AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceNamed GroupsHealth Care
GranulocytesGranulocyte Colony-Stimulating FactorReceptors, Granulocyte Colony-Stimulating FactorGranulocyte-Macrophage Colony-Stimulating FactorNeutrophilsLeukocytesHematopoietic Stem CellsLeukocyte CountColony-Stimulating FactorsHematopoiesisAgranulocytosisBone Marrow CellsNeutropeniaRecombinant ProteinsBone MarrowHematopoietic Stem Cell MobilizationInterleukin-3Colony-Forming Units AssayPhagocytosisMonocytesCell DifferentiationCells, CulturedLeukemia, MyeloidRespiratory BurstAntigens, CD34Leukocyte TransfusionN-Formylmethionine Leucyl-PhenylalanineMacrophagesEosinophilsChemotaxis, LeukocyteAntigens, CDLeukocytosisMyelopoiesisBlood CellsMice, Inbred C57BLPeroxidaseStem Cell FactorMyeloid Progenitor CellsBlood Bactericidal ActivityCytokinesGranulocyte Precursor CellsLymphocytesIndiumLeukopoiesisReceptors, Granulocyte-Macrophage Colony-Stimulating FactorNitroblue TetrazoliumGrowth SubstancesCell DivisionCell SeparationCyclophosphamideNeutrophil ActivationTime FactorsLeukopeniaHematopoietic Cell Growth FactorsLeukemia, Myeloid, AcuteZymosanHL-60 CellsHematopoietic Stem Cell TransplantationCell LineMacrophage Colony-Stimulating FactorAntibodies, MonoclonalChemokine CXCL6Myeloid CellsBlood Component RemovalRNA, MessengerLactoferrinGranulomatous Disease, ChronicMice, Inbred BALB CBone Marrow TransplantationHemoglobinuria, ParoxysmalSpleenMacrophage-1 AntigenAntineoplastic Combined Chemotherapy ProtocolsChemotactic FactorsAnemia, AplasticSuperoxidesAntigens, CD15Cell AdhesionInterleukin-6LeukemiaDose-Response Relationship, DrugCell LineageGranulocyte-Macrophage Progenitor CellsLeukotriene B4Platelet CountTetradecanoylphorbol AcetateCytapheresisCell CountOpsonin ProteinsLuminescent MeasurementsReceptors, Colony-Stimulating FactorDrug SynergismTumor Necrosis Factor-alphaInflammationCytoplasmic GranulesT-LymphocytesKineticsTransplantation, AutologousLeukemoid ReactionComplement C5a
Colony-stimulMonocytesMacrophageNeutrophilsMacrophagesNeutrophilLymphocyteDendritic cellsDifferentiationMature granulocytesVitroStimulates the productionHematopoietic progenitor cellsPeripheralGiven to the donorEosinophilsProgenitorImmature cellsWhite blood cell differPopulationsTumorFlow cytometryRecombinantMobilizationBasophilsDonorInfectionsSubstancesCytokineConditioning regimensAbnormalGranulocyticAllergicMononuclear cellsInfusionPatientsTypeInflammationCountsPlasmaInfectionReceptorTransientAntibodiesLeukemiaClustersHumanScatterPhagocyticInflammatoryMyeloid cellPromyelocytesBlastsCellularCD16SignificantlyBone Marrow Tra
Colony-stimul29
  • Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. (wikipedia.org)
  • Mouse granulocyte-colony stimulating factor (G-CSF) was first recognised and purified in Walter and Eliza Hall Institute, Australia in 1983, and the human form was cloned by groups from Japan and Germany/United States in 1986. (wikipedia.org)
  • The cytokine granulocyte-colony stimulating factor (G-CSF) is known to have trophic and neuroprotective properties in the brain, and we recently identified it as a modulator of neuronal and behavioral plasticity. (jneurosci.org)
  • Here, we report that granulocyte-colony stimulating factor (G-CSF), a pleiotropic cytokine with known trophic and neuroprotective properties in the brain, acts directly on dopaminergic circuits to enhance their function. (jneurosci.org)
  • Mice deficient in granulocyte-macrophage colony stimulating factor (GM-CSF) develop pulmonary alveolar proteinosis (PAP). (wiley.com)
  • Intradermal transgenic expression of granulocyte-macrophage colony-stimulating factor induces neutrophilia, epidermal hyperplasia, Langerhans' cell/macrophage accumulation, and dermal fibrosis. (mcmaster.ca)
  • These substances include, for instance, granulocyte- colony-stimulating factor (G-CSF), which is added so that the donors own bone marrow will make and produce more stem cells that will end up in the bloodstream. (differencebetween.net)
  • The advantage is that granulocyte- colony-stimulating factor (G-CSF) does not have to be given to the donor since the procedure involves going to the source of the stem cells, which is the bone marrow. (differencebetween.net)
  • In the case of a stem cell transplant, granulocyte- colony-stimulating factor has to be given to the donor a few days before the procedure. (differencebetween.net)
  • An autopsy case of carcinosarcoma of the liver producing granulocyte-colony stimulating factor (G-CSF) is reported. (nih.gov)
