• Due to their limited fates, they would not be considered pluripotent stem cells, but they still provide a significant number of differentiation paths that can be used for a wide variety of applications. (wikipedia.org)
  • One complementary therapy could be 're-activating' vasculogenesis (i.e. the differentiation of precursor cells into mature endothelial cells), a mechanism that occurs in the heart during development but is quiescent in adult hearts. (elifesciences.org)
  • At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
  • The established dogma has however been challenged by recent findings suggesting that multipotent stem cells have a broader differentiation potential than previously thought. (biomedcentral.com)
  • In this review, we describe the cardiac differentiation from ES cells, iPS cells, and the current progress of using iPS cell-derived cardiomyocytes for heart disease modeling and for the development of therapeutic strategies. (biomedcentral.com)
  • MSCs have multilineage differentiation potential, allowing them to differentiate, for example, into chondrocytes and osteoblasts, the key cells from the two tissues that constitute the osteochondral unit. (hindawi.com)
  • Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
  • Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
  • We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
  • Therefore, researchers have begun exploring combined therapies with biomaterials that promote cell growth and differentiation while localizing cells in the injured area. (karger.com)
  • This article reviews recent advancements in biomaterial strategies for the differentiation of neural stem cells into oligodendrocytes, and presents new data indicating appropriate properties for oligodendrocyte precursor cell growth. (karger.com)
  • Aizawa, Y., N.D. Leipzig, T. Zahira, M.S. Shoichet (2008) The effect of immobilized platelet derived growth factor AA on neural stem/progenitor cell differentiation on cell-adhesive hydrogels. (karger.com)
  • Due to their secretory functions, differentiation capabilities, specific homing effects through chemotaxis, distinctive therapeutic potentials, and ex vivo expandability, cells have become an attractive reagent for advanced therapeutic strategies. (biomedcentral.com)
  • We previously reported that mesenchymal stem cells (MSCs), through local injection upon testicular torsion-detorsion, restored the spermatogenesis without differentiation into sperm. (biomedcentral.com)
  • The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
  • In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
  • Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
  • Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
  • Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
  • These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
  • However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
  • Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
  • culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
  • FAs are structures composed of clustered transmembrane proteins called integrins that bind to the extracellular matrix and link to the actin cytoskeleton and control cell migration, proliferation, survival and differentiation (Gardel et al. (bio2009.org)
  • In 1993-1994 he had the opportunity to work with Ken Chien at the University of California San Diego, where the work on embryonic stem cells started. (umcutrecht.nl)
  • Upon return in The Netherlands he worked with Christine Mummery and Hans Clevers on both human Embryonic as well as human Cardiac stem cells. (umcutrecht.nl)
  • Additionally, it was theorized that stem cells could also be found in the embryonic dorsal aorta, which furthered interest in the subject matter. (wikipedia.org)
  • cloned murine embryonic organs and, after analysis, found cells in the dorsal aorta clones that were able to differentiate into skeletal myogenic progenitors that expressed myogenic markers like MyoD, Myf-5, and desmin. (wikipedia.org)
  • My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
  • Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
  • The cells giving rise to the foetus, are called pluripotent embryonic stem cells, since they can form every cell type in the body. (biomedcentral.com)
  • These cells are commonly termed embryonic germ (EG) cells because they are derived from primordial germ cells found at the genital ridges in developing fetuses. (biomedcentral.com)
  • A recent study indicates that even adult humans might harbor cells resembling pluripotent embryonic stem cells. (biomedcentral.com)
  • and from 2002 on pluripotency in embryonic stem cells in the lab of Ali H. Brivanlou. (eurostemcell.org)
