• Each sequence from this database was used to search public protein databases to assign putative function to as many database entries as possible. (usda.gov)
  • The amino acid sequence of Hda1p was used to search the Gen-Bank/expressed sequence tag databases to identify partial sequences from three putative class II human HDAC proteins. (psu.edu)
  • The localization of the HKE2 gene to the class II region, its cytoplasmic expression and putative protein-binding domain suggest that HKE2 may function in adaptive immunity and cancer. (genscript.com)
  • The GPCR97 clone was used to probe a human thalamus library, which resulted in the isolation of a full-length clone encoding a putative G protein-coupled receptor. (aspetjournals.org)
  • Of the four putative novel defence proteins (DFPs) annotated by PSI-BLAST three showed similarity to extracellular matrix proteins from vertebrates implicated in innate immunity, while the fourth was similar to, yet distinct from, the anti-microbial protein cecropin. (biomedcentral.com)
  • The present study has identified many potential immune-related genes in A. mylitta some of which are vertebrate homologues and others are hitherto unreported putative defence proteins. (biomedcentral.com)
  • The hOAT3 cDNA consisted of 2179 base pairs that encoded a 543-amino-acid residue protein with 12 putative transmembrane domains. (aspetjournals.org)
  • A high quality normalized cDNA library was constructed. (nih.gov)
  • The objectives of this study were to construct a normalized cDNA library for efficient EST analysis, to generate thousands of ESTs, and to analyze the ESTs for microsatellites and potential single nucleotide polymorphisms (SNPs). (nih.gov)
  • A total of 35 microsatellites were identified from the ESTs, with 18 having sufficient flanking sequences for primer design. (nih.gov)
  • A total of 5,542 ESTs were generated representing 4,688 unique sequences. (nih.gov)
  • From the two cDNA-libraries 24,000 ESTs were collected and a total of 2200 unigenes have been sequenced and annotated ( www.marinegenomics.org ). (vin.com)
  • Automatic comparisons of data from expressed sequence tags (ESTs) with genes (for example using the program Acembly, for which the results are available from the NCBI AceView server [ 2 ]) shows that all the human protein prenyltransferase genes have multiple alternative splice variants. (biomedcentral.com)
  • Expressed sequenced tags (ESTs) of 1 019 randomly selected clones were analyzed and then assembled 588 unigens. (korea.ac.kr)
  • The leaves were harvested at various times after cold stress for total RNA extraction, which was used to generate expressed sequence tags (ESTs). (geneticsmr.com)
  • After randomly choosing, cloning and sequencing, 227 ESTs with high quality were obtained and submitted to GenBank database. (geneticsmr.com)
  • Using BLASTX, 79.7% of the ESTs shared homology with known functional DNA sequences and 20.3% shared significant homology with unknown proteins. (geneticsmr.com)
  • Sequences of loblolly pine primer pairs from xylem ESTs and seedling ESTs suitable as anchor loci for comparative mapping. (ucdavis.edu)
  • We identified 719 unique ESTs from a total of 1412 sequences so generated. (biomedcentral.com)
  • Therefore, we constructed a cDNA library using algal cells grown at exponential growth phase and carried out expressed sequence tag (EST) analysis. (academicconcepts.net)
  • As described in Materials and Methods, we constructed a cDNA library from fat body tissues of E. coli - challenged A. mylitta larvae and randomly sequenced a large number of inserts from the library. (biomedcentral.com)
  • Trout TNFSF 13b RNA is expressed in immune tissues including the spleen, anterior kidney, and peripheral blood leukocytes but not in the liver. (usda.gov)
  • These class II HDAC proteins have differential mRNA expression in human tissues and possess in vitro HDAC activity that is inhibited by trichostatin A. Coimmunoprecipitation experiments indicate that these HDAC proteins are not components of the previously identified HDAC1 and HDAC2 NRD and mSin3A complexes. (psu.edu)
  • Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags. (genscript.com)
  • The prepared antibody can be useful for the study of expression and distribution of IL-33 in various tissues at protein level and for the elucidation of its biofunctions and regulation mechanism in inflammatory diseases. (hindawi.com)
