Cell mrnas polyadenylation. Medical search. Frequent questions

Cytoplasmic polyadenylation element-binding protein 2 (CPEB2) is a sequence-specific RNA-binding protein and translational regulator. (tmu.edu.tw)

Beyond this, the 5' cap has also been shown to have a role in exporting the mature mRNA from the nucleus and into the cytoplasm. (wikipedia.org)
This is known as a Poly-A tail and is used for stability and guidance, so that the mRNA can exit the nucleus and find the ribosome. (wikipedia.org)
In a eukaryote, an organism with a nucleus in each of its cells, the DNA is stored inside the nucleus, so those instructions have to be transmitted to the cell by first making a copy of them in a polymer called messenger RNA or mRNA. (sciencing.com)
mRNA is edited by cellular machinery before it leaves the nucleus, and several important molecular features are added to it to mark it as finished and ready for use. (sciencing.com)
This tail appears to make the mRNA more stable and marks it as destined for export from the nucleus. (sciencing.com)
Eukaryotic mRNA is sometimes edited or spliced before it leaves the nucleus, so they need to regulate which mRNAs can leave the nucleus. (sciencing.com)
Unlike eukaryotes, prokaryotes have no nucleus so there's no need to regulate entry or exit of mRNAs -- as soon as the mRNA is transcribed it's set loose in the cell. (sciencing.com)
Prior to active export factor-dependent transport from the nucleus to the cytosol, the newly formed pre-mRNA must mature. (uni-frankfurt.de)
Nearly all mRNAs in the cell are polyadenylated in the nucleus right after being transcribed from DNA and before the transport to the cytoplasm. (edu.pl)
Defects in mRNA 3'end formation have been described to alter transcription termination, transport of the mRNA from the nucleus to the cytoplasm, stability of the mRNA and translation efficiency. (nih.gov)
In addition to stabilizing the 5' end of mRNAs, they serve a diverse array of functions such as enhancing mRNA transport out of the CELL NUCLEUS and regulating MRNA TRANSLATION in the CYTOPLASM. (bvsalud.org)

Here, we report that mouse genes tend to express mRNAs with longer 3′ UTRs as embryonic development progresses. (northwestern.edu)
For example, neurons tend to express mRNAs with long 3'UTRs due to preferential processing at PASs more distal than other PASs used in other cell types. (stanford.edu)

The 5' cap is used to increase mRNA stability. (wikipedia.org)
Fertilization also leads to changes in mRNA translation and protein stability, which support a period of development driven off of maternal stockpiles. (elifesciences.org)
The 3' regulatory regions have a great diversity of cis -regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. (frontiersin.org)
Polyadenylation is essential for the stability of the transcript, preventing the mRNA from being the target of posttranscriptional gene silencing (PTGS) via RNA-dependent RNA polymerase 6 (RDR6) in plants ( Luo and Chen, 2007 ). (frontiersin.org)
Specifically, we are interested in post-translational modifications, changes to a protein after it's produced, which add an incredible level of diversity to the underlying proteins within a cell and can control a diverse array of processes, including protein stability, interactions, and activity. (upenn.edu)
2021) Cdc4 phospho-degrons allow differential regulation of Ame1CENP-U protein stability across the cell cycle. (oeaw.ac.at)
MOTIVATION: Pre-mRNA cleavage and polyadenylation are essential steps for 3'-end maturation and subsequent stability and degradation of mRNAs. (mdc-berlin.de)
APA can potentially affect mRNA translation efficiency, localization, stability, and mRNA seeded protein-protein interactions. (stanford.edu)
The ends of mRNA molecules are specifically modified in order to enhance their stability and ability to serve as a template for proteins synthesis at ribosomes: at the beginning, they contain so-called cap structure, while at the end, there is a poly(A) tail. (edu.pl)
Stress-adaptive cell plasticity in target tissues and cells for plant biomass growth is important for yield stability. (frontiersin.org)
microRNAs are small non-coding RNA fragments that bind to target mRNAs to regulate their stability and/or translation. (metu.edu.tr)
We will focus our research on the identification of its interacting partners and mRNA targets to understand how it regulates mRNA stability, polyadenylation and translation. (sorbonne-universite.fr)

