Caspases. Medical search. Frequent questions

Caspases (cysteine-aspartic proteases, cysteine aspartases or cysteine-dependent aspartate-directed proteases) are a family of protease enzymes playing essential roles in programmed cell death. (wikipedia.org)
Caspases are a family of calcium -dependent cysteine proteases , which play essential roles in apoptosis (programmed cell death), necrosis and inflammation . (wikidoc.org)
They are called cysteine proteases , because they use a cysteine residue to cut those proteins, and are called caspases because the cysteine residue cleaves their substrate proteins at specific asparagine residues. (wikidoc.org)
Unlike the clan CA cysteine proteases, the catalytic histidine in caspase-3 plays a critical role during protonation and subsequent ring opening of the epoxide moiety and facilitates the nucleophilic attack by the active site cysteine. (rcsb.org)
Caspase-8 (FLICE/MACH-1) is a 55 kDa cytosolic protein with homology to the CD95/Fas-associated signal transducer, FADD/MORT-1, as well as to other caspase (ICE/Ced-3) cysteine proteases. (bdbiosciences.com)
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. (neuromics.com)
The caspases are a family of cysteine proteases that induce apoptosis in response to a variety of intra- and extracellular stimuli. (southernbiotech.com)
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. (sigmaaldrich.com)
The process of apoptosis is executed by a family of cysteine proteases called caspases. (duke.edu)
Caspases are a family of proteases that have central functions in programmed cell death (apoptosis) and inflammation. (biomedcentral.com)

The exact contribution that the cleavage of many caspase substrates makes to the biochemistry and morphology of apoptosis is unclear. (wikidoc.org)
CellEvent Caspase-3/7 Green and CellEvent Caspase-3/7 Red detection reagents are novel fluorogenic substrates for activated caspase-3/7 in live cells that can be fixed and multiplexed with antibody detection workflows such as immunofluorescence (IF) and immunocytochemistry (ICC). (thermofisher.com)
pNA (4-nitroaniline)-derived caspase substrates are widely used for the colorimetric detection of various caspase activities. (eurogentec.com)
Active caspase identifies multiple molecules as substrates and cleaves them, leading to biological events associated with apoptosis. (caltagmedsystems.co.uk)
Caspase also recognises several structural proteins as substrates, leading to cleavage that is associated with distinct morphological changes in apoptotic cells, including chromatin condensation, nucleus fragmentation, and cytoplasmic integrity. (caltagmedsystems.co.uk)
Caspases mediate their effects through aspartate-specific cleavage of their target proteins, and at present almost 400 caspase substrates are known. (biomedcentral.com)

They are named caspases due to their specific cysteine protease activity - a cysteine in its active site nucleophilically attacks and cleaves a target protein only after an aspartic acid residue. (wikipedia.org)
Horvitz and his colleague Junying Yuan [2] found in 1993 that the protein encoded by the ced-3 gene was a cysteine protease with similar properties to the mammalian interleukin-1-beta converting enzyme (ICE) (now known as caspase 1) which at the time was the only known caspase. (wikidoc.org)
Caspase belongs to the cysteine aspartic acid-specific protease family, which gets activated by various signals such as death receptor ligation, DNA damage, serum starvation, and stresses. (caltagmedsystems.co.uk)

The activation of initiator caspases and inflammatory caspases is initiated by dimerisation, which is facilitated by binding to adaptor proteins via protein-protein interaction motifs that are collectively referred to as death folds. (wikipedia.org)
The pro-domain of the intrinsic initiator caspases and the inflammatory caspases contains a single death fold known as caspase recruitment domain (CARD), while the pro-domain of the extrinsic initiator caspases contains two death folds known as death effector domains (DED). (wikipedia.org)
Initiator caspases (e.g. (wikidoc.org)
Initiator caspases possess a longer prodomain than the effector caspases, whose prodomain is very small. (wikidoc.org)
The prodomain of the initiator caspases contain domains such as a CARD domain (e.g. caspases-2 and -9) or a death effector domain (DED) (caspases-8 and -10) that enables the caspases to interact with other molecules that regulate their activation. (wikidoc.org)
These molecules respond to stimuli which cause the clustering of the initiator caspases. (wikidoc.org)
Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase-9. (neuromics.com)
Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse. (neuromics.com)

