• We used VES and FBZ, at low concentrations, singly and in combination, to test their inhibitory effects on proliferation of human and mouse prostate cancer cells in vitro. (researchgate.net)
  • Administered alone, FBZ inhibited proliferation faster than VES in both mouse and human prostate cancer cell lines and a synergistic effect between both was also observed. (researchgate.net)
  • The results showed that MMNC inhibits the proliferation of HCT116 and Caco-2 cells, blocks the cell cycle in the G2/M phase, decreases the cell mitochondrial membrane potential and induces apoptosis. (bvsalud.org)
  • Sphingolipids are an extensive class of lipids with different functions in the cell, ranging from proliferation to cell death. (frontiersin.org)
  • High level of RRS1 in breast cancer cell lines is associated with increased cell proliferation, invasion and migration. (morulaivf.com)
  • In the present study, by silencing or overexpressing JMJD5 in pancreatic cancer cells, we examined the impact of JMJD5 on cell proliferation and glucose metabolism. (morulaivf.com)
  • JMJD5 loss promoted pancreatic cancer cell proliferation and induced a cellular metabolic shift from oxidative phosphorylation to glycolysis. (morulaivf.com)
  • Overall, the present study indicated that decreased JMJD5 expression promoted cell proliferation and glycolytic metabolism in pancreatic cancer cells in a c-Myc-dependent manner. (morulaivf.com)
  • These cellular components can include cells of the innate immune system, such as myeloid-derived suppressor cells (MDSCs), tumor-associated macrophages (TAMs), and eosinophils, as well as cells of the adaptive immune system, including CD4⁺ T regulatory cells (Tregs) and cytotoxic CD8⁺ T cells. (ubc.ca)
  • I found that eosinophils were directly cytotoxic towards mammary carcinoma cells, likely through degranulation, and that eosinophils decreased pulmonary metastatic growth of breast cancer cells. (ubc.ca)
  • Ongoing research investigates mechanisms invoked by cancer cells to abrogate MHC-I expression and attenuate anti-tumour CD8 + cytotoxic T cell response. (portlandpress.com)
  • Tumor-specific neoantigens, which are expressed on tumor cells, can induce an effective antitumor cytotoxic T-cell response and mediate tumor regression. (wjgnet.com)
  • A ligand binding assay (LBA) is an assay, or an analytic procedure, which relies on the binding of ligand molecules to receptors, antibodies or other macromolecules. (wikipedia.org)
  • The foundations for which ligand binding assay have been built are a result of Karl Landsteiner, in 1945, and his work on immunization of animals through the production of antibodies for certain proteins. (wikipedia.org)
  • We adopted a distinct approach aimed initially at interfering with protein-protein interactions of RAS, and other so-called undruggable targets like transcription factors, using molecular biology tools called intracellular antibodies that bind to target proteins inside cells. (cancer.gov)
  • The former issue was often neglected prior to initiating drug discovery programmes while the latter was considered impossible because it was purported that antibodies were unsuitable to derive compounds. (cancer.gov)
  • These drugs are designed to break down the protein "checkpoints" that cancer cells create to protect themselves from antibodies that seek to destroy them. (timesofisrael.com)
  • Specifically, despite the human body's endogenous receptors, hormones, and other neurotransmitters, pharmacologists utilize assays in order to create drugs that are selective, or mimic, the endogenously found cellular components. (wikipedia.org)
  • Such advances provide researchers with the ability not only to quantify hormones and hormone receptors, but also to contribute important pharmacological information in drug development and treatment plans. (wikipedia.org)
  • Radioligands are used to measure the ligand binding to receptors and should ideally have high affinity, low non-specific binding, high specific activity to detect low receptor densities, and receptor specificity. (wikipedia.org)
  • T-cell and CD4 receptors on the surface of T cells bind to the HLA protein/T-cell epitope complex in order to generate an immune response. (genengnews.com)
  • Pardoll has a particular interest in cancer immunology and his lab's studies on basic immunologic mechanisms have led to the development and design of a number of cancer vaccines and discovery of key checkpoint ligands and receptors, such as PD-L2, LAG-3 and neuritin, many of which are being targeted clinically. (hopkinsmedicine.org)
  • The anti-PD1 agent given in ICI therapy prevents the binding of the ligands and receptors and boosts the body's immune response. (timesofisrael.com)
  • But what's more important is the functionality of the binding of the ligands and receptors. (timesofisrael.com)
  • The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. (cancerindex.org)
  • The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. (cancerindex.org)
  • This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)
  • I demonstrated that tumor cells secreted proteins that drove the recruitment and/or expansion of pulmonary TAMs, Tregs, and granulocytic-MDSCs (G-MDSCs), resulting in increased primary lung tumor growth and pulmonary metastasis of mammary carcinoma cells, respectively. (ubc.ca)