  • He has been leader of a research group at the Max-Delbrück-Center for Molecular Medicine in Berlin until 2011 focusing his research on signal transduction mechanisms in human and murine embryonic stem cells. (eurostemcell.org)
  • During more recent years, his reseach has been conducted in the field of regenerative medicine, focussed on antigen expression and immune recognition of human embryonic stem cells and bioartificial heart valves, the latter tissues being of animal origin. (gu.se)
  • In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
  • Embryonic stem (ES) cells are naturally derived from early stage embryos and induced pluripotent stem (iPS) cells are reprogrammed from somatic cells with overexpression of four reprogramming factors, Oct4, Sox2, Klf4 and c-Myc. (biomedcentral.com)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • An immunologically identical cardiac tissue can be produced by direct reprogramming from the patients' tissue without the potential risk of tumorigenesis that occurs with induced pluripotent stem cells [ 2 ] and the ethical issue using embryonic stem cells. (thno.org)
  • Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
  • Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)
  • Embryonic stem cells are stem cells derived from the inner cell mass of an early, preimplantation stage embryo known as a blastocyst. (bvsalud.org)
  • At this stage, the dorsal aorta consists mainly of an endothelial layer with a few mesenchymal cells on the abluminal side. (wikipedia.org)
  • The origin of new endothelial cells (i.e. resident or infiltrating) as well as the underlying mechanism leading to their proliferation (partial endothelial-to-mesenchymal transition [EndMT] or not) have long been debated. (elifesciences.org)
  • Mesenchymal stem cells (MSCs) from bone marrow play a critical role in osteochondral repair. (hindawi.com)
  • Mesenchymal stem cells (MSCs) play a key role in articular cartilage repair. (hindawi.com)
  • The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. (frontiersin.org)
  • Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
  • In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
  • In particular, stem cells, including mesenchymal stem cells (MSCs), hematopoietic stem cells (HSCs), and induced pluripotent stem cells (iPSCs), and immune cells, such as T-cells and Natural Killer (NK) cells, have been favored candidates for regenerative medicine and cell-based cancer immunotherapy, respectively. (biomedcentral.com)
  • We previously explored that local injection of mesenchymal stem cells (MSCs) 30 min before surgical detorsion was applicable to prevent testicular torsion-induced I/R injury on germ cell and maintain the spermatogenesis at acute stage. (biomedcentral.com)
  • Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. (issca.us)
  • Later, Arnold Caplan's group described mesenchymal stem/stromal cells (MSCs) as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies [2-4]. (issca.us)
  • Pivotal studies by the group of Horwitz in children with osteogenesis imperfecta, an inherited enzyme deficiency of collagen synthesis by mesenchymal cells in bone, opened the field for intravenous use of MSCs. (issca.us)
  • These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)
  • Because of these properties, mesoangioblasts are a precursor of skeletal, smooth, and cardiac muscle cells along with endothelial cells. (wikipedia.org)
  • Mesoangioblasts have the ability to differentiate into multiple cell types, such as skeletal muscle, smooth muscle, and endothelial cells. (wikipedia.org)
  • Mesoangioblast-derived skeletal and cardiac muscle cells expressed TNNT2 and TNNI3, while endothelial cells expressed CD31 and Ve-cadherin, and smooth muscle cells expressed aSMA and smMHC. (wikipedia.org)
  • BNP treatment increased vascularisation and the number of endothelial cells in all areas of infarcted hearts. (elifesciences.org)
  • Endothelial cell lineage tracing showed that BNP directly stimulated the proliferation of resident endothelial cells via NPR-A binding and p38 MAP kinase activation. (elifesciences.org)
  • Our results demonstrated that these immature cells have a natural capacity to differentiate into endothelial cells in infarcted hearts. (elifesciences.org)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • They include endothelial cells, fibroblasts, and keratinocytes. (frontiersin.org)
  • Cardiovascular diseases often cause substantial heart damage and even heart failure due to the limited regenerative capacity of adult cardiomyocytes. (thno.org)
