• genes
  • FT belongs to a family of six genes characterized by a phosphatidylethanolamine-binding protein (PEBP) domain. (genetics.org)
  • Brains of mice with conditional knockout of Nfe2l1 in neuronal cells showed decreased proteasome activity and accumulation of ubiquitin-conjugated proteins, and down regulation of genes encoding the 20S core and 19S regulatory sub-complexes of the 26S proteasome. (wikipedia.org)
  • TFs work alone or with other proteins in a complex, by promoting (as an activator), or blocking (as a repressor) the recruitment of RNA polymerase (the enzyme that performs the transcription of genetic information from DNA to RNA) to specific genes. (wikipedia.org)
  • cellular
  • This inherently diverse composition of AP1 complexes and their central role in transcriptional regulation places AP1 complexes at a functional epicenter for pathological signal relay in disease, particularly in the context of malignant cellular transformation in which AP1 proteins are often deregulated by oncoprotein signalling 4 - 6 . (pubmedcentralcanada.ca)
  • Instead, weak promoters, the use of low levels of plasmid DNA in the transfection, and plasmid vectors that do not replicate in mammalian cells should be used to express proteins at or near their endogenous levels to mimic the physiological cellular environment. (wikipedia.org)
  • Also, careful selection of the fluorescent protein is important, as different fluorescent proteins require different cellular environments. (wikipedia.org)
  • C-jun is a proto-oncogene (its protein is Jun) and is the cellular homolog of the viral oncoprotein v-jun. (wikipedia.org)
  • kinase
  • Ubiquitination and degradation of homeodomain-interacting protein kinase 2 by WD40 repeat/SOCS box protein WSB-1. (ebi.ac.uk)
  • This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). (wikipedia.org)
  • The cAMP-regulated enhancer-binding protein ATF-1 activates transcription in response to cAMP-dependent protein kinase A". J. Biol. (wikipedia.org)
  • affinity
  • Most bZIP proteins show high binding affinity for the ACGT motifs, which include CACGTG (G box), GACGTC (C box), TACGTA (A box), AACGTT (T box), and a GCN4 motif, namely TGA(G/C)TCA. (wikipedia.org)
  • bound
  • Structure of the parathyroid hormone receptor C terminus bound to the G-protein dimer Gbeta1gamma2. (ebi.ac.uk)
  • 7. The method of claim 1, wherein the one or more thioaptamers bound to one or more proteins are isolated from a gel. (google.it)
  • 8. The method of claim 1, wherein the one or more thioaptamers are attached to beads and the one or more proteins that have bound the one or more thioaptamers are labeled and the beads are sorted based on protein binding. (google.it)
  • 10. The method of claim 1, wherein the one or more thioaptamers are attached to beads and the step of selecting one or more beads to which one or more proteins has bound is defined further as being fluorescent sorting using a flow-cytometer. (google.it)
  • mechanism
  • Archaeal ubiquitin-like SAMP3 is isopeptide-linked to proteins via a UbaA-dependent mechanism. (nih.gov)
  • The induction of heat-shock protein (HSP) expression is one of the best-characterized responses to high temperature stress, which is similar mechanism of response to high temperatures in all organisms - . (pubmedcentralcanada.ca)
  • enhancer binding
  • They were first described by Landschulz and collaborators in 1988 when they found that an enhancer binding protein had a very characteristic 30-amino acid segment and the display of these amino acid sequences on an idealized alpha helix revealed a periodic repetition of leucine residues at every seventh position over a distance covering eight helical turns. (wikipedia.org)
  • fluorescent
  • Understanding, improving and using green fluorescent proteins. (semanticscholar.org)
  • It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. (wikipedia.org)
  • Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. (wikipedia.org)
  • In 2000, Ghosh et al developed a system that allowed a green fluorescent protein (GFP) to be reassembled using an anti-parallel leucine zipper in E. coli cells. (wikipedia.org)
  • In addition, this study was the first report of an in vivo technique, now known as the bimolecular fluorescence complementation (BiFC) assay, to provide insight into the structural basis of protein complex formation through detection of fluorescence caused by the assembly of fluorescent reporter protein fragments tethered to interacting proteins. (wikipedia.org)
  • First, the fluorescent protein fragments must be able to associate with one another when their tethered proteins interact. (wikipedia.org)
  • 2002
  • This was advanced with the successful reconstitution of the YFP fluorophore from protein fragments that had been fused to interacting proteins within 8 hours of transfection was reported in 2002. (wikipedia.org)
  • mass spectrometry
  • 2. The method of claim 1, further comprising the step of separating the protein into fragments prior to separation by liquid chromatography followed by mass spectrometry. (google.it)
  • 3. The method of claim 1, wherein the steps of identifying the protein by mass spectrometry is preceded by the steps of extracting and separating the proteins by liquid chromatography. (google.it)
  • 4. The method of claim 1, wherein the steps of identifying the protein using mass spectrometry comprises matrix assisted laser desorption ionization mass spectrometry. (google.it)
  • transgenic
  • It was found that overexpression of c-fos from class I MHC promoter in transgenic mice leads to the formation of osteosarcomas due to increased proliferation of osteoblasts whereas ectopic expression of the other Jun and Fos proteins does not induce any malignant tumors. (wikipedia.org)
  • stable
  • However CO protein is stable only in light, so levels stay low throughout short days and are only able to peak at dusk during long days when there is still a little light. (wikipedia.org)
  • promoter
  • This double action could be enabled by differential protein composition of tumour cells and their environment, for example, dimerisation partners, co-activators and promoter architecture. (wikipedia.org)