• Small differences in binding kinetics between TCR ligands lead to marked differences in T cell effector function and differentiation. (nature.com)
  • Activation of mTOR-dependent pathways regulates the specification and differentiation of CD4 + T effector cell subsets. (jci.org)
  • 2016). Natural killer cell-based adoptive immunotherapy eradicates and drives differentiation of chemoresistant bladder cancer stem-like cells. (springer.com)
  • Transforming growth factor-β (TGF-β) signaling pathway is a multifunctional cytokine and plays important roles in regulating cell proliferation, cell cycle, differentiation, migration, and apoptosis in a broad spectrum of tissues [ 12 ]. (degruyter.com)
  • A research team from the University Hospitals of Cleveland Medical Center in western Pennsylvania has discovered the existence of myeloid neoplasms (MNs) that can undergo plasmacytoid dendritic cell (PDC) differentiation. (news-medical.net)
  • Both categories of cells resulting from atypical PDC differentiation are thought to represent two ends of a spectrum of maturation. (news-medical.net)
  • Leukemic myoblast differentiation into PDCs occurs as a result of sharing a common hematopoietic precursor to dendritic cells, monocytes, and myeloid cells. (news-medical.net)
  • By choosing appropriate antibodies, the differentiation of leukemic cells can be accurately determined. (wikipedia.org)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • The main advantage of these protocols is that they facilitate the isolation of human monocytes and their differentiation into viable and functional monocyte derived cells. (jove.com)
  • Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
  • In this study, highly purified, flow-cytometry sorted, classified in relation to normal B-cell differentiation [1]. (lu.se)
  • therefore, definitive differentiation of Hürthle cell carcinoma from Hürthle-cell adenoma is based on vascular invasion and/or capsular invasion, as well as on permanent histologic sections or extrathyroidal tumor spread and lymph node and systemic metastases. (medscape.com)
  • Early stages of B-cell differentiation can be identified by the status of the immunoglobulin genes and by the cell surface markers CD34, CD19, and surface immunoglobulin (sIg). (medscape.com)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Explore our flow cytometry guide to uncover flow cytometry basics, traditional flow cytometer components, key flow cytometry protocol steps, and proper controls. (sigmaaldrich.com)
  • Numerous types of labels can be used to visualize the antibodies for detection in the flow cytometer depending on the experimental design including nucleic acid dyes, cell viability dyes, polymer dyes, quantum dots, small organic molecules, and fluorescent proteins. (sigmaaldrich.com)
  • A flow cytometer is a device that tests cells by arranging them in a row and using a laser beam to measure the scattered light and fluorescence. (agc.com)
  • Products for use with flow cytometer instruments and flow cytometry procedures. (fishersci.com)
  • BD Accuri C6 Plus Flow Cytometer, with its compact footprint, light weight, and operational simplicity, supports a wide variety of applications including immunology, cell biology, plant and microbiology, and industrial applications. (fishersci.com)
  • Analyze with a flow cytometer. (immunochemistry.com)
  • The labelled cells are processed in a flow cytometer, a laser-based instrument capable of analyzing thousands of cells per second. (wikipedia.org)
  • Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. (immunochemistry.com)
  • that describe dual-platform technology, a method in which absolute counts are derived from measurements obtained from two instruments--a flow cytometer and hematology analyzer. (cdc.gov)
  • Uncover tips for multicolor flow cytometry experiments, including the challenges in multicolor flow cytometry, steps for how to build a multicolor flow cytometry panel, tandem dyes, and tools for multicolor assay design. (sigmaaldrich.com)
  • From flow cytometers and sorters for simple to complex research applications to an extensive selection of reagents, tools, educational resources and protocols, we support you in navigating your multicolor flow cytometry workflow journey. (bdbiosciences.com)
  • Numerous fields of study use flow cytometry and often analyze multiple parameters within diverse populations of cells. (sigmaaldrich.com)
  • Herein, we show that mTOR complex 1 (mTORC1) and mTORC2 have distinct roles in the generation of CD8 + T cell effector and memory populations. (jci.org)
  • Normal maturing PDC populations we found to make up less than 0.5% of adult bone marrow and are therefore difficult to detect by flow cytometry. (news-medical.net)
  • The overall goal of this study is to compare two different methods of human monocyte isolation for dendritic cell generation in vitro and to characterize the CT11c, CT14 cell surface expression of the resulting monocyte derived populations by imaging flow cytometry. (jove.com)
  • Flow cytometry offers multiparameter analysis of bacteria on a cell-by-cell basis to characterize populations. (purdue.edu)
  • It is particularly well-suited to natural populations of aquatic bacteria which are difficult to analyze by more traditional methods because of their small size (about 0.1µ m 3 ) and resistance to cultivation. (purdue.edu)
  • To determine if the immune cells that enter luteal tissue during structural regression are functionally different from those that are present during peak function, CD11b + or CD16 + populations were enriched from mid-late stage (functional) and regressing (days 1.8 ± 0.3 postmenses) CL using antibody-conjugated magnetic microbeads. (bioone.org)