Tumour growth can occur by a combination of factors, including a mutation in a cell cycle gene which removes the restraints on cell growth, combined with mutations in apoptotic proteins such as caspases that would respond by inducing cell death in abnormally growing cells. (wikipedia.org)
Caspases are essential in cells for apoptosis , one of the main types of programmed cell death in development and most other stages of adult life, and have been termed "executioner" proteins for their roles in the cell. (wikidoc.org)
Viral proteins (v-FLIPS) which inhibit recruitment and activation of caspase-8 have been isolated. (bdbiosciences.com)
Activation of this enzyme can occur via the interaction of its caspase recruitment domain with CARD SIGNALING ADAPTOR PROTEINS. (umassmed.edu)
There are several methods developed to predict caspase cleavage sites from individual proteins, but currently none of them can be used to predict caspase cleavage sites from multiple proteins or entire proteomes, or to use several classifiers in combination. (biomedcentral.com)
A database was constructed with the developed tool, and it was used to identify caspase target proteins from tandem mass spectrometry data from two different proteomic experiments. (biomedcentral.com)
We have developed Pripper, a tool for reading an arbitrary number of proteins in FASTA format, predicting their caspase cleavage sites and outputting the cleaved sequences to a new FASTA format sequence file. (biomedcentral.com)
We show that Pripper is a valuable tool in identifying novel caspase target proteins from modern proteomics experiments. (biomedcentral.com)
Caspases mediate their effects through aspartate-specific cleavage of their target proteins, and several hundreds of caspase target proteins have been identified [ 3 ]. (biomedcentral.com)
Studies of amino acids in the motif have revealed that the motif is only moderately conserved between target proteins of different caspases. (biomedcentral.com)
The prediction of caspase cleavage sites from proteins is not a trivial task due to the heterogeneity of target sequences, and several different tools have been developed to predict caspase cleavage sites from individual proteins. (biomedcentral.com)

Activation involves dimerization and often oligomerisation of pro-caspases, followed by cleavage into a small subunit and large subunit. (wikipedia.org)
The large and small subunit associate with each other to form an active heterodimer caspase. (wikipedia.org)
Some activating multiprotein complexes includes: The death-inducing signaling complex (DISC) during extrinsic apoptosis The apoptosome during intrinsic apoptosis The inflammasome during pyroptosis Once appropriately dimerised, the Caspases cleave at inter domain linker regions, forming a large and small subunit. (wikipedia.org)
They are first synthesized as inactive pro-caspases , that consist of a prodomain, a small subunit and a large subunit. (wikidoc.org)
Whereas only partial caspase-3A encoding sequences were isolated, the full-length caspase-3B cDNA encodes the inactive proenzyme of 279 amino acids (aa) consisting of an N-terminal prodomain and the large and the small subunit. (nofima.no)
The deduced salmon caspase-7 consists of only 245 aa and lacks the prodomain and part of the large subunit similar to the predicted caspase-7 of the puffer fish Tetraodon sp. (nofima.no)

Promotes apoptosome formation and activates caspase-9/caspase-3 pathway. (rndsystems.com)
Nuclear factor-kappa B (NF- κ B) inhibits cell death through suppression of the caspase cascade, the c-Jun N-terminal kinase (JNK) pathway, and reactive oxygen species (ROS) accumulation. (nature.com)
Here we demonstrate that, during brain maturation, the potential of the intrinsic apoptotic pathway is progressively reduced and that such repression is associated with downregulation of apoptotic protease-activating factor-1 (Apaf-1) and caspase-3 gene expression. (jneurosci.org)
These results suggest that differential expression of Apaf-1 and caspase-3 genes may underlie regulation of apoptotic susceptibility during brain development, as well as after acute injury to mature brain, through the intrinsic pathway of caspase activation. (jneurosci.org)
Eldadah and Faden, 2000 ), we hypothesized that suppression of apoptotic capability during maturation of mammalian brain results from repression of genes involved in the caspase-3 activation pathway, and that injury-induced neuronal apoptosis in the mature brain results from reactivation of these genes. (jneurosci.org)
Caspase-2, an apoptotic initiator, can be suppressed by high levels of nutrient flux through the pentose phosphate pathway (PPP). (duke.edu)
These results suggest that chemotherapeutic drug-induced caspase activation is entirely controlled by a receptor-independent mitochondrial pathway, whereas CD95-induced apoptosis can be regulated by a separate pathway not requiring Apaf-1 function. (diva-portal.org)
Finally, the function of CED-3 caspase in this context is dependent on its activation through the conserved egl-1 BH3-only, ced-9 Bcl-2, and ced-4 Apaf-1 pathway. (ucl.ac.uk)
This caspase‐3 activation concurs with a rapid loss of dendritic spines and deficits in synaptic long‐term depression (LTD) in striatal projection neurons forming the indirect pathway. (lu.se)
Interestingly, systemic treatment with a caspase inhibitor prevents both the spine pruning and the deficit of indirect pathway LTD without interfering with the ongoing dopaminergic degeneration. (lu.se)
Taken together, our data identify transient and non‐apoptotic caspase activation as a critical event in the early plastic changes of indirect pathway neurons following dopamine denervation. (lu.se)
This pathway seems to be triggered by depletion of NAD (+) and appears to be caspase independent. (bvsalud.org)
Gentamicin-induced hair cell death was initiated through the caspase-9 intrinsic apoptotic pathway followed by activation of downstream executioner caspase-3. (cdc.gov)
Support cell death was initiated through the caspase-8 extrinsic apoptotic pathway followed later by downstream activation of caspase-3. (cdc.gov)