  • The patient received granulocyte-colony-stimulating factor (G-CSF) and plerixafor for HPC mobilization and underwent 3 days of HPC-A collection. (nih.gov)
  • Possible approaches include low or high doses of cyclophosphamide (Cy), other chemotherapeutic agents, or granulocyte colony-stimulating factor (G-CSF) alone. (univpm.it)
  • Human granulocyte colony-stimulating factor (G-CSF) is a regulatory glycoprotein that stimulates the production of neutrophilic granulocytes from committed hematopoietic progenitor cells both in vitro and in vivo. (ashpublications.org)
  • Decreased specific binding of 125l-G-CSF by human neutrophils was consistently observed in the presence of excess unlabeled human granulocyte-macrophage colony-stimulating factor (GM-CSF), suggesting competition or down modulation by GM-CSF of the G- CSF receptor. (ashpublications.org)
  • Propagation of dendritic cell progenitors from normal mouse liver using granulocyte/macrophage colony-stimulating factor and their maturational development in the presence of type-1 collagen. (silverchair.com)
  • Within 1 wk of liquid culture in granulocyte/macrophage colony-stimulating factor (GM-CSF), normal B10 BR (H-2k I-E+) mouse liver nonparenchymal cells (NPC) formed loosely adherent myeloid cell clusters that have been shown to contain dendritic cell (DC) progenitors in similar studies of mouse blood or bone marrow. (silverchair.com)
  • Moreover, although interleukin 7 (IL-7) supports the generation of such myeloid intermediates, we show that their developmental branching from the main intrathymic T-cell pathway is linked to the up-regulation of the myelomonocytic granulocyte macrophage-colony-stimulating factor (GM-CSF) receptor, to the down-regulation of the IL-7 receptor and to the lack of pre-T-cell receptor α (pTα) gene transcriptional activation. (ashpublications.org)
  • We isolated total CD34 + cells, CD34 + ,CD33 + cells, and CD34 + ,CD33 - cells individually from normal human bone marrow cells by fluorescence-activated cell sorter (FACS), and examined the effects of granulocyte colony-stimulating factor (G-CSF), interleukin-3 (IL-3), and IL-5 on in vitro colony formation of these cells. (elsevierpure.com)
  • Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a potent hematopoietic growth factor that both enhances the survival and drives the differentiation and proliferation of myeloid lineage cells. (elsevierpure.com)
  • This assay has high sensitivity and excellent specificity for detection of Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF). (uscnk.com)
  • No significant cross-reactivity or interference between Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) and analogues was observed. (uscnk.com)
  • Matrices listed below were spiked with certain level of recombinant Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) and the recovery rates were calculated by comparing the measured value to the expected amount of Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) in samples. (uscnk.com)
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) were tested 20 times on one plate, respectively. (uscnk.com)
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) were tested on 3 different plates, 8 replicates in each plate. (uscnk.com)
  • The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) and their serial dilutions. (uscnk.com)
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF). (uscnk.com)
  • Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF). (uscnk.com)
  • Talimogene laherparepvec is a genetically modified herpes simplex virus type 1 that is designed to replicate within tumors and produce the immunostimulatory protein granulocyte-macrophage colony-stimulating factor (GM-CSF). (medscape.com)
  • The donor had scheduled a trip to Sri Lanka, and was to return 3 days before the scheduled start of granulocyte-colony-stimulating factor, or G-CSF, application. (cdc.gov)
Monocytes7
Macrophage6
  • Resistance of the body to infection: I. leukocytes, granulocytes, the monocyte-macrophage system, and inflammation. (medlineplus.gov)
  • However, the intensity of expression of the DC-restricted markers NLDC 145, 33D1, and N418, and the macrophage marker F4/80, intercellular adhesion molecule 1, and Fc gamma RII was low to moderate, whereas the cells were negative for CD3, CD45RA, and NK1.1. (silverchair.com)