A complex interaction between the cleavage and polyadenylation molecular complex and cis -elements determine the polyadenylation site, which may result in the choice of non-canonical sites, resulting in alternative polyadenylation events, involved in the regulation of more than 80% of the genes expressed in plants. (frontiersin.org)
2021) Principles of mRNA targeting and regulation via the Arabidopsis m6A-binding proteins ECT2 and ECT3. (oeaw.ac.at)
The 3′ untranslated regions (3' UTRs) of mRNAs contain c/s-acting elements for posttranscriptional regulation of gene expression. (northwestern.edu)
This global regulation is controlled by alternative polyadenylation and coordinates with initiation of organogenesis and aspects of embryonic development, including morphogenesis, differentiation, and proliferation. (northwestern.edu)
A growing body of evidence shows that lncRNAs play important functional roles in diverse biological processes, such as epigenetic regulation, cell cycle regulation, cellular growth and differentiation, by regulating the level of target genes [ 3 , 4 ]. (biomedcentral.com)
During meiosis, germ cell and stage-specific components impose additional layers of regulation on the core cell cycle machinery to set up an extended G2 period termed meiotic prophase. (stanford.edu)
Regulation and function of alternative polyadenylation in development and differentiation. (stanford.edu)
Recent studies revealed the presence of different mRNA isoforms, with distinct biological regulation. (biomed.news)
In this context, the functions of the individual cis and trans elements have been extensively studied, yet the regulation of alternative polyadenylation-the decision whether to use the proximal or distal PAS-is less deciphered and requires additional study. (uni-frankfurt.de)
In neurons, cytoplasmic polyadenylation of synaptic mRNAs plays a significant role in the regulation of protein synthesis. (edu.pl)
11 Both proteins possess an N-terminal RING finger motif and two BRCA1 C-terminal (BRCT) domains present in numerous proteins involved in DNA repair and cell cycle regulation. (bmj.com)
cancer cell proliferation gene expression mRNA biogenesis polyadenylation post-transcriptional regulation of gene expression. (silverchair.com)
Given the significance of microRNA-mediated gene expression regulation, we are interested in understanding how microRNA and mRNA interactions take place and how these interactions may be overridden by regulated alterations of 3' UTR sizes. (metu.edu.tr)
We focus our research on the post-transcriptional regulation of embryonic myogenesis and adult muscle differentiation by RNA-binding proteins.We use zebrafish as a model, which is most suitable for in vivo and in vitro analyses using cell and molecular biology, genetics, and living imaging approaches. (sorbonne-universite.fr)
We identified that CPEB2 promoted PDGFRα mRNA translation in MYF progenitors and this positive regulation could be disrupted by H 2 O 2 , a hyperoxia-mimetic treatment. (tmu.edu.tw)
Accumulating evidence highlights emerging functions of RBPs in the post-transcriptional regulation of inner ear development and hair cell function. (encyclopedia.pub)

CPEB-1 is an RNA-binding protein that interacts with the cytoplasmic polyadenylation element (CPE), a short U-rich motif in the 3' untranslated regions (UTRs) of certain mRNAs. (nih.gov)
The targeting event displaced the introns and coding regions and replaced the endogenous 3' UTRs (which contribute to mRNA destabilization and to Arc mRNA dendritic trafficking) with an exogenous SV40 polyadenylation signal (to promote high-level expression). (jax.org)
Alternative polyadenylation (APA) generates transcript isoforms that differ in the position of the 3' cleavage site, resulting in the production of mRNA isoforms with different length 3' UTRs. (stanford.edu)
To begin understanding this complex question, we are interested mainly in the 3' UTRs (untranslated regions) of mRNAs. (metu.edu.tr)

