• This naturally occurring bacterium, commonly found in insects, works its magic by rendering the Aedes aegypti mosquito sterile when present in their bodies. (expresshealthcaremgmt.com)
  • The researchers released adult Aedes aegypti mosquitoes infected with Wolbachia bacteria in three areas near to suitable mosquito habitat in Cairns, Queensland. (ist.ac.at)
  • 2017): Local introduction and heterogeneous spatial spread of dengue-suppressing Wolbachia through an urban population of Aedes aegypti. (ist.ac.at)
  • Researchers at the University of Wisconsin-Madison have confirmed that a benign bacterium called Wolbachia pipientis can completely block transmission of Zika virus in Aedes aegypti , the mosquito species responsible for passing the virus to humans. (scienceblog.com)
  • While not typically found in the Aedes aegypti mosquito - the species that also transmits dengue, chikungunya and yellow fever viruses - O'Neill discovered in the early 1990s that Wolbachia could be introduced to the mosquito in the lab and would prevent the mosquitoes from transmitting dengue virus. (scienceblog.com)
  • Zika virus belongs to the same family as dengue virus and Aliota and Osorio - with co-authors Stephen Penaido at SVM and Ivan Dario Velez, at the Universidad de Antioquia in Medellin, Colombia - asked whether Wolbachia -harboring Aedes aegypti may also be effective against Zika virus. (scienceblog.com)
  • We saw reduced vector competence in Aedes aegypti with Wolbachia ," says Osorio, defined as the intrinsic ability of an insect to support the development or replication of a pathogen like a virus and then transmit it. (scienceblog.com)
  • Researchers released batches of Aedes aegypti mosquitoes carrying the w AlbB strain of Wolbachia into the wild, in six different sites in greater Kuala Lumpur with high levels of dengue transmission. (gla.ac.uk)
  • The low-cost, 3D-printed portable toolbox identifies a pair of genes in a crushed-up mosquito that reveal whether the insect is Aedes aegypti and whether it has been exposed to the Wolbachia bacterium, which prevents the spread of diseases carried by the mosquito. (mongabay.com)
  • DIY Diagnostics , a team of researchers based at University of Texas at Austin, has been investigating how the Wolbachia bacterium can be used to suppress the load of dangerous viruses transmitted to humans by one of the most notorious mosquito species, Aedes aegypti . (mongabay.com)
  • The diagnostic tool uses a 3D-printed box, a smartphone camera and a simple chemical test to determine whether a mosquito is Aedes aegypti and whether it has been exposed to the Wolbachia bacterium. (mongabay.com)
  • The only change is the strain of Wolbachia being implanted currently occurs in another mosquito genus (Aedes). (hawaiifreepress.com)
  • It turns out that Wolbachia prevents these viruses from reproducing in Aedes aegypti mosquitoes that spread them. (phys.org)
  • When a wolbachia-infected male mates with a wild female Aedes aegypti, the resulting eggs do not hatch. (bigpinekey.com)
  • A promising candidate for arbovirus control and prevention relies on replacing arbovirus-susceptible Aedes aegypti populations with mosquitoes that have been colonized by the intracellular bacterium Wolbachia and thus have a reduced capacity to transmit arboviruses. (bvsalud.org)
  • Some strains of the inherited bacterium Wolbachia have been shown to be effective at reducing the transmission of dengue virus (DENV) and other RNA viruses by Aedes aegypti in both laboratory and field settings and are being deployed for DENV control. (bvsalud.org)
  • Symbiotic bacteria have played crucial roles in the evolution of sap-feeding insects and can strongly affect host function. (researchgate.net)
  • Its pathogenic cousins all host the symbiotic bacteria Wolbachia inside their cells, but Loa loa does not. (broadinstitute.org)
  • Bordenstein began studying the WO 15 years ago because he was curious about how such a virus survives and flourishes in a symbiotic bacteria like Wolbachiathat has a very small genome. (phys.org)
  • Many species of phloem feeding insects are host to symbiotic bacteria, which provide their hosts with nutrients lacking from phloem. (umd.edu)
  • Bacteria that cannot be grown in pure cultures make up a large part of the biomass and genetic diversity on earth and play an important role in many system, for example in the many symbiotic associations with eukaryotes. (uu.se)
  • My research focuses on symbiotic bacteria that cannot live outside of a eukaryotic host cell. (uu.se)
