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  • liquid chromatograp
  • Quantitative proteome analysis of oral tongue squamous cell carcinoma by laser capture microdissection, stable isotope labeling and liquid chromatography-mass spectrometry. (aacrjournals.org)
  • The protein expression profiles of dissected normal/cancer cells were examined using a quantitative proteomics approach based on stable isotope labeling and liquid chromatography-mass spectrometry (LC-MS). Both protein extracts were reduced, alkylated and digested with trypsin. (aacrjournals.org)
  • analyses
  • Because bulk analyses of cells and gene expression - ignoring these regenerative units - have failed to illuminate the interactions between cells participating in these processes ( 4 , 5 ), there is growing interest in the spatial sampling of the cells within regenerative units. (frontiersin.org)
  • A custom-made chamber enables time-lapse topography and pre-selection of cell targets in order to minimize microdissection time, optimizing the quality of biomolecules for downstream analyses. (luriechildrens.org)
  • The method is widely applicable for microgenomics and microproteomics analyses in phenotypically evolving 3D cultures (i.e., of stem cells), under spontaneous or directed differentiation. (luriechildrens.org)
  • Our LCM-microarray analyses identified a set of genes associated with senescence which were significantly upregulated in senescent vs non-senescent cells in the human annulus. (biomedcentral.com)
  • However, as the corresponding cells are deeply embedded within the floral tissues, they are very difficult to access for experimental analyses, including cell-type specific transcriptomics. (uzh.ch)
  • Using cell-type-specific transcriptome analyses in a rodent model of chronic dopamine depletion, we identified large-scale gene expression changes, including neurotransmitter receptors, signal transduction cascades, and target proteins of dopamine signaling in striatonigral and striatopallidal neurons. (jneurosci.org)
  • For example, heterogeneous samples, rare cell types, cell lineage relationships, mosaicism of somatic tissues, analyses of microbes that cannot be cultured, and disease evolution can all be elucidated through single cell sequencing. (wikipedia.org)
  • Methods
  • While the methods described here are generally applicable to a wide range of species and cell types, our research focuses on plant reproduction. (uzh.ch)
  • Usually a mix of millions of cells are used in sequencing the DNA or RNA using traditional methods like Sanger sequencing or Illumina sequencing. (wikipedia.org)
  • The first step in quantifying the transcriptome is to convert RNA to cDNA using reverse transcriptase so that the contents of the cell can be sequenced using NGS methods as was done in genomics. (wikipedia.org)
  • There is currently no standardized technique to generate single-cell data, all methods must include cell isolation from the population, lysate formation, amplification through reverse transcription and quantification of expression levels. (wikipedia.org)
  • These methods include: Micropipetting Cytoplasmic aspiration Laser capture microdissection. (wikipedia.org)
  • analyze
  • FC is a multi-dimensional high-throughput technology that can analyze and sort individual cells based on their phenotypic characteristics - in most cases, sorting is based on cell surface molecule expression ( 7 , 8 ). (frontiersin.org)
  • His team also used laser microdissection on liver sections to detect HCV RNA, analyze HCV quasispecies and carry out liver proteomic analysis. (wikipedia.org)
  • proteomics
  • In the field of cellular biology, single-cell analysis is the study of genomics, transcriptomics, proteomics and metabolomics at the single cell level. (wikipedia.org)
  • Core facilities administered through IMMAG include: Cell imaging: fluorescence, confocal, and multiphoton microscopy Transgenic Mouse & Embryonic Stem Cell Transgenic Zebrafish Flow Cytometry Laser Capture Microdissection Electron Microscopy & Histology Proteomics & Mass Spectrometry Funding for IMMAG comes from sources such as the National Institute of Health, the United States Department of Energy, the United States Department of Defense, the Department of Veteran Affairs, and the National Aeronautics & Space Administration. (wikipedia.org)