  • RESULTS: Here, we report the characterization of a cDNA, 1RT126I22, which contains a TNF homology domain. (usda.gov)
  • Bacterial artificial chromosome clones have been isolated from a trout library to further facilitate genomic characterization. (usda.gov)
  • 1] "Complete sequencing and characterization of 21,243 full-length human cDNAs. (tcdb.org)
  • 4] "In silico identification and expression of SLC30 family genes: an expressed sequence tag data mining strategy for the characterization of zinc transporters' tissue expression. (tcdb.org)
  • We used the in vitro / in vivo dual expression system plasmid, pIVEX, to express Danio rerio pol β ( Danio pol β) for biochemical characterization. (biomedcentral.com)
  • The 2200 unigenes from the EST collection and the 62 immune-function targeted genes, together with other genes randomly selected without sequencing from the cDNA libraries, have been amplified and used to construct a cDNA microarray representing 3700 dolphin genes in duplicate for a total of 7400. (vin.com)
  • The cDNA dolphin array will be used to analyze PBL RNA from captive and wild dolphins of known health status with the aim to validate and optimize the cDNA microarray as a sensitive and informative tool for dolphin health assessment. (vin.com)
  • cDNA microarray data (7). (lu.se)
  • Sequence homology is an indication of the genetic relatedness of different organisms and gene function. (lookformedical.com)
  • Its cDNA sequence contained an open reading frame encoding a 431-amino acid peptide that showed a significant homology with members of the P2X family. (aacrjournals.org)
  • As part of a directed effort to discover novel G protein-coupled receptors through homology searching of expressed sequence tag databases, we identified a partial clone (GPCR97) that had significant homology to biogenic amine receptors. (aspetjournals.org)
  • The genomic organization of the human genes that encode protein prenyltransferases is shown in Figure 1 . (biomedcentral.com)
  • The IL-33 cDNA sequences encode 270 and 266 amino acid polypeptides for human and mouse, respectively, corresponding to full-length proteins with calculated masses of 30 and 29.9 kDa [ 2 ]. (hindawi.com)
  • Two cDNA libraries from known health status dolphin PBL have been generated including an IL-2 and an LPS-stimulated cDNA library, respectively biased towards T and B-cell gene expression. (vin.com)
  • ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
  • Almost 2,000 expressed sequence tag (EST) cDNA clones were sequenced to analyze gene expression in control and Cd-treated leaf trichomes. (uni-bayreuth.de)
  • The expression of osmotin and thaumatin-like proteins was induced by Cd treatment in both leaves and trichomes. (uni-bayreuth.de)
  • The expression of this gene at the protein level was further confirmed in the spleen, thymus, and bursa of Fabricius of NDV-infected chickens using Western blot analysis. (geneticsmr.com)
  • Although we detected no genetic alteration in the coding sequences, one of four rhabdomyosarcoma cell lines examined had completely lost expression of this gene. (aacrjournals.org)
  • Strategies and Components Cloning of MSL constructs For heterologous manifestation and purification, MSL constructs had been cloned with C-terminal FLAG tags in to the pFastBac1 vector, that was found in the Bac-to-Bac expression system (Invitrogen) to create recombinant baculoviruses. (monossabios.com)
  • The CXC domain was additionally cloned into the pGEX-2KG expression vector (Amersham) for expression as a GST fusion protein in BL21-CodonPlus (DE3)-RIL cells (Stratagene). (monossabios.com)
  • Heterologous expression of MSL proteins MSL proteins were expressed in Sf21 cells using recombinant baculoviruses. (monossabios.com)
  • The coding region of IL-33 mature protein was cloned into the prokaryotic expression vector pET-44. (hindawi.com)
  • In this study, the cloning and expression of mouse IL-33 gene in Escherichia coli (E. coli ), purification of recombinant protein, and generation of polyclonal antibody against IL-33 are described. (hindawi.com)
  • In the vertebrate CNS, HB9/MNR2 transcription factors are specifically expressed in all somatic motoneurons and are necessary to distinguish motoneurons from interneurons, in part by repressing interneuron-specific gene expression. (jneurosci.org)