It functions as a translational regulator that plays a major role in the control of maternal CPE-containing mRNA in oocytes, as well as of subsynaptic CPE-containing mRNA in neurons. (nih.gov)
Project description: Neurons communicate with each other through synapses, specialized contact sites that enable electrical impulses to be transmitted between cells. (edu.pl)

High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis. (nature.com)

CPEB-3 is a sequence-specific translational regulatory protein that regulates translation in a polyadenylation-independent manner. (nih.gov)
We recently discovered a new type of stress-responsive, protein-directed human RNA switch that regulates expression of vascular endothelial growth factor-A in human monocytic cells (Ray, PS, et al. (rochester.edu)
We also identified a novel mRNA processing mechanism that expands human proteome at the posttranscriptional level and regulates gene expression (Yao, P, et al. (rochester.edu)
In the mammalian brain, alternative pre-mRNA splicing is a fundamental mechanism that modifies neuronal function dynamically where secretion of different splice variants regulates neurogenesis, development, pathfinding, maintenance, migration, and synaptogenesis. (aging-us.com)
This grant proposal describes how we wish to understand this metabolism of mRNA better by studying a protein (called Dcs2) that regulates a component step in the metabolism of RNA. (ukri.org)
Thus, our objective is to understand how RBM24 regulates protein homeostasis during muscle cell differentiation and regeneration. (sorbonne-universite.fr)

At the level of isoform characterization, we discovered widespread differences in the use of tandem alternative polyadenylation (APA) sites by methylated and nonmethylated transcript isoforms of individual genes. (nature.com)
Eukaryotes have a mechanism termed alternative polyadenylation (APA), which allows the selective use of PAS in genes containing multiple PAS. (frontiersin.org)
APA naturally occurs during development and cellular differentiation, with around 70% of human genes displaying APA in particular tissues and cell types. (stanford.edu)
We identified >500 Drosophila genes that express mRNA isoforms with a long 3' UTR in proliferating spermatogonia but a short 3' UTR in differentiating spermatocytes due to APA. (stanford.edu)
About 70% of mammalian genes have more than one polyadenylation site (PAS) and express transcripts of different lengths by a mechanism called alternative polyadenylation (APA). (uni-frankfurt.de)
Initial determination of the poly(A)-tome in P19 cells by MACE-Seq yielded approximately 16,000 PAS and showed that slightly less than 50% of all genes used two or more PAS and expressed alternative 3'UTR isoforms. (uni-frankfurt.de)
Here, we demonstrate that targeting either the mRNA polyadenylation signal and/or cleavage site is an efficient strategy to down-regulate DUX4 expression and to decrease the abnormally high-pathological expression of genes downstream of DUX4. (nih.gov)
MicroRNAs (miRNAs) negatively regulate the expression of the target genes by binding to ʻseed sequences' in the 3'‑untranslated region (3'‑UTR) mRNA transcripts, and the variants within or nearby ʻseed sequences' may compromise or enhance miRNA/mRNA interaction leading to either ʻloss‑of‑function' or ʻgain‑of‑function' effects. (spandidos-publications.com)
The information contained in the genes of living cells has to be converted into cellular components that form structures and enable biochemical reactions to take place. (ukri.org)
In transcription, the information in the DNA sequences of the genes is converted into equivalent sequences in so-called messenger RNA (mRNA) molecules. (ukri.org)
It is possible that Lin28B directly promotes mRNA translation of mTOR pathway genes or relieves let7 -mediated repression of their translation [ 4 ] . (encyclopedia.pub)