MCF-7) and this phenotype is blocked by overexpression of the caspase-3 protease inhibitor, CrmA. (bdbiosciences.com)
Caspase-8 is a cytosolic protease that is capable of processing/activating all known caspases, indicating its presence early in the apoptotic cascade. (southernbiotech.com)
In this webinar, learn about the mechanisms of pyroptosis, including the critical role of caspase-1, a protease involved in activation of pro-inflammatory cytokines, plasma membrane rupture, and other morphological characteristics of pyroptosis. (immunochemistry.com)
The nonapoptotic caspase function of both caspase-8 and -10 does not require the protease activity and the DED-containing prodomains are sufficient for NF-kB activation. (reactome.org)
The cone-enriched apoptotic protease caspase-7 ( Casp7 ) is thought to be triggered by endoplasmic reticulum (ER) stress and plays a pivotal role in mice deficient in the cone cyclic nucleotide-gated channels, a deficiency that causes achromatopsia in humans and in mice with autosomal dominant rhodopsin mutations, in particular the T17M mutation. (molvis.org)
In all cases, apoptosis is mediated by caspases, although it is unclear how these diverse apoptotic stimuli cause protease activation. (diva-portal.org)
Pripper is not restricted to predicting only caspase cut sites, but it gives the possibility to scan protein sequences for any given motif(s) and predict cut sites once a suitable cut site prediction model for any other protease has been developed. (biomedcentral.com)

the apoptosome (regulated by cytochrome c and the Bcl-2 family ) which activates caspase-9 . (wikidoc.org)
Active caspase-9, in turn, activates executioner caspases-3 and -7. (jneurosci.org)
For instance, caspase-3 indirectly activates CAD (caspase-activated deoxyribonuclease) while inactivating ICAD (inhibitor of caspase-activated deoxyribonuclease), which contributes to chromatin fragmentation in nucleosome units. (caltagmedsystems.co.uk)
Endoplasmic Reticulum Stress Activates the Inflammasome via NLRP3- and Caspase-2-Driven Mitochondrial Damage. (umassmed.edu)

Full-length recombinant human caspase-8 protein was used as immunogen. (bdbiosciences.com)
Full-length recombinant human Caspase 7 protein. (neuromics.com)
Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining substrate specificity, and as a positive control in caspase activity assays. (enzolifesciences.com)

Caspase-3 cleaves the death substrate PARP to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. (thomassci.com)
MBLI 's APOPCYTO Caspase-3,8,9 Colorimetric Assay Kit detects caspase activity in cell extract with LEHD-pNA as a substrate, in which LEHD sequence is recognised by each active caspase selectively. (caltagmedsystems.co.uk)
The Caspase-8 substrate and pNA standard are especially light sensitive. (sigmaaldrich.com)
Ac-LEHD-AFC is a fluorogenic substrate for caspase-4, caspase-5, and caspase-9. (adooq.com)