  • Mouse HB9 maintains expression in somatic motoneurons, whereas chick HB9 is expressed only in postmitotic somatic motoneurons. (jneurosci.org)
  • The transient expression in tobacco mesophyll cells using Green Fluorescent Protein tagging determined the subcellular localization of CtCHI in cell membrane and nucleus. (springeropen.com)
  • This clone was engineered to express the complete ORF with an expression tag. (origene.com)
  • Furthermore, the encoded protein contained two transmembrane regions without signal peptides, indicating that this protein is located in the mitochondrial membrane. (geneticsmr.com)
  • IL1RAPL2 is a type I transmembrane protein that contains three Ig-like domains in its extracellular region and one intracellular TIR domain. (rndsystems.com)
  • These motifs contain 2 hydrophobic alpha-helical segments connected by an extensive hydrophilic sequence, resulting in 6 transmembrane portions of the protein. (medscape.com)
  • Although a recent study ( 22 ) reported enhanced avidity of H5N6 viruses for human-type receptors, recombinant clade 2.3.4.4 highly pathogenic influenza A virus H5 proteins from virus isolates in North America show a strict avian receptor-binding preference ( 23 ). (cdc.gov)
  • Having a biochemical strategy, we now have sought out a DNA binding site within recombinant MSL2 and MSL1 protein by calculating their affinities to described DNA sequences, including Offers. (monossabios.com)
  • Purification of Goat polyclonal to IgG (H+L)(FITC) recombinant MSL proteins Sf21 cell pellets had been quickly thawed and resuspended in ice-cold Removal Buffer EB (50 mM Hepes/KOH pH 7.6, 5% glycerol, GSK690693 cost 0.05% NP-40, 0.5 mM EDTA, 1 mM MgCl2, protease inhibitors Aprotinin 1 g/ml, Leupeptin 1 Pepstatin and g/ml 0.7 g/ml) containing 300 mM KCl (EB300). (monossabios.com)
  • The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. (betalifesci.com)
  • The recombinant protein, IL-33 containing a hexahistidine tag in the C-terminal, was expressed in Escherichia coli . (hindawi.com)
  • The rabbits were immunized with the purified recombinant protein. (hindawi.com)
  • The high titer (1:32000) polyclonal antibodies with high specificity were obtained by immunizing rabbits with the purified recombinant protein. (hindawi.com)
  • The precursor protein (pre-IL-33) was cleaved by caspase-1 into a mature 18-kDa protein (IL-33) in in vitro experiments using a recombinant protein [ 2 ]. (hindawi.com)
  • Recombinant Mpn HPRT was expressed as an N-terminal fusion protein with a 6 × histidine tag and a tobacco etch virus (TEV) cleavage site at the N-terminus, and was purified to >98% purity by metal affinity chromatography, see more as assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis (data not shown). (bcl-2protein.com)
  • Three introns were identified in the rainbow trout, Atlantic salmon and zebrafish genomic sequences, and the intron locations correspond closely to those in the mammalian TNFSF 13b genes. (usda.gov)
  • endonuclease (CuquEndo) produced in mammalian cells showed no sequence specificity for DNA substrate except that it only cleaves double-stranded DNA. (silverchair.com)
  • The cDNA of Danio rerio pol β encodes a protein of 337 amino acids, which is 2-3 amino acids longer than other pol β proteins, and contains a P63D amino acid substitution, unlike mammalian pol βs. (biomedcentral.com)
  • The transporter was identified by probing a mammalian-expressed sequence tag database with 2 fungal mitochondrial ornithine carrier protein sequences. (medscape.com)
  • Of the 233 randomly selected clones, 25 were identified as differentially expressed genes between 5MT resistant and 5MT sensitive wild type plants. (korea.ac.kr)
  • We identified zebrafish and Fugu TNFSF 13b homologues by genomic DNA database searches and determined that the predicted proteins share 68% and 76% sequence identity with trout TNFSF 13b. (usda.gov)
  • Studies conducted on PRPF31 mRNA levels in lymphoblast cell lines isolated from family members with either deletion or splice site mutations indicate that penetrance requires the co-inheritance of a low expressing wild-type (WT) allele alongside the mutant allele [ 13 , 14 ], whereas the presence of a high expressing WT allele is protective. (molvis.org)