  • Cancer cells can exploit normal mechanisms of cellular motility to facilitate invasion and metastasis, the process by which cancer cells disseminate to distant organs. (ubc.ca)
  • In this study, lentiviral transfection of sh‑RNA was used to knock down the level of RRS1, to detect the effect of RRS1 on cell function and to explore the specific mechanism of RRS1 affecting cell invasion and metastasis by COIP and dual‑luciferase reporter gene assays. (morulaivf.com)
  • This inhibited trans‑activation of SNAIL by c‑Myc and eventually decreased the invasion and metastasis capacity of the human breast cancer cell line BT549. (morulaivf.com)
  • Taken together, RRS1 regulates invasion and metastasis of human breast cancer cells through the RPL11‑c‑Myc‑SNAIL axis. (morulaivf.com)
  • Dr. Welm completed her PhD in Cell and Molecular Biology at Baylor College of Medicine in Houston, TX under the supervision of Gretchen Darlington, PhD. She then went on to conduct postdoctoral training in Dr. J. Michael Bishop's laboratory at the University of California, San Francisco where her work focused on developing new models of breast cancer metastasis. (utah.edu)
  • Dr. Welm's group discovered that the Ron kinase pathway is an important facilitator of breast cancer metastasis through its unique dual function in tumor cells and in resident macrophages. (utah.edu)
  • As a consequence of treatments, the most frequent mechanism of cell death is apoptosis, followed by autophagy. (frontiersin.org)
  • Sphingolipids are remarkably diverse and have crucial roles in maintaining barrier function and fluidity, as well as regulating the cell cycle, cell motility, differentiation, adhesion, and apoptosis ( 1 ). (frontiersin.org)
  • To investigate the effect of gambogic acid (GA) on apoptosis in the HT-29 human colon cancer cell line. (wjgnet.com)
  • Cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling and Hoechst 33342 staining, and quantified by flow cytometry. (wjgnet.com)
  • Furthermore, two HPV oncoproteins, E6 and E7, caused carcinogenesis by targeting various cellular pathways, including HPV DNA integration with host DNA, progression, disturbance of cell-cycle checkpoints, and apoptosis.Despite the success of CC prevention vaccines, therapy for the disease is significantly less satisfactory because of multidrug resistance and side effects. (pdfplayer.org)
  • LN metastases resist T cell-mediated cytotoxicity, induce antigen-specific regulatory T cells, and generate tumor-specific immune tolerance that subsequently facilitates distant tumor colonization. (stanford.edu)
  • When loaded with immune complexes (IC), consisting of tumor antigens bound to antitumor antibody, BMDC induce powerful antitumor immunity in mice. (stanford.edu)
  • One such biological target was RAS, where decades of attempts had failed (until very recently) to yield anti-cancer drugs directly interfering with RAS function. (cancer.gov)
  • Biological assays to test the cytotoxicity of the compound 1b combined with electroporation were performed to determine its potential for future medical applications in cancer treatment. (hindawi.com)
  • Pharmacophore-based activity profiling screen demonstrated some biological targets that MS44-53 may modulate their biological response, and thus can be considered as potential drugs to treat different kinds of diseases, such as carcinoma, diabetes type II, bacterial infection and cardiovascular diseases. (openmedicinalchemistryjournal.com)
  • Wnt/β-catenin signaling is a branch of a functional network that is involved in a broad range of biological systems including stem cells, embryonic development and adult organs. (chemdiv.com)
  • Deregulation of Jumonji-C domain-containing protein 5 (JMJD5) has been associated with multiple facets of biological processes in cancer cells. (morulaivf.com)
  • The biological activity of HTS02 is assessed by a tubulin polymerization assay. (cytoskeleton.com)
  • Protein markers are an essential component in biological research and drug development. (acrobiosystems.com)
  • The active compound curcumin is believed to have a wide range of biological effects including anti-inflammatory, antioxidant, antitumor, antibacterial, and antiviral activities, which have shown a lot of potential for use in clinical medicine. (blogspot.com)
  • This library represents a selection of drug-like compounds aimed at modulating protein-protein interaction (PPI) of β-catenin with different proteins involved in significant physiological processes. (chemdiv.com)
  • In Chapter 2, I examined how proteins secreted by cancer cells can promote a tumor-permissive microenvironment through the recruitment of suppressive innate and adaptive immune cells to the lungs. (ubc.ca)
  • Targeting tumor-secreted proteins, or the suppressive immune cells that are expanded in response to said proteins, led to a decrease in primary and metastatic pulmonary disease. (ubc.ca)
  • This switching off happens when the PD1 receptor binds to the other cells' PDL1 or PDL2 ligands (also proteins). (timesofisrael.com)