  • Research has suggested their application for stem cell therapies for muscular dystrophy and cardiovascular disease. (wikipedia.org)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • However, for cell therapies MSC have to be expanded and/or manipulated to obtain a sufficient amount of cells that can be subsequently used for treatment. (biomedcentral.com)
  • My research is focused on understanding what stem cell secreted factors are responsible for improved heart function, what their targets are and how these can be modulated to develop new cell-free therapies that can help patients with a wide spectrum of coronary disease and heart failure. (buffalo.edu)
  • Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
  • This explains why researchers are now focusing attention on developing stem cell therapies using postnatal stem cells donated by the patients themselves or their close relatives. (bvsalud.org)
  • BNP also stimulated the proliferation of WT1 + epicardium-derived cells but only in the hypoxic area of infarcted hearts. (elifesciences.org)
  • Overexpression of this gene is associated with tumor cell proliferation and metastasis. (cancerindex.org)
  • Transwell migration, cell cycle analysis, MTT and colony formation assays were performed to determine the effect of DRAM2 overexpression and knockdown on NSCLC-cell migration and proliferation. (cancerindex.org)
  • Additionally, DRAM2 overexpression promoted cell metastasis and proliferation in vitro, while knockdown of DRAM2 expression yielded opposite result. (cancerindex.org)
  • The final result is that, after MI, billions of cardiomyocytes (CMs) with limited proliferation capacity are lost and substituted by heterogeneous collagen-rich fibrotic scar tissue [ 2 ] [ 3 ] . (encyclopedia.pub)
  • Cell Counting Kit 8 (CCK8), 5-ethynyl-2'-deoxyuridine (EDU) assay, cell migration and invasion assay and wound healing measures evaluated the effect of miR-142-3P and FN1 on cell proliferation, migration and invasion. (bvsalud.org)
  • Functionally, miR-142-3P inhibited cell proliferation, migration, invasion and EMT, and induced the cell apoptosis in PTC. (bvsalud.org)
  • Depletion of FN1 rescues the effect of miR-142-3P inhibitor on cell proliferation, invasion, apoptosis and EMT via inactivating Focal Adhesion Kinase (FAK)/Extracellular Signal-Regulated Kinase (ERK) / Phosphoinostide 3-kinase (P13K) signaling. (bvsalud.org)
  • CONCLUSION: miR-142-3P suppressed cell proliferation, migration, invasion and EMT through modulating FN1/FAK/ERK/PI3K signaling in PTC, suggesting it as a potential therapeutic target for PTC. (bvsalud.org)
  • RhoC over-expression has been reported in ductal pancreatic adenosarcoma (Suwa, 1998) and in inflammatory breast cancers (van Golen, 1999). (cancerindex.org)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • It is postulated that the lack of cardiac regeneration in the postnatal heart confounds the myocyte dysfunction, cell death and tissue fibrosis that is associated with decompensated heart failure. (ucdavis.edu)
  • My laboratory focus is in studying the fundamental mechanisms of this growth transition to enhance the translational efficacy of soluble factors (drugs) and cell-based strategies (stem-cells) for cardiac regeneration in the failing heart. (ucdavis.edu)
  • Schwann cells (SCs) have important roles in development, myelination and regeneration in the peripheral nervous system. (scirp.org)
  • The in vivo electrical coupling of the cells leads to a better regeneration of the infarcted myocardium, reducing arrhythmias, QRS/QT intervals, and scar size and promoting cardiac cell maturation. (encyclopedia.pub)
  • however, stem cell injection alone has been unsuccessful in many areas of neural regeneration. (karger.com)
  • Direct cardiac reprogramming of fibroblasts is an attractive therapeutic strategy for treating cardiovascular diseases because the adult heart has a low regeneration capacity [ 1 ]. (thno.org)
  • There are limited numbers of publications about ES cells in pulp regeneration, due to the restricted policies regarding ES cell research over the past few years. (bvsalud.org)
  • In addition, we summarize the recent direct reprogramming of cardiomyocytes from fibroblast cells, which provides another method for potential heart disease therapy. (biomedcentral.com)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • in 1956 and Till and McCulloch in 1961, demonstrating that lethally irradiated mice could be rescued with cells from freshly isolated bone marrow [ 10 ] and that BM cells formed spleen clonies (CFU-S) following transplantation to irradiated recipients [ 30 ], respectively. (biomedcentral.com)