  • An expressed sequence tag (EST) library was constructed from mRNA obtained from fat bodies of A. mylitta larvae that had been challenged by infection with Escherichia coli cells. (biomedcentral.com)
  • Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. (aspetjournals.org)
  • The full-length complementary DNA (cDNA) of heat shock protein 90 was cloned from Phascolosoma esculenta (PeHSP90) using expressed sequence tag and rapid amplification of cDNA end approaches. (geneticsmr.com)
  • Ornithine incorporation was restored in fibroblasts derived from patients with hyperornithinemia-hyperammonemia-homocitrullinuria syndrome by transforming the fibroblasts with transporter complementary DNA (cDNA). (medscape.com)
  • Isolation of markers for chondro-osteogenic differentiation using cDNA library subtraction. (wikipedia.org)
  • Approximately 5,400 clones from each library were sequenced from both the 5' and 3' directions. (usda.gov)
  • A normalized and subtracted C. virginica cDNA library was constructed from pooled RNA isolated from hemocytes, mantle, gill, gonad and digestive tract, muscle, and a whole juvenile oyster. (nih.gov)
  • A total of 6,528 clones were sequenced from this library generating 5,542 high-quality EST sequences. (nih.gov)
  • To evaluate the 5MT resistance mechanism, a cDNA library was constructed by using the leaves and roots of the 5MT resistant mutant plants. (korea.ac.kr)
  • A cDNA library of Chinese wild Vitis amurensis , which is the most cold-resistant species in the genus Vitis , was constructed using young leaves of seedlings of the clone Heilongjiang potted and subjected to cold stress. (geneticsmr.com)
  • Some functional genes identified from the cDNA library have potential for plant defense. (geneticsmr.com)
  • In the present study, 795 cotton tubulin expressed sequence tags were analysed and 19 beta-tubulin genes (TUB) cloned from a cotton cDNA library. (singerinstruments.com)
  • In order to investigate the molecular mechanism of rehydration after serious drought in broomcorn millet ( Panicum miliaceum L.), a forward subtracted cDNA library was constructed between normal watered leaves and rehydrated leaves after serious drought conditions, using the suppressive subtraction hybridization (SSH) technique. (cambridge.org)
  • A cDNA encoding a multispecific organic anion transporter 3 (hOAT3) was isolated from a human kidney cDNA library. (aspetjournals.org)
  • Mitochondrial and chloroplast proteins are therefore synthesized in the cytosol as preproteins with an NH 2 -terminal targeting sequence, which is both necessary and sufficient for directing the preproteins to the correct organelle. (silverchair.com)
  • As with other mitochondrial carrier family proteins, the ornithine carrier is composed of 300 amino acids that constitute 3 repeated motifs of approximately 100 amino acids each. (medscape.com)
  • Mpn HPRT (MPN672) consists of 175 amino acids and shares 29% sequence identity to human HPRT. (bcl-2protein.com)
  • This protein also revealed a similarity to RP-2 , a gene activated in thymocytes undergoing programmed cell death. (aacrjournals.org)
  • Missense mutations in PRPF31 have previously been shown to cause reduced protein solubility, suggesting insufficiency of functional protein as the disease mechanism. (molvis.org)
  • You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. (betalifesci.com)
  • The corresponding full-length cDNAs were cloned and defined as HDAC4, HDAC5, and HDAC6. (psu.edu)
  • The genes encoding CaaX protein prenyltransferases are considerably longer than those encoding non-CaaX subunits, as a result of longer introns. (biomedcentral.com)
  • Two normalized cDNA libraries were constructed from RNA isolated from embryos and 2nd instar larvae from the Panama 95 strain. (usda.gov)
  • cDNA libraries were constructed from control and cadmium (Cd)-treated leaf trichomes. (uni-bayreuth.de)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • Following recognition of TLR ligands, TLRs elicit innate immunity by activating multiple intracellular signaling cascades including variable adaptor proteins and transcriptional factors. (frontiersin.org)
  • Additionally, double-stranded cDNA was prepared from random-primed polyA RNA purified from embryos, 2nd instar larvae, adult males and adult females. (usda.gov)
