Bound myosins. Medical search. Frequent questions

MYBPC3 was thus the fourth gene for hypertrophic cardiomyopathy, following MYH7, encoding β-myosin heavy chain, TNNT2 and TPM1, encoding cardiac troponin T and α-tropomyosin, respectively, earmarking hypertrophic cardiomyopathy as a disease of the sarcomere. (wikipedia.org)
Mutations in five different loci cause FHC and 3 disease genes have been identified: beta cardiac myosin heavy chain, alpha tropomyosin and cardiac troponin T. Because these genes encode contractile proteins, other FHC loci are predicted also to encode sarcomere components. (ox.ac.uk)
The regulation of vertebrate striated muscle contraction involves a number of different molecules, including the thin-filament accessory proteins tropomyosin and troponin that provide Ca(2+)-dependent regulation by controlling access to myosin binding sites on actin. (egelmanlab.org)
One position aligns well with the previously reported binding site that clashes with the binding of myosin to actin, but would force tropomyosin into an "on" position that exposes myosin binding sites along the filament. (egelmanlab.org)
The second position identified here would not interfere with either myosin binding or tropomyosin positioning. (egelmanlab.org)
It thus appears that the ability to bind to at least two distinctly different positions on F-actin, as observed for tropomyosin, may be more common than previously considered for other actin binding proteins. (egelmanlab.org)
The backbone of the sarcomere is composed of three filament systems: the myosin-based thick filament, the actin-based thin filament, supplemented with the regulatory protein tropomyosin and the troponin complex, and the titin filament. (frontiersin.org)
Intermicrovillous space increases with contractile activity of myosin II and tropomyosin , and decreases when contraction ceases. (wikipedia.org)
Three types of troponins exist-troponin I, troponin T, and troponin C. Each subunit has a unique function: Troponin T binds the troponin components to tropomyosin, troponin I inhibits the interaction of myosin with actin, and troponin C contains the binding sites for Ca2+ that helps initiate contraction. (medscape.com)

The measured FRET efficiencies were intermediate between those observed when the donor was attached to the cardiac myosin regulatory light chain in the thick filaments and troponin T in the thin filaments. (kcl.ac.uk)
Cardiac MyBP-C is arrayed transversely in sarcomere A-bands and binds myosin heavy chain in thick filaments and titin in elastic filaments. (ox.ac.uk)
Each sarcomere consists of thick filaments of myosin, shown in purple, and thin filaments of actin, shown in orange. (pearson.com)
A sarcomere contracts when its actin filaments slide past its myosin filaments. (pearson.com)
Contraction shortens the sarcomere but does not change the length of the actin or myosin filaments. (pearson.com)
The myosin filaments have heads that bind and pull actin repeatedly, shortening the sarcomere. (pearson.com)
The plus ends of the actin filaments are located at the tip of the microvillus and are capped, possibly by capZ proteins, [2] while the minus ends are anchored in the terminal web composed of a complicated set of proteins including spectrin and myosin II. (wikipedia.org)
Titin activates myosin filaments in skeletal muscle by switching from an extensible spring to a mechanical rectifier. (unifi.it)
The motor domain at the N-terminus of the myosin heavy chain binds actin filaments in an ATP-dependent manner. (elifesciences.org)
The C-terminal tail of the myosin heavy chain associates with the tails of other myosin heavy chains and promotes the assembly into bipolar filaments. (elifesciences.org)
The bipolar myosin filaments promote the sliding of antiparallel actin filaments relative to one another resulting in contraction of an actin network. (elifesciences.org)
The rational for the different myosin fragments lies in the different biochemical properties: Full-length myosin forms filaments, sediments at high speed and can be used in the in vitro motility assay. (elifesciences.org)

In addition to myosin, cMyBP-C also binds titin and actin. (wikipedia.org)
MyBP-C contributes to thick filament structure via interactions at its C-terminus with the light meromyosin section of the myosin rod and with titin. (ox.ac.uk)
Myosin binding protein C interacts with other muscle proteins, including myosin, actin, and titin. (medlineplus.gov)