  • Bone marrow osteoclast precursor cell populations were analyzed by flow cytometry from exposed animals. (cdc.gov)
  • In WT animals, exposure to inhalant ODE increased osteoclast precursor cell populations as compared to saline, an effect that was reduced in TLR4 but not TLR2 KO mice. (cdc.gov)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
  • Flow cytometry is used for single-cell analysis of cell populations and monitoring of population phenotypic heterogeneity as well as for preparative sorting of individual cells and subpopulations. (lu.se)
  • Little is known about the maternal immune cells at this site, which are in direct contact with fetal tissues. (frontiersin.org)
  • Together, these findings indicate that term placental tissues attract MAIT cells, and that this effect is at least partly mediated by MIF. (frontiersin.org)
  • However, the engineering of more complex tissues consisting of large 3D structures remains a critical challenge because the penetration of oxygen, which is required for cell survival, is limited by diffusion to a distance of approximately 150 to 200 mm from the nearest blood vessel. (hindawi.com)
  • Thus, the long-term survival and function of 3D tissues depend on the rapid development of new blood vessels to provide nutrients and oxygen to cells in the center of the tissue grafts. (hindawi.com)
  • To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor cell markers (CD44v6, CD24, and ALDH1A1) in NPC tissues and NPC cell lines. (hindawi.com)
  • The staining intensity of 8-nitroguanine was significantly higher in cancer cells and inflammatory cells in the stroma of NPC tissues than in chronic nasopharyngitis tissues. (hindawi.com)
  • Expression levels of CD44v6 and ALDH1A1 were significantly increased in cancer cells of primary NPC specimens in comparison to chronic nasopharyngitis tissues. (hindawi.com)
  • 8-Nitroguanine was detected in both CD44v6- and ALDH1A1-positive stem cells in NPC tissues. (hindawi.com)
  • ICT's FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. (immunochemistry.com)
  • In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
  • To prepare tissues for flow cytometry, mechanical and/or enzymatic disaggregation is required. (novusbio.com)
  • lamina propria and mesenteric lymph node tissues were analyzed by RNA sequencing and flow cytometry. (nih.gov)
  • Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
  • NK cell proliferation, phenotypic expression and cytotoxic activity were evaluated. (springer.com)
  • In its role as a cell signaling agent, GSH is involved in DNA synthesis and cell proliferation regulation. (immunochemistry.com)
  • Cell cycle analysis showed that visfatin increased S-stage percentage and proliferation index in a dose- and time-dependent manner. (thieme-connect.com)
  • In addition to providing biomechanical support to the islets, ECM components are required for β-cell development, proliferation, survival, and proper insulin secretion. (nature.com)
  • Further, the results of miR-4755-5p mimic transfection confirmed that under the action of NaF, miR-4755-5p overexpression reduced Cyclin D1 protein expression within osteoblasts and further inhibited cell proliferation and activation. (fluoridealert.org)
  • Transcriptome and chromatin immunoprecipitation analysis revealed c-Myc as a new and direct downstream target of XBP1 for regulation of NK cell proliferation. (cancerindex.org)
  • Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
  • In the present study, we assessed the possible effects of frankincense, pine needle and geranium essential oils on cell viability, proliferation, migration and invasion as well as the possible mechanisms. (spandidos-publications.com)
  • In the present study, we clearly revealed that frankincense, pine needle and geranium essential oils suppressed cell viability, proliferation, migration and invasion in human BC MCF-7 cells. (spandidos-publications.com)
  • Moreover, we elucidated the regulatory AMPK/mTOR pathway involving essential oils in BC cell proliferation, invasion and apoptosis development. (spandidos-publications.com)
  • The RAC serine/threonine-protein kinase (AKT) family of serine/threonine protein kinases, particularly the AKT1 isoform, has been identified abnormally expressed in hepatocellular carcinoma (HCC) cells, and is highly associated with cell behavior, including proliferation, survival, metabolism, and tumorigenesis. (spandidos-publications.com)
  • The purpose of the present study was to reveal the effects of AKT1 on the survival and proliferation of HCC cells, and to investigate the mechanisms involved. (spandidos-publications.com)
  • Molecular mechanisms and the influences of different regulation the expression of AKT1 on HCC cell growth, proliferation were determined by western blotting, MTT and colony formation assays, cell cycle and apoptosis were investigated by flow cytometry. (spandidos-publications.com)
  • The phosphoinositide 3-kinase (PI3K)/RAC seine/threonine-protein kinase (AKT)/mechanistic target of rapamycin (mTOR) signaling pathway is a promising therapeutic target owing to its frequent dysregulation in HCC and the critical functions it has in regulating cell survival, proliferation, apoptosis, migration and angiogenesis through phosphorylation of distinct protein substrates ( 6 , 7 ). (spandidos-publications.com)