  • We also identified several unknown proteins, some of which could have probable immune-related functions based on the results of the ProDom analysis. (biomedcentral.com)
  • The Alu sequence family (named for the restriction endonuclease cleavage enzyme Alu I) is the most highly repeated interspersed repeat element in humans (over a million copies). (lookformedical.com)
  • A total of 1,960 nonredundant sequences (NRSs) were generated for N. oculata clone CS-179. (academicconcepts.net)
  • Cysteine-rich PR proteins, such as non-specific lipid transfer proteins (nsLTPs) and metallocarboxypeptidase inhibitors, are candidates for the sequestration of metals. (uni-bayreuth.de)
  • A total of 62 dolphin genes of known stress or immune function have been cloned by targeted PCR (including immunoglobulins, T-cell receptors, cytokines, Toll-like receptors, stress proteins and toxin response proteins). (vin.com)
  • The cDNA for bovine IF2 mt was expressed in Escherichia coli under the control of the T7 polymerase promoter in a vector that provides a His 6 -tag at the C-terminus of the expressed protein. (elsevierpure.com)
  • This factor was expressed in E. coli and purified by chromatography on Ni-NTA resins. (elsevierpure.com)
  • The portion of the sequence encoding the region of human IF3 mt predicted to be present in the mitochondrially imported form of this factor was cloned and expressed in E. coli using a vector that provides a C-terminal His 6 -tag. (elsevierpure.com)
  • To obtain a fusion protein and prepare rabbit anti-serum, the predicted ORF of this gene was expressed in Escherichia coli . (geneticsmr.com)
  • Mpn HPRT cDNA was cloned and expressed in E. coli. (bcl-2protein.com)
  • Danio pol β encoded by the in vitro / in vivo -compatible pIVEX plasmid was expressed in E. coli BL21(DE3), BL21(DE3)pLysS, and KRX, and in vitro as a C-terminal His-tagged protein. (biomedcentral.com)
  • The protein kinase is found in the cytosol of pea mesophyll cells or in wheat germ extract. (silverchair.com)
  • These can be categorized into two classes: the first class represented by yeast Rpd3-like proteins and the second by yeast Hda1-like proteins. (psu.edu)
  • We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
  • The α subunits of protein prenyltransferases consist of tetratricopeptide repeats and are part of the tetratricopeptide repeat superfamily [ 5 ], which also includes functionally diverse proteins involved in transcription, co-chaperoning, protein transport, cell-cycle control and phosphorylation. (biomedcentral.com)
  • High-resolution mapping of the protein interaction network for the human transcription machinery and affinity purification of RNA polymerase II-associated complexes. (genscript.com)
  • Reverse transcription-PCR (RT-PCR) analysis demonstrated that antipathogenic T-phylloplanin-like proteins, glutathione peroxidase and several classes of pathogenesis-related (PR) proteins were expressed specifically or dominantly in trichomes. (uni-bayreuth.de)
  • The region of DNA which borders the 3' end of a transcription unit and where a variety of regulatory sequences are located. (lookformedical.com)
  • Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. (lookformedical.com)
  • Transcription profiling revealed that nine GhTUBs were highly expressed in elongating fibre cells as compared with fuzzless-lintless mutant ovules. (singerinstruments.com)
  • Together, vertebrate HB9/MNR2 transcription factors are expressed in all somatic motoneurons and excluded from interneurons. (jneurosci.org)
  • Altered receptor-binding specificity of virus clade 2.3.4.4 H5 proteins might have contributed to emergence and spread of H5Nx viruses. (cdc.gov)
  • The availability of the human H 3 receptor cDNA should greatly aid in the development of chemical and biological reagents, allowing a greater appreciation of the role of histamine in brain function. (aspetjournals.org)
  • Interleukin 1 receptor accessory protein-like 2 (IL1RAPL2), also known as TIGIRR1, is a member of the IL-1 RI-like family of the IL-1R/Toll-like receptor superfamily. (rndsystems.com)
  • Gene structures and chromosomal locations of human protein prenyltransferase subunit genes. (biomedcentral.com)