Specifically, alternative cleavage and polyadenylation (APA) is a co-transcriptional molecular process that switches the polyadenylation site (PAS) at which a nascent mRNA is cleaved, resulting in mRNA isoforms with different 3'UTR length and content. (stanford.edu)
In addition, changes in APA mark a variety of pathological states, including many types of cancer, in which mRNAs are preferentially cleaved at more proximal PASs, causing expression of mRNA isoforms with short 3'UTRs. (stanford.edu)
Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)
Here, we present a protocol for the absolute quantification of mRNA isoforms using microfluidic digital PCR (mdPCR). (biomed.news)
Following exonuclease I digestion to remove remaining single-stranded DNA, the samples are loaded onto a mdPCR chip with TaqMan probes targeting the mRNA isoforms of interest, whereupon target molecules are amplified in nanoliter chambers. (biomed.news)
We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)
This protocol enables the absolute quantification of low abundant mRNA isoforms in a fast, precise, and reliable way. (biomed.news)

LncRNAs are involved in a wide range of regulatory mechanisms that impact gene expression, including chromatin remodeling, modulation of alternative splicing, fine-tuning of miRNA activity, and the control of mRNA translation or accumulation [ 5 ]. (biomedcentral.com)

miRNAs bind to complementary sequences present in the 3′-untranslated regions (3′-UTR) of target gene mRNAs and, thus, regulate translational interference or degradation of mRNAs ( 8 ). (spandidos-publications.com)

Crystal structure of murine CsfF-77: Dimeric Association and implications for polyadenylation of mRNA precursors. (saborizate.com)

Lastly, in vivo C/P assays demonstrate a competition between fast, high affinity RNA binding and efficient, correct C/P. These results highlight the importance of the surface charge of the RRM in RNA binding and the balance between nascent mRNA binding and C/P in vivo. (ttu.edu)

The first chemical modification which all eukaryotic mRNAs share is called a 5' cap. (sciencing.com)
5' caps and poly-A tails are found in all eukaryotic mRNAs. (sciencing.com)
Moreover, polyadenylation is a crucial step in the maturation of almost all eukaryotic mRNAs, and thus all mRNAs are virtually eligible for this antisense-mediated knockdown strategy. (nih.gov)

CPEB-4 is an RNA-binding protein that mediates meiotic mRNA cytoplasmic polyadenylation and translation. (nih.gov)

Mature messenger RNA, often abbreviated as mature mRNA is a eukaryotic RNA transcript that has been spliced and processed and is ready for translation in the course of protein synthesis. (wikipedia.org)
Unlike the eukaryotic RNA immediately after transcription known as precursor messenger RNA, mature mRNA consists exclusively of exons and has all introns removed. (wikipedia.org)
The production of a mature mRNA molecule occurs in 3 steps: Capping of the 5' end Polyadenylation of the 3' end RNA Splicing of the introns During capping, a 7-methylguanosine residue is attached to the 5'-terminal end of the primary transcripts.This is otherwise known as the GTP or 5' cap. (wikipedia.org)
As a part of the maturation process, RNA splicing removes the non-coding RNA introns leaving behind the exons, which are then spliced and joined together to form the mature mRNA. (wikipedia.org)
In murine P19 cells, we were able to demonstrate for the first time a direct link between 3'UTR-APA and nuclear export of mature mRNA by the splicing factors SRSF3 and SRSF7 and decipher the mechanism. (uni-frankfurt.de)

This subfamily corresponds to the RRM1 of the CPEB family of proteins that bind to defined groups of mRNAs and act as either translational repressors or activators to regulate their translation. (nih.gov)
It preferentially binds to poly(U) RNA oligomers and may regulate the translation of stored mRNAs during spermiogenesis. (nih.gov)
Cell-type-specific interacting proteins collaborate to regulate the timing of Cyclin B protein expression in male meiotic prophase. (stanford.edu)
The precise mechanism by which Lin28B and let7 miRNA regulate mTORC1 activity in cochlear epithelial cells awaits further investigation. (encyclopedia.pub)