  • My main interests are in finding out how these tight associations affect genome evolution, determining genetic factors involved in host interactions and exploring the diversity of symbiotic bacteria in nature. (uu.se)
  • In order to do so, many insect species have developed very tight symbiotic relationships with microorganisms such as bacteria and fungi that provide them with the nutrients that are lacking in their diet. (uu.se)
  • Apart from these nutritional interactions, many insects carry additional symbiotic bacteria of which we currently know quite little, but that in some insects have been shown to provide adaptation for specific conditions. (uu.se)
  • WOLBACHIA, induces changes in sperm of the host, causing mitotic defects in the fertilized embryo only in crosses between symbiotic males and females harboring a different or no symbiont. (bvsalud.org)
  • Amid concerns about the initial rise in mosquito populations following the release of Wolbachia-infected larvae, the Ministry of Health reassures the community that this is simply a temporary phenomenon. (expresshealthcaremgmt.com)
  • Lateral phage transfer in obligate intracellular bacteria (wolbachia): verification from natural populations. (mbl.edu)
  • The researchers tracked the spread of Wolbachia through mosquito populations over two years by trapping and testing them for the bacteria. (ist.ac.at)
  • A bacterium named Wolbachiapipientis naturally infects many insect species and has the ability to interfere with its host's reproductive ability in such a way that entire populations become infected within just a few generations i . (real-agenda.com)
  • This research, together with previously reported infections in D. melanogaster and D. simulans populations, indicate that Wolbachia infects 3 out of 9 Drosophila species surveyed in Ukraine. (ub.edu)
  • Cooper said the World Health Organization recommends further developing Wolbachia biocontrol efforts like the World Mosquito Program , which aims to protect 500 million people from disease by 2030 by establishing pathogen-blocking Wolbachia in mosquito populations. (umt.edu)
  • We will unravel the genetic interactions between Wolbachia and their hosts," Cooper said, "as well as interactions with environmental factors like temperature, which influence Wolbachia prevalence in host populations. (umt.edu)
  • Here, we investigate whether horizontal transmission of Wolbachia can explain clonal genetic variation in natural populations of Leptopilina clavipes, a parasitoid wasp infected with a parthenogenesis-inducing Wolbachia. (datadryad.org)
  • Therefore, to understand the influence of Wolbachia on within-host ZIKV evolution, we characterized the genetic diversity of molecularly barcoded ZIKV virus populations replicating in Wolbachia-infected mosquitoes and found that within-host ZIKV evolution was subject to weak purifying selection and, unexpectedly, loose anatomical bottlenecks in the presence and absence of Wolbachia. (bvsalud.org)
  • Suppression efforts have mainly focused on mosquito control, but an international, non-profit research collaboration, the Eliminate Dengue Program, is trialing a new approach: harnessing bacteria that infect mosquitoes and so reduce their capacity to transmit viruses. (ist.ac.at)
  • When the bacteria lost their ability to infect, the number of males and females equalized. (icr.org)
  • Wolbachia are maternally inherited endosymbionts that infect nearly half of all arthropod species. (nature.com)
  • It can be made to infect human tissue culture cells in the laboratory but these laboratory systems are very artificial and do not predict the actual ability of Wolbachia to infect an actual human being. (real-agenda.com)
  • Once Wolbachia infect a host arthropod, it wraps itself in a layer of the arthropod's membrane. (phys.org)
  • Specifically, he and his lab members study endosymbiotic Wolbachia bacteria, which infect about half the insects on the planet. (umt.edu)
  • These results show that Wolbachia has transferred horizontally to infect multiple female lineages during the early spread through L. clavipes. (datadryad.org)
  • The different Wolbachia strains in Culex will make the male and female mosquitos produce infertile eggs. (hawaiifreepress.com)
  • Additionally, bidirectional CI can occur if the male and female are infected with different Wolbachia strains and has been implicated as a cause of host speciation and is also being exploited as a method for biocontrol of insect disease vectors. (uu.se)
  • I am currently involved in several genome sequencing projects of Wolbachia strains infecting different Drosophila species with the aim of determining the evolutionary mechanisms involved inshaping their genomes and determining the genetic factors in phenotypic expression. (uu.se)