  • The aim of this thesis work is to interrogate the pro- and anti- tumorigenic roles myeloid cells play in primary and metastatic disease, and how these myeloid populations interact with cells of the adaptive immune system to influence anti-tumor immunity. (ubc.ca)
  • Secarna Pharmaceuticals, an IZB-based biopharmaceutical company focusing on the discovery and development of next-generation antisense oligonucleotide (ASO) therapies to address challenging or previously undruggable targets via its LNAplus™ platform, announced the publication of striking new preclinical data demonstrating that bimodal ASOs can enable efficacious long-term antitumor immunity in tumor models compared to current immune checkpoint inhibitors. (izb-online.de)
  • Collectively, these data show that IM-T9P1 leads to long-lasting, adaptive antitumor immunity that can be further improved when combined with T cell-targeting immune checkpoint inhibitors and can have efficacy against large, established tumors in preclinical tumor models. (izb-online.de)
  • Treatment with IM-T9P1 resulted in the sustained activation of a specific type of tumor infiltrating cell, dendritic cells 3 (DC3s), which can promote antitumor immunity by supporting T cells with survival signals and enabling them to perform their effector functions. (izb-online.de)
  • These results demonstrate the importance of DCs in overcoming ICI resistance and achieving effective and durable antitumor responses and, in many cases, antitumor immunity. (izb-online.de)
  • However, this finding has triggered further interest in utilising tumour-specific MHC-II to harness sustained anti-tumour immunity through the activation of CD4 + T helper cells. (portlandpress.com)
  • Our main goal is to identify new tumor antigens specifically expressed at the tumor cell surface and to validate their potential use as new therapeutic targets through the development of antibody-based immune-therapies. (hipi-lab-saint-louis.fr)
  • Although malignant cells were identified as a clonal expansion of CD4+ T cell with memory phenotype, their phenotypic detection relies on the loss of pan-T cell antigens such as CD26 or/and CD7. (hipi-lab-saint-louis.fr)
  • Because 10% of Sézary patients display malignant T cells that do not express KIR3DL2, we pursue our search for specific tumor antigens. (hipi-lab-saint-louis.fr)
  • Gp120 binds to cells expressing CD4 cell-surface antigens, most notably T4-lymphocytes and monocytes/macrophages. (lookformedical.com)
  • The N-terminal part of gp41 is thought to be involved in CELL FUSION with the CD4 ANTIGENS of T4 LYMPHOCYTES, leading to syncytial formation. (lookformedical.com)
  • These drugs may deserve to be tested for their efficacy in the control of prostate cancer using in vivo models. (researchgate.net)
  • It has been proven that combining electroporation with chemotherapy potentiates the cytotoxicity of drugs when the drugs' efficacy is limited by its uptake in the cell [ 9 - 13 ]. (hindawi.com)
  • A cholesterol-tethered platinum ii-based supramolecular nanoparticle increases antitumor efficacy and reduces nephrotoxicity. (cetbwh.org)
  • Accordingly, they illuminated new uses for IDO1 inhibitors to enhance the antitumor efficacy of hypoxia/metabolic stress-inducing drugs in cancer and other diseases. (mainlinehealth.org)
  • Combination drug studies using IDO inhibitors to safely enhance the efficacy of cancer cell-targeted therapeutics, including RAS/RAF pathway inhibitors, PI3K/mTOR pathway inhibitors and hypoxia-inducing agents. (mainlinehealth.org)
  • Co-inhibition of IGF and CHK1 caused synergistic suppression of cell viability, cell survival and tumour growth in 2D cell culture, 3D spheroid cultures and in vivo. (nature.com)
  • By performing ex vivo experiments on circulating cells from Sézary patients, we established that KIR3DL2 was functional on tumor cells and acted as an inhibitory co-receptor. (hipi-lab-saint-louis.fr)
  • Our in vitro, ex vivo and in vivo results demonstrated that such antibody was able to promote depletion of the malignant cells by antibody-dependent cell-cytotoxicity or -phagocytosis. (hipi-lab-saint-louis.fr)
  • I demonstrated that using glucose restriction of CD8⁺ T cells in vitro led to improved tumor regression in vivo. (ubc.ca)
  • Peptides R, S and I were chosen for evaluation in in vivo models of lung metastases (B16-CXCR4 and KTM2 murine osteosarcoma cells) and growth of a renal cells xenograft. (cnr.it)
  • Sphingolipid-targeting drugs have been tested alone or in combination with chemotherapy, exhibiting antitumor activity alone and in synergism with chemotherapy in vitro and in vivo . (frontiersin.org)
  • Predicting clinical response to anticancer drugs using an ex vivo platform that captures tumour heterogeneity. (cetbwh.org)
  • Drug repositioning and pharmacophore identification in the discovery of hookworm MIF inhibitors. (jscimedcentral.com)
  • the development of strategies by tumor cells to adapt and escape to antibody or protein inhibitors-based therapies. (hipi-lab-saint-louis.fr)
  • Tubulin polymerization must also be responsive to polymerization enhancers (paclitaxel) and inhibitors (nocodazole) at 5 µM drug concentration. (cytoskeleton.com)
  • Ying-Ting Lin*, Kuo-Jung Huang, Chin-kai Tseng, Kuan-Jen Chen, Hui-Min Wang, and Jin-Ching Lee, "Efficient In Silico Assay of Inhibitors of Hepatitis C Virus RNA-Dependent RNA Polymerase by Structure-Based Virtual Screening and In Vitro Evaluation. (kmu.edu.tw)