  • AKT is a key molecule in the PI3K/AKT/mTOR signaling pathway, which has been shown to serve notable functions in the regulation of cell viability and to be closely associated with a variety of disorders caused by dysfunctional cellular proliferation ( 8 , 9 ). (spandidos-publications.com)
  • However, the precise molecular mechanisms by which AKT1 promotes cell proliferation and regulates apoptosis ( 18 , 19 ) remain largely unclear. (spandidos-publications.com)
  • High expression of activated AKT can be detected in HCC, and AKT may promote cell proliferation and regulation of cells apoptosis in HCC ( 20 , 21 ). (spandidos-publications.com)
  • Subsequent mechanism investigations revealed that AKT1 served a notable function in cell proliferation and anti-apoptosis by directly regulating the expression of phosphatase and tensin homolog (PTEN) and Notch1. (spandidos-publications.com)
  • T cells stimulate B cells to undergo further proliferation and Ig class switching, leading to the expression of the various isotypes IgG, IgA, or immunoglobulin E (IgE). (medscape.com)
  • Migration assays showed that MAIT cells and EM T cells migrated toward conditioned medium from placental explants. (frontiersin.org)
  • Blocking of MIF or a combination of MIF, CCL25, and CCL20 in migration assays inhibited MAIT cell migration toward placenta conditioned medium. (frontiersin.org)
  • Scientific Methods: In vitro growth inhibition assays were completed with AHCC alone and in combination with PLD in panel of human cancer cell lines and findings confirmed in vivo in an ovarian cancer xenograft mouse model. (researchgate.net)
  • View ColorWheel® protocol steps for flow cytometry analysis when using ColorWheel® antibodies with ColorWheel® dyes including antibody preparation, PBMC sample preparation, cell surface staining, and intracellular (cytoplasmic) staining. (sigmaaldrich.com)
  • Monocytes are the predominant innate immune cells at the early stage of Mycobacterium tuberculosis ( M. tb ) infection as the host defense against intracellular pathogens. (biomedcentral.com)
  • Due to the genetically conserved molecular processes by which cells die, intracellular levels of GSH can favor a cell death pathway via apoptosis (adequate intracellular GSH stores) or via necrosis or autophagy (depleted intracellular GSH stores). (immunochemistry.com)
  • Detection of a drop in intracellular GSH concentration in an experimental cell population relative to a negative (healthy cell) control is often indicative of an apoptosis induction event. (immunochemistry.com)
  • The kit provides all the essential reagents and an easy to follow protocol to assess changes in intracellular GSH levels using a flow cytometry analysis method. (immunochemistry.com)
  • The reduction in intracellular GSH concentration directly translates into an easily monitored reduction in the green fluorescence output within the dying or oxidatively stressed cell population. (immunochemistry.com)
  • The following flow cytometry staining protocol for intracellular molecules using detergents to permeabilize cell membranes has been developed and optimized by Bio-Techne. (novusbio.com)
  • For low cell density or poorly expressed intracellular targets, techniques like Single-Cell Westerns may be advantageous. (novusbio.com)
  • It is important to keep the cells in the presence of Permeabilization/Wash Buffer I during intracellular staining. (novusbio.com)
  • Herein, the role of gal-3 in regulating S. aureus phagocytosis and S. aureus -induced intracellular ROS was analyzed by imaging flow cytometry and luminol-based chemiluminescence, respectively. (lu.se)
  • induced intracellular ROS was analyzed by imaging flow cytometry and luminol-based chemiluminescence, respectively. (lu.se)
  • Since 1994, ImmunoChemistry Technologies has been producing top quality reagents, kits, and services for cell viability and ELISA applications in a broad range of research areas. (immunochemistry.com)
  • Cell viability was detected by CCK-8 method. (fluoridealert.org)
  • Cell viability dyes must be introduced before fixation and should be done at this point. (novusbio.com)
  • Cell count kit-8 (CCK-8) was used to observe cell viability. (bvsalud.org)
  • In vivo antibody depletion revealed a CD8 T-cell-dependent, and not NK cell-dependent mechanism of tumor growth inhibition after treatment with rapamycin and PD-L1 mAb, ruling out significant effects from NK cell-mediated antibody-dependent cellular cytotoxicity. (aacrjournals.org)
  • Individual experimental designs for flow cytometry must be optimized, including antibody dilution and incubation time. (novusbio.com)
  • Add 5-10 µL of conjugated antibody (or a previously titrated amount) per 1 x 10 6 cells and vortex. (novusbio.com)
  • 1 µL of primary antibody per 1 x 10 6 cells is a good starting point. (novusbio.com)
  • The antibody-enriched and depleted fractions were cultured for 24 h, and the media then analyzed for the production of 29 cytokines/chemokines. (bioone.org)
  • Defective antibody production and low circulating numbers of B cells were described in some female infants and in males in whom no Btk abnormalities were detected. (medscape.com)
  • First, we cocultured dermal fibroblasts with dermal microvascular endothelial cells at a ratio of 2 : 1. (hindawi.com)
  • A fibrous sheet was formed by the interactions between the fibroblasts and the endothelial cells, and capillary-like structures were observed after 20 days of coculture. (hindawi.com)
  • ELISA demonstrated that vascularization was promoted by significant upregulation of vascularization associated growth factors due to interactions among the 3 types of cells in the bilayer, as compared to cocultures of fibroblast and endothelial cells and monocultures. (hindawi.com)