One possibility is that because the N-terminus contains a unique binding site for the S2 subfragment of myosin, shortened cMyBP-C peptides could directly affect myosin contraction by binding to S2. (arizona.edu)
Cardiac myosin binding protein-C (cMyBP-C) is definitely a solid filament-associated protein that Mitragynine appears to contribute to the regulation of cardiac contraction through interactions with either myosin or actin or both. (biopaqc.com)
Its central position in the sarcomere and the tight association to myosin are the basis for titin's role in maintaining the structural integrity of the sarcomere during the relaxation-contraction cycle. (frontiersin.org)
Mixing ground beef releases myosin, which is a protein that causes muscle contraction. (tastingtable.com)
Fast skeletal myosin binding protein-C modulates the speed and force of fast skeletal muscle contraction. (uc.edu)
Our study concludes that fast myosin binding protein-C is essential in regulating the force generation and speed of contraction of fast muscles. (uc.edu)
Matching Mechanics and Energetics of Muscle Contraction Suggests Unconventional Chemomechanical Coupling during the Actin-Myosin Interaction. (unifi.it)
Although, the non-muscle myosin II holoenzyme (myosin) is a molecular motor that powers contraction of actin cytoskeleton networks, recent studies have questioned the importance of myosin motor activity cell and tissue shape changes. (elifesciences.org)
Overall, our data highlights that myosin activity is required for rapid cell contraction and tissue folding in developing Drosophila embryos. (elifesciences.org)
Forces that shape cells and tissues can be produced by the contraction of actin filament (F-actin) meshworks by the molecular motor Myosin II (myosin). (nature.com)

Barefield, D. and Sadayappan, S. (2010) Phosphorylation and function of cardiac myosin-binding protein c in health and disease. (scirp.org)
Moreover, stimulation of NRCs with β-adrenergic agonists reduces FRET between NcMyBP-C and actin-bound Phalloidin, suggesting that cMyBP-C phosphorylation reduces its interaction with the thin filament. (kcl.ac.uk)
These results demonstrate that binding of the C1C2 peptide to S2 alone is sufficient to affect myosin contractile function and suggest that regulated binding of cMyBP-C to myosin S2 by phosphorylation directly influences myofilament Ca 2+ sensitivity. (arizona.edu)
Cardiac Myosin Binding Protein-C (cMyBPC) and cardiac troponin I (cTNI) are hypertrophic cardiomyopathy (HCM)-causing sarcomeric proteins which regulate contractility in response to PKA phosphorylation. (biomedcentral.com)
Work done using smooth muscle myosin and mammalian non-muscle myosin have demonstrated that phosphorylation of the RLC at conserved Serine and Threonine sites ( Figure 1B , Serine-19 and Threonine-18) activates myosin motor activity, enhances the affinity of myosin for actin, and promotes myosin filament assembly ( Heissler and Sellers, 2016 ). (elifesciences.org)
However, it has not been biochemically demonstrated that Drosophila myosin motor activity and filament assembly is regulated by RLC phosphorylation or whether the extent of activation is similar to that of mammalian systems. (elifesciences.org)

In its dephosphorylated state, cMyBP-C binds predominantly to myosin S2 and brakes crossbridge formation, however, when phosphorylated in response to β-adrenergic stimulation through activating cAMP-dependent protein kinase (PKA), it favours binding to actin, then accelerating crossbridge formation, enhancing force development and promoting relaxation. (wikipedia.org)

Mutations in the gene for cardiac myosin-binding protein C account for approximately 15 percent of cases of familial hypertrophic cardiomyopathy. (nih.gov)
DNA sequences encoding cardiac myosin-binding protein C were determined in unrelated patients with familial hypertrophic cardiomyopathy. (nih.gov)
eight defects (insertions, deletions, and splice mutations) were predicted to truncate cardiac myosin-binding protein C. The clinical expression of either missense or truncation mutations was similar to that observed for other genetic causes of hypertrophic cardiomyopathy, but the age at onset of the disease differed markedly. (nih.gov)
Schlossarek, S., Mearini, G. and Carrier, L. (2011) Cardiac myosin-binding protein c in hypertrophic cardiomyopathy: Mechanisms and therapeutic opportunities. (scirp.org)
Cardiac myosin binding protein-C (cMyBP-C) is a thick filament-associated regulatory protein frequently found mutated in patients suffering from hypertrophic cardiomyopathy (HCM). (kcl.ac.uk)
Mutations in the cardiac myosin binding protein-C gene on chromosome 11 cause familial hypertrophic cardiomyopathy. (ox.ac.uk)
Mutations in the cardiac myosin binding protein-C gene (cMyBP-C) are among the most prevalent causes of inherited hypertrophic cardiomyopathy. (arizona.edu)
In contrast hypertrophic cardiomyopathy mutations in cMyBP-C that are associated with hypercontractility actually in an individual at rest presumably induce hypercontractility by disrupting the relationships of cMyBP-C with myosin or increasing its binding to actin or both. (biopaqc.com)

