Loading...


  • HMGA1
  • We first show that HMGA1 is needed for basal and cAMP-induced retinol-binding protein 4 (RBP4) gene and protein expression in living cells of both human and mouse origin. (nih.gov)
  • In comparative studies of normal and mutant mice, glucagon administration caused a considerable upregulation of HMGA1 and RBP4 expression both at the mRNA and protein level in wild-type animals. (nih.gov)
  • Conversely, in Hmga1-knockout mice, basal and glucagon-mediated expression of RBP4 was severely attenuated and correlated inversely with increased Glut4 mRNA and protein abundance in skeletal muscle and fat, in which the activation state of the protein kinase Akt, an important downstream mediator of the metabolic effects of insulin on Glut4 translocation and carbohydrate metabolism, was simultaneously increased. (nih.gov)
  • High-mobility group protein HMG-I/HMG-Y is a protein that in humans is encoded by the HMGA1 gene. (wikipedia.org)
  • HMGA1 proteins are quite small (~10-12 kDa) and basic molecules, and consist of three AT-hooks with the RGRP (Arg-Gly-Arg-Pro) core motif, a novel cross-linking domain located between the second and third AT-hook, and a C-terminal acidic tail characteristic for the HMG family comprising HMGA, HMGB and HMGN proteins. (wikipedia.org)
  • HMGA1-GFP fusion proteins are highly dynamic in vivo (determined using FRAP analysis), but in contrast also show nanomolar affinity to AT-rich DNA in vitro (determined biochemically), which might be explained due to the extensive post-transcriptional modifications in vivo. (wikipedia.org)
  • HMGA1 preferentially binds to the minor groove of AT-rich regions in double-stranded DNA using its AT-hooks. (wikipedia.org)
  • HMGA1 proteins have high amounts of diverse posttranslational modifications and are located mainly in the nucleus, especially in heterochromatin, but also in mitochondria and the cytoplasm. (wikipedia.org)
  • High mobility group A (HMGA1) is an architectural transcription factor that encodes a nonhistone chromatin protein. (biomedcentral.com)
  • HMGA1 Full-Length MS Protein Standard (NP_002122), Labeled with [U- 13C6, 15NL-Arginine and [U- 13C6, 15NL-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. (creative-proteomics.com)
  • mRNA
  • As a result, these mRNA molecules are silenced, by one or more of the following processes: Cleavage of the mRNA strand into two pieces, Destabilization of the mRNA through shortening of its poly(A) tail, and Less efficient translation of the mRNA into proteins by ribosomes. (wikipedia.org)
  • isolated the lin-4 gene, they found that instead of producing an mRNA encoding a protein, it produced short non-coding RNAs, one of which was a ~22-nucleotide RNA that contained sequences partially complementary to multiple sequences in the 3' UTR of the lin-14 mRNA. (wikipedia.org)
  • This complementarity was proposed to inhibit the translation of the lin-14 mRNA into the LIN-14 protein. (wikipedia.org)
  • It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. (uniprot.org)
  • The protein appears to have important roles in neuronal development and mRNA transport. (wikipedia.org)
  • Human RAGE mRNA undergoes alternative splicing, much as with other proteins located within the MHC-III locus on chromosome 6. (biomedcentral.com)
  • HMGA2
  • Using transgenic mouse technology, they also showed (concurrently and independently of Alfredo Fusco), that fusion proteins between HMGA2 and other C terminal peptides (following chromosomal translocation) can drive the development of lipomas and generate obese mice. (wikipedia.org)
  • Subsequently, we generated an adenovirus containing the HMGA2 gene in the antisense orientation (Ad-A2as) to study the effect of HMGA2 protein suppression in ALT cells. (aacrjournals.org)
  • On the basis of these findings, the targeting of the HMGA2 protein expression may represent a promising approach for treating the well-differentiated liposarcomas resistant to conventional therapies. (aacrjournals.org)
  • gene
  • Since these signals are often unknown, the in vitro microarray based method described in this video article can be used to determine gene targets and binding sites for response regulators. (jove.com)
  • The gene from A. fulgidus codes for a protein of 40 kDa monomeric molecular weight, which we overexpressed in Escherichia coli and purified to homogeneity. (jove.com)
  • This gene encodes a non-histone chromatin protein involved in many cellular processes, including regulation of inducible gene transcription, DNA replication, heterochromatin organization, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. (wikipedia.org)
  • roles
  • The candidate sequences presented here represent important information for research into the various roles of HMGA1a, including cell differentiation, death, growth, proliferation, and the pathogenesis of cancer. (jove.com)