  • cellular
  • Single cell sequencing examines the sequence information from individual cells with optimized next generation sequencing (NGS) technologies, providing a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment. (wikipedia.org)
  • By using deep sequencing of DNA and RNA from a single cell, cellular functions can be investigated extensively. (wikipedia.org)
  • Lastly, when examining cellular progression through differentiation, average expression profiles are only able to order cells by time rather than their stage of development and are consequently unable to show trends in gene expression levels specific to certain stages. (wikipedia.org)
  • genes
  • LCM of networks and randomly oriented cells followed by real-time PCR for select genes revealed that differential expression was time-dependent and increased with network maturity. (luriechildrens.org)
  • Additional genes related to cytokines, cell proliferation, and other processes were also identified. (biomedcentral.com)
  • Other genes, including genes associated with cell proliferation, extracellular matrix formation, cell signaling and other cell functions also showed significant modulation in senescent vs non-senescent cells. (biomedcentral.com)
  • While LAM in combination with transcriptional profiling allows the identification of genes and pathways active in any cell type with high specificity, establishing a suitable protocol can be challenging. (uzh.ch)
  • The purpose of single cell transcriptomics is to determine what genes are being expressed in each cell. (wikipedia.org)
  • Single-cell transcriptomics examines the gene expression level of individual cells in a given population by simultaneously measuring the messenger RNA (mRNA) concentration of hundreds to thousands of genes. (wikipedia.org)
  • tissues
  • The initial attempts to refine the bulk analysis of cells within regenerating tissues used flow cytometry (FC) ( 6 ). (frontiersin.org)
  • The epithelial tissues are formed by cells that cover the organ surfaces such as the surface of skin, the airways, the reproductive tract, and the inner lining of the digestive tract. (wikipedia.org)
  • These genomic studies are limited as they provide measurements for whole tissues and as a result show an average expression profile for all the constituent cells. (wikipedia.org)
  • Histology is the study of the microscopic anatomy (microanatomy) of cells and tissues of plants and animals. (wikipedia.org)
  • It is commonly performed by examining cells and tissues under a light microscope or electron microscope, the specimen having been sectioned (cut into a thin cross section with a microtome), stained, and mounted on a microscope slide. (wikipedia.org)
  • These fixatives preserve tissues or cells mainly by irreversibly cross-linking proteins. (wikipedia.org)
  • molecular
  • Thus, prolactin cell specific molecular events involved in pituitary tumorigenesis and cell signaling can be approached by proteomic analysis. (youscribe.com)
  • The understanding of developmental processes at the molecular level requires insights into transcriptional regulation, and thus the transcriptome, at the level of individual cell types. (uzh.ch)
  • Molecular analysis of cells from cytology specimens can help to establish a diagnosis in ambiguous cases. (bmj.com)
  • Molecular analysis of single cells circumvents this problem. (bmj.com)
  • To gain a deeper understanding of the role B cells play in this tumor family, we characterized the immune cell infiltrate of all three germ cell tumor subtypes and defined the molecular characteristics of the B cell Ag receptor expressed by tumor-associated B cells. (jimmunol.org)
  • Molecular characterization of the Ig variable region from 320 sequences expressed by germ cell tumor-infiltrating B cells revealed clear evidence of Ag experience, in that the cardinal features of an Ag-driven B cell response were present: significant somatic mutation, isotype switching, and codon insertion/deletion. (jimmunol.org)
  • Molecular characterization of the Ab repertoire revealed that the tumor-infiltrating B cells had undergone clonal expansion and isotype switching and had accumulated somatic mutations, all cardinal features of an Ag-driven B cell response. (jimmunol.org)