  • The degree of virus inhibition varies between Wolbachia strains. (bvsalud.org)
  • aegypti lines carrying strains wAu and wAlbA: with the hypothesis that differences in perturbations may underline Wolbachia-mediated antiviral activity. (bvsalud.org)
  • Together these results suggest that wAu could show unique features in its inhibition of arboviruses compared to previously characterized Wolbachia strains. (bvsalud.org)
  • Strains of O volvulus that carry Wolbachia DNA are associated with a higher incidence of ocular disease. (medscape.com)
  • Although studies have reported Wolbachia strains in the An. (cdc.gov)
  • Molecular confirmation of mosquito species and Wolbachia multilocus sequence typing (MLST) were carried out to analyse phylogenetic relationships of mosquito hosts and newly discovered Wolbachia strains. (cdc.gov)
  • Wolbachia are gram-negative bacteria that form intracellular inherited infections in many invertebrates. (gnxp.com)
  • All members are intracellular endosymbionts of invertebrates, and it has been estimated that as many as 15-70% of all insect species on earth are infected by Wolbachia . (uu.se)
  • Matthew Aliota, a scientist at the UW-Madison School of Veterinary Medicine (SVM) and first author of the paper - published today (July 1, 2016) in the journal Scientific Reports - says the bacteria could present a "novel biological control mechanism," aiding efforts to stop the spread of Zika virus. (scienceblog.com)
  • The mosquitoes were either harboring the same strain of the Wolbachia bacteria (called wMel) used in field studies or were Wolbachia -free and the mice had levels of virus in their blood similar to humans infected with Zika virus. (scienceblog.com)
  • An additional group of mosquitoes, both wild-type and Wolbachia -infected, was allowed to feed instead from a membrane containing sheep's blood spiked with a high concentration of Zika virus, per other standard laboratory studies. (scienceblog.com)
  • They found that mosquitoes carrying Wolbachia were less likely to become infected with Zika virus after feeding on viral blood, and those that were infected were not capable of transmitting the virus in their saliva. (scienceblog.com)
  • Mosquitoes with Wolbachia were less capable of harboring Zika virus, and though they do get infected with Zika, it is to a lesser extent than wild-type mosquitoes. (scienceblog.com)
  • The w AlbB strain of Wolbachia is also effective for blocking transmission of other viruses including Zika, chikungunya, and yellow fever. (gla.ac.uk)
  • Although portable tools and "biopesticides" such as Wolbachia are currently effective, adaptive dengue and Zika viruses will likely evolve past these tactics just as they have for countless other methods. (mongabay.com)
  • The first mosquitoes carrying Wolbachia, bacteria that prevent them from transmitting arboviruses (including dengue, Zika and chikungunya), were released on. (pasteur.fr)
  • This capability could be used to enhance ongoing efforts that use Wolbachia to fight dengue fever and Zika virus. (phys.org)
  • The ability to genetically engineer Wolbachia could lead to inserting genes that cause the bacteria to produce traits that increase the effectiveness of usingWolbachia against dengue and Zika viruses. (phys.org)
  • Researchers in Australia are attempting to breed mosquitoes and injecting their eggs with Wolbachia bacteria, which inhibit Zika, dengue fever, and other types of flavivirus. (courthousenews.com)
  • Wolbachia bacteria inhibit dengue virus replication by hijacking gene expression in mosquitoes, Australian researchers have found. (abc.net.au)
  • Using a strain of the bacteria Wolbachia , which inhibit mosquitoes from transmitting viruses to humans, researchers at the Universities of Glasgow and Melbourne and the Institute for Medical Research in Malaysia were successfully able to reduce cases of dengue at sites in Kuala Lumpur. (gla.ac.uk)
  • These include Release of Insects Carrying a Dominant Lethal system and Wolbachia , an endosymbiotic bacterium, to inhibit dengue virus in the vector. (who.int)
  • Using a murine model for river blindness in which soluble extracts of filarial nematodes were injected into the corneal stroma, we demonstrated that the predominant inflammatory response in the cornea was due to species of endosymbiotic Wolbachia bacteria. (qxmd.com)
  • A recent study in three black fly species in the genus Simulium found differential Wolbachia prevalence among species, suggesting host-specific interactions 16 . (nature.com)
