Bound albumins. Medical search. Frequent questions

Bovine serum albumin is allowed to complex with phenol red dye at various concentration ratios in a series of tests. (diva-portal.org)
Proteins from cell lysate, uncooked serum, eluted serum proteins that were depleted of albumin and IgG, and eluted albumin and IgG from resin, were spiked with the phosphoprotein bovine beta-casein as an internal standard, reduced by 10 mM DTT in the presence of 8 M urea for 30 minutes at 37 C, and then alkylated by 50 mM iodoacetamide at space temp. (scienza-under-18.org)
The effect of bovine serum albumin (BSA) on human liver metabolism, in vitro , of 14 C-phenytoin (PHT) was studied. (aspetjournals.org)
Here, spin labeled salicylic acid (SLSA) binding to and release from bovine serum albumin (BSA) is investigated, as a model for drug-transport protein interaction. (iyte.edu.tr)
It was recently shown that addition of fatty acid free bovine serum albumin (BSA) significantly enhances in vitro activities of UGTs, a limiting factor in in vitro-in vivo extrapolation. (nih.gov)
The objective of the paper is to determine the effect of β-cyclodextrin complexation on the interaction of the popular drug, Atorvastatin to bovine serum albumin. (analchemres.org)
The role of the Atorvastatin-β-cyclodextrin complexation in modulating the binding strength of the drug to the model carrier protein bovine serum albumin is studied using absorption and fluorescence spectral measurements and molecular docking. (analchemres.org)
The results of the binding of the drug to bovine serum albumin in free- and β-cyclodextrin-bound forms. (analchemres.org)
The magnitude of quenching of the fluorescence of bovine serum albumin due to drug binding, an d the Fӧrster energy transfer efficiency between the protein and the drug are decreased in the presence of β-cyclodextrin. (analchemres.org)
Cyclodextrin modulated the binding of drug to the serum albumin, i.e., the cyclodextrin complex of Atorvastatin binds to bovine serum albumin with diminished binding strength. (analchemres.org)
J.H. Shi, K.L. Zhou, Y.Y. Lou, D.Q. Pan, Multi-spectroscopic and molecular modeling approaches to elucidate the binding interaction between bovine serum albumin and darunavir, a HIV protease inhibitor , Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 188 (2018) 362- 371. (ac.rs)
The interactions between selected molecules (piperine, tacrine, curcumin and silibinin) and proteins (acetylcholinesterase and bovine serum albumin) were investigated by Fluorescence spectroscopy, molecular docking, molecular dynamics, free energy calculation and non-covalent interaction analysis. (psgcas.ac.in)
Binding interaction of phosphorus heterocycles with bovine serum albumin: A biochemical study. (omicsdi.org)
Interaction between bovine serum albumin (BSA) and phosphorus heterocycles (PHs) was studied using multi-spectroscopic techniques. (omicsdi.org)
Multi-spectroscopic investigation of the binding interaction of fosfomycin with bovine serum albumin. (omicsdi.org)
The interaction between fosfomycin (FOS) and bovine serum albumin (BSA) has been investigated effectively by multi-spectroscopic techniques under physiological pH 7.4. (omicsdi.org)
Interaction of repaglinide with bovine serum albumin: Spectroscopic and molecular docking approaches. (omicsdi.org)
In view of its biological importance, we have examined the interaction between RPG and a model protein, bovine serum albumin (BSA) employing various spectroscopic, electrochemical and molecular docking methods. (omicsdi.org)
Characterization of the interaction between eupatorin and bovine serum albumin by spectroscopic and molecular modeling methods. (omicsdi.org)
This study investigated the interaction between eupatorin and bovine serum albumin (BSA) using ultraviolet-visible (UV-vis) absorption, fluorescence, synchronous fluorescence, circular dichroism (CD) spectroscopies, and molecular modeling at pH 7.4. (omicsdi.org)
The binding was blocked by preincubation of HBsAg with transglutaminase‐or glutaraldehyde‐cross‐linked human serum albumin, anti‐HBs or monoclonal anti‐pre‐S2, but not by polymerized bovine or rat serum albumin or by monomeric human serum albumin. (mssm.edu)
The presence of bovine serum albumin (BSA) during in vitro assays was recently reported to lower the K m values of both these UGTs for their aglycone substrates without affecting the corresponding V max values. (aspetjournals.org)
Despite sequence identities ranging from 87% to 91% between caprine and bovine caseins, these patients possess IgE antibodies that bind specifically to the caprine caseins without recognizing the bovine ones. (cea.fr)