  • The main focus of Christian Bréchot's research is viral hepatitis B (HBV) and C (HCV), in particular their link with liver cancer (hepatocellular carcinoma, HCC), as well as the molecular mechanisms underpinning liver regeneration and cancer (especially the deregulation of the cell cycle). (wikipedia.org)
  • His work combines academic and clinical approaches in molecular virology and cell biology. (wikipedia.org)
  • characterization
  • A novel technique is presented for the monitoring and morphological characterization of 3D cell cultures targeted for laser capture microdissection (LCM). (luriechildrens.org)
  • This characterization also revealed the presence of both B cell clonal expansion and variation, suggesting that local B cell maturation most likely occurs within the tumor microenvironment. (jimmunol.org)
  • Because characterization of the immune cell infiltrate of germ cell tumors has thus far been limited to immunohistochemical analysis, the nature of the B cell response within the tumor microenvironment is not well understood. (jimmunol.org)
  • Some of the possible clinical applications for this technique include phasing of multiple mutations when parental samples are unavailable, preimplantation genetic diagnosis, prenatal diagnosis and in the characterization of cancer cells. (wikipedia.org)
  • microfluidic
  • Microfluidic whole genome haplotyping is a technique for the physical separation of individual chromosomes from a metaphase cell followed by direct resolution of the haplotype for each allele. (wikipedia.org)
  • colon cancer
  • Like several other tumor types, including melanoma, sarcoma, colon cancer, and breast cancer ( 2 ), germ cell tumors are often characterized by the presence of tumor-infiltrating lymphocytes (TIL). (jimmunol.org)
  • heterogeneous
  • However, mutations in heterogeneous samples might not be detected because of the diluting effect of DNA from normal background cells. (bmj.com)
  • enables
  • One popular method used for single cell genome sequencing is multiple displacement amplification and this enables research into various areas such as microbial genetics, ecology and infectious diseases. (wikipedia.org)
  • epithelial cell
  • Dr. Gordon's laboratory has investigated epithelial cell interaction with human-associated pathogens, including uropathogenic Escherichia coli, Helicobacter pylori, and Listeria monocytogenes. (wikipedia.org)
  • genomics
  • Single-cell genomics is heavily dependent on increasing the copies of DNA found in the cell so there is enough to be sequenced. (wikipedia.org)
  • Single-cell transcriptomics uses very similar techniques as single cell genomics. (wikipedia.org)
  • Once converted, there is not enough cDNA to be sequenced so the same DNA amplification techniques discussed in single cell genomics are applied to the cDNA to make sequencing possible. (wikipedia.org)
  • microscope
  • Elliott K, Hill DS, Lambert C, Burroughes TR, Gill P (2003) Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides. (springer.com)
  • Next, the cells are dropped onto a microscope slide so that the nucleus, which holds all of the genetic material together, breaks apart and releases the chromosomes onto the slide. (wikipedia.org)
  • Microdissection refers to a variety of techniques where a microscope is used to assist in dissection. (wikipedia.org)
  • Laser microdissection - use of a laser through a microscope to dissect selected cells. (wikipedia.org)
  • Manual single cell picking is a method is where cells in a suspension are viewed under a microscope, and individually picked using a micropipette. (wikipedia.org)
  • Currently six primary isolation/collection technologies exist using a microscope and device for cell isolation. (wikipedia.org)
  • Most are primarily dedicated micro-dissection systems, and some can be used as research microscopes as well, only one technology (#2 here, Leica) uses an upright microscope, limiting some of the sample handling capabilities somewhat, especially for live cell work. (wikipedia.org)
  • method
  • Optical Tweezers are also a contact-free method that uses a highly focused laser beam to manipulate and sort cells in suspension, for example bacteria. (zeiss.com)
  • Single cell sequencing was selected as the method of the year 2013 by Nature Publishing Group. (wikipedia.org)
  • transcriptome