  • His research at UCLA has focused on roles of novel protein methyltransferases in aging and biological regulation highlighted by discoveries of the protein L-isoaspartyl repair methyltransferase, the isoprenylcysteine protein methyltransferase, and the protein phosphatase 2A methyltransferase. (ucla.edu)
  • Possible roles of this new AAA+ protein are discussed. (jove.com)
  • sequence
  • However, in most other organisms, ORC lacks sequence‐specific DNA‐binding activity. (embopress.org)
  • Schizosaccharomyces pombe ORC lacks sequence‐specific binding, but the spORC4 subunit possesses a species‐specific AT‐hook domain that confers non‐specific binding to AT‐rich DNA ( Chuang and Kelly, 1999 ). (embopress.org)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • cellular
  • Pubmed ID: 16584891 We investigated a new archaeal member of the AAA+ protein family (ATPases associated with various cellular activities) which is found in all methanogenic archaea and the sulphate-reducer Archaeoglobus fulgidus. (jove.com)
  • metabolism
  • It is found either as a membrane-bound or soluble protein that is markedly upregulated by stress in epithelial cells, thereby regulating their metabolism and enhancing their central barrier functionality. (biomedcentral.com)
  • metastatic
  • Moreover, S100A13 was found to be involved in the invasiveness of lung cancer cell lines [ 10 ], and also was detected in tumor cells circulating in blood of patients with metastatic cancer [ 11 ], indicating that this protein may be considered as a predictor of cancer metastases. (biomedcentral.com)
  • Molecular
  • He obtained his PhD in Biochemistry and Molecular Biology at Harvard University working as an NSF Fellow with Professor Guido Guidotti on membrane protein-detergent interactions and the identification of the major rat liver mitochondrial polypeptides as enzymes of the urea cycle. (ucla.edu)
  • structural
  • Characterization of a New AAA+ Protein from Archaea Journal of Structural Biology. (jove.com)
  • The protein is most homologous to the AAA-domain of FtsH from bacteria, while the N-terminal domain shows predicted structural homology to members of the CDC48 family of AAA proteins. (jove.com)
  • complex
  • As suggested by homology to other members of the AAA+ family, the complex binds and hydrolyzes ATP. (jove.com)
  • different
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • domain
  • HMGA1a, which can functionally substitute for LR1 and LR2 domain, can also recruit ORC in an RNA‐dependent manner. (embopress.org)
  • p>This subsection of the 'Function' section specifies the position and type of each DNA-binding domain present within the protein. (uniprot.org)
  • RAGE is expressed as both full-length, membrane-bound forms (fl-RAGE or mRAGE, not to be confused with mouse RAGE) and various soluble forms lacking the transmembrane domain. (biomedcentral.com)
  • given
  • be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein. (uniprot.org)
  • HMGA1
  • We first show that HMGA1 is needed for basal and cAMP-induced retinol-binding protein 4 (RBP4) gene and protein expression in living cells of both human and mouse origin. (nih.gov)
  • In comparative studies of normal and mutant mice, glucagon administration caused a considerable upregulation of HMGA1 and RBP4 expression both at the mRNA and protein level in wild-type animals. (nih.gov)
  • Conversely, in Hmga1-knockout mice, basal and glucagon-mediated expression of RBP4 was severely attenuated and correlated inversely with increased Glut4 mRNA and protein abundance in skeletal muscle and fat, in which the activation state of the protein kinase Akt, an important downstream mediator of the metabolic effects of insulin on Glut4 translocation and carbohydrate metabolism, was simultaneously increased. (nih.gov)
  • HMGA1 Full-Length MS Protein Standard (NP_002122), Labeled with [U- 13C6, 15NL-Arginine and [U- 13C6, 15NL-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. (creative-proteomics.com)
  • High mobility group A (HMGA1) is an architectural transcription factor that encodes a nonhistone chromatin protein. (biomedcentral.com)
  • High-mobility group protein HMG-I/HMG-Y is a protein that in humans is encoded by the HMGA1 gene. (wikipedia.org)
  • HMGA1 proteins are quite small (~10-12 kDa) and basic molecules, and consist of three AT-hooks with the RGRP (Arg-Gly-Arg-Pro) core motif, a novel cross-linking domain located between the second and third AT-hook, and a C-terminal acidic tail characteristic for the HMG family comprising HMGA, HMGB and HMGN proteins. (wikipedia.org)
  • HMGA1-GFP fusion proteins are highly dynamic in vivo (determined using FRAP analysis), but in contrast also show nanomolar affinity to AT-rich DNA in vitro (determined biochemically), which might be explained due to the extensive post-transcriptional modifications in vivo. (wikipedia.org)
  • HMGA1 preferentially binds to the minor groove of AT-rich regions in double-stranded DNA using its AT-hooks. (wikipedia.org)
  • HMGA1 proteins have high amounts of diverse posttranslational modifications and are located mainly in the nucleus, especially in heterochromatin, but also in mitochondria and the cytoplasm. (wikipedia.org)