  • Thus, there is a need for drug adjuvants to leverage the immunomodulatory effects of targeted drugs where IDO inhibitors are well-positioned. (mainlinehealth.org)
  • New uses of IDO inhibitors in combination drug therapy: PCT patent application. (mainlinehealth.org)
  • Molecular defects are often represented by hereditary BRCA (Breast cancer gene) mutations leading to altered DNA repair, thus presuming higher efficiency of DNA-damaging agents, such as platinum drugs and poly-ADP-ribose polymerase (PARP) inhibitors. (cttjournal.com)
  • Second, we collected 56 targets closely related to the occurrence and development of cancer, metabolic diseases, and inflammatory immune diseases and developed a multi-model self-validation activity prediction (MSAP) framework that enables accurate bioactivity quantification predictions for small-molecule ligands of these 56 targets. (bvsalud.org)
  • ists, assay developers, pharmacologists, toxicologists and pharmacokinetic/drug metabolism experts, who identify novel drug targets arising from JHU faculty's research and translate them into new, small molecule drug therapies. (hopkinsmedicine.org)
  • With over 50 compounds in development and 40 disease areas being studied, diverse and unique career opportunities are available in areas from Small Molecule Drug Discovery to Nonclinical, and Translational Research. (bms.com)
  • however, the developing of small-molecule-based fluorescent binding ligands that are highly selective in targeting these G4-structures over other types of nucleic acids is challenging. (morulaivf.com)
  • These tools were initially used in RAS target validation and subsequently for isolating drug leads, guided by the intracellular antibody interaction with RAS. (cancer.gov)
  • We exploited the specific binding of the antibody fragment and were able to identify RAS-binding compounds in antibody-competition assays (illustrated in Fig.1). (cancer.gov)
  • The latter discovery was important for us because it suggests that the antibody combining sites could generally be accessible for implementing the Abd technology approach to drug discovery. (cancer.gov)
  • Aiming to exploit this effect in therapy we performed a compound screen in five breast cancer cell lines with IGF neutralising antibody xentuzumab. (nature.com)
  • It involves introducing monoclonal antibody drugs via infusion into a vein. (timesofisrael.com)
  • Computationally designed antibody-drug conjugates self-assembled via affinity ligands. (cetbwh.org)
  • Development of a facile antibody-drug conjugate platform for increased stability and homogeneity. (cetbwh.org)
  • Setting the corresponding isotype control antibody to detect non-specific binding can reduce the generation of false positive results and evaluate the possible influencing factors accurately in the drug development process. (acrobiosystems.com)
  • 50 years of preclinical anticancer drug screening:empirical to target-driven approaches. (jscimedcentral.com)
  • The objective of this review was to analyze the results from preclinical and clinical trials of these drugs for the treatment of cancer. (frontiersin.org)
  • Aslthough all these drugs have produced good results in preclinical studies of multiple cancers, the outcomes of clinical trials have not been similar. (frontiersin.org)
  • The publication describes the preclinical activity of Secarna's high-affinity bimodal LNAplus™-based ASO, IM-T9P1, that simultaneously triggers toll-like receptor 9 (TLR9) signaling and downregulates programmed cell death ligand 1 (PD-L1) expression, a protein involved in the suppression of the adaptive immune system, in one molecular entity. (izb-online.de)
  • Preclinical proof of concept for this drug class in combination with RAS/RAF, PI3K/mTOR and hypoxia-inducing targeted therapeutics. (mainlinehealth.org)
  • These data identify novel therapeutic vulnerabilities and may inform future trials of IGF inhibitory drugs. (nature.com)
  • Potent cell growth inhibitory properties were shown by ligands MS47 and MS49 against human melanoma MDA-MB-435, colon cancer HCT-116 and COLO 205, and pancreatic cancer MIA PaCa-2 cell lines, as evidenced by MTT assay. (openmedicinalchemistryjournal.com)
  • Inhibitor of checkpoint kinase CHK1 was identified as a top screen hit. (nature.com)
  • The new Immuno-checkpoint Artificial Reporter (IcAR) technology has been licensed to OncoHost , an Israeli-guided oncology company, which will seek US Food and Drug Administration approval. (timesofisrael.com)
  • This is the "checkpoint" that the cancer cells put up to defend themselves. (timesofisrael.com)
  • Using a dual luciferase assay, we assessed the effect of JMJD5 on the transcriptional activity of the c-Myc target gene. (morulaivf.com)
  • however, cytotoxicity studies on colorectal cancer cells are scarce. (bvsalud.org)
  • In this study, the cytotoxicity of 8-methoxy-2,5-dimethyl-5H-indolo[2,3-b] quinoline (MMNC) in colorectal cells was evaluated. (bvsalud.org)
  • We show that an epigenetically instilled tumor-intrinsic interferon response program confers enhanced LN metastatic potential by enabling the evasion of NK cells and promoting LN colonization. (stanford.edu)