  • In vivo incorporation into human bone marrow was assayed by differential chromatid staining as well as by comparison of in vitro radiation survival curves of granulocyte-macrophage progenitor cells scored at both day 7 and day 14. (tmu.edu.tw)
  • Both CD34 + ,CD33 + cells and CD34 + ,CD33 - cells formed granulocyte/macrophage colonies in the presence of IL-3. (elsevierpure.com)
  • Although macrophage activation syndrome (MAS) is often termed autoinflammatory in both loss and gain of function phenotypes, adaptive lineage cells, especially T cells, are clearly important in both settings. (nature.com)
  • The MVX-ONCO-2 consists of two components: 1) biocompatible capsules, loaded with a genetically modified cell-line that secretes a strong immune-booster GM-CSF (granulocyte-macrophage stimulating factor) and 2) irradiated autologous patients' tumor cells, isolated from patients which serve as tumor antigens. (b3cnewswire.com)
Neutrophils9
  • The specific types of granulocytes are neutrophils, eosinophils, and basophils. (medlineplus.gov)
  • Granulocytes, specifically neutrophils, help the body fight bacterial infections. (medlineplus.gov)
  • 1 Granulocytes are the most abundant myeloid cell in the blood, and can further be divided into eosinophils , basophils , and neutrophils. (beckman.com)
  • Human neutrophils are the most abundant cell form inside of the granulocyte relatives. (flapinhibitor.com)
  • Neutrophils are detected as Ly6G optimistic cells, whereas eosinophils are recognized by their expression of CCR3 and Siglec-F. Basophils also demonstrate positivity for CD11b, but have only a reduced expression of Ly6C. (flapinhibitor.com)
  • obtainable in PMC 2022 June 03.Cossarizza et al.Page7.4 LIVE/DEAD evaluation of granulocytes-Especially within the context of learning inflammatory infiltrates, it really is often needed to establish irrespective of whether neutrophils are viable. (flapinhibitor.com)
  • G-CSF also modulates multiple differentiated functions of human neutrophils, including enhanced oxidative metabolism in response to f- Met-Leu-Phe (f-MLP), increased antibody-dependent cell-mediated cytotoxicity (ADCC), and augmented arachidonic acid release in response to ionophore and chemotactic agents. (ashpublications.org)
  • These effects are all maximal at a concentration of 100 to 500 pmol/L. Using 125I-labeled recombinant human G-CSF, high affinity binding sites were identified on human neutrophils, the myeloid leukemia cell lines KG-1 and HL-60, and the small cell carcinoma cell lines, H128 and H69. (ashpublications.org)
  • [ 10 , 12 ] G-CSF suppresses apoptosis and prolongs the survival of neutrophils in vivo in a CD34 + cell population. (medscape.com)
Macrophages8
  • G-CSF is produced by endothelium, macrophages, and a number of other immune cells. (wikipedia.org)
  • Granulocytes, macrophages, and dendritic cells arise from a common maj" by Kayo Inaba, Muneo M. Inaba et al. (rockefeller.edu)
  • The distinct MAS phenotype that arises with hypercytokinaemia can be explained by activation of macrophages that are closely juxtaposed to sinusoids that permit direct phagocytic access to haematopoietic lineage cells. (nature.com)
  • Macrophages, which are functional plasticity cells, have the ability to phagocytize and digest foreign substances and acquire pro-(M1-like) or anti-inflammatory (M2-like) phenotypes according to their microenvironment. (frontiersin.org)
  • The large number of macrophages in the intestinal tract, play a significant role in maintaining the homeostasis of microorganisms on the surface of the intestinal mucosa and in the continuous renewal of intestinal epithelial cells. (frontiersin.org)
  • In addition, the effects of macrophages in the occurrence and development of inflammatory bowel disease (IBD), and their role in inducing fibrosis, activating T cells, reducing colitis, and treating intestinal inflammation were also reviewed in this paper. (frontiersin.org)
  • On the other hand, phagosomes are formed when the pathogen is engulfed by macrophages and fuse with lysosomes to release enzymes and toxic substances, resulting in killing or having cytotoxic effects on bacteria and tumor cells. (frontiersin.org)
  • The lamina propria of the small intestine is the main site of the intestinal immune system, which contains a large number of macrophages, CD4 T cells, and dendritic cells. (frontiersin.org)
Neutrophil6
  • Further benefits are that the platelets and neutrophil cells (one of the white blood cells) are more easily able to recover if this method is used compared with the bone marrow procedure. (differencebetween.net)