  • The development of new technologies are increasing our ability to sequence the genome, and transcriptome, of single cells, as well as to quantify their proteome and metabolome. (wikipedia.org)
  • analysis
  • Here, we demonstrate an approach to conduct laser microdissection of the lung alveolar duct for single-cell PCR analysis. (frontiersin.org)
  • The ability to harvest anatomic units, such as the alveolar duct, under physiologic conditions has enabled detailed single-cell analysis. (frontiersin.org)
  • Zhao XC, Wang L, Sun J, Jiang BW, Zhang EL, Ye J (2016) Isolating sperm from cell mixtures using magnetic beads coupled with an anti-PH-20 antibody for forensic DNA analysis. (springer.com)
  • Our analysis, for example the RNA quality of the specimens, showed that the quality of the results is not influenced by the staining process and laser microdissection. (leica-microsystems.com)
  • This non-contact laser microdissection system makes cell isolation for further genetic analysis possible. (wikipedia.org)
  • Like typical NGS experiments, the protocols of single cell sequencing generally contain the following steps: isolation of a single cell, nucleic acid extraction and amplification, sequencing library preparation, sequencing and bioinformatic data analysis. (wikipedia.org)
  • A retrospective analysis performed in 2015 detailed the outcomes of N=148 men with non-obstructive azoospermia and diagnosed Sertoli cell-only syndrome: Men with SCOS: 148 Testicular sperm was successfully retrieved: 35/148 Successful ICSI: 20/148 Clinical pregnancy: 4/148 This study considers the effect of FSH levels on clinical success, and it excludes abnormal karyotypes. (wikipedia.org)
  • New developments in mass spectrometry techniques have become important analytical tools for proteomic and metabolomic analysis of single cells. (wikipedia.org)
  • In this technique, the cells or particles are trapped in a particular region for single cell analysis (SCA) usually without any application of external force fields such as optical, electrical, magnetic or acoustic. (wikipedia.org)
  • transcriptomics
  • To circumvent this problem, we have established a workflow for LAM that reproducibly results in high RNA quality that is well suitable for transcriptomics, as exemplified here by the isolation of cells of the female germline in apomictic Boechera. (uzh.ch)
  • Whilst these breakthroughs in transcriptomics technologies have enabled the generation of single-cell transcriptomic data there are new computational and analytical challenges presented by the data produced. (wikipedia.org)
  • roles
  • RNA is a biological macromolecule made of sugars and nitrogenous bases that plays a number of crucial roles in all living cells. (wikipedia.org)
  • In multicellular organisms different cell types within the same population can have distinct roles and form subpopulations with different transcriptional profiles. (wikipedia.org)
  • patients
  • Publications] Yamashita K, Okayasu I, 他4名: 'Unique characteristics of rectal carcinoma cell lines derived from invasive carcinomas in ulcerative colitis patients. (nii.ac.jp)
  • The Sertoli cell-only syndrome patients normally have normal secondary male features and have normal- or small-sized testes. (wikipedia.org)
  • 2017). Predictive Factors of Successful Microdissection Testicular Sperm Extraction in Patients with Presumed Sertoli Cell-Only Syndrome. (wikipedia.org)
  • His research has focused on the impact of HBV DNA integration and the interactions between HCV proteins and the main cell signaling pathways, such as the interferon and TGF-beta pathways, contributing to improve the understanding of the mechanisms behind liver cancer in patients suffering from chronic HBV or HCV infection. (wikipedia.org)
  • functional
  • For instance, the accordance between the morphology and the functional classes of cat retinal ganglion cells has been studied to show the relationship between neuron shape and function. (wikipedia.org)
  • bulk
  • It is more challenging to perform single cell sequencing in comparison with sequencing from cells in bulk. (wikipedia.org)
  • These differences are missed by bulk sequencing of a population, but can be observed in single cell sequencing. (wikipedia.org)