  • That is one of the surprise discoveries made by a pair of Vanderbilt biologists when they sequenced the genome of a virus that attacks Wolbachia, a bacterial parasite that has successfully infected not only black widow spiders but more than half of all arthropod species, which include insects, spiders and crustaceans. (phys.org)
  • The aim of this study was to determine the Wolbachia infection prevalence among Drosophila species that are common in Ukraine. (ub.edu)
  • The total of 203 imago, representatives of seven Drosophila species collected from seven localities in Ukraine were screened for Wolbachia via PCR assay. (ub.edu)
  • In Ukraine, the examined Drosophila species are characterised by a low prevalence of Wolbachia infection. (ub.edu)
  • Despite being so common, Wolbachia prevalence varies through time and space in many host species. (umt.edu)
  • It's pretty surprising to see a bacterial species living inside the mitochondrion, which itself was a bacterium," he says. (fightaging.org)
  • Wolbachia is a bacterial genus consisting of only one recognized species, Wolbachia pipientis , with several divergent supergroups. (uu.se)
  • The abundance of bacteria from the Enterobacteriaceae family increased with CHIKV infection whereas the abundance of known insect endosymbionts like Wolbachia and Blattabacterium decreased. (datadryad.org)
  • Our broader research goal is to understand why Wolbachia are the most common endosymbionts in nature. (umt.edu)
  • O volvulus endosymbionts, such as the Wolbachia bacteria that are released upon its death, also cause an immunogenic response. (medscape.com)
  • Wolbachia ( w Bm) is an obligate endosymbiotic bacterium of Brugia malayi , a parasitic filarial nematode of humans and one of the causative agents of lymphatic filariasis. (biomedcentral.com)
  • Instead of working to eliminate mosquitoes the program encourages the release of mosquitoes infected with the bacteria Wolbachia, which interrupts the transmission of dengue. (mystateline.com)
  • A study publishing on 30th May 2017 in the open access journal PLOS Biology by Michael Turelli from University of California, Davis in collaboration with Nick Barton from the Institute of Science and Technology (IST Austria) and colleagues from Scott O'Neill's "Eliminate Dengue Program" demonstrates that over time, strategic releases may allow mosquitoes infected with the dengue-suppressing bacteria to spread across large cities. (ist.ac.at)
  • The technique utilizes mosquitoes infected in a lab with the naturally occurring Wolbachia bacteria, which affects insect reproduction. (hawaiifreepress.com)
  • This toolkit can also determine whether a mosquito has been exposed to the Wolbachia bacteria, which infects mosquitoes and prevents them from transmitting dangerous pathogens. (mongabay.com)
  • When Wolbachia infects mosquitoes, the mosquitoes' ability to transmit Dengue virus is almost completely blocked. (real-agenda.com)
  • In Australian Eurema hecabe butterflies Wolbachia feminises genetic males and convert them into functional females (collaboration with Darrell Kemp at Macquarie University). (exeter.ac.uk)
  • Data -- Clonal genetic variation in a Wolbachia-infected asexual wasp: horizontal transmission or historical sex? (datadryad.org)
  • As of yet, the underlying molecular mechanism of CI and the genetic factors of Wolbachia that are involved are unknown. (uu.se)
  • The birth control method uses male mosquitoes (which do not bite) with a strain of a bacterium called Wolbachia that is incompatible with the strain of Wolbachia currently found in wild mosquitoes in Hawaiʻi. (hawaiifreepress.com)
  • According to Birds, Not Mosquitoes, "We need these Wolbachia-incompatible male mosquitoes to be listed so that importation can commence. (hawaiifreepress.com)
  • We also study endosymbiotic bacteria that live in eukaryotic cellsand often manipulate their hosts by causing changes to the ubiquitin system. (yale.edu)
  • Wolbachia can be found in up to 60 percent of insects around the world, including butterflies and bees. (scienceblog.com)
  • In our research Wolbachia-infected insects are feeding on our researchers all the time and there is no sign of any human illness associated with insect Wolbachia. (real-agenda.com)
  • The infected pests are unable to produce viable offspring with either unafflicted insects or those with a different strain of the bacteria. (hawaiifreepress.com)
  • Wolbachia is an insect bacterium that has not been detected living inside humans or any other vertebrates. (real-agenda.com)
  • Wolbachia manipulate their hosts to maximize their transmission, but they can also provide benefits such as nutrients and resistance against viruses to their hosts. (nature.com)