Caspase recruitment domain-containing protein (Card)9 is a nonredundant adapter protein that functions in the innate immune system in the assembly of multifunctional signaling complexes. (nih.gov)
We have recently shown that tumor necrosis factor (TNF) α induces caspase-dependent and -independent JNK activation and ROS accumulation in cellular FLICE-inhibitory protein (c-Flip) −/− murine embryonic fibroblasts (MEFs). (nature.com)
Injury-induced cytochrome c -specific cleavage of caspase-9 followed by activation of caspase-3 in mature brain correlated with marked increases in Apaf-1 and caspase-3 mRNA and protein expression. (jneurosci.org)
The 2-2.5 fold increase in the mRNA expression of the heat shock protein Hsp70 gene coincided with the peak mRNA values of the executioner caspases. (nofima.no)
This metabolic suppression of caspase-2 is exerted via the inhibitory phosphorylation of S135 on the caspase-2 prodomain by activated Ca2+/Calmodulin-dependent protein kinase II (CaMKII). (duke.edu)
Three different pattern recognition classifiers were developed for predicting caspase cleavage sites from protein sequences. (biomedcentral.com)

CASP2 , CASP8 , CASP9 and CASP10 ) cleave inactive pro-forms of effector caspases, thereby activating them. (wikidoc.org)
This allows them to autoactivate, so that they can then proceed to activate the effector caspases. (wikidoc.org)

We recommend using 1U per assay for analyzing caspase activity. (enzolifesciences.com)

Note that in addition to apoptosis, caspase-8 is also required for the inhibition of another form of programmed cell death called necroptosis. (wikipedia.org)
The five crystal structures of caspase-3-aza-peptide epoxide inhibitor complexes reported here reveal the structural basis for the mechanism of inhibition and the specificities at the S1' and the S4 subsites. (rcsb.org)
In contrast, inhibition of ATP production did not affect caspase activation after triggering of CD95. (diva-portal.org)
We found that glioma-induced microglia conversion was coupled to a reduction in the basal activity of microglial caspase-3 and increased S-nitrosylation of mitochondria-associated caspase-3 through inhibition of thioredoxin-2 activity, and that inhibition of caspase-3 regulated microglial tumor-supporting function. (cam.ac.uk)
[ 8 ] Caspase inhibition in clonal striatal cells showed no correlation between the reduction of aggregates in the cells and increased survival. (medscape.com)

The initiation of this cascade reaction is regulated by caspase inhibitors. (wikidoc.org)
2CNL: Crystal structures of caspase-3 in complex with aza-peptide epoxide inhibitors. (rcsb.org)
A favorable network of hydrogen bonds involving the oxyanion hole, catalytic histidine, and the atoms in the prime site of the inhibitor enhance the binding affinity and specificity of the aza-peptide epoxide inhibitors toward caspase-3. (rcsb.org)

Caspase-3 is a widely expressed dimeric peptidase that is the major executioner caspase, the primary downstream mediator of apoptotic-associated proteolysis. (rndsystems.com)

The active enzyme often exists as a heterotetramer in the biological environment, where a pro-caspase dimer is cleaved together to form a heterotetramer. (wikipedia.org)
However, the function of ICAD/DFF45 is to restrain the enzyme CAD (Caspase Activated DNase). (wikidoc.org)
VX-765 is a novel Caspase-1 inhibitor,which is an enzyme that controls the generation of two cytokines, IL-1b and IL-18. (adooq.com)

Both known caspase cleavage products as well as novel cleavage products were identified using the database demonstrating the usefulness of the tool. (biomedcentral.com)

The antibody recognizes both the proform of human caspase-8 (55/50 kDa doublet) as well as the cleaved form (40/36 kDa doublet) on SDS/PAGE. (bdbiosciences.com)
Purified Anti-Human Caspase-8 antibody for use in western blot assays. (southernbiotech.com)
The SB125a monoclonal antibody detects a band at ~55kD corresponding to the full length form of caspase-8. (southernbiotech.com)
Description: A polyclonal antibody for detection of Caspase-1 from Human, Mouse, Rat. (bd-ibr.org)
This Caspase-1 antibody is for WB, IHC-P, ELISA. (bd-ibr.org)

Following this discovery, the other mammalian caspases, in addition to caspases in other organisms such as the fruit fly Drosophila melanogaster , were soon identified and characterised. (wikidoc.org)
We speculate that the new nonapoptotic function of C. elegans CED-3 caspase in asymmetric neuroblast division is relevant to the function(s) of mammalian caspases in stem cells. (ucl.ac.uk)

The mechanism of 6‑OAP cytotoxicity in all cell lines was associated with the induction of apoptosis with the presence of cleaved caspase‑3 and PARP. (spandidos-publications.com)