  • This thesis work aims to examine the diverse phenotype and function of myeloid cells in primary and metastatic disease, ultimately contributing to an improved understanding of the interplay between the innate and adaptive immune system in the solid tumor microenvironment. (ubc.ca)
  • and (4) refining "precision medicine" for metastatic breast cancer using functional assays in patient-derived breast tumor grafts. (utah.edu)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Ying-Ting Lin*, "A tandem regression-outlier analysis of a ligand cellular system for key structural modifications around ligand binding" Journal of Cheminformatics, (2013), 5:21. (kmu.edu.tw)
  • Curcumin can be developed as a therapeutic drug through improvement in formulation properties or delivery systems, enabling its enhanced absorption and cellular uptake. (blogspot.com)
  • The data on the prognostic and predictive role of the tumor molecular biomarkers, as well as on clinically used and cellular therapy approaches and developing targeted drugs are presented, and the prospects for the future research are outlined. (cttjournal.com)
  • The therapy interferes with the tumor's attempts to suppress the immune system's T cells' ability to attack the cancer. (timesofisrael.com)
  • You need to know how many of the ligands can suppress the T cells. (timesofisrael.com)
  • Special advances are achieved in HER-positive tumors using targeted therapy with drugs which suppress the tumor cell growth factors (trastuzumab, herceptin). (cttjournal.com)
  • H-29 cells were used for in vitro experiments in this study. (wjgnet.com)
  • The four peptides efficiently inhibited CXCL12-dependent migration at concentrations as low as 10 nM and delayed CXCL12-mediated wound healing in PES43 human melanoma cells. (cnr.it)
  • Both ligands were more potent against cancer cells than in skin normal CCD-1064Sk fibroblasts. (openmedicinalchemistryjournal.com)
  • 2013. Brine shrimp lethality assay 'an effective prescreen': Microwave-assisted synthesis, BSL toxicity and 3DQSAR studies-based designing, docking and antitumor evaluation of potent chalcones. (cytoskeleton.com)
  • Unfortunately, some targets with potent oncogenic activity fall into this category, and currently little is known about how to solve this problem, which largely hampered drug research on human cancers. (techscience.com)
  • Billingsley ML. Druggable targets and targeted drugs: enhancing the development of new therapeutics. (jscimedcentral.com)
  • Here we introduce CODD-Pred (COmprehensive Drug Design Predictor), an online web server with well-curated data sets from the GOSTAR database, which is designed with a dual purpose of predicting potential protein drug targets and computing bioactivity values of small molecules. (bvsalud.org)
  • Subsequently, the effect of an additional small molecular inhibitor, BMS-777607 (which targets MST1R (RON), MET, Tyro3, and Axl) also resulted in a decreased proliferative capacity of MPM cells. (frontiersin.org)
  • A ligand can be referred to as an «electron donor» that is attracted to the electron acceptor at the center of a complex, for which the ligand [ion or molecule] binds to a central metal atom and forms a complex. (peptidesguide.com)
  • A key mechanism of immune evasion deployed by tumour cells is to reduce neoantigen presentation through down-regulation of the antigen presentation machinery. (portlandpress.com)
  • Their expression in tumour cells is modulated by a complex interplay of genomic, transcriptomic and post translational factors involving multiple intracellular antigen processing pathways. (portlandpress.com)
  • The discovery of MHC-II on tumour cells has been less characterized. (portlandpress.com)
  • Physical nanoscale conduit-mediated communication between tumour cells and the endothelium modulates endothelial phenotype. (cetbwh.org)
  • The recently described role of the Wnt/β-catenin pathway in the regulation of immune cell infiltration of microenvironment tumor resumes interest because of its potential impact to rection of immune therapy. (chemdiv.com)
  • 2 ] The living cell is an autonomous cybernetic system connected to, and receiving, information from its environment through an energy pathway and an information pathway. (cancer.gov)
  • These include EpiScreen™, a T-cell epitope mapping technology that screens peptides for their immunostimulatory properties, and EpiScreen DC:T cell assay, which screens biologics for their immunogenicity. (genengnews.com)
  • Typically, low molecular weight drugs are not recognized by the immune system, whereas protein molecules of much higher molecular weight invariably are. (genengnews.com)
  • This is due to the fact that certain amino acid sequences, so-called T-cell epitopes, when presented by antigen-presenting cells and recognized by helper T cells, will stimulate a powerful immune response. (genengnews.com)
  • Our primary pursuits are discovering and elucidating new molecules that regulate immune responses, investigating the biology of regulatory T cells, and better understanding the specific biochemical signatures that allow a patient's T cells to selectively target cancer cells. (hopkinsmedicine.org)
