• polymerase chain re
  • Detectable by polymerase chain reaction, STSs are useful for localizing and orienting the mapping and sequence data reported from many different laboratories and serve as landmarks on the developing physical map of the human genome. (biology-online.org)
  • With amplification of DNA sequences via polymerase chain reaction (PCR) now commonplace, one will typically conduct reverse transcription as an initial step, followed by PCR to obtain an exact sequence of cDNA for intra-cellular expression. (wikipedia.org)
  • phenotype
  • Genotype is the term applied to specific changes in DNA sequence found in a mutant, while phenotype refers to all biological consequences from the presence of the mutation in question. (nybg.org)
  • analysis
  • Furthermore, 97 genes were found to be expressed at a higher level in the flower than the leaf by the Significance Analysis of Microarray (SAM) method with a 1.0% false discovery rate (FDR). (deepdyve.com)
  • Genetics
  • On June 13, 2013, the United States Supreme Court ruled in the case of Association for Molecular Pathology v. Myriad Genetics that while naturally occurring human genes cannot be patented, cDNA is patent eligible because it does not occur naturally. (wikipedia.org)
  • bases
  • The CpG sites or CG sites are regions of DNA where a cytosine nucleotide is followed by a guanine nucleotide in the linear sequence of bases along its 5' → 3' direction . (wikipedia.org)
  • Daughter cells that inherit these wrong bases carry mutations from which the original DNA sequence is unrecoverable (except in the rare case of a back mutation, for example, through gene conversion). (wikipedia.org)
  • CpG islands are generally 200 to 2000 base pairs long, have a C:G base pair content >50%, and have regions of DNA where a cytosine nucleotide is followed by a guanine nucleotide and this occurs frequently in the linear sequence of bases along its 5' → 3' direction. (wikipedia.org)
  • single-stranded
  • CpG should not be confused with GpC , the latter meaning that a guanine is followed by a cytosine in the 5' → 3' direction of a single-stranded sequence. (wikipedia.org)
  • promoter
  • When FEN1 is over-expressed (this occurs when its promoter is hypomethylated) the highly inaccurate MMEJ pathway may be favored, causing a higher rate of mutation and increased risk of cancer. (wikipedia.org)
  • When FEN1, Ligase III, MRE11, NBS1, PARP1 or XRCC1 are over-expressed (this occurs with FEN1 when its promoter is hypomethylated) the highly inaccurate MMEJ pathway may be favored, causing a higher rate of mutation and increased risk of cancer. (wikipedia.org)
  • However, in cancer cells, CpG islands preceding tumor suppressor gene promoters are often hypermethylated, while CpG methylation of oncogene promoter regions and parasitic repeat sequences is often decreased. (wikipedia.org)
  • species
  • Like RFLP loci, STS loci can be analyzed as co-dominance genetic markers and can in theory, be studied in member of the species or closely related species, provided that the DNA sequence is conserved at the PCR primer sites. (biology-online.org)
  • Project
  • M. Maeda and her collaborators carried out cDNA project of the slime mold D. discoideum. (nii.ac.jp)
  • made
  • The cDNA copy is then made though reverse transcription of the viral RNA, a process facilitated by the chaperone CypA and a viral capsid associated reverse transcriptase. (wikipedia.org)
  • simple
  • Additionally, among the 343 unigenes sequences, 22 simple sequence repeats (SSRs) were also identified contributing to the study of A. canescens resources. (mdpi.com)
  • A STS is a unique, simple-copy segment of the genome whose DNA sequence is known and which can be amplified by specific PCR. (biology-online.org)
  • known
  • Once amplified, the sequence can be cut at each end with nucleases and inserted into one of many small circular DNA sequences known as expression vectors. (wikipedia.org)
  • different
  • In particular, non-conservative amino acid substitutions in a highly expressed gut protease may be of adaptive significance for M. persicae feeding on different host plants. (pubmedcentralcanada.ca)
  • development
  • The sequence of these genes encoded all of the information required to specify the reproduction, development, and adult function of an individual organism. (nybg.org)
  • K. Kato developed a highly sensitive method named Adapter-tagged Competitive PCR and applied it to gene expression profiling of mouse postnatal cerebellar development. (nii.ac.jp)