  • The additional application of a bone marrow aspirate (BMA) to the procedure of marrow stimulation is thought to enhance cartilage repair as it may provide both an additional cell population capable of chondrogenesis and a source of growth factors stimulating cartilage repair. (hindawi.com)
  • In the orthopaedic field, additional application of a bone marrow aspirate (BMA) to the procedure of marrow stimulation has been recently studied, since the bone marrow itself is both a source of MSCs, providing a cell population capable of chondrogenesis and of various growth factors stimulating cartilage repair [ 7 - 10 ]. (hindawi.com)
  • A focus is on the steps of mobilization of cells from the subchondral bone and repair tissue formation, including adherence of the bone marrow clot to the subchondral bone. (hindawi.com)
  • In the 1970s, Friedenstein and colleagues [1] first reported that locally applied culture-expanded populations of bone marrow stroma-derived fibroblastic cells remained at their injection sites under the kidney capsule, where an ectopic hematopoiesis was initiated. (issca.us)
  • This concept started from the observation that bone marrow transplantation can provide stromal cells able to synthesize intact collagen type I, replacing deficient patient cell function and ameliorating disease symptoms [5]. (issca.us)
  • Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)
  • A study by Hashem et al using induced pluripotent stem cell-derived cardiomyocytes indicated that LAMP2 protein deficiency in Danon disease results in autophagic flux impairment, which in turn causes excessive oxidative stress and, subsequently, cardiomyocyte apoptosis. (medscape.com)
  • From 1999 onwards my research has been focused primarily on osteoblast biology and approaches to tissue engineer and model bone biology, pathology and repair. (nottingham.ac.uk)
  • In contrast to the cost- and labor-intensive cultivation and propagation of cells such as MSCs or articular chondrocytes, the clinical use of "minimally processed" autologous BMA that can be prepared in the operation room as a single-step procedure appears straightforward. (hindawi.com)
  • 4. Felker GM, Shaw LK, O'Connor CM. A standardized definition of ischemic cardiomyopathy for use in clinical research. (tci-thaijo.org)
  • Several single gene disorders share clinical and radiologic characteristics with multiple sclerosis and have the potential to be overlooked in the differential diagnostic evaluation of both adult and paediatric patients with multiple sclerosis. (medscape.com)
  • The developmental story of cell-based cancer immunotherapy is not so different from MSCs in regenerative medicine. (biomedcentral.com)
  • In this study, molecular mechanisms of MSCs in regulating germ cell activity induced by testicular torsion-detorsion were investigated. (biomedcentral.com)
  • MSCs with 3 × 10 4 cells were locally injected into left testis 30 min before detorsion. (biomedcentral.com)
  • The tissue source of the MSCs was in most cases not decisive, and cells from various tissue sources were explored. (issca.us)
  • and signals from the injected cells were found early after administration of the MSCs at the highest frequencies in the lungs, followed by liver and spleen. (issca.us)
  • Since endometrial stromal cells are easy to isolate, and expand rapidly from patients without leading to major ethical and technical problems. (scirp.org)
  • Asmani, M.N., J. Ai, G. Amoabediny, A. Noroozi, M. Azami, S. Ebrahimi-Barough, M. Navaei-Nigjeh, A. Ai, M. Jafarabadi (2013) Three-dimensional culture of differentiated endometrial stromal cells to oligodendrocyte progenitor cells (OPCs) in fibrin hydrogel. (karger.com)
  • When these cells were combined with satellite cells from wt P10 mice and cultured, the two cell types were able to coalesce and regenerate skeletal muscle in vivo. (wikipedia.org)
  • In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)
  • Although the efficacy of adoptive transfer of tumor infiltrating lymphocytes (TILs) was examined over several decades, genetically engineered T cells expressing chimeric antigen receptors (CARs) rapidly replaced the application of TILs due to their high specificity, non-MHC-restricted recognition of tumor antigen, superior potency, and improved in vivo persistency [ 9 , 13 , 14 ]. (biomedcentral.com)
  • Here we generate a robust system for in vivo transgene delivery by combining the versatility of AAV-mediated transgene delivery with the specificity of cell-type-specific Cre-mediated recombination. (nature.com)
  • From these findings, researchers concluded that the donor cells are involved with the developing mesoderm and vasculature of host tissues. (wikipedia.org)