  • The receptor usage of the CXC chemokine granulocyte chemotactic protein-2 (GCP-2) was compared with that of interleukin-8 (IL-8) and epithelial-cell-derived neutrophil attractant-78 (ENA-78). (tau.ac.il)
  • It was concluded that G-CSF supported the neutrophil differentiation of committed colony-forming cells, IL-3 supported that of both committed and multipotent colony-forming cells. (elsevierpure.com)
  • Granulocytes are typically transfused daily for 5 or more consecutive days and should be continued until infection resolves or absolute neutrophil count remains >500/uL for 48 hours. (medscape.com)
  • Congenital neutrophil defects (i.e., chronic granulomatous disease) and documented refractory bacterial or fungal infection failing to respond to appropriate antimicrobial therapy for more than 24 to 48 hours may be considered for granulocyte transfusion. (medscape.com)
  • Differential blood count is also used along with leukocyte count (WBC) to generate an absolute value for each type of white blood cells (eg, absolute neutrophil count, absolute lymphocyte count, or absolute eosinophil count), which usually gives more meaningful information than the percentage of each, since relative percentage can be misleading. (medscape.com)
Lymphocyte3
  • This episode paralleled with a massive CD8 T- lymphocyte expansion (CD3-663, CD4 106, CD8-568 cells/microliter) following granulocyte infusions, which peaked at day +10 post HSCT. (ebmt.org)
  • This range of highly validated ELISA kits from Hycult can be used to measure granulocyte and lymphocyte proteins in a wide variety of samples. (bioscience.co.uk)
  • Leukocyte adhesion deficiency results from an adhesion molecule defect that causes granulocyte and lymphocyte dysfunction and recurrent soft-tissue infections. (msdmanuals.com)
Dendritic cells3
  • Dendritic cells are antigen-presenting cells that act as the link between innate and adaptive immunity. (beckman.com)
  • In this study, the finding that a significant proportion of all dendritic cells (DCs) resident in vivo in the human postnatal thymus displayed a myeloid-related phenotype prompted us to re-examine the developmental origin of thymic DCs, a cell type hitherto considered to represent a homogeneous lymphoid-derived population. (ashpublications.org)
  • Dendritic cells (DCs) are hematopoietic-derived highly specialized antigen-presenting cells (APCs) that display potent ability to induce both specific immune responses and deletion of potentially autoreactive T cells. (ashpublications.org)
Differentiation4
  • White blood cells The G-CSF-receptor is present on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates proliferation and differentiation into mature granulocytes. (wikipedia.org)
  • We show here that these novel intrathymic DCs are truly myeloid, as they arise from CD34 + early thymic progenitors through CD34 lo intermediates which have lost the capacity to generate T cells, but display myelomonocytic differentiation potential. (ashpublications.org)
  • M, Peterson C. 2018 Haematopoietic stem suggests that during differentiation a stem cell approaches a stable equili- cells: entropic landscapes of differentiation. (lu.se)
  • Single cells in these populations exhibit different com- stem cell differentiation, Shannon information binations of regulator activity that suggest the presence of multiple theory, entropy configurations of a potential differentiation network as a result of multiple entry points into the committed state. (lu.se)
Mature granulocytes1
  • Our results indicated that CD10-positive granulocytes were significantly decreased in BM of MDS patients than AA and IDA patients, and the level of CD10-positive mature granulocytes was not associated with the clinical stages of malignancy. (techscience.com)
Vitro4
  • The recombinant peptide corresponding to residues 301-376 at the junction of constant regions 2 and 3 of the human IgE ε chain blocked the in vivo passive sensitization of human skin mast cells and in vitro sensitization of human basophil granulocytes with human IgE antibodies. (johnshopkins.edu)
  • G-CSF receptor numbers ranged between 138 and 285 sites per cell with a kd of 77 to 140 pmol/L, consistent with the concentrations of G-CSF that elicit biologic responses in vitro. (ashpublications.org)