  • Most of these methods involve culturing endothelial cells on or within ECM substrates (e.g., collagen, fibrin, fibronectin, laminin, etc.) or other types of biomaterials to form capillary-like structures [ 15 ]. (hindawi.com)
  • Visfatin Stimulates Production of Monocyte Chemotactic Protein-1 and Interleukin-6 in Human Vein Umbilical Endothelial Cells. (thieme-connect.com)
  • Here we examined whether ginkgolide B activated PXR and attenuated xenobiotic-induced injuries in endothelial cells. (greenmedinfo.com)
  • Human umbilical vein endothelial cells (HUVECs) were treated with ginkgolide B. The expression of PXR, CYP3A4, MDR1, VCAM-1, E-selectin and caspase-3 were quantified with qRT-PCR and Western blot analysis. (greenmedinfo.com)
  • Ginkgolide B exerts anti-apoptotic and anti-inflammatory effects on endothelial cells via PXR activation, suggesting that a PXR-mediated endothelial detoxification program may be important for protecting endothelial cells from xeno- and endobiotic-induced injuries. (greenmedinfo.com)
  • While some islet BM components are produced by endothelial cells (ECs), the source of others remains unknown. (nature.com)
  • MATERIALS AND METHODS: Male type 2 diabetic Sprague Dawley rats, Male D2.BKS(D)-Leprdb/J (db/db) mice, and mouse glomerular endothelial cells were utilized to evaluate the effect of icariin. (bvsalud.org)
  • Common molecular biology applications include analysis of recombinantly expressed fluorescent proteins that have induced expression in various cell types to elucidate gene function or for in vivo cell tracking. (sigmaaldrich.com)
  • Analyze transcripts and proteins simultaneously across thousands of cells at the single-cell level in the same workflow. (bdbiosciences.com)
  • It involves the labelling of white blood cells with antibodies directed against surface proteins on their membrane. (wikipedia.org)
  • In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
  • Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
  • However, cDC1 can be compromised in cancer patients and some cancer cells lack these antigen-presenting proteins on their surface, impacting immunotherapy response. (lu.se)
  • Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
  • Flow cytometry is a powerful technology that is commonly used in molecular and cellular biology research applications, including immunology, cancer biology, and disease research. (sigmaaldrich.com)
  • International Journal of Biochemistry & Cell Biology, 40 , 2335-2340. (springer.com)
  • Natural killer cell biology: An update and future directions. (springer.com)
  • University of Oklahoma researcher publishes a study identifying 135 previously unknown pigmentation genes with broad implications for human diversity, cell biology and medicine. (ou.edu)
  • Flow cytometry have important applications in many fields including fundamental and applied microbiology, bioprocess monitoring, protein engineering, plant biology and marine biology. (lu.se)
  • Learn key steps in flow cytometry protocols to make your next flow cytometry experiment run with ease. (sigmaaldrich.com)
  • If working with adherent cells, please see the manual for additional protocols. (immunochemistry.com)
  • These monocytes isolation protocols can contribute to the development of reliable methods for the purification of human dendritic cells for research and clinical applications. (jove.com)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
  • Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)
  • The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expansion of NK cells. (springer.com)
  • In vitro CD4+ (E) and CD8+ (F) T cells production of TNF, IL-2, IFN, Granzyme B (GZB) and IL-17, after unspecific stimulation with PMA/ionomycin, before and after nivolumab injection in patient 3. (cdc.gov)
  • This in vitro assay employs the fluorescent inhibitor probe FAM-WEHD-FMK to label active caspase-1 enzyme in living cells. (immunochemistry.com)
  • This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). (jove.com)
  • Consistent with the in vitro activities, frankincense essential oil was effective in inhibiting tumor growth and inducing tumor cell apoptosis in a human BC mouse model. (spandidos-publications.com)
  • Using in vitro cell cultures, Bajpai developed a novel method to achieve this goal that detects and quantifies the melanin-producing activity of melanocytes. (ou.edu)
  • In vitro, we utilized high glucose stress to induce impairment in glomerular mesangial cells (GMCs) as diabetic nephropathy cell model. (bvsalud.org)
  • qPCR, Western blot, immunofluorescence staining and flow cytometry were used to evaluate the effect of PUR on the generation of extracellular matrix (ECM), ferroptosis and iron homeostasis in vitro and in vivo. (bvsalud.org)
  • Described here are some of the general requirements of in vitro test methods for skin sensitization, and progress that has been made in developing suitable approaches with particular emphasis on the utility of dendritic cell culture systems. (cdc.gov)
  • However, our recent findings showed that mucosal-associated invariant T (MAIT) cells and effector memory (EM) T cells are enriched in the IVB of term placentas ( 4 ), indicating that certain immune cell subsets are recruited to or retained in the intervillous space. (frontiersin.org)
  • The potential factors involved in the migration of maternal immune cells to the placenta is still unexplored. (frontiersin.org)
