Puromycin is an antibiotic protein synthesis inhibitor which causes premature chain termination during translation. (wikipedia.org)
The antibiotic puromycin (Puro) is a protein synthesis inhibitor causing premature polypeptide chain termination during translation. (bvsalud.org)
Antibiotic1
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. (wikipedia.org)
Causes premature1
Deletion of AP4 causes premature senescence and defects in mitogen-induced proliferation in mouse embryonic fibroblasts (MEFs) [ 5 , 7 ]. (biomedcentral.com)
Plates containing puromycin are stable for 1 month when stored at 4 °C.[citation needed] Puromycin resistance in yeast can also be conferred through expression of the puromycin N-acetyl-transferase (pac) gene. (wikipedia.org)
Termination1
2016). Further, it is crucial to avoid shRNAs containing a poly(T) tract longer than four nucleotides, as this could lead to premature shRNA termination (TRC library shRNA are pre-screened to avoid this problem). (berterolab.com)
Cell3
Puromycin is used in cell biology as a selective agent in cell culture systems. (wikipedia.org)
Puromycin is stable for one year as solution when stored at -20 °C. The recommended dose as a selection agent in cell cultures is within a range of 1-10 μg/ml, although it can be toxic to eukaryotic cells at concentrations as low as 1 μg/ml. (wikipedia.org)
Lethal concentrations of puromycin are much higher for strains of Saccharomyces cerevisiae than mammalian cell lines. (wikipedia.org)
Loss2
As puromycin inhibits protein synthesis in eukaryotic cells, researchers were able to show that injections of this drug will result in both short-term as well as long-term memory loss in mice. (wikipedia.org)
We found that mutation of Tyr31/118 caused enhanced activation of RhoA and premature formation of stress fibers with substantial loss of efficient membrane spreading and ruffling in adhesion and migration of NMuMG cells. (silverchair.com)
Formation1
It enters the A site and transfers to the growing chain, causing the formation of a puromycylated nascent chain and premature chain release. (wikipedia.org)
Selection1
For hassle-free selection and optimum results the use of specially modified puromycin is possible. (wikipedia.org)
In this reaction, a puromycin molecule is chemically attached to the end of an mRNA template, which is then translated into protein. (wikipedia.org)
As puromycin inhibits protein synthesis in eukaryotic cells, researchers were able to show that injections of this drug will result in both short-term as well as long-term memory loss in mice. (wikipedia.org)
Puromycin is a protein synthesis inhibitor. (fermentek.com)
The mutation causes a premature stop codon in the mRNA sequence, which results in abnormal protein production and separation of skin layers. (helsinki.fi)
The puromycin can then form a covalent link to the growing peptide chain allowing the mRNA to be physically linked to its translational product. (wikipedia.org)
Concentrations3
Puromycin is stable for one year as solution when stored at -20 °C. The recommended dose as a selection agent in cell cultures is within a range of 1-10 μg/ml, although it can be toxic to eukaryotic cells at concentrations as low as 1 μg/ml. (wikipedia.org)
Lethal concentrations of puromycin are much higher for strains of Saccharomyces cerevisiae than mammalian cell lines. (wikipedia.org)
At low concentrations, puromycin enhanced flux into rapidly degraded polypeptide pools, with small polypeptides being markedly more labile then high molecular weight puromycin adducts. (nih.gov)
Cell biology1
Puromycin is used in cell biology as a selective agent in cell culture systems. (wikipedia.org)
Gene1
Deletion of the gene encoding the multidrug efflux pump Pdr5 sensitizes cells to puromycin. (wikipedia.org)
Cellular1
Additionally, cav-1-overexpressing murine embryonic fibroblasts (MEFs) display a large, flat morphology and express high levels of senescence-associated β-galactosidase activity, revealing premature cellular senescence [ 21 ]. (biomedcentral.com)
Prevents1
In the late phases, M2 prevents premature activation of newly synthesized HA [ 1 ] and -in concert with M1- contributes to virus budding and morphology. (biomedcentral.com)
Cells2
Puromycin acts quickly and can kill up to 99% of nonresistant cells within 2 days. (wikipedia.org)
Oxytocin receptor antagonists, such as barusiban or atosiban have the opposite effect of depressing oxytocin receptor activity and can be used to stop premature labor and uterine contractions. (mdwiki.org)
Solution1
Puromycin is soluble in water (50 mg/ml) as colorless solution at 10 mg/ml. (wikipedia.org)
Quality1
Pain and reduced mobility in OA patients bring much more than a drastic decline in quality of life, but also increased risk of premature death due to cardiovascular disease, diabetes mellitus, obesity, and cognitive disorders (2). (researchsquare.com)