Albumin-Bound Paclitaxel" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
An injectable formulation of albumin-bound paclitaxel NANOPARTICLES. (umassmed.edu)
This graph shows the total number of publications written about "Albumin-Bound Paclitaxel" by people in this website by year, and whether "Albumin-Bound Paclitaxel" was a major or minor topic of these publications. (umassmed.edu)
Below are the most recent publications written about "Albumin-Bound Paclitaxel" by people in Profiles. (umassmed.edu)
The technology used to create nab-paclitaxel utilizes albumin to deliver paclitaxel, resulting in an advantageous pharmacokinetic (PK) profile. (nih.gov)
This review discusses the proposed mechanism of delivery of nab-paclitaxel, including an examination into a hypothesized greater ability to leverage albumin-based transport relative to sb-paclitaxel. (nih.gov)
An advantageous PK profile and the more efficient use of albumin-based transport may contribute to the preclinical finding that nab-paclitaxel achieves a 33% higher tumor uptake relative to sb-paclitaxel. (nih.gov)
Another possible contributing factor to the tumor accumulation of nab-paclitaxel is the binding of albumin to secreted protein acidic and rich in cysteine (SPARC), although the data supporting this relationship between SPARC and nab-paclitaxel remain largely correlative at this point. (nih.gov)

In this thesis, a 46 amino acid small albumin-binding domain derived from streptococcal protein G was evaluated as a scaffold for the generation of affinity proteins. (avhandlingar.se)
In paper I, a bispecific albumin-binding domain was selected by phage display and utilized as a purification tag for highly efficient affinity purification of fusion proteins. (avhandlingar.se)
The results in paper II show how protein engineering, in vitro display and multi-parameter fluorescence-activated cell sorting can be used to accomplish the challenging task of incorporating two high affinity binding-sites, for albumin and tumor necrosis factor-alpha, into this new bispecific protein scaffold. (avhandlingar.se)
The conformation of the active site of the enzyme is a key determinant of binding affinity ( K m ), so we also wanted to investigate metabolism in an intact cellular system in which the enzyme is maintained as close as possible to the environment in situ . (aspetjournals.org)
These are characterized as a receptor population with high affinity binding (Kd ∼ 17 μM) but relatively low abundance and a separate population with high abundance but much lower affinity (Kd ∼ 364 μM). (lancs.ac.uk)
The low-affinity binding sites are shown to be associated with the yeast cell aggregation. (lancs.ac.uk)
Palmitate was added to citrated plasma in vitro and it was observed that between six and ten palmitate molecules were bound to albumin with nearly equal affinity. (ncl.ac.uk)
The differences may partly be explained by the presence of chloride ions in blood plasma, reducing the affinity for binding of the first two fatty acid molecules, and partly by facilitated binding of several molecules of mixed fatty acids, as found in plasma. (ncl.ac.uk)
S.D. Stojanović, J.M. Nićiforović, S.M. Živanović, J.V. Odović, R.M. Jelić, Spectroscopic studies on the drug-drug interaction: the influence of fluoroquinolones on the affinity of tigecycline to human serum albumin and identification of the binding site , Monatsh. (ac.rs)
The results indicated the high binding affinity of PHs to BSA as it quenches the intrinsic fluorescence of BSA. (omicsdi.org)
The binding constant as calculated from the spectroscopic and voltammetric results was observed to be in the order of 104?M-1?at 298?K, suggesting the moderate binding affinity between RPG and BSA. (omicsdi.org)
This binding site contains a bacterial DAM DNA methylase methylation target sequence which, when methylated, decreases the affinity of the protein for this element. (pasteur.fr)
However, activity was restored in the presence of the upstream elements, showing that cooperation with factors binding to the CCAAT box and distal elements favors the functional interaction of the liver-specific APF/HNF1 factor with lower-affinity binding sites. (pasteur.fr)
Affinity chromatography of albumin fraction isolated by SEHPLC further supported the binding of the carbon-14 label to albumin under in-vivo and in-vitro conditions. (cdc.gov)
SHBG has a high binding affinity to dihydrotestosterone (DHT), medium affinity to testosterone and estradiol, and only a low affinity to estrone, dehydroepiandrosterone (DHEA), androstendione, and estriol. (cdc.gov)
Albumin, which exists in far higher concentrations than SHBG, also binds sexual steroids - although with a clearly lower binding affinity (e.g. about 100 times lower for testosterone). (cdc.gov)