  • Her team collaborates extensively with faculty at the Bloomberg~Kimmel Institute for Cancer Immunotherapy and leads the BKI immunotherapy drug discovery core, aimed at developing new immune-targeting drug therapies for laboratory and clinical testing at Johns Hopkins. (hopkinsmedicine.org)
  • Immunotherapy is a treatment that boosts the body's immune system to help find and destroy cancer cells. (timesofisrael.com)
  • These drugs inhibit immune-suppressive signals and reinstate cancer immunosurveillance. (izb-online.de)
  • In their pioneering studies of IDO enzymes in tumoral immune escape, LIMR scientists discovered that the IDO enzyme also contributes to tumor cell survival beyond immunomodulation. (mainlinehealth.org)
  • prostate cancer cell lines and a synergistic effect between both was also o bserved. (researchgate.net)
  • First, given evidence that IGFs regulate the response to IR, we also found evidence that IGF-1R depletion induced endogenous DNA lesions marked by γH2AX foci in prostate cancer cells [ 10 ]. (nature.com)
  • The development of platinum-drug resistance in cancer patients, the general toxicity and severe side effects of platinum drugs however required a different approach in the research of anticancer metal complexes [ 2 ]. (hindawi.com)
  • Treatment options are limited at best and drug resistance is common. (frontiersin.org)
  • Although these diseases arise from the transformation of different cell types (namely melanocytes, CD4+ T cell and epithelial cells), they share common features such as a partially elucidated pathophysiology, a lack of treatment at advanced stages, a need to understand the mechanisms leading to relapse or resistance upon currently available treatments and consequently a need for alternative therapies. (hipi-lab-saint-louis.fr)
  • drug resistance, and significant systemic adverse effects. (pdfplayer.org)
  • By addressing the limitations of conventional chemotherapy, such as poor biodistribution, cancer cell drug resistance, and significant systemic adverse effects, NPs hold great promise of drastically transforming the face of oncology[1-2]. (pdfplayer.org)
  • The ligand-binding assay methodology quantified the concentration of the hormone in the test material by comparing the effects of the test sample to the results of varying amounts of known protein (ligand). (wikipedia.org)
  • Ligand binding assays provide a measure of the interactions that occur between two molecules, such as protein-bindings, as well as the degree of affinity (weak, strong, or no connection) for which the reactants bind together. (wikipedia.org)
  • The only ligand recognized to bind MST1R (RON) is Macrophage Stimulating 1 (MST1), also known as Macrophage Stimulating Protein (MSP) or Hepatocyte Growth Factor-Like Protein (HGFL). (frontiersin.org)
  • Here, I investigate the role of one intrinsic factor (receptor-type protein tyrosine phosphatase alpha) and one extrinsic factor (ionizing radiation) in facilitating cancer cell invasion. (ubc.ca)
  • Receptor-type protein tyrosine phosphatase alpha (PTPα is a widely expressed transmembrane-bound protein that has been implicated in integrin signaling, focal adhesion formation, and normal cell migration. (ubc.ca)
  • T cells have a PD1 protein "off switch" that tells the T cells to leave the other cells alone. (timesofisrael.com)
  • Protein quantitation was performed using the Pr ecision Red Protein Assay Reagent (Cat. (cytoskeleton.com)
  • An ASSAY UNIT is defined as the amount of HTS02 protein needed to achieve a tubulin polymerization signal of OD340 of 0.1 - 0.15 in 30 minutes at 37°C in G-PEM buffer. (cytoskeleton.com)
  • The protein concentration for HTS02 in this assay is approximately 4 mg/ml. (cytoskeleton.com)
  • The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. (cancerindex.org)
  • Neural factors are a class of protein molecules with neurotrophic activity that can promote the survival and regeneration of nerve cells. (acrobiosystems.com)
  • Thus temporally increased cell permeability enables extracellular molecules with otherwise hampered transmembrane transport to enter the cells. (hindawi.com)
  • This seemed much larger than would be expected for the range of space occupied by drug-like chemical compounds, estimated at 300 to 1000 Å2 [4, 5]. (cancer.gov)
  • Therefore, a rationale for treating hypertension would be to administer drugs or natural compounds which selectively inhibit ACE. (scielo.br)
  • Antineoplastons are drugs composed of chemical compounds that are naturally present in the urine and blood . (cancer.gov)
  • Such peptides are believed to be "promiscuous" T-cell epitopes that bind to the HLA supertypes on the surface of antigen-presenting cells that are broadly represented in the world's population. (genengnews.com)
  • All three peptides significantly inhibited subcutaneous growth of SN12C-EGFP renal cancer cells. (cnr.it)
  • Developing new techniques for screening and synthesizing peptides can result in efficient and cost-effective methods which make drugs more effective, diseases easier to cure, and lives to last longer. (peptidesguide.com)
  • Here huge numbers of peptides are screened so as to isolate and identify critical bioactive peptides that are used for new ways to cure diseases and cancers. (peptidesguide.com)