The vanadate (Vi)-mediated photocleavage reaction was used to study the interaction between the regulatory segment and the catalytic site of smooth muscle myosin light chain kinase (MLCK). (go.jp)

The myosin-binding protein C, cardiac-type is a protein that in humans is encoded by the MYBPC3 gene. (wikipedia.org)
Mitalipov and his colleagues have convincingly repaired embryos carrying the faulty gene, cardiac myosin-binding protein C (MYBPC3). (cosmosmagazine.com)

Cardiac myosin binding protein-C (cMyBP-C) is one of the proteins that make up the contractile apparatus of cardiomyocytes. (scirp.org)
In vitro studies showed that ligation of genetically encoded fluorophores to NcMyBP-C had no or little effect on its binding to thick and thin filament proteins. (kcl.ac.uk)
Ackermann MA, Kontrogianni-Konstantopoulos A. Myosin binding protein-C slow: an intricate subfamily of proteins. (medlineplus.gov)

cMyBP-C is a myosin-associated protein that binds at 43 nm intervals along the myosin thick filament backbone, stretching for 200 nm on either side of the M-line within the crossbridge-bearing zone (C-region) of the A band in striated muscle. (wikipedia.org)
The approximate stoichiometry of cMyBP-C along the thick filament is 1 per 9-10 myosin molecules, or 37 cMyBP-C molecules per thick filament. (wikipedia.org)
These results are consistent with the coexistence of multiple conformations of cMyBP-C, some with their N-terminal domains binding to the thin filament and others binding to the thick filament, supporting the hypothesis that the dynamic interchange between these conformations mediates interfilament signaling in the regulation of contractility. (kcl.ac.uk)
Myosin binding protein-C (MyBP-C) is a thick filament-associated protein localized to the crossbridge-containing C zones of striated muscle sarcomeres. (ox.ac.uk)
Several structural models of the interaction of MyBP-C with myosin have been proposed, although its precise arrangement on the thick filament remains to be elucidated. (ox.ac.uk)
Myosin-binding protein-C is a thick filament regulatory protein found in striated muscle in both the heart and skeletal system. (uc.edu)

The genotypes of these family members were determined, and the clinical status of 212 family members with mutations in the gene for cardiac myosin-binding protein C was assessed. (nih.gov)
The clinical expression of mutations in the gene for cardiac myosin-binding protein C is often delayed until middle age or old age. (nih.gov)
Delayed expression of cardiac hypertrophy and a favorable clinical course may hinder recognition of the heritable nature of mutations in the cardiac myosin-binding protein C gene. (nih.gov)
This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. (nih.gov)
The gene encoding the cardiac isoform of myosin binding protein-C (cardiac MyBP-C) has recently been assigned to chromosome 11p11.2 and proposed as a candidate FHC gene. (ox.ac.uk)
Myosin binding protein C1: a novel gene for autosomal dominant distal arthrogryposis type 1. (medlineplus.gov)
Song says that research examined the role of the protein in fast-twitch muscles by generating a knockout mouse - an animal in which researchers have either inactivated, replaced or disrupted the existing fast myosin binding protein-C gene to study its impact. (uc.edu)
The MYO15A gene is located at chromosome 17p11.2 and contains 66 coding exons, which encodes an unconventional myosin protein Myosin XVA [ 19 ]. (hindawi.com)

cMyBP-C regulates the positioning of myosin and actin for interaction and acts as a tether to the myosin S1 heads, limiting their mobility. (wikipedia.org)
A nine-amino-acid extension at the C-terminal helix (Helix2) of MyUb is required for myosin VI interaction with endocytic and autophagic adaptors. (uniroma3.it)
This work provides fundamental insights into myosin VI interaction with ubiquitinated cargo and functional adaptors. (uniroma3.it)
Myosin VI interaction with T6BP and NDP52 was confirmed in vitro and in vivo and the binding sites on each protein were accurately mapped. (ox.ac.uk)