  • Although a difference was found in the radiation survival of control (untreated) day-7 progenitor cells (Do = 1.39 Gy) and day-14 progenitor cells (Do = 0.89 Gy), a similar degree of in vitro radiosensitization was found for BUdR-treated bone marrow progenitor cells scored at day 7 and day 14. (tmu.edu.tw)
  • To understand its neuroprotective effect in vitro, GM-CSF was administered to a glutamate-induced excitotoxicity neuronal injury cell culture model that mimics the pathophysiology of focal hypoxic cerebral injury. (elsevierpure.com)
Stimulates the production1
Hematopoietic progenitor cells1
  • Collection of hematopoietic progenitor cells by apheresis (HPC-A) requires separation of cells by density. (nih.gov)
Peripheral5
  • In order to assess the incidence and analyze reasons which cause prolongation of hospital stay in patients engrafted after peripheral blood stem cell transplantation (PBSCT), we performed this retrospective analysis. (nature.com)
  • Peripheral blood stem cell and bone marrow transplantation for solid tumors and lymphomas: hematologic recovery and costs. (nature.com)
  • Total of 107 EDTA anticoagulated peripheral blood samples were randomly selected from daily pools of samples irrespective of the age, gender, or clinical condition samples, with wide ranges of immature granulocytes counts. (horiba.com)
  • Here, we report transmission of dengue virus to a peripheral blood stem cell recipient by a donor who had recently traveled to an area to which the virus is endemic. (cdc.gov)
  • Differential blood count gives relative percentage of each type of white blood cell and also helps reveal abnormal white blood cell populations (eg, blasts, immature granulocytes, or circulating lymphoma cells in the peripheral blood). (medscape.com)
Given to the donor1
  • The amount of granulocytes per dose can vary greatly (from 1-8 x 1010 granulocytes/collection) based on the donor and mobilization regimen given to the donor. (medscape.com)
Eosinophils1
  • Eosinophils are a type of granulocyte that play a crucial role in innate and adaptive immunity. (beckman.com)
Progenitor1
  • 8 As these latter DCs derive from an intrathymic lymphoid-restricted progenitor able to generate also T, B, and NK cells, but not myeloid cells, upon transfer in vivo, 5 , 9 , 10 they have hitherto been considered of lymphoid origin. (ashpublications.org)
Immature cells2
  • High Side Scatter is measured for granulocytes, and fluorescence is higher for immature cells having more residual RNA. (horiba.com)
  • Immature granulocytes (IGs) encompass immature cells of granulocytic lineages, including metamyelocytes, myelocytes, and promyelocytes, which are easily recognized morphologically and are reported by automated analyzer as IG altogether. (medscape.com)
White blood cell differ2
  • Granulocytes are counted as part of a white blood cell differential test . (medlineplus.gov)
  • In the Sysmex XN-3000 analyzer, the IGs are measured in the white blood cell differential (WDF) channel where the cells are labeled with a dye binding to nucleic acids (Fluorocell WDF), then pass the beam of a semiconductor laser. (horiba.com)
Populations2
  • 1-3 These nonoverlapping functions have been proposed to result from the actions of 2 major DC populations which have been characterized as myeloid and lymphoid DCs, respectively, on the basis of their anatomical localization and cell-surface phenotypes and, ultimately, of their distinct developmental origin. (ashpublications.org)
  • Fibrotic livers are characterized by an inflammatory microenvironment that is composed of various immunologically active cells, including liver-resident populations (e.g. (mdpi.com)
Tumor3
  • Unlike the splenic DC, however, most of the GM-CSF-propagated putative liver DC harvested at 6-10 d expressed only a low level of major histocompatibility complex (MHC) class II (I-Ek), and they failed to induce primary allogeneic responses in naive T cells, even when propagated additionally in GM-CSF and tumor necrosis alpha and/or interferon gamma-supplemented medium. (silverchair.com)
  • To induce an efficient anti-tumor response against the patients' own tumor cells, the encapsulated GM-CSF secreting cells are implanted subcutaneously at the site of tumor-cell vaccination. (b3cnewswire.com)
  • The autologous patients' tumor cells will be processed at the clinical sites. (b3cnewswire.com)
Flow cytometry2
Recombinant2
