• Isolation of clonable DNA (genomic libraries, cDNA synthesis) cloning vectors (plasmids, bacteriophages, cosmids) plasmid incompatibility and control of copy number. (up.ac.za)
  • This robust enzyme mix with proofreading activity will polymerize probes 40 bp to 5 kb long using 10 pg plasmid DNA and 10 ng genomic DNA as template. (thomassci.com)
  • The clones bear about 80% sequence homology with the respective rat cDNAs in the sequenced regions and hybridize with the rat clones in 5 X SSC at 55°C. The ovine LHβ clone has an insert of about 650 bp and selects an RNA of about 750 bases in a northern blot. (ias.ac.in)
  • An Infectious cDNA Clone of SARS-CoV-2. (cdc.gov)
  • Described in this protocol is the lipofectamine transfection of plated cells with cDNA and/or siRNA. (ohsu.edu)
  • A handy calculation spreadsheet is included to calculate siRNA and cDNA/plasmid concentrations and volumes. (ohsu.edu)
  • CytoSections are ready-to-use FFPE sections of cell pellets expressing targeted protein derived from gene-specific cDNA clones. (origene.com)
  • Welcome to Reportergene IMAGE clones, Plasmids & Lentivectors! (reportergene.com)
  • We have isolated cDNA clones for ovine α -subunit and LHβ. (ias.ac.in)
  • In biotechnology, the principle of base pair complementarity allows the generation of DNA hybrids between RNA and DNA, and opens the door to modern tools such as cDNA libraries. (wikipedia.org)
  • The BCCM/GeneCorner Plasmid Collection warrants the long-term storage and distribution of plasmids, microbial host strains and DNA libraries of fundamental, biotechnological, educational or general scientific importance. (go.jp)
  • We have primers to sequence verify gene inserts for most of the plasmids avialable in the TOPSAN repository. (topsan.org)
  • PlasClass improves plasmid sequence classification. (cdc.gov)
  • Transfection of NIH3T3 cells with the pcDNA3.1 expression vector containing the TIF3 cDNA in the sense (5'-->3') orientation resulted in overexpression of the encoded 36 kDa protein. (cdc.gov)
  • Addgene: Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties. (addgene.org)
  • Through cell survival-based cDNA expression screens in neural progenitor cells, we identify a genetic variant of AGS Atp5g1 that confers cell resilience to metabolic stress. (elifesciences.org)
  • Addgene is a nonprofit plasmid repository. (addgene.org)
  • A cDNA library of ovine pituitary DNA in plasmid pBR322 has been constructed by conventional methods with certain modifications. (ias.ac.in)
  • Cotransfection of the cells with a mixture of plasmid DNA consisting of TIF3 cDNA in the sense and in the antisense orientation resulted in significant inhibition of translation of the TIF3 protein. (cdc.gov)
  • The focus is on the collection of recombinant plasmids that can replicate in a microbial host strain. (go.jp)
  • gplas: a comprehensive tool for plasmid analysis using short-read graphs. (cdc.gov)
  • Learn about the latest plasmid technologies and research tools. (addgene.org)
  • A cDNA microarray was utilized to identify potential downstream targets of Hh signalling. (jcancer.org)
  • it has two internal Pst I sites and thus shows restriction-based differences from rat α -subunit cDNA, which does not have any Pst I site. (ias.ac.in)
  • Sets of BALB/c mice had been nasally immunized every week for three consecutive weeks with OVA and a combined mix of plasmid encoding the Flt3 ligand cDNA (pFL) and CpG oligodeoxynucleotides (CpG ODN) (solid design), pFL only (striped design), or CpG ODN only (open design) as nose adjuvants. (institutopsicofarmacologia.com)
  • Stability and expression of a plasmid-containing killer toxin cDNA in batch and chemostat cultures ofsaccharomyces cerevisiae. (ncsu.edu)
  • The effects of dilution rate, D(h(-1)) on plasmid stability and toxin expression were studied in transformed AH22 (AH22/T3) and Montrachet 522 (522/T1) wine yeast grown in glucose-limited chemostat cultures. (ncsu.edu)
  • Expression of the cDNA in E. coli yielded the active enzyme and showed immunoreactivity with antibodies against the rat liver PTPS. (nih.gov)
  • Expression of CoFad2 cDNA in yeast (Saccharomyces cerevisiae) indicated it encodes a Delta12 desaturase that introduces a double bond at the 12 position of 16:1(9Z) and 18:1(9Z). (researchgate.net)
  • And the cDNA cloning team at the Center for Eukaryotic Structural Genomics (CESG) at the University of Wisconsin, Madison, used Gateway to produce between 4,000 and 5,000 swappable expression constructs during phase 1 of its work for the National Institutes of Health-funded Protein Structure Initiative. (the-scientist.com)
  • in situ hybridisation to tissue sections using cDNA probe generated from a whole insert of pMY51 reveals that the gene has expression patterns in the cell bodies of the ellipsoid body and the Malpighian tubules consistent with the X-Gal staining. (gla.ac.uk)
  • 12. Antitumor efficacy of a human interleukin-12 expression plasmid demonstrated in a human peripheral blood leukocyte/human lung tumor xenograft SCID mouse model. (nih.gov)
  • This approach ( Figure 1 ) is based on the availability of a cognate gene plasmid and closely approximates the natural gene expression in yeast. (ejbiotechnology.info)
  • This vector contains 35S CaMV promoter for a constitutive cDNA expression and gateway recombination cassette to introduce your cDNA. (nibb.ac.jp)
  • Transfection of NIH3T3 cells with the pcDNA3.1 expression vector containing the TIF3 cDNA in the sense (5'-->3') orientation resulted in overexpression of the encoded 36 kDa protein. (cdc.gov)