  • They are also characterized by their ability to migrate and integrate into damaged tissues and their capabilities of self-renewal, which allows them to maintain their stem cell properties over multiple passages. (wikipedia.org)
  • A few of the cells in the inner cell mass will develop into the foetus, whereas the rest will form the placenta and other supporting tissues needed for foetal development in the uterus. (biomedcentral.com)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • Cells Tissues Organs (2016) 202 (1-2): 85-101. (karger.com)
  • Animal cells need to be able to communicate with each other so that they can work together in tissues and organs. (elifesciences.org)
  • We hypothesized that AAV transgene expression could be targeted to specific tissues or cell types by selectively overexpressing AAVR. (nature.com)
  • All tissues originate from stem cells. (bvsalud.org)
  • A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)
  • Clare's lab recently identified a population of progenitor/stem cells in mice that, on transplantation, is sufficient to generate a fully functional thymus. (eurostemcell.org)
  • The differentiated ES cell cultures are heterogeneous and contain undifferentiated ES cells, which could result in teratoma formation after transplantation into the host. (biomedcentral.com)
  • In these studies, however, transplanted cells were followed, if at all, at the site of transplantation, and biodistribution was not an issue. (issca.us)
  • The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
  • In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
  • Specifically, human iPS cells could be utilized to generate patient-specific lineages for a variety of translational research. (biomedcentral.com)
  • Translational research that takes advantage of this new knowledge and applies it to diagnose and cure disease has proliferated in the constant search for better ways to treat our patients. (rmmj.org.il)
  • Rapid effects of steroid hormones on free intracellular calcium in T84 colonic epithelial cells. (sagepub.com)
  • These novel differentiated cells in fibrin gel might open new opportunities for the management of cell survival and neurotrophic potential in tissue engineering approaches for nerve repair. (scirp.org)
  • Hematopoietic (blood forming) stem cells have been investigated for several decades and is currently by far the best characterized stem cell system. (biomedcentral.com)
  • Until recently it was considered a fact that, for example, hematopoietic stem cells did not have the potential to differentiate into cells of other types than the cells constituting the blood system. (biomedcentral.com)
  • Diminished myelin levels lead to further cell death in which unmyelinated neurons die. (karger.com)
  • Superior specificity is achieved by combining Cre-mediated AAVR overexpression with whole-body knockout of endogenous Aavr , which is demonstrated in heart cardiomyocytes, liver hepatocytes and cholinergic neurons. (nature.com)
  • The activation of ligand-gated ionotropic P2X receptors and G protein-coupled metabotropic P2Y receptors has been reported to induce an increase of intracellular Ca2+ concentration ([Ca2+]i) in inner hair cells (IHCs), outer hair cells (OHCs), spiral ganglion neurons (SGNs), and supporting cells in the cochlea. (sagepub.com)
  • The basement membrane of myocytes is the boundary that separates the intracellular part of the cell from the extracellular environment. (microscopemaster.com)
  • The cells secrete extracellular vesicles (EV) that may have an endosomal origin, or from evaginations of the plasma membrane. (frontiersin.org)
  • On the other hand, the "exosome vesicle " is an extracellular particle released from the endosomal compartment of most eukaryotic cells. (frontiersin.org)
  • The exosome vesicles are a type of extracellular vesicles (EV), which are defined as lipid-bilayer spheroid structures, without replicating capacity, that are released from cells, including both prokaryotes and eukaryotes. (frontiersin.org)
  • Recent studies showed that extracellular ATP induced nitric oxide (NO) production in IHCs, OHCs, and SGNs, which affects the ATP-induced Ca2+ response via the NO-cGMP-PKG pathway in those cells by a feedback mechanism. (sagepub.com)
  • The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)
  • Quail donor cells colonized the vascular walls of chick wings, being especially prominent in skeletal muscle. (wikipedia.org)
  • In healthy young adults, the skeletal muscle mass comprises approximately 60% of total body mass. (frontiersin.org)