  • We aimed for addressing the role of FasL expression in human intervertebral disc degeneration (IDD) and immune privilege in terms of the interaction between NP cells and immunocytes via the FasL-Fas machinery. (medsci.org)
  • The peri-islet membrane, which encapsulates the islets, separates the endocrine cells from the exocrine pancreas and serves as a barrier from immune cell infiltration to the islets. (nature.com)
  • Among the responders to microbial invasion, neutrophils represent the earliest and perhaps the most important immune cells that contribute to host defense with the primary role to kill invading microbes using a plethora of stored anti-microbial molecules. (lu.se)
  • One soluble factor modulating the interplay between immune cells and microbes is galectin-3 (gal-3), a carbohydrate-binding protein that regulates a wide variety of neutrophil functions. (lu.se)
  • Her research involves direct reprogramming approaches, which function like a Trojan horse, enabling cancer cells to be targeted by the immune system. (lu.se)
  • Immunotherapy can fail in patients with cancer when certain cancer cells can hide from the immune system. (lu.se)
  • By using direct reprogramming strategies, I could induce the expression of tumor antigens on the surface of cancer cells, making them visible to the immune system again. (lu.se)
  • when it comes to cancer they are critical as they present tumor antigens to the killer cells of the immune system and orchestrate the anti-tumor immune response. (lu.se)
  • Direct reprogramming strategies with cDC1-specific factors can induce antigen-presentation in cancer cells, making cancer cells visible to the immune system and easier to fight. (lu.se)
  • The course covers the structure and function of the immune system, as well as immunological processes at different levels (organism, organ, cell and molecular levels). (lu.se)
  • Unfixed samples may also be analyzed with Propidium Iodide or Hoechst 33342 to detect necrosis or changes in nuclear morphology, respectively. (immunochemistry.com)
  • Isolation, expansion, and characterization of natural killer cells and their precursors as a tool to study cancer immunosurveillance. (springer.com)
  • Loss of the ECM during the islet isolation process is considered to be a significant factor in the poor engraftment rate of transplanted β-cells, thus limiting cell replacement therapy to diabetes 6 . (nature.com)
  • This method can also be applied as an alternative for the isolation of other rare cells at an adequate yield for downstream research applications. (jove.com)
  • T cells have the ability to sense and discriminate a wide range of antigenic peptides bound to major histocompatibility complex (pMHC) molecules according to their affinity for the T cell receptor (TCR). (nature.com)
  • Expression of surface T cells inhibitory molecules in Patient 2 and 3 at diagnosis and after nivolumab injection. (cdc.gov)
  • Phenotypic analysis of T-cells inhibitory molecules (PD-1, 2B4, CD160) expression in CD4+ (A) and CD8+ (B) cel s before and after nivolumab injection in patient 2. (cdc.gov)
  • Our facilities provide the opportunity to study molecules, cells, organs and entire organisms. (lu.se)
  • We used RNA sequencing coupled with chromatin immunoprecipitation sequencing to analyze primed microglial inflammatory gene expression and modifications to histone H3 lysine 4 trimethylation (H3K4me3) at the promoters of primed genes. (frontiersin.org)
  • In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
  • Explore comprehensive single-cell workflow solutions to increase your experimental power. (bdbiosciences.com)
  • Experimental validation suggested that PUR treatment could inhibit the generation of ECM to alleviate high-glucose-induced cell impairments, suppressing ROS production and excessive collagen fiber accumulation in GMSs, and reduce mesangial matrix expansion and renal fibrosis in type 2 DN rats. (bvsalud.org)
  • Immunophenotyping is a technique used to study the protein expressed by cells. (wikipedia.org)
  • The process of measuring the percentage of CD4+ T-lymphocytes in the whole blood sample is referred to as ``immunophenotyping by flow cytometry'' (9-14). (cdc.gov)
  • Flow cytometric immunophenotyping is a highly complex and relatively new technology. (cdc.gov)
  • This document provides CDC recommendations for immunophenotyping by flow cytometry. (cdc.gov)
  • Using immunophenotyping, HIV-positive blood samples and age-matched controls were tested for the proportion of lymphocytes that are T cells, B cells, natural killer (NK) cells, CD4+ T cells (helper T cells), and CD8+ T cells (suppressor/inducer T cells). (cdc.gov)
  • A multivariate factor analysis indicated that high levels of MIF and CCL25 were associated with high proportions of MAIT cells in intervillous blood. (frontiersin.org)
  • Flow cytometry is a technology that uses single or multiple lasers to provide a multi-parametric analysis of single cells. (sigmaaldrich.com)
  • Need tips on how to improve your flow cytometry analysis? (sigmaaldrich.com)
  • And macrophages and CD8 + T cells were harvested for apoptosis analysis by FCM after 2 days of co-cultures. (medsci.org)
  • FCM analysis found that human NP cells with increased FasL expression resulted in significantly increased apoptosis ratio of macrophages and CD8 + T cells. (medsci.org)
  • Microfluidic devices are also sometimes referred to as μTAS (micro total analysis systems), lab-on-a-chip, or flow cells, but are generally referred to as microfluidic devices. (agc.com)
  • In both analysis types, all PDCs showed partial to moderate expression of the cell marker CD34 indicating premature PDCs. (news-medical.net)