The force of the myosin motor sets cooperativity in thin filament activation of skeletal muscles. (unifi.it)

Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. (nih.gov)
We identify a structured domain in myosin VI, myosin VI ubiquitin-binding domain (MyUb), that binds to ubiquitin chains, especially those linked via K63, K11, and K29. (uniroma3.it)
In addition, we found that an isoform-specific helix restricts MyUb binding to ubiquitin chains. (uniroma3.it)
Myosin is a hexamer composed of two myosin heavy chains, two regulatory light chains (RLCs), and two essential light chains (ELCs) ( Figure 1A ). (elifesciences.org)
The light chains bind to the central neck domain of the myosin heavy chain and have structural and regulatory functions ( Heissler and Sellers, 2014 ). (elifesciences.org)
The top panel shows the myosin hexamer composed of two myosin heavy chains (green), two ELCs (light blue) and two RLCs (gray). (elifesciences.org)

Myosin XVA is a large actin-based motor protein. (hindawi.com)

Cardiac myosin binding protein C (cMyBP-C) appears to modulate this Ca(2+)-dependent regulation and has attracted increasing interest due to links with inherited cardiac diseases. (egelmanlab.org)
These observations help to explain many of the seemingly contradictory results obtained with cMyBP-C and show how cMyBP-C can provide an additional layer of regulation to actin-myosin interactions. (egelmanlab.org)
The protein, fast skeletal myosin binding protein-C (fMyBP-C), plays a foundational role in the proper regulation of contractile structure and function in the body's fast twitch muscles - these muscles produce sudden bursts of power to sprint into action, jump or lift heavy objects. (uc.edu)

Both mutations are predicted to disrupt the high affinity, C-terminal, myosin-binding domain of cardiac MyBP-C. These findings define cardiac MyBP-C mutations as the cause of FHC on chromosome 11p and reaffirm that FHC is a disease of the sarcomere. (ox.ac.uk)

derived from the calmodulin binding site of skeletal muscle MLCK. (tocris.com)
In the enterocyte microvillus, the structural core is attached to the plasma membrane along its length by lateral arms made of myosin 1a and Ca 2+ binding protein calmodulin . (wikipedia.org)

Interactions of cMyBP-C with its binding partners vary with its posttranslational modification status. (wikipedia.org)
Thus myosin VI-T6BP interactions may link membrane trafficking pathways with cell adhesion and cytokine-dependent cell signalling. (ox.ac.uk)

Myosin 1a functions through a binding site for filamentous actin on one end and a lipid binding domain on the other. (wikipedia.org)

The defect in the myosin motor activity in these mutants is evident in developing Drosophila embryos where tissue recoil following laser ablation is decreased compared to wild-type tissue. (elifesciences.org)
In the Drosophila thorax, opposing gradients of Dachsous and Four-jointed expression, which are constituents of the Fat/Dachsous/Four-jointed planar cell polarity pathway, result in the polarized localization of the myosin Dachs 9 . (nature.com)

The protein product of MYH16 is one of the heavy chain myosins, a kind of protein that works with actin to enable muscle fibers to contract. (johnhawks.net)
The protein is denoted as MyHC-M, for myosin heavy chain-masticatory. (johnhawks.net)
A ) Domain organization of the myosin heavy chain and myosin fragments used to study the biochemical properties of myosin. (elifesciences.org)
The myosin motor domain, the light chain binding neck and the tail domain of the heavy chain are indicated. (elifesciences.org)

The slow isoform of myosin binding protein C is active during the development of skeletal muscles. (medlineplus.gov)
The mutation changes a single protein building block (amino acid) in the slow isoform of myosin binding protein C. Specifically, the mutation replaces the amino acid tryptophan with the amino acid arginine at protein position 236 (written as Trp236Arg or W236R). (medlineplus.gov)

