• 2002
  • The cardiac-specific nuclear δ B isoform of Ca 2+ /calmodulin-dependent protein kinase II induces hypertrophy and dilated cardiomyopathy associated with increased protein phosphatase 2A activity," Journal of Biological Chemistry , vol. 277, no. 2, pp. 1261-1267, 2002. (hindawi.com)
  • Ca 2+ /calmodulin-dependent protein kinase II is required for microcystin-induced apoptosis," Journal of Biological Chemistry , vol. 277, no. 4, pp. 2804-2811, 2002. (hindawi.com)
  • peptide
  • Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Calmodulin. (abcam.com)
  • Professor Matthias Rief and colleagues at the Technische Universitaet Muenchen had previously shown that they could fix a single calmodulin molecule between a surface and the cantilever tip of a specially built atomic-force microscope, expose it to calcium ions in solution, induce peptide binding and unbinding, and measure changes in the molecule's mechanical properties as it did its work. (innovations-report.com)
  • By applying mechanical force," Junker says, "we are able to dismantle the calmodulin-target peptide complex with surgical precision. (innovations-report.com)
  • Synthetic peptide corresponding to Human Calmodulin 1/2/3 aa 10-30 (C terminal) conjugated to keyhole limpet haemocyanin. (abcam.com)
  • Synthetic peptide corresponding to Human Calmodulin. (abcam.com)
  • antibody
  • Formalin-fixed paraffin-embedded human cancer tissue (breast carcinoma) with ab38590 anti-calmodulin, peroxidase-conjugated to the secondary antibody, followed by AEC staining. (abcam.com)
  • interact
  • We demonstrated that, in vitro, EP24.15 and calmodulin physically interact only in the presence of Ca2+, with an estimated Kd value of 0.52 mum. (nih.gov)
  • Target
  • Rief and Junker used mechanical force - actually pulling on complexes of calmodulin and the target peptides at rates of 1 nanometer per second or less - to slow down the processes to observable time scales and to clearly separate the individual unbinding steps. (innovations-report.com)
  • Original paper: "Single-molecule force spectroscopy distinguishes target binding modes of calmodulin," by Jan Philipp Junker and Matthias Rief, published in the online Early Edition of PNAS, Proceedings of the National Academy of Sciences for the week of August 10, 2009. (innovations-report.com)
  • cells
  • Together, these data suggest that the interaction between EP24.15 and calmodulin is regulated within cells and is important for the stimulated secretion of EP24.15 from HEK293 cells. (nih.gov)
  • Calmodulin (CaM) (an abbreviation for calcium-modulated protein) is a multifunctional intermediate calcium-binding messenger protein expressed in all eukaryotic cells. (wikipedia.org)