  • From specimen collection to sample preparation to cell sorting and analysis, BD Biosciences offers a multitude of tools for immuno-oncology research. (bdbiosciences.com)
  • Distributions of marine isolate Cycloclasticus oligotrophus grown at two different rates (µ) under acetate-limiting conditions for cell cycle analysis. (purdue.edu)
  • However, most GEP studies have typically been performed on whole tissue samples, containing varying degrees of tumor cell content, which results in uncertainties in data analysis. (lu.se)
  • Flow cytometry analysis of forebrain-derived and developing CNS in rodents (Rietze et al. (lu.se)
  • Focus of the course will be on getting practical experience of flow cytometry for single-cell analysis and cell sorting of microorganisms. (lu.se)
  • SPT incorporates internal calibrator beads of known quantity in the analysis of specimens by three- or four-color flow cytometry. (cdc.gov)
  • Here, we used time-resolved high-throughput proteomic analyses to identify and quantify the phosphorylation events and protein-protein interactions encoding T cell ligand discrimination in antigen-experienced T cells. (nature.com)
  • Flow cytometry analyses revealed the majority of CD11b + cells expressed CD14, a protein mainly produced by macrophages/monocytes. (bioone.org)
  • GEP analyses were performed on highly purified, flow-cytometry sorted tumor-cells from eight subgroups of BCLs. (lu.se)
  • Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses using three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions and identify 13 IAV-modulated kinases. (cdc.gov)
  • The presence of cancer of the lymphatic system is often determined by analyzing samples from the blood or bone marrow. (eurekalert.org)
  • MSCs were obtained from the bone marrow (BM) cells remaining in the bag and filter used in the transplantation of hematopoietic stem cells. (springer.com)
  • The group analyzed seven MN patients who presented with PDC proliferations comprising 5-26% of that blood cell or bone marrow. (news-medical.net)
  • The whole procedure can be performed on cells from the blood, bone marrow or spinal fluid in a matter of a few hours. (wikipedia.org)
  • Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
  • First, swine facility ODE stimulated wild-type (WT) bone marrow macrophages to form osteoclasts, and this finding was inhibited in TLR4 knock-out (KO), but not TLR2 KO cells. (cdc.gov)
  • In the fetal bone marrow, the first committed cell in B-cell development is the early pro-B cell, identified by its ability to proliferate in the presence of interleukin-7 (IL-7). (medscape.com)
  • The completion of rearrangement of the light and heavy chains and the presence of surface IgM results in the immature B cell, which then leaves the bone marrow. (medscape.com)
  • This whole cell glutathione assay employs a proprietary thiol-sensitive dye, ThioBright™ Green, to monitor relative changes in the concentration of the free form of glutathione, aka GSH. (immunochemistry.com)
  • The key assay reagent is a proprietary thiol-sensitive dye, ThioBright™ Green, which quickly penetrates cell membrane structures and accumulates primarily within the cytosol of living cells. (immunochemistry.com)
  • Optional: Spin and pellet cells, remove supernatants, and gently resuspend in Assay Buffer or other isotonic buffer. (immunochemistry.com)
  • Furthermore, cancer cells undergoing EMT phenotypic changes also acquire drug resistance [ 7 ]. (techscience.com)
  • Furthermore, A. fumigatus particles exhibited greater persistence in the lungs of recipient mice when compared to non-viable A. fumigatus or A. versicolor , and this correlated with increased maintenance of airway memory-phenotype CD8 + T cells. (cdc.gov)
  • Flow cytometry is a high-throughput technique for counting and studying microscopic particles in suspension by passing them through a laser and an electronic detection apparatus. (lu.se)
  • Check out this flow cytometry troubleshooting guide with possible causes and solutions for most common problems encountered during flow cytometry experiments, including a loss or lack of signal, high background, decreasing acquisition rates, and more. (sigmaaldrich.com)
  • BD ® Research Cloud is a unique ecosystem for flow cytometry experiments to drive visibility, teamwork and efficiency for your lab. (bdbiosciences.com)
  • These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)
  • This work builds on existing knowledge that PDCs can be divided into two categories: mature PDC proliferations which result in myeloid neoplasms (MPDMN) and blastic plasmacytoid dendritic cell neoplasm (BPDCN). (news-medical.net)
  • Alexandra Gabriela Barros Ferreira defends her Ph.D. thesis "Restoring antitumor immunity with dendritic cell reprogramming strategies" on Friday, 17 November, 2023. (lu.se)
  • My Ph.D. studies have focused on restoring anti-tumor immunity with dendritic cell reprogramming strategies. (lu.se)
  • Such markers can also be used to detect small differences between cancer cells and healthy blood cells. (eurekalert.org)
  • These data are then typically analyzed on screen by plotting the expression of the markers used against each other. (eurekalert.org)
  • In conclusion, CD44v6 and ALDH1A1 are candidate stem cell markers for NPC, and the increased formation of DNA lesions by inflammation may result in the mutation of stem cells, leading to tumor development in NPC. (hindawi.com)