  • When the mononuclear cells of two normal individuals were preincubated with the recombinant peptide or E myeloma protein for 15 min before passive sensitization with the same allergic serum and the cells were challenged with an optimal concentration of an antigen, ~ 11- to 13-fold higher concentration of the recombinant peptide than E myeloma protein was required for 50% inhibition of antigen-induced histamine release. (johnshopkins.edu)
  • In this report, we show that biosynthetic (recombinant) human G-CSF enhances colony formation by normal human bone marrow and the human myeloid leukemic cell lines, HL-60 and KG-1, as well as nonhematopoietic small cell lung cancer lines, H128 and H69. (ashpublications.org)
Mobilization3
  • Hematopoietic System G-CSF is also a potent inducer of hematopoietic stem cell (HSC) mobilization from the bone marrow into the bloodstream, although it has been shown that it does not directly affect the hematopoietic progenitors that are mobilized. (wikipedia.org)
  • The optimal stem cell (SC) mobilization strategy for patients with multiple myeloma (MM) remains a matter of debate. (univpm.it)
  • The stem cell mobilization result was poor. (cdc.gov)
Basophils1
Donor6
  • T-replete mismatched cord blood transplant (TRCB) offers an augmented graft versus leukemia (GVL) effect mediated by alloreactive donor T cells and thereby presents a potential for long term remission and cure in high risk and relapsed refractory AML in children. (ebmt.org)
  • G-CSF is also used to increase the number of hematopoietic stem cells in the blood of the donor before collection by leukapheresis for use in hematopoietic stem cell transplantation. (wikipedia.org)
  • This is when stem cells that are obtained from the bloodstream or umbilical cord of a donor are used for transplant into a patient. (differencebetween.net)
  • The donor first has to be treated with G-CSF, and then the stem cells can only be harvested a couple of days after the donor since time is needed for the donor's body to form more stem cells and transport these cells into the bloodstream. (differencebetween.net)
  • The volume will vary based on granulocyte concentration of donor but usually ranges from 10-20 mL/kg. (medscape.com)
  • Often times granulocyte donors are friends and family of the recipient because of the commitment needed to be a granulocyte donor. (medscape.com)
Infections9
  • Chemotherapy can cause myelosuppression and unacceptably low levels of white blood cells (leukopenia), making patients susceptible to infections and sepsis. (wikipedia.org)
  • People with a lower number of granulocytes are more likely to develop serious infections. (medlineplus.gov)
  • Immature Granulocyte count (IMG) is used in the assessment of various infections or sepsis and its severity 1,2 , inflammatory conditions like Pancreatis 3 , Appendicitis 4 , Acute Respiratory Distress Syndrome (ARDS) 5 , different diseases of bone marrow, transplant engraftment or growth factor therapy or hematopoietic neoplasms. (horiba.com)
  • Measurement of immature granulocyte percentage (%IG) and Platelet Larger Cell Ratio (PLCR) may be used as a marker of serious bacterial infections. (jsurgmed.com)
  • Lymph contains white blood cells , which help the body fight infections. (medicalnewstoday.com)
  • This can include infections and cancer cells. (medicalnewstoday.com)
  • Lymph nodes also contain immune cells that help fight infections. (medicalnewstoday.com)
  • Phagocytic cell defects Immunodeficiency disorders are associated with or predispose patients to various complications, including infections, autoimmune disorders, and lymphomas and other cancers. (msdmanuals.com)
  • Granulocyte transfusion is a supportive adjuvant therapy to manage infections uncontrolled by standard medical therapy and thus should not be used as first line treatment or as the sole treatment of infection. (medscape.com)
Substances1
  • Before cells can be extracted from the blood various substances have to be introduced, to help trigger the growth of the stem cells. (differencebetween.net)
Cytokine4
  • D) Cytokine release syndrome following granulocyte infusion and consider anti IL-6 antibody or steroids if further deterioration. (ebmt.org)
  • The reverse haemolytic plaque assay allows the measurement of cytokine production at a single cell level. (ersjournals.com)
  • The current authors have previously demonstrated, using a reverse haemolytic plaque assay (RHPA), that single-cell production of cytokine can be assayed 16 . (ersjournals.com)