  • mRNA
  • Human RAGE mRNA undergoes alternative splicing, much as with other proteins located within the MHC-III locus on chromosome 6. (biomedcentral.com)
  • It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. (uniprot.org)
  • As a result, these mRNA molecules are silenced, by one or more of the following processes: Cleavage of the mRNA strand into two pieces, Destabilization of the mRNA through shortening of its poly(A) tail, and Less efficient translation of the mRNA into proteins by ribosomes. (wikipedia.org)
  • isolated the lin-4 gene, they found that instead of producing an mRNA encoding a protein, it produced short non-coding RNAs, one of which was a ~22-nucleotide RNA that contained sequences partially complementary to multiple sequences in the 3' UTR of the lin-14 mRNA. (wikipedia.org)
  • This complementarity was proposed to inhibit the translation of the lin-14 mRNA into the LIN-14 protein. (wikipedia.org)
  • The protein appears to have important roles in neuronal development and mRNA transport. (wikipedia.org)
  • HMGA2
  • Subsequently, we generated an adenovirus containing the HMGA2 gene in the antisense orientation (Ad-A2as) to study the effect of HMGA2 protein suppression in ALT cells. (aacrjournals.org)
  • On the basis of these findings, the targeting of the HMGA2 protein expression may represent a promising approach for treating the well-differentiated liposarcomas resistant to conventional therapies. (aacrjournals.org)
  • Using transgenic mouse technology, they also showed (concurrently and independently of Alfredo Fusco), that fusion proteins between HMGA2 and other C terminal peptides (following chromosomal translocation) can drive the development of lipomas and generate obese mice. (wikipedia.org)
  • roles
  • Possible roles of this new AAA+ protein are discussed. (jove.com)
  • His research at UCLA has focused on roles of novel protein methyltransferases in aging and biological regulation highlighted by discoveries of the protein L-isoaspartyl repair methyltransferase, the isoprenylcysteine protein methyltransferase, and the protein phosphatase 2A methyltransferase. (ucla.edu)
  • The candidate sequences presented here represent important information for research into the various roles of HMGA1a, including cell differentiation, death, growth, proliferation, and the pathogenesis of cancer. (jove.com)
  • cellular
  • Pubmed ID: 16584891 We investigated a new archaeal member of the AAA+ protein family (ATPases associated with various cellular activities) which is found in all methanogenic archaea and the sulphate-reducer Archaeoglobus fulgidus. (jove.com)
  • sequence
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • However, in most other organisms, ORC lacks sequence‐specific DNA‐binding activity. (embopress.org)
  • Schizosaccharomyces pombe ORC lacks sequence‐specific binding, but the spORC4 subunit possesses a species‐specific AT‐hook domain that confers non‐specific binding to AT‐rich DNA ( Chuang and Kelly, 1999 ). (embopress.org)
  • metabolism
  • It is found either as a membrane-bound or soluble protein that is markedly upregulated by stress in epithelial cells, thereby regulating their metabolism and enhancing their central barrier functionality. (biomedcentral.com)
  • metastatic
  • Moreover, S100A13 was found to be involved in the invasiveness of lung cancer cell lines [ 10 ], and also was detected in tumor cells circulating in blood of patients with metastatic cancer [ 11 ], indicating that this protein may be considered as a predictor of cancer metastases. (biomedcentral.com)
  • Molecular
  • He obtained his PhD in Biochemistry and Molecular Biology at Harvard University working as an NSF Fellow with Professor Guido Guidotti on membrane protein-detergent interactions and the identification of the major rat liver mitochondrial polypeptides as enzymes of the urea cycle. (ucla.edu)
  • structural
  • Characterization of a New AAA+ Protein from Archaea Journal of Structural Biology. (jove.com)
  • The protein is most homologous to the AAA-domain of FtsH from bacteria, while the N-terminal domain shows predicted structural homology to members of the CDC48 family of AAA proteins. (jove.com)
  • complex
  • As suggested by homology to other members of the AAA+ family, the complex binds and hydrolyzes ATP. (jove.com)
  • domain
  • RAGE is expressed as both full-length, membrane-bound forms (fl-RAGE or mRAGE, not to be confused with mouse RAGE) and various soluble forms lacking the transmembrane domain. (biomedcentral.com)
  • p>This subsection of the 'Function' section specifies the position and type of each DNA-binding domain present within the protein. (uniprot.org)
  • HMGA1a, which can functionally substitute for LR1 and LR2 domain, can also recruit ORC in an RNA‐dependent manner. (embopress.org)
  • given
  • be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein. (uniprot.org)
  • different
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)