Molecular docking studies indicated that all three complexes primarily bind to HSA in subdomain IIA with amino acid residues such as Arg218 and Lys199 which are located at the entrance of Sudlow's site I. The in vitro cytotoxicities of complexes 1-3 against HeLa cells showed promising anticancer activity (IC 50 = 3.5, 3.9 and 16.9 μM for 1 , 2 and 3 , respectively). (rsc.org)
Protein engineering and in vitro selection systems are powerful methods to generate binding proteins. (avhandlingar.se)
The K m u values determined in the presence of albumin in both microsomes and slices were similar to those based on in vivo human steady state data ( K m u = 2-3 μM), and the intersubject variation, in vitro , was decreased in the presence of BSA. (aspetjournals.org)
Because phenytoin is principally bound to albumin, we also explored the effect of albumin on the V max and K m values obtained, in vitro . (aspetjournals.org)
These studies made it possible to compare V max and K m values obtained in the presence and absence of albumin for two model systems, in vitro , with the V max and K m values observed in vivo . (aspetjournals.org)
is an in vitro blood test that detects changes to the transport and molecular conformation of serum albumin using the method of EPR spectroscopy. (wikipedia.org)
To determine the most prominent features responsible for binding of 20 different flavonoid aglycones to the IIA region of HSA, in vitro fluorescence measurements and density functional theory (DFT) calculations were conducted. (kbco.hr)
In-vitro analysis involved incubation of human plasma or purified albumin with EDB in the presence of phenobarbital treated rat liver microsomes and an NADPH generating system for 2 hours. (cdc.gov)

Azapropazone is primarily bound to the warfarin site, to which also other coumarin derivatives and, e.g., phenytoin and bilirubin are bound. (aspetjournals.org)
Cobinding of each drug with bilirubin was investigated by two techniques, equilibrium dialysis against albumin with and without bilirubin, and by measuring rates of oxidation of free bilirubin with hydrogen peroxide and peroxidase. (aspetjournals.org)
Coronary artery disease (CAD) often oc- to any of the bilirubin forms, including curs in the absence of traditional risk fac- free unconjugated bilirubin, protein-bound tors. (who.int)
Normally, bilirubin bound to serum albumin stays in the intravascular space. (msdmanuals.com)

Human plasma and fatty acid free human albumin were incubated with soman at pH 8.0 and 25°C. Four methods were used to monitor the reaction of albumin with soman: progressive inhibition of the aryl acylamidase activity of albumin, the release of fluoride ion from soman, 31 P NMR, and mass spectrometry. (nebraska.edu)
The ratio of the fractions of bound and unbound spin probes allows estimating the functional activity of albumin molecules. (wikipedia.org)
Analysis of the EPR spectrum allows assessment of the conformation and functional activity of albumin molecules. (wikipedia.org)

Two populations of binding site are identified using two complementary spectroscopic techniques: An extrinsic fluorescent probe, 3-hexa-decanoyl-7-hydrocoumarin ([HEXCO) added to the C. albicans yeast cell surface that records the electrostatic surface potential and so responds to the surface binding of serum albumin and secondly a light scattering technique that reveals how albumin modulates aggregation of the yeast population. (lancs.ac.uk)
Fluorescence and 2D Rotating-frame Overhauser effect spectroscopic techniques are used to determine the stoichiometry, the binding contant, and the mode of binding of Atorvastatin to β-cyclodextrin. (analchemres.org)
Three fluorinated metformin derivatives (MTF1, MTF2 and MTF3) were synthesized and investigated for their human bloodstream - human serum albumin (HSA) interaction studies by multi-spectroscopic techniques (circular dichroism, steady state, time-resolved and synchronous fluorescence), combined with molecular docking and quantum chemical calculation. (amrita.edu)

abstract = "It has been postulated that polymerized human serum albumin may play a role in the infection of hepatocytes by hepatitis B virus, because both the envelope of hepatitis B virus (HBsAg) and hepatocytes exhibit binding activity for human serum albumin after cross‐linking by glutaraldehyde. (mssm.edu)

Ibuprofen, flurbiprofen, and naproxen are primarily bound to the diazepam site on the albumin molecule as shown in interaction studies with albumin immobilized in microparticles. (aspetjournals.org)
b) The stoichiometric description does not imply that preformed binding sites are present in the albumin molecule. (aspetjournals.org)
In combination with knowledge of the protein concentration and the total amount of dye in each case, it is possible to evaluate the number of bound phenol red molecules per protein molecule. (diva-portal.org)
Further, the protein-ligand complexes are subjected to molecular dynamics and binding free energy calculation to confirm the stability of the molecule in the active site of BSA and AChE. (psgcas.ac.in)