Histological staining and immunohistochemistry were used to analyze morphology, proliferation, apoptosis and cell densities from postnatal to adult lungs. (tmu.edu.tw)
Western blotting, RNA-immunoprecipitation, reporter assay, primary MYF culture and ectopic expression rescue were performed to demonstrate the role of CPEB2 in PDGFRα mRNA translation and MYF proliferation. (tmu.edu.tw)
In neonatal murine cochlear organoids and explants, Lin28B antagonizes the activity of let7 miRNA and increases Akt-mTORC1 signaling to promote hair cell regeneration from immature supporting cells by inducing their de-differentiation and proliferation as well as by directly converting them into hair cells [ 3 ] . (encyclopedia.pub)

When there are multiple polyadenylation sites on the same mRNA molecule, alternative polyadenylation can occur. (wikipedia.org)
Sensitive ADAR editing reporter in cancer cells enables high-throughput screening of small molecule libraries. (cancerindex.org)
In a single-well setup we used the reporter in HeLa cells to screen a small molecule library of 33 000 compounds. (cancerindex.org)
More specifically, cells of animals (including man), plants, insects and fungi modify the beginning (called the 5'end) of each mRNA molecule by adding a methylated guanosine base in a structure called the cap. (ukri.org)

abstract = "Nascent pre-mRNA 3′-end cleavage and polyadenylation (C/P) involves numerous proteins that recognize multiple RNA elements. (ttu.edu)

Dynamic m 6 A mRNA methylation directs translational control of heat shock response. (nature.com)

Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. (cancerindex.org)
In translation, the mRNA molecules are 'read' by a large molecular structure called the ribosome, which uses the information to dictate the synthesis of proteins. (ukri.org)
Each cell has to synthesize and break down its mRNA molecules, because this continuous turnover allows the cell to adapt its patterns of gene expression more rapidly to changes in the demand for particular proteins which are needed under certain growth conditions. (ukri.org)

The power of the thiol proteinases to cleave a multitude of substrates in response to calcium mineral activation allows their involvement in a variety of cell processes offering cell motility, sign transduction, apoptosis, cell differentiation and legislation of the cytoskeleton (3). (exposed-skin-care.net)
Cell differentiation is a fundamental process that generates all the cell types of our body from a fertilized egg. (sorbonne-universite.fr)
In the cochlea of mouse embryos, it is highly expressed in prosensory cells and down-regulated at the onset of hair cell differentiation. (encyclopedia.pub)
Prolonged expression of Lin28B delays prosensory cell cycle exit and prevents hair cell differentiation, suggesting that it functions to increase hair cell production [ 2 ] . (encyclopedia.pub)
Therefore, the antagonistic actions of Lin28B and let7 miRNA coordinate the timing of prosensory cell cycle withdrawal for hair cell differentiation. (encyclopedia.pub)
Thus, Lin28B functions in hair cell regeneration through mitotic and non-mitotic mechanisms, which are dependent on mitotic division or trans-differentiation of supporting cells into hair cells, respectively. (encyclopedia.pub)

The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the trichodiene synthases. (justia.com)
However, intergenic regions between all major ORFs contained relatively conserved motifs that could be transcription termination/polyadenylation sequences (A/U) CU 7 , similar to other rhabdoviruses ( 5 ). (cdc.gov)

Using advanced NGS techniques (such as Hi-C, HiChIP, RNA-seq, ChIP-seq, ATAC-seq etc) and a variety of CRISPR-based technologies I dissected the interplay between transcription factors, histone modifications and chromatin conformation changes during somatic cell reprogramming from mouse embryonic fibroblasts to induced pluripotent cells, and integrated this data with transcriptional changes at different stages of reprogramming. (iit.it)

This process of removing the cap structure ('decapping') affects the levels of RNA decay intermediates in the cell that are capable of inhibiting important cellular processes. (ukri.org)
The m7G cap at the 5'end of mRNA plays an important role in many eukaryotic processes, including translation, polyadenylation, splicing, mRNA export and mRNA decay. (ukri.org)
Preliminary data indicate that Dcs2 co-localizes with other members of the yeast mRNA decay pathway in P bodies. (ukri.org)