  • They examined multiple markers of cell identity (CD22, CD123, CD303, and CD304) to determine the subgroups of PDCs. (news-medical.net)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • 2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)
  • Detection and accurate characterization of pathological cells is important when making a diagnosis. (eurekalert.org)
  • Cell sorting technology is also used in the detection of rare cells. (agc.com)
  • PDC progenitors are in common with monocytic and myeloid cell progenitors. (news-medical.net)
  • Controversy exists about the origin of Hürthle cells, which generally are thought to derive from the follicular epithelium. (medscape.com)
  • In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
  • To identify which genes influence melanin production, cells that lost melanin during the gene removal process needed to be separated from millions of other cells that did not. (ou.edu)
  • These separated cells were then analyzed to determine the identity of melanin-modifying genes. (ou.edu)
  • Genes required for B cell development. (medscape.com)
  • These cells develop into late pro-B cells in which rearrangement of the heavy chain genes occurs. (medscape.com)
  • Progression from this late pro-B-cell to the pre-B-cell stage involves the rearrangement and joining of the various segments of the heavy chain genes. (medscape.com)
  • Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)
  • In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)
  • T-cell numbers were derived from three measurements deter- attributable to the decrease in the number of T cells that bear mined with two different instruments, a hematology analyzer the CD4 receptor ( 5-9 ). (cdc.gov)
  • FasL expression was detected by qRT-PCR, western blotting and flow cytometry (FCM). (medsci.org)
  • Surface expression of CD14, CD16, and CD163 on monocytes was detected using flow cytometry. (biomedcentral.com)
  • The level of miR-128-3p was decreased, and TGFBR2 expression was increased in serum samples of sepsis patients and LPS-induced HK2 cells. (degruyter.com)
  • Ginkgolide B increased the expression of CYP3A4 and MDR1 in the cells, which was partially reversed by pretreatment with the selective PXR signaling antagonist sulforaphane, or transfection with PXR siRNA. (greenmedinfo.com)
  • Moreover, ginkgolide B suppressed TNF-α-induced THP-1 cell adhesion and TNF-α-induced expression of vascular adhesion molecule 1 (VCAM-1) and E-selectin in the cells, which was also reversed by transfection with PXR siRNA. (greenmedinfo.com)
  • Whereas α2 laminins promoted islet cell clustering, they did not affect gene expression. (nature.com)
  • Herein, we demonstrate apabetalone, the most clinical advanced BET inhibitor, downregulates expression of cell surface receptors involved in SARS-CoV-2 entry, including angiotensin-converting enzyme 2 (ACE2) and dipeptidyl-peptidase 4 (DPP4 or CD26) in SARS-CoV-2 permissive cells. (biorxiv.org)
  • Western blotting and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) to evaluate the expression levels of AKT1 in HCC SMMC‑7721 cell line. (spandidos-publications.com)
  • Furthermore, the data of the present study indicated that B‑cell lymphoma 2 and cyclin D1 is involved in the regulation of AKT1 expression. (spandidos-publications.com)
  • Increasing expression of IgD in the transitional cells finally results in the mature B cell with IgM and IgD both expressed on their cell surface. (medscape.com)
  • When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)
  • The FLICA reagent FAM-WEHD-FMK enters each cell and irreversibly binds to activated caspase-1. (immunochemistry.com)
  • Because the FAM-WEHD-FMK FLICA reagent becomes covalently coupled to the active enzyme, it is retained within the cell, while any unbound FAM-WEHD-FMK FLICA reagent diffuses out of the cell and is washed away. (immunochemistry.com)
  • The remaining green fluorescent signal is a direct measure of the active caspase-1 enzyme activity present in the cell at the time the reagent was added. (immunochemistry.com)
  • Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the kit. (immunochemistry.com)
  • Epithelial cells were then seeded on the fibrous sheet to assemble the bilayered tissue. (hindawi.com)
  • Similarly, more intense staining of CD44v6 and ALDH1A1 was detected in an NPC cell line than in an immortalized nasopharyngeal epithelial cell line. (hindawi.com)
  • Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
  • Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
  • MAIT cells are an invariant type of T cell that respond to microbial derived metabolites from riboflavin synthesis presented by the non-polymorphic MHC class I related molecule (MR1) ( 5 ). (frontiersin.org)
  • In the course we will cover the most important applications of microbial flow cytometry and provide a clear view of what kind of information can be obtained, as well as how to analyze and interpret the data. (lu.se)
  • Flow cytometry is a widely used method for analyzing and quantifying cells. (innov-research.com)
  • It is widely used in institutions that handle cells. (agc.com)
  • CD16 + cells (presumptive NK cells) were observed mainly near the vasculature in functional CL, until their numbers increased and they became widely distributed in regressing CL. (bioone.org)
  • Although defects may occur in many steps in B-cell development and maturation resulting in the lack of Ig production, the most common and well-described defect is the one at the stage of pro-B-cell to pre-B-cell maturation (see the image below). (medscape.com)
  • Type 3 is caused by mutations in the FERMT3 gene (11q13.1), which encodes kindlin-3 in hematopoietic cells. (msdmanuals.com)