  • Moreover, bulk assays fail to identify if a change in the expression profile is due to a change in regulation or composition, in which one cell type arises to dominate the population. (wikipedia.org)
  • interactions
  • nonetheless, the relatively large number of cells sorted by FC has potentially obscured the cell-cell interactions within individual regenerative units. (frontiersin.org)
  • A major criticism to RIN is when using with plants or in studies of eukaryotic-prokaryotic cells interactions. (wikipedia.org)
  • However, there is also a growing evidence for volume transmission, a process that involves electrochemical interactions from the whole cell membrane. (wikipedia.org)
  • single cell
  • The minimal amount of starting materials from a single cell make degradation, sample loss and contamination exert pronounced effects on quality of sequencing data. (wikipedia.org)
  • In addition, due to the picogram level of the amount of nucleic acids used, heavy amplification is often needed during sample preparation of single cell sequencing, resulting in the uneven coverage, noise and inaccurate quantification of sequencing data. (wikipedia.org)
  • Recent technical improvements make single cell sequencing a promising tool for approaching a set of seemingly inaccessible problems. (wikipedia.org)
  • Single cell DNA genome sequencing involves isolating a single cell, performing whole-genome-amplification (WGA), constructing sequencing libraries and then sequencing the DNA using a next-generation sequencer (ex. (wikipedia.org)
  • Some of the tools that can be used for single cell genome sequencing include: SPAdes, IDBA-UD, Cortex and HyDA. (wikipedia.org)
  • Two ways to improve genome coverage include: pooling single-cell MDA reactions from the same cell type and pooling before the reaction is performed. (wikipedia.org)
  • Single-nucleotide polymorphisms (SNPs), which are a big part of genetic variation in the human genome, and copy number variation (CNV), pose problems in single cell sequencing, as well as the limited amount of DNA extracted from a single cell. (wikipedia.org)
  • Various SNP algorithms are currently in use but none are specific to single cell sequencing. (wikipedia.org)
  • There are two major applications to studying the genome at the single cell level. (wikipedia.org)
  • These patterns of mutation can be observed using single cell sequencing. (wikipedia.org)
  • differentiation
  • Seminal plasma protein TEX101 was proposed for differentiation of Sertoli cell-only syndrome from maturation arrest and hypospermatogenesis. (wikipedia.org)
  • procure
  • Finally, few of these techniques are able to procure single cells. (bmj.com)
  • The cytopicker can rapidly and simply procure single cells, such as lymphoblasts, from cytology specimens, such as peripheral blood smears. (bmj.com)
  • integrity
  • Capturing the limited number of epithelial cells available in each MG sample is a race against time in order to preserve RNA integrity. (biomedcentral.com)
  • RIN has also been used to test the efficiency of other techniques, such as microdissections, on maintaining RNA integrity in human cells, so it has a wide variety of uses in testing the quality of new techniques. (wikipedia.org)
  • germ
  • Yet little is known about whether the humoral immune response affects germ cell tumor biology. (jimmunol.org)
  • Germ cell tumors principally occur in children and young adults. (jimmunol.org)
  • 3 A number of immunohistochemical studies have demonstrated that the immune cell infiltrate of many germ cell tumors includes substantial yet variable numbers of T cells, macrophages, and B cells ( 3 , 4 , 5 ). (jimmunol.org)
  • Indeed, the presence of substantial B cell numbers in the microenvironment of many germ cell tumors suggests a role for Ag-driven, Ab-mediated immunity or nonspecific lymphocyte recruitment by inflammatory and chemotactic cytokines. (jimmunol.org)
  • Del Castillo syndrome and germ cell aplasia ) is a disorder characterized by male sterility without sexual abnormality. (wikipedia.org)
  • On retrieval of viable sperm this could be used in Intracytoplasmic Sperm injection ICSI In 1979, Levin described germinal cell aplasia with focal spermatogenesis where a variable percentage of seminiferous tubules contain germ cells. (wikipedia.org)
  • subtypes
  • There are several subtypes of RNA, with the most prominent in the cell being tRNA (transfer RNA), rRNA (ribosomal RNA), and mRNA (messenger RNA). (wikipedia.org)