I then characterized in depth the functions of PARP1 and RBBP6 (implicated in DNA damage repair and cancer) unraveling their molecular mechanism of action within the context of pre-mRNA modification (specifically 3'-end cleavage and polyadenylation). (iit.it)
This suggests that coactivation of Lin28B and Follistatin may represent an endogenous mechanism mediating reprogramming of supporting cells for hair cell regeneration. (encyclopedia.pub)

These cells are regulated by a tight control of transcriptional programs. (biomed.news)

N 6 -methyladenosine modification destabilizes developmental regulators in embryonic stem cells. (nature.com)
The other universal modification found only in eukaryotic mRNA is a poly-A tail. (sciencing.com)
The present invention further relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells. (justia.com)

The SV40 late polyadenylation signal enables efficient cleavage and polyadenylation reactions resulting in high levels of steady-state mRNA. (invivogen.com)

Molecular biology of the cell (Sixth ed. (wikipedia.org)
Nature Reviews Molecular Cell Biology. (wikipedia.org)
He received his Ph.D. degree in the laboratory of Dr. En-Duo Wang in Biochemistry and Molecular Biology from Shanghai Institute of Biochemistry and Cell Biology of Chinese Academy of Science in 2008, where he studied the structural and functional interplay of transfer RNA (tRNA) and tRNA synthetase in bacteria, yeast and human. (rochester.edu)
In vitro systems with reproducible cell plasticity can help to identify relevant metabolic and molecular events during early cell reprogramming. (frontiersin.org)
In this context, we have been engaged in understanding the molecular events during muscle cell development in vertebrate embryos. (sorbonne-universite.fr)

This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. (cancerindex.org)
21 Furthermore, interaction between the BARD1/BRCA1 heterodimer and cleavage stimulation factor subunit 1 (CSTF1, also called CstF-50) represses the polyadenylation machinery, presumably to prevent inappropriate mRNA processing at sites of DNA repair. (bmj.com)

In eukaryotes, most lncRNAs are transcribed by RNA polymerase II and have a structure similar to that of mRNA, which includes 5′ capping, splicing and polyadenylation [ 2 ]. (biomedcentral.com)

Similar to polyadenylation, alternative splicing can occur, resulting in several possible proteins being translated from the same portion of DNA. (wikipedia.org)
thus, Pnn may affect crucial alternative splicing (AS) decisions and, in turn, impact cell-type specific gene expression. (molvis.org)
We used RNA-seq to analyze CPEB3-regulated alternative splicing on control and CPEB3-overexpressing cells. (aging-us.com)
Herein, we established that CPEB3 is a critical modulator of alternative splicing in neurogenesis, which remarkably enhances the current understanding of the CPEB3 mediated alternative pre-mRNA splicing. (aging-us.com)

Once phosphorylated and recruiting the polyadenylation complex, CPEB-1 may function as a translational activator stimulating polyadenylation and translation. (nih.gov)
CPEB-2 is an RNA-binding protein that is abundantly expressed in testis and localized in cytoplasm in transfected HeLa cells. (nih.gov)
CPEB-4 is overexpressed in a large variety of tumors and is associated with many mRNAs in cancer cells. (nih.gov)

During my PhD I worked in an RNA laboratory at Columbia University in New York where I was involved in the proteomic purification of the pre-mRNA 3'-end processing complex that led to the discovery of several novel proteins that connect mRNA processing with various nuclear pathways. (iit.it)
It is relatively well documented that transcription factors and signaling pathways are critically involved in the formation of inner ear structures and in the development of hair cells. (encyclopedia.pub)

It functions as a translational repressor that governs the synthesis of the AMPA receptor GluR2 through binding GluR2 mRNA. (nih.gov)
Transcription of a new pre-mRNA is terminated by hydrolytic cleavage in the 3'-UTR, and the newly formed 3'-end is protected from premature degradation by synthesis of a poly(A) tail. (uni-frankfurt.de)
[ 12 ] This mutation alters the polyadenylation site of the gene and results in increased mRNA synthesis, with a subsequent increase in protein expression. (medscape.com)

Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)
As a researcher in IIT I will develop original technologies and apply existing cutting edge genomics, proteomics, epigenetic engineering tools as well as advanced chromatin topology assays in cancer stem cells, in order to determine the role of ncRNAs and their epitranscriptional modifications in the organization of the 3D genome, with a specific focus on prom-enh loops. (iit.it)

Transfected cells can be analyzed for GFP expression by flow cytometry and by Western-blotting using GFP antibodies. (invivogen.com)

In mRNAs, rG4s are enriched in untranslated regions, and are significantly correlated with microRNA target sites and polyadenylation signals. (nih.gov)

By assessing mRNA profiles, it is possible to interrogate the specific genetic processes underlying, and giving rise to, specific phenotypes of interest. (biomedcentral.com)
GO and KEGG analyses indicated that numerous DE lncRNAs might be involved in diverse biological processes related to phosphate, such as lipid metabolic processes, catalytic activity, cell membrane formation, signal transduction, and nitrogen fixation. (biomedcentral.com)
Throughout my career I focused on understanding the cellular mechanisms underlying principles of gene expression and cell identity, aiming to shed light on the basic principles that govern these processes, both in physiological and pathological conditions. (iit.it)
Lin28A is required for hair cell regeneration in the mammalian cochlea, and may function in redundant processes with Lin28B [ 3 ] [ 5 ] . (encyclopedia.pub)

During egg activation, relative changes in poly(A)-tail length, and thus translational efficiency, were largely retained in the absence of cytoplasmic polyadenylation, which indicated that selective poly(A)-tail shortening primarily specifies these changes. (elifesciences.org)

Our results also revealed the presence of tail-length-independent mechanisms that maintained translation despite tail-length shortening during oocyte maturation, and prevented essentially all translation of bicoid and several other mRNAs before egg activation. (elifesciences.org)
We conclude that targeting key functional 3' end elements involved in pre-mRNA to mRNA maturation with antisense drugs can lead to efficient gene silencing and is thus a potentially effective therapeutic strategy for at least FSHD. (nih.gov)

2016. TH17 Cells are Preferentially Infected very Early after Vaginal Transmission of SIV in Macaques. (bu.edu)

The MZT marks the transfer of control of development from the mother to the zygote as maternal mRNAs are degraded, transcription from the zygotic genome begins, and embryonic development becomes dependent on zygotic gene products ( Tadros and Lipshitz, 2009 ). (elifesciences.org)
The construct was electroporated into R1 embryonic stem (ES) cells and correctly targeted ES cells were injected into recipient blastocysts. (jax.org)

Spermatogenesis produces haploid sperm capable of penetrating the oocyte, whereas oogenesis produces differentiated oocytes that are stockpiled with maternal nutrients, proteins, and mRNAs, and have outer layers that protect the embryo and enable fertilization. (elifesciences.org)

ADAR1 silencing in breast cancer cells leads to increased apoptosis, suggesting an anti-apoptotic function that promotes cancer progression. (cancerindex.org)
Stably introduced into cancer cell lines, the system reports on elevated endogenous ADAR1 editing activity induced by interferon as well as knockdown of ADAR1 and ADAR2. (cancerindex.org)
Mutant p53 shapes the enhancer landscape of cancer cells in response to chronic immune signaling. (nature.com)
Franco, H. L., Nagari, A. & Kraus, W. L. TNFα signaling exposes latent estrogen receptor binding sites to alter the breast cancer cell transcriptome. (nature.com)
Although the FDA-approved chimeric antigen receptor T-cell (CAR-T) therapies currently available today have provided a transformative new treatment option for some patients with cancer, these therapies have several significant drawbacks, such as the complexity of the manufacturing process, issues with reliability, and their limitation to treating hematological cancers. (cgtlive.com)
Our main focus in the laboratory is to understand why and how normal breast cells are transformed into cancer cells. (metu.edu.tr)
Beğik O, Öyken M, Çınkıllı T, Can T, Erson-Bensan AE , Alternative Polyadenylation Patterns for Novel Gene Discovery and Classification in Cancer. (metu.edu.tr)
Erson-Bensan AE , Can T. Alternative Polyadenylation: Another Foe in Cancer. (metu.edu.tr)
Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)

