T8 or MX3 cells within 30 s. (aacrjournals.org)
  • membrane
  • Cholesterol sulfate (CS) is a component of cell membranes that plays a role in stabilizing the cell membrane. (nii.ac.jp)
  • While the precise function of CD133 remains unknown, it has been proposed to act as an organizer of cell membrane topology. (wikipedia.org)