This release of calcium initiates actin-myosin crossbridge activity and the observation of the muscle shortening and contracting. (jove.com)

Myosin VI has been implicated in many cellular processes including endocytosis, secretion, membrane ruffling and cell motility. (ox.ac.uk)

This results in a decreased number of crossbridges formed, which hinders force generation, due to its N-terminal C1-M-C2 region interacting with the myosin-S2 domain. (wikipedia.org)
Furthermore, GSK3β was described as another protein kinase to phosphorylate cMyBP-C outside the M-domain in the proline-alanine-rich actin-binding site at Ser133 in human myocardium (mouse Ser131). (wikipedia.org)
Complete sequence of human fast-type and slow-type muscle myosin-binding-protein C (MyBP-C). Differential expression, conserved domain structure and chromosome assignment. (medlineplus.gov)
Myosin XVA through its carboxy-terminal PDZ-ligand interacts with the third PDZ domain of whirlin, and then delivers whirlin to the tips of stereocilia [ 21 ]. (hindawi.com)

For that action, fast muscle is essential, and fast myosin binding protein-C is the key molecule to regulate the speed of action. (uc.edu)
We also think if we can manipulate the workings of fast myosin binding protein-C in skeletal muscle that we can prevent or at least slow down the loss of muscle function in genetic muscle disease such as distal arthrogryposis. (uc.edu)
There, the motor neuron establishes synaptic contact with the muscle fiber and triggers the release of the neuro transmitter acetylcholine, which diffuses across the synaptic cleft and binds to receptors. (jove.com)
Binding of acetylcholine to its receptors on the sarcolemma allows entry of sodium ions into the cell and triggers an action potential in the muscle cell. (jove.com)
Anisotropic Elasticity of the Myosin Motor in Muscle. (unifi.it)
Myosin motors that cannot bind actin leave their folded OFF state on activation of skeletal muscle. (unifi.it)

These results suggest that the three-dimensional structure of MLCK brings the regulatory segment into direct contact with the ATP-binding site. (go.jp)
Moreover, the binding of Ca 2+ -CaM displaces the regulatory segment away from the catalytic site. (go.jp)

Here, combining the biochemical analysis of enzymatic and motile properties for purified myosin mutants with in vivo measurements of apical constriction for the same mutants, we show that in vivo constriction rate scales with myosin motor activity. (elifesciences.org)

Song says advancing the knowledge of fast myosin binding protein-C may someday assist in addressing skeletal muscular disorders. (uc.edu)

In this regard there is evidence (examined below) that dephosphorylated cMyBP-C preferentially binds to myosin and in so doing restricts spatial mobility of myosin and reduces the probability of myosin binding to actin. (biopaqc.com)

A number of single amino acid mutations linked to clinical diseases occur in the N-terminal region of cMyBP-C, including domains C0 and C1, which previously have been shown to bind to F-actin. (egelmanlab.org)

Studies suggest that myosin binding protein C contributes to the stability and maintenance of sarcomeres. (medlineplus.gov)

Analysis of the amino acid composition of the fragment revealed that the cleavage site at 28 kDa from C-terminus occur red at Lys 799±3 amino acid residues, which is in a region where the CaM-binding and pseudosubstrate regions overlap. (go.jp)

We carried out a yeast two-hybrid screen and identified TRAF6-binding protein (T6BP) and nuclear dot protein 52 (NDP52) as myosin VI binding partners. (ox.ac.uk)

Anatomy15

Organisms9

Chemicals and Drugs56

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Phenomena and Processes23

Information Science3

Health Care1

Tropomyosin9

Filaments12

Titin3

Contraction10

Phosphorylation6

CMyBP-C binds1

Hypertrophic cardiomyopathy8

Smooth muscle myosin1

MYBPC32

Proteins3

Thick6

Gene8

Interaction4

Filament1

Chains6

Actin-based1

Regulation3

Sarcomere1

Calmodulin2

Interactions2

Lipid1

Drosophila2

Heavy4

Isoform2

Crossbridge1

Membrane1

Domain4

Muscle6

Regulatory2

Constriction1

Skeletal1

Restricts1

Mutations1

Contributes1

Pseudosubstrate1

Nuclear1