  • simian virus 40 expression vectors contain- ing the chicken M1 PK cDNA (RL142PK10X, RL18PK8X, and RL18PK12X, which differ by the reading frame at a * Corresponding author. (nih.gov)
  • Overexpression of SsDREB cDNA in transgenic tobacco plants exhibited an improved salt and drought stress tolerance in comparison to the nontransformed controls. (hindawi.com)
  • If you are used to gateway system, it's easy to construct a transient overexpression plasmid. (nibb.ac.jp)
  • Using the ReViTA plasmid, transcription induction of 55% was observed when AlgR protein was added and a 27% transcription reduction with the repressor LexA, compared with the samples without transcription factors. (ibecbarcelona.eu)
  • Cotransfection of the cells with a mixture of plasmid DNA consisting of TIF3 cDNA in the sense and in the antisense orientation resulted in significant inhibition of translation of the TIF3 protein. (cdc.gov)
  • Plasmids encode regulators of G protein signalling (RGS). (nih.gov)
  • New Brunswick Scientific I2500 shaking incubators are used for growing bacteria during plasmid and protein production at 32C and 37C. (nih.gov)
  • Chemically competent E. coli cells suitable for subcloning efficiency transformations, such as plasmid transformation or routine subcloning (e.g,. inserting a 1 kb fragment into a 2 kb vector). (neb.com)
  • Oftentimes, those sequences are present on two separate plasmids, as when moving an insert from a starting ("entry") construct to a "destination" vector. (the-scientist.com)
  • 15. Targeted killing of carcinoembryonic antigen (CEA)-producing cholangiocarcinoma cells by polyamidoamine dendrimer-mediated transfer of an Epstein-Barr virus (EBV)-based plasmid vector carrying the CEA promoter. (nih.gov)
  • A cognate gene plasmid was constructed consisting of the pRS416 shuttle-vector in which the yeast open reading frame (ORF) and its regulatory elements were cloned. (ejbiotechnology.info)
  • The functional impact of each isoform was evaluated by transfecting cDNA plasmids specific to each isoform into NSCLC cell lines and comparing behavior to empty vector controls in scratch closure, cell proliferation, soft-agar colony formation, and Matrigel invasion assays. (nih.gov)
  • A chimeric plasmid (pYT760-ADH1) containing the yeast killer toxin-immunity cDNA was transformed into a leucine-histidine mutant (AH22) and into four industrial toxin-sensitive yeasts. (ncsu.edu)
  • So far, cognate plasmids are available for about 10% of the 6000 yeast genes, and may be obtained from the EUROSCARF collection index . (ejbiotechnology.info)
  • It is capable of sequencing DNA from various sources (plasmids, viral vectors, cells). (nih.gov)
  • Two homologous cDNAs, CoFad2 and CoFac2, were isolated from a Calendula officinalis developing seed by a polymerase chain reaction-based cloning strategy. (researchgate.net)
  • It is used in our standard quality assurance (QA) assays during plasmid and virus production as well as conventional and CRISPR-related genotyping assays. (nih.gov)
  • Quality Control Functional Assay cDNA synthesis with specific primers, followed by quantitative PCR. (milleranalytical.com)
  • A plasmid encodes human C-C chemokine receptor type 2 (CCR2). (nih.gov)
  • But Invitrogen also has systems for cloning PCR fragments directly into Gateway plasmids using Clonase (that is, without restriction enzymes and ligase). (the-scientist.com)
  • This goal was accomplished using a full length AhR cDNA plasmid linked to the immunoglobulin enhancer/promoter region. (nih.gov)
  • In this study, a novel cDNA encoding DREB transcription factor, designated SsDREB , was isolated from succulent halophyte Suaeda salsa . (hindawi.com)
  • After the promoters were incubated with the transcription factors, the plasmid was transcribed into RNA and reverse transcribed to cDNA. (ibecbarcelona.eu)
  • This approach is based on the use of the widely available cognate gene plasmids ( e.g. pRS416) of the European Functional Analysis Network (EUROFAN). (ejbiotechnology.info)
  • Cut the human DNA and bacterial plasmids with the restriction enzyme. (dummies.com)
  • r\nIf you cut a plasmid DNA and human DNA with the same restriction enzyme, all the DNA fragments will have the same sticky ends. (dummies.com)
  • Recombinase-based methods allow scientists to move pieces of DNA from plasmid to plasmid without restriction enzymes, simplifying and expediting the cloning process. (the-scientist.com)
  • Scientists get around this problem by creating intron-free eukaryotic genes in the form of complementary DNA (cDNA). (dummies.com)
  • These were used to screen samples of gDNA (prepared from PBMC) or cDNA (prepared from total RNA extracted using the Paxgene system from Preanalytix, UK) from CFS and normal blood donors. (medscape.com)
  • Steve Elledge, the Gregor Mendel Professor of Genetics at Harvard University, developed the system (which he called the univector plasmid-fusion system, or UPS) when he was at Baylor College of Medicine in 1998. (the-scientist.com)
  • This plasmid has an advantage to pTFH22.4 because of its smaller size. (nibb.ac.jp)
  • It is also capable of sequencing cDNA and RNA samples. (nih.gov)
  • It is T1 phage resistant and endA deficient for high-quality plasmid preparations. (neb.com)
  • r\nThe two types of DNA have the same sticky ends, so some pieces of plasmid DNA and human DNA will stick together. (dummies.com)
  • We overexpressed the gene from plasmids containing full-length and truncated versions of human PNPLA2 cDNA fused to His6x/Xpress peptide tags at their N-termini. (nih.gov)
  • It suggested that plasmid stability was dependent on the presence of the 2mu plasmid which is naturally present in some yeasts. (ncsu.edu)