  • Recently, evidence has accumulated showing that stem cells are involved in inflammation-related carcinogenesis. (hindawi.com)
  • rather, this property is limited to a subset of cells termed "cancer stem cells" [ 15 , 16 ]. (hindawi.com)
  • A subset of cancer cells undergoing EMT acquire characteristics of stem cells and are designated as cancer stem cells (CSCs) [ 8 ]. (techscience.com)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
  • This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. (cancerindex.org)
  • An estimated 90% of patients with early-onset agammaglobulinemia and absence of B cells have abnormalities in the Btk gene (ie, Bruton agammaglobulinemia or XLA). (medscape.com)
  • The defect of µ heavy-chain gene on chromosome 14 is the most frequent abnormality in patients with agammaglobulinemia and decreased B cells but no defect in Btk. (medscape.com)
  • Autosomal Recessive Genetic disorders determined by a single gene (Mendelian disorders) are easiest to analyze and the most well understood. (msdmanuals.com)
  • Many cancers re-emerge after treatment, despite the sensitivity of the bulk of tumor cells to treatments. (mdpi.com)
  • This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). (lu.se)
  • In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)
  • Lateral flow immunoassay (LFIA) is a membrane-based technique for detecting specific analytes in complex samples. (jacksonimmuno.com)
  • During erythropoiesis, the cells start to produce hemoglobin, lose nuclei and organelles, and induce plasma membrane remodeling. (elifesciences.org)
  • β-Cells depend on the islet basement membrane (BM). (nature.com)
  • Thus, human endocrine cells are not in direct contact with the vascular BM components but with the invaginated peri-islet membrane. (nature.com)
  • Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)
  • However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)
  • 2014). Human NK cells: From surface receptors to the therapy of leukemias and solid tumors. (springer.com)
  • NK expansion and activation were stimulated by culture with artificial antigen-presenting cells (aAPCs) and IL-2, in the presence or absence of BM-MSCs. (springer.com)
  • Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells. (immunochemistry.com)
  • Flow Cytometry Permeabilization/Wash Buffer I (R&D Systems, Cat No. FC-005) or an equivalent solution containing a permeabilization agent such as Saponin, Triton ® X-100 or Tween ® 20 (0.1-0.5% in your wash buffer). (novusbio.com)
  • Resuspend cell pellets in 150 μL of Flow Cytometry Permeabilization/Wash Buffer I. Add 1 μg blocking IgG per 1 x 10 6 cells and let stand for 15 minutes at room temperature. (novusbio.com)
  • Wash cells ONCE with 2 mL of Flow Cytometry Permeabilization/Wash Buffer I. Centrifuge suspension at 1,300 RPM (500 x g maximum) and 4°C for 5 minutes. (novusbio.com)
  • Analyze the fluorescent signal by flow cytometry. (immunochemistry.com)
  • During periods of oxidative stress or GSH depletion associated with cell death processes, cytosolic concentrations of the green fluorescent dye form become significantly diminished. (immunochemistry.com)
  • Multiline output is critical because it allows selection of the wavelength that offers the highest excitation cross section for the multiple fluorescent labels that are employed in cell imaging. (photonics.com)
  • Overall, this study provides a comprehensive map of molecular events associated with T cell ligand discrimination. (nature.com)
  • Understanding of molecular mechanisms in natural killer cell therapy. (springer.com)
  • Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-fluorouracil (5-FU). (techscience.com)
  • Flow cytometry generates large amounts of data. (eurekalert.org)
  • Gal-3 has been shown to potentiate neutrophil interaction with bacteria, including Staphylococcus aureus , and is also a potent activator of the neutrophil respiratory burst, inducing large amounts of granule-localized ROS in primed cells. (lu.se)
  • and is also a potent activator of the neutrophil respiratory burst, inducing large amounts of granule-localized ROS in primed cells. (lu.se)
  • We have characterized the T cell composition and chemokine profile in paired intervillous and peripheral blood samples from healthy mothers giving birth following term pregnancies. (frontiersin.org)
  • Mucosal-associated invariant T (MAIT) cells and effector memory (EM) T cells were enriched in the intervillous blood compared to peripheral blood, suggesting that MAIT cells and other EM T cells home to the placenta during pregnancy. (frontiersin.org)
  • Furthermore, pregnant women had lower proportions of peripheral blood MAIT cells compared to non-pregnant women. (frontiersin.org)
  • The intervillous blood (IVB) is exchanged 2-3 times per minute ( 1 ), suggesting that the cell composition in IVB may reflect that of peripheral blood (PB). (frontiersin.org)
  • The aim of our research was to determine how immunotherapy changes the proportion of lymphocyte subsets in dog peripheral blood and the levels of cytokines secreted by these cells during therapy. (mdpi.com)
  • We also collected macrophages and CD8 + T cells from the peripheral blood of patients with IDD for co-cultures with NP cells. (medsci.org)
  • NK cells were obtained from peripheral blood (PB) of healthy volunteers. (springer.com)
  • To achieve a proper separation of the peripheral blood mononuclear cells, load the blood onto the density gradient solution quickly, but carefully and without mixing the layers. (jove.com)