  • specific
  • Even if a mutation were detected, it could not be traced back to a specific cell type. (bmj.com)
  • Collectively, our results suggest that the tumor-associated B cell repertoire is most likely shaped through expression of specific Ag(s). (jimmunol.org)
  • Scalenghe F, Turco E, Edstrom JE, Pirrotta V, Melli M.: Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes. (wikipedia.org)
  • stimuli
  • The geometry of neurons often depends on the cell type and the history of received stimuli that is processed through the synapses. (wikipedia.org)
  • neurons
  • The Italian scientist Camillo Golgi grouped neurons into type I and type II cells. (wikipedia.org)
  • Golgi I neurons have long axons that can move signals over long distances, such as in Purkinje cells, whereas Golgi II neurons generally have shorter axons, such as granule cells, or are anoxonic. (wikipedia.org)
  • specimens
  • LCM was used to separately harvest senescent and non-senescent cells from 11 human annulus specimens. (biomedcentral.com)
  • Steel cannulae are optimal for microdissection of cells from the hard matrix of cytology specimens but aspirate poorly. (bmj.com)
  • Immunohistochemistry revealed a prominent B cell infiltrate in the microenvironment of all tumors examined and clear evidence of extranodal lymphoid follicles with germinal center-like architecture in a subset of specimens. (jimmunol.org)
  • several
  • Several ways to identify cells of the same strain are: fluorescent in situ hybridization (FISH) and conformation characteristics. (wikipedia.org)
  • Christian Bréchot's team of researchers has also shed light on several aspects related to cell cycle regulation, in particular through the identification of human cyclin A at the genomic insertion site of HBV in an HCC. (wikipedia.org)
  • neuron
  • It took nearly a century after the acceptance of the cell as the basic unit of life before researchers could agree upon the shape of a neuron. (wikipedia.org)
  • melanoma
  • It was shown previously that aggressive melanoma cells spontaneously differentiate on collagen gels into vascular-like networks with strong endogenous angiogenic potential. (luriechildrens.org)
  • Players
  • Key Players in the laser capture microdissection market include Carl Zeis AG, Molecular Machines & Industries, Osmium Biosolutions LLC, DeNova Sciences Pte Ltd, Danaher Corporation, Thermo Fisher Scientific Inc, Indivumed GmbH, AvanSci Bio L.L.C., Theranostics Health Inc and 3DHISTECH Ltd, among others. (lincolnnewsreporter.com)
  • chromosome
  • Leveraging his respected and senior status within the scientific community, Leibel obtained ongoing funding from the National Institutes of Health and other sources, allowing the team to develop and utilize new techniques in their research such as chromosome microdissection. (wikipedia.org)
  • laser
  • Utilizing laser microdissection, gene-microarray, qRT-PCR, and immunohistochemistry, we focused on the intrinsic post-axotomy response of specifically labelled thoracic propriospinal neurons at periods from 3-days to 1-month following T9 spinal cord injury. (beds.ac.uk)
  • Laser-capture microdissection was used to enrich the samples for lymphoma cells. (queensu.ca)
  • Technically, minuscule amounts of a) cellular lysates, from intact cells or laser capture microdissected cells, b) body fluids such as serum, CSF, urine, vitreous, saliva, etc., are immobilized on individual spots on a microarray that is then incubated with a single specific antibody to detect expression of the target protein across many samples. (wikipedia.org)
  • Patients
  • The epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKI) gefitinib and erlotinib benefit some non-small cell lung cancer (NSCLC) patients, but most do not respond (primary resistance) and those who initially respond eventually progress (acquired resistance). (pubmedcentralcanada.ca)
  • Epidermal growth factor receptor (EGFR) inhibitors have shown clinical benefit for only a subset of non-small cell lung cancer (NSCLC) patients, and even patients who do initially experience a major response eventually develop therapeutic resistance. (pubmedcentralcanada.ca)
  • The Sertoli cell-only syndrome patients normally have normal secondary male features and have normal- or small-sized testes. (wikipedia.org)