Active Caspase-3 contains two p17 and two p12 subunits. (rndsystems.com)

initiator (apical) caspases and effector (executioner) caspases . (wikidoc.org)
To examine the involvement of executioner caspases in hyperthermia-induced cell-death in a poikilotherm vertebrate species, five genes encoding caspase-3,-6, and -7 were cloned from Atlantic salmon, and the expression was studied in thermal stressed salmon embryos. (nofima.no)
Increased apoptotic activity as evidenced by cleavage of nuclear DNA was demonstrated in salmon embryos incubated at 18-20 degrees C for 84h after acclimatization at 8 degrees C. Hyperthermia-induced activation of the executioner caspases was indicated by the increased mRNA levels of caspase-3B, caspase-6A/B and caspase-7 after 54 h heat exposure as quantified by real-time RT-PCR. (nofima.no)
Altogether, these results shed light on evolutionary aspects of the executioner caspases in vertebrates and their expression in salmon embryos exposed to hyperthermia. (nofima.no)

Overexpression of caspase-8 is sufficient to induce apoptosis in certain cell lines (e.g. (bdbiosciences.com)
Our results are consistent with a mechanism in which ligands for the BIR3 domain of XIAP induce apoptosis by freeing up caspases. (rcsb.org)

Caspases also have a role in inflammation, whereby it directly processes pro-inflammatory cytokines such as pro-IL1β. (wikipedia.org)
Given that ROS accumulation and necrotic cell death enhance inflammation followed by compensatory proliferation of tumor cells, selective suppression of caspase-dependent ROS accumulation will be an alternative strategy to protect cells from ROS-dependent DNA damage and compensatory tumor progression. (nature.com)

We'll examine how ImmunoChemistry Technologies' FLICA (Fluorescent-Labeled Inhibitor of Caspases) assays can be used to assess cell death, including caspase-1 activation and pyroptosis. (immunochemistry.com)

Caspases involved with processing inflammatory signals are also implicated in disease. (wikipedia.org)
This is because they are inflammatory caspases, which in concert with CASP1 , are involved in cytokine maturation. (wikidoc.org)
Altogether 15 different caspases have been identified in mammals, and they are grouped into two major subfamilies, inflammatory and apoptotic caspases [ 2 ]. (biomedcentral.com)

Caspase 8 Colorimetric Activity Assay Kit, IETD Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD. (sigmaaldrich.com)

Processing of caspases is required for activation of downstream signaling and dsRNA stimulation inducese the processing of these caspases. (reactome.org)

Kamber Kaya HE, Ditzel M, Meier P, Bergmann A. An inhibitory mono-ubiquitylation of the Drosophila initiator caspase Dronc functions in both apoptotic and non-apoptotic pathways. (umassmed.edu)
A closer look at Drosophila male testes revealed the surprising observation that high levels of caspases are present in wild type testes, along with the caspase activator Ark. (caltech.edu)

Caspase-10 is an initiator caspase, highly homologous to caspase-8, a relationship that extends to the death effector domains (DEDs) of their proforms. (enzolifesciences.com)

Caspases have the ability to cleave after aspartic acid residues. (neuromics.com)

A long pro-domain caspase that contains a caspase recruitment domain in its pro-domain region. (umassmed.edu)

Caspases have other identified roles in programmed cell death such as pyroptosis, necroptosis and PANoptosis. (wikipedia.org)
There are other identified roles of caspases such as cell proliferation, tumour suppression, cell differentiation, neural development and axon guidance and ageing. (wikipedia.org)
Interestingly, dBruce mutant males are sterile, but a lack of increased caspase activity in these mutants suggests that dBruce may also play nonapoptotic roles. (caltech.edu)
It is, however, not known whether caspase‐3 plays similar roles in other pathologies. (lu.se)

Interestingly, cardiac expression of caspase-6AIB was only identified in beat stressed embryos. (nofima.no)
Interestingly, caspase-8, whose function appeared to be restricted to death receptors, was also activated by these drugs under normal conditions, but not after ATP depletion. (diva-portal.org)

Caspases are synthesised as inactive zymogens (pro-caspases) that are only activated following an appropriate stimulus. (wikipedia.org)

These data, combined with the inability of dBruce to block death induced by the apical caspase Dronc or the proapoptotic Bcl-2 family member Debcl/Drob-1/dBorg-1/Dbok, suggest that dBruce regulates cell death at a novel point. (caltech.edu)