What Are Two Characteristics of mRNA in Eukaryotes? (sciencing.com)

Although widespread, the role of APA in the biology of cells, tissues, and organisms has been controversial. (stanford.edu)
Semaphorins are a wide family of extracellular regulatory signals with a multifaceted role in cell-cell communication. (biomed.news)
We have made the unprecedented finding that RBM24 plays a key role in cytoplasmic polyadenylation to control mRNA translation. (sorbonne-universite.fr)
Consistent with this activity, functional analyses in mice suggest that Lin28B plays an important role in hair cell development and regeneration. (encyclopedia.pub)
Studies using a zebrafish model further illustrate an important role of Lin28A in the recovery of progenitor cells. (encyclopedia.pub)

These data place Pnn at the fulcrum point between chromatin and mRNA splicing. (molvis.org)

2021) The YTHDF proteins ECT2 and ECT3 bind largely overlapping target sets and influence target mRNA abundance, not alternative polyadenylation. (oeaw.ac.at)
Brodersen P, Arribas-Hernández L, ..., Nodine MD (2021) The Arabidopsis m6A-binding proteins ECT2 and ECT3 bind largely overlapping mRNA target sets and influence target mRNA abundance, not alternative polyadenylation. (oeaw.ac.at)
More recently, as a research associate at Cornell I expanded this line of research to patient-derived glioma stem cells (GSC) building the first atlas of 3D promoter-enhancer (prom-enh) contacts in order to identify and target tumor-specific 3D enhancer hubs (manuscript in preparation). (iit.it)
In conclusion, TP53 was identified as a target of miR‑125b, and the minor allele of the rs78378222 polymorphism promoted the miR-125b/TP53 mRNA interaction. (spandidos-publications.com)

Developmentally regulated alternate 3' end cleavage of nascent transcripts controls dynamic changes in protein expression in an adult stem cell lineage. (stanford.edu)
Additionally, transfection with 50 nM miR‑125b mimics markedly reduced the mRNA and protein expression levels of TP53 in the cultured lens epithelial cells, and miR‑125b significantly induced apoptosis in the epithelial cells compared with negative control cells. (spandidos-publications.com)

In addition, the effects of reduced Bard1 expression have been studied in murine mammary epithelial cell cultures, where it was associated with complex cellular changes suggestive of a premalignant phenotype. (bmj.com)

Anatomy8

Organisms8

Chemicals and Drugs79

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes62

Information Science3

Cytoplasmic polyade1

Nucleus11

Tend to express mRNAs2

Stability12

Regulation16

UTRs4

Neurons2

Dynamic mRNA1

Regulates6

Genes11

Isoforms7

Control of mRNA1

Degradation of mRNAs1

Precursors1

Nascent mRNA1

Eukaryotic mRNAs3

Mediates1

Mature mRNA5

Regulate4

Proliferation3

Molecule4

Abstract1

Translational control1

Molecules3

Differentiation6

Sequences2

Mouse embryonic1

Decay3

Mechanism2

Transcriptional1

Modification3

SV401

Molecular5

Subunit2

Capping1

Alternative Splicing4

CPEB3

Pathways2

Synthesis3

Assays2

Antibodies1

MicroRNA1

Processes4

Efficiency1

Maturation2

Preferentially1

Embryonic2

Oocytes1

Cancer9

Eukaryotes1

Role5

Chromatin1

Target4

Protein expression2

Epithelial cell1