  • 2017). Predictive Factors of Successful Microdissection Testicular Sperm Extraction in Patients with Presumed Sertoli Cell-Only Syndrome. (wikipedia.org)
  • germ cells
  • On retrieval of viable sperm this could be used in Intracytoplasmic Sperm injection ICSI In 1979, Levin described germinal cell aplasia with focal spermatogenesis where a variable percentage of seminiferous tubules contain germ cells. (wikipedia.org)
  • Analysis showed that it completely lacked germ cells, which produce gametes. (wikipedia.org)
  • tumor cell
  • These studies suggest that erlotinib resistance may be associated with a rise in both tumor cell and host stromal VEGF and that combined blockade of the VEGFR and EGFR pathways can abrogate primary or acquired resistance to EGFR TKIs. (pubmedcentralcanada.ca)
  • regulation
  • The up-regulation in the expression of genes for several growth factor receptors during the first week post-SCI also suggest that administration of these factors may protect TPS neurons from cell death and maintain a regenerative response, but only if given during the early period after injury. (beds.ac.uk)
  • Because defects in the regulation of apoptotic cell death are fundamental in FL pathogenesis, we hypothesized that deregulated expression of components of the pRb signaling pathway may promote cell proliferation, thereby complementing antecedent anti-apoptotic mutations and producing more aggressive disease. (queensu.ca)
  • Gene regulation studies include the organization and evolution of the regulatory genome, chromatin composition and transcriptional regulation mediated by steroid hormones, epigenetic mechanisms in leukemia and stem cells, regulation of periodic splicing and mRNA translation, and gene function and epigenetic reprogramming in embryogenesis and the germline. (wikipedia.org)
  • Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. (wikipedia.org)
  • Motility promoting functions are related to the regulation of the movement of nerve cells during neural development. (wikipedia.org)
  • These interactions promote cell adhesion and regulation of signal transduction. (wikipedia.org)
  • neurons
  • The expression of a number of pro-apoptotic genes up-regulated at 3-days p.i. suggest that changes in gene expression after this period may have resulted from analyzing surviving TPS neurons after the cell death of the remainder of the axotomized TPS neuronal population. (beds.ac.uk)
  • Taken collectively these data demonstrate that thoracic propriospinal (TPS) neurons mount a very dynamic response following low thoracic axotomy that includes a strong regenerative response, but also results in the cell death of many axotomized TPS neurons in the first week after spinal cord injury. (beds.ac.uk)
  • It is also expressed in intestinal epithelial progenitor cells, cerebellum neurons such as Cerebellum granule cell and Purkinje cells. (wikipedia.org)
  • proteins
  • In addition, since RPMA can utilize whole-cell or undissected or microdissected cell lysates, it can provide direct quantifiable information concerning post translationally modified proteins that are not accessible with other high-throughput techniques. (wikipedia.org)
  • Because the principles that underlie the behavior of proteins within and across cells are poorly understood, the CDB program aims to study the interactions between intercellular signaling systems and the cytoskeleton. (wikipedia.org)
  • Signaling
  • Enhanced Glucocorticoid Receptor Signaling in T Cells Impacts Thymocyte Apoptosis and Adaptive Immune Responses The American Journal of Pathology. (jove.com)
  • Pubmed ID: 17322387 To study the effect of enhanced glucocorticoid signaling on T cells, we generated transgenic rats overexpressing a mutant glucocorticoid receptor with increased ligand affinity in the thymus. (jove.com)
  • study
  • Specific subcompartments of the respiratory system, obtained by microdissection, have been used to study the cytochrome P450-dependent metabolism of naphthalene and the epoxide hydrolase/glutathione transferase-dependent metabolism of naphthalene oxide. (aspetjournals.org)