  • Peptides that display the desired activity are identified by zones of reduced cell growth, or by other indicators such as color changes. (peptidesguide.com)
  • This method identifies peptide ligands as well as cytoxic peptides that may prove useful in developing anti-cancer agents. (peptidesguide.com)
  • If ER stress is a significant factor that affects the disease development, it would be important to find a drug that alters these mechanisms and UPR. (helsinki.fi)
  • Because of their passive and ligand- based targeting mechanisms, nanoparticles (NPs) accumulate preferentially at tumor sites.The recent utilization of nanotechnology in CC diagnosis and therapy and the development of HPV vaccinations. (pdfplayer.org)
  • A detection method is used to determine the presence and extent of the ligand-receptor complexes formed, and this is usually determined electrochemically or through a fluorescence detection method. (wikipedia.org)
  • Detection of single mRNAs in individual cells of the auditory system. (omeka.net)
  • Hence, blood biomarker detection has become an efficient screening tool for the early diagnosis of OSCC. (techscience.com)
  • Analyzing the pivotal trial that compared sunitinib and IFN-a in renal cell carcinoma, using a method that assesses tumor regression and growth. (jscimedcentral.com)
  • In Chapter 4, I profiled the dynamic myeloid compartment following antigen specific CD8⁺ T cell-mediated tumor regression and recurrence. (ubc.ca)
  • I identified several potentially suppressive myeloid cell subsets, including TAMs and arginase-1ʰⁱ monocytic-MDSCs (M-MDSCs) that expand during CD8⁺ T cell-mediated tumor regression and subsequent recurrence, which may play a role in tumor regrowth. (ubc.ca)
  • Jin-Ching Lee, Chin-kai Tseng, Kuan-Jen Chen, Kuo-Jung Huang, Chun-Kuang Lin, Ying-Ting Lin*, "A Cell-based Reporter Assay for inhibitor screening of Hepatitis C Virus RNA dependant RNA Polymerase. (kmu.edu.tw)
  • As a direct result of these monumental findings, researchers have continued the advancement of ligand binding assays in many facets in the fields of biology, chemistry, and the like. (wikipedia.org)
  • Low-affinity CAR T cells exhibit reduced trogocytosis, preventing rapid antigen loss, and increasing CAR T cell expansion. (utah.edu)
  • Their current application in oncology has revolutionized cancer treatment by improving the antitumor activity of several chemotherapeutic agents. (mdpi.com)
  • Nanoparticles (NPs) are tailored drug delivery vectors that can selectively target huge dosages of chemotherapeutic drugs or therapeutic genes into cancer cells while leaving healthy cells alone. (pdfplayer.org)
  • Historically, ligand binding assay techniques were used extensively to quantify hormone or hormone receptor concentrations in plasma or in tissue. (wikipedia.org)
  • Our previous work allowed the identification of the first positive marker for MF and SS cutaneous and circulating malignant T cells, namely the NK cell receptor KIR3DL2. (hipi-lab-saint-louis.fr)
  • Estrogen receptor alpha mutations regulate gene expression and cell growth in breast cancer through microRNAs. (utah.edu)
  • Abstract Oral squamous cell carcinoma (OSCC) is a malignant tumor triggered by the accumulation of multiple gene mutations in oral epithelial cells. (techscience.com)
  • Other types include squamous cell carcinoma (see the image below) and adenocarcinomas. (medscape.com)
  • Cross-section through the bladder, uterus, and vagina with squamous cell carcinoma of the bladder infiltrating through the bladder wall into the vaginal wall. (medscape.com)
  • Squamous cell carcinoma (SCC) is the second most common cell type associated with bladder cancer in industrialized countries. (medscape.com)
  • The main challenges left to be solved is the evaluation of restraint goodness, assigning secondary structures, restraint interconditioning, and water and ligand placement. (helsinki.fi)
  • Our team has expertise in skin cancers, with a special interest in cutaneous T cell lymphoma, melanoma and carcinoma. (hipi-lab-saint-louis.fr)
  • Our team develops research projects dedicated to skin cancers with a main interest on melanoma, cutaneous T cell lymphoma (CTCL) and carcinoma. (hipi-lab-saint-louis.fr)
  • The role of the medicinal chemist in drug discovery--then and now. (jscimedcentral.com)
  • EFMC would like to remind his efforts, joint with Henk Timmerman and Han van de Waterbeemd, to launch the successful series of EFMC Short Course in Medicinal Chemistry in 2009 with a very successful course on "Improving Compound Quality: Physical Chemistry and DMPK Properties in Drug Discovery. (efmc.info)
  • Terry Rabbitts is Professor of Molecular Immunology in the Centre for Cancer Drug Discovery, Institute of Cancer Research, Sutton, London, UK. (cancer.gov)
  • Mechanism-based target identification and drug discovery in cancer research. (jscimedcentral.com)
  • Organic chemistry in drug discovery. (jscimedcentral.com)
  • Target identification and bioactivity prediction are critical steps in the drug discovery process. (bvsalud.org)
  • TL238) for anti-tubulin ligand drug discovery. (cytoskeleton.com)
  • Explore our series of recombinant neural factors to support the culture and differentiation of nerve cells. (acrobiosystems.com)