This study aimed to investigate the binding properties of TGC to HSA in the presence of QUE, under physiological conditions, by fluorescence spectroscopy. (ac.rs)
G.W. Zhang, Q.M. Que, J.H. Pan, J.B. Guo, Study of the interaction between icariin and human serum albumin by fluorescence spectroscopy , J. Mol. (ac.rs)

Moreover, 2S-albumins contain a protease resistant core which retains most of the allergenicity after digestion. (cea.fr)

Considering the lipophilic characteristics of PPL, its transport through plasma must be assisted by carrier proteins, such as albumin. (nature.com)
Moreover, the native ability of this domain to bind serum albumin provides a useful characteristic that can be used to extend the plasma half-lives of proteins fused to it or potentially of the domain itself. (avhandlingar.se)
The engineered binding proteins generated against the epidermal growth factor receptors 2 and 3 in papers III and IV are aimed in this direction. (avhandlingar.se)
Small bispecific binding proteins based on the albumin-binding domain could potentially contribute to this field. (avhandlingar.se)
These non-immunoglobulin binding proteins may provide several advantages as compared to antibodies in several applications, particularly when a small size and an extended half-life are of key importance. (avhandlingar.se)
Serum proteins, depleted of most albumin and IgG, were recovered by centrifugation. (scienza-under-18.org)
Most of the circulating testosterone is bound to carrier proteins (sex hormone-binding globulin [SHBG], and albumin). (cdc.gov)

Interaction density function method (IDF) indicated that PPL accessed two cooperative sites in RSA, with moderate binding constants (2.3 × 10 5 M −1 and 1.3 × 10 5 M −1 ). (nature.com)
The four antiinflammatory drugs azapropazone, flurbiprofen, ibuprofen, and naproxen all bind very strongly to serum albumin with association constants, K a , of 5.0 x 10 5 , 5.0 x 10 6 , 1.3 x 10 6 , and 1.8 x 10 6 M -1 , respectively. (aspetjournals.org)
The binding constants were determined with albumin immobilized in microparticles and were shown to be in good agreement with those obtained with equilibrium dialysis. (aspetjournals.org)
The results typically show that a total of 13-14 phenol red molecules bind to serumalbumin by two interacting sites with different binding constants. (diva-portal.org)
The binding constants of the PHs obtained from the fluorescence quenching data were found to be decreased with increase of temperature. (omicsdi.org)

FYARRO ® (sirolimus protein-bound particles for injectable suspension) (albumin-bound) is a prescription medicine used to treat adults with malignant perivascular epithelioid cell tumors (PEComa) that have spread to other parts of the body (metastatic) or cannot be removed by surgery (locally advanced, unresectable). (fyarro.com)

Sex hormone-binding globulin (SHBG) is the blood transport protein for androgens and estrogens. (cdc.gov)

Covalent docking simulations and 31 P NMR experiments showed that albumin has no enantiomeric preference for the four stereoisomers of soman. (nebraska.edu)
Spontaneous reactivation at pH 8.0 and 25°C, measured as regaining of aryl acylamidase activity and decrease of covalent adduct (pinacolyl methylphosphonylated albumin) by NMR, occurred at a rate of 0.0044 h -1 , indicating that the adduct is quite stable (t 1/2 = 6.5 days). (nebraska.edu)
At pH 7.4 and 22°C, the covalent soman-albumin adduct, measured by MALDI-TOF mass spectrometry, was more stable (t 1/2 = 20 days). (nebraska.edu)
And, the binding model from molecular docking analysis of both BSA and AChE with these molecules clearly displayed non-covalent interactions (hydrogen bonding and hydrophobic interactions) which played a significant role in the binding mechanism. (psgcas.ac.in)
Covalent binding of ethylene dibromide and its metabolites to albumin. (cdc.gov)

Moreover, we studied drug release and showed that the ratio of bound to unbound SLSA concentrations remains relatively stable during dialysis. (iyte.edu.tr)
This indicates that the binding equilibrium of SLSA is not altered through the process of dialysis. (iyte.edu.tr)
Binding equilibria of long-chain fatty acids to human serum albumin, in serum or plasma, were studied by a dialysis exchange rate technique. (ncl.ac.uk)