  • Christoph Bleidorn is working on the evolution of Wolbachia bacteria and their arthropod hosts using genomic approaches. (uni-goettingen.de)
  • When placed inside mosquitoes, Wolbachia variants associated with fly hosts that Cooper studies block arboviruses that cause human diseases such as dengue. (umt.edu)
  • A third, more recent research focus has been on endosymbiotic bacteria that manipulate their eukaryotic hosts by secreting enzymes, including ubiquitin-specific proteases, into the host cell cytoplasm. (yale.edu)
  • Wolbachia are endocellular bacteria known for manipulating the reproductive systems of many of their invertebrate hosts. (datadryad.org)
  • For instance, Wolbachia modulates the replication of viruses or parasites. (datadryad.org)
  • Importantly, because Wolbachia limits, but does not completely prevent, replication of ZIKV and other viruses in coinfected mosquitoes, there is a possibility that these viruses could evolve resistance to pathogen blocking. (bvsalud.org)
  • No genes are modified in the mosquito or in the Wolbachia bacteria. (hawaiifreepress.com)
  • The proposed technique does not modify the genes of mosquitoes or Wolbachia. (hawaiifreepress.com)
  • Drosophila pseudoobscura (X-linked meiotic driver genes causing female biased sex ratios) and D. melanogaster and D. simulans (the cytoplasmic maternally inherited bacterium Wolbachia causing reduced hatching success, CI). (exeter.ac.uk)
  • In contrast with previous studies, RNA was extracted from adult females (n = 516) to increase the chances for the detection of actively expressed Wolbachia genes, determine Wolbachia prevalence rates and estimate relative strain densities. (cdc.gov)
  • The Wolbachia strain w Mel was recently found to increase locomotor activities and possibly trigger cytoplasmic incompatibility in the transinfected fly Drosophila nigrosparsa . (nature.com)
  • We found Wolbachia infection only in one individual of Drosophila testacea that was collected in the Chornobyl Exclusion Zone. (ub.edu)
  • These range from classical mutualism to reproductive parasitism as characterized by the ability of Wolbachia to override chromosomal sex determination, induce parthenogenesis, selectively kill males, influence sperm competition and generate cytoplasmic incompatibility in early embryos. (gnxp.com)
  • aegypti infected with the wMel strain of Wolbachia that are being released by the World Mosquito Program in Medellín, Colombia. (bvsalud.org)
  • Using the Wolbachia Bacterial Symbiont to Teach Inquiry-Based Science: A High School Laboratory Series. (mbl.edu)
  • For example, a maternally inherited symbiont bacteria, e.g. (bvsalud.org)
  • He and Senior Research Specialist Sarah Bordenstein reported the results of their study in a paper titled "Eukaryotic association module in phage WO genomes from Wolbachia" published Oct. 11 in the journal Nature Communications . (phys.org)
  • We assessed variance of markers on the nuclear, mitochondrial and Wolbachia genomes. (datadryad.org)
  • The infection is transferred maternally from mother to offspring and Wolbachia has evolved at least four different ways to manipulate the host reproduction in order to increase the number of infected females in a population. (uu.se)
  • Much of the success of Wolbachia can be attributed to the diverse phenotypes that result from infection. (gnxp.com)
  • The unique biology of Wolbachia has attracted a growing number of researchers interested in questions ranging from the evolutionary implications of infection through to the use of this agent for pest and disease control. (gnxp.com)
  • The infected females hereby achieve a reproductive advantage over the uninfected females in a population and CI can thus result in spread of the Wolbachia infection throughout a population that was previously uninfected. (uu.se)
  • Comparative analyses using ZIKV-infected, uninfected tissues, and mosquitoes without Wolbachia revealed that the influence of wMel on mosquito gene transcription is multifactorial. (bvsalud.org)
  • Surprisingly, wAu-carrying midguts not only showed distinct proteome perturbations when compared to non-Wolbachia carrying and wAlbA-carrying midguts but also wMel-carrying midguts. (bvsalud.org)
  • The researchers found that Wolbachia-infected mosquitoes spread at about 100-200 meters per year in the larger sites, but there was little evidence of such spread in the smaller area even after two years. (ist.ac.at)