  • Mouse ES cells have been widely utilized as an in vitro model to study cardiogenesis, as cardiomyocytes were found to spontaneously differentiate from ES cells after withdrawal of LIF (leukemia inhibitory factor), which functions to maintain the pluripotency of undifferentiated mouse ES cells [ 2 - 4 ]. (biomedcentral.com)
  • A mesoangioblast is a type of progenitor cell that is associated with vasculature walls. (wikipedia.org)
  • A number of receptors are also found on the membrane of cardiomyocytes. (microscopemaster.com)
  • The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
  • Conclusions We conclude that ARAP2 affects Akt signaling in some cells by a mechanism independent of FAs or membrane traffic. (bio2009.org)
  • In the embryo of a mouse, for instance, precursor cells of the cardiac muscles have been shown to start developing about 6 days after fertilization. (microscopemaster.com)
  • Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. (cancerindex.org)
  • Acetaminophen 1999) has been found to regulate FA dynamics by control of Akt (Broussard et al. (bio2009.org)
  • This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
  • Both ES cells and iPS cells are pluripotent stem cells with capabilities of indefinite self-renewal and can be differentiated into almost all cell types of the body, which make them valuable for studying early developmental biology, for modeling and as therapy for human diseases. (biomedcentral.com)
  • Cell-based therapy has expanded its influence in cancer immunotherapy, regenerative medicine, and tissue engineering. (biomedcentral.com)
  • Cell surface engineering to provide new characteristics and functions to cells has drawn continuous interest from researchers in biomedical science as cell therapy has emerged as a prominent therapeutic strategy equivalent and complementary to the conventional therapeutic approaches. (biomedcentral.com)
  • There are different types of stem cells that best can be described in the context of normal human development [ 23 ]. (biomedcentral.com)
  • In human beings, as well as many other animals, cardiomyocytes are the first cells to terminally differentiate thus making the heart one of the first organs to form in a developing fetus. (microscopemaster.com)
  • Critical to the hypothesis is the experimental fact that human endometrial-derived stem cells (hEnSCs) as multipotent accessible source of cells are known as useful cell candidates in the field of nerve tissue engineering. (scirp.org)
  • These results show that for the first time, human endometrial stem cells can be differentiated into Schwann cells in 2D and 3D culture. (scirp.org)
  • Novel scientific discoveries achieved via on-going basic research has led to the expansion of human knowledge and a better understanding of the basic processes involved in life and disease. (rmmj.org.il)
  • Aung HH, Mehendale SR, Wang CZ, Xie JT, McEntee E. Cisplatin's tumoricidal effect on human breast carcinoma MCF-7 cells was not attenuated by American ginseng. (novapublishers.com)
  • This research often requires the tissue-specific expression of transgenes to better mirror human diseases, to visualize pathways being studied or to modulate the expression of a disease-related gene. (nature.com)
  • Therefore, the implantation of human cells reprogrammed in vitro represents an alternative strategy [ 16 - 19 ]. (thno.org)
  • Human stem cell factor contributed the activation of Akt/GSK3 axis when sperm suffered from testicular torsion-detorsion-induced germ cell injury. (biomedcentral.com)
  • Human sperm cell is a flagellate cell consisting of a disc-shaped head and a long tail released from the seminiferous tubule and mature in the epididymis. (biomedcentral.com)
  • The possible donor-host rejection of human ES cells is another concern 3 . (bvsalud.org)
  • To date, four types of human dental stem cells have been isolated and characterized: (i) dental pulp stem cells (DPSCs) 12 (ii) stem cells from exfoliated deciduous teeth (SHED) 21 (iii) stem cells from apical papilla (SCAP) 31 (iv) periodontal ligament stem cells (PDLSCs) 28 . (bvsalud.org)
  • I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
  • I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. (nottingham.ac.uk)
  • In recent years, the parallel progresses in cell biology, materials science, and advanced nano-manufacturing procedures have allowed us to envision the possibility to set up novel strategies (collectively dubbed "tissue engineering") to combine cells and biomaterials to fabricate myocardium-like structures in vitro to be engrafted into the heart to repair the damaged parts. (encyclopedia.pub)
  • Through them, the cells modify their microenvironment and the behavior of neighboring cells. (frontiersin.org)