  • We mainly focus our research activity on two types of CTCL: the Mycosis Fungoides (MF), where tumor cells accumulate in the skin, and the Sézary syndrome (SS), a highly aggressive form characterized by the presence of malignant cells in the skin and blood. (hipi-lab-saint-louis.fr)
  • We recently reported that CD39, an ectonuclotidase involved in the process of generating adenosine from ATP, is aberrantly expressed by Sézary patients' malignant cells. (hipi-lab-saint-louis.fr)
  • Traditional chemotherapy is nonspecific in its targeting of malignant cells, leaving normal healthy cells vulnerable to the drug's side effects. (pdfplayer.org)
  • Relative cell viability was assessed using MTT assays. (wjgnet.com)
  • We describe antitumor activities of vitamin E succinate (VES), an anti-oxidant and fenbendazole (FBZ), a commonly used veterinary anthelmintic. (researchgate.net)
  • Epidemiological and clinical studies have suggested that cancer could be prevented or significantly reduced by treatment with anti-oxidant and anti-inflammatory drugs, therefore, curcumin, a principal component of turmeric (a curry spice) showing strong anti-oxidant and anti-inflammatory activities, might be a potential candidate for the prevention and/or treatment of cancer and other chronic diseases. (blogspot.com)
  • Anticancer drugs: Keeping one step ahead. (jscimedcentral.com)
  • PN has been touted as a promising carrier for anticancer drugs among targeted drug delivery systems.We reviewed numerous ways for directing nanoparticles to a specific site and preparation processes, patent inventions, and prospects in this study. (pdfplayer.org)
  • In addition, rhodomycin A rendered gefitinib-resistant lung adenocarcinoma cells more sensitive to gefitinib treatment, implying a synergistic effect of the combination therapy. (oncotarget.com)
  • Antiangiogenic agents as second-line therapy for advanced non-small cell lung cancer. (jscimedcentral.com)
  • Li X, Taratula O, Taratula O, Schumann C, Minko T. LHRH-Targeted Drug Delivery Systems for Cancer Therapy. (jscimedcentral.com)
  • The effectiveness or lack of a major therapeutic effect of sphingolipid modulation by some drugs as a cancer therapy and other aspects related to their mechanism of action are discussed in this review. (frontiersin.org)
  • For the developer, cell differentiation is the key to cancer therapy. (cancer.gov)
  • The notion of controlling tumor growth through a naturally occurring biochemical mechanism in the body that directs cancer cells into normal channels of differentiation is one of the theoretical foundations of antineoplaston therapy. (cancer.gov)
  • for example, hydrophilic surfaces give NPs stealthy properties for extended circulation durations, while positively charged surfaces can help cancer cells internalize more rapidly[1].Dendrimers, for example, are a kind of NP now being studied for cancer therapeutic purposes.This article reviews the targeting features of cancer therapy as well as numerous polymer-based nanocarriers. (pdfplayer.org)
  • BM-derived DC (BMDC) are powerful antigen-presenting cells. (stanford.edu)
  • Some newer types are called "mix-and-measure" assays because they do not require separation of bound from unbound ligand. (wikipedia.org)
  • During normal cell migration, cells use focal adhesions to facilitate cycles of cell adhesion to, and release from, the extracellular matrix (ECM). (ubc.ca)
  • Finally, we showed that ssDNA lesions are converted to toxic DSBs in cells lacking functional ataxia telangiectasia mutated (ATM), likely due to failure to form 53BP1 bodies and/or a role for ATM in SSB repair or fork protection [ 14 ]. (nature.com)
  • By comparing responses to different amino acid sequences, a T-cell epitope map can be built up which allows the scientist to predict what modification can be designed into the Factor VIII molecule to make it less immunogenic. (genengnews.com)
  • There are two common strategies that are adopted for this type of experiment: Increasing the amount of radioligand added while maintaining both the constant specific activity and constant concentration of radioligand, or decreasing the specific activity of the radioligand due to the addition of an unlabeled ligand. (wikipedia.org)
  • His achievements in understanding the role of physiochemical properties in drug activity, more precisely in the disposition of medicines ought to be particularly recognised. (efmc.info)
  • Confirming its role in enhancing c-MYC activity, we found that elevated OTUB1 correlates with inferior clinical outcomes in the c-MYC-dependent cancer multiple myeloma, and overexpression of OTUB1 accelerates the growth of myeloma cells. (morulaivf.com)
  • The combined downregulation of PD-L1 expression in a single-molecule approach resulted in exceptional antitumor activity in different tumor models. (izb-online.de)
  • The activity of IM-T9P1 was tested in cancer cell lines and tumor models. (izb-online.de)
  • Libraries in and of themselves can prove even more uniform than other methods in demonstrating full-length ligands during screening since molecular weight degeneracy can be eliminated right during the sequencing process versus during the synthesis, thus making the entirety of the technique much more simplified and less costly. (peptidesguide.com)