  • Researchers led by Jorge Osorio, a UW-Madison professor of pathobiological sciences, and Scott O'Neill of the the Eliminate Dengue Program (EDP) and Monash University in Melbourne, Australia, are already releasing mosquitoes harboring the Wolbachia bacterium in pilot studies in Colombia, Brazil, Australia, Vietnam and Indonesia to help control the spread of dengue virus. (scienceblog.com)
  • Now, this international team of researchers has shown that the wAlbB strain of Wolbachia is stable and effective, even in daily peak temperatures of 36 degrees and higher, as commonly experienced in areas of Malaysia where dengue is prevalent. (gla.ac.uk)
  • The small dots in the bacterium are WO phage particles. (phys.org)
  • Discovering DNA related to the black widow spider toxin gene came as a total surprise because it is the first time that a phage - a virus that infects bacteria - has been found carrying animal-like DNA," said Associate Professor of Biological Sciences Seth Bordenstein. (phys.org)
  • We suspect it makes pores in the membranes of the arthropod cells that surround Wolbachia, thereby allowing the phage to overcome both the bacterial and arthropod membranes that surround it. (phys.org)
  • The large dark patch is bacteria DNA that has been degraded by the phage. (phys.org)
  • The image also shows the multiple wasp membranes wrapped around the Wolbachia that the phage must penetrate both to enter and escape from the bacteria. (phys.org)
  • They correlated this phenomenon with the activity of the bacterial parasite Wolbachia , which infects only the males. (icr.org)
  • Use of the bacterial parasite has two potential advantages compared to other approaches: It doesn't rely on toxic chemicals and, once it is introduced, the bacteria spread rapidly through the mosquito population and sustain themselves. (phys.org)
  • Gene Expression: What can Wolbachia teach us about the Christian Right? (gnxp.com)
  • I am also working on the effect of Wolbachia on the host gene expression by using RNAseq. (uu.se)
  • Wolbachia, a widespread bacterium which can influence mosquito-borne pathogen transmission, has recently been detected within Anopheles (An. (cdc.gov)
  • Vector-associated bacteria have recently been found to interact with transmitted pathogens. (datadryad.org)
  • What's harder to see are climate change's effects on the spread of disease: on the mosquito that carries a virus, or the pathogenic bacteria on a piece of fruit. (cfr.org)
  • Por ejemplo, una bacteria simbionte heredada por vía materna, como puede ser WOLBACHIA, induce cambios en los espermatozoides del huésped, causando defectos mitóticos en el embrión fertilizado solo en cruces entre machos y hembras simbiónticos portadores de un simbionte diferente o de ningún simbionte. (bvsalud.org)
  • Previously, scientists have carried out successful mosquito releases using a different strain of the Wolbachia bacteria, but while this strain was effective in some conditions, it did not appear to be suitable for use in the very hot conditions experienced in equatorial countries such as Malaysia. (gla.ac.uk)
  • It's important to note that Wolbachia does not pose any threat to humans-it solely impacts the mosquitoes, making it an environmentally friendly approach that preserves the delicate balance of our ecosystems. (expresshealthcaremgmt.com)
  • Q: Can humans be infected with Wolbachia bacteria? (expresshealthcaremgmt.com)
  • A: Wolbachia renders mosquitoes that carry the dengue virus sterile, preventing them from transmitting the virus when they bite humans. (expresshealthcaremgmt.com)
  • The scientific community has expressed concern about introducing a new type of mosquito that is infected with a bacterium that could be transmitted to humans. (real-agenda.com)
  • Mosquitos infected with the bacteria Wolbachia are significantly worse vectors for dengue virus, and are therefore a promising tool in fighting the disease, but how to establish and spread Wolbachia in an urban mosquito population is unclear. (ist.ac.at)
  • Prior to the Loa loa study, the leading hypothesis was that Wolbachia offers metabolic support to the worms, providing some essential nutrient. (broadinstitute.org)
  • The oval shape in this electron microphotograph is a Wolbachia bacterium that has infected a Nasonia wasp. (phys.org)
  • This electron microphotograph shows two Wolbachia cells that have infected a Nasonia wasp. (phys.org)
  • The nuclear and mitochondrial markers displayed significant and congruent variation among thelytokous wasp lineages, showing that multiple lineages have become infected with Wolbachia. (datadryad.org)
  • While the bacteria were active, one population contained ten times more females than males. (icr.org)