Mutations in CRADD Result in Reduced Caspase-2-Mediated Neuronal Apoptosis and Cause Megalencephaly with a Rare Lissencephaly Variant. (umassmed.edu)

Caspase-10 was reported to activate early in TRAIL-induced apoptosis and was found in association with the DR4 (TRAIL-R1) receptor complex in a thyroid carcinoma line. (enzolifesciences.com)

this coupled with the unwanted apoptosis that occurs with ischaemia or Alzheimer's disease , has boomed the interest in caspases as potential therapeutic targets since they were discovered in the mid 1990s. (wikidoc.org)
Non‐apoptotic caspase‐3 activation is critically involved in dendritic spine loss and synaptic dysfunction in Alzheimer's disease. (lu.se)

Evaluation of the classifiers with quality measures indicated that all of the three classifiers performed well in predicting caspase cleavage sites, and when combining different classifiers the accuracy increased further. (biomedcentral.com)

Consistent with the results using c-Flip −/− MEFs, we found that TNF α stimulation induced caspase-dependent prolonged JNK activation and ROS accumulation, followed by apoptotic and necrotic cell death in various tumor cells. (nature.com)
led to a reduction in both microglia recruitment and tumor expansion, whereas depletion of microglial caspase-3 gene promoted tumor growth. (cam.ac.uk)

Whole-mount in situ hybridization of the salmon embryo identified caspase-7 mRNA in the lens exclusively, while caspase-3B and caspase-6A/B were expressed in multiple tissues of exposed and control embryos. (nofima.no)

This caspase-cascade plays a central role in the induction and transduction of apoptotic signals, as well as in the regulation of immunity, cellular proliferation and differentiation [ 2 ]. (biomedcentral.com)

Cleavage of pNA peptides by caspases generates pNA that is monitored colorimetrically at ~405 nm. (eurogentec.com)

In particular, the heat sensitive caspase-6 expression in the embryonic heart is of interest since cardiac malformations are an emergent problem in salmon aquaculture. (nofima.no)
In addition, we provide evidence that a pig-1 MELK (maternal embryonic leucine zipper kinase)-dependent reciprocal gradient of "mitotic potential" is formed in the QL.p neuroblast, and that CED-3 caspase antagonizes this mitotic potential. (ucl.ac.uk)

The detection of caspase activity is crucial for apoptosis research. (caltagmedsystems.co.uk)
Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity. (sigmaaldrich.com)
Furthermore, we identified the activity of nitric oxide synthase 2 (NOS2, also known as iNOS) originating from the glioma cells as a driving stimulus in the control of microglial caspase-3 activity. (cam.ac.uk)
We have previously shown that CED-3 caspase is activated in such neuroblasts, and that before neuroblast division, a gradient of CED-3 caspase activity is formed in a ced-1 MEGF10 (multiple EGF-like domains 10)-dependent manner. (ucl.ac.uk)
The possibility that cytotoxic effects were due to apoptosis was indicated by significant time- and dose-dependent induction of caspase-3/7 activity in both HepG2 cells and human hepatocytes. (cdc.gov)

CellEvent Caspase-3/7 Red Detection Reagent can also be used to detect apoptosis in GFP expressing cells. (thermofisher.com)
APOPCYTO Caspase-3,8,9 Colorimetric Assay Kits are composed of reagents necessary for the detection, which includes Cell Lysis buffer, Reaction buffer and so on. (caltagmedsystems.co.uk)

Anatomy14

Diseases1

Chemicals and Drugs81

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes21

Information Science2

Proteases10

Substrates6

Cysteine3

Initiator caspases8

Proteins11

Subunit6

Pathway14

Protease7

Activates4

Recombinant3

Substrate4

Protein6

Effector caspases2

Analyzing caspase activity1

Inhibition5

Inhibitors3

Major executioner caspase1

Enzyme3

Known caspase1

Antibody5

Mammalian2

PARP1

Active caspase1

Executioner caspases4

Induce apoptosis2

Inflammation2

Assays1

Inflammatory3

Assay Kit1

Activation of downstream1

Drosophila2

Death effector domains1

Cleave1

Recruitment domain1

Roles4

Interestingly2

Synthesised as inactive1

Dronc1

Neuronal apoptosis1

Activate1

Alzheimer's2

Cleavage sites1

Tumor2

MRNA1

Regulation1

Peptides1

Embryonic2

Activity5

Detection2