• H2O2
  • In these investigations, you will combine catalase and H2O2 under various conditions of enzyme and substrate concentration, pH, ionic concentration, and temperature. (biology-online.org)
  • While the complete mechanism of catalase is not currently known, the reaction is believed to occur in two stages: H2O2 + Fe(III)-E → H2O + O=Fe(IV)-E(.+) H2O2 + O=Fe(IV)-E(.+) → H2O + Fe(III)-E + O2 Here Fe()-E represents the iron center of the heme group attached to the enzyme. (wikipedia.org)
  • This enzyme catalyses the following chemical reaction (1) donor + H2O2 ⇌ {\displaystyle \rightleftharpoons } oxidized donor + 2 H2O (2) 2 H2O2 ⇌ {\displaystyle \rightleftharpoons } O2 + 2 H2O This enzyme is a strong catalase with H2O2 as donor which releases O2. (wikipedia.org)
  • mammalian
  • 1972 a) enabled the identification of catalase-positive particles (CPs) in almost all the mammalian cell types investigated thus far. (springer.com)
  • Catalase is ubiquitously expressed in mammalian and non-mammalian aerobic cells containing the cytochrome system. (celltechnology.com)
  • bacteria
  • Catalase that is released by the bacteria plays a role in protecting the bacteria from being destroyed by white blood cells during an infection. (biology-online.org)
  • genes
  • The catalase gene is adjacent to a large region of the genome that appears to be far less compact than is typical of microsporidian genomes, a characteristic which may make this region more amenable to the insertion of foreign genes. (mendeley.com)
  • Found
  • The catalase found here can act on toxic molecules that are transported to the periplasm or the enzyme can be released outside the bacterial wall where it can act on toxic molecules in the environment. (biology-online.org)
  • In 1900, Oscar Loew was the first to give it the name catalase, and found it in many plants and animals. (wikipedia.org)
  • cells
  • This type of catalase is a particularly robust enzyme that has been shown to function in dormant cells, indicating that the N. locustae catalase may play some functional role in the spore. (mendeley.com)
  • reaction
  • The reaction contained 20uM H202 (final) per well and the indicated amounts of catalase in 1X reaction Buffer. (celltechnology.com)
  • The optimum pH for human catalase is approximately 7, and has a fairly broad maximum: the rate of reaction does not change appreciably between pH 6.8 and 7.5. (wikipedia.org)