  • This technique has an advantage over routine culture conditions, as the development of cytokine product by individual cells can be localised and measured, which therefore allows for the correlation of cytokine production with cell type. (ersjournals.com)
Conditioning regimens1
Abnormal2
  • Previous studies highlighted the challenges of HPC-A collection from patients with abnormal red blood cells (RBCs). (nih.gov)
  • Babu E, Basu D. Platelet large cell ratio in the differential diagnosis of abnormal platelet counts. (jsurgmed.com)
Granulocytic1
  • Human granulocytic anaplasmosis (HGA), formerly known as human granulocytic ehrlichiosis (HGE), is caused by Anaplasma phagocytophilum, which infect granulocytes. (medscape.com)
Allergic1
  • Fevers, chills, and allergic reactions are not uncommon with granulocyte transfusions. (medscape.com)
Mononuclear cells2
  • Pathologically, at an early stage, there is an influx of mononuclear cells followed by lymphocytes. (ersjournals.com)
  • However, it is not clear whether TNF production in disease sites is a feature of all mononuclear cells that traffic there or whether it is a subset phenomenon. (ersjournals.com)
Infusion1
Patients6
  • 125 I-GM-CSF binding to TF-1 cells was prevented in the presence of BALF from IPAP patients. (wiley.com)
  • As a surface marker of granulocyte, studies have shown CD10 can be used to define the degree of granulocyte maturation in MDS patients. (techscience.com)
  • Receiver operating characteristic (ROC) areas under the curve (AUC) of CD10-positive granulocytes was 0.86 and 0.85, respectively, in MDS patients than the IDA group and AA group with good specificity and sensitivity. (techscience.com)
  • Patients with TEMPI syndrome have responded to therapies used to treat plasma cell dyscrasias and may benefit from autologous HPC transplantation. (nih.gov)
  • Les concentrations sériques de sCD40L circulant et d'interleukine 10 circulante ont été analysées à l'aide de la méthode immuno-enzymatique chez 30 patients positifs pour le VHC avec un CHC, chez 30 patients patients positifs pour le VHC avec une cirrhose du foie, et chez 30 volontaires d'âge correspondant en bonne santé avec des anticorps anti-VHC négatifs servant de groupe témoin. (who.int)
  • Hematopoietic stem cell transplantation has become a major treatment option for patients with hematopoietic malignancies and immune deficiencies. (cdc.gov)
Type6
  • Granulocytes are a type of white blood cell that has small granules inside them. (medlineplus.gov)
  • However, when 7-d cultured GM-CSF-stimulated liver cells were maintained additionally for three or more days on type-1 collagen-coated plates in the continued presence of GM-CSF, they exhibited characteristics of mature DC: MHC class II expression was markedly upregulated, mixed leukocyte reaction stimulatory activity was increased, and phagocytic function was decreased. (silverchair.com)
  • Additionally, there will be a lack of granulocytes, a type of white blood cell. (medicalnewstoday.com)
  • which represents the final cell type. (lu.se)
  • Until recently, and due to technical limitations, for a given cell type such experiments had to be done on an ensemble of many cells and, hence, gene expression quantifi- cations represented the average over a given population. (lu.se)
  • Type 3 is caused by mutations in the FERMT3 gene (11q13.1), which encodes kindlin-3 in hematopoietic cells. (msdmanuals.com)
Inflammation2
Counts3
  • The immature granulocyte counts ranged between 0 - 3.7 G/L by XN analyzer whereas 0 - 2.41 G/L by YH2500. (horiba.com)
  • The immature granulocyte counts per 100 WBC ranged between 0.1 - 11.2% by XN analyzer whereas 0.2 - 12% by YH2500. (horiba.com)
  • Granulocyte donors are almost always given medication regimens to increase the circulating granulocyte counts to improve collection. (medscape.com)
Plasma2
  • The blood of fish consists of plasma and the various cells that move around the body in it: erythrocytes and leukocytes. (earthlife.net)
  • Erythrocytes are by far the most common cells found in the blood plasma and their job is to move gases around the body. (earthlife.net)
Infection3
  • The number of granulocytes in the body usually increases when there is a serious infection. (medlineplus.gov)
  • Van Der GPJ, Mohseni M, Brouwer R, Van Der HB, Steyerberg EW, Groeneveld A. Immature granulocytes predict microbial infection and its adverse sequelae in the intensive care unit. (jsurgmed.com)
  • Vaccination resulted in robust cell-mediated and humoral responses and a significant reduction in lung lesions following SARS-CoV-2 infection. (bvsalud.org)