Accordingly, it is important to study the interactions of drugs with serum albumin, which determines the pharmacology and pharmacodynamics of drugs. (ac.rs)

63 (13) (2006) 1235-1243.G. Sudlow, D.J. Birkett, D.N. Wade, The Characterization of Two Specific Drug Binding Sites on Human Serum Albumin, Mol. (ac.rs)
11 (1975) 824-832.G. Sudlow, D.J. Birkett, D.N. Wade, Further characterization of specific drug binding sites on human serum albumin , Mol. (ac.rs)

A specific change to the conformation of albumin molecules that is associated with the growth of a malignant tumor is caused by (or associated with) changes in the composition of metabolites carried by serum albumin during the growth of a malignant tumor (proliferating cancer cells uptake and release metabolites in abnormal quantities). (wikipedia.org)

Spin probe molecules bind specifically to albumin molecules where they occupy two main positions: primary binding sites located in albumin domains, where their mobility is restricted the relatively spacious hydrophobic area between the protein domains in the interior of the albumin globule. (wikipedia.org)

We studied SLSA-BSA binding in a wide concentration range and found that the stoichiometry of the drug-protein increases significantly when the physiological range of BSA concentration is reached. (iyte.edu.tr)
R.H. Khan, Interaction of 5-fluoro-5′-deoxyuridine with human serum albumin under physiological and non-physiological condition: A biophysical investigation , Colloid Surf. (ac.rs)

The dye concentrations in the filtrates are determined by spectrophotometry, thus establishing the concentrations of free dye in equilibrium with protein bound dye in each case. (diva-portal.org)

This study demonstrates that cw EPR spectroscopy in combination with spin labeled drugs is an effective technique for binding and release studies and stoichiometric analysis of drug-protein interactions. (iyte.edu.tr)

The antiinflammatory drugs studied have small distribution volumes and low free fractions in plasma, which means that displacement from their binding sites may be of pharmacokinetic significance. (aspetjournals.org)
Though the concentration of albumin in plasma is very high (about 0.6 mM), its reactivity with soman (phosphonylation and phosphotriesterase activity) is too slow to play a major role in detoxification of the highly toxic organophosphorus compound soman. (nebraska.edu)
Since glutaraldehyde‐dependent cross‐linking of albumin molecules is not likely to occur in vivo, we considered the possibility that albumin may be polymerized by the action of transglutaminase enzymes present in plasma as activated factor XIII or released into plasma from tissues. (mssm.edu)
During riboflavin localization in plasma, it is bound to albumin and specific immunoglobulins. (sigmaaldrich.com)

After absorption they bind to human serum albumin (HSA), the most abundant carrier protein in the blood which also binds various hormones and drugs. (kbco.hr)

These binding characteristics are of huge interest for understanding pharmacokinetic mechanism of the target molecules. (psgcas.ac.in)

CCAAT/enhancer binding protein activates the promoter of the serum albumin gene in cultured hepatoma cells. (johnshopkins.edu)
An expression vector capable of encoding full-length CCAAT/enhancer-binding protein (C/EBP) has been constructed and tested in transient transfection assays for its capacity to activate transcription from the promoter of the serum albumin gene. (johnshopkins.edu)
When tested in cultured hepatoma cells, the C/EBP expression vector achieved potent trans-activation of the albumin promoter. (johnshopkins.edu)
Expression vectors that encoded defective forms of C/EBP failed to activate the albumin promoter. (johnshopkins.edu)
Dive into the research topics of 'CCAAT/enhancer binding protein activates the promoter of the serum albumin gene in cultured hepatoma cells. (johnshopkins.edu)
9:4750-4758, 1989) six different elements in the albumin promoter. (pasteur.fr)
When the different albumin promoter constructions were prepared in an Escherichia coli deoxyadenosine methylase-negative strain, the respective contributions of the elements to the overall promoter activity were strikingly different. (pasteur.fr)

Domatinostat tosylate The kit consists of spin columns and immobilized monoclonal antibodies for depletion of albumin and IgG. (scienza-under-18.org)

The stoichiometric description is preferred for the following reasons: (a) Simple relations exist between the percentage of bound drug at low drug concentrations and the first stoichiometric binding constant. (aspetjournals.org)

From this study, a new drug binding subsite on HSA Sudlow site 1 was identified. (rcsb.org)
Zhu, F. Yang, L. Chen, E.J. Meehan, M. Huang, A new drug binding subsite on human serum albumin and drug-drug interaction studied by X-ray crystallography , J. Struct. (ac.rs)