  • The non-conductive component of the scaffold provides a biocompatible microenvironment to the cultured cells while the conductive component improves intercellular coupling as well as electrical signal propagation through the scar tissue when implanted at the infarcted site. (encyclopedia.pub)
  • Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
  • After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • To do so, cells release signaling molecules that can move around within a tissue and be detected by receptors on other cells. (elifesciences.org)
  • However, random variations (noise) that affect how many of these molecules are produced, how they move through the space between cells and how they bind to receptors makes the reality much more complex. (elifesciences.org)
  • The development of transgenic mouse models that express genes of interest in specific cell types has transformed our understanding of basic biology and disease. (nature.com)
  • We demonstrate that transgenic AAVR overexpression greatly increases the efficiency of transduction of many diverse cell types, including muscle stem cells, which are normally refractory to AAV transduction. (nature.com)
  • Ruengsakulrach P, Visudharom K, Chaothawee L, Belkin M. Safety and Efficacy of Intramyocardial Implantation of Peripheral Blood Stem Cell for Cardiomyopathy. (tci-thaijo.org)
  • The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)
  • The Netherlands Heart Foundation: 1997-1999. (umcutrecht.nl)
  • Also known as myocardiocytes, cardiomyocytes are cells that make up the heart muscle/cardiac muscle. (microscopemaster.com)
  • As the chief cell type of the heart, cardiac cells are primarily involved in the contractile function of the heart that enables the pumping of blood around the body. (microscopemaster.com)
  • Nano-thin and nano-porous membranes and heart like electric stimulus were used in the cardiac-mimetic cell-culture system. (thno.org)
  • In the heart, cardiomyocytes interact with neighboring cells through direct cell-cell interactions, various secreted cytokines, and electric signals (Figure 1 A) [ 24 ]. (thno.org)
  • As a clinician-scientist, I have a special interest in developing new stem cell based treatments for heart disease. (buffalo.edu)
  • The extent of reperfusion injury is directly proportional to preservation time in cold storage, and research has shown that with static storage methods, heart storage time will not exceed six hours. (gotomydoctor.com)
  • This theory describes that mesoangioblasts act as the precursors to certain cells in this region, as there is a possibility of a hematopoiesis-supporting element that contains mesodermal tissue progenitors. (wikipedia.org)
  • The final product is called angiogenic cell precursors (ACPs). (tci-thaijo.org)
  • Cell cycle gene expression networks discovered using systems biology: Significance in carcinogenesis. (genenetwork.org)
  • Morphogen gradients induce sharply defined domains of gene expression in a concentration-dependent manner, yet how cells interpret these signals in the face of spatial and temporal noise remains unclear. (elifesciences.org)
  • Multiple sclerosis is a common, chronic demyelinating neurological disease primarily affecting young adults, with a prevalence of ~0.1% in the Caucasian population (Miller and Leary, 2007). (medscape.com)
  • HCM is estimated to be present in 2 of every 1000 young adults, according to one large study. (medscape.com)
  • It serves as the mechanical linkage between the cardiac cells (cardiomyocytes) through the specialized intercalated disks. (microscopemaster.com)
  • In addition, it contributes to the excitation and contraction coupling through the transverse tubules (invaginations of the sarcolemma into the cytoplasm of cardiac cells). (microscopemaster.com)
  • This instigated the search for cells that could differentiate into cells of the mesodermal tissue. (wikipedia.org)
  • I work on various biochemical and biophysical approaches to investigate and control cell-cell, cell-scaffold interactions. (nottingham.ac.uk)
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
  • HN - 2008 BX - Granulosa Cells, Cumulus MH - Coronary Sinus UI - D054326 MN - A07.231.908.194.500 MS - A short vein that collects about two thirds of the venous blood from the MYOCARDIUM and drains into the RIGHT ATRIUM. (bvsalud.org)
  • Myocardial infarction (MI) is one of the clusters of numerous other cardiovascular diseases and occurs due to the blockage of the coronary artery delivering blood (ischemia) to the ventricle with consequent oxygen shortage to the contractile cells (cardiomyocytes) [ 1 ] . (encyclopedia.pub)
  • Combined coronary artery surgery and cell injection were performed in 33.8% of ICM cases. (tci-thaijo.org)