Receptor2
  • Chemokine activities were evaluated by measurement of intracellular calcium increase and by chemotasis and binding assays, using CXC chemokine receptor (CXCR)-transfected cell lines. (tau.ac.il)
  • Conversely, at the other boundary, immune hypersensitivity with gain of immune function in MHC class II-associated sJIA-AOSD and with chimeric antigen receptor (CAR) T cell therapy also triggers MAS. (nature.com)
Transient3
  • Figure 1: Transient, exuberant CD8 T cell expansion followed by CD4 T cell reconstitution. (ebmt.org)
  • BUdR treatment produced transient moderate myelosuppression that probably resulted from BUdR incorporation into normal marrow cells. (tmu.edu.tw)
  • PAF caused a rapid transient depolarization of the granulocyte membranes in the presence of extracellular Na+. (cdc.gov)
Antibodies1
  • seven.3 Discrimination utilizing particular antibodies-To detect either human or murine granulocytes it really is handy to start out with a staining for CD45 to define white blood cells, accompanied by simultaneous staining for CD11b. (flapinhibitor.com)
Leukemia3
  • Early T-cell expansion post T-replete mismatched cord transplant with granulocytes is associated with induction of remission and sustenance in relapsed-refractory high risk acute myeloid leukemia. (ebmt.org)
  • Stem cell transplant can be used to treat conditions such as multiple myeloma, and other cancers such as certain kinds of lymphoma and leukemia. (differencebetween.net)
  • For example, the presence of anemia along with thrombocytopenia with a low or high white blood cell count may suggest bone marrow involvement by leukemia. (medscape.com)
Clusters2
  • Events are plotted in the LMNE matrix where X-axis is the volume and Y-axis is optical extinction, showing several clusters of cells. (horiba.com)
  • Mononuclear cell progeny released from these clusters at and beyond 4 d exhibited distinct dendritic morphology and were actively phagocytic. (silverchair.com)
Human4
Scatter1
  • The side scatter (SSC) is deemed to get an indicator for the inner construction of the cell (e.g. nuclear morphology) as well as the forward scatter (FSC) reflects cellular size. (flapinhibitor.com)
Phagocytic1
  • Ehrlichia inclusion bodies, such as morulae, are visible in the cytoplasm of infected mononuclear phagocytic cells after 5-7 days. (medscape.com)
Inflammatory3
  • On Day 7 of stem cell transplant, he developed persistent, prolonged, high-grade fevers, oxygen requirement necessitating intensive care support and raised inflammatory markers with a CRP of 430mg/L and Serum Procalcitonin of 19mcg/L. Routine blood cultures were negative. (ebmt.org)
  • These chronic inflammatory cells then organise spatially into the classic granulomatous response 1 - 5 . (ersjournals.com)
  • Myeloid-derived suppressor cells (MDSCs) have a strong immunosuppressive character that allows them to regulate immune responses and hinder overt inflammatory responses. (lu.se)
Myeloid cell1
  • 2021) Myeloid-derived suppressor cells in the era of increasing myeloid cell diversity. (beckman.com)
Promyelocytes1
  • During an automated WBC differential count, the metamyelocytes, myelocytes and promyelocytes are counted as immature granulocyte (IMG) for many years previously by different HORIBA hematology analyzers while Sysmex analyzers report them as IG. (horiba.com)
Blasts1
  • The differential leukocyte count forms one of the most important parameters in quantifying different leukocytes including both mature and immature forms, detecting various morphological abnormalities or presence of unexpected atypical cells or blasts. (horiba.com)
Cellular2
  • Changes in SSC and FSC can also represent other morphological functions of many cellular processes (e.g. phagocytosis, cell death). (flapinhibitor.com)
  • these glycoproteins facilitate cellular interactions, cell attachment to blood vessel walls, cell movement, and interaction with complement fragments. (msdmanuals.com)
CD161
Significantly2
  • 1) In a multi-centric retrospective analysis of children who received stem cell transplant for high-risk AML, TRCB cohort had significantly improved event free survival and time to relapse and overall survival compared to the children who received stem cell transplant from other sources. (ebmt.org)
  • Treatment with GM-CSF significantly increased cell viability in a cell culture model of glutamate-induced neuronal injury. (elsevierpure.com)
Bone Marrow Tra4