  • A retrospective analysis performed in 2015 detailed the outcomes of N=148 men with non-obstructive azoospermia and diagnosed Sertoli cell-only syndrome: Men with SCOS: 148 Testicular sperm was successfully retrieved: 35/148 Successful ICSI: 20/148 Clinical pregnancy: 4/148 This study considers the effect of FSH levels on clinical success, and it excludes abnormal karyotypes. (wikipedia.org)
  • After graduation, Seifriz went to Geneva, Switzerland to study cell physiology with Robert Hippolyte Chodat. (wikipedia.org)
  • gene
  • The captured cells can then be used in a wide range of downstream assays, such as gene expression analysis, genomic profiling using microarrays, or for proteomic analysis. (robarts.ca)
  • immune
  • Collectively, our findings suggest that endogenous glucocorticoids impact T-cell development and favor the selection of Th2- over Th1-dominated adaptive immune responses. (jove.com)
  • hypothesis
  • We paid particular attention to changes related to their regenerative ability and to early cell loss, based on the hypothesis that the short distance from the lesion would ensure a maximal regenerative response. (beds.ac.uk)
  • sperm
  • Sertoli cell only syndrome is like other non-obstructive azoospermia (NOA) cases are managed by sperm retrieval through testicular sperm extraction (mTESE), micro-surgical testicular sperm extraction (mTESE), or testicular biopsy. (wikipedia.org)
  • molecules
  • At the end of the 1800s, the development of very thin and consistently thin samples by microtomy, together with the selective staining of important cell components or molecules allowed for the visualisation of microscope details. (wikipedia.org)
  • specifically
  • In addition, L1 participates in myelination processes, which are involved in the proliferation of myelin through the nervous system (specifically the progressive myelination of nerve axon fibers), by mediating the elongation of Schwann cells along the axon. (wikipedia.org)
  • stages
  • Non-small cell lung cancer (NSCLC) is the leading cause of cancer deaths in the United States ( 1 ) and worldwide, with a 5-year survival rate of only 15% for all stages combined ( 2 ). (pubmedcentralcanada.ca)
  • The experimental stages are listed below: Cells are grown in T-25 flasks at 37 degree and 5% CO2 in appropriate medium. (wikipedia.org)
  • survival
  • In addition, several regeneration associated and cell survival/neuroprotective genes were significantly up-regulated at the earliest p.i. period studied. (beds.ac.uk)
  • Human
  • Conclusions Differential DNA methylation at the PPARγ promoter can be detected within the pool of cell-free DNA of human plasma. (bmj.com)
  • Plant
  • William Seifriz (August 11, 1888 - July 13, 1955) was a Professor of Biology at the University of Pennsylvania and an important figure in the history of plant physiology and plant cell biology. (wikipedia.org)
  • Plasma
  • Plasma cell-free circulating DNA methylation of PPARγ was quantitatively assessed by pyrosequencing. (bmj.com)
  • made
  • Leptin (from Greek λεπτός leptos, "thin"), "the hormone of energy expenditure", is a hormone predominantly made by adipose cells that helps to regulate energy balance by inhibiting hunger. (wikipedia.org)
  • normally
  • Due to homeostatic proliferation, a considerable number of mature T lymphocytes accumulated in the periphery, responding normally to costimulation but exhibiting a perturbed T-cell repertoire. (jove.com)
  • presence
  • Sertoli cell only syndrome is likely multifactorial, and characterized by severely reduced or absent spermatogenesis despite the presence of both Sertoli and Leydig cells. (wikipedia.org)
  • surface
  • Boron-doped zinc oxide (BZO) films, deposited by metal-organic chemical vapor deposition (MOCVD), have been widely used as front electrodes in thin-film solar cells due to their native pyramidal surface structure, which results in efficient light trapping. (jove.com)
  • levels
  • Polyunsaturated liposomes are antiviral against hepatitis B and C viruses and HIV by decreasing cholesterol levels in infected cells. (blogspot.com)
  • Transcriptome profiling is determining the composition of transcripts and their relative expression levels in a given reference set of cells. (wikipedia.org)
  • maintain
  • In order to increase the Jsc and maintain the improved electrical properties (Voc, FF) of the cell, a thin BZO coating, deposited by MOCVD, was introduced to coat the sputtering-treated BZO film. (jove.com)