The ability to track drug binding and release makes electron paramagnetic resonance (EPR) spectroscopy well suited for drug delivery studies. (iyte.edu.tr)

Albumin binding of anti-inflammatory drugs. (aspetjournals.org)
Using the continuous wave (cw) EPR technique to extract information about the dynamics of the spin labeled drugs we can simultaneously determine the bound and unbound drugs. (iyte.edu.tr)
Binding of a drug to HSA often influences simultaneous binding of other drugs. (rcsb.org)
The metabolism of drugs, their distribution, free concentration, and efficacy depend on the drug-serum albumin interaction [1]. (ac.rs)

C(H)-C 6 H 4 OH- o , o -phen = 1,10-phenanthroline, and OAc = CH 3 COO − ) with human serum albumin (HSA) was studied using fluorescence quenching, synchronous and 3D fluorescence measurements and UV-vis spectroscopy. (rsc.org)
The test is based on the interaction of spin-labeled fatty acids with serum albumin by means of EPR spectroscopy. (wikipedia.org)
Electron Spin Resonance Spectroscopy of Albumin Transport Quality Is a New Test for Diagnosis of Kidney Transplant Acute Rejection. (wikipedia.org)

The basis of hapten- molecular mechanisms of the sensitization pro- protein binding work is the hypothesis that upon cess will result in novel opportunities for the skin absorption, only protein-reactive chemicals development of alternative methods for assessing (or those that can be metabolically or chemically skin sensitization hazard and relative potency of converted to protein-reactive species) are able to chemicals. (cdc.gov)

We showed that heating of 2S albumins in solution induced their denaturation and their aggregation and thereby reduced significantly the IgE-binding capacity and the elicitation potency (FP7 Europrevall program). (cea.fr)

These findings for phenytoin metabolism suggest that the addition of albumin to incubation media for slices or microsome experiments may yield K m estimates that are more representative of in vivo values. (aspetjournals.org)

Both steric and electronic characteristics of flavonoids have a great impact on their binding to HSA, with hydrogen donor and acceptor properties and coplanarity being the most prominent. (kbco.hr)

S.D. Stojanovic, S.M. Jankovic, Z.D. Matovic, I.Z. Jakovljevic, R.M. Jelic, Interaction between tigecycline and human serum albumin in aqueous solution, Monatsh Chem 146 (2015) 399-409. (ac.rs)

The binding constant value of the drug-protein binding in the absence and presence of β-cyclodextrin are 5.36 × 104 M-1 to 2.32 × 104 M-1 respectively. (analchemres.org)

2016) 'Structural and Electronic Determinants of Flavonoid Binding to Human Serum Albumin: an Extensive Ligand-based Study', Royal Society of Chemistry , 6, str. (kbco.hr)

Although PPL is present in the bloodstream, no information is found on the interaction between PPL and rat serum albumin (RSA), the most abundant protein with the function of transporting endo/exogenous molecules. (nature.com)

Reduced functional activity of serum albumin (reduced binding efficacy) is associated with toxemia (an increase in the concentration of toxic molecules in the blood) and is manifested before other clinical symptoms. (wikipedia.org)

Binding of flavonoids to HSA may impact their distribution, influencing the active concentration in the blood. (kbco.hr)

The albumin and IgG in the sample, which were bound by antibodies immobilized on a solid support in the spin column, were mixed with 8 M urea and then recovered by centrifugation. (scienza-under-18.org)

The albumin binding sites are found to possess different binding affinities and relative abundance leading to different total binding capacities. (lancs.ac.uk)
The number of binding sites n and observed binding constant KA were measured by the fluorescence quenching method. (omicsdi.org)
These data indicate that HBsAg particles contain specific binding sites for transglutaminase‐cross‐linked human serum albumin, but it remains to be determined whether the albumin polymers play a role in the attachment of hepatitis B virus to hepatocytes. (mssm.edu)

HBsAg particles bound transglutaminase‐cross‐linked as well as glutaralde‐hyde‐cross‐linked human serum albumin as demonstrated by radioimmunoassay and immunoelectron microscopy. (mssm.edu)

Anatomy16

Organisms10

Diseases16

Chemicals and Drugs108

Analytical, Diagnostic and Therapeutic Techniques and Equipment50

Phenomena and Processes58

Disciplines and Occupations2

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Bovine23

Paclitaxel8

Affinity16

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Subsite2

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