  • The precursor forms of prominent chloroplast proteins like the small subunit of stromal Rubisco (SSU), the thylakoid a/b binding protein (LHCP), or the thylakoid lumen localized subunits of the oxygen evolving complex of 23 kD and 33 kD (OE23, OE33) contain a phosphorylation site within the chloroplast targeting signal ( Waegemann and Soll 1996 ). (silverchair.com)
  • The need for the DNA binding function from the CXC site as well as for X chromosome focusing on is verified by reporter gene assays and localization research concerning GFP fusion proteins in cells. (monossabios.com)
  • The hOAT3 protein was shown to be localized in the basolateral membrane of renal proximal tubules and the hOAT3 gene was determined to be located on the human chromosome 11q12-q13.3 by fluorescent in situ hybridization analysis. (aspetjournals.org)
  • The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)
  • The result of Sanger sequencing and phylogenetic analysis revealed that CtCHI is highly homologous to other plants, including typical polyadenylation signals AATAA and Poly A tail. (springeropen.com)
  • In many cases, the "melting profile" of the amplification product predicted by the EST sequence was observed using MacMelt software (BioRad). (ucdavis.edu)
  • We used an expressed sequence tag and 454 pyrosequencing approach to initiate a study of the genome of the New World Screwworm, Cochliomyia hominivorax (Coquerel). (usda.gov)
  • There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. (lookformedical.com)
  • RNA Isolation and cDNA Microarrays. (lu.se)
  • In vertebrates, chick MNR2 and mouse HB9 are expressed in presumptive somatic motoneuron progenitors. (jneurosci.org)
  • Drosophila genes expressed specifically in all motoneurons have not been described, although a growing number of genes are known to be expressed in subsets of motoneurons. (jneurosci.org)
  • However,some nucleases showing little sequence specificity do recognize certain structural features of their respective DNA substrates other than ss/ds DNA. (silverchair.com)
  • Non-natural oligonucleotides are valuable probes of biological systems, as they can convey synthetic control of endogenous nucleic acids with high sequence specificity. (cdc.gov)
  • The following PFDN6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
  • A total of 389 unigenes had significant homologies with known protein sequences at the NCBI database and the remaining 199 unigenes were designated unidentified genes. (korea.ac.kr)
  • We have initiated study of the genes of the New World Screwworm, Cochliomyia hominivorax, by synthesizing and sequencing DNA libraries of expressed genes from embryos, 2nd instar larvae, adult males and adult females. (usda.gov)
  • DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom. (lookformedical.com)
  • therefore, bacterial overexpression was used to produce the protein for kinetic analyses. (biomedcentral.com)
  • The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. (lookformedical.com)
  • To define the localization of WDFY2 in the endocytic pathway, we transiently transfected hTERT-RPE1 cells with green flourescent protein (GFP)-WDFY2 and performed structured illumination microscopy (SIM) together with APPL1 and EEA1 visualized with antibodies (Fig. 1a ). (nature.com)
  • ER stress-inducible protein MANF selectively expresses in human spleen. (genscript.com)
  • The HsCXC domain from GSK690693 cost the human protein KIAA1585 was isolated via PCR from cDNA of HeLa cells and cloned into the vectors described above. (monossabios.com)
  • The soluble proteins small fraction was incubated with equilibrated FLAG beads (Anti-FLAG M2 Agarose, Sigma) for 2.5 h at 4C on the rotating wheel. (monossabios.com)
  • 2] "The full-ORF clone resource of the German cDNA consortium. (tcdb.org)
  • EU COST Next generation sequencing data analysis network and training consortium. (mpg.de)
  • Northern blot analysis showed that it was expressed predominantly in skeletal muscle. (aacrjournals.org)
  • Furthermore, it can be cross-linked in intact chloroplasts to a high molecular weight complex containing both Toc and Tic subunits and a precursor protein. (silverchair.com)
  • A phosphorylated precursor protein can still bind to the chloroplast import machinery, but must become dephosphorylated before translocating through the membranes. (silverchair.com)